Csor.00g291550 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g291550
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionCytochrome b561 domain-containing protein
LocationCsor_Chr04: 16201053 .. 16201961 (-)
RNA-Seq ExpressionCsor.00g291550
SyntenyCsor.00g291550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACACTGTGAACAGGCCAGGTTTCAGGCATACTGCCTCTGCCATTGCAGCCCTTGCACATATTTTTGGTCTCACTGCCTGCATTCTTACGCTTGTTTGGCTCCTGCATTATCGAGGCGGCCTCGCCTACGATTCTCGGAGTGCCGACCTCGTTTTCAATGTAAGGCTCACATTTAATTTGTAGTTTTAAAGGAACTGATATTAAGAGATTTACTTTTGGCAGTGCCACCCGTTCTTCATGCTCCTTGGTTTCGTGATCGTGTCGGGGGAAGGTATAGCTCGATCGAGTTTGTTTAATTTATATCTTGAGATGATTCATAACTATAATTGAATTGGATTTGAGCAGCAATGATGGCTTACAAGACAGTGAGGGCAGACAAAATGACAAGGAAGATAGTTCATATGGCGTTGCATTTTGTAGCCATTTGTATGGGAATTGTAGGCATAAATGCTTCTTTCAAGTTCCATGATATGATCAAATTGGAGCATATGTTTAGTTTGCATTCGTGGATTGGCATTGTCACTTTCTGCCTCTATGGCTTACAGGTACATAATATAATATAATATGCTGTTTGTCGTTAAAGTTTCAATTATGTTCTTAAACTTTTATACACGAATGGCATGGCAGTGGCTGTTGGGTCTGTGCACGTTCTTGTTTCCAGGAGCAGGCAGTGAGACCAAAGCCAGGCTGCTTCCATGGCACATAAGTGGGGGAAGGGCTCTGCTGCTGATGATTATCTGCACAGCCTTGACTGGTTTGTCTCAGAAGGCTACTTCTTCGACACTCACTGGCAACCATGAGTCTCGTTTGCTTAAGTTCACTGGTTTCTTCGTCCTCCTCTTCGGCATTTCCATCGATATCGCCATCGGTTTTGATCGGTTTCGCCACGTCGATCCCACGACTTAA

mRNA sequence

ATGGACACTGTGAACAGGCCAGGTTTCAGGCATACTGCCTCTGCCATTGCAGCCCTTGCACATATTTTTGGTCTCACTGCCTGCATTCTTACGCTTGTTTGGCTCCTGCATTATCGAGGCGGCCTCGCCTACGATTCTCGGAGTGCCGACCTCGTTTTCAATTGCCACCCGTTCTTCATGCTCCTTGGTTTCGTGATCGTGTCGGGGGAAGCAATGATGGCTTACAAGACAGTGAGGGCAGACAAAATGACAAGGAAGATAGTTCATATGGCGTTGCATTTTGTAGCCATTTGTATGGGAATTGTAGGCATAAATGCTTCTTTCAAGTTCCATGATATGATCAAATTGGAGCATATGTTTAGTTTGCATTCGTGGATTGGCATTGTCACTTTCTGCCTCTATGGCTTACAGTGGCTGTTGGGTCTGTGCACGTTCTTGTTTCCAGGAGCAGGCAGTGAGACCAAAGCCAGGCTGCTTCCATGGCACATAAGTGGGGGAAGGGCTCTGCTGCTGATGATTATCTGCACAGCCTTGACTGGTTTGTCTCAGAAGGCTACTTCTTCGACACTCACTGGCAACCATGAGTCTCGTTTGCTTAAGTTCACTGGTTTCTTCGTCCTCCTCTTCGGCATTTCCATCGATATCGCCATCGGTTTTGATCGGTTTCGCCACGTCGATCCCACGACTTAA

Coding sequence (CDS)

ATGGACACTGTGAACAGGCCAGGTTTCAGGCATACTGCCTCTGCCATTGCAGCCCTTGCACATATTTTTGGTCTCACTGCCTGCATTCTTACGCTTGTTTGGCTCCTGCATTATCGAGGCGGCCTCGCCTACGATTCTCGGAGTGCCGACCTCGTTTTCAATTGCCACCCGTTCTTCATGCTCCTTGGTTTCGTGATCGTGTCGGGGGAAGCAATGATGGCTTACAAGACAGTGAGGGCAGACAAAATGACAAGGAAGATAGTTCATATGGCGTTGCATTTTGTAGCCATTTGTATGGGAATTGTAGGCATAAATGCTTCTTTCAAGTTCCATGATATGATCAAATTGGAGCATATGTTTAGTTTGCATTCGTGGATTGGCATTGTCACTTTCTGCCTCTATGGCTTACAGTGGCTGTTGGGTCTGTGCACGTTCTTGTTTCCAGGAGCAGGCAGTGAGACCAAAGCCAGGCTGCTTCCATGGCACATAAGTGGGGGAAGGGCTCTGCTGCTGATGATTATCTGCACAGCCTTGACTGGTTTGTCTCAGAAGGCTACTTCTTCGACACTCACTGGCAACCATGAGTCTCGTTTGCTTAAGTTCACTGGTTTCTTCGTCCTCCTCTTCGGCATTTCCATCGATATCGCCATCGGTTTTGATCGGTTTCGCCACGTCGATCCCACGACTTAA

Protein sequence

MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFMLLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMFSLHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTGLSQKATSSTLTGNHESRLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT
Homology
BLAST of Csor.00g291550 vs. ExPASy Swiss-Prot
Match: Q67ZF6 (Probable transmembrane ascorbate ferrireductase 3 OS=Arabidopsis thaliana OX=3702 GN=CYB561C PE=2 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 2.2e-56
Identity = 106/218 (48.62%), Postives = 143/218 (65.60%), Query Frame = 0

Query: 5   NRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFMLLGF 64
           +R   +  +S    +AH FG+ A +L L+WLLHYR G+ Y S +   V N HPF M  GF
Sbjct: 6   DRTTLKRHSSLSTLVAHFFGILAVVLMLIWLLHYREGIEYGSDNPLKVLNVHPFLMYCGF 65

Query: 65  VIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMFSLHS 124
           + + G+AMM YKT  A    +K+VH  LH + + +GIVGI A+F+FHD + L+ M SLHS
Sbjct: 66  LFLVGQAMMTYKTAYASHQVQKMVHGGLHLIGLVLGIVGICAAFRFHDKVNLKDMVSLHS 125

Query: 125 WIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTGLSQK 184
           WIG+ TF L G+QWL G  TFL P + S T+ R++PWH+ GGRALL M I  ALTGL Q+
Sbjct: 126 WIGLTTFILLGVQWLFGAFTFLAPQSSSGTRTRMMPWHVLGGRALLYMGIVAALTGLMQR 185

Query: 185 ATSSTLTGNHESRLLKFTGFFVLLFGISIDIAIGFDRF 223
           AT    + N ESRL+ F G  +LLFG+S+D ++   R+
Sbjct: 186 ATMLGQSTNAESRLINFLGLAILLFGVSVDFSVALGRY 223

BLAST of Csor.00g291550 vs. ExPASy Swiss-Prot
Match: A3A9H6 (Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0642300 PE=3 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 9.1e-42
Identity = 83/209 (39.71%), Postives = 128/209 (61.24%), Query Frame = 0

Query: 13  ASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFMLLGFVIVSGEAM 72
           A+    +AH   + A  + LVW +H+RGGLA+++ + +L+FN HP  ML+G++I+  EA+
Sbjct: 7   AAPFTYVAHALAVAAATMVLVWCIHFRGGLAFEATNKNLIFNVHPVLMLIGYIILGSEAI 66

Query: 73  MAYKTVRADKM-TRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMFSLHSWIGIVTF 132
           M YK +   K  T K++H+ LH +A+  G VGI  +FKFH+   + +++SLHSW+GI T 
Sbjct: 67  MVYKVLPTWKHDTTKLIHLILHAIALVFGAVGIYCAFKFHNESGIANLYSLHSWLGIGTI 126

Query: 133 CLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTGLSQKATSSTLT 192
           CLYG+QW+ G   F FP A    +  +LPWHI  G  + ++ + TA  G  +K T    +
Sbjct: 127 CLYGIQWIFGFVAFFFPRASPSVRKGVLPWHILFGLFVYILALATAELGFLEKLTFLQSS 186

Query: 193 G----NHESRLLKFTGFFVLLFGISIDIA 217
           G      E+ L+ FT   V+LFG S+ +A
Sbjct: 187 GLDKYGAEAFLVNFTALIVVLFGASVVVA 215

BLAST of Csor.00g291550 vs. ExPASy Swiss-Prot
Match: Q7XMK3 (Probable ascorbate-specific transmembrane electron transporter 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0533500 PE=2 SV=2)

HSP 1 Score: 167.9 bits (424), Expect = 1.3e-40
Identity = 81/209 (38.76%), Postives = 129/209 (61.72%), Query Frame = 0

Query: 13  ASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFMLLGFVIVSGEAM 72
           A+    +AH   + A ++ LVW + +RGGLA+++ + +L+FN HP  ML+G++I+  EA+
Sbjct: 9   AAPFTYVAHALAVAAAVMVLVWCISFRGGLAFEADNKNLIFNVHPVLMLIGYIILGSEAI 68

Query: 73  MAYKTV-RADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMFSLHSWIGIVTF 132
           M YK   + +  T K++H+ LH +AI +G VGI  +FKFH+   + +++SLHSW+GI T 
Sbjct: 69  MIYKIFPKLNHDTTKLIHLILHAIAIVLGAVGIYCAFKFHNESGIANLYSLHSWLGIGTI 128

Query: 133 CLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTGLSQKATSSTLT 192
            LYG+QW+ G   F +PGA    +   LPWH+  G  + ++ + TA  GL +K T    +
Sbjct: 129 SLYGIQWIFGFVAFFYPGAAPHVRRGALPWHVLFGLFVYVLTLATAELGLLEKLTFLQSS 188

Query: 193 G----NHESRLLKFTGFFVLLFGISIDIA 217
           G      E+ L+ FTG  V LFG ++ +A
Sbjct: 189 GLDKYGAEAFLVNFTGLVVALFGAAVVVA 217

BLAST of Csor.00g291550 vs. ExPASy Swiss-Prot
Match: Q8L856 (Transmembrane ascorbate ferrireductase 1 OS=Arabidopsis thaliana OX=3702 GN=CYB561A PE=1 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.3e-40
Identity = 81/205 (39.51%), Postives = 125/205 (60.98%), Query Frame = 0

Query: 10  RHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFMLLGFVIVSG 69
           R  A A+  +AH   + A I+ LVW + YRGGLA+++ + +L+FN HP  ML+GF+I+ G
Sbjct: 4   RINAMAVTFVAHALAVIAAIMVLVWSISYRGGLAWEATNKNLIFNLHPVLMLIGFIILGG 63

Query: 70  EAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMFSLHSWIGIV 129
           EA+++YK++  +K  +K++H+ LH +A+ +GI GI A+FK H+   + +++SLHSWIGI 
Sbjct: 64  EAIISYKSLPLEKPVKKLIHLILHAIALALGIFGICAAFKNHNESHIPNLYSLHSWIGIG 123

Query: 130 TFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTGLSQKATSST 189
              LYG QW+     F FPG  +  K+ LLPWH   G  + ++ +  A  G  +K T   
Sbjct: 124 VISLYGFQWVYSFIVFFFPGGSTNLKSGLLPWHAMLGLFVYILAVGNAALGFLEKLTFLE 183

Query: 190 LTG----NHESRLLKFTGFFVLLFG 211
             G      E+ L+ FT    +LFG
Sbjct: 184 NGGLDKYGSEAFLINFTAIITILFG 208

BLAST of Csor.00g291550 vs. ExPASy Swiss-Prot
Match: C4IYS8 (Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 1.7e-40
Identity = 82/209 (39.23%), Postives = 129/209 (61.72%), Query Frame = 0

Query: 13  ASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFMLLGFVIVSGEAM 72
           A+     AH   + A  + LVW +++RGGLA ++ + +L+FN HP  ML+G++I+ GEA+
Sbjct: 9   AAPFTYAAHALAVAAAAMVLVWAIYFRGGLAIEATNKNLIFNVHPVLMLIGYIIIGGEAI 68

Query: 73  MAYKTV-RADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMFSLHSWIGIVTF 132
           M Y+ +  ++  T K++H+ LH +A+ +G VGI  +FK H+   + +++SLHSWIGI T 
Sbjct: 69  MVYRVLPTSNHETNKLIHLVLHGIALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIGTI 128

Query: 133 CLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTGLSQKATSSTLT 192
            LYG+QW++G  TF FPGA    K  +LPWHI  G  + ++ +  A  G  +K T    +
Sbjct: 129 TLYGIQWIVGFVTFFFPGAAPNVKKGVLPWHILFGLFVYILALANAELGFLEKLTFLESS 188

Query: 193 G----NHESRLLKFTGFFVLLFGISIDIA 217
           G      E+ L+ FT   V+LFG S+ +A
Sbjct: 189 GLDKYGTEAFLVNFTALVVVLFGASVVVA 217

BLAST of Csor.00g291550 vs. NCBI nr
Match: KAG6601895.1 (putative transmembrane ascorbate ferrireductase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 464 bits (1193), Expect = 8.31e-165
Identity = 229/229 (100.00%), Postives = 229/229 (100.00%), Query Frame = 0

Query: 1   MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 60
           MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM
Sbjct: 1   MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 60

Query: 61  LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF 120
           LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF
Sbjct: 61  LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF 120

Query: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG 180
           SLHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG
Sbjct: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG 180

Query: 181 LSQKATSSTLTGNHESRLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT 229
           LSQKATSSTLTGNHESRLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT
Sbjct: 181 LSQKATSSTLTGNHESRLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT 229

BLAST of Csor.00g291550 vs. NCBI nr
Match: KAG7032592.1 (putative transmembrane ascorbate ferrireductase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 462 bits (1190), Expect = 2.38e-164
Identity = 228/229 (99.56%), Postives = 229/229 (100.00%), Query Frame = 0

Query: 1   MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 60
           MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM
Sbjct: 1   MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 60

Query: 61  LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF 120
           LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF
Sbjct: 61  LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF 120

Query: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG 180
           SLHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG
Sbjct: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG 180

Query: 181 LSQKATSSTLTGNHESRLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT 229
           LSQKATSSTLTGNHE+RLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT
Sbjct: 181 LSQKATSSTLTGNHEARLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT 229

BLAST of Csor.00g291550 vs. NCBI nr
Match: XP_022961824.1 (probable transmembrane ascorbate ferrireductase 3 [Cucurbita moschata])

HSP 1 Score: 462 bits (1188), Expect = 4.81e-164
Identity = 227/229 (99.13%), Postives = 229/229 (100.00%), Query Frame = 0

Query: 1   MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 60
           MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM
Sbjct: 1   MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 60

Query: 61  LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF 120
           LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF
Sbjct: 61  LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF 120

Query: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG 180
           S+HSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG
Sbjct: 121 SIHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG 180

Query: 181 LSQKATSSTLTGNHESRLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT 229
           LSQKATSSTLTGNHE+RLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT
Sbjct: 181 LSQKATSSTLTGNHEARLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT 229

BLAST of Csor.00g291550 vs. NCBI nr
Match: XP_023521256.1 (probable transmembrane ascorbate ferrireductase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 448 bits (1152), Expect = 2.29e-158
Identity = 222/229 (96.94%), Postives = 225/229 (98.25%), Query Frame = 0

Query: 1   MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 60
           MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM
Sbjct: 13  MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 72

Query: 61  LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF 120
           LLGFVIVSGEA+MAYKTVRADKMT KIVHMALHFVAICMGIVGINA+FKFHDMIKLEHMF
Sbjct: 73  LLGFVIVSGEAIMAYKTVRADKMTTKIVHMALHFVAICMGIVGINAAFKFHDMIKLEHMF 132

Query: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG 180
           SLHSWIGIVTFCLYGLQWLLGLCTFLF GA  ETKARLLPWHISGGRALLLMIICTALTG
Sbjct: 133 SLHSWIGIVTFCLYGLQWLLGLCTFLFTGATRETKARLLPWHISGGRALLLMIICTALTG 192

Query: 181 LSQKATSSTLTGNHESRLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT 229
           LSQKATSSTLTGN+ESRLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT
Sbjct: 193 LSQKATSSTLTGNNESRLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT 241

BLAST of Csor.00g291550 vs. NCBI nr
Match: XP_022991076.1 (probable transmembrane ascorbate ferrireductase 3 [Cucurbita maxima])

HSP 1 Score: 442 bits (1138), Expect = 2.02e-156
Identity = 218/229 (95.20%), Postives = 221/229 (96.51%), Query Frame = 0

Query: 1   MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 60
           MDTV+RPGFRHTASAIA LAHIFGLTACILTLVWLLHYRGGLAYDSRSA+LVFNCHPFFM
Sbjct: 1   MDTVDRPGFRHTASAIAVLAHIFGLTACILTLVWLLHYRGGLAYDSRSAELVFNCHPFFM 60

Query: 61  LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF 120
           LLGFV VSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGI GINASFKFHDMIKLEH+F
Sbjct: 61  LLGFVFVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIAGINASFKFHDMIKLEHVF 120

Query: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG 180
           SLHSWIGIVTFCLYGLQWLLGLCTFLFPGA  ETKARLLPWHISGGRALLLMIICTALTG
Sbjct: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGASRETKARLLPWHISGGRALLLMIICTALTG 180

Query: 181 LSQKATSSTLTGNHESRLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT 229
           LSQKATSSTLTGNHESRLL FTGFFVLLFGISIDIAIGFDRFRH D TT
Sbjct: 181 LSQKATSSTLTGNHESRLLNFTGFFVLLFGISIDIAIGFDRFRHADLTT 229

BLAST of Csor.00g291550 vs. ExPASy TrEMBL
Match: A0A6J1HF52 (probable transmembrane ascorbate ferrireductase 3 OS=Cucurbita moschata OX=3662 GN=LOC111462470 PE=4 SV=1)

HSP 1 Score: 462 bits (1188), Expect = 2.33e-164
Identity = 227/229 (99.13%), Postives = 229/229 (100.00%), Query Frame = 0

Query: 1   MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 60
           MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM
Sbjct: 1   MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 60

Query: 61  LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF 120
           LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF
Sbjct: 61  LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF 120

Query: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG 180
           S+HSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG
Sbjct: 121 SIHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG 180

Query: 181 LSQKATSSTLTGNHESRLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT 229
           LSQKATSSTLTGNHE+RLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT
Sbjct: 181 LSQKATSSTLTGNHEARLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT 229

BLAST of Csor.00g291550 vs. ExPASy TrEMBL
Match: A0A6J1JPQ6 (probable transmembrane ascorbate ferrireductase 3 OS=Cucurbita maxima OX=3661 GN=LOC111487782 PE=4 SV=1)

HSP 1 Score: 442 bits (1138), Expect = 9.79e-157
Identity = 218/229 (95.20%), Postives = 221/229 (96.51%), Query Frame = 0

Query: 1   MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 60
           MDTV+RPGFRHTASAIA LAHIFGLTACILTLVWLLHYRGGLAYDSRSA+LVFNCHPFFM
Sbjct: 1   MDTVDRPGFRHTASAIAVLAHIFGLTACILTLVWLLHYRGGLAYDSRSAELVFNCHPFFM 60

Query: 61  LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF 120
           LLGFV VSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGI GINASFKFHDMIKLEH+F
Sbjct: 61  LLGFVFVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIAGINASFKFHDMIKLEHVF 120

Query: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG 180
           SLHSWIGIVTFCLYGLQWLLGLCTFLFPGA  ETKARLLPWHISGGRALLLMIICTALTG
Sbjct: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGASRETKARLLPWHISGGRALLLMIICTALTG 180

Query: 181 LSQKATSSTLTGNHESRLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT 229
           LSQKATSSTLTGNHESRLL FTGFFVLLFGISIDIAIGFDRFRH D TT
Sbjct: 181 LSQKATSSTLTGNHESRLLNFTGFFVLLFGISIDIAIGFDRFRHADLTT 229

BLAST of Csor.00g291550 vs. ExPASy TrEMBL
Match: A0A0A0KPB5 (Cytochrome b561 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G548140 PE=4 SV=1)

HSP 1 Score: 378 bits (970), Expect = 3.94e-131
Identity = 183/228 (80.26%), Postives = 201/228 (88.16%), Query Frame = 0

Query: 1   MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 60
           MD + R  FRHTAS I   AHIFGLTACILTLVWLLHYRGGLAYDS +++LVFNCHPFFM
Sbjct: 1   MDPMERANFRHTASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFM 60

Query: 61  LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF 120
           LLGFV +SGEAMMAY+TVRA+K T+KIVHMALH VAICMGI+GINA+FKFHD + LE M+
Sbjct: 61  LLGFVFMSGEAMMAYRTVRAEKRTKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMY 120

Query: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG 180
           SLHSWIGIVTFCLYGLQW++GL TFLFPGA  ETK RLLPWHI+GGRALLLMIICTALTG
Sbjct: 121 SLHSWIGIVTFCLYGLQWVVGLFTFLFPGADRETKTRLLPWHITGGRALLLMIICTALTG 180

Query: 181 LSQKATSSTLTGNHESRLLKFTGFFVLLFGISIDIAIGFDRFRHVDPT 228
           LS+KATS  LT +HESRLL FT FFVLLFGISIDI+IGF RF HVD T
Sbjct: 181 LSEKATSLRLTSSHESRLLNFTAFFVLLFGISIDISIGFARFMHVDHT 228

BLAST of Csor.00g291550 vs. ExPASy TrEMBL
Match: A0A1S3CSD9 (probable transmembrane ascorbate ferrireductase 3 OS=Cucumis melo OX=3656 GN=LOC103504338 PE=4 SV=1)

HSP 1 Score: 373 bits (958), Expect = 2.65e-129
Identity = 182/228 (79.82%), Postives = 198/228 (86.84%), Query Frame = 0

Query: 1   MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 60
           MD + R  FRHTAS IA  AHI GLTACIL LVWLLHYRGGLAYDS  A+LVFNCHPFFM
Sbjct: 1   MDPMERANFRHTASTIAVFAHISGLTACILMLVWLLHYRGGLAYDSPDANLVFNCHPFFM 60

Query: 61  LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF 120
           LLGFV +SGEAMMAY+TVRA+K+T+KIVHM LH VAICMGI+GINA+FKFHD + LE M+
Sbjct: 61  LLGFVFMSGEAMMAYRTVRAEKITKKIVHMGLHLVAICMGIIGINAAFKFHDQVNLEDMY 120

Query: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG 180
           SLHSWIGIVTFCLYGLQWLLGL TFLFPGA + TK RLLPWHISGGRALL+MIICTALTG
Sbjct: 121 SLHSWIGIVTFCLYGLQWLLGLFTFLFPGADAMTKTRLLPWHISGGRALLMMIICTALTG 180

Query: 181 LSQKATSSTLTGNHESRLLKFTGFFVLLFGISIDIAIGFDRFRHVDPT 228
           LS+KATS  LTG+HESRL  FT FFVLLFGISIDI+I F RF HVD T
Sbjct: 181 LSEKATSLRLTGSHESRLFNFTAFFVLLFGISIDISISFARFMHVDHT 228

BLAST of Csor.00g291550 vs. ExPASy TrEMBL
Match: A0A6J1CYG4 (probable transmembrane ascorbate ferrireductase 3 OS=Momordica charantia OX=3673 GN=LOC111015617 PE=4 SV=1)

HSP 1 Score: 365 bits (937), Expect = 3.90e-126
Identity = 177/219 (80.82%), Postives = 192/219 (87.67%), Query Frame = 0

Query: 1   MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 60
           MDT+ R  FRHTASA+  +AHIFG+TACIL LVWLLHYRGGL YDS + +LVFNCHPFFM
Sbjct: 1   MDTMERASFRHTASAVTIMAHIFGVTACILLLVWLLHYRGGLGYDSPNPNLVFNCHPFFM 60

Query: 61  LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF 120
           LLGFV VSGEAMMA+KTVRADKM RKI HM LH VAICMGI GINA+FKFHD++ LE MF
Sbjct: 61  LLGFVFVSGEAMMAFKTVRADKMARKITHMGLHLVAICMGIAGINAAFKFHDLVNLEDMF 120

Query: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG 180
           SLHSWIGIVTF LYGLQW+LGLCTFLFPGA + TKA+LLPWHISGGRALLLMIICTALTG
Sbjct: 121 SLHSWIGIVTFSLYGLQWVLGLCTFLFPGAEASTKAKLLPWHISGGRALLLMIICTALTG 180

Query: 181 LSQKATSSTLTGNHESRLLKFTGFFVLLFGISIDIAIGF 219
           LSQKATS  L  NHESRLL FT FFVLLFGISI+I +GF
Sbjct: 181 LSQKATSLKLVANHESRLLNFTAFFVLLFGISINIVVGF 219

BLAST of Csor.00g291550 vs. TAIR 10
Match: AT1G14730.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 220.3 bits (560), Expect = 1.6e-57
Identity = 106/218 (48.62%), Postives = 143/218 (65.60%), Query Frame = 0

Query: 5   NRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFMLLGF 64
           +R   +  +S    +AH FG+ A +L L+WLLHYR G+ Y S +   V N HPF M  GF
Sbjct: 6   DRTTLKRHSSLSTLVAHFFGILAVVLMLIWLLHYREGIEYGSDNPLKVLNVHPFLMYCGF 65

Query: 65  VIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMFSLHS 124
           + + G+AMM YKT  A    +K+VH  LH + + +GIVGI A+F+FHD + L+ M SLHS
Sbjct: 66  LFLVGQAMMTYKTAYASHQVQKMVHGGLHLIGLVLGIVGICAAFRFHDKVNLKDMVSLHS 125

Query: 125 WIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTGLSQK 184
           WIG+ TF L G+QWL G  TFL P + S T+ R++PWH+ GGRALL M I  ALTGL Q+
Sbjct: 126 WIGLTTFILLGVQWLFGAFTFLAPQSSSGTRTRMMPWHVLGGRALLYMGIVAALTGLMQR 185

Query: 185 ATSSTLTGNHESRLLKFTGFFVLLFGISIDIAIGFDRF 223
           AT    + N ESRL+ F G  +LLFG+S+D ++   R+
Sbjct: 186 ATMLGQSTNAESRLINFLGLAILLFGVSVDFSVALGRY 223

BLAST of Csor.00g291550 vs. TAIR 10
Match: AT4G25570.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 167.9 bits (424), Expect = 9.3e-42
Identity = 81/205 (39.51%), Postives = 125/205 (60.98%), Query Frame = 0

Query: 10  RHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFMLLGFVIVSG 69
           R  A A+  +AH   + A I+ LVW + YRGGLA+++ + +L+FN HP  ML+GF+I+ G
Sbjct: 4   RINAMAVTFVAHALAVIAAIMVLVWSISYRGGLAWEATNKNLIFNLHPVLMLIGFIILGG 63

Query: 70  EAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMFSLHSWIGIV 129
           EA+++YK++  +K  +K++H+ LH +A+ +GI GI A+FK H+   + +++SLHSWIGI 
Sbjct: 64  EAIISYKSLPLEKPVKKLIHLILHAIALALGIFGICAAFKNHNESHIPNLYSLHSWIGIG 123

Query: 130 TFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTGLSQKATSST 189
              LYG QW+     F FPG  +  K+ LLPWH   G  + ++ +  A  G  +K T   
Sbjct: 124 VISLYGFQWVYSFIVFFFPGGSTNLKSGLLPWHAMLGLFVYILAVGNAALGFLEKLTFLE 183

Query: 190 LTG----NHESRLLKFTGFFVLLFG 211
             G      E+ L+ FT    +LFG
Sbjct: 184 NGGLDKYGSEAFLINFTAIITILFG 208

BLAST of Csor.00g291550 vs. TAIR 10
Match: AT5G38630.1 (cytochrome B561-1 )

HSP 1 Score: 150.6 bits (379), Expect = 1.5e-36
Identity = 71/200 (35.50%), Postives = 117/200 (58.50%), Query Frame = 0

Query: 16  IAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFMLLGFVIVSGEAMMAY 75
           I  +  + G     L L W +HYRGGLA  S + D +FN HP  M++G ++ +GEAM+AY
Sbjct: 11  IFMVVRVLGFIIAALVLTWTVHYRGGLALSSDNKDHIFNVHPVMMVIGLILFNGEAMLAY 70

Query: 76  KTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMFSLHSWIGIVTFCLYG 135
           K+V+  K  +K+VH+ L   A  + ++G+ A+ KFH    +E+ +SLHSW+G+    L+ 
Sbjct: 71  KSVQGTKNLKKLVHLTLQLTAFILSLIGVWAALKFHIDKGIENFYSLHSWLGLACLFLFA 130

Query: 136 LQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTGLSQKAT----SSTLT 195
            QW  G  T+ +PG    ++A L+PWH+  G ++  + + TA TG+ +K T    +  +T
Sbjct: 131 FQWAAGFVTYWYPGGSRNSRASLMPWHVFLGISIYALALVTATTGILEKVTFLQVNQVIT 190

Query: 196 G-NHESRLLKFTGFFVLLFG 211
             + E+ L+   G  +L+ G
Sbjct: 191 RYSTEAMLVNTMGVLILILG 210

BLAST of Csor.00g291550 vs. TAIR 10
Match: AT1G26100.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 96.3 bits (238), Expect = 3.4e-20
Identity = 54/173 (31.21%), Postives = 90/173 (52.02%), Query Frame = 0

Query: 15  AIAALAHIFGLTACILTLVWLLHYRG-GLAYDSRSADLVFNCHPFFMLLGFVIVSGEAMM 74
           ++   A + GL   +  L W L     GL+Y +         HP  M++GF++VSGEA++
Sbjct: 10  SLVLFARLSGLVVAVSVLYWALFLPNLGLSYST--------LHPLLMVIGFILVSGEAIL 69

Query: 75  AYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMFSLHSWIGIVTFCL 134
            ++ +   + T+K VH+ L  +A+   + GI   F +   +   + +SLHSW+G+++  L
Sbjct: 70  IHRWLPGSRKTKKAVHLWLQGMALASAVFGIWTKFHYQRGV-FANFYSLHSWMGLLSVSL 129

Query: 135 YGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTGLSQKAT 187
           +  QW+ G  +F   G    T+   LPWH+  G     + I TA TGL +K T
Sbjct: 130 FAAQWVTGFMSFWHRGEVRTTRTTFLPWHVFLGLYTYGLAIATAETGLLEKLT 173

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q67ZF62.2e-5648.62Probable transmembrane ascorbate ferrireductase 3 OS=Arabidopsis thaliana OX=370... [more]
A3A9H69.1e-4239.71Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa... [more]
Q7XMK31.3e-4038.76Probable ascorbate-specific transmembrane electron transporter 2 OS=Oryza sativa... [more]
Q8L8561.3e-4039.51Transmembrane ascorbate ferrireductase 1 OS=Arabidopsis thaliana OX=3702 GN=CYB5... [more]
C4IYS81.7e-4039.23Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2... [more]
Match NameE-valueIdentityDescription
KAG6601895.18.31e-165100.00putative transmembrane ascorbate ferrireductase 3, partial [Cucurbita argyrosper... [more]
KAG7032592.12.38e-16499.56putative transmembrane ascorbate ferrireductase 3, partial [Cucurbita argyrosper... [more]
XP_022961824.14.81e-16499.13probable transmembrane ascorbate ferrireductase 3 [Cucurbita moschata][more]
XP_023521256.12.29e-15896.94probable transmembrane ascorbate ferrireductase 3 [Cucurbita pepo subsp. pepo][more]
XP_022991076.12.02e-15695.20probable transmembrane ascorbate ferrireductase 3 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1HF522.33e-16499.13probable transmembrane ascorbate ferrireductase 3 OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1JPQ69.79e-15795.20probable transmembrane ascorbate ferrireductase 3 OS=Cucurbita maxima OX=3661 GN... [more]
A0A0A0KPB53.94e-13180.26Cytochrome b561 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G54... [more]
A0A1S3CSD92.65e-12979.82probable transmembrane ascorbate ferrireductase 3 OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A6J1CYG43.90e-12680.82probable transmembrane ascorbate ferrireductase 3 OS=Momordica charantia OX=3673... [more]
Match NameE-valueIdentityDescription
AT1G14730.11.6e-5748.62Cytochrome b561/ferric reductase transmembrane protein family [more]
AT4G25570.19.3e-4239.51Cytochrome b561/ferric reductase transmembrane protein family [more]
AT5G38630.11.5e-3635.50cytochrome B561-1 [more]
AT1G26100.13.4e-2031.21Cytochrome b561/ferric reductase transmembrane protein family [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006593Cytochrome b561/ferric reductase transmembraneSMARTSM00665561_7coord: 55..181
e-value: 2.9E-43
score: 159.7
IPR006593Cytochrome b561/ferric reductase transmembranePFAMPF03188Cytochrom_B561coord: 56..186
e-value: 3.3E-37
score: 127.7
IPR006593Cytochrome b561/ferric reductase transmembranePROSITEPS50939CYTOCHROME_B561coord: 19..218
score: 42.566673
NoneNo IPR availableGENE3D1.20.120.1770coord: 10..220
e-value: 2.2E-64
score: 218.7
NoneNo IPR availablePANTHERPTHR10106:SF15TRANSMEMBRANE ASCORBATE FERRIREDUCTASE 3-RELATEDcoord: 4..220
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..28
score: 5.0
NoneNo IPR availableCDDcd08766Cyt_b561_ACYB-1_likecoord: 46..186
e-value: 1.22445E-61
score: 187.899
IPR043205Cytochrome b561/Cytochrome b reductase 1-likePANTHERPTHR10106CYTOCHROME B561-RELATEDcoord: 4..220

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g291550.m01Csor.00g291550.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016491 oxidoreductase activity