Csor.00g289080 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g289080
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionwall-associated receptor kinase 3-like
LocationCsor_Chr04: 17902173 .. 17906341 (-)
RNA-Seq ExpressionCsor.00g289080
SyntenyCsor.00g289080
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAACACATCATGGCATTCATGGTGATGATAATGGAGATTGCCATATTATCAACAGCCTTGGCCATGGTAGCATCTCGAGCCTTACCCGGGTGTGACGAACAGTGTGGCGACGTGCAGATTCCATATCCATTCGGCATCAAAGAAGGGTGCTATCTCAATCAAAATTTCTCGATCACTTGCAACAAAACCGATCGCAATGGACCTCCAAAGGCGTATCTAATGCAGACCAATATTAGCGTTACCAATATATCCACTAACGGCGAGCTTCACGTGTTGCAGCCAGTAGTGCGAACTTGCTATGACCAAGTAAATGGTAGTTTTGTTCCCAAGGAATCCAATCTTTCTGTGCCGGCCATGTTCTCAATTTCTGGTACCAAAAACAAGTTCGTTACCATTGGGTGCAACACTGTTGGTATAATCGCTGGAATCTCCAAGGGAGTTTGTATGGAAGTGGGTGTATTTCGGTGTGTTTAAATAAGAGTAGTGTAGTTAATGGGCCGTGTTCTGGCAATGGGTGCTGTCAGTTGGAGTTTCCAAATGGGTTAACCAATTTGAGTTTGGCGGTGAGTGGGTTGTTGAATTTCACTGACGTAAGGACATTCAATCCCTGTGGGTATGCTTTTGTAATTGAAGCTGATAGGTTCCGGTTTTTGTCACGTTATATTGAGAATTTTGAAGAGGAAGTTGAGGTTGTGCTTGATTGGGGCATTAGAAATGAAACAAGATTTGAATGTGGATCAAACACCACAAGGAGTAGTATCCTTGATGGAACTGAATATCGTTGCCAATGCTTGGATGGTTACGAGGGGAATCCATATCTCCCTCAAGGATGCCAAGGTAAAGCCTTATTTATCTGAGTTATGTGAGATTTTACATTGGTTGGAGAGGAGGAAGAAGCATTTCTTATAAGGGTGTGGAAACCTCTCTCTAATAGACGCGTTTTAAAATTGTGAAGCTAATGGTGATATGTAATGGGCCAAAATGGACGATATCAGAGCTAGATACCGGACAGTGTGCCAGCAAAGACACTGGCCCCCAAGGGGGTGGATTGTGAGATCCCACATCGGTTGTAGAGGAAAATGAAGCATTTCTTGTAAGAGTGTGGATACCTCTCTCTAGCAAACATGTTTTAGAACCGTGAAGCTGATGACGAGGGCCAAAACTGGTAATATTTGTTAGCAGTGGGTTTGGGCTATTACAAAAGATATCAAAACTAAACATCGAACGGTGTTCTAGCGAGAACGCTGGCCCCTAAGGGTGTGTTTTGTGAGATCCCACATTGGTTGGAGAGAGAAACGAAACATTCCTTCTAAGAGTGGGGAAACCTCTCACTAGTAAACGCAATTTAAAACCGTGAGGCTGATGACGATACATAACAGGCCAAAACGGATGATATTTGCTAGCGGTGAGCTTGAGCTATTACAAATGGTTTCAGAGCTAGACACTGGACGGTGTGACAGCGGGGACGCAAGCCCCCAAGGAAGTGGATTGTGAGATCCCACGTCGATTTGAGAGGAGAACAAAACATTTCTTATAAGGGTGTGGAAACCTCTCCATAGTAGACAAGCCGTGAGGCTGACATCACTACGTAACAACGAGTCAAAACAGACAATATCTGCTAATGGTAGACTTGGACTTGGACGGTTACCGGTTAGGAGATTTTAAAATAAGTTTTATTAGGATTTGTAACCTAACCGCTGAATTGAACCCTTTTTAGATGTGGATGAATGCGAGTATGCATGGCTGAATGACTGTGAGTACAAGGACAAGTGTGCTAACACTAAAGGAAACTATACCTGTCATTGTCCTAAGAACTTCCATGGAGATGGAAGAGAAGGCGGAGAAGGTTGCACCAAAAATTCCACCTCTTCCATTCCTATCATCATTGGTGAGTTTTTATATGTTCTTAATGCATGTTTTTCACTATAATATACTAAACAACAACACAAATAAAATGCAGGGATTGGGGTAGGCCTCCCAGTTTTACTAATAGCCACCACAACCATCTACTTGGGTTACAAGAAATTGAAGTTCATCAAACAAAAACAGCTTTTCTTCCACAAAAATGGAGGCTTTGTGCTTCAACGACAGCTTTCTCAATGGAATTCACAGTGCGCTAGCTGTTGATCGGTGTTACAATATTCCAAGACAATTTCTTCGTCTGCAACAAGATCACGAATATAGTGATGTCGAAGTTCAATATGTTTTGTTCTACTGTGAAAGGCTGGGTTCTTTGTCATAGAAATAGTTGCTTTATTATCACAAAATATCTTTGTTGCCCCTTTTTGCTCTTGTTGAAGGTCAGCTAACATTCTTCTTAGCCAAATGGCTTGACACGCTGCTGAAGTTGCTGCAACATATTCTGCTTCTGATGTTGACAACGCCACTGTTGCTTGTTTCTTTGAGCTCCAAGTGATCGCTCCTGACCCTAAAGTGAAAACATTGGCTGAAATGCTTCGTCTATCATCTAATGATCCTGCCCAATCACTATCAGTGAATCCATATAGTTTGAAATTAGAGGATTTAGAATACCAAATTCCATACTCCATAGTTCCGGCAATGTAACGTAAGACTCTTTTTGCTGCTCCATAATGAACCTTTGAAGGATGTTGCATAAACTTAGAAATAATACCAACAGGAAATGCAATGTCGGGTCGTGTGTGAGTTAAGTAAATCAGTCCGCCAACCAAGCTCCTGAACCTTCTAGCATCTGTCATTTCTGCTCCATCATCATGTTGTAATTTCTCATTAATATTCATAGGTGTAGCTGCAGGCTTACAATTAAGCATACCAAATTTACTCATAATATCTTTGGCATATTTTCTTTGTGAAATAAAAATTCCATCTTCAACTTGATATACTTCAAGACCAAGAAAATAGTGCAATAGACCCATATCTGACATCTCAAATTCACTCATCATTTGAGACTTAAATTTATCCAAAAGAGAGTTAGAAGAGCTAGTGTAAATAATATCGTCAACATAGAGGCAAATAATGATGAAATCACTTTTACCTTCCTTCTTCACATATAAAGTAGGCTCATTCTCACTTCTCATGTATCCTTTCTTTTGAAAATACCCATCAATCTTGCTGTACCAAGCTCGAGGGGCCTGCCAAACGACATGGTTAGAATTTTCAGCCAAGAAGAGTTAGAGAAGGCCACCAACAACTACGCCCAGGACACCATTGCTGGCAAAGGTGGCTTCGGTACTGTTTACAAAGGTGTCTTGGACGATGGACTCACCATTGCAATCAAGAAATCAAAATTCATGGACGAATCCCAAACATCCCAGTTCATCAATGAAGTCATTGTTCTTTCCCAAATCAACCATCGAAACGTGGTCAAGCTCTTAGGGTGCTGTTTAGAGACCCAAGTCCCATTGTTGGTCTACGAGTTCGTCACCAACGGCACCCTCTTTGATCACATCCATGACACTACCAAACACGTCCCTCTTTCTTGGGAAGCTCGCTTGAGAATCGCTTCGGAAACAGCCGGTGTCATTTCGTATTTGCATTCTTCAGCTTCTATTCCCATTATCCACAGAGATATCAAGACGACCAACATACTTTTAGACGATAATTACACTGCAAAGGTGTCTGATTTTGGTGCTTCCAAGTTGGTTCCTATGGATCAAACTCAGCTAACCACGCTCGTGCAAGGGACTCTCGGGTACTTAGACCCTGAGTACTTATTGACGAGCGAGTTAACTGAGAAGAGTGATGTGTATAGCTTTGGAATTGTGCTTTTGGAGCTTATAACGGGGAAGAAAGCGGTGAGGTTTGATGGGCCGGAAGAAGATCGAAACCTAGCGATGTATGTCCTTTGTGCAATAAAAGAAGAGCGTTTGGAAGAAGTGGTGGAGAAGGGAATGGTAAGTGAGGCAAACATGGAGCAAATAAAGGAAGTGGCTAAGGTAGCAAGAGAGTGTGTAAGAATCAAAGGGGAGGAGAGGCCGAGCATGAAGGAGGTGGCTATGGAGTTGGAGGGGCTGCGTGCAACGAAGGGTGAGCATTCATGGGCCAATGTCAATTTATCCAGCGCAGAAGAGACGGTGTATTTGCTGGATGGAGCATCAGAGTCGAGCCAATTGGTTGGGAGTGGAAGTATGGGCACTGTGGGGAATAGCATAAAGCTGGAAATAACACTAATGGAGCATGGTAGATGA

mRNA sequence

ATGAAACACATCATGGCATTCATGGTGATGATAATGGAGATTGCCATATTATCAACAGCCTTGGCCATGGTAGCATCTCGAGCCTTACCCGGGTGTGACGAACAGTGTGGCGACGTGCAGATTCCATATCCATTCGGCATCAAAGAAGGGTGCTATCTCAATCAAAATTTCTCGATCACTTGCAACAAAACCGATCGCAATGGACCTCCAAAGGCGTATCTAATGCAGACCAATATTAGCGTTACCAATATATCCACTAACGGCGAGCTTCACGTGTTGCAGCCAGTAGTGCGAACTTGCTATGACCAAGTAAATGGTAGTTTTGTTCCCAAGGAATCCAATCTTTCTGTGCCGGCCATGTTCTCAATTTCTGGTACCAAAAACAAGTTCGTTACCATTGGGTGCAACACTGTTGGTATAATCGCTGGAATCTCCAAGGGAGTTTGTATGGAATTGGAGTTTCCAAATGGGTTAACCAATTTGAGTTTGGCGGTGAGTGGGTTGTTGAATTTCACTGACGTAAGGACATTCAATCCCTGTGGGTATGCTTTTGTAATTGAAGCTGATAGGTTCCGGTTTTTGTCACGTTATATTGAGAATTTTGAAGAGGAAGTTGAGGTTGTGCTTGATTGGGGCATTAGAAATGAAACAAGATTTGAATGTGGATCAAACACCACAAGGAGTAGTATCCTTGATGGAACTGAATATCGTTGCCAATGCTTGGATGGTTACGAGGGGAATCCATATCTCCCTCAAGGATGCCAAGATGTGGATGAATGCGAGTATGCATGGCTGAATGACTGTGAGTACAAGGACAAGTGTGCTAACACTAAAGGAAACTATACCTGTCATTGTCCTAAGAACTTCCATGGAGATGGAAGAGAAGGCGGAGAAGGTTGCACCAAAAATTCCACCTCTTCCATTCCTATCATCATTGAAGAGACGGTGTATTTGCTGGATGGAGCATCAGAGTCGAGCCAATTGGTTGGGAGTGGAAGTATGGGCACTGTGGGGAATAGCATAAAGCTGGAAATAACACTAATGGAGCATGGTAGATGA

Coding sequence (CDS)

ATGAAACACATCATGGCATTCATGGTGATGATAATGGAGATTGCCATATTATCAACAGCCTTGGCCATGGTAGCATCTCGAGCCTTACCCGGGTGTGACGAACAGTGTGGCGACGTGCAGATTCCATATCCATTCGGCATCAAAGAAGGGTGCTATCTCAATCAAAATTTCTCGATCACTTGCAACAAAACCGATCGCAATGGACCTCCAAAGGCGTATCTAATGCAGACCAATATTAGCGTTACCAATATATCCACTAACGGCGAGCTTCACGTGTTGCAGCCAGTAGTGCGAACTTGCTATGACCAAGTAAATGGTAGTTTTGTTCCCAAGGAATCCAATCTTTCTGTGCCGGCCATGTTCTCAATTTCTGGTACCAAAAACAAGTTCGTTACCATTGGGTGCAACACTGTTGGTATAATCGCTGGAATCTCCAAGGGAGTTTGTATGGAATTGGAGTTTCCAAATGGGTTAACCAATTTGAGTTTGGCGGTGAGTGGGTTGTTGAATTTCACTGACGTAAGGACATTCAATCCCTGTGGGTATGCTTTTGTAATTGAAGCTGATAGGTTCCGGTTTTTGTCACGTTATATTGAGAATTTTGAAGAGGAAGTTGAGGTTGTGCTTGATTGGGGCATTAGAAATGAAACAAGATTTGAATGTGGATCAAACACCACAAGGAGTAGTATCCTTGATGGAACTGAATATCGTTGCCAATGCTTGGATGGTTACGAGGGGAATCCATATCTCCCTCAAGGATGCCAAGATGTGGATGAATGCGAGTATGCATGGCTGAATGACTGTGAGTACAAGGACAAGTGTGCTAACACTAAAGGAAACTATACCTGTCATTGTCCTAAGAACTTCCATGGAGATGGAAGAGAAGGCGGAGAAGGTTGCACCAAAAATTCCACCTCTTCCATTCCTATCATCATTGAAGAGACGGTGTATTTGCTGGATGGAGCATCAGAGTCGAGCCAATTGGTTGGGAGTGGAAGTATGGGCACTGTGGGGAATAGCATAAAGCTGGAAATAACACTAATGGAGCATGGTAGATGA

Protein sequence

MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSITCNKTDRNGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYDQVNGSFVPKESNLSVPAMFSISGTKNKFVTIGCNTVGIIAGISKGVCMELEFPNGLTNLSLAVSGLLNFTDVRTFNPCGYAFVIEADRFRFLSRYIENFEEEVEVVLDWGIRNETRFECGSNTTRSSILDGTEYRCQCLDGYEGNPYLPQGCQDVDECEYAWLNDCEYKDKCANTKGNYTCHCPKNFHGDGREGGEGCTKNSTSSIPIIIEETVYLLDGASESSQLVGSGSMGTVGNSIKLEITLMEHGR
Homology
BLAST of Csor.00g289080 vs. ExPASy Swiss-Prot
Match: Q9LMP1 (Wall-associated receptor kinase 2 OS=Arabidopsis thaliana OX=3702 GN=WAK2 PE=1 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.1e-38
Identity = 96/295 (32.54%), Postives = 144/295 (48.81%), Query Frame = 0

Query: 32  CDEQCGDVQIPYPFGIKEGCYL--NQNFSITCNKTDRNGPPKAYLMQTNISVTNISTNGE 91
           C  +CG+V + YPFG   GCY   +++F++TCN+ ++       L   N+ V N+S +G+
Sbjct: 29  CQTRCGNVAVEYPFGTSPGCYYPGDESFNLTCNEQEK-------LFFGNMPVINMSLSGQ 88

Query: 92  LHVLQPVVRTCYDQVNGSFVPKESNLSVPAMFSISGTKNKFVTIGCNTVGII--AGISK- 151
           L V     R CYD   G      +  +    F++S   N+F  +GCN+   +  +G+ K 
Sbjct: 89  LRVRLVRSRVCYDS-QGKQTDYIAQRTTLGNFTLS-ELNRFTVVGCNSYAFLRTSGVEKY 148

Query: 152 --------------------GVCMELEFPNGLTNLSLAVSGLLNFTDVRTFNPCGYAFVI 211
                                 C ++  P G + + +      N   V  FNPC YAF++
Sbjct: 149 STGCISICDSATTKNGSCSGEGCCQIPVPRGYSFVRVKPHSFHNHPTVHLFNPCTYAFLV 208

Query: 212 EADRFRFLSRYIENFEEEV---EVVLDWGIRNET------RFECGSNTTRSSILDGTEYR 271
           E   F F +    N    V    VVLDW I ++T      R  CG N+T      GT Y 
Sbjct: 209 EDGMFDFHALEDLNNLRNVTTFPVVLDWSIGDKTCKQVEYRGVCGGNSTCFDSTGGTGYN 268

Query: 272 CQCLDGYEGNPYLPQGCQDVDECEYAWLNDCEYKDKCANTKGNYTCHCPKNFHGD 293
           C+CL+G+EGNPYLP GCQD++EC  +  ++C     C NTKG++ C+CP  +  D
Sbjct: 269 CKCLEGFEGNPYLPNGCQDINEC-ISSRHNCSEHSTCENTKGSFNCNCPSGYRKD 313

BLAST of Csor.00g289080 vs. ExPASy Swiss-Prot
Match: Q9LMN7 (Wall-associated receptor kinase 5 OS=Arabidopsis thaliana OX=3702 GN=WAK5 PE=2 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 8.7e-36
Identity = 104/320 (32.50%), Postives = 144/320 (45.00%), Query Frame = 0

Query: 9   VMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYL--NQNFSITCNKTDR 68
           + +M I        +V ++    C  +CGDV I YPFGI  GCY   + +F+ITC +   
Sbjct: 6   LFLMAIFFYLAYTQLVKAQPRDDCQTRCGDVPIDYPFGISTGCYYPGDDSFNITCEEDKP 65

Query: 69  NGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYD-QVNGSFVP---KESNLSVPAMFS 128
           N       + +NI V N + +G+L  L P    CYD Q N  F     +  NLS      
Sbjct: 66  N-------VLSNIEVLNFNHSGQLRGLIPRSTVCYDQQTNNDFESLWFRLDNLSF----- 125

Query: 129 ISGTKNKFVTIGCNTVGIIA--GISK----------------------GVC-MELEFPNG 188
                NKF  +GCN   +++  GI                        G C  E+  P  
Sbjct: 126 --SPNNKFTLVGCNAWALLSTFGIQNYSTGCMSLCDTPPPPNSKCNGVGCCRTEVSIPLD 185

Query: 189 LTNLSLAVSGLLNFTDVRTFNPCGYAFVIEADRFRFLSRYIENFEE-----EVEVVLDWG 248
              +    S   N T V  FNPC YAF +E   F F S  +E+ ++        V+LDW 
Sbjct: 186 SHRIETQPSRFENMTSVEHFNPCSYAFFVEDGMFNFSS--LEDLKDLRNVTRFPVLLDWS 245

Query: 249 IRNET------RFECGSNTTRSSILDGTEYRCQCLDGYEGNPYLPQGCQDVDECEYAWLN 287
           I N+T      R  CG N+T      G  Y C+CL G++GNPYL  GCQD++EC    ++
Sbjct: 246 IGNQTCEQVVGRNICGGNSTCFDSTRGKGYNCKCLQGFDGNPYLSDGCQDINECT-TRIH 305

BLAST of Csor.00g289080 vs. ExPASy Swiss-Prot
Match: Q39191 (Wall-associated receptor kinase 1 OS=Arabidopsis thaliana OX=3702 GN=WAK1 PE=1 SV=2)

HSP 1 Score: 138.7 bits (348), Expect = 1.3e-31
Identity = 96/315 (30.48%), Postives = 148/315 (46.98%), Query Frame = 0

Query: 23  MVASRALPG--CDEQCGDVQIPYPFGIKEGCYL--NQNFSITCNKTDRNGPPKAYLMQTN 82
           +V  +  PG  C  +CG++ I YPFGI  GCY   N++FSITC K DR        + ++
Sbjct: 21  LVKGQHQPGENCQNKCGNITIEYPFGISSGCYYPGNESFSITC-KEDRPH------VLSD 80

Query: 83  ISVTNISTNGELHVLQPVVRTCYDQVNGSFVPKESNLSVPAMFSISGTKNKFVTIGCNTV 142
           I V N + +G+L VL     TCYD+  G    ++S+ ++  + S+S   NK   +GCN +
Sbjct: 81  IEVANFNHSGQLQVLLNRSSTCYDE-QGKKTEEDSSFTLENL-SLS-ANNKLTAVGCNAL 140

Query: 143 GIIAGI------------------------SKGVC-MELEFPNGLTNLSLAVSGLLNFTD 202
            ++                            +G C +++  P            + + T 
Sbjct: 141 SLLDTFGMQNYSTACLSLCDSPPEADGECNGRGCCRVDVSAPLDSYTFETTSGRIKHMTS 200

Query: 203 VRTFNPCGYAFVIEADRFRFLS-------RYIENFEEEVEVVLDWGIRNETRFECGSNTT 262
              F+PC YAF++E D+F F S       R +  F     V+LDW + N+T  + GS + 
Sbjct: 201 FHDFSPCTYAFLVEDDKFNFSSTEDLLNLRNVMRF----PVLLDWSVGNQTCEQVGSTSI 260

Query: 263 ---RSSILDGTE---YRCQCLDGYEGNPYLPQGCQDVDECEYA---WLNDCEYKDKCANT 293
               S+ LD T    Y C+C +G++GNPYL  GCQDV+EC  +     ++C     C N 
Sbjct: 261 CGGNSTCLDSTPRNGYICRCNEGFDGNPYLSAGCQDVNECTTSSTIHRHNCSDPKTCRNK 320

BLAST of Csor.00g289080 vs. ExPASy Swiss-Prot
Match: Q9LMN8 (Wall-associated receptor kinase 3 OS=Arabidopsis thaliana OX=3702 GN=WAK3 PE=2 SV=2)

HSP 1 Score: 137.1 bits (344), Expect = 3.8e-31
Identity = 101/332 (30.42%), Postives = 152/332 (45.78%), Query Frame = 0

Query: 7   FMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYL--NQNFSITCNKT 66
           F+V+I  +A   T L     +    C  +CG+V I YPFGI  GCY   + NF++TC   
Sbjct: 8   FLVVIFFLAY--TQLVKGQHQPREDCKLKCGNVTIEYPFGISTGCYYPGDDNFNLTCVVE 67

Query: 67  DRNGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYDQ---VNGSFVPKESNLSVPAMF 126
           ++       L+   I VTNIS +G + VL      CY+Q    NG+ +       + + F
Sbjct: 68  EK------LLLFGIIQVTNISHSGHVSVLFERFSECYEQKNETNGTAL----GYQLGSSF 127

Query: 127 SISGTKNKFVTIGCNTVGIIAGISK------------------------GVCMELE---- 186
           S+S + NKF  +GCN + +++   K                        G C   +    
Sbjct: 128 SLS-SNNKFTLVGCNALSLLSTFGKQNYSTGCLSLCNSQPEANGRCNGVGCCTTEDFSVP 187

Query: 187 -----FPNGLTNLSLAVSGLLNF--TDVRTFNPCGYAFVIEADRFRF-LSRYIENFEE-- 246
                F  G   L   V+  L+   T V  FNPC YAF++E  +F F  S+ ++N     
Sbjct: 188 FDSDTFQFGSVRLRNQVNNSLDLFNTSVYQFNPCTYAFLVEDGKFNFDSSKDLKNLRNVT 247

Query: 247 EVEVVLDWGIRNETRFE------CGSNTTRSSILDGTEYRCQCLDGYEGNPYLPQGCQDV 290
              V LDW I N+T  +      CG N++  +      Y C+C +GY+GNPY  +GC+D+
Sbjct: 248 RFPVALDWSIGNQTCEQAGSTRICGKNSSCYNSTTRNGYICKCNEGYDGNPYRSEGCKDI 307

BLAST of Csor.00g289080 vs. ExPASy Swiss-Prot
Match: Q9LMN6 (Wall-associated receptor kinase 4 OS=Arabidopsis thaliana OX=3702 GN=WAK4 PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 1.1e-27
Identity = 96/327 (29.36%), Postives = 144/327 (44.04%), Query Frame = 0

Query: 1   MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT 60
           MK    F+V I  ++ +     +V  + LP C E+CG+V + YPFG   GC+  ++ S  
Sbjct: 1   MKVQRLFLVAIFCLSYMQ----LVKGQTLPRCPEKCGNVTLEYPFGFSPGCWRAEDPSFN 60

Query: 61  CNKTDRNGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYDQVNGSFVPKESNLSVPAM 120
            +  + N      L    + V  IS + +L VL P    CY+   G F       S    
Sbjct: 61  LSCVNEN------LFYKGLEVVEISHSSQLRVLYPASYICYNS-KGKFAKGTYYWSNLGN 120

Query: 121 FSISGTKNKFVTIGC-------------NTVGIIA----------GISKGV-CMELEFPN 180
            ++SG  N    +GC             N+VG I+          G   G  C +   P 
Sbjct: 121 LTLSG-NNTITALGCNSYAFVSSNGTRRNSVGCISACDALSHEANGECNGEGCCQNPVPA 180

Query: 181 GLTNLSLAVSGLLNFTDVRTFN--PCGYAFVIEADRFRFLS----RYIENFEEEVEVVLD 240
           G   L +      N T V+  +   C YAF++E  +F++ +     Y++N      VVLD
Sbjct: 181 GNNWLIVRSYRFDNDTSVQPISEGQCIYAFLVENGKFKYNASDKYSYLQNRNVGFPVVLD 240

Query: 241 WGIRNET-----RFECGSNTTRSSILDGTEYRCQCLDGYEGNPYLPQGCQDVDECEYA-- 290
           W IR ET       +CG N   S+   G  Y C+C  G++GNPYL  GCQD++EC  A  
Sbjct: 241 WSIRGETCGQVGEKKCGVNGICSNSASGIGYTCKCKGGFQGNPYLQNGCQDINECTTANP 300

BLAST of Csor.00g289080 vs. NCBI nr
Match: KAG6602139.1 (Wall-associated receptor kinase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 727 bits (1876), Expect = 8.71e-265
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0

Query: 1   MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT 60
           MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT
Sbjct: 1   MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT 60

Query: 61  CNKTDRNGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYDQVNGSFVPKESNLSVPAM 120
           CNKTDRNGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYDQVNGSFVPKESNLSVPAM
Sbjct: 61  CNKTDRNGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYDQVNGSFVPKESNLSVPAM 120

Query: 121 FSISGTKNKFVTIGCNTVGIIAGISKGVCMELEFPNGLTNLSLAVSGLLNFTDVRTFNPC 180
           FSISGTKNKFVTIGCNTVGIIAGISKGVCMELEFPNGLTNLSLAVSGLLNFTDVRTFNPC
Sbjct: 121 FSISGTKNKFVTIGCNTVGIIAGISKGVCMELEFPNGLTNLSLAVSGLLNFTDVRTFNPC 180

Query: 181 GYAFVIEADRFRFLSRYIENFEEEVEVVLDWGIRNETRFECGSNTTRSSILDGTEYRCQC 240
           GYAFVIEADRFRFLSRYIENFEEEVEVVLDWGIRNETRFECGSNTTRSSILDGTEYRCQC
Sbjct: 181 GYAFVIEADRFRFLSRYIENFEEEVEVVLDWGIRNETRFECGSNTTRSSILDGTEYRCQC 240

Query: 241 LDGYEGNPYLPQGCQDVDECEYAWLNDCEYKDKCANTKGNYTCHCPKNFHGDGREGGEGC 300
           LDGYEGNPYLPQGCQDVDECEYAWLNDCEYKDKCANTKGNYTCHCPKNFHGDGREGGEGC
Sbjct: 241 LDGYEGNPYLPQGCQDVDECEYAWLNDCEYKDKCANTKGNYTCHCPKNFHGDGREGGEGC 300

Query: 301 TKNSTSSIPIIIEETVYLLDGASESSQLVGSGSMGTVGNSIKLEITLMEHGR 352
           TKNSTSSIPIIIEETVYLLDGASESSQLVGSGSMGTVGNSIKLEITLMEHGR
Sbjct: 301 TKNSTSSIPIIIEETVYLLDGASESSQLVGSGSMGTVGNSIKLEITLMEHGR 352

BLAST of Csor.00g289080 vs. NCBI nr
Match: XP_022959563.1 (uncharacterized protein LOC111460594 [Cucurbita moschata])

HSP 1 Score: 588 bits (1517), Expect = 2.02e-193
Identity = 289/339 (85.25%), Postives = 299/339 (88.20%), Query Frame = 0

Query: 1   MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT 60
           MKHIMAFMVMIMEIAILSTALAMVAS+ALP CD++CGDV IPYPFGIKEGCYLNQNFSIT
Sbjct: 1   MKHIMAFMVMIMEIAILSTALAMVASQALPQCDDRCGDVHIPYPFGIKEGCYLNQNFSIT 60

Query: 61  CNKTDRNGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYDQVNGSFVPKESNLSVPAM 120
           CNKTDRNGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYDQVNGSFVP ESNLSVPAM
Sbjct: 61  CNKTDRNGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYDQVNGSFVPNESNLSVPAM 120

Query: 121 FSISGTKNKFVTIGCNTVGIIAGISKGV-------------------------CMELEFP 180
           FSISGTKNKFVTIGCNT+ +I G  +G                          C +LEFP
Sbjct: 121 FSISGTKNKFVTIGCNTLSVITGNHQGSLYGSGCISVCLNKSSVVNGPCSGNGCCQLEFP 180

Query: 181 NGLTNLSLAVSGLLNFTDVRTFNPCGYAFVIEAD-RFRFLSRYIENFE-EEVEVVLDWGI 240
           NGLTNLS+AV GLLNFTDVRTFNPCGYAFVIE + RF FLS YIENFE EEVEVVLDWGI
Sbjct: 181 NGLTNLSVAVGGLLNFTDVRTFNPCGYAFVIEEEKRFEFLSSYIENFEDEEVEVVLDWGI 240

Query: 241 RNETRFECGSNTTRSSILDGTEYRCQCLDGYEGNPYLPQGCQDVDECEYAWLNDCEYKDK 300
           RNETRFECGSNTTRSSILDGTEYRCQCLDGY+GNPYLPQGCQDVDECEYAWLNDCEYKDK
Sbjct: 241 RNETRFECGSNTTRSSILDGTEYRCQCLDGYDGNPYLPQGCQDVDECEYAWLNDCEYKDK 300

Query: 301 CANTKGNYTCHCPKNFHGDGREGGEGCTKNSTSSIPIII 312
           CANTKGNYTCHCPKNFHGDGREGGEGCTKNSTSSIPIII
Sbjct: 301 CANTKGNYTCHCPKNFHGDGREGGEGCTKNSTSSIPIII 339

BLAST of Csor.00g289080 vs. NCBI nr
Match: XP_022989771.1 (putative wall-associated receptor kinase-like 16 [Cucurbita maxima])

HSP 1 Score: 555 bits (1431), Expect = 3.03e-191
Identity = 273/338 (80.77%), Postives = 291/338 (86.09%), Query Frame = 0

Query: 1   MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT 60
           MKHIMAFMVM MEIAILSTALAMVAS+ALP CD++CGDVQIPYPFGIKEGCYLNQNFSIT
Sbjct: 1   MKHIMAFMVM-MEIAILSTALAMVASQALPRCDDRCGDVQIPYPFGIKEGCYLNQNFSIT 60

Query: 61  CNKTDRNGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYDQVNGSFVPKESNLSVPAM 120
           CNKTDRNGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYD+ NGSFVP ESN SVPAM
Sbjct: 61  CNKTDRNGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYDKANGSFVPNESNFSVPAM 120

Query: 121 FSISGTKNKFVTIGCNTVGIIAGI----------------SKGV---------CMELEFP 180
           F ISGTKNKFVTIGCNT G+IAG                   GV         C +LE P
Sbjct: 121 FPISGTKNKFVTIGCNTAGLIAGYLQESLYGSGCISLCLNKSGVVNGSCSGSGCCQLEIP 180

Query: 181 NGLTNLSLAVSGLLNFTDVRTFNPCGYAFVIEADRFRFLSRYIENFEEEVEVVLDWGIRN 240
           NGLTNLSLAV+GLLNF DVRTFN CGYAFVIEADRF+FLSRYIENFEEEVEVVLDWGIRN
Sbjct: 181 NGLTNLSLAVTGLLNFADVRTFNHCGYAFVIEADRFQFLSRYIENFEEEVEVVLDWGIRN 240

Query: 241 ETRFECGSNTTRS-SILDGTEYRCQCLDGYEGNPYLPQGCQDVDECEYAWLNDCEYKDKC 300
           ETR ECGSN  R+ SI +GT+YRCQCLDGYEGNPYL  GCQDVDEC+Y WLNDC++KDKC
Sbjct: 241 ETRIECGSNARRTNSIFNGTQYRCQCLDGYEGNPYLRHGCQDVDECKYPWLNDCKHKDKC 300

Query: 301 ANTKGNYTCHCPKNFHGDGREGGEGCTKNSTSSIPIII 312
           +NT+GNYTCHCPKNFHGDGR+GG+GCTKNSTSSIPIII
Sbjct: 301 SNTEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIII 337

BLAST of Csor.00g289080 vs. NCBI nr
Match: XP_023542638.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111802455 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 531 bits (1367), Expect = 1.44e-173
Identity = 265/337 (78.64%), Postives = 279/337 (82.79%), Query Frame = 0

Query: 1   MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT 60
           MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT
Sbjct: 1   MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT 60

Query: 61  CNKTDRNGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYDQVNGSFVPKESNLSVPAM 120
           CNKT              I++       +LHVLQPVVRTCYDQVNGSFVPKESNLS+PAM
Sbjct: 61  CNKTX-------------IAM-------DLHVLQPVVRTCYDQVNGSFVPKESNLSLPAM 120

Query: 121 FSISGTKNKFVTIGCNTVGIIAGISKGV-------------------------CMELEFP 180
           FSISGTKN+FVTIGCNT+ +IAG  +G                          C +LEFP
Sbjct: 121 FSISGTKNRFVTIGCNTLSVIAGNHQGSLYGSGCLSVCLNKSSVVNGPCSGNGCCQLEFP 180

Query: 181 NGLTNLSLAVSGLLNFTDVRTFNPCGYAFVIEADRFRFLSRYIENFEEEVEVVLDWGIRN 240
           NGLTNLSLAVSG LNFTDVRTFNPCGYAFVIEADRFRFLSRYIENFEEEVEVVLDWGIRN
Sbjct: 181 NGLTNLSLAVSGFLNFTDVRTFNPCGYAFVIEADRFRFLSRYIENFEEEVEVVLDWGIRN 240

Query: 241 ETRFECGSNTTRSSILDGTEYRCQCLDGYEGNPYLPQGCQDVDECEYAWLNDCEYKDKCA 300
           ETRFECGSNTTRSSILDGTEYRCQCLDGY+GNPYLPQGCQDV+EC+  WLNDC +K KC 
Sbjct: 241 ETRFECGSNTTRSSILDGTEYRCQCLDGYDGNPYLPQGCQDVNECKSGWLNDCMHKAKCI 300

Query: 301 NTKGNYTCHCPKNFHGDGREGGEGCTKNSTSSIPIII 312
           NT+GNYTC CPKNFHGDGREGGEGCTKNSTSSIPIII
Sbjct: 301 NTEGNYTCRCPKNFHGDGREGGEGCTKNSTSSIPIII 317

BLAST of Csor.00g289080 vs. NCBI nr
Match: KAG6602275.1 (Wall-associated receptor kinase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 461 bits (1186), Expect = 1.17e-159
Identity = 226/349 (64.76%), Postives = 265/349 (75.93%), Query Frame = 0

Query: 1   MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT 60
           MK IMAF+VM M IAILST LA+ AS+ALPGC+E+CGDV IPYPFGIKEGCYLNQNFSIT
Sbjct: 3   MKPIMAFLVM-MNIAILSTPLAVAASQALPGCEERCGDVHIPYPFGIKEGCYLNQNFSIT 62

Query: 61  CNKTDRNGPPKA--YLMQTNISVTNISTNGELHVLQPVVRTCYDQVNGSFVPKESNLSVP 120
           C+KTDRNGPPKA  +LM TNI V NISTNGELHVL+P+VR CY   +      E++LSVP
Sbjct: 63  CHKTDRNGPPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDDYLDMNETDLSVP 122

Query: 121 AMFSISGTKNKFVTIGCNTVGIIAGISKGV-------------------------CMELE 180
            M+ +S +KNKF+TIGCN VG++ G  +G                          C +L+
Sbjct: 123 TMYPLSRSKNKFITIGCNHVGLMLGDDQGTNVESGCISVCTRRSSVVDGLCSGSGCCQLD 182

Query: 181 FPNGLTNLSLAVSGLLNFTDVRTFNPCGYAFVIEADRFRFLSRYIENFEE-EVEVVLDWG 240
            P GLTNLSLAV  LLN+T++R F+PCGYAF+IEA RF+FLS YI+ FEE EVE VL WG
Sbjct: 183 IPKGLTNLSLAVGELLNYTEIRKFSPCGYAFIIEAKRFQFLSSYIDKFEEKEVEAVLSWG 242

Query: 241 IRNETRFECGSNTTRS-SILDGTEYRCQCLDGYEGNPYLPQGCQDVDECEYAWLNDCEYK 300
           IR E    CG N  R+ SI +GT YRC CLDGYEGNPYLP GCQDVDEC Y WLNDCE+K
Sbjct: 243 IRKELELACGLNARRNNSIFNGTHYRCHCLDGYEGNPYLPHGCQDVDECTYPWLNDCEHK 302

Query: 301 DKCANTKGNYTCHCPKNFHGDGREGGEGCTKNSTSSIPIIIEETVYLLD 320
           DKC+NT+GNYTCHCPKNFHGDGR+GG+GCT+N TSSIPIII E +Y+L+
Sbjct: 303 DKCSNTEGNYTCHCPKNFHGDGRKGGKGCTQNPTSSIPIIIGEFLYVLN 350

BLAST of Csor.00g289080 vs. ExPASy TrEMBL
Match: A0A6J1H6B0 (uncharacterized protein LOC111460594 OS=Cucurbita moschata OX=3662 GN=LOC111460594 PE=4 SV=1)

HSP 1 Score: 588 bits (1517), Expect = 9.80e-194
Identity = 289/339 (85.25%), Postives = 299/339 (88.20%), Query Frame = 0

Query: 1   MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT 60
           MKHIMAFMVMIMEIAILSTALAMVAS+ALP CD++CGDV IPYPFGIKEGCYLNQNFSIT
Sbjct: 1   MKHIMAFMVMIMEIAILSTALAMVASQALPQCDDRCGDVHIPYPFGIKEGCYLNQNFSIT 60

Query: 61  CNKTDRNGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYDQVNGSFVPKESNLSVPAM 120
           CNKTDRNGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYDQVNGSFVP ESNLSVPAM
Sbjct: 61  CNKTDRNGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYDQVNGSFVPNESNLSVPAM 120

Query: 121 FSISGTKNKFVTIGCNTVGIIAGISKGV-------------------------CMELEFP 180
           FSISGTKNKFVTIGCNT+ +I G  +G                          C +LEFP
Sbjct: 121 FSISGTKNKFVTIGCNTLSVITGNHQGSLYGSGCISVCLNKSSVVNGPCSGNGCCQLEFP 180

Query: 181 NGLTNLSLAVSGLLNFTDVRTFNPCGYAFVIEAD-RFRFLSRYIENFE-EEVEVVLDWGI 240
           NGLTNLS+AV GLLNFTDVRTFNPCGYAFVIE + RF FLS YIENFE EEVEVVLDWGI
Sbjct: 181 NGLTNLSVAVGGLLNFTDVRTFNPCGYAFVIEEEKRFEFLSSYIENFEDEEVEVVLDWGI 240

Query: 241 RNETRFECGSNTTRSSILDGTEYRCQCLDGYEGNPYLPQGCQDVDECEYAWLNDCEYKDK 300
           RNETRFECGSNTTRSSILDGTEYRCQCLDGY+GNPYLPQGCQDVDECEYAWLNDCEYKDK
Sbjct: 241 RNETRFECGSNTTRSSILDGTEYRCQCLDGYDGNPYLPQGCQDVDECEYAWLNDCEYKDK 300

Query: 301 CANTKGNYTCHCPKNFHGDGREGGEGCTKNSTSSIPIII 312
           CANTKGNYTCHCPKNFHGDGREGGEGCTKNSTSSIPIII
Sbjct: 301 CANTKGNYTCHCPKNFHGDGREGGEGCTKNSTSSIPIII 339

BLAST of Csor.00g289080 vs. ExPASy TrEMBL
Match: A0A6J1JL32 (putative wall-associated receptor kinase-like 16 OS=Cucurbita maxima OX=3661 GN=LOC111486859 PE=4 SV=1)

HSP 1 Score: 555 bits (1431), Expect = 1.47e-191
Identity = 273/338 (80.77%), Postives = 291/338 (86.09%), Query Frame = 0

Query: 1   MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT 60
           MKHIMAFMVM MEIAILSTALAMVAS+ALP CD++CGDVQIPYPFGIKEGCYLNQNFSIT
Sbjct: 1   MKHIMAFMVM-MEIAILSTALAMVASQALPRCDDRCGDVQIPYPFGIKEGCYLNQNFSIT 60

Query: 61  CNKTDRNGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYDQVNGSFVPKESNLSVPAM 120
           CNKTDRNGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYD+ NGSFVP ESN SVPAM
Sbjct: 61  CNKTDRNGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYDKANGSFVPNESNFSVPAM 120

Query: 121 FSISGTKNKFVTIGCNTVGIIAGI----------------SKGV---------CMELEFP 180
           F ISGTKNKFVTIGCNT G+IAG                   GV         C +LE P
Sbjct: 121 FPISGTKNKFVTIGCNTAGLIAGYLQESLYGSGCISLCLNKSGVVNGSCSGSGCCQLEIP 180

Query: 181 NGLTNLSLAVSGLLNFTDVRTFNPCGYAFVIEADRFRFLSRYIENFEEEVEVVLDWGIRN 240
           NGLTNLSLAV+GLLNF DVRTFN CGYAFVIEADRF+FLSRYIENFEEEVEVVLDWGIRN
Sbjct: 181 NGLTNLSLAVTGLLNFADVRTFNHCGYAFVIEADRFQFLSRYIENFEEEVEVVLDWGIRN 240

Query: 241 ETRFECGSNTTRS-SILDGTEYRCQCLDGYEGNPYLPQGCQDVDECEYAWLNDCEYKDKC 300
           ETR ECGSN  R+ SI +GT+YRCQCLDGYEGNPYL  GCQDVDEC+Y WLNDC++KDKC
Sbjct: 241 ETRIECGSNARRTNSIFNGTQYRCQCLDGYEGNPYLRHGCQDVDECKYPWLNDCKHKDKC 300

Query: 301 ANTKGNYTCHCPKNFHGDGREGGEGCTKNSTSSIPIII 312
           +NT+GNYTCHCPKNFHGDGR+GG+GCTKNSTSSIPIII
Sbjct: 301 SNTEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIII 337

BLAST of Csor.00g289080 vs. ExPASy TrEMBL
Match: A0A6J1JGR1 (wall-associated receptor kinase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111486860 PE=4 SV=1)

HSP 1 Score: 457 bits (1177), Expect = 3.05e-153
Identity = 225/340 (66.18%), Postives = 259/340 (76.18%), Query Frame = 0

Query: 2   KHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSITC 61
           K IMAFMVM+M+I ILSTALA+ AS ALPGCDE CG V IPYPFGIK+GCYLNQNFSITC
Sbjct: 6   KPIMAFMVMMMKIGILSTALAVEASEALPGCDEWCGKVHIPYPFGIKKGCYLNQNFSITC 65

Query: 62  NKTDRNGPPKA--YLMQTNISVTNISTNGELHVLQPVVRTCYDQVNGSFVPKESNLSVPA 121
           +KTD NGPPKA  +LM TNI V NISTNGELHVL+P+VR CY   +      E++LSVP 
Sbjct: 66  HKTDLNGPPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETDLSVPT 125

Query: 122 MFSISGTKNKFVTIGCNTVGIIAGISKGV-------------------------CMELEF 181
           M+ +S +KNKF+TIGCN VG+I G  +G                          C +LE 
Sbjct: 126 MYPLSRSKNKFITIGCNHVGLILGDDQGTNVESGCISVCTRRISVVDGLCSGSGCCQLEI 185

Query: 182 PNGLTNLSLAVSGLLNFTDVRTFNPCGYAFVIEADRFRFLSRYIENFEE-EVEVVLDWGI 241
           P GLT LSLAV  LLN+T++R F+PCGYAF+IEA RF+FLS YI+ FEE EVEVVL WGI
Sbjct: 186 PKGLTKLSLAVGELLNYTEIRKFSPCGYAFIIEARRFQFLSSYIDKFEEKEVEVVLSWGI 245

Query: 242 RNETRFECGSNTTR-SSILDGTEYRCQCLDGYEGNPYLPQGCQDVDECEYAWLNDCEYKD 301
           R E   +CG N  R SSI +GT YRCQCLDGYEGNPYL  GCQDVDEC+Y WLNDC++KD
Sbjct: 246 RKELELDCGLNARRNSSIFNGTHYRCQCLDGYEGNPYLRHGCQDVDECKYPWLNDCKHKD 305

Query: 302 KCANTKGNYTCHCPKNFHGDGREGGEGCTKNSTSSIPIII 312
           KC+NT+GNYTCHCPKNFHGDGR+GG+GCTKNSTSSIPIII
Sbjct: 306 KCSNTEGNYTCHCPKNFHGDGRKGGKGCTKNSTSSIPIII 345

BLAST of Csor.00g289080 vs. ExPASy TrEMBL
Match: A0A6J1H8D6 (uncharacterized protein LOC111460588 OS=Cucurbita moschata OX=3662 GN=LOC111460588 PE=4 SV=1)

HSP 1 Score: 458 bits (1178), Expect = 2.49e-144
Identity = 225/338 (66.57%), Postives = 256/338 (75.74%), Query Frame = 0

Query: 1    MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT 60
            MK IMAF+VM M IAILST LA+ AS+ALPGC+E+CGDV IPYPFGIKEGCYLNQNFSI 
Sbjct: 1098 MKPIMAFLVM-MNIAILSTPLAVAASQALPGCEERCGDVHIPYPFGIKEGCYLNQNFSIA 1157

Query: 61   CNKTDRNGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYDQVNGSFVPKESNLSVPAM 120
            C+KTDRNGPPKA+LM TNISV NI TNGELHVLQP+VR CY          +S LSVP M
Sbjct: 1158 CDKTDRNGPPKAFLMHTNISVMNIFTNGELHVLQPIVRNCYSDGGSYLDMNDSYLSVPDM 1217

Query: 121  FSISGTKNKFVTIGCNTVGIIAG-------------------------ISKGVCMELEFP 180
            + +S +KNKF+TIGCN VG+I G                          S   C +LE P
Sbjct: 1218 YPLSRSKNKFITIGCNHVGLILGDYLGGDFESGCISMCTKKSSVADGSCSGSGCCQLEIP 1277

Query: 181  NGLTNLSLAVSGLLNFTDVRTFNPCGYAFVIEADRFRFLSRYIENFEEE-VEVVLDWGIR 240
             GLTNL LAV  LLN+T++   +PCGYAF+IEA+RFRFLS YI+ FE+E V VVL WGIR
Sbjct: 1278 KGLTNLRLAVGELLNYTEIPKLSPCGYAFIIEAERFRFLSSYIDKFEDEKVVVVLSWGIR 1337

Query: 241  NETRFECGSNTTRSSILDGTEYRCQCLDGYEGNPYLPQGCQDVDECEYAWLNDCEYKDKC 300
            NE +FECGSNT R SI +GT+Y CQCLDGYEGNPYL  GCQDV+EC+Y WLNDC +K KC
Sbjct: 1338 NELKFECGSNTARISIFNGTQYGCQCLDGYEGNPYLRHGCQDVNECKYGWLNDCMHKAKC 1397

Query: 301  ANTKGNYTCHCPKNFHGDGREGGEGCTKNSTSSIPIII 312
             NT+GNYTC CPKNFHGDGR+GGEGCTKNSTSSIPIII
Sbjct: 1398 INTEGNYTCRCPKNFHGDGRKGGEGCTKNSTSSIPIII 1434

BLAST of Csor.00g289080 vs. ExPASy TrEMBL
Match: A0A5D3D516 (Wall-associated receptor kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold390G00070 PE=4 SV=1)

HSP 1 Score: 375 bits (964), Expect = 1.91e-122
Identity = 187/330 (56.67%), Postives = 229/330 (69.39%), Query Frame = 0

Query: 14  IAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSITCNKTDRNGPPKAY 73
           + ILST + + +S+ALPGCDE CGDVQIPYPFG+K+GCYLNQ FSITC+KT    PPKA+
Sbjct: 13  VIILSTTIVVASSQALPGCDEWCGDVQIPYPFGVKQGCYLNQTFSITCSKTI--SPPKAF 72

Query: 74  LMQTNISVTNISTNGELHVLQPVVRTCYDQ--VNGSFVPKESNLSVPAMFSISGTKNKFV 133
           LM TNISVTNIS NGELH+ QP+VR CY Q  V GS VP  ++L VPAMF I+  KNKF+
Sbjct: 73  LMNTNISVTNISLNGELHISQPIVRDCYRQGIVIGSSVPTATDLLVPAMFPIADGKNKFI 132

Query: 134 TIGCNTVGIIAGISKGV-------------------------CMELEFPNGLTNLSLAVS 193
            IGC+T G+I G+  G                          C ELE PNGL NL L V 
Sbjct: 133 AIGCDTFGLIGGVLNGSGYVSGCISMCLNESMIGNEKCLGNGCCELEIPNGLMNLKLLVG 192

Query: 194 GLLNFTDVRTFNPCGYAFVIEADRFRFLSRYIENFEE-EVEVVLDWGIRNETRFECGSNT 253
              N + V+ FNPCGYAFV+  + F F S+Y+ +F++ EVEVV+ W I NE+ + CG N+
Sbjct: 193 SFFNHSLVKDFNPCGYAFVVGNEGFEFDSKYVRSFQDVEVEVVVGWAIGNESNYVCGLNS 252

Query: 254 TRSSIL--DGTEYRCQCLDGYEGNPYLPQGCQDVDECEYAWLNDCEYKDKCANTKGNYTC 312
            R+     DG E+RCQCL+G++GNPYLPQGCQD+DEC+   LN C+YK KC NT GNYTC
Sbjct: 253 KRNYNFSNDGREFRCQCLEGFQGNPYLPQGCQDIDECKDETLNQCKYKSKCVNTIGNYTC 312

BLAST of Csor.00g289080 vs. TAIR 10
Match: AT1G21270.1 (wall-associated kinase 2 )

HSP 1 Score: 162.2 bits (409), Expect = 7.8e-40
Identity = 96/295 (32.54%), Postives = 144/295 (48.81%), Query Frame = 0

Query: 32  CDEQCGDVQIPYPFGIKEGCYL--NQNFSITCNKTDRNGPPKAYLMQTNISVTNISTNGE 91
           C  +CG+V + YPFG   GCY   +++F++TCN+ ++       L   N+ V N+S +G+
Sbjct: 29  CQTRCGNVAVEYPFGTSPGCYYPGDESFNLTCNEQEK-------LFFGNMPVINMSLSGQ 88

Query: 92  LHVLQPVVRTCYDQVNGSFVPKESNLSVPAMFSISGTKNKFVTIGCNTVGII--AGISK- 151
           L V     R CYD   G      +  +    F++S   N+F  +GCN+   +  +G+ K 
Sbjct: 89  LRVRLVRSRVCYDS-QGKQTDYIAQRTTLGNFTLS-ELNRFTVVGCNSYAFLRTSGVEKY 148

Query: 152 --------------------GVCMELEFPNGLTNLSLAVSGLLNFTDVRTFNPCGYAFVI 211
                                 C ++  P G + + +      N   V  FNPC YAF++
Sbjct: 149 STGCISICDSATTKNGSCSGEGCCQIPVPRGYSFVRVKPHSFHNHPTVHLFNPCTYAFLV 208

Query: 212 EADRFRFLSRYIENFEEEV---EVVLDWGIRNET------RFECGSNTTRSSILDGTEYR 271
           E   F F +    N    V    VVLDW I ++T      R  CG N+T      GT Y 
Sbjct: 209 EDGMFDFHALEDLNNLRNVTTFPVVLDWSIGDKTCKQVEYRGVCGGNSTCFDSTGGTGYN 268

Query: 272 CQCLDGYEGNPYLPQGCQDVDECEYAWLNDCEYKDKCANTKGNYTCHCPKNFHGD 293
           C+CL+G+EGNPYLP GCQD++EC  +  ++C     C NTKG++ C+CP  +  D
Sbjct: 269 CKCLEGFEGNPYLPNGCQDINEC-ISSRHNCSEHSTCENTKGSFNCNCPSGYRKD 313

BLAST of Csor.00g289080 vs. TAIR 10
Match: AT1G21230.1 (wall associated kinase 5 )

HSP 1 Score: 152.5 bits (384), Expect = 6.2e-37
Identity = 104/320 (32.50%), Postives = 144/320 (45.00%), Query Frame = 0

Query: 9   VMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYL--NQNFSITCNKTDR 68
           + +M I        +V ++    C  +CGDV I YPFGI  GCY   + +F+ITC +   
Sbjct: 6   LFLMAIFFYLAYTQLVKAQPRDDCQTRCGDVPIDYPFGISTGCYYPGDDSFNITCEEDKP 65

Query: 69  NGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYD-QVNGSFVP---KESNLSVPAMFS 128
           N       + +NI V N + +G+L  L P    CYD Q N  F     +  NLS      
Sbjct: 66  N-------VLSNIEVLNFNHSGQLRGLIPRSTVCYDQQTNNDFESLWFRLDNLSF----- 125

Query: 129 ISGTKNKFVTIGCNTVGIIA--GISK----------------------GVC-MELEFPNG 188
                NKF  +GCN   +++  GI                        G C  E+  P  
Sbjct: 126 --SPNNKFTLVGCNAWALLSTFGIQNYSTGCMSLCDTPPPPNSKCNGVGCCRTEVSIPLD 185

Query: 189 LTNLSLAVSGLLNFTDVRTFNPCGYAFVIEADRFRFLSRYIENFEE-----EVEVVLDWG 248
              +    S   N T V  FNPC YAF +E   F F S  +E+ ++        V+LDW 
Sbjct: 186 SHRIETQPSRFENMTSVEHFNPCSYAFFVEDGMFNFSS--LEDLKDLRNVTRFPVLLDWS 245

Query: 249 IRNET------RFECGSNTTRSSILDGTEYRCQCLDGYEGNPYLPQGCQDVDECEYAWLN 287
           I N+T      R  CG N+T      G  Y C+CL G++GNPYL  GCQD++EC    ++
Sbjct: 246 IGNQTCEQVVGRNICGGNSTCFDSTRGKGYNCKCLQGFDGNPYLSDGCQDINECT-TRIH 305

BLAST of Csor.00g289080 vs. TAIR 10
Match: AT1G21250.1 (cell wall-associated kinase )

HSP 1 Score: 138.7 bits (348), Expect = 9.3e-33
Identity = 96/315 (30.48%), Postives = 148/315 (46.98%), Query Frame = 0

Query: 23  MVASRALPG--CDEQCGDVQIPYPFGIKEGCYL--NQNFSITCNKTDRNGPPKAYLMQTN 82
           +V  +  PG  C  +CG++ I YPFGI  GCY   N++FSITC K DR        + ++
Sbjct: 21  LVKGQHQPGENCQNKCGNITIEYPFGISSGCYYPGNESFSITC-KEDRPH------VLSD 80

Query: 83  ISVTNISTNGELHVLQPVVRTCYDQVNGSFVPKESNLSVPAMFSISGTKNKFVTIGCNTV 142
           I V N + +G+L VL     TCYD+  G    ++S+ ++  + S+S   NK   +GCN +
Sbjct: 81  IEVANFNHSGQLQVLLNRSSTCYDE-QGKKTEEDSSFTLENL-SLS-ANNKLTAVGCNAL 140

Query: 143 GIIAGI------------------------SKGVC-MELEFPNGLTNLSLAVSGLLNFTD 202
            ++                            +G C +++  P            + + T 
Sbjct: 141 SLLDTFGMQNYSTACLSLCDSPPEADGECNGRGCCRVDVSAPLDSYTFETTSGRIKHMTS 200

Query: 203 VRTFNPCGYAFVIEADRFRFLS-------RYIENFEEEVEVVLDWGIRNETRFECGSNTT 262
              F+PC YAF++E D+F F S       R +  F     V+LDW + N+T  + GS + 
Sbjct: 201 FHDFSPCTYAFLVEDDKFNFSSTEDLLNLRNVMRF----PVLLDWSVGNQTCEQVGSTSI 260

Query: 263 ---RSSILDGTE---YRCQCLDGYEGNPYLPQGCQDVDECEYA---WLNDCEYKDKCANT 293
               S+ LD T    Y C+C +G++GNPYL  GCQDV+EC  +     ++C     C N 
Sbjct: 261 CGGNSTCLDSTPRNGYICRCNEGFDGNPYLSAGCQDVNECTTSSTIHRHNCSDPKTCRNK 320

BLAST of Csor.00g289080 vs. TAIR 10
Match: AT1G21240.1 (wall associated kinase 3 )

HSP 1 Score: 137.1 bits (344), Expect = 2.7e-32
Identity = 101/332 (30.42%), Postives = 152/332 (45.78%), Query Frame = 0

Query: 7   FMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYL--NQNFSITCNKT 66
           F+V+I  +A   T L     +    C  +CG+V I YPFGI  GCY   + NF++TC   
Sbjct: 8   FLVVIFFLAY--TQLVKGQHQPREDCKLKCGNVTIEYPFGISTGCYYPGDDNFNLTCVVE 67

Query: 67  DRNGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYDQ---VNGSFVPKESNLSVPAMF 126
           ++       L+   I VTNIS +G + VL      CY+Q    NG+ +       + + F
Sbjct: 68  EK------LLLFGIIQVTNISHSGHVSVLFERFSECYEQKNETNGTAL----GYQLGSSF 127

Query: 127 SISGTKNKFVTIGCNTVGIIAGISK------------------------GVCMELE---- 186
           S+S + NKF  +GCN + +++   K                        G C   +    
Sbjct: 128 SLS-SNNKFTLVGCNALSLLSTFGKQNYSTGCLSLCNSQPEANGRCNGVGCCTTEDFSVP 187

Query: 187 -----FPNGLTNLSLAVSGLLNF--TDVRTFNPCGYAFVIEADRFRF-LSRYIENFEE-- 246
                F  G   L   V+  L+   T V  FNPC YAF++E  +F F  S+ ++N     
Sbjct: 188 FDSDTFQFGSVRLRNQVNNSLDLFNTSVYQFNPCTYAFLVEDGKFNFDSSKDLKNLRNVT 247

Query: 247 EVEVVLDWGIRNETRFE------CGSNTTRSSILDGTEYRCQCLDGYEGNPYLPQGCQDV 290
              V LDW I N+T  +      CG N++  +      Y C+C +GY+GNPY  +GC+D+
Sbjct: 248 RFPVALDWSIGNQTCEQAGSTRICGKNSSCYNSTTRNGYICKCNEGYDGNPYRSEGCKDI 307

BLAST of Csor.00g289080 vs. TAIR 10
Match: AT1G21210.1 (wall associated kinase 4 )

HSP 1 Score: 125.6 bits (314), Expect = 8.1e-29
Identity = 96/327 (29.36%), Postives = 144/327 (44.04%), Query Frame = 0

Query: 1   MKHIMAFMVMIMEIAILSTALAMVASRALPGCDEQCGDVQIPYPFGIKEGCYLNQNFSIT 60
           MK    F+V I  ++ +     +V  + LP C E+CG+V + YPFG   GC+  ++ S  
Sbjct: 1   MKVQRLFLVAIFCLSYMQ----LVKGQTLPRCPEKCGNVTLEYPFGFSPGCWRAEDPSFN 60

Query: 61  CNKTDRNGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYDQVNGSFVPKESNLSVPAM 120
            +  + N      L    + V  IS + +L VL P    CY+   G F       S    
Sbjct: 61  LSCVNEN------LFYKGLEVVEISHSSQLRVLYPASYICYNS-KGKFAKGTYYWSNLGN 120

Query: 121 FSISGTKNKFVTIGC-------------NTVGIIA----------GISKGV-CMELEFPN 180
            ++SG  N    +GC             N+VG I+          G   G  C +   P 
Sbjct: 121 LTLSG-NNTITALGCNSYAFVSSNGTRRNSVGCISACDALSHEANGECNGEGCCQNPVPA 180

Query: 181 GLTNLSLAVSGLLNFTDVRTFN--PCGYAFVIEADRFRFLS----RYIENFEEEVEVVLD 240
           G   L +      N T V+  +   C YAF++E  +F++ +     Y++N      VVLD
Sbjct: 181 GNNWLIVRSYRFDNDTSVQPISEGQCIYAFLVENGKFKYNASDKYSYLQNRNVGFPVVLD 240

Query: 241 WGIRNET-----RFECGSNTTRSSILDGTEYRCQCLDGYEGNPYLPQGCQDVDECEYA-- 290
           W IR ET       +CG N   S+   G  Y C+C  G++GNPYL  GCQD++EC  A  
Sbjct: 241 WSIRGETCGQVGEKKCGVNGICSNSASGIGYTCKCKGGFQGNPYLQNGCQDINECTTANP 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LMP11.1e-3832.54Wall-associated receptor kinase 2 OS=Arabidopsis thaliana OX=3702 GN=WAK2 PE=1 S... [more]
Q9LMN78.7e-3632.50Wall-associated receptor kinase 5 OS=Arabidopsis thaliana OX=3702 GN=WAK5 PE=2 S... [more]
Q391911.3e-3130.48Wall-associated receptor kinase 1 OS=Arabidopsis thaliana OX=3702 GN=WAK1 PE=1 S... [more]
Q9LMN83.8e-3130.42Wall-associated receptor kinase 3 OS=Arabidopsis thaliana OX=3702 GN=WAK3 PE=2 S... [more]
Q9LMN61.1e-2729.36Wall-associated receptor kinase 4 OS=Arabidopsis thaliana OX=3702 GN=WAK4 PE=2 S... [more]
Match NameE-valueIdentityDescription
KAG6602139.18.71e-265100.00Wall-associated receptor kinase 2, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022959563.12.02e-19385.25uncharacterized protein LOC111460594 [Cucurbita moschata][more]
XP_022989771.13.03e-19180.77putative wall-associated receptor kinase-like 16 [Cucurbita maxima][more]
XP_023542638.11.44e-17378.64LOW QUALITY PROTEIN: uncharacterized protein LOC111802455 [Cucurbita pepo subsp.... [more]
KAG6602275.11.17e-15964.76Wall-associated receptor kinase 2, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
A0A6J1H6B09.80e-19485.25uncharacterized protein LOC111460594 OS=Cucurbita moschata OX=3662 GN=LOC1114605... [more]
A0A6J1JL321.47e-19180.77putative wall-associated receptor kinase-like 16 OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1JGR13.05e-15366.18wall-associated receptor kinase 3-like OS=Cucurbita maxima OX=3661 GN=LOC1114868... [more]
A0A6J1H8D62.49e-14466.57uncharacterized protein LOC111460588 OS=Cucurbita moschata OX=3662 GN=LOC1114605... [more]
A0A5D3D5161.91e-12256.67Wall-associated receptor kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
Match NameE-valueIdentityDescription
AT1G21270.17.8e-4032.54wall-associated kinase 2 [more]
AT1G21230.16.2e-3732.50wall associated kinase 5 [more]
AT1G21250.19.3e-3330.48cell wall-associated kinase [more]
AT1G21240.12.7e-3230.42wall associated kinase 3 [more]
AT1G21210.18.1e-2929.36wall associated kinase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001881EGF-like calcium-binding domainSMARTSM00179egfca_6coord: 256..301
e-value: 0.0017
score: 24.1
IPR001881EGF-like calcium-binding domainPFAMPF07645EGF_CAcoord: 256..293
e-value: 3.9E-6
score: 27.0
IPR025287Wall-associated receptor kinase, galacturonan-binding domainPFAMPF13947GUB_WAK_bindcoord: 32..88
e-value: 2.2E-10
score: 40.8
NoneNo IPR availableGENE3D2.10.25.10Laminincoord: 215..300
e-value: 1.1E-10
score: 43.7
NoneNo IPR availablePANTHERPTHR33491OSJNBA0016N04.9 PROTEINcoord: 6..261
NoneNo IPR availablePANTHERPTHR33491:SF5OSJNBA0016N04.9 PROTEINcoord: 6..261
NoneNo IPR availableCDDcd00054EGF_CAcoord: 256..292
e-value: 5.11454E-4
score: 35.305
NoneNo IPR availableSUPERFAMILY57196EGF/Laminincoord: 255..293
IPR000152EGF-type aspartate/asparagine hydroxylation sitePROSITEPS00010ASX_HYDROXYLcoord: 274..285
IPR018097EGF-like calcium-binding, conserved sitePROSITEPS01187EGF_CAcoord: 256..283
IPR000742EGF-like domainPROSITEPS50026EGF_3coord: 256..295
score: 12.171673

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g289080.m01Csor.00g289080.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0030247 polysaccharide binding
molecular_function GO:0004674 protein serine/threonine kinase activity