Csor.00g297280 (gene) Silver-seed gourd (wild; sororia) v1

BLAST of Csor.00g297280 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 1.0e-152
Identity = 268/522 (51.34%), Postives = 366/522 (70.11%), Query Frame = 0

Query: 28  VSANFGKPHENFLQCLSHYSQDGSSISNVLYTPFNSSYSNVLNFTLRNLRFSTPHTPKPL 87
           +  +   P ENFL+C S Y     + + ++YT  +  Y ++LN T++NLRF++  TPKPL
Sbjct: 23  IQISIANPQENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPL 82

Query: 88  LIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLSYVASLPFIIIDLINLNSISIDV 147
           +IITPL+VSHIQ  +LCSK  GLQIRTRSGGHD EG+SY++ +PF+I+DL N++S+ IDV
Sbjct: 83  VIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDV 142

Query: 148 ESKTAWVQSGTTLGELYYSIAKKSQTLTFPAGICPTVGVGGHFSGGGYGRLLRKYGLAAD 207
            S+TAWV++G TLGE+YY I + ++ L+FPAG CPTVG GGHFSGGGYG L+R YGLAAD
Sbjct: 143 HSQTAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAAD 202

Query: 208 NVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGGGSFGIVVAWKVKLVSVPASVTLCMVNK 267
           N+IDA LV+ +GK ++RK MGEDLFWAIRGGGG +FGI+ AWK++LV+VP+  T+  V K
Sbjct: 203 NIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKK 262

Query: 268 TSE-DGATKLIHQWQYVAHKLENDIFVGILLGGGNFT-RQG-DKINPVALFFSLYLGKAD 327
             E     KL+++WQ +A+  E ++ +       N T  QG +K    + F S++ G  D
Sbjct: 263 NMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVD 322

Query: 328 ELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGLQLGLQPGSKLEDLLSKTTPIIAST 387
            LV ++N +FPELG+ K DC + SW++T II  +G+        K E LL ++    A+ 
Sbjct: 323 SLVDLMNKSFPELGIKKTDCKQLSWIDT-IIFYSGVVNYNTTYFKKEILLDRSGGRKAAF 382

Query: 388 KIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFVPYGGRMGQISESETPFSHRGEYMY 447
            IK DYVK+PIP  A+  I E+L  +D+ +  ++F PYGG M +ISES  PF HR   MY
Sbjct: 383 SIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMY 442

Query: 448 KIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSKSPRAAYLNYRDLDIGSNNKYGRTS 507
           +I Y+  W +Q  +  ++HI+WIR +Y++ TP+VS++PR AYLNYRDLD+G  N     +
Sbjct: 443 EIWYIASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNN 502

Query: 508 YEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTL 547
           Y QA IWG KYFGK NF+RLV VKTKVDP +FFR+EQSIP L
Sbjct: 503 YTQARIWGEKYFGK-NFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Csor.00g297280 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 1.8e-152
Identity = 268/522 (51.34%), Postives = 365/522 (69.92%), Query Frame = 0

Query: 28  VSANFGKPHENFLQCLSHYSQDGSSISNVLYTPFNSSYSNVLNFTLRNLRFSTPHTPKPL 87
           +  +   P ENFL+CLS Y     + + ++YT  +  Y ++LN T++NLRF++  TPKPL
Sbjct: 23  IQISIANPQENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPL 82

Query: 88  LIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLSYVASLPFIIIDLINLNSISIDV 147
           +I TPL+VSHIQ  +LCSK  GLQIRTRSGGHD EG+SY++ +PF+I+DL N++S+ IDV
Sbjct: 83  VITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDV 142

Query: 148 ESKTAWVQSGTTLGELYYSIAKKSQTLTFPAGICPTVGVGGHFSGGGYGRLLRKYGLAAD 207
            S+TAWV+SG TLGE+YY I + ++ L+FPAG CPTVG GGHFSGGGYG L+R YGLAAD
Sbjct: 143 HSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAAD 202

Query: 208 NVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGGGSFGIVVAWKVKLVSVPASVTLCMVNK 267
           N+IDA LV+ +GK ++RK MGEDLFWAIRGGGG +FGI+ AWK++LV+VP+  T+  V K
Sbjct: 203 NIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKK 262

Query: 268 TSE-DGATKLIHQWQYVAHKLENDIFVGILLGGGNFT-RQG-DKINPVALFFSLYLGKAD 327
             E     KL+++WQ +A+  E ++ +       N T  QG +K    + F S++ G  D
Sbjct: 263 NMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVD 322

Query: 328 ELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGLQLGLQPGSKLEDLLSKTTPIIAST 387
            LV ++N +FPELG+ K DC + SW++T II  +G+        K E LL ++    A+ 
Sbjct: 323 SLVDLMNKSFPELGIKKTDCKQLSWIDT-IIFYSGVVNYNTTNFKKEILLDRSGGRKAAF 382

Query: 388 KIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFVPYGGRMGQISESETPFSHRGEYMY 447
            IK DYVK+PIP  A+  I E+L  +D+ +  ++F PYGG M +ISES  PF HR    Y
Sbjct: 383 SIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITY 442

Query: 448 KIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSKSPRAAYLNYRDLDIGSNNKYGRTS 507
           +I Y+  W +Q  +  ++HI+WIR +Y++ TP+VS++PR AYLNYRDLD+G  N     +
Sbjct: 443 EIWYIASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNN 502

Query: 508 YEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTL 547
           Y QA IWG KYFGK NF+RLV VKTKVDP +FFR+EQSIP L
Sbjct: 503 YTQARIWGEKYFGK-NFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Csor.00g297280 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 7.5e-151
Identity = 268/544 (49.26%), Postives = 366/544 (67.28%), Query Frame = 0

Query: 6   MNSSSLLPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTPFNSSY 65
           MN S+     +  ++    S  +  +   P ENFL+C S +  +  +   ++YT  +  Y
Sbjct: 1   MNCSAFSFWFVCKIIFFFLSFHIQISIANPRENFLKCFSKHIPNNVANPKLVYTQHDQLY 60

Query: 66  SNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLS 125
            ++LN T++NLRF +  TPKPL+I+TP + SHIQA +LCSK  GLQIRTRSGGHD EG+S
Sbjct: 61  MSILNSTIQNLRFISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMS 120

Query: 126 YVASLPFIIIDLINLNSISIDVESKTAWVQSGTTLGELYYSIAKKSQTLTFPAGICPTVG 185
           Y++ +PF+++DL N++SI IDV S+TAWV++G TLGE+YY I +K++ L+FP G CPTVG
Sbjct: 121 YISQVPFVVVDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVG 180

Query: 186 VGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGGGSFGI 245
           VGGHFSGGGYG L+R YGLAADN+IDA LV+ +GK ++RK MGEDLFWAIRGGGG +FGI
Sbjct: 181 VGGHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGI 240

Query: 246 VVAWKVKLVSVPASVTLCMVNKTSE-DGATKLIHQWQYVAHKLENDIFVGILLGGGNFT- 305
           + AWK+KLV+VP+  T+  V K  E  G  KL ++WQ +A+K + D+ +       N T 
Sbjct: 241 IAAWKIKLVAVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITD 300

Query: 306 -RQGDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGLQL 365
               +K      F S++ G  D LV ++N +FPELG+ K DC E SW++T I   +G+  
Sbjct: 301 NHGKNKTTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFY-SGVVN 360

Query: 366 GLQPGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFVPY 425
                 K E LL ++     +  IK DYVK+PIP  A+  I E+L  +D+    Y+  PY
Sbjct: 361 FNTANFKKEILLDRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPY 420

Query: 426 GGRMGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSKSP 485
           GG M +ISES  PF HR   MY++ Y   W +Q  +  ++HI+W+R +Y++ TP+VS++P
Sbjct: 421 GGIMEEISESAIPFPHRAGIMYELWYTASWEKQ--EDNEKHINWVRSVYNFTTPYVSQNP 480

Query: 486 RAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQS 545
           R AYLNYRDLD+G  N     +Y QA IWG KYFGK NF+RLV VKTKVDP +FFR+EQS
Sbjct: 481 RLAYLNYRDLDLGKTNHASPNNYTQARIWGEKYFGK-NFNRLVKVKTKVDPNNFFRNEQS 540

Query: 546 IPTL 547
           IP L
Sbjct: 541 IPPL 540

BLAST of Csor.00g297280 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 1.3e-150
Identity = 266/522 (50.96%), Postives = 357/522 (68.39%), Query Frame = 0

Query: 28  VSANFGKPHENFLQCLSHYSQDGSSISNVLYTPFNSSYSNVLNFTLRNLRFSTPHTPKPL 87
           +  +   P ENFL+C S Y  + ++   ++YT  N  Y +VLN T+ NLRF++  TPKPL
Sbjct: 23  IQTSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPL 82

Query: 88  LIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLSYVASLPFIIIDLINLNSISIDV 147
           +I+TP HVSHIQ  +LCSK  GLQIRTRSGGHD EG+SY++ +PF+I+DL N+ SI IDV
Sbjct: 83  VIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDV 142

Query: 148 ESKTAWVQSGTTLGELYYSIAKKSQTLTFPAGICPTVGVGGHFSGGGYGRLLRKYGLAAD 207
            S+TAWV++G TLGE+YY + +K++ L+  AG CPTV  GGHF GGGYG L+R YGLAAD
Sbjct: 143 HSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAAD 202

Query: 208 NVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGGGSFGIVVAWKVKLVSVPASVTLCMVNK 267
           N+IDA LV+ +GK ++RK MGEDLFWA+RGGG  SFGI+VAWK++LV+VP S T+  V K
Sbjct: 203 NIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPKS-TMFSVKK 262

Query: 268 TSE-DGATKLIHQWQYVAHKLENDIFVGILLGGGNFT-RQGDKINPVALFF-SLYLGKAD 327
             E     KL+++WQ +A+K + D+ +       N T  QG     +  +F S++LG  D
Sbjct: 263 IMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGKNKTAIHTYFSSVFLGGVD 322

Query: 328 ELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGLQLGLQPGSKLEDLLSKTTPIIAST 387
            LV ++N +FPELG+ K DC + SW++T I     +       +K E LL ++     + 
Sbjct: 323 SLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNK-EILLDRSAGQNGAF 382

Query: 388 KIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFVPYGGRMGQISESETPFSHRGEYMY 447
           KIK DYVK+PIP +    I E+L  +DI    Y   PYGG M +ISES  PF HR   +Y
Sbjct: 383 KIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIPFPHRAGILY 442

Query: 448 KIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSKSPRAAYLNYRDLDIGSNNKYGRTS 507
           ++ Y+  W +Q  +  ++H++WIR +Y++MTP+VSK+PR AYLNYRDLDIG N+     +
Sbjct: 443 ELWYICSWEKQ--EDNEKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGINDPKNPNN 502

Query: 508 YEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTL 547
           Y QA IWG KYFGK NFDRLV VKT VDP +FFR+EQSIP L
Sbjct: 503 YTQARIWGEKYFGK-NFDRLVKVKTLVDPNNFFRNEQSIPPL 539

BLAST of Csor.00g297280 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 4.8e-150
Identity = 265/544 (48.71%), Postives = 364/544 (66.91%), Query Frame = 0

Query: 6   MNSSSLLPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTPFNSSY 65
           MN S+     +  ++    S  +  +   P ENFL+C S Y  +  +    +YT  +  Y
Sbjct: 1   MNCSAFSFWFVCKIIFFFLSFNIQISIANPQENFLKCFSEYIPNNPANPKFIYTQHDQLY 60

Query: 66  SNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLS 125
            +VLN T++NLRF++  TPKPL+I+TP +VSHIQA++LCSK  GLQIRTRSGGHD EGLS
Sbjct: 61  MSVLNSTIQNLRFTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLS 120

Query: 126 YVASLPFIIIDLINLNSISIDVESKTAWVQSGTTLGELYYSIAKKSQTLTFPAGICPTVG 185
           Y++ +PF I+DL N++++ +D+ S+TAWV++G TLGE+YY I + ++  +FP G CPTVG
Sbjct: 121 YISQVPFAIVDLRNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVG 180

Query: 186 VGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGGGSFGI 245
           VGGHFSGGGYG L+R YGLAADN+IDA LV+ +GK ++RK MGEDLFWAIRGGGG +FGI
Sbjct: 181 VGGHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGI 240

Query: 246 VVAWKVKLVSVPASVTLCMVNKTSE-DGATKLIHQWQYVAHKLENDIFVGILLGGGNFT- 305
           + AWK+KLV VP+  T+  V K  E  G  KL ++WQ +A+K + D+ +       N T 
Sbjct: 241 IAAWKIKLVVVPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITD 300

Query: 306 -RQGDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGLQL 365
               +K      F S++LG  D LV ++N +FPELG+ K DC E SW++T I   +G+  
Sbjct: 301 NHGKNKTTVHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFY-SGVVN 360

Query: 366 GLQPGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFVPY 425
                 K E LL ++     +  IK DYVK+ IP  A+  I E+L  +++ +  Y+  PY
Sbjct: 361 YNTANFKKEILLDRSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPY 420

Query: 426 GGRMGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSKSP 485
           GG M +ISES  PF HR   MY++ Y   W +Q  +  ++HI+W+R +Y++ TP+VS++P
Sbjct: 421 GGIMDEISESAIPFPHRAGIMYELWYTATWEKQ--EDNEKHINWVRSVYNFTTPYVSQNP 480

Query: 486 RAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQS 545
           R AYLNYRDLD+G  N     +Y QA IWG KYFGK NF+RLV VKTK DP +FFR+EQS
Sbjct: 481 RLAYLNYRDLDLGKTNPESPNNYTQARIWGEKYFGK-NFNRLVKVKTKADPNNFFRNEQS 540

Query: 546 IPTL 547
           IP L
Sbjct: 541 IPPL 540

BLAST of Csor.00g297280 vs. NCBI nr
Match: KAG6573424.1 (Cannabidiolic acid synthase-like 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1112 bits (2875), Expect = 0.0
Identity = 549/549 (100.00%), Postives = 549/549 (100.00%), Query Frame = 0

Query: 1   MSNSAMNSSSLLPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTP 60
           MSNSAMNSSSLLPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTP
Sbjct: 1   MSNSAMNSSSLLPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTP 60

Query: 61  FNSSYSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIIDLINLNSISIDVESKTAWVQSGTTLGELYYSIAKKSQTLTFPAGI 180
           FEGLSYVASLPFIIIDLINLNSISIDVESKTAWVQSGTTLGELYYSIAKKSQTLTFPAGI
Sbjct: 121 FEGLSYVASLPFIIIDLINLNSISIDVESKTAWVQSGTTLGELYYSIAKKSQTLTFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG 240
           CPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFVGILLGGG 300
           GSFGIVVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFVGILLGGG
Sbjct: 241 GSFGIVVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFVGILLGGG 300

Query: 301 NFTRQGDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGL 360
           NFTRQGDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGL
Sbjct: 301 NFTRQGDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGL 360

Query: 361 QLGLQPGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFV 420
           QLGLQPGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFV
Sbjct: 361 QLGLQPGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFV 420

Query: 421 PYGGRMGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSK 480
           PYGGRMGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSK
Sbjct: 421 PYGGRMGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSK 480

Query: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 540
           SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE
Sbjct: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 540

Query: 541 QSIPTLSSK 549
           QSIPTLSSK
Sbjct: 541 QSIPTLSSK 549

BLAST of Csor.00g297280 vs. NCBI nr
Match: XP_022954563.1 (berberine bridge enzyme-like 18 [Cucurbita moschata])

HSP 1 Score: 1103 bits (2852), Expect = 0.0
Identity = 543/549 (98.91%), Postives = 546/549 (99.45%), Query Frame = 0

Query: 1   MSNSAMNSSSLLPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTP 60
           MSNSAMNSS LLPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTP 60

Query: 61  FNSSYSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIIDLINLNSISIDVESKTAWVQSGTTLGELYYSIAKKSQTLTFPAGI 180
           FEGLSYVASLPFII+DLINLNSISIDV+SKTAWVQSG TLGELYYSIAKKSQTLTFPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVKSKTAWVQSGATLGELYYSIAKKSQTLTFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG 240
           CPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFVGILLGGG 300
           GSFGIVVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIF+GILLGGG
Sbjct: 241 GSFGIVVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFLGILLGGG 300

Query: 301 NFTRQGDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGL 360
           NFTRQGDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGL
Sbjct: 301 NFTRQGDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGL 360

Query: 361 QLGLQPGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFV 420
           QLGLQPGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFV
Sbjct: 361 QLGLQPGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFV 420

Query: 421 PYGGRMGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSK 480
           PYGGRMGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSK
Sbjct: 421 PYGGRMGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSK 480

Query: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 540
           SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFG ENFDRLVHVKTKVDPYDFFRHE
Sbjct: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGNENFDRLVHVKTKVDPYDFFRHE 540

Query: 541 QSIPTLSSK 549
           QSIPTLSSK
Sbjct: 541 QSIPTLSSK 549

BLAST of Csor.00g297280 vs. NCBI nr
Match: XP_023542821.1 (berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1079 bits (2790), Expect = 0.0
Identity = 528/544 (97.06%), Postives = 536/544 (98.53%), Query Frame = 0

Query: 6   MNSSSLLPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTPFNSSY 65
           MNSS LLPLSLTLLVLCSCSSWVSANFGKPH+NFLQCLSHYSQDGSSISNVLYTPFNSSY
Sbjct: 1   MNSSPLLPLSLTLLVLCSCSSWVSANFGKPHQNFLQCLSHYSQDGSSISNVLYTPFNSSY 60

Query: 66  SNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLS 125
           SNVLNFTLRNLRFSTP TPKPLLIITPLHVSHIQAAVLCSKSH LQIRTRSGGHDFEGLS
Sbjct: 61  SNVLNFTLRNLRFSTPQTPKPLLIITPLHVSHIQAAVLCSKSHSLQIRTRSGGHDFEGLS 120

Query: 126 YVASLPFIIIDLINLNSISIDVESKTAWVQSGTTLGELYYSIAKKSQTLTFPAGICPTVG 185
           YVASLPFII+DLINLNSISIDVESKTAWVQSG TLGELYYSIAKKSQTLTFPAGICPTVG
Sbjct: 121 YVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLTFPAGICPTVG 180

Query: 186 VGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGGGSFGI 245
           VGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGGGSFGI
Sbjct: 181 VGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGGGSFGI 240

Query: 246 VVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFVGILLGGGNFTRQ 305
           VVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIF+GILLGGGNFTRQ
Sbjct: 241 VVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFLGILLGGGNFTRQ 300

Query: 306 GDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGLQLGLQ 365
           GDKINP+ALFFSLYLGKADELVAILNTTFPELGLSKK+CMETSW+ETAIIMGTGLQ GLQ
Sbjct: 301 GDKINPIALFFSLYLGKADELVAILNTTFPELGLSKKECMETSWIETAIIMGTGLQPGLQ 360

Query: 366 PGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFVPYGGR 425
           PGSKLEDLLSKTTP+IASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQY+FVPYGGR
Sbjct: 361 PGSKLEDLLSKTTPVIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYLFVPYGGR 420

Query: 426 MGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSKSPRAA 485
           MGQISESETPFSHRGEYMYKIGY VGWVE GIDAEKRHIDWIR LYSYMTPFVSKSPRAA
Sbjct: 421 MGQISESETPFSHRGEYMYKIGYTVGWVEGGIDAEKRHIDWIRALYSYMTPFVSKSPRAA 480

Query: 486 YLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPT 545
           YLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPT
Sbjct: 481 YLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPT 540

Query: 546 LSSK 549
           LSSK
Sbjct: 541 LSSK 544

BLAST of Csor.00g297280 vs. NCBI nr
Match: XP_022994834.1 (berberine bridge enzyme-like 18 [Cucurbita maxima])

HSP 1 Score: 1058 bits (2736), Expect = 0.0
Identity = 520/549 (94.72%), Postives = 533/549 (97.09%), Query Frame = 0

Query: 1   MSNSAMNSSSLLPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTP 60
           MSNSAMNSS LLPLSLTLLVLCSCSSWVS+NF KPH+NFLQCLSHYSQDGSSIS+VLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60

Query: 61  FNSSYSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYS+VLN TLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIIDLINLNSISIDVESKTAWVQSGTTLGELYYSIAKKSQTLTFPAGI 180
           FEGLSYVASLPFII+DLI LNSISIDVESKTAWVQSG TLGELYYSIAKKSQTL FPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLIKLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG 240
           CPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNR+LMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRELMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFVGILLGGG 300
           GSFGIVVAWKV LVSVPASVTLCM +KTSEDGATKLIHQWQYVAHKLENDIF+GILLGGG
Sbjct: 241 GSFGIVVAWKVNLVSVPASVTLCMASKTSEDGATKLIHQWQYVAHKLENDIFLGILLGGG 300

Query: 301 NFTRQGDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGL 360
           NF+RQGDKINPVALFFSLYLGKADELVAIL+T FPELGLSKKDCMETSWVETAIIMGTGL
Sbjct: 301 NFSRQGDKINPVALFFSLYLGKADELVAILDTKFPELGLSKKDCMETSWVETAIIMGTGL 360

Query: 361 QLGLQPGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFV 420
           Q GLQPGSKLEDL SKTTPI ASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQ++FV
Sbjct: 361 QPGLQPGSKLEDLQSKTTPINASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQHLFV 420

Query: 421 PYGGRMGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSK 480
           PYGGRMGQI E ETPFSHRGEYMYKIGY VGWVE GIDAEKRHIDWIRE+YSYMTPFVSK
Sbjct: 421 PYGGRMGQIQEFETPFSHRGEYMYKIGYTVGWVEGGIDAEKRHIDWIREIYSYMTPFVSK 480

Query: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 540
           SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE
Sbjct: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 540

Query: 541 QSIPTLSSK 549
           QSIPTLSSK
Sbjct: 541 QSIPTLSSK 549

BLAST of Csor.00g297280 vs. NCBI nr
Match: KAG6573426.1 (Cannabidiolic acid synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1026 bits (2652), Expect = 0.0
Identity = 502/549 (91.44%), Postives = 524/549 (95.45%), Query Frame = 0

Query: 1   MSNSAMNSSSLLPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTP 60
           MSNSAMNSSSLLPLSLTLLVLCSCSSWVS+NF KPH+NFLQCLSHYSQDGSSIS+VLYTP
Sbjct: 1   MSNSAMNSSSLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60

Query: 61  FNSSYSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNS+YS+VLN TLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSTYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIIDLINLNSISIDVESKTAWVQSGTTLGELYYSIAKKSQTLTFPAGI 180
           FEGLSYVASLPFII+DLINLNSISIDVESKTAWVQSG TLGELYYSIAKKSQTL FPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG 240
           CPTVGVGGHFSGGGYG LLRKYGLAADNVIDAQLVDANGKFV+RKLMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVDRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFVGILLGGG 300
           GSFGIVVAWKVKLV VPASVTLCM NKTSEDGATKLIHQWQYVAHKLEN+ F+GILL GG
Sbjct: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300

Query: 301 NFTRQGDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGL 360
           NFTRQG KINP+ALFFSLYLGKADEL+ ILNTTFPELGLSKKDC+ETSWVETA+IMGTGL
Sbjct: 301 NFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCLETSWVETALIMGTGL 360

Query: 361 QLGLQPGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFV 420
           Q G    SKLEDLLSK T +  STKIKSDY+KEPIPMAAIEGIWERLR +DIE+PQ+IFV
Sbjct: 361 QFG----SKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFV 420

Query: 421 PYGGRMGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSK 480
           PYGG+MGQI E ETPFSHRGEY+YKIGY+V W EQGI+AEKRHIDWIRELYSYMTPFVSK
Sbjct: 421 PYGGKMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSK 480

Query: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 540
           SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE
Sbjct: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 540

Query: 541 QSIPTLSSK 549
           QSIPTLSSK
Sbjct: 541 QSIPTLSSK 545

BLAST of Csor.00g297280 vs. ExPASy TrEMBL
Match: A0A6J1GRG1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456798 PE=3 SV=1)

HSP 1 Score: 1103 bits (2852), Expect = 0.0
Identity = 543/549 (98.91%), Postives = 546/549 (99.45%), Query Frame = 0

Query: 1   MSNSAMNSSSLLPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTP 60
           MSNSAMNSS LLPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTP 60

Query: 61  FNSSYSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIIDLINLNSISIDVESKTAWVQSGTTLGELYYSIAKKSQTLTFPAGI 180
           FEGLSYVASLPFII+DLINLNSISIDV+SKTAWVQSG TLGELYYSIAKKSQTLTFPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVKSKTAWVQSGATLGELYYSIAKKSQTLTFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG 240
           CPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFVGILLGGG 300
           GSFGIVVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIF+GILLGGG
Sbjct: 241 GSFGIVVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFLGILLGGG 300

Query: 301 NFTRQGDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGL 360
           NFTRQGDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGL
Sbjct: 301 NFTRQGDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGL 360

Query: 361 QLGLQPGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFV 420
           QLGLQPGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFV
Sbjct: 361 QLGLQPGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFV 420

Query: 421 PYGGRMGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSK 480
           PYGGRMGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSK
Sbjct: 421 PYGGRMGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSK 480

Query: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 540
           SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFG ENFDRLVHVKTKVDPYDFFRHE
Sbjct: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGNENFDRLVHVKTKVDPYDFFRHE 540

Query: 541 QSIPTLSSK 549
           QSIPTLSSK
Sbjct: 541 QSIPTLSSK 549

BLAST of Csor.00g297280 vs. ExPASy TrEMBL
Match: A0A6J1K3Z1 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111490441 PE=3 SV=1)

HSP 1 Score: 1058 bits (2736), Expect = 0.0
Identity = 520/549 (94.72%), Postives = 533/549 (97.09%), Query Frame = 0

Query: 1   MSNSAMNSSSLLPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTP 60
           MSNSAMNSS LLPLSLTLLVLCSCSSWVS+NF KPH+NFLQCLSHYSQDGSSIS+VLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60

Query: 61  FNSSYSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYS+VLN TLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIIDLINLNSISIDVESKTAWVQSGTTLGELYYSIAKKSQTLTFPAGI 180
           FEGLSYVASLPFII+DLI LNSISIDVESKTAWVQSG TLGELYYSIAKKSQTL FPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLIKLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG 240
           CPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNR+LMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRELMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFVGILLGGG 300
           GSFGIVVAWKV LVSVPASVTLCM +KTSEDGATKLIHQWQYVAHKLENDIF+GILLGGG
Sbjct: 241 GSFGIVVAWKVNLVSVPASVTLCMASKTSEDGATKLIHQWQYVAHKLENDIFLGILLGGG 300

Query: 301 NFTRQGDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGL 360
           NF+RQGDKINPVALFFSLYLGKADELVAIL+T FPELGLSKKDCMETSWVETAIIMGTGL
Sbjct: 301 NFSRQGDKINPVALFFSLYLGKADELVAILDTKFPELGLSKKDCMETSWVETAIIMGTGL 360

Query: 361 QLGLQPGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFV 420
           Q GLQPGSKLEDL SKTTPI ASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQ++FV
Sbjct: 361 QPGLQPGSKLEDLQSKTTPINASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQHLFV 420

Query: 421 PYGGRMGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSK 480
           PYGGRMGQI E ETPFSHRGEYMYKIGY VGWVE GIDAEKRHIDWIRE+YSYMTPFVSK
Sbjct: 421 PYGGRMGQIQEFETPFSHRGEYMYKIGYTVGWVEGGIDAEKRHIDWIREIYSYMTPFVSK 480

Query: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 540
           SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE
Sbjct: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 540

Query: 541 QSIPTLSSK 549
           QSIPTLSSK
Sbjct: 541 QSIPTLSSK 549

BLAST of Csor.00g297280 vs. ExPASy TrEMBL
Match: A0A6J1K2F5 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111490442 PE=3 SV=1)

HSP 1 Score: 1024 bits (2647), Expect = 0.0
Identity = 502/549 (91.44%), Postives = 522/549 (95.08%), Query Frame = 0

Query: 1   MSNSAMNSSSLLPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTP 60
           MSNSAMNSS LLPLSLTLLVLCSCSSWVS+NF KPH+NFLQCLSHYSQDGSSIS+VLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60

Query: 61  FNSSYSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYS+VLN TLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIIDLINLNSISIDVESKTAWVQSGTTLGELYYSIAKKSQTLTFPAGI 180
           FEGLSYVASLPFII+DLINLNSISIDVESKTAWVQSG TLGELYY IAKKS+TL FPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAKKSRTLAFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG 240
           CPTVGVGGHFSGGGYG LLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFVGILLGGG 300
           GSFGIVVAWKVKLV VPASVTLCM NKTSEDGATKLIHQWQYVAHKLEN+ F+GILL GG
Sbjct: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300

Query: 301 NFTRQGDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGL 360
           NFTRQG KINP+ALFFSLYLGKADEL+ ILNTTFPELGLSKKDCMETSWVETA+IMGTGL
Sbjct: 301 NFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL 360

Query: 361 QLGLQPGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFV 420
           QLG    SKLEDLLSK   +  STKIKSDY+KEPIPMAAIEGIWERLR +DIE+PQ+IFV
Sbjct: 361 QLG----SKLEDLLSKAPLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFV 420

Query: 421 PYGGRMGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSK 480
           PYGG+MGQI E ETPFSHRGEY+YKIGY+V W EQGI+AEKRHIDWIRELYSYMTPFVSK
Sbjct: 421 PYGGKMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSK 480

Query: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 540
           SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE
Sbjct: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 540

Query: 541 QSIPTLSSK 549
           QSIPTLSSK
Sbjct: 541 QSIPTLSSK 545

BLAST of Csor.00g297280 vs. ExPASy TrEMBL
Match: A0A6J1GRE1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456783 PE=3 SV=1)

HSP 1 Score: 1021 bits (2641), Expect = 0.0
Identity = 501/549 (91.26%), Postives = 523/549 (95.26%), Query Frame = 0

Query: 1   MSNSAMNSSSLLPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTP 60
           MSNSAMNSS LLPLSLTLLVLCSCSSWVS+NF KPH+NFLQCLSHYSQDGSSIS+VLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60

Query: 61  FNSSYSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYS+VLN TLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIIDLINLNSISIDVESKTAWVQSGTTLGELYYSIAKKSQTLTFPAGI 180
           FEGLSYVASLPFII+DLINLNSISIDVE+KTAWVQSG TLGELYYSIAKKSQTL FPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG 240
           CPTVGVGGHFSGGGYG LLRKYGLAADNVIDAQLVDANGKFVNR+LMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFVGILLGGG 300
           GSFGIVVAWKVKLV VPASVTLCM NKTSEDGATKLIHQWQYVAHKLEN+ F+GILL GG
Sbjct: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300

Query: 301 NFTRQGDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGL 360
           NFTRQG KINP+ALFFSLYLGKA+EL+ ILNTTFPELGLSKKDCMETSWVETA+IMGTGL
Sbjct: 301 NFTRQGGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL 360

Query: 361 QLGLQPGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFV 420
           QLG    SKLEDLLSK T +  STKIKSDY+KEPIPMAAIEGIWERLR +DIE+PQ+IFV
Sbjct: 361 QLG----SKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFV 420

Query: 421 PYGGRMGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSK 480
           PYGG+MGQI E ETPFSHRGE +YKIGY+V W EQGI+AEKRHIDWIRELYSYMTPFVSK
Sbjct: 421 PYGGKMGQIPEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSK 480

Query: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 540
           SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE
Sbjct: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 540

Query: 541 QSIPTLSSK 549
           QSIPTLSSK
Sbjct: 541 QSIPTLSSK 545

BLAST of Csor.00g297280 vs. ExPASy TrEMBL
Match: A0A6J1GRE5 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456787 PE=3 SV=1)

HSP 1 Score: 988 bits (2553), Expect = 0.0
Identity = 484/549 (88.16%), Postives = 510/549 (92.90%), Query Frame = 0

Query: 1   MSNSAMNSSSLLPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTP 60
           MSNSAMNSS LLPLSLTLLVLCSCSSWVS+NF KPHENFLQCLSHYSQD SSISNVLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTP 60

Query: 61  FNSSYSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSSYS+VLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIIDLINLNSISIDVESKTAWVQSGTTLGELYYSIAKKSQTLTFPAGI 180
            EGLSYVASLPFII+DLINLNSISIDVESKTAWVQSG TLGELYY IA+KS++L FPAGI
Sbjct: 121 IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG 240
           CPTVGVGGH SGGGYG LLRKYGLAADNVIDAQLVDANGKF +RKLMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFVGILLGGG 300
           GSFGIVVAWKV LVSVPA+VTLC+VNKT EDGATKLIHQWQYVAHKLEN++F+GILL GG
Sbjct: 241 GSFGIVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLGILLEGG 300

Query: 301 NFTRQGDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGL 360
           NFTRQG KINP+ALFFS YLGKADEL+ ILNTTFPEL LSKKDC+ETSWVETA+IMGTGL
Sbjct: 301 NFTRQGSKINPLALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGTGL 360

Query: 361 QLGLQPGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFV 420
           Q    PGSKLEDLLSKT  +  STK+KSDYVKEPIPMAAIEGI +RLRS DIE PQ IF+
Sbjct: 361 Q----PGSKLEDLLSKTPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFI 420

Query: 421 PYGGRMGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSK 480
           PYGG+M +ISE ETPFSHRGEY YKIGY   WVE+GIDAE +H++WIRELYSYM PFVSK
Sbjct: 421 PYGGKMSEISELETPFSHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSK 480

Query: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 540
           SPRAAYLNYRDLDIGSNNKYGRTSYEQA IWGYKYFGKENFDRLVHVKTKVDPYDFFRHE
Sbjct: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 540

Query: 541 QSIPTLSSK 549
           QSIPT SSK
Sbjct: 541 QSIPTFSSK 545

BLAST of Csor.00g297280 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 529.3 bits (1362), Expect = 3.8e-150
Identity = 269/536 (50.19%), Postives = 371/536 (69.22%), Query Frame = 0

Query: 9   SSLLPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTPFNSSYSNV 68
           SS+L    T  +L S    VSAN      +FLQCLS    D + +S V++TP ++S+S+V
Sbjct: 8   SSVLIFFTTSTLLLSIPHPVSANRSN-QSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSV 67

Query: 69  LNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLSYVA 128
           L  +++N RFS P  PKP+LI+TP+  S +Q+AV C++  G+ IRTRSGGHD+EGLSYV 
Sbjct: 68  LASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVT 127

Query: 129 SLPFIIIDLINLNSISIDVESKTAWVQSGTTLGELYYSIAKKSQTLTFPAGICPTVGVGG 188
             PF+I+DL NL SI++DV++++ WVQ+G T+GELYY I KK++TL FPAG+CPTVGVGG
Sbjct: 128 HKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGG 187

Query: 189 HFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGGGSFGIVVA 248
           HFSGGGYG LLRK+GLAAD+VIDA++VDA G+ + R+ MGED FWAIRGGGG SF +V++
Sbjct: 188 HFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLS 247

Query: 249 WKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFVGILLGGGNFTRQGDK 308
           WK+ L++VP++VT+  V K SE  A K+IH+WQ+VA K+ +D+F+ ++L       Q  K
Sbjct: 248 WKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML-------QRYK 307

Query: 309 INPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGLQLGLQPGS 368
               A F  LYLG    L+ ++N  FPELGL + DC E SW+E+ I      +LG +P  
Sbjct: 308 NMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA---ELGEEP-- 367

Query: 369 KLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFVPYGGRMGQ 428
              ++L+K T    + K KSD+V+EP+P  AI  +W RL+  + E  Q IF P+GG+M +
Sbjct: 368 --INVLTKRTRASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSE 427

Query: 429 ISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSKSPRAAYLN 488
           I++ ETPF HR   +Y+I Y+  W  +G D +++++ W+  +Y  M+ FV+KSPR AY+N
Sbjct: 428 IADYETPFPHRKGNIYEIQYLNYW--RG-DVKEKYMRWVERVYDDMSEFVAKSPRGAYIN 487

Query: 489 YRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 545
            RDLD+G      R+ YE+   WG KYF K NF+RLV VKT VDP DFF  EQSIP
Sbjct: 488 LRDLDLGMYVGVKRSKYEEGKSWGVKYF-KNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of Csor.00g297280 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 513.8 bits (1322), Expect = 1.7e-145
Identity = 257/535 (48.04%), Postives = 361/535 (67.48%), Query Frame = 0

Query: 15  SLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTPFNSSYSNVLNFTLR 74
           +L L++      W S++     E F QCL+  S     IS  ++   N SYS+VL   +R
Sbjct: 4   ALILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIR 63

Query: 75  NLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLSYV--ASLPF 134
           NLRF+T  TPKP LII   H SH+QAA+ C K H LQ++ RSGGHD++GLSYV  +  PF
Sbjct: 64  NLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPF 123

Query: 135 IIIDLINLNSISIDVESKTAWVQSGTTLGELYYSIAKKSQTLTFPAGICPTVGVGGHFSG 194
            ++D+ NL S+ +DV SKTAWVQ+G  LGE+YY I +KS+TL +PAGICPTVGVGGH SG
Sbjct: 124 FVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISG 183

Query: 195 GGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGGGSFGIVVAWKVK 254
           GGYG ++RKYGL  DN IDA++VD NGK ++RKLMGEDL+WAI GGGGGS+G+V+A+K+ 
Sbjct: 184 GGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKIN 243

Query: 255 LVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFVGILLGGGNFTRQGDKINPV 314
           LV VP +VT+  +++T E  AT +IH+WQ VA KL +++F+  ++   N T    K    
Sbjct: 244 LVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQK-TVR 303

Query: 315 ALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGLQLGLQPGSKLED 374
             F +++LG    L++ILN  FPELGL + DC ETSW++ +++  T +Q+G       E 
Sbjct: 304 TTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQ-SVLFWTNIQVG-----SSET 363

Query: 375 LLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFVPYGGRMGQISES 434
           LL +    +   K KSDYV+EPI    +E IW+++   ++E+P   F PYGG MG+IS +
Sbjct: 364 LLLQRNQPVNYLKRKSDYVREPISRTGLESIWKKM--IELEIPTMAFNPYGGEMGRISST 423

Query: 435 ETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSKSPRAAYLNYRDL 494
            TPF +R   ++KI Y   W ++ +    R+++  R+LY +MTPFVSK+PR ++ NYRD+
Sbjct: 424 VTPFPYRAGNLWKIQYGANWRDETL--TDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDV 483

Query: 495 DIGSNNKYGR-TSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTL 547
           D+G N+  G+ +SY +   +G KYF   NF+RLV +KT+VD  +FFR+EQSIP L
Sbjct: 484 DLGINSHNGKISSYVEGKRYGKKYFA-GNFERLVKIKTRVDSGNFFRNEQSIPVL 526

BLAST of Csor.00g297280 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 513.8 bits (1322), Expect = 1.7e-145
Identity = 259/535 (48.41%), Postives = 363/535 (67.85%), Query Frame = 0

Query: 18  LLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTPFNSSYSNVLNFTLRNLR 77
           LL   + S  VSA+ G  HE+FL+CLS+   D +    V++T  +SS+ ++L+ +++N R
Sbjct: 11  LLFSLNISPLVSAH-GSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSIQNPR 70

Query: 78  FSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLSYVA-SLPFIIID 137
           FS   TPKP+ IITP+  S +Q  + C++ HG+ +RTRS GH +EGLSY+A + PF +ID
Sbjct: 71  FSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVID 130

Query: 138 LINLNSISIDVESKTAWVQSGTTLGELYYSIAKKSQTLTFPAGICPTVGVGGHFSGGGYG 197
           L NL SIS+DV+++T WVQ+G T GELYY I K +++L FPAGI PTVGVGG FSGGGYG
Sbjct: 131 LRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYG 190

Query: 198 RLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGGGSFGIVVAWKVKLVSV 257
            LLRKYGLAADN+IDA +VDA+G+ ++R+ MGED FWAIRGGGG SFG++++WKVKLV V
Sbjct: 191 TLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDV 250

Query: 258 PASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFVGILLGGGNFTRQGDKINPVALFF 317
           P+++T+  V KTS+  A ++I +WQY A K+ +D+F+   L       + +K    ALF 
Sbjct: 251 PSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTL------ERSNKNAVHALFT 310

Query: 318 SLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGLQLGLQPGSKLEDLLSK 377
            LY+G  + L+A++   FPELGL K+ C E SW+E+       L     P  +   +L+ 
Sbjct: 311 GLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESV------LWFADFPKGESLGVLTN 370

Query: 378 TTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFVPYGGRMGQISESETPF 437
                 S K K D+V+EPIP AAI+ IW RL + +  L + I  P+GG+M +++E ETPF
Sbjct: 371 RERTSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPF 430

Query: 438 SHRGEYMYKIGYVVGWVEQGIDAEK----RHIDWIRELYSYMTPFVSKSPRAAYLNYRDL 497
            HRG  +Y+I YV  W E+  D  K    +++ W+  +Y +MTP+VSKSPR AY+N++D+
Sbjct: 431 PHRGGNLYEIQYVAYWREEE-DKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDM 490

Query: 498 DIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTLS 548
           D+G      +T YE+   WG KYF K NF+RLV VKT+VDP DFF  EQSIP ++
Sbjct: 491 DLGMYLGKKKTKYEEGKSWGVKYF-KNNFERLVRVKTRVDPTDFFCDEQSIPLVN 530

BLAST of Csor.00g297280 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 507.7 bits (1306), Expect = 1.2e-143
Identity = 249/517 (48.16%), Postives = 351/517 (67.89%), Query Frame = 0

Query: 36  HENFLQCLSHYSQDGSSISNVLYTPFNSSYSNVLNFTLRNLRFSTPHTPKPLLIITPLHV 95
           HENFL+CLSH   +    S +++T  + SY ++LN +++N RF    TPKP+ IITP+  
Sbjct: 34  HENFLKCLSHRINEDD--SRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSIITPVQA 93

Query: 96  SHIQAAVLCSKSHGLQIRTRSGGHDFEGLSYVA-SLPFIIIDLINLNSISIDVESKTAWV 155
           + +Q+ + C++ HG+ IRTRSGGHD+EGLSY+A S PF++IDL NL SI++DV+++T WV
Sbjct: 94  TDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVDNRTGWV 153

Query: 156 QSGTTLGELYYSIAKKSQTLTFPAGICPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQL 215
           QSG T+GELYY I K S++L FPAG+ PTVG+GG F GGGYG L+RKYGL+ADNVIDA +
Sbjct: 154 QSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADNVIDAHI 213

Query: 216 VDANGKFVNRKLMGEDLFWAIRGGGGGSFGIVVAWKVKLVSVPASVTLCMVNKTSEDGAT 275
           VDANG F++R+ MGED FWAIRGGGG SF +V++WK++L+ VP+ VT+  V KTSE  A 
Sbjct: 214 VDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKTSEKEAV 273

Query: 276 KLIHQWQYVAHKLENDIFVGILLGGGNFTRQGDKINPVALFFSLYLGKADELVAILNTTF 335
            +I++WQY+A K+ ND+F+  +L          +    A F  LYLG   +L+A++   F
Sbjct: 274 SIINKWQYIADKVPNDLFIRAML--------QKETEVYASFPGLYLGPVSDLLALMKDKF 333

Query: 336 PELGLSKKDCMETSWVETAIIMGTGLQLGLQPGSKLEDLLSKTTPIIASTKIKSDYVKEP 395
           PELGL   +C E SW+E+ +    G  +         ++L+K      S K K D+++EP
Sbjct: 334 PELGLEIGNCREMSWIESVLWFIKGESM---------EILAKRKRTSRSFKGKDDFIEEP 393

Query: 396 IPMAAIEGIWERLRSQDIELPQYIFVPYGGRMGQISESETPFSHRGEYMYKIGYVVGWVE 455
           IP  AI+ +W R  + +  L + I  P+GG+M +I+++E PF HR   +Y+I Y+  W E
Sbjct: 394 IPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAYWSE 453

Query: 456 QGIDAEK----RHIDWIRELYSYMTPFVSKSPRAAYLNYRDLDIGSNNKYG-RTSYEQAS 515
           +  D  K    +++ W+  +Y +MTP+VSKSPR AY+N+RD+D+G       +T YE+A 
Sbjct: 454 EE-DKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTKYEEAK 513

Query: 516 IWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTL 547
           +WG KYF K NFDRLV VKT VDP DFF  EQSIP +
Sbjct: 514 VWGVKYF-KNNFDRLVRVKTNVDPMDFFCDEQSIPIM 529

BLAST of Csor.00g297280 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 493.4 bits (1269), Expect = 2.3e-139
Identity = 249/544 (45.77%), Postives = 361/544 (66.36%), Query Frame = 0

Query: 6   MNSSSLLPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYT-PFN-S 65
           MN+++   ++L LL L    S++  +F    ++F+ CL   S     + +  +T   N S
Sbjct: 6   MNNTNAFLVTLLLLSL----SYIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNAS 65

Query: 66  SYSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEG 125
           S+   L  + +NLR+  P  PKP  I  PL+ +H+QAAVLC+K   L +R RSGGHD+EG
Sbjct: 66  SFKLALETSAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEG 125

Query: 126 LSYVASL--PFIIIDLINLNSISIDVESKTAWVQSGTTLGELYYSIAKKSQTLTFPAGIC 185
           LSYV+ +   F+I+DL  L  IS+D+ES +AWV +G ++GE+YY I +KS+   FPAG+C
Sbjct: 126 LSYVSEMETAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLC 185

Query: 186 PTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGGG 245
            ++G+GGH  GG YG ++RK+GL ADNV+DA++VDA+GK +NR  MGED+FWAIRGGGGG
Sbjct: 186 TSLGIGGHIIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGG 245

Query: 246 SFGIVVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFVGILLGGGN 305
           SFG+++AWK+KLV VP  VT+  V +T E   TKL+++WQ VA KL+ D+F+ +++   +
Sbjct: 246 SFGVILAWKIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTS 305

Query: 306 FTRQGDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGLQ 365
            T +  +      +   +LG A+ L+ ++  +FP+LGL+KKDC+ETSW+++ + +     
Sbjct: 306 KTPKSKERTISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIA---- 365

Query: 366 LGLQPGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFVP 425
            G    +  E LL   +      K KSDYV+EPIP+  +EG+WE+L  +D   P  I+ P
Sbjct: 366 -GFPSTAPSEALLDGKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEED--SPLTIWNP 425

Query: 426 YGGRMGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSKS 485
           YGG M +I E+ETPF HR   ++KI ++  W + G  +E +H+ W+RE+YSYM  +VSKS
Sbjct: 426 YGGMMAKIPETETPFPHRSGTLFKIQWLTLW-QDGKTSEAKHMGWMREMYSYMEQYVSKS 485

Query: 486 PRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQ 545
           PR+AY+NYRDLD+G N K        A  WG +YF K NF+RLV +K K DP +FFRHEQ
Sbjct: 486 PRSAYVNYRDLDLGMNGK-----GSDAREWGNRYF-KGNFERLVEIKAKFDPENFFRHEQ 531