Csor.00g297260 (gene) Silver-seed gourd (wild; sororia) v1

BLAST of Csor.00g297260 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 1.5e-151
Identity = 266/521 (51.06%), Postives = 355/521 (68.14%), Query Frame = 0

Query: 28  VSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSTYSSVLNITLRNLRFSTPHTPKPL 87
           + ++   P +NFL+C S Y  + ++   ++YT  N  Y SVLN T+ NLRF++  TPKPL
Sbjct: 23  IQTSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPL 82

Query: 88  LIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLSYVASLPFIIVDLINLNSISIDV 147
           +I+TP HVSHIQ  +LCSK  GLQIRTRSGGHD EG+SY++ +PF+IVDL N+ SI IDV
Sbjct: 83  VIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDV 142

Query: 148 ESKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTVGVGGHFSGGGYGMLLRKYGLAAD 207
            S+TAWV++GATLGE+YY + +K++ L+  AG CPTV  GGHF GGGYG L+R YGLAAD
Sbjct: 143 HSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAAD 202

Query: 208 NVIDAQLVDANGKFVDRKLMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPASVTLCMANK 267
           N+IDA LV+ +GK +DRK MGEDLFWA+RGGG  SFGI+VAWK++LV VP S    +   
Sbjct: 203 NIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPKSTMFSVKKI 262

Query: 268 TSEDGATKLIHQWQYVAHKLENNAFLGILLAGGNFTRQGGKINPLAL---FFSLYLGKAD 327
                  KL+++WQ +A+K + +  L       N T   GK N  A+   F S++LG  D
Sbjct: 263 MEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGK-NKTAIHTYFSSVFLGGVD 322

Query: 328 ELLTILNTTFPELGLSKKDCLETSWVETALIMGTGLQFGS---KLEDLLSKATLVNGSTK 387
            L+ ++N +FPELG+ K DC + SW++T +     + + +     E LL ++   NG+ K
Sbjct: 323 SLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNKEILLDRSAGQNGAFK 382

Query: 388 IKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQIPEFETPFSHRGEYLYK 447
           IK DY+K+PIP +    I E+L  EDI    +   PYGG M +I E   PF HR   LY+
Sbjct: 383 IKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIPFPHRAGILYE 442

Query: 448 IGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLNYRDLDIGSNNKYGRTSY 507
           + YI +W +Q  E  ++H++WIR +Y++MTP+VSK+PR AYLNYRDLDIG N+     +Y
Sbjct: 443 LWYICSWEKQ--EDNEKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGINDPKNPNNY 502

Query: 508 EQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTL 543
            QA IWG KYFGK NFDRLV VKT VDP +FFR+EQSIP L
Sbjct: 503 TQARIWGEKYFGK-NFDRLVKVKTLVDPNNFFRNEQSIPPL 539

BLAST of Csor.00g297260 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 2.5e-151
Identity = 264/521 (50.67%), Postives = 357/521 (68.52%), Query Frame = 0

Query: 28  VSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSTYSSVLNITLRNLRFSTPHTPKPL 87
           +  +   P +NFL+C S Y     + + ++YT  +  Y S+LN T++NLRF++  TPKPL
Sbjct: 23  IQISIANPQENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPL 82

Query: 88  LIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLSYVASLPFIIVDLINLNSISIDV 147
           +IITPL+VSHIQ  +LCSK  GLQIRTRSGGHD EG+SY++ +PF+IVDL N++S+ IDV
Sbjct: 83  VIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDV 142

Query: 148 ESKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTVGVGGHFSGGGYGMLLRKYGLAAD 207
            S+TAWV++GATLGE+YY I + ++ L+FPAG CPTVG GGHFSGGGYG L+R YGLAAD
Sbjct: 143 HSQTAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAAD 202

Query: 208 NVIDAQLVDANGKFVDRKLMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPASVTLCMANK 267
           N+IDA LV+ +GK +DRK MGEDLFWAIRGGGG +FGI+ AWK++LV VP+  T+    K
Sbjct: 203 NIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKK 262

Query: 268 TSE-DGATKLIHQWQYVAHKLENNAFLGILLAGGNFTRQGGKINPL--ALFFSLYLGKAD 327
             E     KL+++WQ +A+  E    L       N T   GK      + F S++ G  D
Sbjct: 263 NMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVD 322

Query: 328 ELLTILNTTFPELGLSKKDCLETSWVETALIMGTGLQFGS---KLEDLLSKATLVNGSTK 387
            L+ ++N +FPELG+ K DC + SW++T +     + + +   K E LL ++     +  
Sbjct: 323 SLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFS 382

Query: 388 IKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQIPEFETPFSHRGEYLYK 447
           IK DY+K+PIP  A+  I E+L  ED+ +   +F PYGG M +I E   PF HR   +Y+
Sbjct: 383 IKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYE 442

Query: 448 IGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLNYRDLDIGSNNKYGRTSY 507
           I YI +W +Q  E  ++HI+WIR +Y++ TP+VS++PR AYLNYRDLD+G  N     +Y
Sbjct: 443 IWYIASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNY 502

Query: 508 EQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTL 543
            QA IWG KYFGK NF+RLV VKTKVDP +FFR+EQSIP L
Sbjct: 503 TQARIWGEKYFGK-NFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Csor.00g297260 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 4.3e-151
Identity = 265/521 (50.86%), Postives = 356/521 (68.33%), Query Frame = 0

Query: 28  VSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSTYSSVLNITLRNLRFSTPHTPKPL 87
           +  +   P +NFL+CLS Y     + + ++YT  +  Y S+LN T++NLRF++  TPKPL
Sbjct: 23  IQISIANPQENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPL 82

Query: 88  LIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLSYVASLPFIIVDLINLNSISIDV 147
           +I TPL+VSHIQ  +LCSK  GLQIRTRSGGHD EG+SY++ +PF+IVDL N++S+ IDV
Sbjct: 83  VITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDV 142

Query: 148 ESKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTVGVGGHFSGGGYGMLLRKYGLAAD 207
            S+TAWV+SGATLGE+YY I + ++ L+FPAG CPTVG GGHFSGGGYG L+R YGLAAD
Sbjct: 143 HSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAAD 202

Query: 208 NVIDAQLVDANGKFVDRKLMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPASVTLCMANK 267
           N+IDA LV+ +GK +DRK MGEDLFWAIRGGGG +FGI+ AWK++LV VP+  T+    K
Sbjct: 203 NIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKK 262

Query: 268 TSE-DGATKLIHQWQYVAHKLENNAFLGILLAGGNFTRQGGKINPL--ALFFSLYLGKAD 327
             E     KL+++WQ +A+  E    L       N T   GK      + F S++ G  D
Sbjct: 263 NMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVD 322

Query: 328 ELLTILNTTFPELGLSKKDCLETSWVETALIMGTGLQFGS---KLEDLLSKATLVNGSTK 387
            L+ ++N +FPELG+ K DC + SW++T +     + + +   K E LL ++     +  
Sbjct: 323 SLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDRSGGRKAAFS 382

Query: 388 IKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQIPEFETPFSHRGEYLYK 447
           IK DY+K+PIP  A+  I E+L  ED+ +   +F PYGG M +I E   PF HR    Y+
Sbjct: 383 IKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYE 442

Query: 448 IGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLNYRDLDIGSNNKYGRTSY 507
           I YI +W +Q  E  ++HI+WIR +Y++ TP+VS++PR AYLNYRDLD+G  N     +Y
Sbjct: 443 IWYIASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNY 502

Query: 508 EQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTL 543
            QA IWG KYFGK NF+RLV VKTKVDP +FFR+EQSIP L
Sbjct: 503 TQARIWGEKYFGK-NFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Csor.00g297260 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 8.2e-150
Identity = 266/543 (48.99%), Postives = 363/543 (66.85%), Query Frame = 0

Query: 6   MNSSSLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSTY 65
           MN S+     +  ++    S  +  +   P +NFL+C S +  +  +   ++YT  +  Y
Sbjct: 1   MNCSAFSFWFVCKIIFFFLSFHIQISIANPRENFLKCFSKHIPNNVANPKLVYTQHDQLY 60

Query: 66  SSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLS 125
            S+LN T++NLRF +  TPKPL+I+TP + SHIQA +LCSK  GLQIRTRSGGHD EG+S
Sbjct: 61  MSILNSTIQNLRFISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMS 120

Query: 126 YVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTVG 185
           Y++ +PF++VDL N++SI IDV S+TAWV++GATLGE+YY I +K++ L+FP G CPTVG
Sbjct: 121 YISQVPFVVVDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVG 180

Query: 186 VGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVDRKLMGEDLFWAIRGGGGGSFGI 245
           VGGHFSGGGYG L+R YGLAADN+IDA LV+ +GK +DRK MGEDLFWAIRGGGG +FGI
Sbjct: 181 VGGHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGI 240

Query: 246 VVAWKVKLVPVPASVTLCMANKTSE-DGATKLIHQWQYVAHKLENNAFLGILLAGGNFTR 305
           + AWK+KLV VP+  T+    K  E  G  KL ++WQ +A+K + +  L       N T 
Sbjct: 241 IAAWKIKLVAVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITD 300

Query: 306 QGGKINPL--ALFFSLYLGKADELLTILNTTFPELGLSKKDCLETSWVETALIMGTGLQF 365
             GK        F S++ G  D L+ ++N +FPELG+ K DC E SW++T +     + F
Sbjct: 301 NHGKNKTTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNF 360

Query: 366 GS---KLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYG 425
            +   K E LL ++     +  IK DY+K+PIP  A+  I E+L  ED+    ++  PYG
Sbjct: 361 NTANFKKEILLDRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYG 420

Query: 426 GKMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPR 485
           G M +I E   PF HR   +Y++ Y  +W +Q  E  ++HI+W+R +Y++ TP+VS++PR
Sbjct: 421 GIMEEISESAIPFPHRAGIMYELWYTASWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPR 480

Query: 486 AAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSI 543
            AYLNYRDLD+G  N     +Y QA IWG KYFGK NF+RLV VKTKVDP +FFR+EQSI
Sbjct: 481 LAYLNYRDLDLGKTNHASPNNYTQARIWGEKYFGK-NFNRLVKVKTKVDPNNFFRNEQSI 540

BLAST of Csor.00g297260 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 9.1e-149
Identity = 262/543 (48.25%), Postives = 361/543 (66.48%), Query Frame = 0

Query: 6   MNSSSLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSTY 65
           MN S+     +  ++    S  +  +   P +NFL+C S Y  +  +    +YT  +  Y
Sbjct: 1   MNCSAFSFWFVCKIIFFFLSFNIQISIANPQENFLKCFSEYIPNNPANPKFIYTQHDQLY 60

Query: 66  SSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLS 125
            SVLN T++NLRF++  TPKPL+I+TP +VSHIQA++LCSK  GLQIRTRSGGHD EGLS
Sbjct: 61  MSVLNSTIQNLRFTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLS 120

Query: 126 YVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTVG 185
           Y++ +PF IVDL N++++ +D+ S+TAWV++GATLGE+YY I + ++  +FP G CPTVG
Sbjct: 121 YISQVPFAIVDLRNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVG 180

Query: 186 VGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVDRKLMGEDLFWAIRGGGGGSFGI 245
           VGGHFSGGGYG L+R YGLAADN+IDA LV+ +GK +DRK MGEDLFWAIRGGGG +FGI
Sbjct: 181 VGGHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGI 240

Query: 246 VVAWKVKLVPVPASVTLCMANKTSE-DGATKLIHQWQYVAHKLENNAFLGILLAGGNFTR 305
           + AWK+KLV VP+  T+    K  E  G  KL ++WQ +A+K + +  L       N T 
Sbjct: 241 IAAWKIKLVVVPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITD 300

Query: 306 QGGKINPL--ALFFSLYLGKADELLTILNTTFPELGLSKKDCLETSWVETALIMGTGLQF 365
             GK        F S++LG  D L+ ++N +FPELG+ K DC E SW++T +     + +
Sbjct: 301 NHGKNKTTVHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNY 360

Query: 366 GS---KLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYG 425
            +   K E LL ++     +  IK DY+K+ IP  A+  I E+L  E++ +  ++  PYG
Sbjct: 361 NTANFKKEILLDRSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYG 420

Query: 426 GKMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPR 485
           G M +I E   PF HR   +Y++ Y   W +Q  E  ++HI+W+R +Y++ TP+VS++PR
Sbjct: 421 GIMDEISESAIPFPHRAGIMYELWYTATWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPR 480

Query: 486 AAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSI 543
            AYLNYRDLD+G  N     +Y QA IWG KYFGK NF+RLV VKTK DP +FFR+EQSI
Sbjct: 481 LAYLNYRDLDLGKTNPESPNNYTQARIWGEKYFGK-NFNRLVKVKTKADPNNFFRNEQSI 540

BLAST of Csor.00g297260 vs. NCBI nr
Match: KAG6573426.1 (Cannabidiolic acid synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1103 bits (2852), Expect = 0.0
Identity = 545/545 (100.00%), Postives = 545/545 (100.00%), Query Frame = 0

Query: 1   MSNSAMNSSSLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60
           MSNSAMNSSSLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP
Sbjct: 1   MSNSAMNSSSLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60

Query: 61  FNSTYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNSTYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSTYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180
           FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVDRKLMGEDLFWAIRGGGG 240
           CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVDRKLMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVDRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300
           GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG
Sbjct: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300

Query: 301 NFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCLETSWVETALIMGTGL 360
           NFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCLETSWVETALIMGTGL
Sbjct: 301 NFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCLETSWVETALIMGTGL 360

Query: 361 QFGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420
           QFGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG
Sbjct: 361 QFGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420

Query: 421 KMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480
           KMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA
Sbjct: 421 KMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480

Query: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540
           AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540

Query: 541 TLSSK 545
           TLSSK
Sbjct: 541 TLSSK 545

BLAST of Csor.00g297260 vs. NCBI nr
Match: XP_022994835.1 (berberine bridge enzyme-like 18 [Cucurbita maxima])

HSP 1 Score: 1088 bits (2815), Expect = 0.0
Identity = 537/545 (98.53%), Postives = 541/545 (99.27%), Query Frame = 0

Query: 1   MSNSAMNSSSLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60
           MSNSAMNSS LLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60

Query: 61  FNSTYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNS+YSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180
           FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYY IAKKS+TLAFPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAKKSRTLAFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVDRKLMGEDLFWAIRGGGG 240
           CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFV+RKLMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300
           GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG
Sbjct: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300

Query: 301 NFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCLETSWVETALIMGTGL 360
           NFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDC+ETSWVETALIMGTGL
Sbjct: 301 NFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL 360

Query: 361 QFGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420
           Q GSKLEDLLSKA LVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG
Sbjct: 361 QLGSKLEDLLSKAPLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420

Query: 421 KMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480
           KMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA
Sbjct: 421 KMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480

Query: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540
           AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540

Query: 541 TLSSK 545
           TLSSK
Sbjct: 541 TLSSK 545

BLAST of Csor.00g297260 vs. NCBI nr
Match: XP_023542525.1 (berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1087 bits (2810), Expect = 0.0
Identity = 538/546 (98.53%), Postives = 543/546 (99.45%), Query Frame = 0

Query: 1   MSNSAMNSSSLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60
           MSNSAMNSS LLPLSLTLLVLCSCSSWVS+NFVKPHQ+FLQCLSHYSQD SSISDVLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSANFVKPHQSFLQCLSHYSQDASSISDVLYTP 60

Query: 61  FNSTYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNS+YSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180
           FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVDRK-LMGEDLFWAIRGGG 240
           CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFV+RK LMGEDLFWAIRGGG
Sbjct: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRKKLMGEDLFWAIRGGG 240

Query: 241 GGSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAG 300
           GGSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAG
Sbjct: 241 GGSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAG 300

Query: 301 GNFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCLETSWVETALIMGTG 360
           GNFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDC+ETSWVETALIMGTG
Sbjct: 301 GNFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCMETSWVETALIMGTG 360

Query: 361 LQFGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYG 420
           LQFGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYG
Sbjct: 361 LQFGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYG 420

Query: 421 GKMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPR 480
           GKMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPR
Sbjct: 421 GKMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPR 480

Query: 481 AAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSI 540
           AAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSI
Sbjct: 481 AAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSI 540

Query: 541 PTLSSK 545
           PTLSSK
Sbjct: 541 PTLSSK 546

BLAST of Csor.00g297260 vs. NCBI nr
Match: XP_022954543.1 (berberine bridge enzyme-like 18 [Cucurbita moschata])

HSP 1 Score: 1086 bits (2809), Expect = 0.0
Identity = 536/545 (98.35%), Postives = 542/545 (99.45%), Query Frame = 0

Query: 1   MSNSAMNSSSLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60
           MSNSAMNSS LLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60

Query: 61  FNSTYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNS+YSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180
           FEGLSYVASLPFIIVDLINLNSISIDVE+KTAWVQSGATLGELYYSIAKKSQTLAFPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVDRKLMGEDLFWAIRGGGG 240
           CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFV+R+LMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300
           GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG
Sbjct: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300

Query: 301 NFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCLETSWVETALIMGTGL 360
           NFTRQGGKINPLALFFSLYLGKA+ELLTILNTTFPELGLSKKDC+ETSWVETALIMGTGL
Sbjct: 301 NFTRQGGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL 360

Query: 361 QFGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420
           Q GSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG
Sbjct: 361 QLGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420

Query: 421 KMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480
           KMGQIPEFETPFSHRGE LYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA
Sbjct: 421 KMGQIPEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480

Query: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540
           AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540

Query: 541 TLSSK 545
           TLSSK
Sbjct: 541 TLSSK 545

BLAST of Csor.00g297260 vs. NCBI nr
Match: XP_023542145.1 (LOW QUALITY PROTEIN: berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1032 bits (2669), Expect = 0.0
Identity = 506/545 (92.84%), Postives = 524/545 (96.15%), Query Frame = 0

Query: 1   MSNSAMNSSSLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60
           MSN AMNSS LLPLSLTLLVLCSCSSWVS+NF KPH+NFLQCLSHYSQD SSISDVLYTP
Sbjct: 1   MSNLAMNSSPLLPLSLTLLVLCSCSSWVSANFAKPHENFLQCLSHYSQDASSISDVLYTP 60

Query: 61  FNSTYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNS+YS+VLN TLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180
           FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYY IA+KS+TLAFPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRTLAFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVDRKLMGEDLFWAIRGGGG 240
           CPTVGVGGH SGGG+GMLLRKYGLAADNVIDAQLVDANGKFV+RKLMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHLSGGGFGMLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300
           GSFGIVVAWKVKLVPVPASVTLCMANKT EDGATKLIHQWQYVAHKLE++ FLGIL+ GG
Sbjct: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTLEDGATKLIHQWQYVAHKLESDVFLGILVGGG 300

Query: 301 NFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCLETSWVETALIMGTGL 360
            F RQGGKINPLALFFSLYLGKADELLTILNTTFPELG+SKKDC E SWVETALIM TG 
Sbjct: 301 KFMRQGGKINPLALFFSLYLGKADELLTILNTTFPELGMSKKDCKEMSWVETALIMATGR 360

Query: 361 QFGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420
           Q GSKLEDLLSK T VNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIE+PQHIFVPYGG
Sbjct: 361 QLGSKLEDLLSKETPVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEIPQHIFVPYGG 420

Query: 421 KMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480
           KMGQIPEFE+P+SHRGEYLYKIGY VNWAEQGI+AEKRH+DWIREL+SYMTPFVSKSPRA
Sbjct: 421 KMGQIPEFESPYSHRGEYLYKIGYGVNWAEQGIDAEKRHMDWIRELHSYMTPFVSKSPRA 480

Query: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540
            YLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 TYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540

Query: 541 TLSSK 545
           TLSSK
Sbjct: 541 TLSSK 545

BLAST of Csor.00g297260 vs. ExPASy TrEMBL
Match: A0A6J1K2F5 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111490442 PE=3 SV=1)

HSP 1 Score: 1088 bits (2815), Expect = 0.0
Identity = 537/545 (98.53%), Postives = 541/545 (99.27%), Query Frame = 0

Query: 1   MSNSAMNSSSLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60
           MSNSAMNSS LLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60

Query: 61  FNSTYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNS+YSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180
           FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYY IAKKS+TLAFPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAKKSRTLAFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVDRKLMGEDLFWAIRGGGG 240
           CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFV+RKLMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300
           GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG
Sbjct: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300

Query: 301 NFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCLETSWVETALIMGTGL 360
           NFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDC+ETSWVETALIMGTGL
Sbjct: 301 NFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL 360

Query: 361 QFGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420
           Q GSKLEDLLSKA LVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG
Sbjct: 361 QLGSKLEDLLSKAPLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420

Query: 421 KMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480
           KMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA
Sbjct: 421 KMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480

Query: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540
           AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540

Query: 541 TLSSK 545
           TLSSK
Sbjct: 541 TLSSK 545

BLAST of Csor.00g297260 vs. ExPASy TrEMBL
Match: A0A6J1GRE1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456783 PE=3 SV=1)

HSP 1 Score: 1086 bits (2809), Expect = 0.0
Identity = 536/545 (98.35%), Postives = 542/545 (99.45%), Query Frame = 0

Query: 1   MSNSAMNSSSLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60
           MSNSAMNSS LLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60

Query: 61  FNSTYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNS+YSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180
           FEGLSYVASLPFIIVDLINLNSISIDVE+KTAWVQSGATLGELYYSIAKKSQTLAFPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVDRKLMGEDLFWAIRGGGG 240
           CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFV+R+LMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300
           GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG
Sbjct: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300

Query: 301 NFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCLETSWVETALIMGTGL 360
           NFTRQGGKINPLALFFSLYLGKA+ELLTILNTTFPELGLSKKDC+ETSWVETALIMGTGL
Sbjct: 301 NFTRQGGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGL 360

Query: 361 QFGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420
           Q GSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG
Sbjct: 361 QLGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420

Query: 421 KMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480
           KMGQIPEFETPFSHRGE LYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA
Sbjct: 421 KMGQIPEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480

Query: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540
           AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540

Query: 541 TLSSK 545
           TLSSK
Sbjct: 541 TLSSK 545

BLAST of Csor.00g297260 vs. ExPASy TrEMBL
Match: A0A6J1K3Z1 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111490441 PE=3 SV=1)

HSP 1 Score: 1023 bits (2646), Expect = 0.0
Identity = 504/549 (91.80%), Postives = 524/549 (95.45%), Query Frame = 0

Query: 1   MSNSAMNSSSLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60
           MSNSAMNSS LLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60

Query: 61  FNSTYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNS+YSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180
           FEGLSYVASLPFIIVDLI LNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLIKLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVDRKLMGEDLFWAIRGGGG 240
           CPTVGVGGHFSGGGYG LLRKYGLAADNVIDAQLVDANGKFV+R+LMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRELMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300
           GSFGIVVAWKV LV VPASVTLCMA+KTSEDGATKLIHQWQYVAHKLEN+ FLGILL GG
Sbjct: 241 GSFGIVVAWKVNLVSVPASVTLCMASKTSEDGATKLIHQWQYVAHKLENDIFLGILLGGG 300

Query: 301 NFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCLETSWVETALIMGTGL 360
           NF+RQG KINP+ALFFSLYLGKADEL+ IL+T FPELGLSKKDC+ETSWVETA+IMGTGL
Sbjct: 301 NFSRQGDKINPVALFFSLYLGKADELVAILDTKFPELGLSKKDCMETSWVETAIIMGTGL 360

Query: 361 QFG----SKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFV 420
           Q G    SKLEDL SK T +N STKIKSDY+KEPIPMAAIEGIWERLR +DIE+PQH+FV
Sbjct: 361 QPGLQPGSKLEDLQSKTTPINASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQHLFV 420

Query: 421 PYGGKMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSK 480
           PYGG+MGQI EFETPFSHRGEY+YKIGY V W E GI+AEKRHIDWIRE+YSYMTPFVSK
Sbjct: 421 PYGGRMGQIQEFETPFSHRGEYMYKIGYTVGWVEGGIDAEKRHIDWIREIYSYMTPFVSK 480

Query: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 540
           SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE
Sbjct: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 540

Query: 541 QSIPTLSSK 545
           QSIPTLSSK
Sbjct: 541 QSIPTLSSK 549

BLAST of Csor.00g297260 vs. ExPASy TrEMBL
Match: A0A6J1GRG1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456798 PE=3 SV=1)

HSP 1 Score: 1021 bits (2641), Expect = 0.0
Identity = 502/549 (91.44%), Postives = 523/549 (95.26%), Query Frame = 0

Query: 1   MSNSAMNSSSLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60
           MSNSAMNSS LLPLSLTLLVLCSCSSWVS+NF KPH+NFLQCLSHYSQDGSSIS+VLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTP 60

Query: 61  FNSTYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNS+YS+VLN TLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180
           FEGLSYVASLPFIIVDLINLNSISIDV+SKTAWVQSGATLGELYYSIAKKSQTL FPAGI
Sbjct: 121 FEGLSYVASLPFIIVDLINLNSISIDVKSKTAWVQSGATLGELYYSIAKKSQTLTFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVDRKLMGEDLFWAIRGGGG 240
           CPTVGVGGHFSGGGYG LLRKYGLAADNVIDAQLVDANGKFV+RKLMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300
           GSFGIVVAWKVKLV VPASVTLCM NKTSEDGATKLIHQWQYVAHKLEN+ FLGILL GG
Sbjct: 241 GSFGIVVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFLGILLGGG 300

Query: 301 NFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCLETSWVETALIMGTGL 360
           NFTRQG KINP+ALFFSLYLGKADEL+ ILNTTFPELGLSKKDC+ETSWVETA+IMGTGL
Sbjct: 301 NFTRQGDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGL 360

Query: 361 QFG----SKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFV 420
           Q G    SKLEDLLSK T +  STKIKSDY+KEPIPMAAIEGIWERLR +DIE+PQ+IFV
Sbjct: 361 QLGLQPGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFV 420

Query: 421 PYGGKMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSK 480
           PYGG+MGQI E ETPFSHRGEY+YKIGY+V W EQGI+AEKRHIDWIRELYSYMTPFVSK
Sbjct: 421 PYGGRMGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSK 480

Query: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHE 540
           SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFG ENFDRLVHVKTKVDPYDFFRHE
Sbjct: 481 SPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGNENFDRLVHVKTKVDPYDFFRHE 540

Query: 541 QSIPTLSSK 545
           QSIPTLSSK
Sbjct: 541 QSIPTLSSK 549

BLAST of Csor.00g297260 vs. ExPASy TrEMBL
Match: A0A6J1GRE5 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456787 PE=3 SV=1)

HSP 1 Score: 986 bits (2548), Expect = 0.0
Identity = 484/545 (88.81%), Postives = 506/545 (92.84%), Query Frame = 0

Query: 1   MSNSAMNSSSLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTP 60
           MSNSAMNSS LLPLSLTLLVLCSCSSWVSSNF KPH+NFLQCLSHYSQD SSIS+VLYTP
Sbjct: 1   MSNSAMNSSPLLPLSLTLLVLCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTP 60

Query: 61  FNSTYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120
           FNS+YS VLN TLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD
Sbjct: 61  FNSSYSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHD 120

Query: 121 FEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGI 180
            EGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYY IA+KS++LAFPAGI
Sbjct: 121 IEGLSYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGI 180

Query: 181 CPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVDRKLMGEDLFWAIRGGGG 240
           CPTVGVGGH SGGGYG+LLRKYGLAADNVIDAQLVDANGKF DRKLMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGG 300
           GSFGIVVAWKV LV VPA+VTLC+ NKT EDGATKLIHQWQYVAHKLENN FLGILL GG
Sbjct: 241 GSFGIVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLGILLEGG 300

Query: 301 NFTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCLETSWVETALIMGTGL 360
           NFTRQG KINPLALFFS YLGKADELLTILNTTFPEL LSKKDC+ETSWVETALIMGTGL
Sbjct: 301 NFTRQGSKINPLALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGTGL 360

Query: 361 QFGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGG 420
           Q GSKLEDLLSK  L+  STK+KSDY+KEPIPMAAIEGI +RLR  DIE PQ IF+PYGG
Sbjct: 361 QPGSKLEDLLSKTPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGG 420

Query: 421 KMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRA 480
           KM +I E ETPFSHRGEY YKIGY  +W E+GI+AE +H++WIRELYSYM PFVSKSPRA
Sbjct: 421 KMSEISELETPFSHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPRA 480

Query: 481 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540
           AYLNYRDLDIGSNNKYGRTSYEQA IWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 AYLNYRDLDIGSNNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 540

Query: 541 TLSSK 545
           T SSK
Sbjct: 541 TFSSK 545

BLAST of Csor.00g297260 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 526.2 bits (1354), Expect = 3.2e-149
Identity = 264/532 (49.62%), Postives = 368/532 (69.17%), Query Frame = 0

Query: 9   SSLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSTYSSV 68
           SS+L    T  +L S    VS+N      +FLQCLS    D + +S V++TP ++++SSV
Sbjct: 8   SSVLIFFTTSTLLLSIPHPVSAN-RSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSV 67

Query: 69  LNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLSYVA 128
           L  +++N RFS P  PKP+LI+TP+  S +Q+AV C++  G+ IRTRSGGHD+EGLSYV 
Sbjct: 68  LASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVT 127

Query: 129 SLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTVGVGG 188
             PF+I+DL NL SI++DV++++ WVQ+GAT+GELYY I KK++TLAFPAG+CPTVGVGG
Sbjct: 128 HKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGG 187

Query: 189 HFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVDRKLMGEDLFWAIRGGGGGSFGIVVA 248
           HFSGGGYG LLRK+GLAAD+VIDA++VDA G+ ++R+ MGED FWAIRGGGG SF +V++
Sbjct: 188 HFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLS 247

Query: 249 WKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGGNFTRQGGK 308
           WK+ L+ VP++VT+    K SE  A K+IH+WQ+VA K+ ++ F+ ++L       Q  K
Sbjct: 248 WKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML-------QRYK 307

Query: 309 INPLALFFSLYLGKADELLTILNTTFPELGLSKKDCLETSWVETALIMGTGLQFGSKLED 368
               A F  LYLG    LL ++N  FPELGL + DC E SW+E+ +      + G +  +
Sbjct: 308 NMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA---ELGEEPIN 367

Query: 369 LLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQIPEF 428
           +L+K T  + + K KSD+++EP+P  AI  +W RL+  + E  Q IF P+GGKM +I ++
Sbjct: 368 VLTKRTRASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADY 427

Query: 429 ETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLNYRDL 488
           ETPF HR   +Y+I Y+  W     + +++++ W+  +Y  M+ FV+KSPR AY+N RDL
Sbjct: 428 ETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDL 487

Query: 489 DIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIP 541
           D+G      R+ YE+   WG KYF K NF+RLV VKT VDP DFF  EQSIP
Sbjct: 488 DLGMYVGVKRSKYEEGKSWGVKYF-KNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of Csor.00g297260 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 515.0 bits (1325), Expect = 7.4e-146
Identity = 264/531 (49.72%), Postives = 359/531 (67.61%), Query Frame = 0

Query: 15  SLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSTYSSVLNITLR 74
           +L L++      W SS+     + F QCL+  S     IS  ++   N +YSSVL   +R
Sbjct: 4   ALILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIR 63

Query: 75  NLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLSYV--ASLPF 134
           NLRF+T  TPKP LII   H SH+QAA+ C K H LQ++ RSGGHD++GLSYV  +  PF
Sbjct: 64  NLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPF 123

Query: 135 IIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTVGVGGHFSG 194
            ++D+ NL S+ +DV SKTAWVQ+GA LGE+YY I +KS+TLA+PAGICPTVGVGGH SG
Sbjct: 124 FVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISG 183

Query: 195 GGYGMLLRKYGLAADNVIDAQLVDANGKFVDRKLMGEDLFWAIRGGGGGSFGIVVAWKVK 254
           GGYG ++RKYGL  DN IDA++VD NGK +DRKLMGEDL+WAI GGGGGS+G+V+A+K+ 
Sbjct: 184 GGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKIN 243

Query: 255 LVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGGNFTRQGGKINPL 314
           LV VP +VT+   ++T E  AT +IH+WQ VA KL +  F+  ++   N T    K    
Sbjct: 244 LVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQK-TVR 303

Query: 315 ALFFSLYLGKADELLTILNTTFPELGLSKKDCLETSWVETALIMGTGLQFGSKLEDLLSK 374
             F +++LG    LL+ILN  FPELGL + DC ETSW+++ L   T +Q GS    LL +
Sbjct: 304 TTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFW-TNIQVGSSETLLLQR 363

Query: 375 ATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQIPEFETPF 434
              VN   K KSDY++EPI    +E IW+++   ++E+P   F PYGG+MG+I    TPF
Sbjct: 364 NQPVN-YLKRKSDYVREPISRTGLESIWKKM--IELEIPTMAFNPYGGEMGRISSTVTPF 423

Query: 435 SHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLNYRDLDIGS 494
            +R   L+KI Y  NW ++ +    R+++  R+LY +MTPFVSK+PR ++ NYRD+D+G 
Sbjct: 424 PYRAGNLWKIQYGANWRDETL--TDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGI 483

Query: 495 NNKYGR-TSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTL 543
           N+  G+ +SY +   +G KYF   NF+RLV +KT+VD  +FFR+EQSIP L
Sbjct: 484 NSHNGKISSYVEGKRYGKKYFA-GNFERLVKIKTRVDSGNFFRNEQSIPVL 526

BLAST of Csor.00g297260 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 508.8 bits (1309), Expect = 5.3e-144
Identity = 250/512 (48.83%), Postives = 352/512 (68.75%), Query Frame = 0

Query: 36  HQNFLQCLSHYSQDGSSISDVLYTPFNSTYSSVLNITLRNLRFSTPHTPKPLLIITPLHV 95
           H++FL+CLS+   D +    V++T  +S++ S+L+ +++N RFS   TPKP+ IITP+  
Sbjct: 28  HEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSIQNPRFSVSETPKPVSIITPVKA 87

Query: 96  SHIQAAVLCSKSHGLQIRTRSGGHDFEGLSYVA-SLPFIIVDLINLNSISIDVESKTAWV 155
           S +Q  + C++ HG+ +RTRS GH +EGLSY+A + PF ++DL NL SIS+DV+++T WV
Sbjct: 88  SDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVIDLRNLRSISLDVDNRTGWV 147

Query: 156 QSGATLGELYYSIAKKSQTLAFPAGICPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQL 215
           Q+GAT GELYY I K +++LAFPAGI PTVGVGG FSGGGYG LLRKYGLAADN+IDA +
Sbjct: 148 QTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYGTLLRKYGLAADNIIDALV 207

Query: 216 VDANGKFVDRKLMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPASVTLCMANKTSEDGAT 275
           VDA+G+ +DR+ MGED FWAIRGGGG SFG++++WKVKLV VP+++T+    KTS+  A 
Sbjct: 208 VDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVPSTITVFKVQKTSKKEAV 267

Query: 276 KLIHQWQYVAHKLENNAFLGILLAGGNFTRQGGKINPLALFFSLYLGKADELLTILNTTF 335
           ++I +WQY A K+ ++ F+   L   N      K    ALF  LY+G  + LL ++   F
Sbjct: 268 RIIKKWQYAADKVPDDLFIRTTLERSN------KNAVHALFTGLYIGPVNNLLALMEEKF 327

Query: 336 PELGLSKKDCLETSWVETALIMGTGLQFGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMA 395
           PELGL K+ C E SW+E+ L        G  L  +L+     + S K K D+++EPIP A
Sbjct: 328 PELGLEKEGCEEMSWIESVLWF-ADFPKGESL-GVLTNRERTSLSFKGKDDFVQEPIPEA 387

Query: 396 AIEGIWERLRYEDIEMPQHIFVPYGGKMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIE 455
           AI+ IW RL   +  + + I  P+GGKM ++ E+ETPF HRG  LY+I Y+  W E+  +
Sbjct: 388 AIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHRGGNLYEIQYVAYWREEEDK 447

Query: 456 AE---KRHIDWIRELYSYMTPFVSKSPRAAYLNYRDLDIGSNNKYGRTSYEQASIWGYKY 515
            +    +++ W+  +Y +MTP+VSKSPR AY+N++D+D+G      +T YE+   WG KY
Sbjct: 448 NKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGMYLGKKKTKYEEGKSWGVKY 507

Query: 516 FGKENFDRLVHVKTKVDPYDFFRHEQSIPTLS 544
           F K NF+RLV VKT+VDP DFF  EQSIP ++
Sbjct: 508 F-KNNFERLVRVKTRVDPTDFFCDEQSIPLVN 530

BLAST of Csor.00g297260 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 506.1 bits (1302), Expect = 3.4e-143
Identity = 249/512 (48.63%), Postives = 350/512 (68.36%), Query Frame = 0

Query: 36  HQNFLQCLSHYSQDGSSISDVLYTPFNSTYSSVLNITLRNLRFSTPHTPKPLLIITPLHV 95
           H+NFL+CLSH   +    S +++T  + +Y S+LN +++N RF    TPKP+ IITP+  
Sbjct: 34  HENFLKCLSHRINEDD--SRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSIITPVQA 93

Query: 96  SHIQAAVLCSKSHGLQIRTRSGGHDFEGLSYVA-SLPFIIVDLINLNSISIDVESKTAWV 155
           + +Q+ + C++ HG+ IRTRSGGHD+EGLSY+A S PF+++DL NL SI++DV+++T WV
Sbjct: 94  TDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVDNRTGWV 153

Query: 156 QSGATLGELYYSIAKKSQTLAFPAGICPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQL 215
           QSGAT+GELYY I K S++LAFPAG+ PTVG+GG F GGGYG L+RKYGL+ADNVIDA +
Sbjct: 154 QSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADNVIDAHI 213

Query: 216 VDANGKFVDRKLMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPASVTLCMANKTSEDGAT 275
           VDANG F+DR+ MGED FWAIRGGGG SF +V++WK++L+ VP+ VT+    KTSE  A 
Sbjct: 214 VDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKTSEKEAV 273

Query: 276 KLIHQWQYVAHKLENNAFLGILLAGGNFTRQGGKINPLALFFSLYLGKADELLTILNTTF 335
            +I++WQY+A K+ N+ F+  +L          +    A F  LYLG   +LL ++   F
Sbjct: 274 SIINKWQYIADKVPNDLFIRAMLQ--------KETEVYASFPGLYLGPVSDLLALMKDKF 333

Query: 336 PELGLSKKDCLETSWVETALIMGTGLQFGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMA 395
           PELGL   +C E SW+E+ L    G     +  ++L+K    + S K K D+I+EPIP  
Sbjct: 334 PELGLEIGNCREMSWIESVLWFIKG-----ESMEILAKRKRTSRSFKGKDDFIEEPIPKT 393

Query: 396 AIEGIWERLRYEDIEMPQHIFVPYGGKMGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIE 455
           AI+ +W R    +  + + I  P+GGKM +I + E PF HR   LY+I Y+  W+E+  +
Sbjct: 394 AIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAYWSEEEDK 453

Query: 456 AE---KRHIDWIRELYSYMTPFVSKSPRAAYLNYRDLDIGSNNKYG-RTSYEQASIWGYK 515
            +   ++++ W+  +Y +MTP+VSKSPR AY+N+RD+D+G       +T YE+A +WG K
Sbjct: 454 NKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTKYEEAKVWGVK 513

Query: 516 YFGKENFDRLVHVKTKVDPYDFFRHEQSIPTL 543
           YF K NFDRLV VKT VDP DFF  EQSIP +
Sbjct: 514 YF-KNNFDRLVRVKTNVDPMDFFCDEQSIPIM 529

BLAST of Csor.00g297260 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 501.5 bits (1290), Expect = 8.4e-142
Identity = 254/540 (47.04%), Postives = 361/540 (66.85%), Query Frame = 0

Query: 6   MNSSSLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYT-PFN-S 65
           MN+++   ++L LL L    S++  +F    Q+F+ CL   S     +    +T   N S
Sbjct: 6   MNNTNAFLVTLLLLSL----SYIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNAS 65

Query: 66  TYSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEG 125
           ++   L  + +NLR+  P  PKP  I  PL+ +H+QAAVLC+K   L +R RSGGHD+EG
Sbjct: 66  SFKLALETSAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEG 125

Query: 126 LSYVASL--PFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGIC 185
           LSYV+ +   F+IVDL  L  IS+D+ES +AWV +GA++GE+YY I +KS+   FPAG+C
Sbjct: 126 LSYVSEMETAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLC 185

Query: 186 PTVGVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVDRKLMGEDLFWAIRGGGGG 245
            ++G+GGH  GG YG ++RK+GL ADNV+DA++VDA+GK ++R  MGED+FWAIRGGGGG
Sbjct: 186 TSLGIGGHIIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGG 245

Query: 246 SFGIVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGGN 305
           SFG+++AWK+KLVPVP  VT+    +T E   TKL+++WQ VA KL+ + F+ +++   +
Sbjct: 246 SFGVILAWKIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTS 305

Query: 306 FTRQGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCLETSWVETALIMGTGLQ 365
            T +  +      +   +LG A+ LL ++  +FP+LGL+KKDCLETSW+++ + +  G  
Sbjct: 306 KTPKSKERTISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYI-AGFP 365

Query: 366 FGSKLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGK 425
             +  E LL   +L     K KSDY++EPIP+  +EG+WE+L  ED   P  I+ PYGG 
Sbjct: 366 STAPSEALLDGKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEED--SPLTIWNPYGGM 425

Query: 426 MGQIPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAA 485
           M +IPE ETPF HR   L+KI ++  W + G  +E +H+ W+RE+YSYM  +VSKSPR+A
Sbjct: 426 MAKIPETETPFPHRSGTLFKIQWLTLW-QDGKTSEAKHMGWMREMYSYMEQYVSKSPRSA 485

Query: 486 YLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPT 542
           Y+NYRDLD+G N K        A  WG +YF K NF+RLV +K K DP +FFRHEQSIPT
Sbjct: 486 YVNYRDLDLGMNGK-----GSDAREWGNRYF-KGNFERLVEIKAKFDPENFFRHEQSIPT 531