Csor.00g291890 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g291890
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionPolcalcin Jun o
LocationCsor_Chr04: 15916822 .. 15917391 (+)
RNA-Seq ExpressionCsor.00g291890
SyntenyCsor.00g291890
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSsinglestart_codonpolypeptidestop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGATTCAAATCTCTCTTCTCCCGGAAGAAGAAATCCGATTCCTCCAGCGATTCACCGCTCGGATCCACCACGGTTCCGATTCTCGGATCTCGAACTCCGTCTTTCAATTCTCGAGCGCAAATCGCGGAATTGGAGCAAGTATTCAAGAAATTCGATGTCAATGGCGACGGCAAGATCTGCTCCTCTGAACTCGGTTCCATTATGGGGTGCCTAGGCCAGCCGGCCACGGAGGAGGAGCTTCAGAACATGATCAAGGAAGTCGATGCCGATGGCGACGGCTACATTGACCTCCATGAGTTCATCGAACTCAACACCAAGGGAGTCGATTCCGACGAGGTGCTTAAGAATCTGAAGGACGCATTCTCCGTCTATGACCTTGACGGCAACGGATCCATCACGGCGGAGGAATTGCACGAGGTCTTGAAGAGTTTGGGCGACGACTGCTCGCTGGCGGATTGCCGGAACATGATCAGCGGCGTCGACAGAAACGGCGACGGTATGATTAGTTTCGATGAATTCAAAGCCATGATGATGAGCGGTTCTCGATCTCACGGCCTCAGTGATTAG

mRNA sequence

ATGGGATTCAAATCTCTCTTCTCCCGGAAGAAGAAATCCGATTCCTCCAGCGATTCACCGCTCGGATCCACCACGGTTCCGATTCTCGGATCTCGAACTCCGTCTTTCAATTCTCGAGCGCAAATCGCGGAATTGGAGCAAGTATTCAAGAAATTCGATGTCAATGGCGACGGCAAGATCTGCTCCTCTGAACTCGGTTCCATTATGGGGTGCCTAGGCCAGCCGGCCACGGAGGAGGAGCTTCAGAACATGATCAAGGAAGTCGATGCCGATGGCGACGGCTACATTGACCTCCATGAGTTCATCGAACTCAACACCAAGGGAGTCGATTCCGACGAGGTGCTTAAGAATCTGAAGGACGCATTCTCCGTCTATGACCTTGACGGCAACGGATCCATCACGGCGGAGGAATTGCACGAGGTCTTGAAGAGTTTGGGCGACGACTGCTCGCTGGCGGATTGCCGGAACATGATCAGCGGCGTCGACAGAAACGGCGACGGTATGATTAGTTTCGATGAATTCAAAGCCATGATGATGAGCGGTTCTCGATCTCACGGCCTCAGTGATTAG

Coding sequence (CDS)

ATGGGATTCAAATCTCTCTTCTCCCGGAAGAAGAAATCCGATTCCTCCAGCGATTCACCGCTCGGATCCACCACGGTTCCGATTCTCGGATCTCGAACTCCGTCTTTCAATTCTCGAGCGCAAATCGCGGAATTGGAGCAAGTATTCAAGAAATTCGATGTCAATGGCGACGGCAAGATCTGCTCCTCTGAACTCGGTTCCATTATGGGGTGCCTAGGCCAGCCGGCCACGGAGGAGGAGCTTCAGAACATGATCAAGGAAGTCGATGCCGATGGCGACGGCTACATTGACCTCCATGAGTTCATCGAACTCAACACCAAGGGAGTCGATTCCGACGAGGTGCTTAAGAATCTGAAGGACGCATTCTCCGTCTATGACCTTGACGGCAACGGATCCATCACGGCGGAGGAATTGCACGAGGTCTTGAAGAGTTTGGGCGACGACTGCTCGCTGGCGGATTGCCGGAACATGATCAGCGGCGTCGACAGAAACGGCGACGGTATGATTAGTTTCGATGAATTCAAAGCCATGATGATGAGCGGTTCTCGATCTCACGGCCTCAGTGATTAG

Protein sequence

MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKICSSELGSIMGCLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKDAFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMSGSRSHGLSD
Homology
BLAST of Csor.00g291890 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 1.5e-53
Identity = 106/182 (58.24%), Postives = 137/182 (75.27%), Query Frame = 0

Query: 6   LFSRKKKSDSSSDSPLGSTTVPILGSRTP----SFNSRAQIAELEQVFKKFDVNGDGKIC 65
           +F++ + S+  S S +G      +G+ +P    + + + +I ELE VFKKFDVNGDGKI 
Sbjct: 1   MFNKNQGSNGGSSSNVG------IGADSPYLQKARSGKTEIRELEAVFKKFDVNGDGKIS 60

Query: 66  SSELGSIMGCLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKDA 125
           S ELG+IM  LG    EEEL+  I E+D  GDGYI+  EF+ELNTKG+D ++VL+NLKDA
Sbjct: 61  SKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDA 120

Query: 126 FSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMSG 184
           FSVYD+DGNGSI+AEELHEVL+SLGD+CS+A+CR MI GVD++GDG I F+EFK MM  G
Sbjct: 121 FSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDGDGTIDFEEFKIMMTMG 176

BLAST of Csor.00g291890 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 4.7e-36
Identity = 86/178 (48.31%), Postives = 113/178 (63.48%), Query Frame = 0

Query: 5   SLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKICSSE 64
           +LF R+    S S SP      P  G  +P+        E+E+VF+KFD NGDG+I  SE
Sbjct: 8   ALFRRR----SGSKSPPLPQADPASGGGSPAPTPE---EEMERVFRKFDANGDGRISRSE 67

Query: 65  LGSIMGCLGQPATEEELQNMIKEVDADGDGYIDLHEFIELN-TKGVDSDEVLKNLKDAFS 124
           LG++   LG  AT++EL  M+ E DADGDG+I L EF  LN T   D+  V ++L+ AF 
Sbjct: 68  LGALFESLGHAATDDELARMMAEADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFR 127

Query: 125 VYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMSG 182
           V+D DGNG+I+A EL  VL  LG+  ++  CR MI GVD+NGDG+ISF+EFK MM  G
Sbjct: 128 VFDADGNGTISAAELARVLHGLGEKATVQQCRRMIEGVDQNGDGLISFEEFKVMMAGG 178

BLAST of Csor.00g291890 vs. ExPASy Swiss-Prot
Match: O64943 (Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2)

HSP 1 Score: 150.6 bits (379), Expect = 1.8e-35
Identity = 73/139 (52.52%), Postives = 100/139 (71.94%), Query Frame = 0

Query: 42  IAELEQVFKKFDVNGDGKICSSELGSIMGCLGQPATEEELQNMIKEVDADGDGYIDLHEF 101
           + ELE+VFKKFD NGDGKI  SEL  I+  LG    E E++ M++E DADGDGY+ L EF
Sbjct: 24  VHELEEVFKKFDANGDGKISGSELADILRSLGSDVGEAEVKAMMEEADADGDGYVSLQEF 83

Query: 102 IELNTKGVDSDEVLKNLKDAFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGV 161
           ++LN KG      +K+LK+AF V+D D NGSI+A EL   L+S+G+ C++ + +N+I  V
Sbjct: 84  VDLNNKGAS----VKDLKNAFKVFDRDCNGSISAAELCHTLESVGEPCTIEESKNIIHNV 143

Query: 162 DRNGDGMISFDEFKAMMMS 181
           D+NGDG+IS +EF+ MM S
Sbjct: 144 DKNGDGLISVEEFQTMMTS 158

BLAST of Csor.00g291890 vs. ExPASy Swiss-Prot
Match: A0T2M3 (Polcalcin Cup a 4 OS=Hesperocyparis arizonica OX=49011 PE=1 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.2e-34
Identity = 70/139 (50.36%), Postives = 99/139 (71.22%), Query Frame = 0

Query: 42  IAELEQVFKKFDVNGDGKICSSELGSIMGCLGQPATEEELQNMIKEVDADGDGYIDLHEF 101
           + ELE+VFKKFD NGDGKI  SEL  I+  +G    E E++ M++E D DGDGY+ L EF
Sbjct: 24  VHELEEVFKKFDANGDGKISGSELADILRSMGSEVDEAEVKAMMEEADTDGDGYVSLQEF 83

Query: 102 IELNTKGVDSDEVLKNLKDAFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGV 161
           ++LN KG      +K+LK+AF V+D D NG+I+  EL E LKS+G+ C++ + +N+I  V
Sbjct: 84  VDLNIKGA----TVKDLKNAFKVFDRDCNGTISPAELCETLKSVGEPCTIEESKNIIHNV 143

Query: 162 DRNGDGMISFDEFKAMMMS 181
           D+NGDG+I+ +EF+ MM S
Sbjct: 144 DKNGDGLINVEEFQTMMTS 158

BLAST of Csor.00g291890 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 2.2e-33
Identity = 69/151 (45.70%), Postives = 104/151 (68.87%), Query Frame = 0

Query: 31  SRTPSFNSRAQIAELEQVFKKFDVNGDGKICSSELGSIMGCLGQPATEEELQNMIKEVDA 90
           S+  S N    + ++++VF++FD N DGKI   EL  ++G L   A++EE + M+KE D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 91  DGDGYIDLHEFIEL--NTKGVDSDEVLKNLKDAFSVYDLDGNGSITAEELHEVLKSLGDD 150
           DG+G+IDL EF+ L   +    ++  +++LK+AF +YDLD NG I+A ELH V+K+LG+ 
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 151 CSLADCRNMISGVDRNGDGMISFDEFKAMMM 180
           CS+ DC+ MI+ VD +GDG + F+EFK MMM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMMM 152

BLAST of Csor.00g291890 vs. NCBI nr
Match: KAG6601861.1 (putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032560.1 putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 369 bits (947), Expect = 1.25e-128
Identity = 189/189 (100.00%), Postives = 189/189 (100.00%), Query Frame = 0

Query: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGCLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120
           CSSELGSIMGCLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD
Sbjct: 61  CSSELGSIMGCLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180
           AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180

Query: 181 GSRSHGLSD 189
           GSRSHGLSD
Sbjct: 181 GSRSHGLSD 189

BLAST of Csor.00g291890 vs. NCBI nr
Match: XP_022963580.1 (probable calcium-binding protein CML25 [Cucurbita moschata])

HSP 1 Score: 365 bits (937), Expect = 4.20e-127
Identity = 188/189 (99.47%), Postives = 188/189 (99.47%), Query Frame = 0

Query: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGCLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120
           CSSELGSIMG LGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180
           AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180

Query: 181 GSRSHGLSD 189
           GSRSHGLSD
Sbjct: 181 GSRSHGLSD 189

BLAST of Csor.00g291890 vs. NCBI nr
Match: XP_022990613.1 (probable calcium-binding protein CML25 [Cucurbita maxima])

HSP 1 Score: 364 bits (935), Expect = 8.47e-127
Identity = 187/189 (98.94%), Postives = 188/189 (99.47%), Query Frame = 0

Query: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGCLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120
           CSSELGSIMG LGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180
           AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180

Query: 181 GSRSHGLSD 189
           GSRSHG+SD
Sbjct: 181 GSRSHGISD 189

BLAST of Csor.00g291890 vs. NCBI nr
Match: XP_023513887.1 (probable calcium-binding protein CML25 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 363 bits (933), Expect = 1.71e-126
Identity = 187/189 (98.94%), Postives = 187/189 (98.94%), Query Frame = 0

Query: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGCLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120
           CSSELGSIMG LGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180
           AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180

Query: 181 GSRSHGLSD 189
           GSRSHGL D
Sbjct: 181 GSRSHGLGD 189

BLAST of Csor.00g291890 vs. NCBI nr
Match: XP_022146331.1 (probable calcium-binding protein CML25 [Momordica charantia])

HSP 1 Score: 330 bits (846), Expect = 3.13e-113
Identity = 168/186 (90.32%), Postives = 177/186 (95.16%), Query Frame = 0

Query: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSD+SSDSP GST VP+LGSRTPSFN R QIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGCLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120
            SSELGSIMG LGQPATEEE+QNMIKEVDADGDG+IDL EFIELNTKGVDS+EVL+NLKD
Sbjct: 61  SSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKD 120

Query: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180
           AFSVYD+DGNGSITAEELHEVLKSLGD+CSLADCR MI+GVDRNGDGMISFDEFK MMMS
Sbjct: 121 AFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMS 180

Query: 181 GSRSHG 186
           GSRSHG
Sbjct: 181 GSRSHG 186

BLAST of Csor.00g291890 vs. ExPASy TrEMBL
Match: A0A6J1HID9 (probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC111463873 PE=4 SV=1)

HSP 1 Score: 365 bits (937), Expect = 2.03e-127
Identity = 188/189 (99.47%), Postives = 188/189 (99.47%), Query Frame = 0

Query: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGCLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120
           CSSELGSIMG LGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180
           AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180

Query: 181 GSRSHGLSD 189
           GSRSHGLSD
Sbjct: 181 GSRSHGLSD 189

BLAST of Csor.00g291890 vs. ExPASy TrEMBL
Match: A0A6J1JTS9 (probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC111487441 PE=4 SV=1)

HSP 1 Score: 364 bits (935), Expect = 4.10e-127
Identity = 187/189 (98.94%), Postives = 188/189 (99.47%), Query Frame = 0

Query: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGCLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120
           CSSELGSIMG LGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180
           AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180

Query: 181 GSRSHGLSD 189
           GSRSHG+SD
Sbjct: 181 GSRSHGISD 189

BLAST of Csor.00g291890 vs. ExPASy TrEMBL
Match: A0A6J1CZ20 (probable calcium-binding protein CML25 OS=Momordica charantia OX=3673 GN=LOC111015568 PE=4 SV=1)

HSP 1 Score: 330 bits (846), Expect = 1.52e-113
Identity = 168/186 (90.32%), Postives = 177/186 (95.16%), Query Frame = 0

Query: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSD+SSDSP GST VP+LGSRTPSFN R QIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGCLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120
            SSELGSIMG LGQPATEEE+QNMIKEVDADGDG+IDL EFIELNTKGVDS+EVL+NLKD
Sbjct: 61  SSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKD 120

Query: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180
           AFSVYD+DGNGSITAEELHEVLKSLGD+CSLADCR MI+GVDRNGDGMISFDEFK MMMS
Sbjct: 121 AFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMS 180

Query: 181 GSRSHG 186
           GSRSHG
Sbjct: 181 GSRSHG 186

BLAST of Csor.00g291890 vs. ExPASy TrEMBL
Match: A0A0A0KST2 (Polcalcin Jun o OS=Cucumis sativus OX=3659 GN=Csa_5G561760 PE=4 SV=1)

HSP 1 Score: 317 bits (813), Expect = 1.27e-108
Identity = 166/188 (88.30%), Postives = 173/188 (92.02%), Query Frame = 0

Query: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKS SS+DSPLGSTT+PILGSRT       QIAELEQVF KFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSHSSTDSPLGSTTLPILGSRT-------QIAELEQVFNKFDVNGDGKI 60

Query: 61  CSSELGSIMGCLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120
           CSSELGSIMG LGQPATEEELQNMIKEVDADGDGYIDL EFIELNTKGVDSDEVL+NLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKD 120

Query: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180
           AFSVYD+DGNGSITAEELHEVLKSLGDDCSLADCR MI+GVD+NGDGMISFDEFK MMMS
Sbjct: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMS 180

Query: 181 GSRSHGLS 188
           GSRS G +
Sbjct: 181 GSRSQGFN 181

BLAST of Csor.00g291890 vs. ExPASy TrEMBL
Match: A0A5A7TS48 (Putative calcium-binding protein CML25 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3042G00010 PE=4 SV=1)

HSP 1 Score: 313 bits (802), Expect = 6.03e-107
Identity = 163/188 (86.70%), Postives = 171/188 (90.96%), Query Frame = 0

Query: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKS  S+DSPLGSTT+PI GSRT       QIAELEQVF KFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSHPSTDSPLGSTTLPIFGSRT-------QIAELEQVFNKFDVNGDGKI 60

Query: 61  CSSELGSIMGCLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120
           CSSELGSIMG LGQPATEEEL NMIKEVDADGDGYIDL EFIELNTKGVDS+EVL+NLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKD 120

Query: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180
           AFSVYD+DGNGSITAEELHEVLKSLGDDCSLADCR MI+GVD+NGDGMISFDEFK MMMS
Sbjct: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMS 180

Query: 181 GSRSHGLS 188
           GSRSHG +
Sbjct: 181 GSRSHGFN 181

BLAST of Csor.00g291890 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 210.7 bits (535), Expect = 1.0e-54
Identity = 106/182 (58.24%), Postives = 137/182 (75.27%), Query Frame = 0

Query: 6   LFSRKKKSDSSSDSPLGSTTVPILGSRTP----SFNSRAQIAELEQVFKKFDVNGDGKIC 65
           +F++ + S+  S S +G      +G+ +P    + + + +I ELE VFKKFDVNGDGKI 
Sbjct: 1   MFNKNQGSNGGSSSNVG------IGADSPYLQKARSGKTEIRELEAVFKKFDVNGDGKIS 60

Query: 66  SSELGSIMGCLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKDA 125
           S ELG+IM  LG    EEEL+  I E+D  GDGYI+  EF+ELNTKG+D ++VL+NLKDA
Sbjct: 61  SKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDA 120

Query: 126 FSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMSG 184
           FSVYD+DGNGSI+AEELHEVL+SLGD+CS+A+CR MI GVD++GDG I F+EFK MM  G
Sbjct: 121 FSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDGDGTIDFEEFKIMMTMG 176

BLAST of Csor.00g291890 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 143.7 bits (361), Expect = 1.5e-34
Identity = 69/151 (45.70%), Postives = 104/151 (68.87%), Query Frame = 0

Query: 31  SRTPSFNSRAQIAELEQVFKKFDVNGDGKICSSELGSIMGCLGQPATEEELQNMIKEVDA 90
           S+  S N    + ++++VF++FD N DGKI   EL  ++G L   A++EE + M+KE D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 91  DGDGYIDLHEFIEL--NTKGVDSDEVLKNLKDAFSVYDLDGNGSITAEELHEVLKSLGDD 150
           DG+G+IDL EF+ L   +    ++  +++LK+AF +YDLD NG I+A ELH V+K+LG+ 
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 151 CSLADCRNMISGVDRNGDGMISFDEFKAMMM 180
           CS+ DC+ MI+ VD +GDG + F+EFK MMM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMMM 152

BLAST of Csor.00g291890 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 143.3 bits (360), Expect = 2.0e-34
Identity = 69/142 (48.59%), Postives = 101/142 (71.13%), Query Frame = 0

Query: 44  ELEQVFKKFDVNGDGKICSSELGSIMGCLGQPATEEELQNMIKEVDADGDGYIDLHEFIE 103
           ++++VF++FD NGDGKI   EL  ++  L   A+ EE   M+K+ D DG+G+IDL EF+ 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 104 LNTKGV----DSDEVLKNLKDAFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMIS 163
           L   G+    ++   + +LK+AF +YDLDGNG I+A+ELH V+K+LG+ CS+ DC+ MIS
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 164 GVDRNGDGMISFDEFKAMMMSG 182
            VD +GDG ++FDEFK MM +G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNG 158

BLAST of Csor.00g291890 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 134.8 bits (338), Expect = 7.2e-32
Identity = 72/156 (46.15%), Postives = 94/156 (60.26%), Query Frame = 0

Query: 27  PILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKICSSELGSIMGCLGQPATEEELQNMIK 86
           P     TP+    A   EL++VF +FD NGDGKI   ELG +   +G   TE EL  +++
Sbjct: 6   PETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLE 65

Query: 87  EVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKDAFSVYDLDGNGSITAEELHEVLKSLG 146
           EVD D DGYI+L EF  L      + E+    +DAF +YD D NG I+A ELH+VL  LG
Sbjct: 66  EVDTDRDGYINLDEFSTLCRSSSSAAEI----RDAFDLYDQDKNGLISASELHQVLNRLG 125

Query: 147 DDCSLADCRNMISGVDRNGDGMISFDEFKAMMMSGS 183
             CS+ DC  MI  VD +GDG ++F+EF+ MM S S
Sbjct: 126 MSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS 157

BLAST of Csor.00g291890 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 134.8 bits (338), Expect = 7.2e-32
Identity = 72/156 (46.15%), Postives = 94/156 (60.26%), Query Frame = 0

Query: 27  PILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKICSSELGSIMGCLGQPATEEELQNMIK 86
           P     TP+    A   EL++VF +FD NGDGKI   ELG +   +G   TE EL  +++
Sbjct: 6   PETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLE 65

Query: 87  EVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKDAFSVYDLDGNGSITAEELHEVLKSLG 146
           EVD D DGYI+L EF  L      + E+    +DAF +YD D NG I+A ELH+VL  LG
Sbjct: 66  EVDTDRDGYINLDEFSTLCRSSSSAAEI----RDAFDLYDQDKNGLISASELHQVLNRLG 125

Query: 147 DDCSLADCRNMISGVDRNGDGMISFDEFKAMMMSGS 183
             CS+ DC  MI  VD +GDG ++F+EF+ MM S S
Sbjct: 126 MSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS 157

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FYK21.5e-5358.24Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ... [more]
Q8RZB54.7e-3648.31Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
O649431.8e-3552.52Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2[more]
A0T2M31.2e-3450.36Polcalcin Cup a 4 OS=Hesperocyparis arizonica OX=49011 PE=1 SV=1[more]
Q9C8Y12.2e-3345.70Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ... [more]
Match NameE-valueIdentityDescription
KAG6601861.11.25e-128100.00putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. s... [more]
XP_022963580.14.20e-12799.47probable calcium-binding protein CML25 [Cucurbita moschata][more]
XP_022990613.18.47e-12798.94probable calcium-binding protein CML25 [Cucurbita maxima][more]
XP_023513887.11.71e-12698.94probable calcium-binding protein CML25 [Cucurbita pepo subsp. pepo][more]
XP_022146331.13.13e-11390.32probable calcium-binding protein CML25 [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A6J1HID92.03e-12799.47probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
A0A6J1JTS94.10e-12798.94probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC1114874... [more]
A0A6J1CZ201.52e-11390.32probable calcium-binding protein CML25 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A0A0KST21.27e-10888.30Polcalcin Jun o OS=Cucumis sativus OX=3659 GN=Csa_5G561760 PE=4 SV=1[more]
A0A5A7TS486.03e-10786.70Putative calcium-binding protein CML25 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
Match NameE-valueIdentityDescription
AT1G24620.11.0e-5458.24EF hand calcium-binding protein family [more]
AT1G66400.11.5e-3445.70calmodulin like 23 [more]
AT5G37770.12.0e-3448.59EF hand calcium-binding protein family [more]
AT1G18210.17.2e-3246.15Calcium-binding EF-hand family protein [more]
AT1G18210.27.2e-3246.15Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 80..108
e-value: 6.7E-5
score: 32.3
coord: 44..72
e-value: 2.9E-5
score: 33.5
coord: 117..145
e-value: 2.0E-5
score: 34.0
coord: 153..181
e-value: 1.9E-5
score: 34.1
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 44..103
e-value: 4.6E-13
score: 49.4
coord: 117..178
e-value: 4.1E-12
score: 46.4
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 40..75
score: 15.071314
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 149..184
score: 12.78389
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 76..111
score: 13.537066
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 113..148
score: 15.182896
IPR002048EF-hand domainCDDcd00051EFhcoord: 118..179
e-value: 1.21987E-20
score: 79.1289
IPR002048EF-hand domainCDDcd00051EFhcoord: 44..104
e-value: 2.38945E-19
score: 75.6621
NoneNo IPR availableGENE3D1.10.238.10coord: 107..185
e-value: 4.2E-25
score: 89.9
coord: 31..106
e-value: 2.5E-24
score: 87.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..33
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 17..33
NoneNo IPR availablePANTHERPTHR23050:SF382CALCIUM-BINDING PROTEIN CML25-RELATEDcoord: 27..184
NoneNo IPR availablePANTHERPTHR23050CALCIUM BINDING PROTEINcoord: 27..184
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 89..101
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 126..138
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 53..65
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 162..174
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 32..180

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g291890.m01Csor.00g291890.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050790 regulation of catalytic activity
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0030234 enzyme regulator activity