Csor.00g290490 (gene) Silver-seed gourd (wild; sororia) v1

BLAST of Csor.00g290490 vs. ExPASy Swiss-Prot
Match: M4IQQ5 (2-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1)

HSP 1 Score: 826.2 bits (2133), Expect = 2.4e-238
Identity = 393/558 (70.43%), Postives = 464/558 (83.15%), Query Frame = 0

Query: 65  EDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHRRCLQLASSLSSIGI 124
           EDLKPRPA+SSPLTPLGFLERAAT+YGD  S++Y   +YTWS THRRCL LASS++S+GI
Sbjct: 2   EDLKPRPASSSPLTPLGFLERAATVYGDCTSVVYDAVSYTWSQTHRRCLCLASSIASLGI 61

Query: 125 QRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHSESKLIFVDQAF 184
           + GHVVSV++PN P MYELHFAVPMAGAILN +N RLDARTISILL HSESKLIFVD   
Sbjct: 62  ENGHVVSVLAPNVPQMYELHFAVPMAGAILNAVNLRLDARTISILLHHSESKLIFVDHLS 121

Query: 185 CALIHDALALFPPKTKRPMLVLITDDAAAEESSLSCIMIHDDFVDSYEEMIRKGDSDFRW 244
             LI +A+ALFP +   P LV + D++ +  SS     +  +F  SY+++I +GD DF+W
Sbjct: 122 RDLILEAIALFPKQAPVPRLVFMADESESGNSS----ELGKEFFCSYKDLIDRGDPDFKW 181

Query: 245 IRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVPKQSVYLWTLPMFHG 304
           + P SEWDP++LNYTSGTTSSPKGVV CHRGIFI+T+D L++W VPKQ VYLWTLPMFH 
Sbjct: 182 VMPKSEWDPMILNYTSGTTSSPKGVVHCHRGIFIMTVDSLIDWGVPKQPVYLWTLPMFHA 241

Query: 305 NGWSLTWGMAAVGGTNICIRKFDAALIYSLIRRHHVTHLCGAPVVLNMLTNSPTNRPFDW 364
           NGWS  WGMAAVGGTNIC+RKFD+ +IY +I+RH VTH+CGAPVVLNML+N+P + P   
Sbjct: 242 NGWSYPWGMAAVGGTNICLRKFDSEIIYDMIKRHGVTHMCGAPVVLNMLSNAPGSEPLKT 301

Query: 365 PVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQLPTTERARLK 424
            V+I+T GAPPPS VL RTESLGF V+HGYGLTETAG VV C+WK EWN LP TERARLK
Sbjct: 302 TVQIMTAGAPPPSAVLFRTESLGFAVSHGYGLTETAGLVVSCAWKKEWNHLPATERARLK 361

Query: 425 SRQGVRTVAMTAVDVVDQKTGESVKRDGISIGEIVLRGGSIMLGYLKDRDATTKSMTDEG 484
           SRQGV TV  T +DVVD  TG +VKRDG ++GE+VLRGGS+MLGYLKD + T KSMT +G
Sbjct: 362 SRQGVGTVMQTKIDVVDPVTGAAVKRDGSTLGEVVLRGGSVMLGYLKDPEGTAKSMTADG 421

Query: 485 WFFSGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNEAAVVARPDEF 544
           WF++GDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LY +P + EAAVVARPDEF
Sbjct: 422 WFYTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYSHPDILEAAVVARPDEF 481

Query: 545 WGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPKTSTGKIQKYLL 604
           WGETPCAFVSL++GL +KPTEKEI+E+CR KLPR+MVPKTVVF  ELPKTSTGK+QK++L
Sbjct: 482 WGETPCAFVSLKKGLTKKPTEKEIVEYCRSKLPRYMVPKTVVFKEELPKTSTGKVQKFIL 541

Query: 605 REMAMSMGHANHPQSKEK 623
           R+MA  MG A    S+ +
Sbjct: 542 RDMARGMGSATAGASRSR 555

BLAST of Csor.00g290490 vs. ExPASy Swiss-Prot
Match: M4ISH2 (Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1)

HSP 1 Score: 766.9 bits (1979), Expect = 1.7e-220
Identity = 363/557 (65.17%), Postives = 451/557 (80.97%), Query Frame = 0

Query: 65  EDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHRRCLQLASSLSS-IG 124
           E+LKPR ANS PLTPLGFLERAAT+YGD  S++Y D +YTWS TH RCL++AS + S +G
Sbjct: 2   EELKPRFANSCPLTPLGFLERAATVYGDCISVVYDDLSYTWSQTHTRCLRVASCIESCLG 61

Query: 125 IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHSESKLIFVDQA 184
           +++G VVSV++PN P MYEL+FAVPMAG +LN INTRL+A TIS++L HSESKL+FVDQ 
Sbjct: 62  VKKGQVVSVVAPNVPAMYELNFAVPMAGVVLNNINTRLNAVTISVMLRHSESKLVFVDQL 121

Query: 185 FCALIHDALALFPPKTKRPMLVLITDDAAAEESSLSCIMIHDDFVDSYEEMIRKG-DSDF 244
              L+ DA++LFP  T  P+LVLI D+   E  +   +   + FV  YE+++ KG D  F
Sbjct: 122 SVRLVLDAVSLFPKNTPTPLLVLIADNITGENLT---VEDREHFVCCYEDLVEKGDDKTF 181

Query: 245 RWIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVPKQSVYLWTLPMF 304
           +W+RP+SEWDPIVLNYTSGTTSSPKGVV  HR +F++TLD L++W VPKQ VYLWTLPMF
Sbjct: 182 KWVRPISEWDPIVLNYTSGTTSSPKGVVHSHRSVFVVTLDSLIDWGVPKQPVYLWTLPMF 241

Query: 305 HGNGWSLTWGMAAVGGTNICIRKFDAALIYSLIRRHHVTHLCGAPVVLNMLTNSPTNRPF 364
           H NGW  TWG+AAVGGTNIC+R+FD  +I++LIRRH VTH+C AP+VLNML+NSP   P 
Sbjct: 242 HANGWGYTWGIAAVGGTNICLRRFDGEIIFNLIRRHRVTHMCAAPIVLNMLSNSPNAEPL 301

Query: 365 DWPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQLPTTERAR 424
             PV ++T GAPPP+ VLLRTESLGF ++HGYG+TE  G VV C+WK EWN+LP TE+AR
Sbjct: 302 PNPVHVMTGGAPPPAAVLLRTESLGFVISHGYGMTEMLGVVVSCAWKREWNRLPATEQAR 361

Query: 425 LKSRQGVRTVAMTAVDVVDQKTGESVKRDGISIGEIVLRGGSIMLGYLKDRDATTKSMTD 484
           LKSRQGVRT AM  VDVVD  +G SVKRDG+++GEIVL+G SIMLGYLK+  AT K +  
Sbjct: 362 LKSRQGVRTAAMMEVDVVDPNSGVSVKRDGLTMGEIVLKGSSIMLGYLKNSAATAKCIRA 421

Query: 485 EGWFFSGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNEAAVVARPD 544
           +GWF++GD+ VMHPDGYLEIKDRSKDVIISGGEN+SSVEVES LY +P+V+EAAVVA PD
Sbjct: 422 DGWFYTGDMAVMHPDGYLEIKDRSKDVIISGGENVSSVEVESALYSHPAVDEAAVVACPD 481

Query: 545 EFWGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPKTSTGKIQKY 604
           E+WGETP AFV+L++G+  +PTEKEI+E+CREKL  FMVPK VVF  +LPKTSTGKIQK+
Sbjct: 482 EYWGETPFAFVTLKKGMRVRPTEKEILEYCREKLAHFMVPKVVVFRDKLPKTSTGKIQKF 541

Query: 605 LLREMAMSMGHANHPQS 620
            L+E+  +MG+++   S
Sbjct: 542 TLKEIVKTMGYSSSSSS 555

BLAST of Csor.00g290490 vs. ExPASy Swiss-Prot
Match: Q9FFE6 (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE5 PE=1 SV=1)

HSP 1 Score: 743.8 bits (1919), Expect = 1.6e-213
Identity = 358/549 (65.21%), Postives = 444/549 (80.87%), Query Frame = 0

Query: 65  EDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYG-DTTYTWSDTHRRCLQLASSLSSIG 124
           E +KP  ANS PLTP+GFLERAAT+YGD  SI+YG +T YTW +T+ RCL++ASSLSSIG
Sbjct: 2   EQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSIG 61

Query: 125 IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHSESKLIFVDQA 184
           I R  VVSV+SPNTP MYEL FAVPM+GAILN INTRLDART+S+LL H  SKL+FVD  
Sbjct: 62  IGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDVF 121

Query: 185 FCALIHDALALFPPKTKRPMLVLITDDAAAEESSLSCIMIHDDFVDSYEEMIRKGDSDFR 244
              L  +A+++    T  P+LV I D    EE   + +     F  +Y+++I +GD DF+
Sbjct: 122 SVDLAVEAISMM--TTDPPILVFIAD--KEEEGGDADVADRTKFSYTYDDLIHRGDLDFK 181

Query: 245 WIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVPKQSVYLWTLPMFH 304
           WIRP SEWDP+VLNYTSGTTS+PKGVV CHRGIF++++D L++W VPK  VYLWTLP+FH
Sbjct: 182 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIFH 241

Query: 305 GNGWSLTWGMAAVGGTNICIRKFDAALIYSLIRRHHVTHLCGAPVVLNMLTNSPTNRPFD 364
            NGWS  WG+AAVGGTN+C+RKFDA LIY LIR H VTH+CGAPVVLNML+ +   +P +
Sbjct: 242 ANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPLN 301

Query: 365 WPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQLPTTERARL 424
            PV ILT GAPPP+ VLLR ES+GF ++HGYGLTETAG  V C+WK +WN+LP ++RARL
Sbjct: 302 RPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRARL 361

Query: 425 KSRQGVRTVAMTAVDVVDQKTGESVKRDGISIGEIVLRGGSIMLGYLKDRDATTKSMTDE 484
           K+RQGVRTV  T +DVVD ++G SV+R+G ++GEIV+RG SIMLGYLKD   T K++ + 
Sbjct: 362 KARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKALKN- 421

Query: 485 GWFFSGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNEAAVVARPDE 544
           GWF++GDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLY NP+VNE AVVARPD 
Sbjct: 422 GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPDV 481

Query: 545 FWGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPKTSTGKIQKYL 604
           FWGETPCAFVSL+ GL Q+PTE E+IE+CR+K+P++MVPKTV F+ ELPKTSTGK+ K++
Sbjct: 482 FWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKFV 541

Query: 605 LREMAMSMG 613
           LRE+A  MG
Sbjct: 542 LREIAKKMG 545

BLAST of Csor.00g290490 vs. ExPASy Swiss-Prot
Match: Q9FFE9 (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 SV=1)

HSP 1 Score: 740.7 bits (1911), Expect = 1.3e-212
Identity = 352/549 (64.12%), Postives = 448/549 (81.60%), Query Frame = 0

Query: 65  EDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYG-DTTYTWSDTHRRCLQLASSLSSIG 124
           E++KP  ANS PLTP+GFLERAAT+YGD  SI+YG +T YTW +T+ RCL++ASSLSSIG
Sbjct: 2   EEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSIG 61

Query: 125 IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHSESKLIFVDQA 184
           I R  VVSV+SPNTP MYEL FAVPM+GAILN INTRLDART+S+LL H ESKL+FVD  
Sbjct: 62  IGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDVF 121

Query: 185 FCALIHDALALFPPKTKRPMLVLITDDAAAEESSLSCIMIHDDFVDSYEEMIRKGDSDFR 244
              L  +A+++    T  P+LV+I D    EE  ++ +     F  +Y+++I +GD  F+
Sbjct: 122 SVDLAVEAVSMM--TTDPPILVVIAD--KEEEGGVADVADLSKFSYTYDDLIERGDPGFK 181

Query: 245 WIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVPKQSVYLWTLPMFH 304
           WIRP SEWDP+VLNYTSGTTS+PKGVV CHRGIF++++D L++W VPK  VYLWTLP+FH
Sbjct: 182 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIFH 241

Query: 305 GNGWSLTWGMAAVGGTNICIRKFDAALIYSLIRRHHVTHLCGAPVVLNMLTNSPTNRPFD 364
            NGW+  WG+AAVGGTN+C+RKFDA LIY LIR H VTH+CGAPVVLNML+ +  ++P +
Sbjct: 242 SNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPLN 301

Query: 365 WPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQLPTTERARL 424
            PV ILT G+PPP+ VLLR ES+GF ++HGYGLTETAG +V C+WK +WN LP ++RARL
Sbjct: 302 HPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRARL 361

Query: 425 KSRQGVRTVAMTAVDVVDQKTGESVKRDGISIGEIVLRGGSIMLGYLKDRDATTKSMTDE 484
           K+RQGVRTV  T +DVVD ++G SV+R+G ++GEIV+RG S+MLGYLKD   T K++ + 
Sbjct: 362 KARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKALKN- 421

Query: 485 GWFFSGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNEAAVVARPDE 544
           GWF++GDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLY  P+VNE AVVARPDE
Sbjct: 422 GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPDE 481

Query: 545 FWGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPKTSTGKIQKYL 604
           FWGETPCAFVSL+ G + KPTE+E++E+CR+K+P++MVPKTV FM ELPK+STGK+ K++
Sbjct: 482 FWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKFV 541

Query: 605 LREMAMSMG 613
           LR++A  MG
Sbjct: 542 LRDIAKKMG 545

BLAST of Csor.00g290490 vs. ExPASy Swiss-Prot
Match: Q9LQS1 (Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 SV=1)

HSP 1 Score: 713.4 bits (1840), Expect = 2.3e-204
Identity = 354/549 (64.48%), Postives = 434/549 (79.05%), Query Frame = 0

Query: 65  EDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTT-YTWSDTHRRCLQLASSLSSIG 124
           EDLKP  ANS PLT LGFLERAAT+YGD  SI+YG++T YTW +T+ RCL +AS+LSSIG
Sbjct: 2   EDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSIG 61

Query: 125 IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHSESKLIFVDQA 184
           I R  VVSV+S NTP MYEL F+VPM+GAILN INTRLDART+S+LL H ESKL+FVD  
Sbjct: 62  IGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDFF 121

Query: 185 FCALIHDALALFPPKTKRPMLVLITDDAAAEESSLSCIMIHDDFVDSYEEMIRKGDSDFR 244
           +  L  +A+ +       P+LVLI ++   EE   + +     F   Y ++I +G+ DF+
Sbjct: 122 YSDLAVEAITML---LNPPILVLIANE--EEEEGGAEVTERSKFCYLYSDLITRGNPDFK 181

Query: 245 WIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVPKQSVYLWTLPMFH 304
           WIRP SEWDPIV+NYTSGTTSSPKGVV CHRGIF++TLD L +W VPK  VYLWTLP+FH
Sbjct: 182 WIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIFH 241

Query: 305 GNGWSLTWGMAAVGGTNICIRKFDAALIYSLIRRHHVTHLCGAPVVLNMLTNS-PTNRPF 364
            NGW+  WG+AAVGGTN+C+RK  A  IY LIR H VTH+ GAP+VL +L+ S  +++P 
Sbjct: 242 ANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQPL 301

Query: 365 DWPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQLPTTERAR 424
             PV  LT G+ PP+ VLLR ESLGF V+HGYGLTETAG +V C+WK  WN+LP +++A+
Sbjct: 302 KSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQAQ 361

Query: 425 LKSRQGVRTVAMTAVDVVDQKTGESVKRDGISIGEIVLRGGSIMLGYLKDRDATTKSMTD 484
           LKSRQGVRTV  + +DVVD ++G SV+RDG ++GEIVLRG SIMLGYLK+   T  S  +
Sbjct: 362 LKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSFKN 421

Query: 485 EGWFFSGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNEAAVVARPD 544
            GWFF+GD+GV+H DGYLEIKDRSKDVIISGGEN+SSVEVE+VLY NP+VNEAAVVARPD
Sbjct: 422 -GWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARPD 481

Query: 545 EFWGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPKTSTGKIQKY 604
           EFWGETPCAFVSL+ GL +KPT+KEIIE+C+ K+PR+M PKTV F+ ELPKTSTGKI K 
Sbjct: 482 EFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIKS 541

Query: 605 LLREMAMSM 612
           LL+E+A +M
Sbjct: 542 LLKEIAKNM 544

BLAST of Csor.00g290490 vs. NCBI nr
Match: KAG6602001.1 (putative acyl-activating enzyme 5, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1265 bits (3273), Expect = 0.0
Identity = 623/623 (100.00%), Postives = 623/623 (100.00%), Query Frame = 0

Query: 1   MCSTWFSWRPVKLTFKGLTDSALMCNDYNSNFRRSTLVPTHEAKRSISMKTSKRYIISSQ 60
           MCSTWFSWRPVKLTFKGLTDSALMCNDYNSNFRRSTLVPTHEAKRSISMKTSKRYIISSQ
Sbjct: 1   MCSTWFSWRPVKLTFKGLTDSALMCNDYNSNFRRSTLVPTHEAKRSISMKTSKRYIISSQ 60

Query: 61  SISTEDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHRRCLQLASSLS 120
           SISTEDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHRRCLQLASSLS
Sbjct: 61  SISTEDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHRRCLQLASSLS 120

Query: 121 SIGIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHSESKLIFV 180
           SIGIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHSESKLIFV
Sbjct: 121 SIGIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHSESKLIFV 180

Query: 181 DQAFCALIHDALALFPPKTKRPMLVLITDDAAAEESSLSCIMIHDDFVDSYEEMIRKGDS 240
           DQAFCALIHDALALFPPKTKRPMLVLITDDAAAEESSLSCIMIHDDFVDSYEEMIRKGDS
Sbjct: 181 DQAFCALIHDALALFPPKTKRPMLVLITDDAAAEESSLSCIMIHDDFVDSYEEMIRKGDS 240

Query: 241 DFRWIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVPKQSVYLWTLP 300
           DFRWIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVPKQSVYLWTLP
Sbjct: 241 DFRWIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVPKQSVYLWTLP 300

Query: 301 MFHGNGWSLTWGMAAVGGTNICIRKFDAALIYSLIRRHHVTHLCGAPVVLNMLTNSPTNR 360
           MFHGNGWSLTWGMAAVGGTNICIRKFDAALIYSLIRRHHVTHLCGAPVVLNMLTNSPTNR
Sbjct: 301 MFHGNGWSLTWGMAAVGGTNICIRKFDAALIYSLIRRHHVTHLCGAPVVLNMLTNSPTNR 360

Query: 361 PFDWPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQLPTTER 420
           PFDWPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQLPTTER
Sbjct: 361 PFDWPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQLPTTER 420

Query: 421 ARLKSRQGVRTVAMTAVDVVDQKTGESVKRDGISIGEIVLRGGSIMLGYLKDRDATTKSM 480
           ARLKSRQGVRTVAMTAVDVVDQKTGESVKRDGISIGEIVLRGGSIMLGYLKDRDATTKSM
Sbjct: 421 ARLKSRQGVRTVAMTAVDVVDQKTGESVKRDGISIGEIVLRGGSIMLGYLKDRDATTKSM 480

Query: 481 TDEGWFFSGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNEAAVVAR 540
           TDEGWFFSGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNEAAVVAR
Sbjct: 481 TDEGWFFSGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNEAAVVAR 540

Query: 541 PDEFWGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPKTSTGKIQ 600
           PDEFWGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPKTSTGKIQ
Sbjct: 541 PDEFWGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPKTSTGKIQ 600

Query: 601 KYLLREMAMSMGHANHPQSKEKK 623
           KYLLREMAMSMGHANHPQSKEKK
Sbjct: 601 KYLLREMAMSMGHANHPQSKEKK 623

BLAST of Csor.00g290490 vs. NCBI nr
Match: XP_023548760.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1228 bits (3178), Expect = 0.0
Identity = 606/623 (97.27%), Postives = 614/623 (98.56%), Query Frame = 0

Query: 1   MCSTWFSWRPVKLTFKGLTDSALMCNDYNSNFRRSTLVPTHEAKRSISMKTSKRYIISSQ 60
           MCSTWF WRPVKLTFKGLTDSALMCNDYNSNFR STLVPTHEAKRSISMKTSKRYI SSQ
Sbjct: 1   MCSTWFPWRPVKLTFKGLTDSALMCNDYNSNFRPSTLVPTHEAKRSISMKTSKRYI-SSQ 60

Query: 61  SISTEDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHRRCLQLASSLS 120
           SISTEDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHRRCLQLASSLS
Sbjct: 61  SISTEDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHRRCLQLASSLS 120

Query: 121 SIGIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHSESKLIFV 180
           SIGIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHSESKLIFV
Sbjct: 121 SIGIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHSESKLIFV 180

Query: 181 DQAFCALIHDALALFPPKTKRPMLVLITDDAAAEESSLSCIMIHDDFVDSYEEMIRKGDS 240
           DQAFCALIHDALALFPP+TKRPMLVLITDDAAAEESS SCIMIHD FVDSYEEMIRKGDS
Sbjct: 181 DQAFCALIHDALALFPPETKRPMLVLITDDAAAEESSSSCIMIHDYFVDSYEEMIRKGDS 240

Query: 241 DFRWIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVPKQSVYLWTLP 300
           DFRWIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVPKQSVYLWTLP
Sbjct: 241 DFRWIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVPKQSVYLWTLP 300

Query: 301 MFHGNGWSLTWGMAAVGGTNICIRKFDAALIYSLIRRHHVTHLCGAPVVLNMLTNSPTNR 360
           MFHGNGWSLTWGMAAVGGTNICIRKFDAALIY+LIRRHHVTHLCGAPVVLNMLTNSPTNR
Sbjct: 301 MFHGNGWSLTWGMAAVGGTNICIRKFDAALIYTLIRRHHVTHLCGAPVVLNMLTNSPTNR 360

Query: 361 PFDWPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQLPTTER 420
           PFDWPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQLPTTER
Sbjct: 361 PFDWPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQLPTTER 420

Query: 421 ARLKSRQGVRTVAMTAVDVVDQKTGESVKRDGISIGEIVLRGGSIMLGYLKDRDATTKSM 480
           ARLKSRQGVRTVAMTAVDVVDQKTGESVKRDG+SIGEIVLRGGSIMLGYLKDRDATTKSM
Sbjct: 421 ARLKSRQGVRTVAMTAVDVVDQKTGESVKRDGVSIGEIVLRGGSIMLGYLKDRDATTKSM 480

Query: 481 TDEGWFFSGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNEAAVVAR 540
           T+EGWFFSGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNEAAVVAR
Sbjct: 481 TEEGWFFSGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNEAAVVAR 540

Query: 541 PDEFWGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPKTSTGKIQ 600
           PDEFWGETPCAFVSLREGLAQKPTEKEIIEHCR KLPRFMVPK VVFMTELPKTSTGKIQ
Sbjct: 541 PDEFWGETPCAFVSLREGLAQKPTEKEIIEHCRGKLPRFMVPKMVVFMTELPKTSTGKIQ 600

Query: 601 KYLLREMAMSMGHANHPQSKEKK 623
           KY+LREMA+SMG A HP+S+EKK
Sbjct: 601 KYMLREMALSMGQAKHPRSQEKK 622

BLAST of Csor.00g290490 vs. NCBI nr
Match: XP_022922116.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata])

HSP 1 Score: 1204 bits (3115), Expect = 0.0
Identity = 594/630 (94.29%), Postives = 607/630 (96.35%), Query Frame = 0

Query: 1   MCSTWFSWRPVKLTFKGLTDSALMCNDYNSNFRRSTLVPTHEAKRS-------ISMKTSK 60
           MCSTWF WRPVKLTFK LTDSAL+CNDYNSNFRRSTLVP HEAKRS       IS K SK
Sbjct: 1   MCSTWFRWRPVKLTFKDLTDSALVCNDYNSNFRRSTLVPIHEAKRSTYLIFTNISKKASK 60

Query: 61  RYIISSQSISTEDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHRRCL 120
            ++ISS+SIST+DLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHRRCL
Sbjct: 61  HFVISSKSISTQDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHRRCL 120

Query: 121 QLASSLSSIGIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHS 180
           QLASSLSSIGIQRGH+VSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHS
Sbjct: 121 QLASSLSSIGIQRGHIVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHS 180

Query: 181 ESKLIFVDQAFCALIHDALALFPPKTKRPMLVLITDDAAAEESSLSCIMIHDDFVDSYEE 240
           ESKLIFVDQAFCALI DALALFPP+TKRPMLVLI DDAAAEESS SCIMI +DFVDSYEE
Sbjct: 181 ESKLIFVDQAFCALIQDALALFPPETKRPMLVLIADDAAAEESSSSCIMIDNDFVDSYEE 240

Query: 241 MIRKGDSDFRWIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVPKQS 300
           MIRKGDSDFRWIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEV KQS
Sbjct: 241 MIRKGDSDFRWIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVTKQS 300

Query: 301 VYLWTLPMFHGNGWSLTWGMAAVGGTNICIRKFDAALIYSLIRRHHVTHLCGAPVVLNML 360
           VYLWTLPMFHGNGWSLTWGMAAVGGTNIC+RKFDAALIY+LIRRHHVTHLCGAPVVLNML
Sbjct: 301 VYLWTLPMFHGNGWSLTWGMAAVGGTNICVRKFDAALIYTLIRRHHVTHLCGAPVVLNML 360

Query: 361 TNSPTNRPFDWPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWN 420
           TNSPTNRPFDWPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWN
Sbjct: 361 TNSPTNRPFDWPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWN 420

Query: 421 QLPTTERARLKSRQGVRTVAMTAVDVVDQKTGESVKRDGISIGEIVLRGGSIMLGYLKDR 480
           QL TTERARLKSRQGVRTVAMTAVDVVDQKTGESVKRDG+SIGEIVLRGGSIMLGYLKDR
Sbjct: 421 QLSTTERARLKSRQGVRTVAMTAVDVVDQKTGESVKRDGVSIGEIVLRGGSIMLGYLKDR 480

Query: 481 DATTKSMTDEGWFFSGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVN 540
           DATTKSMTDEGWFFSGDVGVMHPDGYLEIKDR KDVIISGGENLSSVEVESVLYMNPSVN
Sbjct: 481 DATTKSMTDEGWFFSGDVGVMHPDGYLEIKDRLKDVIISGGENLSSVEVESVLYMNPSVN 540

Query: 541 EAAVVARPDEFWGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPK 600
           EAAVVARPDEFWGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPK
Sbjct: 541 EAAVVARPDEFWGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPK 600

Query: 601 TSTGKIQKYLLREMAMSMGHANHPQSKEKK 623
           TSTGKIQKYLLREM MSMGHA HP+ +EKK
Sbjct: 601 TSTGKIQKYLLREMGMSMGHAKHPRWEEKK 630

BLAST of Csor.00g290490 vs. NCBI nr
Match: XP_022991102.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima])

HSP 1 Score: 1187 bits (3072), Expect = 0.0
Identity = 583/629 (92.69%), Postives = 603/629 (95.87%), Query Frame = 0

Query: 1   MCSTWFSWRPVKLTFKGLTDSALMCNDYNSNFRRSTLVPTHEAK------RSISMKTSKR 60
           MCSTWF WRP+KL FKGL DSAL+CNDYNSNFRRS LVPTHEAK       ++S K SK 
Sbjct: 1   MCSTWFPWRPIKLIFKGLPDSALVCNDYNSNFRRSMLVPTHEAKPTSLMFTNLSKKASKH 60

Query: 61  YIISSQSISTEDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHRRCLQ 120
           Y ISS+SIS EDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTH RCLQ
Sbjct: 61  YTISSKSISMEDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHCRCLQ 120

Query: 121 LASSLSSIGIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHSE 180
           LASSLSS+GIQRGH+VSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILL+HSE
Sbjct: 121 LASSLSSMGIQRGHIVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLTHSE 180

Query: 181 SKLIFVDQAFCALIHDALALFPPKTKRPMLVLITDDAAAEESSLSCIMIHDDFVDSYEEM 240
           SKLIFVDQAFCALIHDALALFPP+TKRPMLVLITDDAAAEESS SCIMIHDDFVDSYEEM
Sbjct: 181 SKLIFVDQAFCALIHDALALFPPETKRPMLVLITDDAAAEESSSSCIMIHDDFVDSYEEM 240

Query: 241 IRKGDSDFRWIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVPKQSV 300
           I KGDSDFRWIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEV KQSV
Sbjct: 241 ILKGDSDFRWIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVQKQSV 300

Query: 301 YLWTLPMFHGNGWSLTWGMAAVGGTNICIRKFDAALIYSLIRRHHVTHLCGAPVVLNMLT 360
           YLWTLPMFHGNGWSLTWGMAAVGGTNICIRKFDAALIY+LIRRHHVTHLCGAPVVLNMLT
Sbjct: 301 YLWTLPMFHGNGWSLTWGMAAVGGTNICIRKFDAALIYTLIRRHHVTHLCGAPVVLNMLT 360

Query: 361 NSPTNRPFDWPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQ 420
           NSPTNRPFDWPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQ
Sbjct: 361 NSPTNRPFDWPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQ 420

Query: 421 LPTTERARLKSRQGVRTVAMTAVDVVDQKTGESVKRDGISIGEIVLRGGSIMLGYLKDRD 480
           LPT ERARLKSRQGVRTVAMTAVDVVDQKTG+SVK+DG+SIGEIVL+GGSIMLGYLKDRD
Sbjct: 421 LPTKERARLKSRQGVRTVAMTAVDVVDQKTGKSVKQDGVSIGEIVLKGGSIMLGYLKDRD 480

Query: 481 ATTKSMTDEGWFFSGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNE 540
           ATTKSMT+EGWFF+GDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNE
Sbjct: 481 ATTKSMTEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNE 540

Query: 541 AAVVARPDEFWGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPKT 600
           AAVVARPDEFWGETPCAFVS+REGL QKPTE+EIIEHCR KLPRFMVPKTVVFMTELPKT
Sbjct: 541 AAVVARPDEFWGETPCAFVSIREGLTQKPTEEEIIEHCRGKLPRFMVPKTVVFMTELPKT 600

Query: 601 STGKIQKYLLREMAMSMGHANHPQSKEKK 623
           STGKIQKYLLREMAMSMG A HP+S+ KK
Sbjct: 601 STGKIQKYLLREMAMSMGRAKHPRSEGKK 629

BLAST of Csor.00g290490 vs. NCBI nr
Match: KAG6601999.1 (putative acyl-activating enzyme 5, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 970 bits (2508), Expect = 0.0
Identity = 475/548 (86.68%), Postives = 500/548 (91.24%), Query Frame = 0

Query: 65  EDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHRRCLQLASSLSSIGI 124
           EDLKPRPANSSPLTP+GFLERAA IYGDSPSIIYG TTYTWSDTHRRCLQLASSLSSIGI
Sbjct: 2   EDLKPRPANSSPLTPIGFLERAAIIYGDSPSIIYGHTTYTWSDTHRRCLQLASSLSSIGI 61

Query: 125 QRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHSESKLIFVDQAF 184
           QRGHVVSVISPNTPPMYELHFAVPM GAILNTINTRLDARTISILL HSESKLIFVDQAF
Sbjct: 62  QRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQAF 121

Query: 185 CALIHDALALFPPKTKRPMLVLITDDAAAEESSLSCIMIHDDFVDSYEEMIRKGDSDFRW 244
           C LIH+AL LFPP+  RPMLVLI DDA AEESS S ++I  DF+ SYEEMIRKGDSDFRW
Sbjct: 122 CNLIHNALDLFPPEINRPMLVLIADDAVAEESS-SSVLIDGDFLGSYEEMIRKGDSDFRW 181

Query: 245 IRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVPKQSVYLWTLPMFHG 304
           IRP SEWDPI LNYTSGTTSSPKGVV CHRG FIIT+D+LLEW VPKQSVYLWTLPMFH 
Sbjct: 182 IRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFHA 241

Query: 305 NGWSLTWGMAAVGGTNICIRKFDAALIYSLIRRHHVTHLCGAPVVLNMLTNSPTNRPFDW 364
           NGWS +WGMAAVGGTNICIRKFDAAL+YSLIRRHHVTHLCGAPVVLNMLTNSP  RP DW
Sbjct: 242 NGWSFSWGMAAVGGTNICIRKFDAALVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 301

Query: 365 PVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQLPTTERARLK 424
           PVKILT GAP P+ +L R ESLGFDVTHGYGLTET G VV CSWKAEWNQLP  ERARLK
Sbjct: 302 PVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAEERARLK 361

Query: 425 SRQGVRTVAMTAVDVVDQKTGESVKRDGISIGEIVLRGGSIMLGYLKDRDATTKSMTDEG 484
           SRQGVRTVA  AVDVVD +TGESV RDG+S+GEIVLR GS+MLGY KD +AT KSMT+EG
Sbjct: 362 SRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTEEG 421

Query: 485 WFFSGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNEAAVVARPDEF 544
           WFF+GDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LY NPSVNEAAVVARP EF
Sbjct: 422 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPHEF 481

Query: 545 WGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPKTSTGKIQKYLL 604
           WGETPCAFVSLREGL Q+PTE+EIIE+C+ KLPRFM PKTVVFMTELPKTSTGKIQKYLL
Sbjct: 482 WGETPCAFVSLREGLTQQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 541

Query: 605 REMAMSMG 612
           +EMAMSMG
Sbjct: 542 KEMAMSMG 548

BLAST of Csor.00g290490 vs. ExPASy TrEMBL
Match: A0A6J1E391 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111430182 PE=4 SV=1)

HSP 1 Score: 1204 bits (3115), Expect = 0.0
Identity = 594/630 (94.29%), Postives = 607/630 (96.35%), Query Frame = 0

Query: 1   MCSTWFSWRPVKLTFKGLTDSALMCNDYNSNFRRSTLVPTHEAKRS-------ISMKTSK 60
           MCSTWF WRPVKLTFK LTDSAL+CNDYNSNFRRSTLVP HEAKRS       IS K SK
Sbjct: 1   MCSTWFRWRPVKLTFKDLTDSALVCNDYNSNFRRSTLVPIHEAKRSTYLIFTNISKKASK 60

Query: 61  RYIISSQSISTEDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHRRCL 120
            ++ISS+SIST+DLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHRRCL
Sbjct: 61  HFVISSKSISTQDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHRRCL 120

Query: 121 QLASSLSSIGIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHS 180
           QLASSLSSIGIQRGH+VSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHS
Sbjct: 121 QLASSLSSIGIQRGHIVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHS 180

Query: 181 ESKLIFVDQAFCALIHDALALFPPKTKRPMLVLITDDAAAEESSLSCIMIHDDFVDSYEE 240
           ESKLIFVDQAFCALI DALALFPP+TKRPMLVLI DDAAAEESS SCIMI +DFVDSYEE
Sbjct: 181 ESKLIFVDQAFCALIQDALALFPPETKRPMLVLIADDAAAEESSSSCIMIDNDFVDSYEE 240

Query: 241 MIRKGDSDFRWIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVPKQS 300
           MIRKGDSDFRWIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEV KQS
Sbjct: 241 MIRKGDSDFRWIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVTKQS 300

Query: 301 VYLWTLPMFHGNGWSLTWGMAAVGGTNICIRKFDAALIYSLIRRHHVTHLCGAPVVLNML 360
           VYLWTLPMFHGNGWSLTWGMAAVGGTNIC+RKFDAALIY+LIRRHHVTHLCGAPVVLNML
Sbjct: 301 VYLWTLPMFHGNGWSLTWGMAAVGGTNICVRKFDAALIYTLIRRHHVTHLCGAPVVLNML 360

Query: 361 TNSPTNRPFDWPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWN 420
           TNSPTNRPFDWPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWN
Sbjct: 361 TNSPTNRPFDWPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWN 420

Query: 421 QLPTTERARLKSRQGVRTVAMTAVDVVDQKTGESVKRDGISIGEIVLRGGSIMLGYLKDR 480
           QL TTERARLKSRQGVRTVAMTAVDVVDQKTGESVKRDG+SIGEIVLRGGSIMLGYLKDR
Sbjct: 421 QLSTTERARLKSRQGVRTVAMTAVDVVDQKTGESVKRDGVSIGEIVLRGGSIMLGYLKDR 480

Query: 481 DATTKSMTDEGWFFSGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVN 540
           DATTKSMTDEGWFFSGDVGVMHPDGYLEIKDR KDVIISGGENLSSVEVESVLYMNPSVN
Sbjct: 481 DATTKSMTDEGWFFSGDVGVMHPDGYLEIKDRLKDVIISGGENLSSVEVESVLYMNPSVN 540

Query: 541 EAAVVARPDEFWGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPK 600
           EAAVVARPDEFWGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPK
Sbjct: 541 EAAVVARPDEFWGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPK 600

Query: 601 TSTGKIQKYLLREMAMSMGHANHPQSKEKK 623
           TSTGKIQKYLLREM MSMGHA HP+ +EKK
Sbjct: 601 TSTGKIQKYLLREMGMSMGHAKHPRWEEKK 630

BLAST of Csor.00g290490 vs. ExPASy TrEMBL
Match: A0A6J1JKT9 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111487797 PE=4 SV=1)

HSP 1 Score: 1187 bits (3072), Expect = 0.0
Identity = 583/629 (92.69%), Postives = 603/629 (95.87%), Query Frame = 0

Query: 1   MCSTWFSWRPVKLTFKGLTDSALMCNDYNSNFRRSTLVPTHEAK------RSISMKTSKR 60
           MCSTWF WRP+KL FKGL DSAL+CNDYNSNFRRS LVPTHEAK       ++S K SK 
Sbjct: 1   MCSTWFPWRPIKLIFKGLPDSALVCNDYNSNFRRSMLVPTHEAKPTSLMFTNLSKKASKH 60

Query: 61  YIISSQSISTEDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHRRCLQ 120
           Y ISS+SIS EDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTH RCLQ
Sbjct: 61  YTISSKSISMEDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHCRCLQ 120

Query: 121 LASSLSSIGIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHSE 180
           LASSLSS+GIQRGH+VSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILL+HSE
Sbjct: 121 LASSLSSMGIQRGHIVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLTHSE 180

Query: 181 SKLIFVDQAFCALIHDALALFPPKTKRPMLVLITDDAAAEESSLSCIMIHDDFVDSYEEM 240
           SKLIFVDQAFCALIHDALALFPP+TKRPMLVLITDDAAAEESS SCIMIHDDFVDSYEEM
Sbjct: 181 SKLIFVDQAFCALIHDALALFPPETKRPMLVLITDDAAAEESSSSCIMIHDDFVDSYEEM 240

Query: 241 IRKGDSDFRWIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVPKQSV 300
           I KGDSDFRWIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEV KQSV
Sbjct: 241 ILKGDSDFRWIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVQKQSV 300

Query: 301 YLWTLPMFHGNGWSLTWGMAAVGGTNICIRKFDAALIYSLIRRHHVTHLCGAPVVLNMLT 360
           YLWTLPMFHGNGWSLTWGMAAVGGTNICIRKFDAALIY+LIRRHHVTHLCGAPVVLNMLT
Sbjct: 301 YLWTLPMFHGNGWSLTWGMAAVGGTNICIRKFDAALIYTLIRRHHVTHLCGAPVVLNMLT 360

Query: 361 NSPTNRPFDWPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQ 420
           NSPTNRPFDWPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQ
Sbjct: 361 NSPTNRPFDWPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQ 420

Query: 421 LPTTERARLKSRQGVRTVAMTAVDVVDQKTGESVKRDGISIGEIVLRGGSIMLGYLKDRD 480
           LPT ERARLKSRQGVRTVAMTAVDVVDQKTG+SVK+DG+SIGEIVL+GGSIMLGYLKDRD
Sbjct: 421 LPTKERARLKSRQGVRTVAMTAVDVVDQKTGKSVKQDGVSIGEIVLKGGSIMLGYLKDRD 480

Query: 481 ATTKSMTDEGWFFSGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNE 540
           ATTKSMT+EGWFF+GDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNE
Sbjct: 481 ATTKSMTEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNE 540

Query: 541 AAVVARPDEFWGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPKT 600
           AAVVARPDEFWGETPCAFVS+REGL QKPTE+EIIEHCR KLPRFMVPKTVVFMTELPKT
Sbjct: 541 AAVVARPDEFWGETPCAFVSIREGLTQKPTEEEIIEHCRGKLPRFMVPKTVVFMTELPKT 600

Query: 601 STGKIQKYLLREMAMSMGHANHPQSKEKK 623
           STGKIQKYLLREMAMSMG A HP+S+ KK
Sbjct: 601 STGKIQKYLLREMAMSMGRAKHPRSEGKK 629

BLAST of Csor.00g290490 vs. ExPASy TrEMBL
Match: A0A6J1E5Q3 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111430192 PE=4 SV=1)

HSP 1 Score: 966 bits (2496), Expect = 0.0
Identity = 473/547 (86.47%), Postives = 501/547 (91.59%), Query Frame = 0

Query: 65  EDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHRRCLQLASSLSSIGI 124
           E+LKPRPANSSPLTPLGFLERAATIYGDS SIIYG TTYTWS THRRCLQLASSLSSIGI
Sbjct: 2   EELKPRPANSSPLTPLGFLERAATIYGDSASIIYGHTTYTWSHTHRRCLQLASSLSSIGI 61

Query: 125 QRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHSESKLIFVDQAF 184
           QRGHVVSVISPNTPPMYELHFAVPM GAILNTINTRLDARTISILL HSESKLIFVDQAF
Sbjct: 62  QRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQAF 121

Query: 185 CALIHDALALFPPKTKRPMLVLITDDAAAEESSLSCIMIHDDFVDSYEEMIRKGDSDFRW 244
           C LIH++L LFPP+  RPMLVLI DDA AEESS S ++I  +FV SYEEMIRKGDS+F+W
Sbjct: 122 CNLIHNSLDLFPPEINRPMLVLIADDAVAEESS-SSVLIEGNFVGSYEEMIRKGDSEFQW 181

Query: 245 IRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVPKQSVYLWTLPMFHG 304
           IRP SEWDPIVLNYTSGTTSSPKGVV CHRG FI+TLD+LLEW VPKQSVYLWTLPMFH 
Sbjct: 182 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 241

Query: 305 NGWSLTWGMAAVGGTNICIRKFDAALIYSLIRRHHVTHLCGAPVVLNMLTNSPTNRPFDW 364
           NGWS +WG+AAVGGTNICIRKFD+AL+YSLIRRHHVTHLCGAPVVLNMLTNSP  RP DW
Sbjct: 242 NGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRPLDW 301

Query: 365 PVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQLPTTERARLK 424
           PVKILT GAP P+ +LLR ESLGFDVTHGYGLTET G VV CSWKAEWNQLP  ERARLK
Sbjct: 302 PVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERARLK 361

Query: 425 SRQGVRTVAMTAVDVVDQKTGESVKRDGISIGEIVLRGGSIMLGYLKDRDATTKSMTDEG 484
           SRQGVRTVA  AV+VVD +TG+SV RDG+SIGEIVLR GS+MLGY KD +AT KSMT+EG
Sbjct: 362 SRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTEEG 421

Query: 485 WFFSGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNEAAVVARPDEF 544
           WFF+GDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LY NPSVNEAAVVARPDEF
Sbjct: 422 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPDEF 481

Query: 545 WGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPKTSTGKIQKYLL 604
           WGETPCAFVSLREGL QKPTE+EIIE C+ KLPRFMVPKTVVFMTELPKTSTGKIQKYLL
Sbjct: 482 WGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKYLL 541

Query: 605 REMAMSM 611
           REMAMSM
Sbjct: 542 REMAMSM 547

BLAST of Csor.00g290490 vs. ExPASy TrEMBL
Match: A0A6J1JUG4 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111487609 PE=4 SV=1)

HSP 1 Score: 962 bits (2487), Expect = 0.0
Identity = 470/548 (85.77%), Postives = 500/548 (91.24%), Query Frame = 0

Query: 65  EDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHRRCLQLASSLSSIGI 124
           E LKPRPANSSPLTP+GFLERAATIY DSPSIIYG+TTYTWSDTHRRCLQLASSLSSIGI
Sbjct: 2   EHLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSSIGI 61

Query: 125 QRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHSESKLIFVDQAF 184
           QRGHVVSVISPNTPPMYELHFAVPM GAILNTINTRLDARTISILL HSESKLIFVD+AF
Sbjct: 62  QRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDRAF 121

Query: 185 CALIHDALALFPPKTKRPMLVLITDDAAAEESSLSCIMIHDDFVDSYEEMIRKGDSDFRW 244
           C LIH+AL LFPP+  RPMLVL+ DDAAAEESS S ++I  +FVDSYE MIRKGDS+F+W
Sbjct: 122 CNLIHNALDLFPPEINRPMLVLVADDAAAEESS-SSVLIEGNFVDSYEGMIRKGDSEFQW 181

Query: 245 IRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVPKQSVYLWTLPMFHG 304
           IRP SEWDPIVLNYTSGTTSSPKGVV CHRG FI+TLD+LLEW VPKQSVYLWTLPMFH 
Sbjct: 182 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 241

Query: 305 NGWSLTWGMAAVGGTNICIRKFDAALIYSLIRRHHVTHLCGAPVVLNMLTNSPTNRPFDW 364
           NGWS +WGMAAVGGTNICIRKFDA L+YSLIRRHHVTHLCGAPVVLNMLTNSP  RP DW
Sbjct: 242 NGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 301

Query: 365 PVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQLPTTERARLK 424
           PVKILT GAP P+ +L R ESLGFDVTHGYGLTET G VV CSWKAEW+QLP  ERARLK
Sbjct: 302 PVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 361

Query: 425 SRQGVRTVAMTAVDVVDQKTGESVKRDGISIGEIVLRGGSIMLGYLKDRDATTKSMTDEG 484
           SRQGVRTVA  AVDVVD +TGESV RDG+S+GEIVLR GS+MLGY KD +AT KSMT++G
Sbjct: 362 SRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTEDG 421

Query: 485 WFFSGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNEAAVVARPDEF 544
           WF +GDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLY NPSVNEAAVVARP EF
Sbjct: 422 WFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPHEF 481

Query: 545 WGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPKTSTGKIQKYLL 604
           WGETPCAFVSLREGL Q+PTE+EIIE+CR KLPRFM PKTVVFMTELPKTSTGKIQKYLL
Sbjct: 482 WGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 541

Query: 605 REMAMSMG 612
           +EMAMSMG
Sbjct: 542 KEMAMSMG 548

BLAST of Csor.00g290490 vs. ExPASy TrEMBL
Match: A0A6J1JT47 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111487610 PE=4 SV=1)

HSP 1 Score: 960 bits (2482), Expect = 0.0
Identity = 471/548 (85.95%), Postives = 498/548 (90.88%), Query Frame = 0

Query: 65  EDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHRRCLQLASSLSSIGI 124
           EDLKPRPANSSPLTP+GFLERAATIY DSPSIIYG+TTYTWSDTHRRCLQLASSLSSIGI
Sbjct: 2   EDLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSSIGI 61

Query: 125 QRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHSESKLIFVDQAF 184
           QRGHVVSVISPNTPPMYELHFAVPM GAILNTINTRLDARTISILL H ESKLIFVD AF
Sbjct: 62  QRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHGESKLIFVDHAF 121

Query: 185 CALIHDALALFPPKTKRPMLVLITDDAAAEESSLSCIMIHDDFVDSYEEMIRKGDSDFRW 244
           C LIH+AL LFPP+  RPMLVLI DDAAAEESS S ++I  +FVDSYE MIRKGDS+F+W
Sbjct: 122 CNLIHNALDLFPPEIYRPMLVLIADDAAAEESS-SSVLIEGNFVDSYEGMIRKGDSEFQW 181

Query: 245 IRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVPKQSVYLWTLPMFHG 304
           IRP SEWDPIVLNYTSGTTSSPKGVV CHRG FI+TLD+LLEW VPKQSVYLWTLPMFH 
Sbjct: 182 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 241

Query: 305 NGWSLTWGMAAVGGTNICIRKFDAALIYSLIRRHHVTHLCGAPVVLNMLTNSPTNRPFDW 364
           NGWS +WGMAAVGGTNICIRKFDA L+YSLIRRHHVTHLCGAPVVLNMLTNSP  RP DW
Sbjct: 242 NGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 301

Query: 365 PVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQLPTTERARLK 424
           PVKILT GAP P+ +L R ESLGFDVTHGYGLTET G VV CSWKAEW+QLP  ERARLK
Sbjct: 302 PVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 361

Query: 425 SRQGVRTVAMTAVDVVDQKTGESVKRDGISIGEIVLRGGSIMLGYLKDRDATTKSMTDEG 484
           SRQGVRTVA  AVDVVD +TGESV RDG+S+GEIVLR GS+MLGY KD +AT KSMT++G
Sbjct: 362 SRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTEDG 421

Query: 485 WFFSGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNEAAVVARPDEF 544
           WF +GDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLY NPSVNEAAVVARP EF
Sbjct: 422 WFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPHEF 481

Query: 545 WGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPKTSTGKIQKYLL 604
           WGETPCAFVSLREGL Q+ TE+EIIE+CR KLPRFM PKTVVFMTELPKTSTGKIQKYLL
Sbjct: 482 WGETPCAFVSLREGLTQQRTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 541

Query: 605 REMAMSMG 612
           REMAMSMG
Sbjct: 542 REMAMSMG 548

BLAST of Csor.00g290490 vs. TAIR 10
Match: AT5G16370.1 (acyl activating enzyme 5 )

HSP 1 Score: 743.8 bits (1919), Expect = 1.1e-214
Identity = 358/549 (65.21%), Postives = 444/549 (80.87%), Query Frame = 0

Query: 65  EDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYG-DTTYTWSDTHRRCLQLASSLSSIG 124
           E +KP  ANS PLTP+GFLERAAT+YGD  SI+YG +T YTW +T+ RCL++ASSLSSIG
Sbjct: 2   EQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSIG 61

Query: 125 IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHSESKLIFVDQA 184
           I R  VVSV+SPNTP MYEL FAVPM+GAILN INTRLDART+S+LL H  SKL+FVD  
Sbjct: 62  IGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDVF 121

Query: 185 FCALIHDALALFPPKTKRPMLVLITDDAAAEESSLSCIMIHDDFVDSYEEMIRKGDSDFR 244
              L  +A+++    T  P+LV I D    EE   + +     F  +Y+++I +GD DF+
Sbjct: 122 SVDLAVEAISMM--TTDPPILVFIAD--KEEEGGDADVADRTKFSYTYDDLIHRGDLDFK 181

Query: 245 WIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVPKQSVYLWTLPMFH 304
           WIRP SEWDP+VLNYTSGTTS+PKGVV CHRGIF++++D L++W VPK  VYLWTLP+FH
Sbjct: 182 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIFH 241

Query: 305 GNGWSLTWGMAAVGGTNICIRKFDAALIYSLIRRHHVTHLCGAPVVLNMLTNSPTNRPFD 364
            NGWS  WG+AAVGGTN+C+RKFDA LIY LIR H VTH+CGAPVVLNML+ +   +P +
Sbjct: 242 ANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPLN 301

Query: 365 WPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQLPTTERARL 424
            PV ILT GAPPP+ VLLR ES+GF ++HGYGLTETAG  V C+WK +WN+LP ++RARL
Sbjct: 302 RPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRARL 361

Query: 425 KSRQGVRTVAMTAVDVVDQKTGESVKRDGISIGEIVLRGGSIMLGYLKDRDATTKSMTDE 484
           K+RQGVRTV  T +DVVD ++G SV+R+G ++GEIV+RG SIMLGYLKD   T K++ + 
Sbjct: 362 KARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKALKN- 421

Query: 485 GWFFSGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNEAAVVARPDE 544
           GWF++GDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLY NP+VNE AVVARPD 
Sbjct: 422 GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPDV 481

Query: 545 FWGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPKTSTGKIQKYL 604
           FWGETPCAFVSL+ GL Q+PTE E+IE+CR+K+P++MVPKTV F+ ELPKTSTGK+ K++
Sbjct: 482 FWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKFV 541

Query: 605 LREMAMSMG 613
           LRE+A  MG
Sbjct: 542 LREIAKKMG 545

BLAST of Csor.00g290490 vs. TAIR 10
Match: AT5G16340.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 740.7 bits (1911), Expect = 9.4e-214
Identity = 352/549 (64.12%), Postives = 448/549 (81.60%), Query Frame = 0

Query: 65  EDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYG-DTTYTWSDTHRRCLQLASSLSSIG 124
           E++KP  ANS PLTP+GFLERAAT+YGD  SI+YG +T YTW +T+ RCL++ASSLSSIG
Sbjct: 2   EEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSIG 61

Query: 125 IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHSESKLIFVDQA 184
           I R  VVSV+SPNTP MYEL FAVPM+GAILN INTRLDART+S+LL H ESKL+FVD  
Sbjct: 62  IGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDVF 121

Query: 185 FCALIHDALALFPPKTKRPMLVLITDDAAAEESSLSCIMIHDDFVDSYEEMIRKGDSDFR 244
              L  +A+++    T  P+LV+I D    EE  ++ +     F  +Y+++I +GD  F+
Sbjct: 122 SVDLAVEAVSMM--TTDPPILVVIAD--KEEEGGVADVADLSKFSYTYDDLIERGDPGFK 181

Query: 245 WIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVPKQSVYLWTLPMFH 304
           WIRP SEWDP+VLNYTSGTTS+PKGVV CHRGIF++++D L++W VPK  VYLWTLP+FH
Sbjct: 182 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIFH 241

Query: 305 GNGWSLTWGMAAVGGTNICIRKFDAALIYSLIRRHHVTHLCGAPVVLNMLTNSPTNRPFD 364
            NGW+  WG+AAVGGTN+C+RKFDA LIY LIR H VTH+CGAPVVLNML+ +  ++P +
Sbjct: 242 SNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPLN 301

Query: 365 WPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQLPTTERARL 424
            PV ILT G+PPP+ VLLR ES+GF ++HGYGLTETAG +V C+WK +WN LP ++RARL
Sbjct: 302 HPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRARL 361

Query: 425 KSRQGVRTVAMTAVDVVDQKTGESVKRDGISIGEIVLRGGSIMLGYLKDRDATTKSMTDE 484
           K+RQGVRTV  T +DVVD ++G SV+R+G ++GEIV+RG S+MLGYLKD   T K++ + 
Sbjct: 362 KARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKALKN- 421

Query: 485 GWFFSGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNEAAVVARPDE 544
           GWF++GDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLY  P+VNE AVVARPDE
Sbjct: 422 GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPDE 481

Query: 545 FWGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPKTSTGKIQKYL 604
           FWGETPCAFVSL+ G + KPTE+E++E+CR+K+P++MVPKTV FM ELPK+STGK+ K++
Sbjct: 482 FWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKFV 541

Query: 605 LREMAMSMG 613
           LR++A  MG
Sbjct: 542 LRDIAKKMG 545

BLAST of Csor.00g290490 vs. TAIR 10
Match: AT1G75960.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 713.4 bits (1840), Expect = 1.6e-205
Identity = 354/549 (64.48%), Postives = 434/549 (79.05%), Query Frame = 0

Query: 65  EDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTT-YTWSDTHRRCLQLASSLSSIG 124
           EDLKP  ANS PLT LGFLERAAT+YGD  SI+YG++T YTW +T+ RCL +AS+LSSIG
Sbjct: 2   EDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSIG 61

Query: 125 IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHSESKLIFVDQA 184
           I R  VVSV+S NTP MYEL F+VPM+GAILN INTRLDART+S+LL H ESKL+FVD  
Sbjct: 62  IGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDFF 121

Query: 185 FCALIHDALALFPPKTKRPMLVLITDDAAAEESSLSCIMIHDDFVDSYEEMIRKGDSDFR 244
           +  L  +A+ +       P+LVLI ++   EE   + +     F   Y ++I +G+ DF+
Sbjct: 122 YSDLAVEAITML---LNPPILVLIANE--EEEEGGAEVTERSKFCYLYSDLITRGNPDFK 181

Query: 245 WIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVPKQSVYLWTLPMFH 304
           WIRP SEWDPIV+NYTSGTTSSPKGVV CHRGIF++TLD L +W VPK  VYLWTLP+FH
Sbjct: 182 WIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIFH 241

Query: 305 GNGWSLTWGMAAVGGTNICIRKFDAALIYSLIRRHHVTHLCGAPVVLNMLTNS-PTNRPF 364
            NGW+  WG+AAVGGTN+C+RK  A  IY LIR H VTH+ GAP+VL +L+ S  +++P 
Sbjct: 242 ANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQPL 301

Query: 365 DWPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQLPTTERAR 424
             PV  LT G+ PP+ VLLR ESLGF V+HGYGLTETAG +V C+WK  WN+LP +++A+
Sbjct: 302 KSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQAQ 361

Query: 425 LKSRQGVRTVAMTAVDVVDQKTGESVKRDGISIGEIVLRGGSIMLGYLKDRDATTKSMTD 484
           LKSRQGVRTV  + +DVVD ++G SV+RDG ++GEIVLRG SIMLGYLK+   T  S  +
Sbjct: 362 LKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSFKN 421

Query: 485 EGWFFSGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNEAAVVARPD 544
            GWFF+GD+GV+H DGYLEIKDRSKDVIISGGEN+SSVEVE+VLY NP+VNEAAVVARPD
Sbjct: 422 -GWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARPD 481

Query: 545 EFWGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPKTSTGKIQKY 604
           EFWGETPCAFVSL+ GL +KPT+KEIIE+C+ K+PR+M PKTV F+ ELPKTSTGKI K 
Sbjct: 482 EFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIKS 541

Query: 605 LLREMAMSM 612
           LL+E+A +M
Sbjct: 542 LLKEIAKNM 544

BLAST of Csor.00g290490 vs. TAIR 10
Match: AT1G21530.2 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 707.6 bits (1825), Expect = 8.9e-204
Identity = 341/554 (61.55%), Postives = 433/554 (78.16%), Query Frame = 0

Query: 65  EDLKPRPANSSPLTPLGFLERAATIYGDSPSIIY-GDTTYTWSDTHRRCLQLASSL--SS 124
           E L P P+NS+PLT LGFL+RAA++YGD PSI++  +T +TWS+TH RCL++AS+L  SS
Sbjct: 2   ELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTANTVHTWSETHNRCLRIASALTSSS 61

Query: 125 IGIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHSESKLIFVD 184
           IGI++G VVSV+ PN P +YEL FAVPM+GAILN IN RLDA  +S+LL HSES+L+FVD
Sbjct: 62  IGIKQGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESRLVFVD 121

Query: 185 QAFCALIHDALALFPPKTKRPMLVLITDDAAAEESSLSCIMIHDDFVDSYEEMIRKGDSD 244
               +L+ +A++LF  + ++P LVL+ DD   + SS S      DF+D+YEE++ +G+S 
Sbjct: 122 HRSISLVLEAVSLF-TQHEKPHLVLLDDDQENDSSSAS------DFLDTYEEIMERGNSR 181

Query: 245 FRWIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVPKQSVYLWTLPM 304
           F+WIRP +EW P+VLNYTSGTTSSPKGVV  HR IF++T+  LL+W VP + VYLWTLPM
Sbjct: 182 FKWIRPQTEWQPMVLNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWSVPNRPVYLWTLPM 241

Query: 305 FHGNGWSLTWGMAAVGGTNICIRKFDAALIYSLIRRHHVTHLCGAPVVLNMLTNSPTNRP 364
           FH NGW  TWG AAVG TNIC R+ DA  IY+LI +H+VTH+C AP+VLNML N P + P
Sbjct: 242 FHANGWGYTWGTAAVGATNICTRRVDAPTIYNLIDKHNVTHMCAAPMVLNMLINYPLSTP 301

Query: 365 FDWPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQLPTTERA 424
              PV+++T GAPPP+ ++ R ESLGF+V+H YGLTET+GPVV C+WK +W+ L   ERA
Sbjct: 302 LKNPVQVMTSGAPPPATIISRAESLGFNVSHSYGLTETSGPVVSCAWKPKWDHLDPLERA 361

Query: 425 RLKSRQGVRTVAMTAVDVVDQKTGESVKRDGISIGEIVLRGGSIMLGYLKDRDATTKSMT 484
           RLKSRQGVRT+  T VDV D+KTG+SVK DG+S+GEIV RG S+MLGY KD   T   M 
Sbjct: 362 RLKSRQGVRTLGFTEVDVRDRKTGKSVKHDGVSVGEIVFRGSSVMLGYYKDPQGTAACMR 421

Query: 485 DEGWFFSGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNEAAVVARP 544
           ++GWF+SGD+GV+H DGYLEIKDRSKDVII GGEN+SS E+E+VLY NP V EAAVVA+P
Sbjct: 422 EDGWFYSGDIGVIHKDGYLEIKDRSKDVIICGGENISSAEIETVLYTNPVVKEAAVVAKP 481

Query: 545 DEFWGETPCAFVSLR---EGLAQKP-TEKEIIEHCREKLPRFMVPKTVVFMTELPKTSTG 604
           D+ WGETPCAFVSL+    G    P TE+EI E C+ KLP++MVP+ V+F  ELPKTSTG
Sbjct: 482 DKMWGETPCAFVSLKCDNNGDGSVPVTEREIREFCKTKLPKYMVPRKVIFQEELPKTSTG 541

Query: 605 KIQKYLLREMAMSM 612
           KIQK+LLR+MA ++
Sbjct: 542 KIQKFLLRQMAKTL 548

BLAST of Csor.00g290490 vs. TAIR 10
Match: AT1G21540.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 706.1 bits (1821), Expect = 2.6e-203
Identity = 340/555 (61.26%), Postives = 432/555 (77.84%), Query Frame = 0

Query: 65  EDLKPRPANSSPLTPLGFLERAATIYGDSPSIIY-GDTTYTWSDTHRRCLQLASSL--SS 124
           E L P P+NS+PLT LGFL+RAA++YGD PSI++  +T +TWS+TH RCL++AS+L  SS
Sbjct: 2   ELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSSS 61

Query: 125 IGIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLSHSESKLIFVD 184
           +GI RG VVSV+ PN P +YEL FAVPM+GAILN IN RLDA  +S+LL HSESKL+FVD
Sbjct: 62  LGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFVD 121

Query: 185 QAFCALIHDALALFPPKTKRPMLVLITDDAAAEESSLSCIMIHDDFVDSYEEMIRKGDSD 244
               +++ +A++ F  + ++P LVL+ DD   E+ SLS      DF+D+Y+ ++ +GDS 
Sbjct: 122 PNSISVVLEAVS-FMRQNEKPHLVLLDDD--QEDGSLS-PSAASDFLDTYQGVMERGDSR 181

Query: 245 FRWIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVPKQSVYLWTLPM 304
           F+WIRP +EW P++LNYTSGTTSSPKGVV  HR IF++T+  LL+W  P + VYLWTLPM
Sbjct: 182 FKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWTLPM 241

Query: 305 FHGNGWSLTWGMAAVGGTNICIRKFDAALIYSLIRRHHVTHLCGAPVVLNMLTNSPTNRP 364
           FH NGW  TWG AAVG TN+C R+ DA  IY LI +HHVTH+C AP+VLNMLTN P+ +P
Sbjct: 242 FHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPSRKP 301

Query: 365 FDWPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQLPTTERA 424
              PV+++T GAPPP+ ++ R E+LGF+V HGYGLTET GPVV C+WKAEW+ L   ERA
Sbjct: 302 LKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPLERA 361

Query: 425 RLKSRQGVRTVAMTAVDVVDQKTGESVKRDGISIGEIVLRGGSIMLGYLKDRDATTKSMT 484
           RLKSRQGVRT+    VDV D +TG+SV+ DG+S+GEIVL+GGS+MLGY KD + T   M 
Sbjct: 362 RLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAACMR 421

Query: 485 DEGWFFSGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNEAAVVARP 544
           ++GWF+SGDVGV+H DGYLE+KDRSKDVII GGEN+SS EVE+VLY NP V EAAVVA+P
Sbjct: 422 EDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVVAKP 481

Query: 545 DEFWGETPCAFVSLR-----EGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTELPKTST 604
           D+ WGETPCAFVSL+      GL    TE+EI E C+ +LP++MVP+ V+F  ELPKTST
Sbjct: 482 DKMWGETPCAFVSLKYDSNGNGLV---TEREIREFCKTRLPKYMVPRKVIFQEELPKTST 541

Query: 605 GKIQKYLLREMAMSM 612
           GKIQK+LLR+MA S+
Sbjct: 542 GKIQKFLLRQMAKSL 549