Csor.00g294430 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g294430
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionmechanosensitive ion channel protein 3, chloroplastic-like
LocationCsor_Chr05: 2814217 .. 2818860 (+)
RNA-Seq ExpressionCsor.00g294430
SyntenyCsor.00g294430
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTCATCATGGTTCTACCCAATTTTCGCATAAATTGGGAATTCAAAGTGTTCATGGATGCAATAAGTTACATATTGTATGTCATTCATAAACTTCTGTTTGATATGCTCAATACTTTCATTAACGGCTGGTAACTCTAATTTTCATTTCTTGATTACACGGCAGAGTGTCAAGGGAAAGGCTCGTCTACATCTTGTTACTATCGTTCCAACATCACACGGTCTGGTATGGTTGAGATACATTTGAAATTTGACAAATGTATATTAGTTGATGCTTCTATTCTCTGTGAGTTTGTGTGTGTATTAGATGTAAATATGCTCTTTTGGTGTATGTGGAACCTTGTAAATTCCTGTGCATTCTTTTGGTTGGTGAAAGGACAAAGATTTCAACATTATATGGATGTTTAAAGCTATGGATAACTTTTTTTGTTTAATTCTCAGAGGCACAAATCTGTTAGTCTTCAGCTCTTAGGCAGTGTAAGTCGCTCAATGTATCCTGTGTCTTCCAGAGCAAATGTTTTTGTGTGTCGATCTGTTCTGGAACCTAGTGGCGGAGCCGGGACTGCTGTTTTAAAATCTGCAGCTGTGGTTTTGACAAGGTGTGATTTCTTTTGATATGGAGTGCTATAGCAGAGTAAATCAAGTACATTTCCTTTTGCTAATAATCTATCCCATGGAGTTGAACTTGTTGATTTCTAGTGCTATTACTCGTCGTCATAAAATTGTAATCTTTCTCATGCTAATCTTTATAAGCTAGTAGACCTTCCTGCATTCTGCATGTGTTGATTGTATATGGTGCACATCGTGCAGACTGTTAATTTGTATCTGAAATATTCTTTAAGACCGTTTTGTGCTCATCAAATACTGACTTTTTTTTTCTTTTCTTGGGGCTTCTTCCAGATATTATGATGCGTTACGTGGCAGTCCTCTGTTACTTAAATTAATTCCAGCAGCTTGTGTCATTGCTTTTGCTGCTTGGGGTGTTGGACCTCTTATGCGTTTGGGGAGGATTCTATTTCTTCATGTATTGATCTTAATATATAATTTTATTTTATTGAGCCAGTAGAGAAAACACTTGATCAGGCTGATTAGTTATGGACATTTTGCTGCAGGAGCCAGATGGTAGTTGGAAGAAAAGTAGTACATACTTTGTTACGAGTTCTTACGTTCAACCTTTACTGCTATGGACTGGAGCTACACTGATCTGCAGGTGAGCCGTTATCTGCAGTCTGCACTATTAGGGCTAGCTAATCTTTAGCTGATGATGATACAGGTATTCACTATAGCAACTTTGTTTCAGGGCATTGGATCCAGTAGTCTTACCTTCAGTAGCCAGTCAGGCTGTCAAACAACGACTTCTGAATTTTGTTAGATCTTTGTCTACTGTTATGGCATTTGCCTATTGTATATCAAGGTGCATAGTTTCGGCGCTTCGAATTTTCATTTGAAATTTTTGAATCAGGGATGTTTACCTAGAATTGAAGTGTTGCTAAATGGATTGGGTGGCTGATATTTTAAAATTTGAATGTGTTCTTCAAAATTCTCTCTCTATGCATGCTTTATTGCATGTTTATGAGATAAAATATGCATTATTTTTTTTGTTCATCTTCTGAAATGGATCATTTACCCTTGTTAATTGTTTGTCTGATTGGACCATATCATGCATTTTGATTCTAACAGGCCTTAACCTTGATGCAGTTTGATTCAACAAGTACAAAAGTTTTCTTCTGAATCAAATGACTCGAGTGATACAAGAAATGTATGATATCCTCTCTAATTTCATCTTCTCATATTTTTCTATCATTTATACATCTAAATTTAAATTAGTTGGCACTCGCTCGCCATTAATCATTTCTAGTTAGGACAAGGGCAGATATTATGAACGGATATCCTTGAATGGATAACTTAACGGTGGAGAGCTAAGTCATAGATTAACCCTACCAACTATACTAAATAAAATCAAAATGGTTATGAAGTTAACCCGTACATGGCTACATCACTTAGATATCTTAAGTTAGTCTTTCTAAACTTGCCTTCATTTTTTGATAGATGGGCTATGATTTTGCTGGTAAAGCTGTTTACACTGCAGTCTGGATTGCTGCTCTTTCATTGTTCATGGAGTTGCTCGGTTTTTCTACCCAGAAGTGGCTGACTGCTGGGGGTCTCGGAACTGTATTGCTTACTCTTGCGGGTCGTGAGGTAAACCTGCGCTCTCCTACACGTTGTCGTTTATAAACACTACAATTATGATCTTACAATATTTTTGGGGTTGTGAAATGTGCCATTACAATTCCCTGAAGTTACTGTCTTCTTTCTATTTATTAGACGGTCTATCACCAGACAAGTTTACTCCATAATTTTGATGTATGCAGATATTTACTAACTTCCTTTCAAGCGTAATGATTCACGCAACGCGACCATTTGTGGTAAATGAATGGATTCAAACAAAGATTAATGGATATGAAGTCTCTGGAACGGTTGAGGTTTGTAACTTTTTAACACCACCACCACCTTAAGAAATATGTTGATAAGAACAGAAAGGAAAACATATCAATTTGGTTTTAGAATTTCTCTTCAAGTTTTGATTGATCGTCATTTCTGACATGGAATTTCTAAATGCAAGCATGTTGGTTGGTGGTCACCGACCATTATCCGAGGAGATGATCGTGAAGCAGTTCATATTCCGAATCACAAATTCACTGTTAGTATTGTGAGGAATCTTACCCAGAAGACTCATTGGCGTATCAAGACTCATCTTGCCATCAGCCACTTAGATGTTAATAAGATCAATGTGAGAATCAATTAGTCTTTCACACCCGTTACCTTAGTTTCTTTCATTATATGCCGTTTAATACCAAAACTTGGTTTCAATTTCCAGTATATTGTAGCTGATATGCGCAAAGTTTTGTCCAAGAATCCTCAAGTTGAACAGCAAAGGTTACACCGCAGAATTTTTTTGGATAACATCGATCCTGAAAACCAGGCTCTTATGGTATGAATTGGCTCTCATTATCTTCCTTCTGTTTCATACTTAAATTAATTGACATGTGCTAAGACTTTGATCGGTCAAATCATATGTTAATTAACAAAGAGGAGGGGTAGAACTTAAATATTATTGGTAACCTCTTCAGTACCAAGGGATTTTTTTTTTTTTTTTTTAATGATTATTTTTTCCTTTTGGAATAGAGACTAACTTCATAACGTAAAATAAACAAAAGATGTTCGTATTTCATTTTTGTTCTATATTCTTTCCCCTTCAAATTACTAACCAATTTCGTTTCGTTCCTGCAGATTATGGTTTCTTGCTTTGTGAAAACTTCTCGTTTTGAAGAATACCTGTGTGTCAAGGTATGACTGCTTTTTGGTGTTTTAGAACTTTGGTTGATTTTGTTGTTTTAGTTCTTAGTATTTTATTTTATATTTTTTCGTGTTGATGATGATCACCTCGATTCGTACTTTTGCCATGTCGTGTAACATGCGTATCAAACTATTGCAGTCTAATACATAGCTTTTAGTTGCTACATTTTCTATACTGATATAGGTCCCTACTTTCCAGTTAAGGTCTCTTTTTGTTCCTTGTCTCTGATTTTAGTTTGTTTCACAAAAATAGTATTCTTCATAACTTCTTAGTTTTCTAGATTCTTCGTTTTTAGTGTGCTTTTGAAGAATTTCTCATCAGGATTTGAATGTGGCAGTAGGTATTAATATAGTTCAACCTCATTTTCTTAATGTTGATCGTCAAGTTATTCAAGTGTCTGAACTTTCAGCCGCCAATTGATGTGCAGGAGGCAATTCTCTTGGATCTCCTCAGGGTTATCAGCCATCATAGGGCCCGGCTTGCCACTCCCATTCGCACTGTTCAGAAAATATATGGCGAGGTTGACTTGGAGAACGTACCATTTTCCGAGACCATGTATTCACGTACGGGGGCTACTAACCGTCCACTGCTTCTTATTGAGCCATCCTATAAGGTCAATGGTGACGACAAAGCCAGAGTCTCTAGAAGTCCAACTCGCTCATCAAACGAAGAAAAAGAAGCTAAACAAGAGACAGTCTCAACTACAGGGACGAAAGCCCCCGACACTGCTGGTTCAACATCAAGTATTGATATGAAAACAGATGATAGAAAGCCGGTTACTCCATCTGGTACTGCACCTAAGCCGCCTTCAACAGGGTTGTCGGTGTCAAACACTCAGACTCAGGATCTATCAACCAGTACTCCAGAAGAAAGCAGCATGGAAAAGGCAGTAACAAGCAATGAGATAAAAGGAGAAAAGAAAGATGTGATTGGACTGAATTCCAAGCTGCCCAAAAGATCACCCTCAGCTTCCAGTTCCGGCAGCGAGAAAGCTGATAGTCCTCCTCCCTCAAGCTTGCAGAACAAGCAAGACGGCGAGAAGGGCTCTGCATCATCAGTAGTGGGCAGGCCACCATTGGAGGAGAATATCGTCCTTGGAGTTGCCTTGGAAGGGTCCAAAAGAACTCTTCCAATTGATGAAGATTCAGATCCGTCTCCCTTTCACTCTCCTGCTGATTCAAAAGAAATCTCCACACAGAGGAATGGGAGCGAGTTCCCTCCCAATAGCAAGGATTTGAGAGATGGCCAGTTGCCTGCAGTTCCTGGTGCGACGAAAAATGATTGA

mRNA sequence

ATGGTTCATCATGGTTCTACCCAATTTTCGCATAAATTGGGAATTCAAAGTGTTCATGGATGCAATAAGTTACATATTAGTGTCAAGGGAAAGGCTCGTCTACATCTTGTTACTATCGTTCCAACATCACACGGTCTGAGGCACAAATCTGTTAGTCTTCAGCTCTTAGGCAGTGTAAGTCGCTCAATGTATCCTGTGTCTTCCAGAGCAAATGTTTTTGTGTGTCGATCTGTTCTGGAACCTAGTGGCGGAGCCGGGACTGCTGTTTTAAAATCTGCAGCTGTGGTTTTGACAAGATATTATGATGCGTTACGTGGCAGTCCTCTGTTACTTAAATTAATTCCAGCAGCTTGTGTCATTGCTTTTGCTGCTTGGGGTGTTGGACCTCTTATGCGTTTGGGGAGGATTCTATTTCTTCATGAGCCAGATGGTAGTTGGAAGAAAAGTAGTACATACTTTGTTACGAGTTCTTACGTTCAACCTTTACTGCTATGGACTGGAGCTACACTGATCTGCAGGGCATTGGATCCAGTAGTCTTACCTTCAGTAGCCAGTCAGGCTGTCAAACAACGACTTCTGAATTTTGTTAGATCTTTGTCTACTGTTATGGCATTTGCCTATTGTATATCAAGTTTGATTCAACAAGTACAAAAGTTTTCTTCTGAATCAAATGACTCGAGTGATACAAGAAATATGGGCTATGATTTTGCTGGTAAAGCTGTTTACACTGCAGTCTGGATTGCTGCTCTTTCATTGTTCATGGAGTTGCTCGGTTTTTCTACCCAGAAGTGGCTGACTGCTGGGGGTCTCGGAACTGTATTGCTTACTCTTGCGGGTCGTGAGATATTTACTAACTTCCTTTCAAGCGTAATGATTCACGCAACGCGACCATTTGTGGTAAATGAATGGATTCAAACAAAGATTAATGGATATGAAGTCTCTGGAACGGTTGAGCATGTTGGTTGGTGGTCACCGACCATTATCCGAGGAGATGATCGTGAAGCAGTTCATATTCCGAATCACAAATTCACTGTTAGTATTGTGAGGAATCTTACCCAGAAGACTCATTGGCGTATCAAGACTCATCTTGCCATCAGCCACTTAGATGTTAATAAGATCAATTATATTGTAGCTGATATGCGCAAAGTTTTGTCCAAGAATCCTCAAGTTGAACAGCAAAGGTTACACCGCAGAATTTTTTTGGATAACATCGATCCTGAAAACCAGGCTCTTATGATTATGGTTTCTTGCTTTGTGAAAACTTCTCGTTTTGAAGAATACCTGTGTGTCAAGGAGGCAATTCTCTTGGATCTCCTCAGGGTTATCAGCCATCATAGGGCCCGGCTTGCCACTCCCATTCGCACTGTTCAGAAAATATATGGCGAGGTTGACTTGGAGAACGTACCATTTTCCGAGACCATGTATTCACGTACGGGGGCTACTAACCGTCCACTGCTTCTTATTGAGCCATCCTATAAGGTCAATGGTGACGACAAAGCCAGAGTCTCTAGAAGTCCAACTCGCTCATCAAACGAAGAAAAAGAAGCTAAACAAGAGACAGTCTCAACTACAGGGACGAAAGCCCCCGACACTGCTGGTTCAACATCAAGTATTGATATGAAAACAGATGATAGAAAGCCGGTTACTCCATCTGGTACTGCACCTAAGCCGCCTTCAACAGGGTTGTCGGTGTCAAACACTCAGACTCAGGATCTATCAACCAGTACTCCAGAAGAAAGCAGCATGGAAAAGGCAGTAACAAGCAATGAGATAAAAGGAGAAAAGAAAGATGTGATTGGACTGAATTCCAAGCTGCCCAAAAGATCACCCTCAGCTTCCAGTTCCGGCAGCGAGAAAGCTGATAGTCCTCCTCCCTCAAGCTTGCAGAACAAGCAAGACGGCGAGAAGGGCTCTGCATCATCAGTAGTGGGCAGGCCACCATTGGAGGAGAATATCGTCCTTGGAGTTGCCTTGGAAGGGTCCAAAAGAACTCTTCCAATTGATGAAGATTCAGATCCGTCTCCCTTTCACTCTCCTGCTGATTCAAAAGAAATCTCCACACAGAGGAATGGGAGCGAGTTCCCTCCCAATAGCAAGGATTTGAGAGATGGCCAGTTGCCTGCAGTTCCTGGTGCGACGAAAAATGATTGA

Coding sequence (CDS)

ATGGTTCATCATGGTTCTACCCAATTTTCGCATAAATTGGGAATTCAAAGTGTTCATGGATGCAATAAGTTACATATTAGTGTCAAGGGAAAGGCTCGTCTACATCTTGTTACTATCGTTCCAACATCACACGGTCTGAGGCACAAATCTGTTAGTCTTCAGCTCTTAGGCAGTGTAAGTCGCTCAATGTATCCTGTGTCTTCCAGAGCAAATGTTTTTGTGTGTCGATCTGTTCTGGAACCTAGTGGCGGAGCCGGGACTGCTGTTTTAAAATCTGCAGCTGTGGTTTTGACAAGATATTATGATGCGTTACGTGGCAGTCCTCTGTTACTTAAATTAATTCCAGCAGCTTGTGTCATTGCTTTTGCTGCTTGGGGTGTTGGACCTCTTATGCGTTTGGGGAGGATTCTATTTCTTCATGAGCCAGATGGTAGTTGGAAGAAAAGTAGTACATACTTTGTTACGAGTTCTTACGTTCAACCTTTACTGCTATGGACTGGAGCTACACTGATCTGCAGGGCATTGGATCCAGTAGTCTTACCTTCAGTAGCCAGTCAGGCTGTCAAACAACGACTTCTGAATTTTGTTAGATCTTTGTCTACTGTTATGGCATTTGCCTATTGTATATCAAGTTTGATTCAACAAGTACAAAAGTTTTCTTCTGAATCAAATGACTCGAGTGATACAAGAAATATGGGCTATGATTTTGCTGGTAAAGCTGTTTACACTGCAGTCTGGATTGCTGCTCTTTCATTGTTCATGGAGTTGCTCGGTTTTTCTACCCAGAAGTGGCTGACTGCTGGGGGTCTCGGAACTGTATTGCTTACTCTTGCGGGTCGTGAGATATTTACTAACTTCCTTTCAAGCGTAATGATTCACGCAACGCGACCATTTGTGGTAAATGAATGGATTCAAACAAAGATTAATGGATATGAAGTCTCTGGAACGGTTGAGCATGTTGGTTGGTGGTCACCGACCATTATCCGAGGAGATGATCGTGAAGCAGTTCATATTCCGAATCACAAATTCACTGTTAGTATTGTGAGGAATCTTACCCAGAAGACTCATTGGCGTATCAAGACTCATCTTGCCATCAGCCACTTAGATGTTAATAAGATCAATTATATTGTAGCTGATATGCGCAAAGTTTTGTCCAAGAATCCTCAAGTTGAACAGCAAAGGTTACACCGCAGAATTTTTTTGGATAACATCGATCCTGAAAACCAGGCTCTTATGATTATGGTTTCTTGCTTTGTGAAAACTTCTCGTTTTGAAGAATACCTGTGTGTCAAGGAGGCAATTCTCTTGGATCTCCTCAGGGTTATCAGCCATCATAGGGCCCGGCTTGCCACTCCCATTCGCACTGTTCAGAAAATATATGGCGAGGTTGACTTGGAGAACGTACCATTTTCCGAGACCATGTATTCACGTACGGGGGCTACTAACCGTCCACTGCTTCTTATTGAGCCATCCTATAAGGTCAATGGTGACGACAAAGCCAGAGTCTCTAGAAGTCCAACTCGCTCATCAAACGAAGAAAAAGAAGCTAAACAAGAGACAGTCTCAACTACAGGGACGAAAGCCCCCGACACTGCTGGTTCAACATCAAGTATTGATATGAAAACAGATGATAGAAAGCCGGTTACTCCATCTGGTACTGCACCTAAGCCGCCTTCAACAGGGTTGTCGGTGTCAAACACTCAGACTCAGGATCTATCAACCAGTACTCCAGAAGAAAGCAGCATGGAAAAGGCAGTAACAAGCAATGAGATAAAAGGAGAAAAGAAAGATGTGATTGGACTGAATTCCAAGCTGCCCAAAAGATCACCCTCAGCTTCCAGTTCCGGCAGCGAGAAAGCTGATAGTCCTCCTCCCTCAAGCTTGCAGAACAAGCAAGACGGCGAGAAGGGCTCTGCATCATCAGTAGTGGGCAGGCCACCATTGGAGGAGAATATCGTCCTTGGAGTTGCCTTGGAAGGGTCCAAAAGAACTCTTCCAATTGATGAAGATTCAGATCCGTCTCCCTTTCACTCTCCTGCTGATTCAAAAGAAATCTCCACACAGAGGAATGGGAGCGAGTTCCCTCCCAATAGCAAGGATTTGAGAGATGGCCAGTTGCCTGCAGTTCCTGGTGCGACGAAAAATGATTGA

Protein sequence

MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHKSVSLQLLGSVSRSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVIAFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVLPSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKAVYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIKTHLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVKTSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSRTGATNRPLLLIEPSYKVNGDDKARVSRSPTRSSNEEKEAKQETVSTTGTKAPDTAGSTSSIDMKTDDRKPVTPSGTAPKPPSTGLSVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIGLNSKLPKRSPSASSSGSEKADSPPPSSLQNKQDGEKGSASSVVGRPPLEENIVLGVALEGSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGATKND
Homology
BLAST of Csor.00g294430 vs. ExPASy Swiss-Prot
Match: Q8L7W1 (Mechanosensitive ion channel protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MSL3 PE=1 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 5.1e-200
Identity = 390/672 (58.04%), Postives = 482/672 (71.73%), Query Frame = 0

Query: 10  SHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHKSVSLQLLGSVSRSMYPVSSR 69
           SH L +  +H  +  H S  GK R++L     +S   R    SLQLL S+S S+ PVSSR
Sbjct: 11  SHDLNVHKIHEASGFHNSAAGKNRVYLTRTGLSSCATRQDVWSLQLLESLSGSIVPVSSR 70

Query: 70  ANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVIAFAAWGVGP 129
            N FVCRS L P  G    +LKS AV+ TR YDAL G+P L+KLIPA  ++AFA WG+ P
Sbjct: 71  CNAFVCRSALSPGNGNEGPILKSTAVIFTRVYDALGGNPHLVKLIPAVGILAFATWGLRP 130

Query: 130 LMRLGR-ILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVLPSVASQAV 189
           L+RL R  LF    D + +KSST ++  SY+QPLLLW+GA L+CR LDP+VLPS A QA+
Sbjct: 131 LLRLARTTLFEKGNDANSQKSSTQYIVVSYLQPLLLWSGAILLCRTLDPIVLPSSAGQAI 190

Query: 190 KQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKAVYTAVWIA 249
           KQRLL F RS+STV+AF+ C+SSL+QQVQKF  E+N+ +DTRNMG+ FAGKAVYTA W+A
Sbjct: 191 KQRLLIFARSISTVLAFSCCLSSLLQQVQKFFMETNNPADTRNMGFSFAGKAVYTAAWVA 250

Query: 250 ALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKI 309
           A SLFMELLGFSTQKWLTAGGLGTVLLTLAGREI TNFLSS+MIHATRPFV+NEWIQTKI
Sbjct: 251 AASLFMELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIMIHATRPFVLNEWIQTKI 310

Query: 310 NGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIKTHLAISHL 369
            GYEVSGTVE VGWWSPTIIRGDDREAVHIPNH+F+V+IVRNLTQKTHWRIKTHLAISHL
Sbjct: 311 GGYEVSGTVEQVGWWSPTIIRGDDREAVHIPNHQFSVNIVRNLTQKTHWRIKTHLAISHL 370

Query: 370 DVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVKTSRFEEYL 429
           DV+KIN IVADMRKVLSKNPQ+EQQ++HRR+FL++IDPENQAL I++SCFVKTSRFEEYL
Sbjct: 371 DVSKINNIVADMRKVLSKNPQIEQQKIHRRVFLEDIDPENQALRILISCFVKTSRFEEYL 430

Query: 430 CVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSRTGATNRPLLLI 489
           CVKEA+LLDLL VI HH ARLATPIRTVQ++  E +++   FS+ ++++  A NR  +LI
Sbjct: 431 CVKEAVLLDLLTVIRHHGARLATPIRTVQRMRNEAEVDTAGFSDIVFNQ-AAMNRRYMLI 490

Query: 490 EPSYKVNGDDKARV------SRSPTRSSNEEKEAKQETVSTTGTKAPDTAGSTSSIDMKT 549
           EPSYK+N DD ++        +SP+     E+   QE  S T  +  +  GS    + K 
Sbjct: 491 EPSYKINSDDNSKSPSPSPGQKSPSPGQKSEERDLQEEPSETKAET-ENNGSVPVSNAKK 550

Query: 550 DDRKPVTPSGTAPKPPSTGLSVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIGLN 609
           +++K    S +     +TG   S+T T D   +   E   +++V  +  K EK +V    
Sbjct: 551 ENQKAALGSNS-----NTGTKGSSTSTSDQPVAQKSEEKKKESV-GDPHKAEKDEVSDDE 610

Query: 610 SKLPKRSPSASSSGSEKADSPPPSSLQNKQDGEKGSASSVVGRPPLEENIVLGVALEGSK 669
           + + +   S +  GSEK +          +DG     SS+     LEEN+VLGVAL+GSK
Sbjct: 611 ATIEQTLKSKAKQGSEKNNGE-----SKARDGGGSGTSSL-----LEENLVLGVALDGSK 664

Query: 670 RTLPIDEDSDPS 675
           RTLPIDE+   S
Sbjct: 671 RTLPIDEEHKAS 664

BLAST of Csor.00g294430 vs. ExPASy Swiss-Prot
Match: Q56X46 (Mechanosensitive ion channel protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MSL2 PE=2 SV=1)

HSP 1 Score: 672.2 bits (1733), Expect = 6.7e-192
Identity = 393/719 (54.66%), Postives = 488/719 (67.87%), Query Frame = 0

Query: 1   MVHHGSTQFSHKLGI-QSVHGCNKLHISVKGKARLHLVTIVPTSHGL---RHKSVSLQLL 60
           M  +G+ Q SH LG+ ++   CN  + ++  + RLH ++  P S G+   +H   ++ L 
Sbjct: 1   MALYGTLQLSHSLGLCRNQRFCNPENSAM--RRRLH-ISNGPLSLGVPLGQHGFSNILLS 60

Query: 61  GSVSRSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPA 120
             + R +  V  R   F C S    SG A    +K+  VVLT+ +  ++  P + KL+PA
Sbjct: 61  NYLRRPICSVPCRTTAFRCHS-FSASGKAIEPAVKAVTVVLTKSHGLMQQFPFVYKLVPA 120

Query: 121 ACVIAFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALD 180
             ++ F+ WG+ P  R GR + L++ D  WKKS TY V +SYVQPLLLW GA  ICRALD
Sbjct: 121 VALLVFSLWGLVPFARQGRNILLNKNDNGWKKSGTYHVMTSYVQPLLLWLGALFICRALD 180

Query: 181 PVVLPSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDF 240
           PVVLP+ AS+ VK RLLNFVRSLSTV+AFAYC+SSLIQQ QK  SE+++ SDTRNMG+ F
Sbjct: 181 PVVLPTEASKIVKDRLLNFVRSLSTVLAFAYCLSSLIQQTQKLFSETSNPSDTRNMGFQF 240

Query: 241 AGKAVYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATR 300
           AGKA+Y+AVW+AA+SLFMELLGFSTQKWLTAGGLGTVL+TLAGREI TNFLSSVMIHATR
Sbjct: 241 AGKALYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMIHATR 300

Query: 301 PFVVNEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTH 360
           PFV+NEWIQTKI GYEVSGTVEHVGWWSPTIIRG+DREA+HIPNHKFTV++VRNLTQKTH
Sbjct: 301 PFVLNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTVNVVRNLTQKTH 360

Query: 361 WRIKTHLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVS 420
           WRIKTHLAISHLDVNKIN IVADMRKVL+KNP VEQQRLHRR+FL+N+ PENQAL I++S
Sbjct: 361 WRIKTHLAISHLDVNKINNIVADMRKVLAKNPMVEQQRLHRRVFLENVIPENQALSILIS 420

Query: 421 CFVKTSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYS 480
           CFVKTS  EEYL VKEAILLDLLRVISHHRARLATPIRT++K+Y E D+EN PF E+MY 
Sbjct: 421 CFVKTSHHEEYLGVKEAILLDLLRVISHHRARLATPIRTIRKMYTETDVENTPFGESMYG 480

Query: 481 RTGATNRPLLLIEPSYKVNGDDKARVSRSPTRSSNEEKEAKQETVSTTGTKAPDTAGSTS 540
               + RPL+LIEP+YK+NG+DK++ S++       E+E K     +  T +PD   +  
Sbjct: 481 GV-TSRRPLMLIEPAYKINGEDKSK-SQNRAAKPTAEQENKGSNPKSKETSSPDLKANVK 540

Query: 541 SIDMKTDDRKPVTPSGTAPKPPSTGLSVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKK 600
             +    D   V P  T  KP    +S            TP+++         E  G +K
Sbjct: 541 VGESPVSDTNKV-PEETVAKPVIKAVS---------KPPTPKDT---------ETSGTEK 600

Query: 601 DVIGLNSKLPKRSPSASSSGSEKADSPPPSSLQNKQDGEKGSASSVVGRPPLEENIVLGV 660
                    PK   S  +  S K D             E  S++S   R  LEENIVLGV
Sbjct: 601 ---------PKAKRSGGTIKSTKTD-------------ETDSSTSSASRSTLEENIVLGV 660

Query: 661 ALEGSKRTLPIDEDSDPSPFHSPADSKEIS-TQRNGSEFP-PNSKDLRDGQLPAVPGAT 714
           ALEGSKRTLPI+E+    P  +  D+KE++  +R+G   P    K+ +D Q     GA+
Sbjct: 661 ALEGSKRTLPIEEEIHSPPMET--DAKELTGARRSGGNGPLVADKEQKDSQSQPNSGAS 670

BLAST of Csor.00g294430 vs. ExPASy Swiss-Prot
Match: P0AEB6 (Low conductance mechanosensitive channel YnaI OS=Escherichia coli O157:H7 OX=83334 GN=ynaI PE=3 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 6.6e-22
Identity = 77/292 (26.37%), Postives = 147/292 (50.34%), Query Frame = 0

Query: 169 TLICRALDPV---VLPSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFS-SESN 228
           T+I  A+  +   V+ +   + +   +++F+ +    +     +  LI Q +K    +  
Sbjct: 51  TIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGR 110

Query: 229 DSSDTRNMGYDFAGKAVYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFT 288
           D +  R M      + +   + +  + L+ E  G S    LT GG+G + + +AG++I +
Sbjct: 111 DITSARIM-----SRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILS 170

Query: 289 NFLSSVMIHATRPFVVNEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFT 348
           NF S +M++  RPF + +WI++     E  GTV  +GW    I   D+R  +++PN  F+
Sbjct: 171 NFFSGIMLYFDRPFSIGDWIRSPDRNIE--GTVAEIGWRITKITTFDNR-PLYVPNSLFS 230

Query: 349 VSIVRNLTQKTHWRIKTHLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNI 408
              V N  + T+ RI T + + + D  K+  IV  +R++L  +P ++Q++     F    
Sbjct: 231 SISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFA 290

Query: 409 DPENQALMIMVSCFVKTSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTV 457
           D    +L IMV CF KT+ + E+L  ++ + L ++ ++  H A  A P +T+
Sbjct: 291 D---SSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTL 331

BLAST of Csor.00g294430 vs. ExPASy Swiss-Prot
Match: P0AEB5 (Low conductance mechanosensitive channel YnaI OS=Escherichia coli (strain K12) OX=83333 GN=ynaI PE=1 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 6.6e-22
Identity = 77/292 (26.37%), Postives = 147/292 (50.34%), Query Frame = 0

Query: 169 TLICRALDPV---VLPSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFS-SESN 228
           T+I  A+  +   V+ +   + +   +++F+ +    +     +  LI Q +K    +  
Sbjct: 51  TIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGR 110

Query: 229 DSSDTRNMGYDFAGKAVYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFT 288
           D +  R M      + +   + +  + L+ E  G S    LT GG+G + + +AG++I +
Sbjct: 111 DITSARIM-----SRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILS 170

Query: 289 NFLSSVMIHATRPFVVNEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFT 348
           NF S +M++  RPF + +WI++     E  GTV  +GW    I   D+R  +++PN  F+
Sbjct: 171 NFFSGIMLYFDRPFSIGDWIRSPDRNIE--GTVAEIGWRITKITTFDNR-PLYVPNSLFS 230

Query: 349 VSIVRNLTQKTHWRIKTHLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNI 408
              V N  + T+ RI T + + + D  K+  IV  +R++L  +P ++Q++     F    
Sbjct: 231 SISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFA 290

Query: 409 DPENQALMIMVSCFVKTSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTV 457
           D    +L IMV CF KT+ + E+L  ++ + L ++ ++  H A  A P +T+
Sbjct: 291 D---SSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTL 331

BLAST of Csor.00g294430 vs. ExPASy Swiss-Prot
Match: Q58543 (Large-conductance mechanosensitive channel MscMJLR OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ1143 PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 2.9e-14
Identity = 63/250 (25.20%), Postives = 118/250 (47.20%), Query Frame = 0

Query: 179 VLPSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAG 238
           +LPS+ +   +  L  F+  L  V+ F   ++ L+++    +       D  +       
Sbjct: 85  LLPSLKTAVNEGILTAFI--LCVVVFFDRFLNELVERYLALTISKKTKKDVDDQIVVLTK 144

Query: 239 KAVYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPF 298
           K V   VW+  L L +  LG+  +  L   G+G + + LA + + +N ++ ++I   +PF
Sbjct: 145 KLVRLVVWVVGLLLILSNLGYDIKTLLAGLGIGGLAVALASQNLVSNLIAGLIILTDKPF 204

Query: 299 VVNEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWR 358
            +  WI T   G   SG VE +G  S T IR  D   + +PN K    I++N+  K  W+
Sbjct: 205 KIGNWI-TFSGG---SGIVEDIGIRS-TKIRATDNSIIVVPNSKLIDEIIQNVPSKNKWK 264

Query: 359 IKTHLAISH-LDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSC 418
           + T + +++   V KI      ++ +L ++P VE + +   ++       + +L I V  
Sbjct: 265 VSTTIGVTYNTPVEKIRKAEEIIKNILLEHPNVEDEPI--TVYFKEFG--DWSLNIQVVY 323

Query: 419 FVKTSRFEEY 428
           ++K SR+  Y
Sbjct: 325 YIKNSRYNGY 323

BLAST of Csor.00g294430 vs. NCBI nr
Match: KAG6598731.1 (Mechanosensitive ion channel protein 3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1377 bits (3564), Expect = 0.0
Identity = 716/716 (100.00%), Postives = 716/716 (100.00%), Query Frame = 0

Query: 1   MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHKSVSLQLLGSVS 60
           MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHKSVSLQLLGSVS
Sbjct: 1   MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHKSVSLQLLGSVS 60

Query: 61  RSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI 120
           RSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI
Sbjct: 61  RSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI 120

Query: 121 AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL 180
           AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL
Sbjct: 121 AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL 180

Query: 181 PSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA 240
           PSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA
Sbjct: 181 PSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA 240

Query: 241 VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300
           VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV
Sbjct: 241 VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300

Query: 301 NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK 360
           NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK
Sbjct: 301 NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK 360

Query: 361 THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVK 420
           THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVK
Sbjct: 361 THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVK 420

Query: 421 TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSRTGA 480
           TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSRTGA
Sbjct: 421 TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSRTGA 480

Query: 481 TNRPLLLIEPSYKVNGDDKARVSRSPTRSSNEEKEAKQETVSTTGTKAPDTAGSTSSIDM 540
           TNRPLLLIEPSYKVNGDDKARVSRSPTRSSNEEKEAKQETVSTTGTKAPDTAGSTSSIDM
Sbjct: 481 TNRPLLLIEPSYKVNGDDKARVSRSPTRSSNEEKEAKQETVSTTGTKAPDTAGSTSSIDM 540

Query: 541 KTDDRKPVTPSGTAPKPPSTGLSVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIG 600
           KTDDRKPVTPSGTAPKPPSTGLSVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIG
Sbjct: 541 KTDDRKPVTPSGTAPKPPSTGLSVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIG 600

Query: 601 LNSKLPKRSPSASSSGSEKADSPPPSSLQNKQDGEKGSASSVVGRPPLEENIVLGVALEG 660
           LNSKLPKRSPSASSSGSEKADSPPPSSLQNKQDGEKGSASSVVGRPPLEENIVLGVALEG
Sbjct: 601 LNSKLPKRSPSASSSGSEKADSPPPSSLQNKQDGEKGSASSVVGRPPLEENIVLGVALEG 660

Query: 661 SKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGATKND 716
           SKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGATKND
Sbjct: 661 SKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGATKND 716

BLAST of Csor.00g294430 vs. NCBI nr
Match: XP_022961790.1 (mechanosensitive ion channel protein 3, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022961791.1 mechanosensitive ion channel protein 3, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1360 bits (3519), Expect = 0.0
Identity = 709/717 (98.88%), Postives = 711/717 (99.16%), Query Frame = 0

Query: 1   MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHKSVSLQLLGSVS 60
           MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHKSVSLQLLGSVS
Sbjct: 1   MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHKSVSLQLLGSVS 60

Query: 61  RSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI 120
           RSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI
Sbjct: 61  RSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI 120

Query: 121 AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL 180
           AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL
Sbjct: 121 AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL 180

Query: 181 PSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA 240
           PSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA
Sbjct: 181 PSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA 240

Query: 241 VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300
           VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV
Sbjct: 241 VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300

Query: 301 NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK 360
           NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK
Sbjct: 301 NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK 360

Query: 361 THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVK 420
           THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVK
Sbjct: 361 THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVK 420

Query: 421 TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSRTGA 480
           TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSRTGA
Sbjct: 421 TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSRTGA 480

Query: 481 TNRPLLLIEPSYKVNGDDKARVSRSPTRSSNEEKEAKQETVSTTGTKAPDTAGSTSSIDM 540
           TNRPLLLIEPSYKVNGDDKARVSR+PTRSSNEEK+AKQETVSTTGTKAPDTAGSTSSIDM
Sbjct: 481 TNRPLLLIEPSYKVNGDDKARVSRTPTRSSNEEKDAKQETVSTTGTKAPDTAGSTSSIDM 540

Query: 541 KTDDRKPVTPSGTAPKPPSTGLSVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIG 600
           KTDDRKPVTPSGT PKPPSTG SVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIG
Sbjct: 541 KTDDRKPVTPSGTVPKPPSTGSSVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIG 600

Query: 601 LNSKLPKRSPSASSSGSEKADSPPPSSLQN-KQDGEKGSASSVVGRPPLEENIVLGVALE 660
           LNSKLPKRSPSASSSGSEKAD PPPSSLQN KQDGEK SASSVVGRPPLEENIVLGVALE
Sbjct: 601 LNSKLPKRSPSASSSGSEKADIPPPSSLQNNKQDGEKASASSVVGRPPLEENIVLGVALE 660

Query: 661 GSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGATKND 716
           GSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGAT ND
Sbjct: 661 GSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGATNND 717

BLAST of Csor.00g294430 vs. NCBI nr
Match: KAG7029673.1 (Mechanosensitive ion channel protein 3, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1358 bits (3514), Expect = 0.0
Identity = 713/741 (96.22%), Postives = 715/741 (96.49%), Query Frame = 0

Query: 1   MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHKSVSLQLLGSVS 60
           MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSH LRHKSVSLQLLGSVS
Sbjct: 1   MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHSLRHKSVSLQLLGSVS 60

Query: 61  RSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI 120
           RSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI
Sbjct: 61  RSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI 120

Query: 121 AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL 180
           AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL
Sbjct: 121 AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL 180

Query: 181 PSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA 240
           PSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA
Sbjct: 181 PSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA 240

Query: 241 VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300
           VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV
Sbjct: 241 VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300

Query: 301 NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK 360
           NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK
Sbjct: 301 NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK 360

Query: 361 THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVK 420
           THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVK
Sbjct: 361 THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVK 420

Query: 421 TSRFEEYLCVK-------------------------EAILLDLLRVISHHRARLATPIRT 480
           TSRFEEYLCVK                         EAILLDLLRVISHHRARLATPIRT
Sbjct: 421 TSRFEEYLCVKFNLIFLMLIVKLFKCLNFQPPIDVQEAILLDLLRVISHHRARLATPIRT 480

Query: 481 VQKIYGEVDLENVPFSETMYSRTGATNRPLLLIEPSYKVNGDDKARVSRSPTRSSNEEKE 540
           VQKIYGEVDLENVPFSETMYSRTGATNRPLLLIEPSYKVNGDDKARVSRSPTRSSNEEK+
Sbjct: 481 VQKIYGEVDLENVPFSETMYSRTGATNRPLLLIEPSYKVNGDDKARVSRSPTRSSNEEKD 540

Query: 541 AKQETVSTTGTKAPDTAGSTSSIDMKTDDRKPVTPSGTAPKPPSTGLSVSNTQTQDLSTS 600
           AKQETVSTTGTKAPDTAGSTSSIDMKTDDRKPVTPSGTAPKPPSTGLSVSNTQTQDLSTS
Sbjct: 541 AKQETVSTTGTKAPDTAGSTSSIDMKTDDRKPVTPSGTAPKPPSTGLSVSNTQTQDLSTS 600

Query: 601 TPEESSMEKAVTSNEIKGEKKDVIGLNSKLPKRSPSASSSGSEKADSPPPSSLQNKQDGE 660
           TPEESSMEKAVTSNEIKGEKKDVIGLNSKLPKRSPSASSSGSEKADSPPPSSLQNKQDGE
Sbjct: 601 TPEESSMEKAVTSNEIKGEKKDVIGLNSKLPKRSPSASSSGSEKADSPPPSSLQNKQDGE 660

Query: 661 KGSASSVVGRPPLEENIVLGVALEGSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFP 716
           KGSASSVVGRPPLEENIVLGVALEGSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFP
Sbjct: 661 KGSASSVVGRPPLEENIVLGVALEGSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFP 720

BLAST of Csor.00g294430 vs. NCBI nr
Match: XP_022997072.1 (mechanosensitive ion channel protein 3, chloroplastic-like isoform X1 [Cucurbita maxima] >XP_022997073.1 mechanosensitive ion channel protein 3, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1346 bits (3483), Expect = 0.0
Identity = 704/717 (98.19%), Postives = 708/717 (98.74%), Query Frame = 0

Query: 1   MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHKSVSLQLLGSVS 60
           MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRH SVSLQLLGSVS
Sbjct: 1   MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHNSVSLQLLGSVS 60

Query: 61  RSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI 120
           RSMYPVSSRANVFVCRSVLEPSGGAG AVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI
Sbjct: 61  RSMYPVSSRANVFVCRSVLEPSGGAGNAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI 120

Query: 121 AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL 180
           AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL
Sbjct: 121 AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL 180

Query: 181 PSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA 240
           PSVASQAVKQRLLNFVRSLSTV+AFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA
Sbjct: 181 PSVASQAVKQRLLNFVRSLSTVLAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA 240

Query: 241 VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300
           VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV
Sbjct: 241 VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300

Query: 301 NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK 360
           NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK
Sbjct: 301 NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK 360

Query: 361 THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVK 420
           THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNI+PENQALMIMVSCFVK
Sbjct: 361 THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNINPENQALMIMVSCFVK 420

Query: 421 TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSRTGA 480
           TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYS TGA
Sbjct: 421 TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSHTGA 480

Query: 481 TNRPLLLIEPSYKVNGDDKARVSRSPTRSSNEEKEAKQETVSTTGTKAPDTAGSTSSIDM 540
           TNRPLLLIEPSYKVNGDDKARVSRSPTRSSNEEK+AKQETVSTTGTKAPDTAGSTSSIDM
Sbjct: 481 TNRPLLLIEPSYKVNGDDKARVSRSPTRSSNEEKDAKQETVSTTGTKAPDTAGSTSSIDM 540

Query: 541 KTDDRKPVTPSGTAPKPPSTGLSVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIG 600
           KTDDRKPVT  GTAPKPPSTG SVSNTQTQDLSTSTPEESSMEKAVT+NEIKGEKKDVIG
Sbjct: 541 KTDDRKPVT--GTAPKPPSTGSSVSNTQTQDLSTSTPEESSMEKAVTNNEIKGEKKDVIG 600

Query: 601 LNSKLPKRSPSASSSGSEKADSPPPSSLQN-KQDGEKGSASSVVGRPPLEENIVLGVALE 660
           LNSKLPKRSPSASSSGSEKAD PPPSSLQN KQDGEK SASSVVGRPPLEENIVLGVALE
Sbjct: 601 LNSKLPKRSPSASSSGSEKADIPPPSSLQNNKQDGEKASASSVVGRPPLEENIVLGVALE 660

Query: 661 GSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGATKND 716
           GSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGATKND
Sbjct: 661 GSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGATKND 715

BLAST of Csor.00g294430 vs. NCBI nr
Match: XP_022961792.1 (mechanosensitive ion channel protein 3, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1333 bits (3449), Expect = 0.0
Identity = 698/717 (97.35%), Postives = 700/717 (97.63%), Query Frame = 0

Query: 1   MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHKSVSLQLLGSVS 60
           MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHKSVSLQLLGSVS
Sbjct: 1   MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHKSVSLQLLGSVS 60

Query: 61  RSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI 120
           RSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI
Sbjct: 61  RSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI 120

Query: 121 AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL 180
           AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL
Sbjct: 121 AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL 180

Query: 181 PSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA 240
           PSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA
Sbjct: 181 PSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA 240

Query: 241 VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300
           VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV
Sbjct: 241 VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300

Query: 301 NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK 360
           NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK
Sbjct: 301 NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK 360

Query: 361 THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVK 420
           THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVK
Sbjct: 361 THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVK 420

Query: 421 TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSRTGA 480
           TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSRTGA
Sbjct: 421 TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSRTGA 480

Query: 481 TNRPLLLIEPSYKVNGDDKARVSRSPTRSSNEEKEAKQETVSTTGTKAPDTAGSTSSIDM 540
           TNRPLLLIEPSYKVNGDDKARVSR+PTRSSNEEK+AKQETVSTTGTKAPDT         
Sbjct: 481 TNRPLLLIEPSYKVNGDDKARVSRTPTRSSNEEKDAKQETVSTTGTKAPDT--------- 540

Query: 541 KTDDRKPVTPSGTAPKPPSTGLSVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIG 600
             DDRKPVTPSGT PKPPSTG SVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIG
Sbjct: 541 --DDRKPVTPSGTVPKPPSTGSSVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIG 600

Query: 601 LNSKLPKRSPSASSSGSEKADSPPPSSLQN-KQDGEKGSASSVVGRPPLEENIVLGVALE 660
           LNSKLPKRSPSASSSGSEKAD PPPSSLQN KQDGEK SASSVVGRPPLEENIVLGVALE
Sbjct: 601 LNSKLPKRSPSASSSGSEKADIPPPSSLQNNKQDGEKASASSVVGRPPLEENIVLGVALE 660

Query: 661 GSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGATKND 716
           GSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGAT ND
Sbjct: 661 GSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGATNND 706

BLAST of Csor.00g294430 vs. ExPASy TrEMBL
Match: A0A6J1HBB7 (mechanosensitive ion channel protein 3, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462452 PE=4 SV=1)

HSP 1 Score: 1360 bits (3519), Expect = 0.0
Identity = 709/717 (98.88%), Postives = 711/717 (99.16%), Query Frame = 0

Query: 1   MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHKSVSLQLLGSVS 60
           MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHKSVSLQLLGSVS
Sbjct: 1   MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHKSVSLQLLGSVS 60

Query: 61  RSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI 120
           RSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI
Sbjct: 61  RSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI 120

Query: 121 AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL 180
           AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL
Sbjct: 121 AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL 180

Query: 181 PSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA 240
           PSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA
Sbjct: 181 PSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA 240

Query: 241 VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300
           VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV
Sbjct: 241 VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300

Query: 301 NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK 360
           NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK
Sbjct: 301 NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK 360

Query: 361 THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVK 420
           THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVK
Sbjct: 361 THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVK 420

Query: 421 TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSRTGA 480
           TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSRTGA
Sbjct: 421 TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSRTGA 480

Query: 481 TNRPLLLIEPSYKVNGDDKARVSRSPTRSSNEEKEAKQETVSTTGTKAPDTAGSTSSIDM 540
           TNRPLLLIEPSYKVNGDDKARVSR+PTRSSNEEK+AKQETVSTTGTKAPDTAGSTSSIDM
Sbjct: 481 TNRPLLLIEPSYKVNGDDKARVSRTPTRSSNEEKDAKQETVSTTGTKAPDTAGSTSSIDM 540

Query: 541 KTDDRKPVTPSGTAPKPPSTGLSVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIG 600
           KTDDRKPVTPSGT PKPPSTG SVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIG
Sbjct: 541 KTDDRKPVTPSGTVPKPPSTGSSVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIG 600

Query: 601 LNSKLPKRSPSASSSGSEKADSPPPSSLQN-KQDGEKGSASSVVGRPPLEENIVLGVALE 660
           LNSKLPKRSPSASSSGSEKAD PPPSSLQN KQDGEK SASSVVGRPPLEENIVLGVALE
Sbjct: 601 LNSKLPKRSPSASSSGSEKADIPPPSSLQNNKQDGEKASASSVVGRPPLEENIVLGVALE 660

Query: 661 GSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGATKND 716
           GSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGAT ND
Sbjct: 661 GSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGATNND 717

BLAST of Csor.00g294430 vs. ExPASy TrEMBL
Match: A0A6J1KCU2 (mechanosensitive ion channel protein 3, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492105 PE=4 SV=1)

HSP 1 Score: 1346 bits (3483), Expect = 0.0
Identity = 704/717 (98.19%), Postives = 708/717 (98.74%), Query Frame = 0

Query: 1   MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHKSVSLQLLGSVS 60
           MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRH SVSLQLLGSVS
Sbjct: 1   MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHNSVSLQLLGSVS 60

Query: 61  RSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI 120
           RSMYPVSSRANVFVCRSVLEPSGGAG AVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI
Sbjct: 61  RSMYPVSSRANVFVCRSVLEPSGGAGNAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI 120

Query: 121 AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL 180
           AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL
Sbjct: 121 AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL 180

Query: 181 PSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA 240
           PSVASQAVKQRLLNFVRSLSTV+AFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA
Sbjct: 181 PSVASQAVKQRLLNFVRSLSTVLAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA 240

Query: 241 VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300
           VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV
Sbjct: 241 VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300

Query: 301 NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK 360
           NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK
Sbjct: 301 NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK 360

Query: 361 THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVK 420
           THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNI+PENQALMIMVSCFVK
Sbjct: 361 THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNINPENQALMIMVSCFVK 420

Query: 421 TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSRTGA 480
           TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYS TGA
Sbjct: 421 TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSHTGA 480

Query: 481 TNRPLLLIEPSYKVNGDDKARVSRSPTRSSNEEKEAKQETVSTTGTKAPDTAGSTSSIDM 540
           TNRPLLLIEPSYKVNGDDKARVSRSPTRSSNEEK+AKQETVSTTGTKAPDTAGSTSSIDM
Sbjct: 481 TNRPLLLIEPSYKVNGDDKARVSRSPTRSSNEEKDAKQETVSTTGTKAPDTAGSTSSIDM 540

Query: 541 KTDDRKPVTPSGTAPKPPSTGLSVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIG 600
           KTDDRKPVT  GTAPKPPSTG SVSNTQTQDLSTSTPEESSMEKAVT+NEIKGEKKDVIG
Sbjct: 541 KTDDRKPVT--GTAPKPPSTGSSVSNTQTQDLSTSTPEESSMEKAVTNNEIKGEKKDVIG 600

Query: 601 LNSKLPKRSPSASSSGSEKADSPPPSSLQN-KQDGEKGSASSVVGRPPLEENIVLGVALE 660
           LNSKLPKRSPSASSSGSEKAD PPPSSLQN KQDGEK SASSVVGRPPLEENIVLGVALE
Sbjct: 601 LNSKLPKRSPSASSSGSEKADIPPPSSLQNNKQDGEKASASSVVGRPPLEENIVLGVALE 660

Query: 661 GSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGATKND 716
           GSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGATKND
Sbjct: 661 GSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGATKND 715

BLAST of Csor.00g294430 vs. ExPASy TrEMBL
Match: A0A6J1HB29 (mechanosensitive ion channel protein 3, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462452 PE=4 SV=1)

HSP 1 Score: 1333 bits (3449), Expect = 0.0
Identity = 698/717 (97.35%), Postives = 700/717 (97.63%), Query Frame = 0

Query: 1   MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHKSVSLQLLGSVS 60
           MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHKSVSLQLLGSVS
Sbjct: 1   MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHKSVSLQLLGSVS 60

Query: 61  RSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI 120
           RSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI
Sbjct: 61  RSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI 120

Query: 121 AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL 180
           AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL
Sbjct: 121 AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL 180

Query: 181 PSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA 240
           PSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA
Sbjct: 181 PSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA 240

Query: 241 VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300
           VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV
Sbjct: 241 VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300

Query: 301 NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK 360
           NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK
Sbjct: 301 NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK 360

Query: 361 THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVK 420
           THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVK
Sbjct: 361 THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVK 420

Query: 421 TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSRTGA 480
           TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSRTGA
Sbjct: 421 TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSRTGA 480

Query: 481 TNRPLLLIEPSYKVNGDDKARVSRSPTRSSNEEKEAKQETVSTTGTKAPDTAGSTSSIDM 540
           TNRPLLLIEPSYKVNGDDKARVSR+PTRSSNEEK+AKQETVSTTGTKAPDT         
Sbjct: 481 TNRPLLLIEPSYKVNGDDKARVSRTPTRSSNEEKDAKQETVSTTGTKAPDT--------- 540

Query: 541 KTDDRKPVTPSGTAPKPPSTGLSVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIG 600
             DDRKPVTPSGT PKPPSTG SVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIG
Sbjct: 541 --DDRKPVTPSGTVPKPPSTGSSVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIG 600

Query: 601 LNSKLPKRSPSASSSGSEKADSPPPSSLQN-KQDGEKGSASSVVGRPPLEENIVLGVALE 660
           LNSKLPKRSPSASSSGSEKAD PPPSSLQN KQDGEK SASSVVGRPPLEENIVLGVALE
Sbjct: 601 LNSKLPKRSPSASSSGSEKADIPPPSSLQNNKQDGEKASASSVVGRPPLEENIVLGVALE 660

Query: 661 GSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGATKND 716
           GSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGAT ND
Sbjct: 661 GSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGATNND 706

BLAST of Csor.00g294430 vs. ExPASy TrEMBL
Match: A0A6J1KAF2 (mechanosensitive ion channel protein 3, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492105 PE=4 SV=1)

HSP 1 Score: 1319 bits (3413), Expect = 0.0
Identity = 693/717 (96.65%), Postives = 697/717 (97.21%), Query Frame = 0

Query: 1   MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHKSVSLQLLGSVS 60
           MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRH SVSLQLLGSVS
Sbjct: 1   MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHNSVSLQLLGSVS 60

Query: 61  RSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI 120
           RSMYPVSSRANVFVCRSVLEPSGGAG AVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI
Sbjct: 61  RSMYPVSSRANVFVCRSVLEPSGGAGNAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI 120

Query: 121 AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL 180
           AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL
Sbjct: 121 AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL 180

Query: 181 PSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA 240
           PSVASQAVKQRLLNFVRSLSTV+AFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA
Sbjct: 181 PSVASQAVKQRLLNFVRSLSTVLAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA 240

Query: 241 VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300
           VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV
Sbjct: 241 VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300

Query: 301 NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK 360
           NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK
Sbjct: 301 NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK 360

Query: 361 THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVK 420
           THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNI+PENQALMIMVSCFVK
Sbjct: 361 THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNINPENQALMIMVSCFVK 420

Query: 421 TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSRTGA 480
           TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYS TGA
Sbjct: 421 TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSHTGA 480

Query: 481 TNRPLLLIEPSYKVNGDDKARVSRSPTRSSNEEKEAKQETVSTTGTKAPDTAGSTSSIDM 540
           TNRPLLLIEPSYKVNGDDKARVSRSPTRSSNEEK+AKQETVSTTGTKAPDT         
Sbjct: 481 TNRPLLLIEPSYKVNGDDKARVSRSPTRSSNEEKDAKQETVSTTGTKAPDT--------- 540

Query: 541 KTDDRKPVTPSGTAPKPPSTGLSVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIG 600
             DDRKPVT  GTAPKPPSTG SVSNTQTQDLSTSTPEESSMEKAVT+NEIKGEKKDVIG
Sbjct: 541 --DDRKPVT--GTAPKPPSTGSSVSNTQTQDLSTSTPEESSMEKAVTNNEIKGEKKDVIG 600

Query: 601 LNSKLPKRSPSASSSGSEKADSPPPSSLQN-KQDGEKGSASSVVGRPPLEENIVLGVALE 660
           LNSKLPKRSPSASSSGSEKAD PPPSSLQN KQDGEK SASSVVGRPPLEENIVLGVALE
Sbjct: 601 LNSKLPKRSPSASSSGSEKADIPPPSSLQNNKQDGEKASASSVVGRPPLEENIVLGVALE 660

Query: 661 GSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGATKND 716
           GSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGATKND
Sbjct: 661 GSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGATKND 704

BLAST of Csor.00g294430 vs. ExPASy TrEMBL
Match: A0A1S3BB11 (mechanosensitive ion channel protein 3, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103488126 PE=4 SV=1)

HSP 1 Score: 1187 bits (3072), Expect = 0.0
Identity = 629/723 (87.00%), Postives = 655/723 (90.59%), Query Frame = 0

Query: 1   MVHHGSTQFSHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHKSVSLQLLGSVS 60
           MVH GSTQFSHKLGIQSVHGCNKLHISVKGK RLHLVT+VPTSHGLRH   SLQLL SVS
Sbjct: 1   MVHPGSTQFSHKLGIQSVHGCNKLHISVKGKTRLHLVTVVPTSHGLRHNPGSLQLLRSVS 60

Query: 61  RSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVI 120
           R MYPVSSRANVFVCRSVLE  GGAGTAVLKSAAVVLTR  DALR SPLLLKLIPAACVI
Sbjct: 61  RPMYPVSSRANVFVCRSVLESGGGAGTAVLKSAAVVLTRSCDALRSSPLLLKLIPAACVI 120

Query: 121 AFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVL 180
           AFAAWG+GPLMRLGRILFLHEPDGSWKKSSTY+VT+SYVQPLLLWTGATLICRALDPVVL
Sbjct: 121 AFAAWGIGPLMRLGRILFLHEPDGSWKKSSTYYVTTSYVQPLLLWTGATLICRALDPVVL 180

Query: 181 PSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKA 240
           PSVASQAVKQRLLNFVRSLSTV+AFAYC+SSLIQQVQKF+ ESNDSSD RNMG+DFAGKA
Sbjct: 181 PSVASQAVKQRLLNFVRSLSTVLAFAYCLSSLIQQVQKFAYESNDSSDARNMGFDFAGKA 240

Query: 241 VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300
           VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV
Sbjct: 241 VYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300

Query: 301 NEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK 360
           NEWIQTKI+GYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK
Sbjct: 301 NEWIQTKIDGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIK 360

Query: 361 THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVK 420
           THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDN++PENQALMIMVSCFVK
Sbjct: 361 THLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNVNPENQALMIMVSCFVK 420

Query: 421 TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSRTGA 480
           TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGE DLENVPFSETMYSR+GA
Sbjct: 421 TSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEADLENVPFSETMYSRSGA 480

Query: 481 TNRPLLLIEPSYKVNGDDKARVSRSPTRSSNEEKEAKQETVSTTGTKAPDTAGSTSSIDM 540
           TNRPLLLIEPSYKVNGDDK +VS  PTRSS EEKEAKQE VST+GTKAPDT G TS++DM
Sbjct: 481 TNRPLLLIEPSYKVNGDDKTKVSSRPTRSSTEEKEAKQEAVSTSGTKAPDTTGPTSNLDM 540

Query: 541 KTDDRKPVTPSGTAPKPPSTGLSVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIG 600
           K DD+K ++PSGT PKP             +LST +PE+SS EK VTSNEIKGEKKD++G
Sbjct: 541 KADDKKSISPSGTTPKP----------SAPNLSTGSPEQSSAEKPVTSNEIKGEKKDLLG 600

Query: 601 LNSK-------LPKRSPSASSSGSEKADSPPPSSLQNKQDGEKGSASSVVGRPPLEENIV 660
           LNSK        PKRSPSASS GSEKAD P  SS QNKQDGEK SAS  V RPPLEENIV
Sbjct: 601 LNSKDNMPRGTPPKRSPSASSPGSEKADIPSTSS-QNKQDGEKTSASPSVVRPPLEENIV 660

Query: 661 LGVALEGSKRTLPIDEDSDPSPFHSPADSKEISTQRNGSEFPPNSKDLRDGQLPAVPGAT 716
           LGVALEGSKRTLPIDED          DSKEISTQRNGSEFPPNSKDL+DGQ+PAVPGAT
Sbjct: 661 LGVALEGSKRTLPIDED---------LDSKEISTQRNGSEFPPNSKDLKDGQMPAVPGAT 703

BLAST of Csor.00g294430 vs. TAIR 10
Match: AT1G58200.1 (MSCS-like 3 )

HSP 1 Score: 699.1 bits (1803), Expect = 3.6e-201
Identity = 390/672 (58.04%), Postives = 482/672 (71.73%), Query Frame = 0

Query: 10  SHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHKSVSLQLLGSVSRSMYPVSSR 69
           SH L +  +H  +  H S  GK R++L     +S   R    SLQLL S+S S+ PVSSR
Sbjct: 11  SHDLNVHKIHEASGFHNSAAGKNRVYLTRTGLSSCATRQDVWSLQLLESLSGSIVPVSSR 70

Query: 70  ANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVIAFAAWGVGP 129
            N FVCRS L P  G    +LKS AV+ TR YDAL G+P L+KLIPA  ++AFA WG+ P
Sbjct: 71  CNAFVCRSALSPGNGNEGPILKSTAVIFTRVYDALGGNPHLVKLIPAVGILAFATWGLRP 130

Query: 130 LMRLGR-ILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVLPSVASQAV 189
           L+RL R  LF    D + +KSST ++  SY+QPLLLW+GA L+CR LDP+VLPS A QA+
Sbjct: 131 LLRLARTTLFEKGNDANSQKSSTQYIVVSYLQPLLLWSGAILLCRTLDPIVLPSSAGQAI 190

Query: 190 KQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKAVYTAVWIA 249
           KQRLL F RS+STV+AF+ C+SSL+QQVQKF  E+N+ +DTRNMG+ FAGKAVYTA W+A
Sbjct: 191 KQRLLIFARSISTVLAFSCCLSSLLQQVQKFFMETNNPADTRNMGFSFAGKAVYTAAWVA 250

Query: 250 ALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKI 309
           A SLFMELLGFSTQKWLTAGGLGTVLLTLAGREI TNFLSS+MIHATRPFV+NEWIQTKI
Sbjct: 251 AASLFMELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIMIHATRPFVLNEWIQTKI 310

Query: 310 NGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIKTHLAISHL 369
            GYEVSGTVE VGWWSPTIIRGDDREAVHIPNH+F+V+IVRNLTQKTHWRIKTHLAISHL
Sbjct: 311 GGYEVSGTVEQVGWWSPTIIRGDDREAVHIPNHQFSVNIVRNLTQKTHWRIKTHLAISHL 370

Query: 370 DVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVKTSRFEEYL 429
           DV+KIN IVADMRKVLSKNPQ+EQQ++HRR+FL++IDPENQAL I++SCFVKTSRFEEYL
Sbjct: 371 DVSKINNIVADMRKVLSKNPQIEQQKIHRRVFLEDIDPENQALRILISCFVKTSRFEEYL 430

Query: 430 CVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSRTGATNRPLLLI 489
           CVKEA+LLDLL VI HH ARLATPIRTVQ++  E +++   FS+ ++++  A NR  +LI
Sbjct: 431 CVKEAVLLDLLTVIRHHGARLATPIRTVQRMRNEAEVDTAGFSDIVFNQ-AAMNRRYMLI 490

Query: 490 EPSYKVNGDDKARV------SRSPTRSSNEEKEAKQETVSTTGTKAPDTAGSTSSIDMKT 549
           EPSYK+N DD ++        +SP+     E+   QE  S T  +  +  GS    + K 
Sbjct: 491 EPSYKINSDDNSKSPSPSPGQKSPSPGQKSEERDLQEEPSETKAET-ENNGSVPVSNAKK 550

Query: 550 DDRKPVTPSGTAPKPPSTGLSVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIGLN 609
           +++K    S +     +TG   S+T T D   +   E   +++V  +  K EK +V    
Sbjct: 551 ENQKAALGSNS-----NTGTKGSSTSTSDQPVAQKSEEKKKESV-GDPHKAEKDEVSDDE 610

Query: 610 SKLPKRSPSASSSGSEKADSPPPSSLQNKQDGEKGSASSVVGRPPLEENIVLGVALEGSK 669
           + + +   S +  GSEK +          +DG     SS+     LEEN+VLGVAL+GSK
Sbjct: 611 ATIEQTLKSKAKQGSEKNNGE-----SKARDGGGSGTSSL-----LEENLVLGVALDGSK 664

Query: 670 RTLPIDEDSDPS 675
           RTLPIDE+   S
Sbjct: 671 RTLPIDEEHKAS 664

BLAST of Csor.00g294430 vs. TAIR 10
Match: AT1G58200.2 (MSCS-like 3 )

HSP 1 Score: 699.1 bits (1803), Expect = 3.6e-201
Identity = 390/672 (58.04%), Postives = 482/672 (71.73%), Query Frame = 0

Query: 10  SHKLGIQSVHGCNKLHISVKGKARLHLVTIVPTSHGLRHKSVSLQLLGSVSRSMYPVSSR 69
           SH L +  +H  +  H S  GK R++L     +S   R    SLQLL S+S S+ PVSSR
Sbjct: 11  SHDLNVHKIHEASGFHNSAAGKNRVYLTRTGLSSCATRQDVWSLQLLESLSGSIVPVSSR 70

Query: 70  ANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVIAFAAWGVGP 129
            N FVCRS L P  G    +LKS AV+ TR YDAL G+P L+KLIPA  ++AFA WG+ P
Sbjct: 71  CNAFVCRSALSPGNGNEGPILKSTAVIFTRVYDALGGNPHLVKLIPAVGILAFATWGLRP 130

Query: 130 LMRLGR-ILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVLPSVASQAV 189
           L+RL R  LF    D + +KSST ++  SY+QPLLLW+GA L+CR LDP+VLPS A QA+
Sbjct: 131 LLRLARTTLFEKGNDANSQKSSTQYIVVSYLQPLLLWSGAILLCRTLDPIVLPSSAGQAI 190

Query: 190 KQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKAVYTAVWIA 249
           KQRLL F RS+STV+AF+ C+SSL+QQVQKF  E+N+ +DTRNMG+ FAGKAVYTA W+A
Sbjct: 191 KQRLLIFARSISTVLAFSCCLSSLLQQVQKFFMETNNPADTRNMGFSFAGKAVYTAAWVA 250

Query: 250 ALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKI 309
           A SLFMELLGFSTQKWLTAGGLGTVLLTLAGREI TNFLSS+MIHATRPFV+NEWIQTKI
Sbjct: 251 AASLFMELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIMIHATRPFVLNEWIQTKI 310

Query: 310 NGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIKTHLAISHL 369
            GYEVSGTVE VGWWSPTIIRGDDREAVHIPNH+F+V+IVRNLTQKTHWRIKTHLAISHL
Sbjct: 311 GGYEVSGTVEQVGWWSPTIIRGDDREAVHIPNHQFSVNIVRNLTQKTHWRIKTHLAISHL 370

Query: 370 DVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVKTSRFEEYL 429
           DV+KIN IVADMRKVLSKNPQ+EQQ++HRR+FL++IDPENQAL I++SCFVKTSRFEEYL
Sbjct: 371 DVSKINNIVADMRKVLSKNPQIEQQKIHRRVFLEDIDPENQALRILISCFVKTSRFEEYL 430

Query: 430 CVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSRTGATNRPLLLI 489
           CVKEA+LLDLL VI HH ARLATPIRTVQ++  E +++   FS+ ++++  A NR  +LI
Sbjct: 431 CVKEAVLLDLLTVIRHHGARLATPIRTVQRMRNEAEVDTAGFSDIVFNQ-AAMNRRYMLI 490

Query: 490 EPSYKVNGDDKARV------SRSPTRSSNEEKEAKQETVSTTGTKAPDTAGSTSSIDMKT 549
           EPSYK+N DD ++        +SP+     E+   QE  S T  +  +  GS    + K 
Sbjct: 491 EPSYKINSDDNSKSPSPSPGQKSPSPGQKSEERDLQEEPSETKAET-ENNGSVPVSNAKK 550

Query: 550 DDRKPVTPSGTAPKPPSTGLSVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIGLN 609
           +++K    S +     +TG   S+T T D   +   E   +++V  +  K EK +V    
Sbjct: 551 ENQKAALGSNS-----NTGTKGSSTSTSDQPVAQKSEEKKKESV-GDPHKAEKDEVSDDE 610

Query: 610 SKLPKRSPSASSSGSEKADSPPPSSLQNKQDGEKGSASSVVGRPPLEENIVLGVALEGSK 669
           + + +   S +  GSEK +          +DG     SS+     LEEN+VLGVAL+GSK
Sbjct: 611 ATIEQTLKSKAKQGSEKNNGE-----SKARDGGGSGTSSL-----LEENLVLGVALDGSK 664

Query: 670 RTLPIDEDSDPS 675
           RTLPIDE+   S
Sbjct: 671 RTLPIDEEHKAS 664

BLAST of Csor.00g294430 vs. TAIR 10
Match: AT5G10490.1 (MSCS-like 2 )

HSP 1 Score: 672.2 bits (1733), Expect = 4.7e-193
Identity = 393/719 (54.66%), Postives = 488/719 (67.87%), Query Frame = 0

Query: 1   MVHHGSTQFSHKLGI-QSVHGCNKLHISVKGKARLHLVTIVPTSHGL---RHKSVSLQLL 60
           M  +G+ Q SH LG+ ++   CN  + ++  + RLH ++  P S G+   +H   ++ L 
Sbjct: 1   MALYGTLQLSHSLGLCRNQRFCNPENSAM--RRRLH-ISNGPLSLGVPLGQHGFSNILLS 60

Query: 61  GSVSRSMYPVSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPA 120
             + R +  V  R   F C S    SG A    +K+  VVLT+ +  ++  P + KL+PA
Sbjct: 61  NYLRRPICSVPCRTTAFRCHS-FSASGKAIEPAVKAVTVVLTKSHGLMQQFPFVYKLVPA 120

Query: 121 ACVIAFAAWGVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALD 180
             ++ F+ WG+ P  R GR + L++ D  WKKS TY V +SYVQPLLLW GA  ICRALD
Sbjct: 121 VALLVFSLWGLVPFARQGRNILLNKNDNGWKKSGTYHVMTSYVQPLLLWLGALFICRALD 180

Query: 181 PVVLPSVASQAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDF 240
           PVVLP+ AS+ VK RLLNFVRSLSTV+AFAYC+SSLIQQ QK  SE+++ SDTRNMG+ F
Sbjct: 181 PVVLPTEASKIVKDRLLNFVRSLSTVLAFAYCLSSLIQQTQKLFSETSNPSDTRNMGFQF 240

Query: 241 AGKAVYTAVWIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATR 300
           AGKA+Y+AVW+AA+SLFMELLGFSTQKWLTAGGLGTVL+TLAGREI TNFLSSVMIHATR
Sbjct: 241 AGKALYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMIHATR 300

Query: 301 PFVVNEWIQTKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTH 360
           PFV+NEWIQTKI GYEVSGTVEHVGWWSPTIIRG+DREA+HIPNHKFTV++VRNLTQKTH
Sbjct: 301 PFVLNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTVNVVRNLTQKTH 360

Query: 361 WRIKTHLAISHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVS 420
           WRIKTHLAISHLDVNKIN IVADMRKVL+KNP VEQQRLHRR+FL+N+ PENQAL I++S
Sbjct: 361 WRIKTHLAISHLDVNKINNIVADMRKVLAKNPMVEQQRLHRRVFLENVIPENQALSILIS 420

Query: 421 CFVKTSRFEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYS 480
           CFVKTS  EEYL VKEAILLDLLRVISHHRARLATPIRT++K+Y E D+EN PF E+MY 
Sbjct: 421 CFVKTSHHEEYLGVKEAILLDLLRVISHHRARLATPIRTIRKMYTETDVENTPFGESMYG 480

Query: 481 RTGATNRPLLLIEPSYKVNGDDKARVSRSPTRSSNEEKEAKQETVSTTGTKAPDTAGSTS 540
               + RPL+LIEP+YK+NG+DK++ S++       E+E K     +  T +PD   +  
Sbjct: 481 GV-TSRRPLMLIEPAYKINGEDKSK-SQNRAAKPTAEQENKGSNPKSKETSSPDLKANVK 540

Query: 541 SIDMKTDDRKPVTPSGTAPKPPSTGLSVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKK 600
             +    D   V P  T  KP    +S            TP+++         E  G +K
Sbjct: 541 VGESPVSDTNKV-PEETVAKPVIKAVS---------KPPTPKDT---------ETSGTEK 600

Query: 601 DVIGLNSKLPKRSPSASSSGSEKADSPPPSSLQNKQDGEKGSASSVVGRPPLEENIVLGV 660
                    PK   S  +  S K D             E  S++S   R  LEENIVLGV
Sbjct: 601 ---------PKAKRSGGTIKSTKTD-------------ETDSSTSSASRSTLEENIVLGV 660

Query: 661 ALEGSKRTLPIDEDSDPSPFHSPADSKEIS-TQRNGSEFP-PNSKDLRDGQLPAVPGAT 714
           ALEGSKRTLPI+E+    P  +  D+KE++  +R+G   P    K+ +D Q     GA+
Sbjct: 661 ALEGSKRTLPIEEEIHSPPMET--DAKELTGARRSGGNGPLVADKEQKDSQSQPNSGAS 670

BLAST of Csor.00g294430 vs. TAIR 10
Match: AT5G10490.3 (MSCS-like 2 )

HSP 1 Score: 666.8 bits (1719), Expect = 2.0e-191
Identity = 390/710 (54.93%), Postives = 483/710 (68.03%), Query Frame = 0

Query: 10  SHKLGI-QSVHGCNKLHISVKGKARLHLVTIVPTSHGL---RHKSVSLQLLGSVSRSMYP 69
           SH LG+ ++   CN  + ++  + RLH ++  P S G+   +H   ++ L   + R +  
Sbjct: 8   SHSLGLCRNQRFCNPENSAM--RRRLH-ISNGPLSLGVPLGQHGFSNILLSNYLRRPICS 67

Query: 70  VSSRANVFVCRSVLEPSGGAGTAVLKSAAVVLTRYYDALRGSPLLLKLIPAACVIAFAAW 129
           V  R   F C S    SG A    +K+  VVLT+ +  ++  P + KL+PA  ++ F+ W
Sbjct: 68  VPCRTTAFRCHS-FSASGKAIEPAVKAVTVVLTKSHGLMQQFPFVYKLVPAVALLVFSLW 127

Query: 130 GVGPLMRLGRILFLHEPDGSWKKSSTYFVTSSYVQPLLLWTGATLICRALDPVVLPSVAS 189
           G+ P  R GR + L++ D  WKKS TY V +SYVQPLLLW GA  ICRALDPVVLP+ AS
Sbjct: 128 GLVPFARQGRNILLNKNDNGWKKSGTYHVMTSYVQPLLLWLGALFICRALDPVVLPTEAS 187

Query: 190 QAVKQRLLNFVRSLSTVMAFAYCISSLIQQVQKFSSESNDSSDTRNMGYDFAGKAVYTAV 249
           + VK RLLNFVRSLSTV+AFAYC+SSLIQQ QK  SE+++ SDTRNMG+ FAGKA+Y+AV
Sbjct: 188 KIVKDRLLNFVRSLSTVLAFAYCLSSLIQQTQKLFSETSNPSDTRNMGFQFAGKALYSAV 247

Query: 250 WIAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQ 309
           W+AA+SLFMELLGFSTQKWLTAGGLGTVL+TLAGREI TNFLSSVMIHATRPFV+NEWIQ
Sbjct: 248 WVAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMIHATRPFVLNEWIQ 307

Query: 310 TKINGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIKTHLAI 369
           TKI GYEVSGTVEHVGWWSPTIIRG+DREA+HIPNHKFTV++VRNLTQKTHWRIKTHLAI
Sbjct: 308 TKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTVNVVRNLTQKTHWRIKTHLAI 367

Query: 370 SHLDVNKINYIVADMRKVLSKNPQVEQQRLHRRIFLDNIDPENQALMIMVSCFVKTSRFE 429
           SHLDVNKIN IVADMRKVL+KNP VEQQRLHRR+FL+N+ PENQAL I++SCFVKTS  E
Sbjct: 368 SHLDVNKINNIVADMRKVLAKNPMVEQQRLHRRVFLENVIPENQALSILISCFVKTSHHE 427

Query: 430 EYLCVKEAILLDLLRVISHHRARLATPIRTVQKIYGEVDLENVPFSETMYSRTGATNRPL 489
           EYL VKEAILLDLLRVISHHRARLATPIRT++K+Y E D+EN PF E+MY     + RPL
Sbjct: 428 EYLGVKEAILLDLLRVISHHRARLATPIRTIRKMYTETDVENTPFGESMYGGV-TSRRPL 487

Query: 490 LLIEPSYKVNGDDKARVSRSPTRSSNEEKEAKQETVSTTGTKAPDTAGSTSSIDMKTDDR 549
           +LIEP+YK+NG+DK++ S++       E+E K     +  T +PD   +    +    D 
Sbjct: 488 MLIEPAYKINGEDKSK-SQNRAAKPTAEQENKGSNPKSKETSSPDLKANVKVGESPVSDT 547

Query: 550 KPVTPSGTAPKPPSTGLSVSNTQTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIGLNSKL 609
             V P  T  KP    +S            TP+++         E  G +K         
Sbjct: 548 NKV-PEETVAKPVIKAVS---------KPPTPKDT---------ETSGTEK--------- 607

Query: 610 PKRSPSASSSGSEKADSPPPSSLQNKQDGEKGSASSVVGRPPLEENIVLGVALEGSKRTL 669
           PK   S  +  S K D             E  S++S   R  LEENIVLGVALEGSKRTL
Sbjct: 608 PKAKRSGGTIKSTKTD-------------ETDSSTSSASRSTLEENIVLGVALEGSKRTL 667

Query: 670 PIDEDSDPSPFHSPADSKEIS-TQRNGSEFP-PNSKDLRDGQLPAVPGAT 714
           PI+E+    P  +  D+KE++  +R+G   P    K+ +D Q     GA+
Sbjct: 668 PIEEEIHSPPMET--DAKELTGARRSGGNGPLVADKEQKDSQSQPNSGAS 668

BLAST of Csor.00g294430 vs. TAIR 10
Match: AT5G10490.2 (MSCS-like 2 )

HSP 1 Score: 663.7 bits (1711), Expect = 1.7e-190
Identity = 384/688 (55.81%), Postives = 471/688 (68.46%), Query Frame = 0

Query: 31  KARLHLVTIVPTSHGL---RHKSVSLQLLGSVSRSMYPVSSRANVFVCRSVLEPSGGAGT 90
           + RLH ++  P S G+   +H   ++ L   + R +  V  R   F C S    SG A  
Sbjct: 2   RRRLH-ISNGPLSLGVPLGQHGFSNILLSNYLRRPICSVPCRTTAFRCHS-FSASGKAIE 61

Query: 91  AVLKSAAVVLTRYYDALRGSPLLLKLIPAACVIAFAAWGVGPLMRLGRILFLHEPDGSWK 150
             +K+  VVLT+ +  ++  P + KL+PA  ++ F+ WG+ P  R GR + L++ D  WK
Sbjct: 62  PAVKAVTVVLTKSHGLMQQFPFVYKLVPAVALLVFSLWGLVPFARQGRNILLNKNDNGWK 121

Query: 151 KSSTYFVTSSYVQPLLLWTGATLICRALDPVVLPSVASQAVKQRLLNFVRSLSTVMAFAY 210
           KS TY V +SYVQPLLLW GA  ICRALDPVVLP+ AS+ VK RLLNFVRSLSTV+AFAY
Sbjct: 122 KSGTYHVMTSYVQPLLLWLGALFICRALDPVVLPTEASKIVKDRLLNFVRSLSTVLAFAY 181

Query: 211 CISSLIQQVQKFSSESNDSSDTRNMGYDFAGKAVYTAVWIAALSLFMELLGFSTQKWLTA 270
           C+SSLIQQ QK  SE+++ SDTRNMG+ FAGKA+Y+AVW+AA+SLFMELLGFSTQKWLTA
Sbjct: 182 CLSSLIQQTQKLFSETSNPSDTRNMGFQFAGKALYSAVWVAAVSLFMELLGFSTQKWLTA 241

Query: 271 GGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKINGYEVSGTVEHVGWWSPTI 330
           GGLGTVL+TLAGREI TNFLSSVMIHATRPFV+NEWIQTKI GYEVSGTVEHVGWWSPTI
Sbjct: 242 GGLGTVLITLAGREILTNFLSSVMIHATRPFVLNEWIQTKIEGYEVSGTVEHVGWWSPTI 301

Query: 331 IRGDDREAVHIPNHKFTVSIVRNLTQKTHWRIKTHLAISHLDVNKINYIVADMRKVLSKN 390
           IRG+DREA+HIPNHKFTV++VRNLTQKTHWRIKTHLAISHLDVNKIN IVADMRKVL+KN
Sbjct: 302 IRGEDREAIHIPNHKFTVNVVRNLTQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKN 361

Query: 391 PQVEQQRLHRRIFLDNIDPENQALMIMVSCFVKTSRFEEYLCVKEAILLDLLRVISHHRA 450
           P VEQQRLHRR+FL+N+ PENQAL I++SCFVKTS  EEYL VKEAILLDLLRVISHHRA
Sbjct: 362 PMVEQQRLHRRVFLENVIPENQALSILISCFVKTSHHEEYLGVKEAILLDLLRVISHHRA 421

Query: 451 RLATPIRTVQKIYGEVDLENVPFSETMYSRTGATNRPLLLIEPSYKVNGDDKARVSRSPT 510
           RLATPIRT++K+Y E D+EN PF E+MY     + RPL+LIEP+YK+NG+DK++ S++  
Sbjct: 422 RLATPIRTIRKMYTETDVENTPFGESMYGGV-TSRRPLMLIEPAYKINGEDKSK-SQNRA 481

Query: 511 RSSNEEKEAKQETVSTTGTKAPDTAGSTSSIDMKTDDRKPVTPSGTAPKPPSTGLSVSNT 570
                E+E K     +  T +PD   +    +    D   V P  T  KP    +S    
Sbjct: 482 AKPTAEQENKGSNPKSKETSSPDLKANVKVGESPVSDTNKV-PEETVAKPVIKAVS---- 541

Query: 571 QTQDLSTSTPEESSMEKAVTSNEIKGEKKDVIGLNSKLPKRSPSASSSGSEKADSPPPSS 630
                   TP+++         E  G +K         PK   S  +  S K D      
Sbjct: 542 -----KPPTPKDT---------ETSGTEK---------PKAKRSGGTIKSTKTD------ 601

Query: 631 LQNKQDGEKGSASSVVGRPPLEENIVLGVALEGSKRTLPIDEDSDPSPFHSPADSKEIS- 690
                  E  S++S   R  LEENIVLGVALEGSKRTLPI+E+    P  +  D+KE++ 
Sbjct: 602 -------ETDSSTSSASRSTLEENIVLGVALEGSKRTLPIEEEIHSPPMET--DAKELTG 642

Query: 691 TQRNGSEFP-PNSKDLRDGQLPAVPGAT 714
            +R+G   P    K+ +D Q     GA+
Sbjct: 662 ARRSGGNGPLVADKEQKDSQSQPNSGAS 642

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L7W15.1e-20058.04Mechanosensitive ion channel protein 3, chloroplastic OS=Arabidopsis thaliana OX... [more]
Q56X466.7e-19254.66Mechanosensitive ion channel protein 2, chloroplastic OS=Arabidopsis thaliana OX... [more]
P0AEB66.6e-2226.37Low conductance mechanosensitive channel YnaI OS=Escherichia coli O157:H7 OX=833... [more]
P0AEB56.6e-2226.37Low conductance mechanosensitive channel YnaI OS=Escherichia coli (strain K12) O... [more]
Q585432.9e-1425.20Large-conductance mechanosensitive channel MscMJLR OS=Methanocaldococcus jannasc... [more]
Match NameE-valueIdentityDescription
KAG6598731.10.0100.00Mechanosensitive ion channel protein 3, chloroplastic, partial [Cucurbita argyro... [more]
XP_022961790.10.098.88mechanosensitive ion channel protein 3, chloroplastic-like isoform X1 [Cucurbita... [more]
KAG7029673.10.096.22Mechanosensitive ion channel protein 3, chloroplastic, partial [Cucurbita argyro... [more]
XP_022997072.10.098.19mechanosensitive ion channel protein 3, chloroplastic-like isoform X1 [Cucurbita... [more]
XP_022961792.10.097.35mechanosensitive ion channel protein 3, chloroplastic-like isoform X2 [Cucurbita... [more]
Match NameE-valueIdentityDescription
A0A6J1HBB70.098.88mechanosensitive ion channel protein 3, chloroplastic-like isoform X1 OS=Cucurbi... [more]
A0A6J1KCU20.098.19mechanosensitive ion channel protein 3, chloroplastic-like isoform X1 OS=Cucurbi... [more]
A0A6J1HB290.097.35mechanosensitive ion channel protein 3, chloroplastic-like isoform X2 OS=Cucurbi... [more]
A0A6J1KAF20.096.65mechanosensitive ion channel protein 3, chloroplastic-like isoform X2 OS=Cucurbi... [more]
A0A1S3BB110.087.00mechanosensitive ion channel protein 3, chloroplastic-like OS=Cucumis melo OX=36... [more]
Match NameE-valueIdentityDescription
AT1G58200.13.6e-20158.04MSCS-like 3 [more]
AT1G58200.23.6e-20158.04MSCS-like 3 [more]
AT5G10490.14.7e-19354.66MSCS-like 2 [more]
AT5G10490.32.0e-19154.93MSCS-like 2 [more]
AT5G10490.21.7e-19055.81MSCS-like 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.287.1260coord: 192..296
e-value: 2.6E-8
score: 36.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 555..587
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 680..695
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 663..677
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 663..716
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 496..650
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 588..602
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 521..538
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 496..520
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 606..637
NoneNo IPR availablePANTHERPTHR43634:SF6MECHANOSENSITIVE ION CHANNEL PROTEIN 2, CHLOROPLASTICcoord: 1..707
IPR023408Mechanosensitive ion channel MscS domain superfamilyGENE3D2.30.30.60coord: 298..353
e-value: 7.9E-6
score: 27.9
IPR006685Mechanosensitive ion channel MscSPFAMPF00924MS_channelcoord: 239..446
e-value: 3.1E-28
score: 98.7
IPR045042Mechanosensitive ion channel protein YnaI-likePANTHERPTHR43634OW CONDUCTANCE MECHANOSENSITIVE CHANNELcoord: 1..707
IPR010920LSM domain superfamilySUPERFAMILY50182Sm-like ribonucleoproteinscoord: 282..352

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g294430.m01Csor.00g294430.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010020 chloroplast fission
biological_process GO:0006811 ion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009526 plastid envelope
cellular_component GO:0016020 membrane