Csor.00g290520 (gene) Silver-seed gourd (wild; sororia) v1

BLAST of Csor.00g290520 vs. ExPASy Swiss-Prot
Match: M4IQQ5 (2-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 6.5e-227
Identity = 369/549 (67.21%), Postives = 452/549 (82.33%), Query Frame = 0

Query: 1   MEDLKPRTPNCAPLTPIGFLERAATIYRDSPSIIYGHTTYTWSETHHRCLQLASSLSSSG 60
           MEDLKPR  + +PLTP+GFLERAAT+Y D  S++Y   +YTWS+TH RCL LASS++S G
Sbjct: 1   MEDLKPRPASSSPLTPLGFLERAATVYGDCTSVVYDAVSYTWSQTHRRCLCLASSIASLG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVDQA 120
           I+ GHVVSV++PN P MYELHFAVPMAGAILN +N RLDARTIS LL HSESKL+FVD  
Sbjct: 61  IENGHVVSVLAPNVPQMYELHFAVPMAGAILNAVNLRLDARTISILLHHSESKLIFVDHL 120

Query: 121 YCALISDALALFPPETDPPSLVLIDDAAAAEESLSSSISIRTDFVDSYEEMIQKGDSKFQ 180
              LI +A+ALFP +   P LV + D    E    +S  +  +F  SY+++I +GD  F+
Sbjct: 121 SRDLILEAIALFPKQAPVPRLVFMAD----ESESGNSSELGKEFFCSYKDLIDRGDPDFK 180

Query: 181 WIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMFH 240
           W+ P SEWDP++LNYTSGTTSSPKGVVHCHRG+FI   DSL++WGVPK  VYLWTLPMFH
Sbjct: 181 WVMPKSEWDPMILNYTSGTTSSPKGVVHCHRGIFIMTVDSLIDWGVPKQPVYLWTLPMFH 240

Query: 241 INGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNQPLD 300
            NGWS+ WG+AAVGGTN+C+RKFD+ +I+ +I+RH VTHMC APVVLNML+N+  ++PL 
Sbjct: 241 ANGWSYPWGMAAVGGTNICLRKFDSEIIYDMIKRHGVTHMCGAPVVLNMLSNAPGSEPLK 300

Query: 301 RPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQARL 360
             V+I+T GAPPP+AVLFR ESLGF V+HGYGLTETAG+V+ C+WK EWN LPATE+ARL
Sbjct: 301 TTVQIMTAGAPPPSAVLFRTESLGFAVSHGYGLTETAGLVVSCAWKKEWNHLPATERARL 360

Query: 361 KARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMTDK 420
           K+RQGV T     +DVVD  TG +VK+DG ++GE+V+RGGS+M+GY KD E TAKSMT  
Sbjct: 361 KSRQGVGTVMQTKIDVVDPVTGAAVKRDGSTLGEVVLRGGSVMLGYLKDPEGTAKSMTAD 420

Query: 421 GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARADE 480
           GWF TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LY++P + EAAVVAR DE
Sbjct: 421 GWFYTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYSHPDILEAAVVARPDE 480

Query: 481 FWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKYV 540
           FWGETPCAFVSL++GLT+KPTE+EI++YCR KLPR+MVPKTVVF  ELPKTSTGK++K++
Sbjct: 481 FWGETPCAFVSLKKGLTKKPTEKEIVEYCRSKLPRYMVPKTVVFKEELPKTSTGKVQKFI 540

Query: 541 LKEIAMSIG 550
           L+++A  +G
Sbjct: 541 LRDMARGMG 545

BLAST of Csor.00g290520 vs. ExPASy Swiss-Prot
Match: M4ISH2 (Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1)

HSP 1 Score: 755.0 bits (1948), Expect = 6.1e-217
Identity = 362/551 (65.70%), Postives = 447/551 (81.13%), Query Frame = 0

Query: 1   MEDLKPRTPNCAPLTPIGFLERAATIYRDSPSIIYGHTTYTWSETHHRCLQLASSLSSS- 60
           ME+LKPR  N  PLTP+GFLERAAT+Y D  S++Y   +YTWS+TH RCL++AS + S  
Sbjct: 1   MEELKPRFANSCPLTPLGFLERAATVYGDCISVVYDDLSYTWSQTHTRCLRVASCIESCL 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVDQ 120
           G+++G VVSV++PN P MYEL+FAVPMAG +LN INTRL+A TIS +L HSESKLVFVDQ
Sbjct: 61  GVKKGQVVSVVAPNVPAMYELNFAVPMAGVVLNNINTRLNAVTISVMLRHSESKLVFVDQ 120

Query: 121 AYCALISDALALFPPETDPPSLVLIDDAAAAEESLSSSISIRTDFVDSYEEMIQKGDSK- 180
               L+ DA++LFP  T  P LVLI D    E   + ++  R  FV  YE++++KGD K 
Sbjct: 121 LSVRLVLDAVSLFPKNTPTPLLVLIADNITGE---NLTVEDREHFVCCYEDLVEKGDDKT 180

Query: 181 FQWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPM 240
           F+W+RPISEWDPIVLNYTSGTTSSPKGVVH HR VF+   DSL++WGVPK  VYLWTLPM
Sbjct: 181 FKWVRPISEWDPIVLNYTSGTTSSPKGVVHSHRSVFVVTLDSLIDWGVPKQPVYLWTLPM 240

Query: 241 FHINGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNQP 300
           FH NGW ++WGIAAVGGTN+C+R+FD  +IF+LIRRH VTHMCAAP+VLNML+NS + +P
Sbjct: 241 FHANGWGYTWGIAAVGGTNICLRRFDGEIIFNLIRRHRVTHMCAAPIVLNMLSNSPNAEP 300

Query: 301 LDRPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQA 360
           L  PV ++TGGAPPPAAVL R ESLGF ++HGYG+TE  G+V+ C+WK EWNRLPATEQA
Sbjct: 301 LPNPVHVMTGGAPPPAAVLLRTESLGFVISHGYGMTEMLGVVVSCAWKREWNRLPATEQA 360

Query: 361 RLKARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMT 420
           RLK+RQGVRTAA+  VDVVD  +G SVK+DG+++GEIV++G SIM+GY K+  ATAK + 
Sbjct: 361 RLKSRQGVRTAAMMEVDVVDPNSGVSVKRDGLTMGEIVLKGSSIMLGYLKNSAATAKCIR 420

Query: 421 DKGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARA 480
             GWF TGD+ VMHPDGYLEIKDRSKDVIISGGEN+SSVEVES LY++P+V+EAAVVA  
Sbjct: 421 ADGWFYTGDMAVMHPDGYLEIKDRSKDVIISGGENVSSVEVESALYSHPAVDEAAVVACP 480

Query: 481 DEFWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKK 540
           DE+WGETP AFV+L++G+  +PTE+EI++YCR KL  FMVPK VVF  +LPKTSTGKI+K
Sbjct: 481 DEYWGETPFAFVTLKKGMRVRPTEKEILEYCREKLAHFMVPKVVVFRDKLPKTSTGKIQK 540

Query: 541 YVLKEIAMSIG 550
           + LKEI  ++G
Sbjct: 541 FTLKEIVKTMG 548

BLAST of Csor.00g290520 vs. ExPASy Swiss-Prot
Match: Q9FFE6 (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE5 PE=1 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 2.0e-215
Identity = 365/550 (66.36%), Postives = 446/550 (81.09%), Query Frame = 0

Query: 1   MEDLKPRTPNCAPLTPIGFLERAATIYRDSPSIIYG-HTTYTWSETHHRCLQLASSLSSS 60
           ME +KP   N  PLTPIGFLERAAT+Y D  SI+YG +T YTW ET+ RCL++ASSLSS 
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVDQ 120
           GI R  VVSV+SPNTP MYEL FAVPM+GAILN INTRLDART+S LL H  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 AYCALISDALALFPPETDPPSLVLIDDAAAAEESLSSSISIRTDFVDSYEEMIQKGDSKF 180
               L  +A+++    TDPP LV I D    EE   + ++ RT F  +Y+++I +GD  F
Sbjct: 121 FSVDLAVEAISMM--TTDPPILVFIAD--KEEEGGDADVADRTKFSYTYDDLIHRGDLDF 180

Query: 181 QWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMF 240
           +WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRG+F+ + DSL++W VPKN VYLWTLP+F
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIF 240

Query: 241 HINGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNQPL 300
           H NGWS+ WGIAAVGGTNVC+RKFDA LI+ LIR H VTHMC APVVLNML+ + + QPL
Sbjct: 241 HANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPL 300

Query: 301 DRPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQAR 360
           +RPV ILT GAPPPAAVL R ES+GF ++HGYGLTETAG+ + C+WK +WNRLPA+++AR
Sbjct: 301 NRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRAR 360

Query: 361 LKARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMTD 420
           LKARQGVRT     +DVVD E+G+SV+++G ++GEIV+RG SIM+GY KD   T K++ +
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKALKN 420

Query: 421 KGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARAD 480
            GWF TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYTNP+VNE AVVAR D
Sbjct: 421 -GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKY 540
            FWGETPCAFVSL+ GLT++PTE E+I+YCR K+P++MVPKTV F+ ELPKTSTGK+ K+
Sbjct: 481 VFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKF 540

Query: 541 VLKEIAMSIG 550
           VL+EIA  +G
Sbjct: 541 VLREIAKKMG 545

BLAST of Csor.00g290520 vs. ExPASy Swiss-Prot
Match: Q9FFE9 (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 SV=1)

HSP 1 Score: 738.8 bits (1906), Expect = 4.5e-212
Identity = 357/552 (64.67%), Postives = 446/552 (80.80%), Query Frame = 0

Query: 1   MEDLKPRTPNCAPLTPIGFLERAATIYRDSPSIIYG-HTTYTWSETHHRCLQLASSLSSS 60
           ME++KP   N  PLTPIGFLERAAT+Y D  SI+YG +T YTW ET+ RCL++ASSLSS 
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVDQ 120
           GI R  VVSV+SPNTP MYEL FAVPM+GAILN INTRLDART+S LL H ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 AYCALISDALALFPPETDPPSLVLIDDAAAAEESLSSSISIRTDFVDSYEEMIQKGDSKF 180
               L  +A+++    TDPP LV+I D    EE   + ++  + F  +Y+++I++GD  F
Sbjct: 121 FSVDLAVEAVSMM--TTDPPILVVIAD--KEEEGGVADVADLSKFSYTYDDLIERGDPGF 180

Query: 181 QWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMF 240
           +WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRG+F+ + DSL++W VPKN VYLWTLP+F
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIF 240

Query: 241 HINGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNQPL 300
           H NGW+  WGIAAVGGTNVC+RKFDA LI+ LIR H VTHMC APVVLNML+ + ++QPL
Sbjct: 241 HSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPL 300

Query: 301 DRPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQAR 360
           + PV ILT G+PPPA VL R ES+GF ++HGYGLTETAG+++ C+WK +WN LPA+++AR
Sbjct: 301 NHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRAR 360

Query: 361 LKARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMTD 420
           LKARQGVRT     +DVVD E+G SV+++G ++GEIV+RG S+M+GY KD   T K++ +
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKALKN 420

Query: 421 KGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARAD 480
            GWF TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYT P+VNE AVVAR D
Sbjct: 421 -GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKY 540
           EFWGETPCAFVSL+ G + KPTEEE+++YCR K+P++MVPKTV FM ELPK+STGK+ K+
Sbjct: 481 EFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKF 540

Query: 541 VLKEIAMSIGPK 552
           VL++IA  +G K
Sbjct: 541 VLRDIAKKMGDK 547

BLAST of Csor.00g290520 vs. ExPASy Swiss-Prot
Match: Q9LQS1 (Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 SV=1)

HSP 1 Score: 714.1 bits (1842), Expect = 1.2e-204
Identity = 355/550 (64.55%), Postives = 433/550 (78.73%), Query Frame = 0

Query: 1   MEDLKPRTPNCAPLTPIGFLERAATIYRDSPSIIYGHTT-YTWSETHHRCLQLASSLSSS 60
           MEDLKP   N  PLT +GFLERAAT+Y D  SI+YG++T YTW ET+HRCL +AS+LSS 
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVDQ 120
           GI R  VVSV+S NTP MYEL F+VPM+GAILN INTRLDART+S LL H ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 AYCALISDALALFPPETDPPSLVLIDDAAAAEESLSSSISIRTDFVDSYEEMIQKGDSKF 180
            Y  L  +A+ +     +PP LVLI  A   EE   + ++ R+ F   Y ++I +G+  F
Sbjct: 121 FYSDLAVEAITML---LNPPILVLI--ANEEEEEGGAEVTERSKFCYLYSDLITRGNPDF 180

Query: 181 QWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMF 240
           +WIRP SEWDPIV+NYTSGTTSSPKGVVHCHRG+F+   DSL +W VPK  VYLWTLP+F
Sbjct: 181 KWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIF 240

Query: 241 HINGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLD-NQP 300
           H NGW++ WGIAAVGGTNVC+RK  A  I+ LIR H VTHM  AP+VL +L+ S + +QP
Sbjct: 241 HANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQP 300

Query: 301 LDRPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQA 360
           L  PV  LT G+ PPA VL R ESLGF V+HGYGLTETAG+++ C+WK  WNRLPA++QA
Sbjct: 301 LKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQA 360

Query: 361 RLKARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMT 420
           +LK+RQGVRT   + +DVVD E+G+SV++DG ++GEIV+RG SIM+GY K+   T  S  
Sbjct: 361 QLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSFK 420

Query: 421 DKGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARA 480
           + GWF TGD+GV+H DGYLEIKDRSKDVIISGGEN+SSVEVE+VLYTNP+VNEAAVVAR 
Sbjct: 421 N-GWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARP 480

Query: 481 DEFWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKK 540
           DEFWGETPCAFVSL+ GLTRKPT++EII+YC+ K+PR+M PKTV F+ ELPKTSTGKI K
Sbjct: 481 DEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIK 540

Query: 541 YVLKEIAMSI 549
            +LKEIA ++
Sbjct: 541 SLLKEIAKNM 544

BLAST of Csor.00g290520 vs. NCBI nr
Match: KAG6601998.1 (putative acyl-activating enzyme 5, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1122 bits (2903), Expect = 0.0
Identity = 555/555 (100.00%), Postives = 555/555 (100.00%), Query Frame = 0

Query: 1   MEDLKPRTPNCAPLTPIGFLERAATIYRDSPSIIYGHTTYTWSETHHRCLQLASSLSSSG 60
           MEDLKPRTPNCAPLTPIGFLERAATIYRDSPSIIYGHTTYTWSETHHRCLQLASSLSSSG
Sbjct: 1   MEDLKPRTPNCAPLTPIGFLERAATIYRDSPSIIYGHTTYTWSETHHRCLQLASSLSSSG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVDQA
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVDQA 120

Query: 121 YCALISDALALFPPETDPPSLVLIDDAAAAEESLSSSISIRTDFVDSYEEMIQKGDSKFQ 180
           YCALISDALALFPPETDPPSLVLIDDAAAAEESLSSSISIRTDFVDSYEEMIQKGDSKFQ
Sbjct: 121 YCALISDALALFPPETDPPSLVLIDDAAAAEESLSSSISIRTDFVDSYEEMIQKGDSKFQ 180

Query: 181 WIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMFH 240
           WIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMFH
Sbjct: 181 WIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMFH 240

Query: 241 INGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNQPLD 300
           INGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNQPLD
Sbjct: 241 INGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNQPLD 300

Query: 301 RPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQARL 360
           RPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQARL
Sbjct: 301 RPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQARL 360

Query: 361 KARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMTDK 420
           KARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMTDK
Sbjct: 361 KARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMTDK 420

Query: 421 GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARADE 480
           GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARADE
Sbjct: 421 GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARADE 480

Query: 481 FWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKYV 540
           FWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKYV
Sbjct: 481 FWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKYV 540

Query: 541 LKEIAMSIGPKPSRL 555
           LKEIAMSIGPKPSRL
Sbjct: 541 LKEIAMSIGPKPSRL 555

BLAST of Csor.00g290520 vs. NCBI nr
Match: XP_022962703.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata])

HSP 1 Score: 1083 bits (2802), Expect = 0.0
Identity = 541/556 (97.30%), Postives = 545/556 (98.02%), Query Frame = 0

Query: 1   MEDLKPRTPNCAPLTPIGFLERAATIYRDSPSIIYGHTTYTWSETHHRCLQLASSLSSSG 60
           MEDLKPRTPN APLTPIGFLERAATIY DSPSIIYGHTTYTWSETHHRCLQLASSLSSSG
Sbjct: 39  MEDLKPRTPNSAPLTPIGFLERAATIYGDSPSIIYGHTTYTWSETHHRCLQLASSLSSSG 98

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFV QA
Sbjct: 99  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVHQA 158

Query: 121 YCALISDALALFPPETDPPSLVLIDD-AAAAEESLSSSISIRTDFVDSYEEMIQKGDSKF 180
           YCALISDALALFP ET+PPSLVLIDD AAAAEESLSSS+SIR DFVDSYEEMIQKGDSKF
Sbjct: 159 YCALISDALALFPLETNPPSLVLIDDDAAAAEESLSSSVSIRIDFVDSYEEMIQKGDSKF 218

Query: 181 QWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMF 240
           QWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMF
Sbjct: 219 QWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMF 278

Query: 241 HINGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNQPL 300
           HINGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDN+ L
Sbjct: 279 HINGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSL 338

Query: 301 DRPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQAR 360
           DRPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQAR
Sbjct: 339 DRPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQAR 398

Query: 361 LKARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMTD 420
           LKARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVV GGSIMVGYWKDREATAKSMTD
Sbjct: 399 LKARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVSGGSIMVGYWKDREATAKSMTD 458

Query: 421 KGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARAD 480
           +GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVAR D
Sbjct: 459 EGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARPD 518

Query: 481 EFWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKY 540
           EFWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKY
Sbjct: 519 EFWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKY 578

Query: 541 VLKEIAMSIGPKPSRL 555
           VLKEIAMSIG K SRL
Sbjct: 579 VLKEIAMSIGSKSSRL 594

BLAST of Csor.00g290520 vs. NCBI nr
Match: XP_023554638.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1083 bits (2800), Expect = 0.0
Identity = 539/557 (96.77%), Postives = 546/557 (98.03%), Query Frame = 0

Query: 1   MEDLKPRTPNCAPLTPIGFLERAATIYRDSPSIIYGHTTYTWSETHHRCLQLASSLSSSG 60
           MEDLKPRTPN +PLTPIGFLERAATIY DSPSIIYG TTYTWSETHHRCLQLASSLSSSG
Sbjct: 39  MEDLKPRTPNSSPLTPIGFLERAATIYGDSPSIIYGDTTYTWSETHHRCLQLASSLSSSG 98

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLL HSESKLVFVDQA
Sbjct: 99  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQA 158

Query: 121 YCALISDALALFPPETDPPSLVLIDD--AAAAEESLSSSISIRTDFVDSYEEMIQKGDSK 180
           YCALI DALALFPPET+PPSLVLIDD  AAA EESLSSS+SIR DFVDSYEEMIQKGDSK
Sbjct: 159 YCALIFDALALFPPETNPPSLVLIDDDAAAAEEESLSSSVSIRIDFVDSYEEMIQKGDSK 218

Query: 181 FQWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPM 240
           FQWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPM
Sbjct: 219 FQWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPM 278

Query: 241 FHINGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNQP 300
           FHINGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLD++P
Sbjct: 279 FHINGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDDRP 338

Query: 301 LDRPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQA 360
           LDRPVKILTGGAP PAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQA
Sbjct: 339 LDRPVKILTGGAPLPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQA 398

Query: 361 RLKARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMT 420
           RLKARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMT
Sbjct: 399 RLKARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMT 458

Query: 421 DKGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARA 480
           D+GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVAR 
Sbjct: 459 DEGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARP 518

Query: 481 DEFWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKK 540
           DEFWGETPCAFVSLREGLTRKPTEEEII+YCRGKLPRFMVPKTVVFMAELPKTSTGKIKK
Sbjct: 519 DEFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGKIKK 578

Query: 541 YVLKEIAMSIGPKPSRL 555
           YVLKEIAMSIGPKPSRL
Sbjct: 579 YVLKEIAMSIGPKPSRL 595

BLAST of Csor.00g290520 vs. NCBI nr
Match: XP_022990524.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima])

HSP 1 Score: 1063 bits (2750), Expect = 0.0
Identity = 525/555 (94.59%), Postives = 539/555 (97.12%), Query Frame = 0

Query: 1   MEDLKPRTPNCAPLTPIGFLERAATIYRDSPSIIYGHTTYTWSETHHRCLQLASSLSSSG 60
           MEDLKPR PN APLTPIGFLERAATIY + PSIIYG TTYTWSETHHRCLQLASSLSSSG
Sbjct: 39  MEDLKPRPPNSAPLTPIGFLERAATIYGNFPSIIYGDTTYTWSETHHRCLQLASSLSSSG 98

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLL HSESKLVFVDQA
Sbjct: 99  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQA 158

Query: 121 YCALISDALALFPPETDPPSLVLIDDAAAAEESLSSSISIRTDFVDSYEEMIQKGDSKFQ 180
           YCALISDALALFPPET+PPSLVLIDD AAAEESLSSS+SIR DF DSYEEMIQKGD KFQ
Sbjct: 159 YCALISDALALFPPETNPPSLVLIDDDAAAEESLSSSVSIRIDFFDSYEEMIQKGDYKFQ 218

Query: 181 WIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMFH 240
           WIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTL MFH
Sbjct: 219 WIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSMFH 278

Query: 241 INGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNQPLD 300
           INGWSFSWGIAAVGGTN+CIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDN+ LD
Sbjct: 279 INGWSFSWGIAAVGGTNICIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSLD 338

Query: 301 RPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQARL 360
           RPVKILTGG+PPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPA  QARL
Sbjct: 339 RPVKILTGGSPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQARL 398

Query: 361 KARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMTDK 420
           KARQGVRTAAVAAVDVVD +TGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKS+TD+
Sbjct: 399 KARQGVRTAAVAAVDVVDSKTGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSITDE 458

Query: 421 GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARADE 480
           GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LYTNP+VNEAAVVAR DE
Sbjct: 459 GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPDE 518

Query: 481 FWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKYV 540
           FWGETPCAFVSLREGLTRKPTEEEII+YCRGKLPRFMVPKTVVF+AELPKTSTGKIKKYV
Sbjct: 519 FWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKKYV 578

Query: 541 LKEIAMSIGPKPSRL 555
           LKE+AMSIGPKPSRL
Sbjct: 579 LKEMAMSIGPKPSRL 593

BLAST of Csor.00g290520 vs. NCBI nr
Match: XP_023553079.1 (LOW QUALITY PROTEIN: probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 978 bits (2527), Expect = 0.0
Identity = 476/550 (86.55%), Postives = 510/550 (92.73%), Query Frame = 0

Query: 1   MEDLKPRTPNCAPLTPIGFLERAATIYRDSPSIIYGHTTYTWSETHHRCLQLASSLSSSG 60
           ME+LKPR  N +PLTP+GFLERAATIY DSPSIIY HTTYTWSETHHRCLQLASSLSS+G
Sbjct: 1   MEELKPRPANFSPLTPLGFLERAATIYGDSPSIIYAHTTYTWSETHHRCLQLASSLSSAG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTI+TRLDARTIS LL HSESKL+FVDQA
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTISTRLDARTISVLLCHSESKLIFVDQA 120

Query: 121 YCALISDALALFPPETDPPSLVLIDDAAAAEESLSSSISIRTDFVDSYEEMIQKGDSKFQ 180
           Y ALI DALALFPPE + P LVLI D A AEES SSS+ I  +FV SYEEMI+KGDS F+
Sbjct: 121 YYALIQDALALFPPEINRPMLVLIADDAVAEES-SSSVLIEGNFVGSYEEMIRKGDSDFR 180

Query: 181 WIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMFH 240
           WIRP SEWDPIVLNYTSGTTSSPKGVVHCHRG+FI   D LLEWGVPK S +LWTLPMFH
Sbjct: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFIVTLDMLLEWGVPKQSTFLWTLPMFH 240

Query: 241 INGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNQPLD 300
           INGWS +WG+AAVGGTN+CIRKFDAALI+SLIRRHHVTHMCAAPVVLNMLTNSLDN+PLD
Sbjct: 241 INGWSLTWGMAAVGGTNICIRKFDAALIYSLIRRHHVTHMCAAPVVLNMLTNSLDNRPLD 300

Query: 301 RPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQARL 360
           RPVKI TGGAPPPAAVL RIE+LGFDVTHGYGLTETAG+V CCSWK EWNRLPATE ARL
Sbjct: 301 RPVKIFTGGAPPPAAVLSRIETLGFDVTHGYGLTETAGIVTCCSWKAEWNRLPATEHARL 360

Query: 361 KARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMTDK 420
           KARQGVRTAA+AAVDVVDLETGKSVKQDGVSIGEIV+RGG+IM+GYWKD EATAKSMT++
Sbjct: 361 KARQGVRTAAMAAVDVVDLETGKSVKQDGVSIGEIVLRGGTIMIGYWKDGEATAKSMTEE 420

Query: 421 GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARADE 480
           GWF TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LYTNPSVNEAAVVAR DE
Sbjct: 421 GWFHTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARLDE 480

Query: 481 FWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKYV 540
           FWGETPCAFVSLREGLT+KPTEEEII+YCRGKLPRFM PKTVVFM ELPKTSTGKI+KY+
Sbjct: 481 FWGETPCAFVSLREGLTQKPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKYL 540

Query: 541 LKEIAMSIGP 550
           L+E+ MS+GP
Sbjct: 541 LREMTMSMGP 549

BLAST of Csor.00g290520 vs. ExPASy TrEMBL
Match: A0A6J1HFJ6 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111463113 PE=4 SV=1)

HSP 1 Score: 1083 bits (2802), Expect = 0.0
Identity = 541/556 (97.30%), Postives = 545/556 (98.02%), Query Frame = 0

Query: 1   MEDLKPRTPNCAPLTPIGFLERAATIYRDSPSIIYGHTTYTWSETHHRCLQLASSLSSSG 60
           MEDLKPRTPN APLTPIGFLERAATIY DSPSIIYGHTTYTWSETHHRCLQLASSLSSSG
Sbjct: 39  MEDLKPRTPNSAPLTPIGFLERAATIYGDSPSIIYGHTTYTWSETHHRCLQLASSLSSSG 98

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFV QA
Sbjct: 99  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVHQA 158

Query: 121 YCALISDALALFPPETDPPSLVLIDD-AAAAEESLSSSISIRTDFVDSYEEMIQKGDSKF 180
           YCALISDALALFP ET+PPSLVLIDD AAAAEESLSSS+SIR DFVDSYEEMIQKGDSKF
Sbjct: 159 YCALISDALALFPLETNPPSLVLIDDDAAAAEESLSSSVSIRIDFVDSYEEMIQKGDSKF 218

Query: 181 QWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMF 240
           QWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMF
Sbjct: 219 QWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMF 278

Query: 241 HINGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNQPL 300
           HINGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDN+ L
Sbjct: 279 HINGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSL 338

Query: 301 DRPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQAR 360
           DRPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQAR
Sbjct: 339 DRPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQAR 398

Query: 361 LKARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMTD 420
           LKARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVV GGSIMVGYWKDREATAKSMTD
Sbjct: 399 LKARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVSGGSIMVGYWKDREATAKSMTD 458

Query: 421 KGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARAD 480
           +GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVAR D
Sbjct: 459 EGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARPD 518

Query: 481 EFWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKY 540
           EFWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKY
Sbjct: 519 EFWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKY 578

Query: 541 VLKEIAMSIGPKPSRL 555
           VLKEIAMSIG K SRL
Sbjct: 579 VLKEIAMSIGSKSSRL 594

BLAST of Csor.00g290520 vs. ExPASy TrEMBL
Match: A0A6J1JS99 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111487376 PE=4 SV=1)

HSP 1 Score: 1063 bits (2750), Expect = 0.0
Identity = 525/555 (94.59%), Postives = 539/555 (97.12%), Query Frame = 0

Query: 1   MEDLKPRTPNCAPLTPIGFLERAATIYRDSPSIIYGHTTYTWSETHHRCLQLASSLSSSG 60
           MEDLKPR PN APLTPIGFLERAATIY + PSIIYG TTYTWSETHHRCLQLASSLSSSG
Sbjct: 39  MEDLKPRPPNSAPLTPIGFLERAATIYGNFPSIIYGDTTYTWSETHHRCLQLASSLSSSG 98

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLL HSESKLVFVDQA
Sbjct: 99  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQA 158

Query: 121 YCALISDALALFPPETDPPSLVLIDDAAAAEESLSSSISIRTDFVDSYEEMIQKGDSKFQ 180
           YCALISDALALFPPET+PPSLVLIDD AAAEESLSSS+SIR DF DSYEEMIQKGD KFQ
Sbjct: 159 YCALISDALALFPPETNPPSLVLIDDDAAAEESLSSSVSIRIDFFDSYEEMIQKGDYKFQ 218

Query: 181 WIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMFH 240
           WIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTL MFH
Sbjct: 219 WIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSMFH 278

Query: 241 INGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNQPLD 300
           INGWSFSWGIAAVGGTN+CIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDN+ LD
Sbjct: 279 INGWSFSWGIAAVGGTNICIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSLD 338

Query: 301 RPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQARL 360
           RPVKILTGG+PPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPA  QARL
Sbjct: 339 RPVKILTGGSPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQARL 398

Query: 361 KARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMTDK 420
           KARQGVRTAAVAAVDVVD +TGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKS+TD+
Sbjct: 399 KARQGVRTAAVAAVDVVDSKTGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSITDE 458

Query: 421 GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARADE 480
           GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LYTNP+VNEAAVVAR DE
Sbjct: 459 GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPDE 518

Query: 481 FWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKYV 540
           FWGETPCAFVSLREGLTRKPTEEEII+YCRGKLPRFMVPKTVVF+AELPKTSTGKIKKYV
Sbjct: 519 FWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKKYV 578

Query: 541 LKEIAMSIGPKPSRL 555
           LKE+AMSIGPKPSRL
Sbjct: 579 LKEMAMSIGPKPSRL 593

BLAST of Csor.00g290520 vs. ExPASy TrEMBL
Match: A0A6J1JKT9 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111487797 PE=4 SV=1)

HSP 1 Score: 933 bits (2412), Expect = 0.0
Identity = 456/549 (83.06%), Postives = 495/549 (90.16%), Query Frame = 0

Query: 1   MEDLKPRTPNCAPLTPIGFLERAATIYRDSPSIIYGHTTYTWSETHHRCLQLASSLSSSG 60
           MEDLKPR  N +PLTP+GFLERAATIY DSPSIIYG TTYTWS+TH RCLQLASSLSS G
Sbjct: 70  MEDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHCRCLQLASSLSSMG 129

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVDQA 120
           IQRGH+VSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTIS LL+HSESKL+FVDQA
Sbjct: 130 IQRGHIVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLTHSESKLIFVDQA 189

Query: 121 YCALISDALALFPPETDPPSLVLIDDAAAAEESLSSSISIRTDFVDSYEEMIQKGDSKFQ 180
           +CALI DALALFPPET  P LVLI D AAAEES SS I I  DFVDSYEEMI KGDS F+
Sbjct: 190 FCALIHDALALFPPETKRPMLVLITDDAAAEESSSSCIMIHDDFVDSYEEMILKGDSDFR 249

Query: 181 WIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMFH 240
           WIRP+SEWDPIVLNYTSGTTSSPKGVV CHRG+FI   D LLEW V K SVYLWTLPMFH
Sbjct: 250 WIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVQKQSVYLWTLPMFH 309

Query: 241 INGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNQPLD 300
            NGWS +WG+AAVGGTN+CIRKFDAALI++LIRRHHVTH+C APVVLNMLTNS  N+P D
Sbjct: 310 GNGWSLTWGMAAVGGTNICIRKFDAALIYTLIRRHHVTHLCGAPVVLNMLTNSPTNRPFD 369

Query: 301 RPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQARL 360
            PVKILT GAPPP+ VL R ESLGFDVTHGYGLTETAG V+ CSWK EWN+LP  E+ARL
Sbjct: 370 WPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQLPTKERARL 429

Query: 361 KARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMTDK 420
           K+RQGVRT A+ AVDVVD +TGKSVKQDGVSIGEIV++GGSIM+GY KDR+AT KSMT++
Sbjct: 430 KSRQGVRTVAMTAVDVVDQKTGKSVKQDGVSIGEIVLKGGSIMLGYLKDRDATTKSMTEE 489

Query: 421 GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARADE 480
           GWF TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLY NPSVNEAAVVAR DE
Sbjct: 490 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNEAAVVARPDE 549

Query: 481 FWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKYV 540
           FWGETPCAFVS+REGLT+KPTEEEII++CRGKLPRFMVPKTVVFM ELPKTSTGKI+KY+
Sbjct: 550 FWGETPCAFVSIREGLTQKPTEEEIIEHCRGKLPRFMVPKTVVFMTELPKTSTGKIQKYL 609

Query: 541 LKEIAMSIG 549
           L+E+AMS+G
Sbjct: 610 LREMAMSMG 618

BLAST of Csor.00g290520 vs. ExPASy TrEMBL
Match: A0A6J1JUG4 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111487609 PE=4 SV=1)

HSP 1 Score: 931 bits (2405), Expect = 0.0
Identity = 455/550 (82.73%), Postives = 495/550 (90.00%), Query Frame = 0

Query: 1   MEDLKPRTPNCAPLTPIGFLERAATIYRDSPSIIYGHTTYTWSETHHRCLQLASSLSSSG 60
           ME LKPR  N +PLTPIGFLERAATIY DSPSIIYG+TTYTWS+TH RCLQLASSLSS G
Sbjct: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSSIG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPM GAILNTINTRLDARTIS LL HSESKL+FVD+A
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDRA 120

Query: 121 YCALISDALALFPPETDPPSLVLIDDAAAAEESLSSSISIRTDFVDSYEEMIQKGDSKFQ 180
           +C LI +AL LFPPE + P LVL+ D AAAEES SSS+ I  +FVDSYE MI+KGDS+FQ
Sbjct: 121 FCNLIHNALDLFPPEINRPMLVLVADDAAAEES-SSSVLIEGNFVDSYEGMIRKGDSEFQ 180

Query: 181 WIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMFH 240
           WIRP SEWDPIVLNYTSGTTSSPKGVVHCHRG FI   D LLEWGVPK SVYLWTLPMFH
Sbjct: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFH 240

Query: 241 INGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNQPLD 300
            NGWSFSWG+AAVGGTN+CIRKFDA L++SLIRRHHVTH+C APVVLNMLTNS D +PLD
Sbjct: 241 ANGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLD 300

Query: 301 RPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQARL 360
            PVKILT GAP PA +LFRIESLGFDVTHGYGLTET G V+CCSWK EW++LPA E+ARL
Sbjct: 301 WPVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARL 360

Query: 361 KARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMTDK 420
           K+RQGVRT A  AVDVVDLETG+SV +DGVS+GEIV+R GS+M+GY+KD EATAKSMT+ 
Sbjct: 361 KSRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTED 420

Query: 421 GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARADE 480
           GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLY NPSVNEAAVVAR  E
Sbjct: 421 GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPHE 480

Query: 481 FWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKYV 540
           FWGETPCAFVSLREGLT++PTEEEII+YCRGKLPRFM PKTVVFM ELPKTSTGKI+KY+
Sbjct: 481 FWGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKYL 540

Query: 541 LKEIAMSIGP 550
           LKE+AMS+GP
Sbjct: 541 LKEMAMSMGP 549

BLAST of Csor.00g290520 vs. ExPASy TrEMBL
Match: A0A6J1JT47 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111487610 PE=4 SV=1)

HSP 1 Score: 926 bits (2392), Expect = 0.0
Identity = 454/550 (82.55%), Postives = 492/550 (89.45%), Query Frame = 0

Query: 1   MEDLKPRTPNCAPLTPIGFLERAATIYRDSPSIIYGHTTYTWSETHHRCLQLASSLSSSG 60
           MEDLKPR  N +PLTPIGFLERAATIY DSPSIIYG+TTYTWS+TH RCLQLASSLSS G
Sbjct: 1   MEDLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSSIG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPM GAILNTINTRLDARTIS LL H ESKL+FVD A
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHGESKLIFVDHA 120

Query: 121 YCALISDALALFPPETDPPSLVLIDDAAAAEESLSSSISIRTDFVDSYEEMIQKGDSKFQ 180
           +C LI +AL LFPPE   P LVLI D AAAEES SSS+ I  +FVDSYE MI+KGDS+FQ
Sbjct: 121 FCNLIHNALDLFPPEIYRPMLVLIADDAAAEES-SSSVLIEGNFVDSYEGMIRKGDSEFQ 180

Query: 181 WIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMFH 240
           WIRP SEWDPIVLNYTSGTTSSPKGVVHCHRG FI   D LLEWGVPK SVYLWTLPMFH
Sbjct: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFH 240

Query: 241 INGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNQPLD 300
            NGWSFSWG+AAVGGTN+CIRKFDA L++SLIRRHHVTH+C APVVLNMLTNS D +PLD
Sbjct: 241 ANGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLD 300

Query: 301 RPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQARL 360
            PVKILT GAP PA +LFRIESLGFDVTHGYGLTET G V+CCSWK EW++LPA E+ARL
Sbjct: 301 WPVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARL 360

Query: 361 KARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMTDK 420
           K+RQGVRT A  AVDVVDLETG+SV +DGVS+GEIV+R GS+M+GY+KD EATAKSMT+ 
Sbjct: 361 KSRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTED 420

Query: 421 GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARADE 480
           GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLY NPSVNEAAVVAR  E
Sbjct: 421 GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPHE 480

Query: 481 FWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKYV 540
           FWGETPCAFVSLREGLT++ TEEEII+YCRGKLPRFM PKTVVFM ELPKTSTGKI+KY+
Sbjct: 481 FWGETPCAFVSLREGLTQQRTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKYL 540

Query: 541 LKEIAMSIGP 550
           L+E+AMS+GP
Sbjct: 541 LREMAMSMGP 549

BLAST of Csor.00g290520 vs. TAIR 10
Match: AT5G16370.1 (acyl activating enzyme 5 )

HSP 1 Score: 750.0 bits (1935), Expect = 1.4e-216
Identity = 365/550 (66.36%), Postives = 446/550 (81.09%), Query Frame = 0

Query: 1   MEDLKPRTPNCAPLTPIGFLERAATIYRDSPSIIYG-HTTYTWSETHHRCLQLASSLSSS 60
           ME +KP   N  PLTPIGFLERAAT+Y D  SI+YG +T YTW ET+ RCL++ASSLSS 
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVDQ 120
           GI R  VVSV+SPNTP MYEL FAVPM+GAILN INTRLDART+S LL H  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 AYCALISDALALFPPETDPPSLVLIDDAAAAEESLSSSISIRTDFVDSYEEMIQKGDSKF 180
               L  +A+++    TDPP LV I D    EE   + ++ RT F  +Y+++I +GD  F
Sbjct: 121 FSVDLAVEAISMM--TTDPPILVFIAD--KEEEGGDADVADRTKFSYTYDDLIHRGDLDF 180

Query: 181 QWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMF 240
           +WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRG+F+ + DSL++W VPKN VYLWTLP+F
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIF 240

Query: 241 HINGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNQPL 300
           H NGWS+ WGIAAVGGTNVC+RKFDA LI+ LIR H VTHMC APVVLNML+ + + QPL
Sbjct: 241 HANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPL 300

Query: 301 DRPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQAR 360
           +RPV ILT GAPPPAAVL R ES+GF ++HGYGLTETAG+ + C+WK +WNRLPA+++AR
Sbjct: 301 NRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRAR 360

Query: 361 LKARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMTD 420
           LKARQGVRT     +DVVD E+G+SV+++G ++GEIV+RG SIM+GY KD   T K++ +
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKALKN 420

Query: 421 KGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARAD 480
            GWF TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYTNP+VNE AVVAR D
Sbjct: 421 -GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKY 540
            FWGETPCAFVSL+ GLT++PTE E+I+YCR K+P++MVPKTV F+ ELPKTSTGK+ K+
Sbjct: 481 VFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKF 540

Query: 541 VLKEIAMSIG 550
           VL+EIA  +G
Sbjct: 541 VLREIAKKMG 545

BLAST of Csor.00g290520 vs. TAIR 10
Match: AT5G16340.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 738.8 bits (1906), Expect = 3.2e-213
Identity = 357/552 (64.67%), Postives = 446/552 (80.80%), Query Frame = 0

Query: 1   MEDLKPRTPNCAPLTPIGFLERAATIYRDSPSIIYG-HTTYTWSETHHRCLQLASSLSSS 60
           ME++KP   N  PLTPIGFLERAAT+Y D  SI+YG +T YTW ET+ RCL++ASSLSS 
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVDQ 120
           GI R  VVSV+SPNTP MYEL FAVPM+GAILN INTRLDART+S LL H ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 AYCALISDALALFPPETDPPSLVLIDDAAAAEESLSSSISIRTDFVDSYEEMIQKGDSKF 180
               L  +A+++    TDPP LV+I D    EE   + ++  + F  +Y+++I++GD  F
Sbjct: 121 FSVDLAVEAVSMM--TTDPPILVVIAD--KEEEGGVADVADLSKFSYTYDDLIERGDPGF 180

Query: 181 QWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMF 240
           +WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRG+F+ + DSL++W VPKN VYLWTLP+F
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIF 240

Query: 241 HINGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNQPL 300
           H NGW+  WGIAAVGGTNVC+RKFDA LI+ LIR H VTHMC APVVLNML+ + ++QPL
Sbjct: 241 HSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPL 300

Query: 301 DRPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQAR 360
           + PV ILT G+PPPA VL R ES+GF ++HGYGLTETAG+++ C+WK +WN LPA+++AR
Sbjct: 301 NHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRAR 360

Query: 361 LKARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMTD 420
           LKARQGVRT     +DVVD E+G SV+++G ++GEIV+RG S+M+GY KD   T K++ +
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKALKN 420

Query: 421 KGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARAD 480
            GWF TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYT P+VNE AVVAR D
Sbjct: 421 -GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKY 540
           EFWGETPCAFVSL+ G + KPTEEE+++YCR K+P++MVPKTV FM ELPK+STGK+ K+
Sbjct: 481 EFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKF 540

Query: 541 VLKEIAMSIGPK 552
           VL++IA  +G K
Sbjct: 541 VLRDIAKKMGDK 547

BLAST of Csor.00g290520 vs. TAIR 10
Match: AT1G75960.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 714.1 bits (1842), Expect = 8.4e-206
Identity = 355/550 (64.55%), Postives = 433/550 (78.73%), Query Frame = 0

Query: 1   MEDLKPRTPNCAPLTPIGFLERAATIYRDSPSIIYGHTT-YTWSETHHRCLQLASSLSSS 60
           MEDLKP   N  PLT +GFLERAAT+Y D  SI+YG++T YTW ET+HRCL +AS+LSS 
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVDQ 120
           GI R  VVSV+S NTP MYEL F+VPM+GAILN INTRLDART+S LL H ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 AYCALISDALALFPPETDPPSLVLIDDAAAAEESLSSSISIRTDFVDSYEEMIQKGDSKF 180
            Y  L  +A+ +     +PP LVLI  A   EE   + ++ R+ F   Y ++I +G+  F
Sbjct: 121 FYSDLAVEAITML---LNPPILVLI--ANEEEEEGGAEVTERSKFCYLYSDLITRGNPDF 180

Query: 181 QWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMF 240
           +WIRP SEWDPIV+NYTSGTTSSPKGVVHCHRG+F+   DSL +W VPK  VYLWTLP+F
Sbjct: 181 KWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIF 240

Query: 241 HINGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLD-NQP 300
           H NGW++ WGIAAVGGTNVC+RK  A  I+ LIR H VTHM  AP+VL +L+ S + +QP
Sbjct: 241 HANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQP 300

Query: 301 LDRPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQA 360
           L  PV  LT G+ PPA VL R ESLGF V+HGYGLTETAG+++ C+WK  WNRLPA++QA
Sbjct: 301 LKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQA 360

Query: 361 RLKARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMT 420
           +LK+RQGVRT   + +DVVD E+G+SV++DG ++GEIV+RG SIM+GY K+   T  S  
Sbjct: 361 QLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSFK 420

Query: 421 DKGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARA 480
           + GWF TGD+GV+H DGYLEIKDRSKDVIISGGEN+SSVEVE+VLYTNP+VNEAAVVAR 
Sbjct: 421 N-GWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARP 480

Query: 481 DEFWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKK 540
           DEFWGETPCAFVSL+ GLTRKPT++EII+YC+ K+PR+M PKTV F+ ELPKTSTGKI K
Sbjct: 481 DEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIK 540

Query: 541 YVLKEIAMSI 549
            +LKEIA ++
Sbjct: 541 SLLKEIAKNM 544

BLAST of Csor.00g290520 vs. TAIR 10
Match: AT1G77240.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 685.6 bits (1768), Expect = 3.2e-197
Identity = 327/550 (59.45%), Postives = 425/550 (77.27%), Query Frame = 0

Query: 1   MEDLKPRTPNCAPLTPIGFLERAATIYRDSPSIIYGHTTYTWSETHHRCLQLASSLSSS- 60
           ME L P   N  PLT +GFLERAA+++ DSPS+++  T +TWSETH RCL++AS+LSS+ 
Sbjct: 1   MELLLPHASNSCPLTVLGFLERAASVFGDSPSLLHTTTVHTWSETHSRCLRIASTLSSAS 60

Query: 61  -GIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVD 120
            GI RG VVSVI PN P +YEL FAVPM+GA+LN IN RLDA  +S LL HSESKLVFVD
Sbjct: 61  LGINRGQVVSVIGPNVPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVD 120

Query: 121 QAYCALISDALALFPPETDPPSLVLIDDAAAAEESLSSSISIRTDFVDSYEEMIQKGDSK 180
               +L+ +A++ F P+ + P LV+++D       + SS S   DF+D+YE  +++GD +
Sbjct: 121 HHSSSLVLEAVS-FLPKDERPRLVILNDG----NDMPSSSSADMDFLDTYEGFMERGDLR 180

Query: 181 FQWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPM 240
           F+W+RP SEW P+VLNYTSGTTSSPKGVVH HR VF++  +SLL+W +P   VYLWTLPM
Sbjct: 181 FKWVRPKSEWTPMVLNYTSGTTSSPKGVVHSHRSVFMSTINSLLDWSLPNRPVYLWTLPM 240

Query: 241 FHINGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNQP 300
           FH NGWS++W  AAVG  N+C+ + D   IF+LI ++ VTHMCAAP+VLNMLTN    +P
Sbjct: 241 FHANGWSYTWATAAVGARNICVTRVDVPTIFNLIDKYQVTHMCAAPMVLNMLTNHPAQKP 300

Query: 301 LDRPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQA 360
           L  PVK++T GAPPPA V+ + E+LGFDV+HGYG+TET G+V+ C+ K EW+RL   E+A
Sbjct: 301 LQSPVKVMTAGAPPPATVISKAEALGFDVSHGYGMTETGGLVVSCALKPEWDRLEPDERA 360

Query: 361 RLKARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMT 420
           + K+RQG+RTA  A VDV D  +GKSVK DG ++GEIV RGGS+M+GY+KD E TA SM 
Sbjct: 361 KQKSRQGIRTAVFAEVDVRDPISGKSVKHDGATVGEIVFRGGSVMLGYYKDPEGTAASMR 420

Query: 421 DKGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARA 480
           + GWF TGD+GVMHPDGYLE+KDRSKDV+I GGEN+SS E+E+VLYTNP++ EAAVVA+ 
Sbjct: 421 EDGWFYTGDIGVMHPDGYLEVKDRSKDVVICGGENISSTELEAVLYTNPAIKEAAVVAKP 480

Query: 481 DEFWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKK 540
           D+ WGETPCAFVSL+       TE EI ++C+ KLP++MVP+ VVF+ ELPKTSTGKI+K
Sbjct: 481 DKMWGETPCAFVSLKYH-DGSVTEREIREFCKTKLPKYMVPRNVVFLEELPKTSTGKIQK 540

Query: 541 YVLKEIAMSI 549
           ++L+++A S+
Sbjct: 541 FLLRQMAKSL 544

BLAST of Csor.00g290520 vs. TAIR 10
Match: AT1G21540.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 681.0 bits (1756), Expect = 7.9e-196
Identity = 333/556 (59.89%), Postives = 426/556 (76.62%), Query Frame = 0

Query: 1   MEDLKPRTPNCAPLTPIGFLERAATIYRDSPSIIY-GHTTYTWSETHHRCLQLASSLSSS 60
           ME L P   N  PLT +GFL+RAA++Y D PSI++  +T +TWSETH+RCL++AS+L+SS
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 61  --GIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFV 120
             GI RG VVSV+ PN P +YEL FAVPM+GAILN IN RLDA  +S LL HSESKLVFV
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 121 DQAYCALISDALALFPPETDPPSLVLIDDAAAAEESLSSSISIRTDFVDSYEEMIQKGDS 180
           D    +++ +A++ F  + + P LVL+DD    +E  S S S  +DF+D+Y+ ++++GDS
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDD---DQEDGSLSPSAASDFLDTYQGVMERGDS 180

Query: 181 KFQWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLP 240
           +F+WIRP +EW P++LNYTSGTTSSPKGVV  HR +F+    SLL+W  P   VYLWTLP
Sbjct: 181 RFKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWTLP 240

Query: 241 MFHINGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNQ 300
           MFH NGW ++WG AAVG TNVC R+ DA  I+ LI +HHVTHMCAAP+VLNMLTN    +
Sbjct: 241 MFHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPSRK 300

Query: 301 PLDRPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQ 360
           PL  PV+++T GAPPPAA++ R E+LGF+V HGYGLTET G V+ C+WK EW+ L   E+
Sbjct: 301 PLKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPLER 360

Query: 361 ARLKARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSM 420
           ARLK+RQGVRT   A VDV D  TGKSV+ DGVS+GEIV++GGS+M+GY+KD E TA  M
Sbjct: 361 ARLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAACM 420

Query: 421 TDKGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVAR 480
            + GWF +GDVGV+H DGYLE+KDRSKDVII GGEN+SS EVE+VLYTNP V EAAVVA+
Sbjct: 421 REDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVVAK 480

Query: 481 ADEFWGETPCAFVSLR-----EGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTS 540
            D+ WGETPCAFVSL+      GL    TE EI ++C+ +LP++MVP+ V+F  ELPKTS
Sbjct: 481 PDKMWGETPCAFVSLKYDSNGNGLV---TEREIREFCKTRLPKYMVPRKVIFQEELPKTS 540

Query: 541 TGKIKKYVLKEIAMSI 549
           TGKI+K++L+++A S+
Sbjct: 541 TGKIQKFLLRQMAKSL 549