Csor.00g292710 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g292710
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	purine-uracil permease NCS1-like
Location	Csor_Chr06: 2045778 .. 2046743 (-)
RNA-Seq Expression	Csor.00g292710
Synteny	Csor.00g292710

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintronterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGCTTCCGGTGTTCATGGGTGCGTTCACTTTCGTCAGCGTTGCTGTCACCTCCTCCACCGGCGTGATTTTTGGGCATGTAATTTCAAACCCAATTGATCTTCTTGGCCATATTGGTGGTGGAAAGAGGGACCGTGAAAGAGATAGGAATGGATAAATTAGATGAATGCTGCACTGCTTTATTAATGTCTACTGACTTGATAATTAGAGGGTTACACAATTCTAGTATTTATAGCTTATACACTGGACTGAATCAGAAATAGTAAATCATCGAAACCTAGAAAAAATTCAAATCTACTAAAATCTACTAAAATCTGGTAGTTTGAATATTTGAATCATATCTCAACTACTTTTAATCATATCTTCATTTTGAATACTTTGAATCATATCTCAACAATTGGGGAGTTTTGCCACTAAGATTTTGGGGATTTTTGGGATCATTTTGGCCACTGTCACCACCAATGTCGCCGCCAATGTGATGGCGCCGGCAAATGCGTTGGTCAATCTCAGCCCTTCTTATTTCACTTTCAACAGAGGGGCACTTCCCACCGCCGTGATTGGCATTTTTTTTCAGCCGTGGAGGCTTCTAAGCTCGAGTGAGAGCTTTGTTTACACTTGGCTTGTTGGGTATTCGGCGGTGATGGGGCCGATTGGCGGCGTCATGGTGGCGGATTACCATTTCATTCGGAAGAGGGAACTGATAGTTGAGGATTTGTATTCTTCGAGTCTGACAGGGGCTTATTATTACTCCGGTGGGTTTAATTTGGCGGCTGTGGCGGCGCTGGTGGTCGGAATTTTGCCGGTGATTCCTGGGTTCTTGGAGAAGGTGGAGATTTTGTCAAATGTTCCTAAAATGTTTAGGGTGATTTACGGGAATGCTTGGTTTATTAGCACCTTTTCGCCGGATTTTTCTATTGGGGTCTCTCCAATTTGTTTAAACGACCAAAAGTTTAAGCCATTTTGA

mRNA sequence

ATGGGGCTTCCGGTGTTCATGGGTGCGTTCACTTTCGTCAGCGTTGCTGTCACCTCCTCCACCGGCGTGATTTTTGGGCATATTTTGGGGATTTTTGGGATCATTTTGGCCACTGTCACCACCAATGTCGCCGCCAATGTGATGGCGCCGGCAAATGCGTTGGTCAATCTCAGCCCTTCTTATTTCACTTTCAACAGAGGGGCACTTCCCACCGCCGTGATTGGCATTTTTTTTCAGCCGTGGAGGCTTCTAAGCTCGAGTGAGAGCTTTGTTTACACTTGGCTTGTTGGGTATTCGGCGGTGATGGGGCCGATTGGCGGCGTCATGGTGGCGGATTACCATTTCATTCGGAAGAGGGAACTGATAGTTGAGGATTTGTATTCTTCGAGTCTGACAGGGGCTTATTATTACTCCGGTGGGTTTAATTTGGCGGCTGTGGCGGCGCTGGTGGTCGGAATTTTGCCGGTGATTCCTGGGTTCTTGGAGAAGGTGGAGATTTTGTCAAATGTTCCTAAAATGTTTAGGGTGATTTACGGGAATGCTTGGTTTATTAGCACCTTTTCGCCGGATTTTTCTATTGGGGTCTCTCCAATTTGTTTAAACGACCAAAAGTTTAAGCCATTTTGA

Coding sequence (CDS)

ATGGGGCTTCCGGTGTTCATGGGTGCGTTCACTTTCGTCAGCGTTGCTGTCACCTCCTCCACCGGCGTGATTTTTGGGCATATTTTGGGGATTTTTGGGATCATTTTGGCCACTGTCACCACCAATGTCGCCGCCAATGTGATGGCGCCGGCAAATGCGTTGGTCAATCTCAGCCCTTCTTATTTCACTTTCAACAGAGGGGCACTTCCCACCGCCGTGATTGGCATTTTTTTTCAGCCGTGGAGGCTTCTAAGCTCGAGTGAGAGCTTTGTTTACACTTGGCTTGTTGGGTATTCGGCGGTGATGGGGCCGATTGGCGGCGTCATGGTGGCGGATTACCATTTCATTCGGAAGAGGGAACTGATAGTTGAGGATTTGTATTCTTCGAGTCTGACAGGGGCTTATTATTACTCCGGTGGGTTTAATTTGGCGGCTGTGGCGGCGCTGGTGGTCGGAATTTTGCCGGTGATTCCTGGGTTCTTGGAGAAGGTGGAGATTTTGTCAAATGTTCCTAAAATGTTTAGGGTGATTTACGGGAATGCTTGGTTTATTAGCACCTTTTCGCCGGATTTTTCTATTGGGGTCTCTCCAATTTGTTTAAACGACCAAAAGTTTAAGCCATTTTGA

Protein sequence

MGLPVFMGAFTFVSVAVTSSTGVIFGHILGIFGIILATVTTNVAANVMAPANALVNLSPSYFTFNRGALPTAVIGIFFQPWRLLSSSESFVYTWLVGYSAVMGPIGGVMVADYHFIRKRELIVEDLYSSSLTGAYYYSGGFNLAAVAALVVGILPVIPGFLEKVEILSNVPKMFRVIYGNAWFISTFSPDFSIGVSPICLNDQKFKPF

Homology

[Close]

BLAST of Csor.00g292710 vs. ExPASy Swiss-Prot
Match: Q9LZD0 (Purine-uracil permease NCS1 OS=Arabidopsis thaliana OX=3702 GN=NCS1 PE=1 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 2.7e-61
Identity = 122/203 (60.10%), Postives = 150/203 (73.89%), Query Frame = 0


Query: 1   MGLPVFMGAFTFVSVAVTSSTGVIFGHI------------------LGIFGIILATVTTN 60
           +GLPVFMG FTFV VAVTSST +IFG +                  L I GI LAT+TTN
Sbjct: 367 VGLPVFMGLFTFVGVAVTSSTSIIFGRVISNPIELLGQIGGLATTLLAIVGISLATLTTN 426

Query: 61  VAANVMAPANALVNLSPSYFTFNRGALPTAVIGIFFQPWRLLSSSESFVYTWLVGYSAVM 120
           +AANV+APANALVNL+P +FTF RGA  TAV+GI FQPWRLL SSESFVYTWL+GYSA++
Sbjct: 427 IAANVVAPANALVNLNPKFFTFGRGAFLTAVLGIVFQPWRLLKSSESFVYTWLIGYSALL 486

Query: 121 GPIGGVMVADYHFIRKRELIVEDLYSSSLTGAYYYSGGFNLAAVAALVVGILPVIPGFLE 180
           GPIGG+++ DY+ I+K +L + DLYS S +G YY+S G+N+AAV ALV GI+PV+PGFL 
Sbjct: 487 GPIGGIILVDYYLIKKMKLNIGDLYSLSPSGEYYFSKGYNVAAVVALVAGIIPVVPGFLH 546

Query: 181 KVEILSNVPKMFRVIYGNAWFIS 186
           K+  LS +   F V+Y NA F S
Sbjct: 547 KISALSKISNGFVVVYDNALFFS 569

[Close]

BLAST of Csor.00g292710 vs. ExPASy Swiss-Prot
Match: P94575 (Probable allantoin permease OS=Bacillus subtilis (strain 168) OX=224308 GN=pucI PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 3.7e-26
Identity = 72/201 (35.82%), Postives = 108/201 (53.73%), Query Frame = 0


Query: 2   GLPVFMGAFTFVSVAVTSSTGVIFGH------------------ILGIFGIILATVTTNV 61
           GLP     F F S+ VTS + V FG                   +L +  + +AT++ NV
Sbjct: 267 GLPGTFALFAFASITVTSGSQVAFGEPIWDVVDILARFDNPYVIVLSVITLCIATISVNV 326

Query: 62  AANVMAPANALVNLSPSYFTFNRGALPTAVIGIFFQPWRLLSSSESFVYTWLVGYSAVMG 121
           AAN+++PA  + N  P Y  F RG+  TA++ +F  PW+L+ S+ S VY +L     ++G
Sbjct: 327 AANIVSPAYDIANALPKYINFKRGSFITALLALFTVPWKLMESATS-VYAFLGLIGGMLG 386

Query: 122 PIGGVMVADYHFIRKRELIVEDLYSSSLTGAYYYSGGFNLAAVAALVVGILPVIPGFLEK 181
           P+ GVM+ADY  IRKREL V+DLYS   TG Y Y  G+N  A AA ++G L         
Sbjct: 387 PVAGVMMADYFIIRKRELSVDDLYSE--TGRYVYWKGYNYRAFAATMLGAL--------- 446

Query: 182 VEILSNVPKMFRVIYGNAWFI 185
           + ++     + + +Y  +WF+
Sbjct: 447 ISLIGMYVPVLKSLYDISWFV 455

[Close]

BLAST of Csor.00g292710 vs. ExPASy Swiss-Prot
Match: Q10279 (Uracil permease OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=fur4 PE=1 SV=2)

HSP 1 Score: 91.7 bits (226), Expect = 1.1e-17
Identity = 59/184 (32.07%), Postives = 95/184 (51.63%), Query Frame = 0


Query: 18  TSSTGVIFGHILGIFGIILATVTTNVAANVMAPANALVNLSPSYFTFNRGALPTAVIGIF 77
           +++ GV  G     FG+ +A +  N+AAN ++  N L  L P+     RG    ++I + 
Sbjct: 335 SNAHGVRAGVFFIAFGLCIAQLGVNIAANSVSAGNDLSALLPTVINVRRGGYIASIIALC 394

Query: 78  FQPWRLLSSSESFVYTWLVGYSAVMGPIGGVMVADYHFIRKRELIVEDLYSSSLTGAYYY 137
             PW LLSS+ +F  T+L  YS  +    GV++ADY+F+RK  + V  LYSSS +  YY+
Sbjct: 395 MCPWNLLSSNNNFT-TYLSSYSVFLSSFAGVIIADYYFVRKGLIRVAPLYSSSSSSPYYF 454

Query: 138 SGGFNLAAVAALVVGILPVIPGFLEKVEILSNVPKMFRVIYGNAWFISTFSPDFSIGVSP 197
             G N  A A+ + G+L  I G          VPK+   +         F+ ++ +G++ 
Sbjct: 455 WKGINFRAFASYICGMLINIVGMAGSTG--QKVPKVANTM---------FNLNYFLGITV 506

Query: 198 ICLN 202
            CL+
Sbjct: 515 ACLS 506

[Close]

BLAST of Csor.00g292710 vs. ExPASy Swiss-Prot
Match: Q2UPA8 (Transporter aclS OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=aclS PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 5.0e-15
Identity = 41/111 (36.94%), Postives = 64/111 (57.66%), Query Frame = 0


Query: 54  LVNLSPSYFTFNRGALPTAVIGIFFQPWRLLSSSESFVYTWLVGYSAVMGPIGGVMVADY 113
           L  L P Y    RG     ++G+   PW LL+S+ +FV + +  +S  M P  G+ ++DY
Sbjct: 410 LSGLLPKYINIRRGCYVGLILGMALCPWELLASATTFV-SVISSFSIFMAPFCGIHISDY 469

Query: 114 HFIRKRELIVEDLYSSSLTGAYYYSGGFNLAAVAALVVGILPVIPGFLEKV 165
            FIR+R L + DLY +   G Y+Y+ GFN   V   +VG +P++PGF+  +
Sbjct: 470 WFIRQRRLKLSDLYHARPEGIYFYTMGFNWRGVLPWLVGWVPLLPGFMHSI 519

[Close]

BLAST of Csor.00g292710 vs. ExPASy Swiss-Prot
Match: O14035 (Uncharacterized permease C29B12.14c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC29B12.14c PE=3 SV=2)

HSP 1 Score: 80.1 bits (196), Expect = 3.3e-14
Identity = 60/195 (30.77%), Postives = 92/195 (47.18%), Query Frame = 0


Query: 3   LPVFMGAFTFVSVAVTSSTGVIFGHIL---------------------GIF----GIILA 62
           +P+  G  T + V V S++  I+G  +                     G+F    G+ LA
Sbjct: 302 IPMGFGITTLIGVLVGSASKSIYGENIWNPLDLLKSFLDHSNHHGVRAGVFFISTGLCLA 361

Query: 63  TVTTNVAANVMAPANALVNLSPSYFTFNRGALPTAVIGIFFQPWRLLSSSESFVYTWLVG 122
            +  N+AAN ++  N    L P +    RG    ++IGI   PW LLSSS SF  + L  
Sbjct: 362 QLGVNIAANTVSAGNDTSALCPMFINIRRGGYIASIIGICMCPWNLLSSSNSFANS-LSA 421

Query: 123 YSAVMGPIGGVMVADYHFIRKRELIVEDLYSSSLTGAYYYSGGFNLAAVAALVVGILPVI 173
           Y+  +    G+++ADY  IRK  L V+ LY+ +    Y+++ G NL A A+ + G+L  +
Sbjct: 422 YAVFLSSFAGILIADYFVIRKGYLKVDALYTINPNEPYWFTYGINLRAFASYICGLLINV 481

[Close]

BLAST of Csor.00g292710 vs. NCBI nr
Match: KAG6596516.1 (Purine-uracil permease NCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 408 bits (1049), Expect = 1.50e-143
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 0


Query: 1   MGLPVFMGAFTFVSVAVTSSTGVIFGHILGIFGIILATVTTNVAANVMAPANALVNLSPS 60
           MGLPVFMGAFTFVSVAVTSSTGVIFGHILGIFGIILATVTTNVAANVMAPANALVNLSPS
Sbjct: 1   MGLPVFMGAFTFVSVAVTSSTGVIFGHILGIFGIILATVTTNVAANVMAPANALVNLSPS 60

Query: 61  YFTFNRGALPTAVIGIFFQPWRLLSSSESFVYTWLVGYSAVMGPIGGVMVADYHFIRKRE 120
           YFTFNRGALPTAVIGIFFQPWRLLSSSESFVYTWLVGYSAVMGPIGGVMVADYHFIRKRE
Sbjct: 61  YFTFNRGALPTAVIGIFFQPWRLLSSSESFVYTWLVGYSAVMGPIGGVMVADYHFIRKRE 120

Query: 121 LIVEDLYSSSLTGAYYYSGGFNLAAVAALVVGILPVIPGFLEKVEILSNVPKMFRVIYGN 180
           LIVEDLYSSSLTGAYYYSGGFNLAAVAALVVGILPVIPGFLEKVEILSNVPKMFRVIYGN
Sbjct: 121 LIVEDLYSSSLTGAYYYSGGFNLAAVAALVVGILPVIPGFLEKVEILSNVPKMFRVIYGN 180

Query: 181 AWFISTFSPDFSIGVSPICLNDQKFKPF 208
           AWFISTFSPDFSIGVSPICLNDQKFKPF
Sbjct: 181 AWFISTFSPDFSIGVSPICLNDQKFKPF 208

[Close]

BLAST of Csor.00g292710 vs. NCBI nr
Match: XP_022959669.1 (purine-uracil permease NCS1-like [Cucurbita moschata])

HSP 1 Score: 382 bits (981), Expect = 2.82e-133
Identity = 195/202 (96.53%), Postives = 198/202 (98.02%), Query Frame = 0


Query: 7   MGAFTFVSVAVTSSTGVIFGHILGIFGIILATVTTNVAANVMAPANALVNLSPSYFTFNR 66
           MGAFTFV VAVTSSTGVIFGHIL IFGIILATVTTNVAANV+APANALVNLSPSYFTFNR
Sbjct: 1   MGAFTFVGVAVTSSTGVIFGHILAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFNR 60

Query: 67  GALPTAVIGIFFQPWRLLSSSESFVYTWLVGYSAVMGPIGGVMVADYHFIRKRELIVEDL 126
           GAL TAVIGIFFQPWRLLSSSESFVYTWLVGYSAV+GPIGGVMVADYHFIRKRELIVEDL
Sbjct: 61  GALLTAVIGIFFQPWRLLSSSESFVYTWLVGYSAVLGPIGGVMVADYHFIRKRELIVEDL 120

Query: 127 YSSSLTGAYYYSGGFNLAAVAALVVGILPVIPGFLEKVEILSNVPKMFRVIYGNAWFIST 186
           YSS+ TGAYYYSGGFNLAAVAALVVGILPVIPGFLEKVEILSNVPKMFRVIYGNAWFIST
Sbjct: 121 YSSNPTGAYYYSGGFNLAAVAALVVGILPVIPGFLEKVEILSNVPKMFRVIYGNAWFIST 180

Query: 187 FSPDFSIGVSPICLNDQKFKPF 208
           FSPDFSIGVSPICLNDQKFKPF
Sbjct: 181 FSPDFSIGVSPICLNDQKFKPF 202

[Close]

BLAST of Csor.00g292710 vs. NCBI nr
Match: XP_023005972.1 (purine-uracil permease NCS1-like [Cucurbita maxima])

HSP 1 Score: 324 bits (831), Expect = 2.79e-110
Identity = 174/211 (82.46%), Postives = 179/211 (84.83%), Query Frame = 0


Query: 7   MGAFTFVSVAVTSSTGVIFGHI------------------LGIFGIILATVTTNVAANVM 66
           MGAFTFV VAVTSSTGVIFGH+                  L IFGIILATVTTNVAANV+
Sbjct: 1   MGAFTFVGVAVTSSTGVIFGHVISNPIDLLAHIGGFTTKVLAIFGIILATVTTNVAANVV 60

Query: 67  APANALVNLSPSYFTFNRGALPTAVIGIFFQPWRLLSSSESFVYTWLVGYSAVMGPIGGV 126
           APANALVNLSPSYFTFNRGAL TAVIGI FQPW+L SSSESFVYTWLVGYSAV+GPIG V
Sbjct: 61  APANALVNLSPSYFTFNRGALLTAVIGILFQPWKLPSSSESFVYTWLVGYSAVLGPIGCV 120

Query: 127 MVADYHFIRKRELIVEDLYSSSLTGAYYYSGGFNLAAVAALVVGILPVIPGFLEKVEILS 186
           MVADYHFIRKRELIVEDLYSSS TG YYYSGGFNLAAVAALVVG+LPVIPGFLEKVEILS
Sbjct: 121 MVADYHFIRKRELIVEDLYSSSPTGVYYYSGGFNLAAVAALVVGVLPVIPGFLEKVEILS 180

Query: 187 NVPKMFRVIYGNAWFISTFSPDFSIGVSPIC 199
           NVPKMFRVIYGNAWFISTF   F   VSPIC
Sbjct: 181 NVPKMFRVIYGNAWFISTFFAGFFFWVSPIC 211

[Close]

BLAST of Csor.00g292710 vs. NCBI nr
Match: KAG7028053.1 (Purine-uracil permease NCS1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 316 bits (809), Expect = 3.25e-102
Identity = 169/222 (76.13%), Postives = 183/222 (82.43%), Query Frame = 0


Query: 1   MGLPVFMGAFTFVSVAVTSSTGVIFGHI------------------LGIFGIILATVTTN 60
           +GLPVFMGAFTFV VAVTSSTGVIFGHI                  L IFGIILATVTTN
Sbjct: 324 LGLPVFMGAFTFVGVAVTSSTGVIFGHIISNPIDLLGHIGGFATKVLAIFGIILATVTTN 383

Query: 61  VAANVMAPANALVNLSPSYFTFNRGALPTAVIGIFFQPWRLLSSSESFVYTWLVGYSAVM 120
           VAANV+APANALVNL+PSYFTF RGAL TAVIGI FQPWRLLSSSESFVYTWLVGYSA++
Sbjct: 384 VAANVVAPANALVNLNPSYFTFTRGALLTAVIGIVFQPWRLLSSSESFVYTWLVGYSALL 443

Query: 121 GPIGGVMVADYHFIRKRELIVEDLYSSSLTGAYYYSGGFNLAAVAALVVGILPVIPGFLE 180
           GPIGGVMVADY+FIR+RELIVEDLYSSS TGAYYYSGGFNL AVAALVVG+LPVIPGFLE
Sbjct: 444 GPIGGVMVADYYFIRRRELIVEDLYSSSPTGAYYYSGGFNLTAVAALVVGVLPVIPGFLE 503

Query: 181 KVEILSNVPKMFRVIYGNAWFISTFSPDFSIGVSPICLNDQK 204
           KV+I+S VP++F VIYGNAWFISTF   F      + L  +K
Sbjct: 504 KVKIVSKVPQVFTVIYGNAWFISTFIAGFCYWGLSVLLKRRK 545

[Close]

BLAST of Csor.00g292710 vs. NCBI nr
Match: KAG6596517.1 (Purine-uracil permease NCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 316 bits (809), Expect = 4.40e-102
Identity = 169/222 (76.13%), Postives = 183/222 (82.43%), Query Frame = 0


Query: 1   MGLPVFMGAFTFVSVAVTSSTGVIFGHI------------------LGIFGIILATVTTN 60
           +GLPVFMGAFTFV VAVTSSTGVIFGHI                  L IFGIILATVTTN
Sbjct: 335 LGLPVFMGAFTFVGVAVTSSTGVIFGHIISNPIDLLGHIGGFATKVLAIFGIILATVTTN 394

Query: 61  VAANVMAPANALVNLSPSYFTFNRGALPTAVIGIFFQPWRLLSSSESFVYTWLVGYSAVM 120
           VAANV+APANALVNL+PSYFTF RGAL TAVIGI FQPWRLLSSSESFVYTWLVGYSA++
Sbjct: 395 VAANVVAPANALVNLNPSYFTFTRGALLTAVIGIVFQPWRLLSSSESFVYTWLVGYSALL 454

Query: 121 GPIGGVMVADYHFIRKRELIVEDLYSSSLTGAYYYSGGFNLAAVAALVVGILPVIPGFLE 180
           GPIGGVMVADY+FIR+RELIVEDLYSSS TGAYYYSGGFNL AVAALVVG+LPVIPGFLE
Sbjct: 455 GPIGGVMVADYYFIRRRELIVEDLYSSSPTGAYYYSGGFNLTAVAALVVGVLPVIPGFLE 514

Query: 181 KVEILSNVPKMFRVIYGNAWFISTFSPDFSIGVSPICLNDQK 204
           KV+I+S VP++F VIYGNAWFISTF   F      + L  +K
Sbjct: 515 KVKIVSKVPQVFTVIYGNAWFISTFIAGFCYWGLSVLLKRRK 556

[Close]

BLAST of Csor.00g292710 vs. ExPASy TrEMBL
Match: A0A6J1H8S0 (purine-uracil permease NCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111460652 PE=3 SV=1)

HSP 1 Score: 382 bits (981), Expect = 1.36e-133
Identity = 195/202 (96.53%), Postives = 198/202 (98.02%), Query Frame = 0


Query: 7   MGAFTFVSVAVTSSTGVIFGHILGIFGIILATVTTNVAANVMAPANALVNLSPSYFTFNR 66
           MGAFTFV VAVTSSTGVIFGHIL IFGIILATVTTNVAANV+APANALVNLSPSYFTFNR
Sbjct: 1   MGAFTFVGVAVTSSTGVIFGHILAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFNR 60

Query: 67  GALPTAVIGIFFQPWRLLSSSESFVYTWLVGYSAVMGPIGGVMVADYHFIRKRELIVEDL 126
           GAL TAVIGIFFQPWRLLSSSESFVYTWLVGYSAV+GPIGGVMVADYHFIRKRELIVEDL
Sbjct: 61  GALLTAVIGIFFQPWRLLSSSESFVYTWLVGYSAVLGPIGGVMVADYHFIRKRELIVEDL 120

Query: 127 YSSSLTGAYYYSGGFNLAAVAALVVGILPVIPGFLEKVEILSNVPKMFRVIYGNAWFIST 186
           YSS+ TGAYYYSGGFNLAAVAALVVGILPVIPGFLEKVEILSNVPKMFRVIYGNAWFIST
Sbjct: 121 YSSNPTGAYYYSGGFNLAAVAALVVGILPVIPGFLEKVEILSNVPKMFRVIYGNAWFIST 180

Query: 187 FSPDFSIGVSPICLNDQKFKPF 208
           FSPDFSIGVSPICLNDQKFKPF
Sbjct: 181 FSPDFSIGVSPICLNDQKFKPF 202

[Close]

BLAST of Csor.00g292710 vs. ExPASy TrEMBL
Match: A0A6J1KUM8 (purine-uracil permease NCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111498832 PE=3 SV=1)

HSP 1 Score: 324 bits (831), Expect = 1.35e-110
Identity = 174/211 (82.46%), Postives = 179/211 (84.83%), Query Frame = 0


Query: 7   MGAFTFVSVAVTSSTGVIFGHI------------------LGIFGIILATVTTNVAANVM 66
           MGAFTFV VAVTSSTGVIFGH+                  L IFGIILATVTTNVAANV+
Sbjct: 1   MGAFTFVGVAVTSSTGVIFGHVISNPIDLLAHIGGFTTKVLAIFGIILATVTTNVAANVV 60

Query: 67  APANALVNLSPSYFTFNRGALPTAVIGIFFQPWRLLSSSESFVYTWLVGYSAVMGPIGGV 126
           APANALVNLSPSYFTFNRGAL TAVIGI FQPW+L SSSESFVYTWLVGYSAV+GPIG V
Sbjct: 61  APANALVNLSPSYFTFNRGALLTAVIGILFQPWKLPSSSESFVYTWLVGYSAVLGPIGCV 120

Query: 127 MVADYHFIRKRELIVEDLYSSSLTGAYYYSGGFNLAAVAALVVGILPVIPGFLEKVEILS 186
           MVADYHFIRKRELIVEDLYSSS TG YYYSGGFNLAAVAALVVG+LPVIPGFLEKVEILS
Sbjct: 121 MVADYHFIRKRELIVEDLYSSSPTGVYYYSGGFNLAAVAALVVGVLPVIPGFLEKVEILS 180

Query: 187 NVPKMFRVIYGNAWFISTFSPDFSIGVSPIC 199
           NVPKMFRVIYGNAWFISTF   F   VSPIC
Sbjct: 181 NVPKMFRVIYGNAWFISTFFAGFFFWVSPIC 211

[Close]

BLAST of Csor.00g292710 vs. ExPASy TrEMBL
Match: A0A6J1KWR9 (purine-uracil permease NCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111498292 PE=3 SV=1)

HSP 1 Score: 316 bits (809), Expect = 2.13e-102
Identity = 169/222 (76.13%), Postives = 183/222 (82.43%), Query Frame = 0


Query: 1   MGLPVFMGAFTFVSVAVTSSTGVIFGHI------------------LGIFGIILATVTTN 60
           +GLPVFMGAFTFV VAVTSSTGVIFGHI                  L IFGIILATVTTN
Sbjct: 335 LGLPVFMGAFTFVGVAVTSSTGVIFGHIISNPIDLLGHIGGFATKVLAIFGIILATVTTN 394

Query: 61  VAANVMAPANALVNLSPSYFTFNRGALPTAVIGIFFQPWRLLSSSESFVYTWLVGYSAVM 120
           VAANV+APANALVNLSPSYFTF RGAL TAVIGI FQPWRLLSSSESFVYTWLVGYSA++
Sbjct: 395 VAANVVAPANALVNLSPSYFTFTRGALLTAVIGIVFQPWRLLSSSESFVYTWLVGYSALL 454

Query: 121 GPIGGVMVADYHFIRKRELIVEDLYSSSLTGAYYYSGGFNLAAVAALVVGILPVIPGFLE 180
           GPIGGVMVADY+F+RKRELIVEDLYSSS TGAYYYSGGFNL AVAALVVG+LPVIPGFLE
Sbjct: 455 GPIGGVMVADYYFLRKRELIVEDLYSSSPTGAYYYSGGFNLTAVAALVVGVLPVIPGFLE 514

Query: 181 KVEILSNVPKMFRVIYGNAWFISTFSPDFSIGVSPICLNDQK 204
           KV+I+S VP++F VIYGNAWFIS+F   F      + L  +K
Sbjct: 515 KVKIVSKVPEVFTVIYGNAWFISSFIAGFCYWGLSVLLKRRK 556

[Close]

BLAST of Csor.00g292710 vs. ExPASy TrEMBL
Match: A0A6J1GDF7 (purine-uracil permease NCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111453146 PE=3 SV=1)

HSP 1 Score: 313 bits (802), Expect = 2.40e-101
Identity = 169/223 (75.78%), Postives = 187/223 (83.86%), Query Frame = 0


Query: 1   MGLPVFMGAFTFVSVAVTSSTGVIFGHI------------------LGIFGIILATVTTN 60
           +GLPVFMGAFTFV VAVTSSTGVIFGHI                  L IFGIILATVTTN
Sbjct: 335 LGLPVFMGAFTFVGVAVTSSTGVIFGHIISNPIDLLGHIGGFATKVLAIFGIILATVTTN 394

Query: 61  VAANVMAPANALVNLSPSYFTFNRGALPTAVIGIFFQPWRLLSSSESFVYTWLVGYSAVM 120
           VAANV+APANALVNL+PSYFTF RGAL TAVIGI FQPWRLLSSSESFVYTWLVGYSA++
Sbjct: 395 VAANVVAPANALVNLNPSYFTFTRGALLTAVIGIVFQPWRLLSSSESFVYTWLVGYSALL 454

Query: 121 GPIGGVMVADYHFIRKRELIVEDLYSSSLTGAYYYSGGFNLAAVAALVVGILPVIPGFLE 180
           GPIGGVMVADY+FIR+RELIVEDLYSSS TGAYY+SGGFNL AVAALVVG+LPVIPGFLE
Sbjct: 455 GPIGGVMVADYYFIRRRELIVEDLYSSSPTGAYYFSGGFNLTAVAALVVGVLPVIPGFLE 514

Query: 181 KVEILSNVPKMFRVIYGNAWFISTFSPDFS-IGVSPICLNDQK 204
           KV+++S VP++F VIYGNAWFISTF   F  +G+S + L  +K
Sbjct: 515 KVKLVSKVPQLFTVIYGNAWFISTFIAGFCYLGLS-VLLKRRK 556

[Close]

BLAST of Csor.00g292710 vs. ExPASy TrEMBL
Match: A0A5D3DPI8 (Purine-uracil permease NCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G003170 PE=3 SV=1)

HSP 1 Score: 303 bits (775), Expect = 2.03e-97
Identity = 162/222 (72.97%), Postives = 177/222 (79.73%), Query Frame = 0


Query: 1   MGLPVFMGAFTFVSVAVTSSTGVIFGHI------------------LGIFGIILATVTTN 60
           +GLP+FMGAFTFV VAVTSSTGVIFGHI                  L IFGIILATVTTN
Sbjct: 324 LGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPIDLLGHIGGFVTKVLAIFGIILATVTTN 383

Query: 61  VAANVMAPANALVNLSPSYFTFNRGALPTAVIGIFFQPWRLLSSSESFVYTWLVGYSAVM 120
           VAANV+APANALVNLSPSYFTF RGAL TAVIGI FQPWRLLSSSESFVYTWLVGYSA++
Sbjct: 384 VAANVVAPANALVNLSPSYFTFTRGALLTAVIGILFQPWRLLSSSESFVYTWLVGYSALL 443

Query: 121 GPIGGVMVADYHFIRKRELIVEDLYSSSLTGAYYYSGGFNLAAVAALVVGILPVIPGFLE 180
           GPIGGVMV DY+ IR +EL VEDLYS+S  GAYYYSGGFNL AV ALVVG+LPVIPGFLE
Sbjct: 444 GPIGGVMVTDYYLIRGKELSVEDLYSTSPRGAYYYSGGFNLVAVVALVVGVLPVIPGFLE 503

Query: 181 KVEILSNVPKMFRVIYGNAWFISTFSPDFSIGVSPICLNDQK 204
           KVEI+S VPK+F VIYG+AWFIS+F   F      + L  +K
Sbjct: 504 KVEIVSKVPKVFSVIYGSAWFISSFLAGFCYWGLSVLLKRRK 545

[Close]

BLAST of Csor.00g292710 vs. TAIR 10
Match: AT5G03555.1 (permease, cytosine/purines, uracil, thiamine, allantoin family protein )

HSP 1 Score: 236.5 bits (602), Expect = 1.9e-62
Identity = 122/203 (60.10%), Postives = 150/203 (73.89%), Query Frame = 0


Query: 1   MGLPVFMGAFTFVSVAVTSSTGVIFGHI------------------LGIFGIILATVTTN 60
           +GLPVFMG FTFV VAVTSST +IFG +                  L I GI LAT+TTN
Sbjct: 367 VGLPVFMGLFTFVGVAVTSSTSIIFGRVISNPIELLGQIGGLATTLLAIVGISLATLTTN 426

Query: 61  VAANVMAPANALVNLSPSYFTFNRGALPTAVIGIFFQPWRLLSSSESFVYTWLVGYSAVM 120
           +AANV+APANALVNL+P +FTF RGA  TAV+GI FQPWRLL SSESFVYTWL+GYSA++
Sbjct: 427 IAANVVAPANALVNLNPKFFTFGRGAFLTAVLGIVFQPWRLLKSSESFVYTWLIGYSALL 486

Query: 121 GPIGGVMVADYHFIRKRELIVEDLYSSSLTGAYYYSGGFNLAAVAALVVGILPVIPGFLE 180
           GPIGG+++ DY+ I+K +L + DLYS S +G YY+S G+N+AAV ALV GI+PV+PGFL 
Sbjct: 487 GPIGGIILVDYYLIKKMKLNIGDLYSLSPSGEYYFSKGYNVAAVVALVAGIIPVVPGFLH 546

Query: 181 KVEILSNVPKMFRVIYGNAWFIS 186
           K+  LS +   F V+Y NA F S
Sbjct: 547 KISALSKISNGFVVVYDNALFFS 569

The following BLAST results are available for this feature:

BLAST of Csor.00g292710 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LZD0	2.7e-61	60.10	Purine-uracil permease NCS1 OS=Arabidopsis thaliana OX=3702 GN=NCS1 PE=1 SV=1	[more]
P94575	3.7e-26	35.82	Probable allantoin permease OS=Bacillus subtilis (strain 168) OX=224308 GN=pucI ...	[more]
Q10279	1.1e-17	32.07	Uracil permease OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812...	[more]
Q2UPA8	5.0e-15	36.94	Transporter aclS OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN...	[more]
O14035	3.3e-14	30.77	Uncharacterized permease C29B12.14c OS=Schizosaccharomyces pombe (strain 972 / A...	[more]

BLAST of Csor.00g292710 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6596516.1	1.50e-143	100.00	Purine-uracil permease NCS1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022959669.1	2.82e-133	96.53	purine-uracil permease NCS1-like [Cucurbita moschata]	[more]
XP_023005972.1	2.79e-110	82.46	purine-uracil permease NCS1-like [Cucurbita maxima]	[more]
KAG7028053.1	3.25e-102	76.13	Purine-uracil permease NCS1, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]
KAG6596517.1	4.40e-102	76.13	Purine-uracil permease NCS1, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Csor.00g292710 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H8S0	1.36e-133	96.53	purine-uracil permease NCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111460652 P...	[more]
A0A6J1KUM8	1.35e-110	82.46	purine-uracil permease NCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111498832 PE=...	[more]
A0A6J1KWR9	2.13e-102	76.13	purine-uracil permease NCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111498292 PE=...	[more]
A0A6J1GDF7	2.40e-101	75.78	purine-uracil permease NCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111453146 P...	[more]
A0A5D3DPI8	2.03e-97	72.97	Purine-uracil permease NCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]

BLAST of Csor.00g292710 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G03555.1	1.9e-62	60.10	permease, cytosine/purines, uracil, thiamine, allantoin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001248	Purine-cytosine permease	PFAM	PF02133	Transp_cyt_pur	coord: 21..179 e-value: 2.6E-32 score: 112.2
None	No IPR available	GENE3D	1.10.4160.10	Hydantoin permease	coord: 2..193 e-value: 1.3E-38 score: 134.5
None	No IPR available	PANTHER	PTHR30618:SF0	PURINE-URACIL PERMEASE NCS1	coord: 2..187
None	No IPR available	PANTHER	PTHR30618	NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER	coord: 2..187

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g292710.m01	Csor.00g292710.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015851	nucleobase transport
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0015205	nucleobase transmembrane transporter activity
molecular_function	GO:0022857	transmembrane transporter activity