Csor.00g283160 (gene) Silver-seed gourd (wild; sororia) v1

BLAST of Csor.00g283160 vs. ExPASy Swiss-Prot
Match: Q9MAP5 (Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 1.4e-235
Identity = 414/747 (55.42%), Postives = 535/747 (71.62%), Query Frame = 0

Query: 18  RVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAK 77
           R   +S V+IVY+G+K H + E + ++HH +LAS+LGS++ + DS+VYSY++GFSGFAAK
Sbjct: 25  RSETESKVHIVYLGEKKHHDPEFVTESHHQMLASLLGSKKDADDSMVYSYRHGFSGFAAK 84

Query: 78  LTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVL 137
           LT +QA+ I++LP VV VIP+  H++ TTR+W+YL LS   P +LL  + MG   IIGV+
Sbjct: 85  LTKSQAKKIADLPEVVHVIPDGFHELATTRTWEYLGLSSANPKNLLNDTNMGDQVIIGVI 144

Query: 138 DTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVKGLEAAYGHP 197
           DTG+WPESE F D G+GP+P +WKG CESGE F  +  C+RKLIGA+YF+ G   A    
Sbjct: 145 DTGVWPESESFNDNGVGPIPRKWKGGCESGENFR-STDCNRKLIGAKYFINGF-LAENKG 204

Query: 198 LNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCW 257
            N +E +DY S RD  GHGTHV+S+A G FVPNVSY GLA GT+RGGAP +RIAMYK CW
Sbjct: 205 FNTTESRDYISARDFDGHGTHVASIAGGSFVPNVSYKGLAGGTLRGGAPRARIAMYKACW 264

Query: 258 ---QVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAK 317
              ++ G  C+D DI+KAID+AI+DGVDVLS+SL    P  S+ D+R+  A G FHAVAK
Sbjct: 265 FHEELKGVTCSDSDIMKAIDEAIHDGVDVLSISLVGQIPLNSETDIRDEFATGLFHAVAK 324

Query: 318 GIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQGMFSGKLT 377
           GIVVV A GN GPAA +V NI PW+LTVAA+++DRSF   ITLGNN   +GQ  ++G   
Sbjct: 325 GIVVVCAGGNDGPAAQTVVNIAPWILTVAATTLDRSFPTPITLGNNKVILGQATYTGPEL 384

Query: 378 KFHNLVYPEVSDLDDER---ACESLSLNDTWA-AGNVVLCFASDDYNDDTHNTSSSVKKV 437
              +LVYPE +  ++E     CESL+LN  +  A  VVLCF +   N      +S VK  
Sbjct: 385 GLTSLVYPENARNNNETFSGVCESLNLNPNYTMAMKVVLCFTASRTNAAISRAASFVKAA 444

Query: 438 GGLGLIVAKNPTKAVEPFVNNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVGQPL 497
           GGLGLI+++NP   + P  ++FPCV +  ++G  IL+YIRSTR+P VKI  S T  GQP+
Sbjct: 445 GGLGLIISRNPVYTLSPCNDDFPCVAVDYELGTDILSYIRSTRSPVVKIQRSRTLSGQPV 504

Query: 498 STTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATPHVS 557
            T V  FSSRGPNS++PAILKPDIAAPGV ILAA  P+D  +   +A +SGTSMATP +S
Sbjct: 505 GTKVVNFSSRGPNSMSPAILKPDIAAPGVRILAATSPNDTLNVGGFAMLSGTSMATPVIS 564

Query: 558 AIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVNPNK 617
            ++ALLK LH  WSPAA +SAIVTTAW +DP+G  IFAEG     + PFD+GGGIVNP K
Sbjct: 565 GVIALLKALHPEWSPAAFRSAIVTTAWRTDPFGEQIFAEGSSRKVSDPFDYGGGIVNPEK 624

Query: 618 AVDPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIPSLT 677
           A +PGL+YDMG  DYI Y C+ GYN+S+IS +  +   C   +PS+LD+N+PSITIP+L 
Sbjct: 625 AAEPGLIYDMGPQDYILYLCSAGYNDSSISQLVGQITVCSNPKPSVLDVNLPSITIPNLK 684

Query: 678 HPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNHRVT 737
             V+LTRTVTNVG ++S YK ++E P G+ + + P  L FN K  S+SFTV +S+ H++ 
Sbjct: 685 DEVTLTRTVTNVGLVDSVYKVSVEPPLGVRVVVTPETLVFNSKTISVSFTVRVSTTHKIN 744

Query: 738 TGYCFGSLTWLDGVHSVRIPVSVRTNI 758
           TGY FGSLTW D VH+V IP+SVRT I
Sbjct: 745 TGYYFGSLTWTDSVHNVVIPLSVRTQI 769

BLAST of Csor.00g283160 vs. ExPASy Swiss-Prot
Match: Q9ZSB0 (Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 SV=1)

HSP 1 Score: 813.5 bits (2100), Expect = 2.0e-234
Identity = 421/740 (56.89%), Postives = 540/740 (72.97%), Query Frame = 0

Query: 19  VAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKL 78
           V A+S VY+VY+G+K HDN E + ++HH +L S+LGS+E  +DS+VYSY++GFSGFAAKL
Sbjct: 23  VVAESKVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAKL 82

Query: 79  TMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLD 138
           T +QAQ ISELP VV+VIPN L++M TTR+WDYL +SP   +SLL+K+ MG   I+GV+D
Sbjct: 83  TESQAQQISELPEVVQVIPNTLYEMTTTRTWDYLGVSPGNSDSLLQKANMGYNVIVGVID 142

Query: 139 TGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPL 198
           +G+WPESE+F D+G GP+PSRWKG CESGELF+ +  C+RKLIGA+YFV GL A +G  +
Sbjct: 143 SGVWPESEMFNDKGFGPIPSRWKGGCESGELFNASIHCNRKLIGAKYFVDGLVAEFG-VV 202

Query: 199 NNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQ 258
           N ++  +Y SPRD +GHGTHV+S   G F+PNVSY GL  GT RGGAP   IA+YK CW 
Sbjct: 203 NRTQNPEYLSPRDFAGHGTHVASTIGGSFLPNVSYVGLGRGTARGGAPGVHIAVYKACW- 262

Query: 259 VNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVV 318
              G C+  D+LKA+D+AI+DGVD+LSLSLGPS P + + +     ++GAFHAVAKGI V
Sbjct: 263 --SGYCSGADVLKAMDEAIHDGVDILSLSLGPSVPLFPETE---HTSVGAFHAVAKGIPV 322

Query: 319 VGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHN 378
           V AAGN+GP A ++SN+ PW+LTVAA++ DRSF  AITLGNN T +GQ ++ G    F  
Sbjct: 323 VIAAGNAGPTAQTISNVAPWVLTVAATTQDRSFPTAITLGNNITILGQAIYGGPELGFVG 382

Query: 379 LVYPEVSDLDDERACESLSLN-DTWAAGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIV 438
           L YPE S L  +  CE LS N ++   G VVLCFA+   +  ++   ++V   GGLGLI+
Sbjct: 383 LTYPE-SPLSGD--CEKLSANPNSTMEGKVVLCFAA---STPSNAAIAAVINAGGLGLIM 442

Query: 439 AKNPTKAVEPFVNNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYF 498
           AKNPT ++ P    FP V I  ++G  IL YIRSTR+P VKI AS T  GQ +ST VA F
Sbjct: 443 AKNPTHSLTP-TRKFPWVSIDFELGTDILFYIRSTRSPIVKIQASKTLFGQSVSTKVATF 502

Query: 499 SSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLK 558
           SSRGPNSV+PAILKPDIAAPGV ILAA+ P+   +   +A +SGTSMATP VS +V LLK
Sbjct: 503 SSRGPNSVSPAILKPDIAAPGVNILAAISPNSSINDGGFAMMSGTSMATPVVSGVVVLLK 562

Query: 559 TLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLV 618
           +LH  WSP+AIKSAIVTTAW +DP G  IFA+G     A PFD+GGG++NP KAV PGL+
Sbjct: 563 SLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGSSRKLADPFDYGGGLINPEKAVKPGLI 622

Query: 619 YDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIPSLTHPVSLTR 678
           YDM   DY+ Y C+  Y++ +IS +  +   CP  +PS+LD+N+PSITIP+L   V+LTR
Sbjct: 623 YDMTTDDYVMYMCSVDYSDISISRVLGKITVCPNPKPSVLDLNLPSITIPNLRGEVTLTR 682

Query: 679 TVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGS 738
           TVTNVG +NS YK  I+ P GI +A+ P  L F++     SFTV +S+ H+V TGY FGS
Sbjct: 683 TVTNVGPVNSVYKVVIDPPTGINVAVTPAELVFDYTTTKRSFTVRVSTTHKVNTGYYFGS 742

Query: 739 LTWLDGVHSVRIPVSVRTNI 758
           LTW D +H+V IPVSVRT I
Sbjct: 743 LTWTDNMHNVAIPVSVRTQI 748

BLAST of Csor.00g283160 vs. ExPASy Swiss-Prot
Match: Q9ZSB1 (Subtilisin-like protease SBT3.10 OS=Arabidopsis thaliana OX=3702 GN=SBT3.10 PE=3 SV=2)

HSP 1 Score: 810.1 bits (2091), Expect = 2.2e-233
Identity = 421/740 (56.89%), Postives = 534/740 (72.16%), Query Frame = 0

Query: 19  VAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKL 78
           V A+S VY+VY+G+K HDN E + ++HH +L S+LGS+E  +DS+VYSY++GFSGFAAKL
Sbjct: 23  VVAESKVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAKL 82

Query: 79  TMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLD 138
           T +QAQ ISELP VV+VIPN L++M TTR+WDYL +SP   +SLL+K+ MG   I+GV+D
Sbjct: 83  TESQAQQISELPEVVQVIPNTLYEMTTTRTWDYLGVSPGNSDSLLQKANMGYNVIVGVID 142

Query: 139 TGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPL 198
           TG+WPESE+F D+G GP+PSRWKG CESGELF+ +  C+RKLIGA+YF+    A +G  L
Sbjct: 143 TGVWPESEMFNDKGYGPIPSRWKGGCESGELFNGSIHCNRKLIGAKYFIDANNAQFG-VL 202

Query: 199 NNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQ 258
           N +E  DY SPRD +GHGTHV+S   G F+PNVSY GL  GT RGGAP   IA+YK CW 
Sbjct: 203 NKTENPDYLSPRDFNGHGTHVASTIGGSFLPNVSYLGLGRGTARGGAPGVHIAVYKACWV 262

Query: 259 VNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVV 318
             G  C+  D+LKA+D+AI+DGVD+LSLSL  S P + + D R   ++GAFHAVAKGI V
Sbjct: 263 QRG--CSGADVLKAMDEAIHDGVDILSLSLQTSVPLFPETDARELTSVGAFHAVAKGIPV 322

Query: 319 VGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHN 378
           V AA N+GP A ++SN+ PW+LTVAA++ DRSF  AITLGNN T +GQ +F G    F  
Sbjct: 323 VAAASNAGPTAQTLSNVAPWVLTVAATTQDRSFPTAITLGNNITILGQAIFGGSELGFVG 382

Query: 379 LVYPEVSDLDDERACESLSLNDTWA-AGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIV 438
           L YPE S L  +  CE LS N   A  G VVLCFA+   +  ++   ++V   GGLGLI+
Sbjct: 383 LTYPE-SPLSGD--CEKLSANPKSAMEGKVVLCFAA---STPSNAAITAVINAGGLGLIM 442

Query: 439 AKNPTKAVEPFVNNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYF 498
           A+NPT  + P + NFP V +  ++G  IL YIRSTR+P V I AS T  GQ +ST VA F
Sbjct: 443 ARNPTHLLRP-LRNFPYVSVDFELGTDILFYIRSTRSPIVNIQASRTLFGQSVSTKVATF 502

Query: 499 SSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLK 558
           SSRGPNSV+PAILKPDIAAPGV ILAA+ P+   D   +A +SGTSMATP VS +V LLK
Sbjct: 503 SSRGPNSVSPAILKPDIAAPGVNILAAISPNSIND-GGFAMMSGTSMATPVVSGVVVLLK 562

Query: 559 TLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLV 618
           +LH  WSP+AIKSAIVTTAW +DP G  IFA+G     A PFD+GGG++NP KAV PGL+
Sbjct: 563 SLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGSSRKLADPFDYGGGLINPEKAVKPGLI 622

Query: 619 YDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIPSLTHPVSLTR 678
           YDM   DY+ Y C+  Y++ +IS +  +   CP  +PS+LD+N+PSITIP+L   V+LTR
Sbjct: 623 YDMTTDDYVMYMCSVDYSDISISRVLGKITVCPNPKPSVLDLNLPSITIPNLRGEVTLTR 682

Query: 679 TVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGS 738
           TVTNVG +NS YK  I+ P G+ +A+ P  L F+      SFTV +S+ H+V TGY FGS
Sbjct: 683 TVTNVGPVNSVYKVVIDPPTGVNVAVTPTELVFDSTTTKRSFTVRVSTTHKVNTGYYFGS 742

Query: 739 LTWLDGVHSVRIPVSVRTNI 758
           LTW D +H+V IPVSVRT I
Sbjct: 743 LTWTDTLHNVAIPVSVRTQI 751

BLAST of Csor.00g283160 vs. ExPASy Swiss-Prot
Match: Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 802.7 bits (2072), Expect = 3.5e-231
Identity = 401/745 (53.83%), Postives = 529/745 (71.01%), Query Frame = 0

Query: 20  AAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLT 79
           +AK  V+IVY+G+K HD+ E + ++HH +L S+LGS+E + DS+VYSY++GFSGFAAKLT
Sbjct: 29  SAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLT 88

Query: 80  MAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDT 139
            +QA+ I++LP VV VIP+  +K+ TTR+WDYL LS   P SLL ++ MG   IIGV+DT
Sbjct: 89  ESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAANPKSLLHETNMGEQIIIGVIDT 148

Query: 140 GIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLN 199
           G+WPESEVF D G GPVPS WKG CE+GE F+ +  C++KLIGA+YF+ G   A     N
Sbjct: 149 GVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSN-CNKKLIGAKYFINGF-LAENESFN 208

Query: 200 NSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQV 259
           ++   D+ SPRD  GHGTHVS++A G FVPN+SY GLA GTVRGGAP + IAMYK CW +
Sbjct: 209 STNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLAGGTVRGGAPRAHIAMYKACWYL 268

Query: 260 NGG---VCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGI 319
           +      C+  DILKA+D+A++DGVDVLS+SLG S P Y + D+R+GI  GAFHAV KGI
Sbjct: 269 DDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSSVPLYGETDIRDGITTGAFHAVLKGI 328

Query: 320 VVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQGMFSGKLTKF 379
            VV + GNSGP + +V+N  PW++TVAA+++DRSF   +TLGNN   +GQ M++G    F
Sbjct: 329 TVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFATPLTLGNNKVILGQAMYTGPGLGF 388

Query: 380 HNLVYPEVSDLDDER---ACESLSLN-DTWAAGNVVLCFASDDYNDDTHNTSSSVKKVGG 439
            +LVYPE     +E     CE L  N +    G VVLCF +  Y     + +  VK+ GG
Sbjct: 389 TSLVYPENPGNSNESFSGTCEELLFNSNRTMEGKVVLCFTTSPYGGAVLSAARYVKRAGG 448

Query: 440 LGLIVAKNPTKAVEPFVNNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVGQPLST 499
           LG+I+A++P  A++P +++FPCV +  ++G  IL Y RS+ +P VKI  S T VGQP+ T
Sbjct: 449 LGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILLYTRSSGSPVVKIQPSKTLVGQPVGT 508

Query: 500 TVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATPHVSAI 559
            VA FSSRGPNS+APAILKPDIAAPGV+ILAA   +   D+  +  +SGTSMA P +S +
Sbjct: 509 KVATFSSRGPNSIAPAILKPDIAAPGVSILAATTNTTFSDQ-GFIMLSGTSMAAPAISGV 568

Query: 560 VALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVNPNKAV 619
            ALLK LH  WSPAAI+SAIVTTAW +DP+G  IFAEG P   A PFD+GGG+VNP K+ 
Sbjct: 569 AALLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIFAEGSPPKLADPFDYGGGLVNPEKSA 628

Query: 620 DPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIPSLTHP 679
           +PGLVYDMG+ DY+ Y C+ GYN ++IS +  ++  C   +PS+LD N+PSITIP+L   
Sbjct: 629 NPGLVYDMGLEDYVLYMCSVGYNETSISQLIGKTTVCSNPKPSVLDFNLPSITIPNLKDE 688

Query: 680 VSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNHRVTTG 739
           V++TRTVTNVG +NS Y+  +E P G  + + P  L FN   K + F V +S+ H+  TG
Sbjct: 689 VTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPETLVFNSTTKKVYFKVKVSTTHKTNTG 748

Query: 740 YCFGSLTWLDGVHSVRIPVSVRTNI 758
           Y FGSLTW D +H+V IP+SVRT I
Sbjct: 749 YYFGSLTWSDSLHNVTIPLSVRTQI 770

BLAST of Csor.00g283160 vs. ExPASy Swiss-Prot
Match: Q9SZY3 (Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana OX=3702 GN=SBT3.8 PE=3 SV=1)

HSP 1 Score: 800.8 bits (2067), Expect = 1.3e-230
Identity = 407/756 (53.84%), Postives = 531/756 (70.24%), Query Frame = 0

Query: 9   LDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYK 68
           L+ L T      A+S V+IVY+G+K HD+ E + ++HH +L S+LGS+E +  S+V+SY+
Sbjct: 14  LNGLSTFVAHAGAESKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDAHSSMVHSYR 73

Query: 69  YGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSRM 128
           +GFSGFAAKLT +QA+ +++LP VV V P+  +++ TTR+WDYL LS   P +LL  + M
Sbjct: 74  HGFSGFAAKLTKSQAKKLADLPEVVHVTPDSFYQLDTTRTWDYLGLSVANPKNLLNDTNM 133

Query: 129 GSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVK 188
           G   IIG++D+G+WPESEVF D G+GPVPS WKG C SGE F+ ++ C++KLIGA+YF+ 
Sbjct: 134 GEEVIIGIVDSGVWPESEVFNDNGIGPVPSHWKGGCVSGENFTSSQ-CNKKLIGAKYFIN 193

Query: 189 GLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHS 248
           G  A +    N++E  D+ SPRDRSGHGTHV+++A G +VP++SY GLA GTVRGGAP +
Sbjct: 194 GFLATH-ESFNSTESLDFISPRDRSGHGTHVATIAGGSYVPSISYKGLAGGTVRGGAPRA 253

Query: 249 RIAMYKVCWQVNG---GVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIA 308
           RIAMYK CW ++      C+  DILKA+D+A++DGVDVLSLS+G  FP + + D+R  IA
Sbjct: 254 RIAMYKACWYLDRFDINTCSSADILKAMDEAMHDGVDVLSLSIGYRFPYFPETDVRAVIA 313

Query: 309 IGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMG 368
            GAFHAV KGI VV + GNSGPAA +V N  PW+LTVAA+++DRSF   ITLGNN   +G
Sbjct: 314 TGAFHAVLKGITVVCSGGNSGPAAQTVGNTAPWILTVAATTLDRSFPTPITLGNNKLILG 373

Query: 369 QGMFSGKLTKFHNLVYPEVSDLDDER---ACESLSLNDTWA-AGNVVLCFASDDYNDDTH 428
           Q M++G    F +LVYPE     +E     CE L  N     AG VVLCF +        
Sbjct: 374 QAMYTGPELGFTSLVYPENPGNSNESFSGDCELLFFNSNHTMAGKVVLCFTTSTRYITVS 433

Query: 429 NTSSSVKKVGGLGLIVAKNPTKAVEPFVNNFPCVQISLDIGMQILNYIRSTRNPQVKIGA 488
           +  S VK+ GGLG+IVA+NP   + P  ++FPCV +  ++G  IL YIRST  P VKI  
Sbjct: 434 SAVSYVKEAGGLGVIVARNPGDNLSPCEDDFPCVAVDYELGTDILLYIRSTGLPVVKIQP 493

Query: 489 STTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISG 548
           S T VGQP+ T VA FSSRGPNS+ PAILKPDIAAPGV+ILAA   +   +   + F+SG
Sbjct: 494 SKTLVGQPVGTKVADFSSRGPNSIEPAILKPDIAAPGVSILAATTTNKTFNDRGFIFLSG 553

Query: 549 TSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDF 608
           TSMA P +S +VALLK LH  WSPAAI+SAIVTTAW +DP+G  IFAEG P   A PFD+
Sbjct: 554 TSMAAPTISGVVALLKALHRDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKLADPFDY 613

Query: 609 GGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINV 668
           GGG+VNP KA  PGLVYD+G+ DY+ Y C+ GYN ++IS +  +   C   +PS+LD N+
Sbjct: 614 GGGLVNPEKAAKPGLVYDLGLEDYVLYMCSVGYNETSISQLVGKGTVCSNPKPSVLDFNL 673

Query: 669 PSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTV 728
           PSITIP+L   V+LTRT+TNVG + S YK  IE P GI + + P  L FN   K +SF V
Sbjct: 674 PSITIPNLKDEVTLTRTLTNVGQLESVYKVVIEPPIGIQVTVTPETLLFNSTTKRVSFKV 733

Query: 729 TISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 758
            +S+ H++ TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 734 KVSTTHKINTGYFFGSLTWSDSLHNVTIPLSVRTQI 767

BLAST of Csor.00g283160 vs. NCBI nr
Match: KAG6576624.1 (Subtilisin-like protease 3.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1520 bits (3935), Expect = 0.0
Identity = 757/757 (100.00%), Postives = 757/757 (100.00%), Query Frame = 0

Query: 1   MGNAATPALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSV 60
           MGNAATPALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSV
Sbjct: 1   MGNAATPALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSV 60

Query: 61  DSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPN 120
           DSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPN
Sbjct: 61  DSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPN 120

Query: 121 SLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKL 180
           SLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKL
Sbjct: 121 SLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKL 180

Query: 181 IGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGT 240
           IGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGT
Sbjct: 181 IGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGT 240

Query: 241 VRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDM 300
           VRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDM
Sbjct: 241 VRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDM 300

Query: 301 RNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNN 360
           RNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNN
Sbjct: 301 RNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNN 360

Query: 361 WTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDT 420
           WTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDT
Sbjct: 361 WTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDT 420

Query: 421 HNTSSSVKKVGGLGLIVAKNPTKAVEPFVNNFPCVQISLDIGMQILNYIRSTRNPQVKIG 480
           HNTSSSVKKVGGLGLIVAKNPTKAVEPFVNNFPCVQISLDIGMQILNYIRSTRNPQVKIG
Sbjct: 421 HNTSSSVKKVGGLGLIVAKNPTKAVEPFVNNFPCVQISLDIGMQILNYIRSTRNPQVKIG 480

Query: 481 ASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFIS 540
           ASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFIS
Sbjct: 481 ASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFIS 540

Query: 541 GTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFD 600
           GTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFD
Sbjct: 541 GTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFD 600

Query: 601 FGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDIN 660
           FGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDIN
Sbjct: 601 FGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDIN 660

Query: 661 VPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFT 720
           VPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFT
Sbjct: 661 VPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFT 720

Query: 721 VTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 757
           VTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI
Sbjct: 721 VTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 757

BLAST of Csor.00g283160 vs. NCBI nr
Match: KAG7014677.1 (Subtilisin-like protease SBT3.3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1503 bits (3892), Expect = 0.0
Identity = 748/750 (99.73%), Postives = 750/750 (100.00%), Query Frame = 0

Query: 8   ALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSY 67
           ALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSY
Sbjct: 24  ALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSY 83

Query: 68  KYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSR 127
           KYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSR
Sbjct: 84  KYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSR 143

Query: 128 MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV 187
           MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV
Sbjct: 144 MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV 203

Query: 188 KGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH 247
           KGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH
Sbjct: 204 KGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH 263

Query: 248 SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG 307
           SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG
Sbjct: 264 SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG 323

Query: 308 AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG 367
           AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG
Sbjct: 324 AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG 383

Query: 368 MFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDTHNTSSSV 427
           MFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDTHNTSSSV
Sbjct: 384 MFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDTHNTSSSV 443

Query: 428 KKVGGLGLIVAKNPTKAVEPFVNNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG 487
           KKVGGLGLIVAKNPTKAVEPF+NNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG
Sbjct: 444 KKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG 503

Query: 488 QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATP 547
           QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATP
Sbjct: 504 QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATP 563

Query: 548 HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVN 607
           HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVN
Sbjct: 564 HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVN 623

Query: 608 PNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIP 667
           PNKAVDPGLVYDMGMADYIQYFCAKGYN+SAISGITKRSISCPKRRPSILDINVPSITIP
Sbjct: 624 PNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITKRSISCPKRRPSILDINVPSITIP 683

Query: 668 SLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNH 727
           SLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNH
Sbjct: 684 SLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNH 743

Query: 728 RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 757
           RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI
Sbjct: 744 RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Csor.00g283160 vs. NCBI nr
Match: XP_022922598.1 (subtilisin-like protease SBT3.9 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1502 bits (3888), Expect = 0.0
Identity = 747/750 (99.60%), Postives = 749/750 (99.87%), Query Frame = 0

Query: 8   ALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSY 67
           ALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSY
Sbjct: 24  ALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSY 83

Query: 68  KYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSR 127
           KYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSR
Sbjct: 84  KYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSR 143

Query: 128 MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV 187
           MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV
Sbjct: 144 MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV 203

Query: 188 KGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH 247
           KGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH
Sbjct: 204 KGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH 263

Query: 248 SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG 307
           SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG
Sbjct: 264 SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG 323

Query: 308 AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG 367
           AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVD SFLVAITLGNNWTTMGQG
Sbjct: 324 AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDCSFLVAITLGNNWTTMGQG 383

Query: 368 MFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDTHNTSSSV 427
           MFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDTHNTSSSV
Sbjct: 384 MFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDTHNTSSSV 443

Query: 428 KKVGGLGLIVAKNPTKAVEPFVNNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG 487
           KKVGGLGLIVAKNPTKAVEPF+NNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG
Sbjct: 444 KKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG 503

Query: 488 QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATP 547
           QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATP
Sbjct: 504 QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATP 563

Query: 548 HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVN 607
           HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVN
Sbjct: 564 HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVN 623

Query: 608 PNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIP 667
           PNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIP
Sbjct: 624 PNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIP 683

Query: 668 SLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNH 727
           SLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIA+KPRILKFNHKMKSISFTVTISSNH
Sbjct: 684 SLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAVKPRILKFNHKMKSISFTVTISSNH 743

Query: 728 RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 757
           RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI
Sbjct: 744 RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Csor.00g283160 vs. NCBI nr
Match: XP_023551712.1 (subtilisin-like protease SBT3.9 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1496 bits (3874), Expect = 0.0
Identity = 743/750 (99.07%), Postives = 748/750 (99.73%), Query Frame = 0

Query: 8   ALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSY 67
           ALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSY
Sbjct: 24  ALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSY 83

Query: 68  KYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSR 127
           KYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHK+QTTRSWDYLQLSPRFPNSLLRKSR
Sbjct: 84  KYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKIQTTRSWDYLQLSPRFPNSLLRKSR 143

Query: 128 MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV 187
           MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV
Sbjct: 144 MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV 203

Query: 188 KGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH 247
           KGLEAAYGHPLNNS+FQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH
Sbjct: 204 KGLEAAYGHPLNNSKFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH 263

Query: 248 SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG 307
           SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG
Sbjct: 264 SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG 323

Query: 308 AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG 367
           AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG
Sbjct: 324 AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG 383

Query: 368 MFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDTHNTSSSV 427
           MFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAAGNVVLCFASDD+NDDTHNTSSSV
Sbjct: 384 MFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAAGNVVLCFASDDFNDDTHNTSSSV 443

Query: 428 KKVGGLGLIVAKNPTKAVEPFVNNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG 487
           KKVGGLGLIVAKNPTKAVEPF+NNFPCVQISLD GMQILNYIRSTRNPQVKIGASTTRVG
Sbjct: 444 KKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDTGMQILNYIRSTRNPQVKIGASTTRVG 503

Query: 488 QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATP 547
           QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATP
Sbjct: 504 QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATP 563

Query: 548 HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVN 607
           HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVN
Sbjct: 564 HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVN 623

Query: 608 PNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIP 667
           PNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIP
Sbjct: 624 PNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIP 683

Query: 668 SLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNH 727
           SLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIA+KPRILKFNHKMKSI FTVTISSNH
Sbjct: 684 SLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAVKPRILKFNHKMKSIPFTVTISSNH 743

Query: 728 RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 757
           RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI
Sbjct: 744 RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Csor.00g283160 vs. NCBI nr
Match: XP_022984356.1 (subtilisin-like protease SBT3.9 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1471 bits (3809), Expect = 0.0
Identity = 734/750 (97.87%), Postives = 743/750 (99.07%), Query Frame = 0

Query: 8   ALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSY 67
           ALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSY
Sbjct: 24  ALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSY 83

Query: 68  KYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSR 127
           KYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHK+QTTRSWDYLQLSPRFPNSLLRKSR
Sbjct: 84  KYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKIQTTRSWDYLQLSPRFPNSLLRKSR 143

Query: 128 MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV 187
           MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV
Sbjct: 144 MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV 203

Query: 188 KGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH 247
           KGLEAAYGHPLNNSEFQDY SPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH
Sbjct: 204 KGLEAAYGHPLNNSEFQDYLSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH 263

Query: 248 SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG 307
           SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG
Sbjct: 264 SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG 323

Query: 308 AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG 367
           AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG
Sbjct: 324 AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG 383

Query: 368 MFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDTHNTSSSV 427
           MFSGKLTKF NLVYPEVSDLDDERACESLSLNDTWA+GNVVLCFASDDYND+THNTS SV
Sbjct: 384 MFSGKLTKFINLVYPEVSDLDDERACESLSLNDTWASGNVVLCFASDDYNDNTHNTSWSV 443

Query: 428 KKVGGLGLIVAKNPTKAVEPFVNNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG 487
           KKVGGLGLIVAKNPTKAVEPF++NFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG
Sbjct: 444 KKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG 503

Query: 488 QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATP 547
           QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPK+RNSYAFISGTSMATP
Sbjct: 504 QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKNRNSYAFISGTSMATP 563

Query: 548 HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVN 607
           HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEG+PMMSARPFDFGGGIVN
Sbjct: 564 HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGRPMMSARPFDFGGGIVN 623

Query: 608 PNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIP 667
           PNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGI+KRSISCPKRRPSILDINVPSITIP
Sbjct: 624 PNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGISKRSISCPKRRPSILDINVPSITIP 683

Query: 668 SLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNH 727
           SLTH VSLTRTVTNVGAINSSYKAAIE PPGITIA+KPRILKFNHK KSISFTVTISSN 
Sbjct: 684 SLTHSVSLTRTVTNVGAINSSYKAAIEVPPGITIAVKPRILKFNHKTKSISFTVTISSNQ 743

Query: 728 RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 757
           RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI
Sbjct: 744 RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Csor.00g283160 vs. ExPASy TrEMBL
Match: A0A6J1E757 (subtilisin-like protease SBT3.9 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430558 PE=3 SV=1)

HSP 1 Score: 1502 bits (3888), Expect = 0.0
Identity = 747/750 (99.60%), Postives = 749/750 (99.87%), Query Frame = 0

Query: 8   ALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSY 67
           ALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSY
Sbjct: 24  ALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSY 83

Query: 68  KYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSR 127
           KYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSR
Sbjct: 84  KYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSR 143

Query: 128 MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV 187
           MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV
Sbjct: 144 MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV 203

Query: 188 KGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH 247
           KGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH
Sbjct: 204 KGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH 263

Query: 248 SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG 307
           SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG
Sbjct: 264 SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG 323

Query: 308 AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG 367
           AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVD SFLVAITLGNNWTTMGQG
Sbjct: 324 AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDCSFLVAITLGNNWTTMGQG 383

Query: 368 MFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDTHNTSSSV 427
           MFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDTHNTSSSV
Sbjct: 384 MFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDTHNTSSSV 443

Query: 428 KKVGGLGLIVAKNPTKAVEPFVNNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG 487
           KKVGGLGLIVAKNPTKAVEPF+NNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG
Sbjct: 444 KKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG 503

Query: 488 QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATP 547
           QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATP
Sbjct: 504 QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATP 563

Query: 548 HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVN 607
           HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVN
Sbjct: 564 HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVN 623

Query: 608 PNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIP 667
           PNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIP
Sbjct: 624 PNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIP 683

Query: 668 SLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNH 727
           SLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIA+KPRILKFNHKMKSISFTVTISSNH
Sbjct: 684 SLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAVKPRILKFNHKMKSISFTVTISSNH 743

Query: 728 RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 757
           RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI
Sbjct: 744 RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Csor.00g283160 vs. ExPASy TrEMBL
Match: A0A6J1J508 (subtilisin-like protease SBT3.9 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482683 PE=3 SV=1)

HSP 1 Score: 1471 bits (3809), Expect = 0.0
Identity = 734/750 (97.87%), Postives = 743/750 (99.07%), Query Frame = 0

Query: 8   ALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSY 67
           ALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSY
Sbjct: 24  ALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSY 83

Query: 68  KYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSR 127
           KYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHK+QTTRSWDYLQLSPRFPNSLLRKSR
Sbjct: 84  KYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKIQTTRSWDYLQLSPRFPNSLLRKSR 143

Query: 128 MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV 187
           MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV
Sbjct: 144 MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV 203

Query: 188 KGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH 247
           KGLEAAYGHPLNNSEFQDY SPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH
Sbjct: 204 KGLEAAYGHPLNNSEFQDYLSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH 263

Query: 248 SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG 307
           SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG
Sbjct: 264 SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG 323

Query: 308 AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG 367
           AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG
Sbjct: 324 AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG 383

Query: 368 MFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDTHNTSSSV 427
           MFSGKLTKF NLVYPEVSDLDDERACESLSLNDTWA+GNVVLCFASDDYND+THNTS SV
Sbjct: 384 MFSGKLTKFINLVYPEVSDLDDERACESLSLNDTWASGNVVLCFASDDYNDNTHNTSWSV 443

Query: 428 KKVGGLGLIVAKNPTKAVEPFVNNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG 487
           KKVGGLGLIVAKNPTKAVEPF++NFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG
Sbjct: 444 KKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG 503

Query: 488 QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATP 547
           QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPK+RNSYAFISGTSMATP
Sbjct: 504 QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKNRNSYAFISGTSMATP 563

Query: 548 HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVN 607
           HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEG+PMMSARPFDFGGGIVN
Sbjct: 564 HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGRPMMSARPFDFGGGIVN 623

Query: 608 PNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIP 667
           PNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGI+KRSISCPKRRPSILDINVPSITIP
Sbjct: 624 PNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGISKRSISCPKRRPSILDINVPSITIP 683

Query: 668 SLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNH 727
           SLTH VSLTRTVTNVGAINSSYKAAIE PPGITIA+KPRILKFNHK KSISFTVTISSN 
Sbjct: 684 SLTHSVSLTRTVTNVGAINSSYKAAIEVPPGITIAVKPRILKFNHKTKSISFTVTISSNQ 743

Query: 728 RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 757
           RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI
Sbjct: 744 RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Csor.00g283160 vs. ExPASy TrEMBL
Match: A0A6J1CIU2 (subtilisin-like protease SBT3.9 OS=Momordica charantia OX=3673 GN=LOC111011560 PE=3 SV=1)

HSP 1 Score: 1323 bits (3425), Expect = 0.0
Identity = 648/751 (86.28%), Postives = 699/751 (93.08%), Query Frame = 0

Query: 9   LDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYK 68
           L  LCTL + V AKS+VY+VYMG++PHDNEELLVK HHGVLASVLGSQE   DS+VY+YK
Sbjct: 25  LSDLCTLLVPVDAKSHVYVVYMGKRPHDNEELLVKTHHGVLASVLGSQEAPADSMVYNYK 84

Query: 69  YGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSRM 128
           YGFSGFAAKLTMAQAQ ISELP+V++VIPNRLHKMQTTRSWDYLQLSPR  NSLL+KSRM
Sbjct: 85  YGFSGFAAKLTMAQAQKISELPTVIKVIPNRLHKMQTTRSWDYLQLSPRLQNSLLQKSRM 144

Query: 129 GSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVK 188
           G+GAIIG+LDTGIWPESEVF D GLGPVPSRWKGICESGELFSP KACSRKLIGARYF+K
Sbjct: 145 GNGAIIGLLDTGIWPESEVFSDNGLGPVPSRWKGICESGELFSPGKACSRKLIGARYFIK 204

Query: 189 GLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHS 248
           GLEAAYGHP NNS FQDY SPRD +GHGTHVSSVASG FV NVSYHGLAVGTVRGGAP S
Sbjct: 205 GLEAAYGHPYNNSGFQDYLSPRDYAGHGTHVSSVASGSFVANVSYHGLAVGTVRGGAPQS 264

Query: 249 RIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIGA 308
           R+AMYKVCWQ+NGGVC+D DILKAIDQAI+DGVDVLSLSLGP+FPSYSDVDM NG+AIG 
Sbjct: 265 RLAMYKVCWQLNGGVCSDADILKAIDQAIHDGVDVLSLSLGPTFPSYSDVDMHNGVAIGT 324

Query: 309 FHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQGM 368
           FHA+ KGIVVVGAAGN GPAAYSV+NIEPWLLTVAASSVDRSFLVAITLGNNWTT+GQGM
Sbjct: 325 FHAIVKGIVVVGAAGNYGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNNWTTVGQGM 384

Query: 369 FSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDTHNTSSSVK 428
           FSGKL +FHNLVYPEVSDL+DERACESLSLNDTWAAGNVVLCFASDD+ND+TH+TS SVK
Sbjct: 385 FSGKLRRFHNLVYPEVSDLEDERACESLSLNDTWAAGNVVLCFASDDFNDNTHHTSRSVK 444

Query: 429 KVGGLGLIVAKNPTKAVEPFVNNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVGQ 488
           KVG LGLIVAKNPTK VEPF+N+FPCVQ+SL+IGM ILNYIRSTRNPQV IG S TR+GQ
Sbjct: 445 KVGALGLIVAKNPTKDVEPFINHFPCVQVSLEIGMHILNYIRSTRNPQVNIGPSRTRIGQ 504

Query: 489 PLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATPH 548
            LSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKD+NSYAFISGTSMATPH
Sbjct: 505 SLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDKNSYAFISGTSMATPH 564

Query: 549 VSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVNP 608
           VSAIVALLK+LHSHWSPAAIKSAIVTTAW+SDPYG  IFAEG+P+ SA PFDFGGGIVNP
Sbjct: 565 VSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGETIFAEGRPIKSAGPFDFGGGIVNP 624

Query: 609 NKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIPS 668
           NKAVDPGLVYDMGMADYI+YFCA GYN+SAISGITK+ ISCPKRRPSILD NVPSITIP 
Sbjct: 625 NKAVDPGLVYDMGMADYIRYFCAMGYNDSAISGITKKPISCPKRRPSILDTNVPSITIPG 684

Query: 669 LTHPVSLTRTVTNVGAINSSYKAAIEAPPG--ITIAIKPRILKFNHKMKSISFTVTISSN 728
           L H V++TRTVTNVGA+NS+YKAAIE PPG  I IA+KPR+LKFNHK K ISFTVT+SSN
Sbjct: 685 LKHSVTVTRTVTNVGAVNSTYKAAIETPPGPGIAIAVKPRMLKFNHKRKIISFTVTVSSN 744

Query: 729 HRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 757
           HRVTTGYCFGSLTWLDGVHSVRIP+SVRT+I
Sbjct: 745 HRVTTGYCFGSLTWLDGVHSVRIPISVRTDI 775

BLAST of Csor.00g283160 vs. ExPASy TrEMBL
Match: A0A6J1E3V3 (subtilisin-like protease SBT3.3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430558 PE=3 SV=1)

HSP 1 Score: 1315 bits (3403), Expect = 0.0
Identity = 652/655 (99.54%), Postives = 654/655 (99.85%), Query Frame = 0

Query: 103 MQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKG 162
           MQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKG
Sbjct: 1   MQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKG 60

Query: 163 ICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSV 222
           ICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSV
Sbjct: 61  ICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSV 120

Query: 223 ASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVD 282
           ASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVD
Sbjct: 121 ASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVD 180

Query: 283 VLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTV 342
           VLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTV
Sbjct: 181 VLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTV 240

Query: 343 AASSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTW 402
           AASSVD SFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTW
Sbjct: 241 AASSVDCSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTW 300

Query: 403 AAGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFVNNFPCVQISLDIG 462
           AAGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPF+NNFPCVQISLDIG
Sbjct: 301 AAGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIG 360

Query: 463 MQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAIL 522
           MQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAIL
Sbjct: 361 MQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAIL 420

Query: 523 AAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPY 582
           AAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPY
Sbjct: 421 AAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPY 480

Query: 583 GAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGI 642
           GAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGI
Sbjct: 481 GAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGI 540

Query: 643 TKRSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIA 702
           TKRSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIA
Sbjct: 541 TKRSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIA 600

Query: 703 IKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 757
           +KPRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI
Sbjct: 601 VKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 655

BLAST of Csor.00g283160 vs. ExPASy TrEMBL
Match: A0A6J1J1Z1 (subtilisin-like protease SBT3.3 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482683 PE=3 SV=1)

HSP 1 Score: 1235 bits (3196), Expect = 0.0
Identity = 615/630 (97.62%), Postives = 623/630 (98.89%), Query Frame = 0

Query: 128 MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV 187
           MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV
Sbjct: 1   MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV 60

Query: 188 KGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH 247
           KGLEAAYGHPLNNSEFQDY SPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH
Sbjct: 61  KGLEAAYGHPLNNSEFQDYLSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH 120

Query: 248 SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG 307
           SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG
Sbjct: 121 SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG 180

Query: 308 AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG 367
           AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG
Sbjct: 181 AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG 240

Query: 368 MFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDTHNTSSSV 427
           MFSGKLTKF NLVYPEVSDLDDERACESLSLNDTWA+GNVVLCFASDDYND+THNTS SV
Sbjct: 241 MFSGKLTKFINLVYPEVSDLDDERACESLSLNDTWASGNVVLCFASDDYNDNTHNTSWSV 300

Query: 428 KKVGGLGLIVAKNPTKAVEPFVNNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG 487
           KKVGGLGLIVAKNPTKAVEPF++NFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG
Sbjct: 301 KKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG 360

Query: 488 QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATP 547
           QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPK+RNSYAFISGTSMATP
Sbjct: 361 QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKNRNSYAFISGTSMATP 420

Query: 548 HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVN 607
           HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEG+PMMSARPFDFGGGIVN
Sbjct: 421 HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGRPMMSARPFDFGGGIVN 480

Query: 608 PNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIP 667
           PNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGI+KRSISCPKRRPSILDINVPSITIP
Sbjct: 481 PNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGISKRSISCPKRRPSILDINVPSITIP 540

Query: 668 SLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNH 727
           SLTH VSLTRTVTNVGAINSSYKAAIE PPGITIA+KPRILKFNHK KSISFTVTISSN 
Sbjct: 541 SLTHSVSLTRTVTNVGAINSSYKAAIEVPPGITIAVKPRILKFNHKTKSISFTVTISSNQ 600

Query: 728 RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 757
           RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI
Sbjct: 601 RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 630

BLAST of Csor.00g283160 vs. TAIR 10
Match: AT1G32960.1 (Subtilase family protein )

HSP 1 Score: 817.4 bits (2110), Expect = 9.6e-237
Identity = 414/747 (55.42%), Postives = 535/747 (71.62%), Query Frame = 0

Query: 18  RVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAK 77
           R   +S V+IVY+G+K H + E + ++HH +LAS+LGS++ + DS+VYSY++GFSGFAAK
Sbjct: 25  RSETESKVHIVYLGEKKHHDPEFVTESHHQMLASLLGSKKDADDSMVYSYRHGFSGFAAK 84

Query: 78  LTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVL 137
           LT +QA+ I++LP VV VIP+  H++ TTR+W+YL LS   P +LL  + MG   IIGV+
Sbjct: 85  LTKSQAKKIADLPEVVHVIPDGFHELATTRTWEYLGLSSANPKNLLNDTNMGDQVIIGVI 144

Query: 138 DTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVKGLEAAYGHP 197
           DTG+WPESE F D G+GP+P +WKG CESGE F  +  C+RKLIGA+YF+ G   A    
Sbjct: 145 DTGVWPESESFNDNGVGPIPRKWKGGCESGENFR-STDCNRKLIGAKYFINGF-LAENKG 204

Query: 198 LNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCW 257
            N +E +DY S RD  GHGTHV+S+A G FVPNVSY GLA GT+RGGAP +RIAMYK CW
Sbjct: 205 FNTTESRDYISARDFDGHGTHVASIAGGSFVPNVSYKGLAGGTLRGGAPRARIAMYKACW 264

Query: 258 ---QVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAK 317
              ++ G  C+D DI+KAID+AI+DGVDVLS+SL    P  S+ D+R+  A G FHAVAK
Sbjct: 265 FHEELKGVTCSDSDIMKAIDEAIHDGVDVLSISLVGQIPLNSETDIRDEFATGLFHAVAK 324

Query: 318 GIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQGMFSGKLT 377
           GIVVV A GN GPAA +V NI PW+LTVAA+++DRSF   ITLGNN   +GQ  ++G   
Sbjct: 325 GIVVVCAGGNDGPAAQTVVNIAPWILTVAATTLDRSFPTPITLGNNKVILGQATYTGPEL 384

Query: 378 KFHNLVYPEVSDLDDER---ACESLSLNDTWA-AGNVVLCFASDDYNDDTHNTSSSVKKV 437
              +LVYPE +  ++E     CESL+LN  +  A  VVLCF +   N      +S VK  
Sbjct: 385 GLTSLVYPENARNNNETFSGVCESLNLNPNYTMAMKVVLCFTASRTNAAISRAASFVKAA 444

Query: 438 GGLGLIVAKNPTKAVEPFVNNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVGQPL 497
           GGLGLI+++NP   + P  ++FPCV +  ++G  IL+YIRSTR+P VKI  S T  GQP+
Sbjct: 445 GGLGLIISRNPVYTLSPCNDDFPCVAVDYELGTDILSYIRSTRSPVVKIQRSRTLSGQPV 504

Query: 498 STTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATPHVS 557
            T V  FSSRGPNS++PAILKPDIAAPGV ILAA  P+D  +   +A +SGTSMATP +S
Sbjct: 505 GTKVVNFSSRGPNSMSPAILKPDIAAPGVRILAATSPNDTLNVGGFAMLSGTSMATPVIS 564

Query: 558 AIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVNPNK 617
            ++ALLK LH  WSPAA +SAIVTTAW +DP+G  IFAEG     + PFD+GGGIVNP K
Sbjct: 565 GVIALLKALHPEWSPAAFRSAIVTTAWRTDPFGEQIFAEGSSRKVSDPFDYGGGIVNPEK 624

Query: 618 AVDPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIPSLT 677
           A +PGL+YDMG  DYI Y C+ GYN+S+IS +  +   C   +PS+LD+N+PSITIP+L 
Sbjct: 625 AAEPGLIYDMGPQDYILYLCSAGYNDSSISQLVGQITVCSNPKPSVLDVNLPSITIPNLK 684

Query: 678 HPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNHRVT 737
             V+LTRTVTNVG ++S YK ++E P G+ + + P  L FN K  S+SFTV +S+ H++ 
Sbjct: 685 DEVTLTRTVTNVGLVDSVYKVSVEPPLGVRVVVTPETLVFNSKTISVSFTVRVSTTHKIN 744

Query: 738 TGYCFGSLTWLDGVHSVRIPVSVRTNI 758
           TGY FGSLTW D VH+V IP+SVRT I
Sbjct: 745 TGYYFGSLTWTDSVHNVVIPLSVRTQI 769

BLAST of Csor.00g283160 vs. TAIR 10
Match: AT4G10520.1 (Subtilase family protein )

HSP 1 Score: 813.5 bits (2100), Expect = 1.4e-235
Identity = 421/740 (56.89%), Postives = 540/740 (72.97%), Query Frame = 0

Query: 19  VAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKL 78
           V A+S VY+VY+G+K HDN E + ++HH +L S+LGS+E  +DS+VYSY++GFSGFAAKL
Sbjct: 23  VVAESKVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAKL 82

Query: 79  TMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLD 138
           T +QAQ ISELP VV+VIPN L++M TTR+WDYL +SP   +SLL+K+ MG   I+GV+D
Sbjct: 83  TESQAQQISELPEVVQVIPNTLYEMTTTRTWDYLGVSPGNSDSLLQKANMGYNVIVGVID 142

Query: 139 TGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPL 198
           +G+WPESE+F D+G GP+PSRWKG CESGELF+ +  C+RKLIGA+YFV GL A +G  +
Sbjct: 143 SGVWPESEMFNDKGFGPIPSRWKGGCESGELFNASIHCNRKLIGAKYFVDGLVAEFG-VV 202

Query: 199 NNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQ 258
           N ++  +Y SPRD +GHGTHV+S   G F+PNVSY GL  GT RGGAP   IA+YK CW 
Sbjct: 203 NRTQNPEYLSPRDFAGHGTHVASTIGGSFLPNVSYVGLGRGTARGGAPGVHIAVYKACW- 262

Query: 259 VNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVV 318
              G C+  D+LKA+D+AI+DGVD+LSLSLGPS P + + +     ++GAFHAVAKGI V
Sbjct: 263 --SGYCSGADVLKAMDEAIHDGVDILSLSLGPSVPLFPETE---HTSVGAFHAVAKGIPV 322

Query: 319 VGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHN 378
           V AAGN+GP A ++SN+ PW+LTVAA++ DRSF  AITLGNN T +GQ ++ G    F  
Sbjct: 323 VIAAGNAGPTAQTISNVAPWVLTVAATTQDRSFPTAITLGNNITILGQAIYGGPELGFVG 382

Query: 379 LVYPEVSDLDDERACESLSLN-DTWAAGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIV 438
           L YPE S L  +  CE LS N ++   G VVLCFA+   +  ++   ++V   GGLGLI+
Sbjct: 383 LTYPE-SPLSGD--CEKLSANPNSTMEGKVVLCFAA---STPSNAAIAAVINAGGLGLIM 442

Query: 439 AKNPTKAVEPFVNNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYF 498
           AKNPT ++ P    FP V I  ++G  IL YIRSTR+P VKI AS T  GQ +ST VA F
Sbjct: 443 AKNPTHSLTP-TRKFPWVSIDFELGTDILFYIRSTRSPIVKIQASKTLFGQSVSTKVATF 502

Query: 499 SSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLK 558
           SSRGPNSV+PAILKPDIAAPGV ILAA+ P+   +   +A +SGTSMATP VS +V LLK
Sbjct: 503 SSRGPNSVSPAILKPDIAAPGVNILAAISPNSSINDGGFAMMSGTSMATPVVSGVVVLLK 562

Query: 559 TLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLV 618
           +LH  WSP+AIKSAIVTTAW +DP G  IFA+G     A PFD+GGG++NP KAV PGL+
Sbjct: 563 SLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGSSRKLADPFDYGGGLINPEKAVKPGLI 622

Query: 619 YDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIPSLTHPVSLTR 678
           YDM   DY+ Y C+  Y++ +IS +  +   CP  +PS+LD+N+PSITIP+L   V+LTR
Sbjct: 623 YDMTTDDYVMYMCSVDYSDISISRVLGKITVCPNPKPSVLDLNLPSITIPNLRGEVTLTR 682

Query: 679 TVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGS 738
           TVTNVG +NS YK  I+ P GI +A+ P  L F++     SFTV +S+ H+V TGY FGS
Sbjct: 683 TVTNVGPVNSVYKVVIDPPTGINVAVTPAELVFDYTTTKRSFTVRVSTTHKVNTGYYFGS 742

Query: 739 LTWLDGVHSVRIPVSVRTNI 758
           LTW D +H+V IPVSVRT I
Sbjct: 743 LTWTDNMHNVAIPVSVRTQI 748

BLAST of Csor.00g283160 vs. TAIR 10
Match: AT4G10550.1 (Subtilase family protein )

HSP 1 Score: 802.7 bits (2072), Expect = 2.5e-232
Identity = 401/745 (53.83%), Postives = 529/745 (71.01%), Query Frame = 0

Query: 20  AAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLT 79
           +AK  V+IVY+G+K HD+ E + ++HH +L S+LGS+E + DS+VYSY++GFSGFAAKLT
Sbjct: 29  SAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLT 88

Query: 80  MAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDT 139
            +QA+ I++LP VV VIP+  +K+ TTR+WDYL LS   P SLL ++ MG   IIGV+DT
Sbjct: 89  ESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAANPKSLLHETNMGEQIIIGVIDT 148

Query: 140 GIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLN 199
           G+WPESEVF D G GPVPS WKG CE+GE F+ +  C++KLIGA+YF+ G   A     N
Sbjct: 149 GVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSN-CNKKLIGAKYFINGF-LAENESFN 208

Query: 200 NSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQV 259
           ++   D+ SPRD  GHGTHVS++A G FVPN+SY GLA GTVRGGAP + IAMYK CW +
Sbjct: 209 STNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLAGGTVRGGAPRAHIAMYKACWYL 268

Query: 260 NGG---VCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGI 319
           +      C+  DILKA+D+A++DGVDVLS+SLG S P Y + D+R+GI  GAFHAV KGI
Sbjct: 269 DDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSSVPLYGETDIRDGITTGAFHAVLKGI 328

Query: 320 VVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQGMFSGKLTKF 379
            VV + GNSGP + +V+N  PW++TVAA+++DRSF   +TLGNN   +GQ M++G    F
Sbjct: 329 TVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFATPLTLGNNKVILGQAMYTGPGLGF 388

Query: 380 HNLVYPEVSDLDDER---ACESLSLN-DTWAAGNVVLCFASDDYNDDTHNTSSSVKKVGG 439
            +LVYPE     +E     CE L  N +    G VVLCF +  Y     + +  VK+ GG
Sbjct: 389 TSLVYPENPGNSNESFSGTCEELLFNSNRTMEGKVVLCFTTSPYGGAVLSAARYVKRAGG 448

Query: 440 LGLIVAKNPTKAVEPFVNNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVGQPLST 499
           LG+I+A++P  A++P +++FPCV +  ++G  IL Y RS+ +P VKI  S T VGQP+ T
Sbjct: 449 LGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILLYTRSSGSPVVKIQPSKTLVGQPVGT 508

Query: 500 TVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATPHVSAI 559
            VA FSSRGPNS+APAILKPDIAAPGV+ILAA   +   D+  +  +SGTSMA P +S +
Sbjct: 509 KVATFSSRGPNSIAPAILKPDIAAPGVSILAATTNTTFSDQ-GFIMLSGTSMAAPAISGV 568

Query: 560 VALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVNPNKAV 619
            ALLK LH  WSPAAI+SAIVTTAW +DP+G  IFAEG P   A PFD+GGG+VNP K+ 
Sbjct: 569 AALLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIFAEGSPPKLADPFDYGGGLVNPEKSA 628

Query: 620 DPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIPSLTHP 679
           +PGLVYDMG+ DY+ Y C+ GYN ++IS +  ++  C   +PS+LD N+PSITIP+L   
Sbjct: 629 NPGLVYDMGLEDYVLYMCSVGYNETSISQLIGKTTVCSNPKPSVLDFNLPSITIPNLKDE 688

Query: 680 VSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNHRVTTG 739
           V++TRTVTNVG +NS Y+  +E P G  + + P  L FN   K + F V +S+ H+  TG
Sbjct: 689 VTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPETLVFNSTTKKVYFKVKVSTTHKTNTG 748

Query: 740 YCFGSLTWLDGVHSVRIPVSVRTNI 758
           Y FGSLTW D +H+V IP+SVRT I
Sbjct: 749 YYFGSLTWSDSLHNVTIPLSVRTQI 770

BLAST of Csor.00g283160 vs. TAIR 10
Match: AT4G10550.3 (Subtilase family protein )

HSP 1 Score: 802.7 bits (2072), Expect = 2.5e-232
Identity = 401/745 (53.83%), Postives = 529/745 (71.01%), Query Frame = 0

Query: 20  AAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLT 79
           +AK  V+IVY+G+K HD+ E + ++HH +L S+LGS+E + DS+VYSY++GFSGFAAKLT
Sbjct: 45  SAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLT 104

Query: 80  MAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDT 139
            +QA+ I++LP VV VIP+  +K+ TTR+WDYL LS   P SLL ++ MG   IIGV+DT
Sbjct: 105 ESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAANPKSLLHETNMGEQIIIGVIDT 164

Query: 140 GIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLN 199
           G+WPESEVF D G GPVPS WKG CE+GE F+ +  C++KLIGA+YF+ G   A     N
Sbjct: 165 GVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSN-CNKKLIGAKYFINGF-LAENESFN 224

Query: 200 NSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQV 259
           ++   D+ SPRD  GHGTHVS++A G FVPN+SY GLA GTVRGGAP + IAMYK CW +
Sbjct: 225 STNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLAGGTVRGGAPRAHIAMYKACWYL 284

Query: 260 NGG---VCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGI 319
           +      C+  DILKA+D+A++DGVDVLS+SLG S P Y + D+R+GI  GAFHAV KGI
Sbjct: 285 DDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSSVPLYGETDIRDGITTGAFHAVLKGI 344

Query: 320 VVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQGMFSGKLTKF 379
            VV + GNSGP + +V+N  PW++TVAA+++DRSF   +TLGNN   +GQ M++G    F
Sbjct: 345 TVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFATPLTLGNNKVILGQAMYTGPGLGF 404

Query: 380 HNLVYPEVSDLDDER---ACESLSLN-DTWAAGNVVLCFASDDYNDDTHNTSSSVKKVGG 439
            +LVYPE     +E     CE L  N +    G VVLCF +  Y     + +  VK+ GG
Sbjct: 405 TSLVYPENPGNSNESFSGTCEELLFNSNRTMEGKVVLCFTTSPYGGAVLSAARYVKRAGG 464

Query: 440 LGLIVAKNPTKAVEPFVNNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVGQPLST 499
           LG+I+A++P  A++P +++FPCV +  ++G  IL Y RS+ +P VKI  S T VGQP+ T
Sbjct: 465 LGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILLYTRSSGSPVVKIQPSKTLVGQPVGT 524

Query: 500 TVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATPHVSAI 559
            VA FSSRGPNS+APAILKPDIAAPGV+ILAA   +   D+  +  +SGTSMA P +S +
Sbjct: 525 KVATFSSRGPNSIAPAILKPDIAAPGVSILAATTNTTFSDQ-GFIMLSGTSMAAPAISGV 584

Query: 560 VALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVNPNKAV 619
            ALLK LH  WSPAAI+SAIVTTAW +DP+G  IFAEG P   A PFD+GGG+VNP K+ 
Sbjct: 585 AALLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIFAEGSPPKLADPFDYGGGLVNPEKSA 644

Query: 620 DPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIPSLTHP 679
           +PGLVYDMG+ DY+ Y C+ GYN ++IS +  ++  C   +PS+LD N+PSITIP+L   
Sbjct: 645 NPGLVYDMGLEDYVLYMCSVGYNETSISQLIGKTTVCSNPKPSVLDFNLPSITIPNLKDE 704

Query: 680 VSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNHRVTTG 739
           V++TRTVTNVG +NS Y+  +E P G  + + P  L FN   K + F V +S+ H+  TG
Sbjct: 705 VTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPETLVFNSTTKKVYFKVKVSTTHKTNTG 764

Query: 740 YCFGSLTWLDGVHSVRIPVSVRTNI 758
           Y FGSLTW D +H+V IP+SVRT I
Sbjct: 765 YYFGSLTWSDSLHNVTIPLSVRTQI 786

BLAST of Csor.00g283160 vs. TAIR 10
Match: AT4G10540.1 (Subtilase family protein )

HSP 1 Score: 800.8 bits (2067), Expect = 9.3e-232
Identity = 407/756 (53.84%), Postives = 531/756 (70.24%), Query Frame = 0

Query: 9   LDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYK 68
           L+ L T      A+S V+IVY+G+K HD+ E + ++HH +L S+LGS+E +  S+V+SY+
Sbjct: 14  LNGLSTFVAHAGAESKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDAHSSMVHSYR 73

Query: 69  YGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSRM 128
           +GFSGFAAKLT +QA+ +++LP VV V P+  +++ TTR+WDYL LS   P +LL  + M
Sbjct: 74  HGFSGFAAKLTKSQAKKLADLPEVVHVTPDSFYQLDTTRTWDYLGLSVANPKNLLNDTNM 133

Query: 129 GSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVK 188
           G   IIG++D+G+WPESEVF D G+GPVPS WKG C SGE F+ ++ C++KLIGA+YF+ 
Sbjct: 134 GEEVIIGIVDSGVWPESEVFNDNGIGPVPSHWKGGCVSGENFTSSQ-CNKKLIGAKYFIN 193

Query: 189 GLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHS 248
           G  A +    N++E  D+ SPRDRSGHGTHV+++A G +VP++SY GLA GTVRGGAP +
Sbjct: 194 GFLATH-ESFNSTESLDFISPRDRSGHGTHVATIAGGSYVPSISYKGLAGGTVRGGAPRA 253

Query: 249 RIAMYKVCWQVNG---GVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIA 308
           RIAMYK CW ++      C+  DILKA+D+A++DGVDVLSLS+G  FP + + D+R  IA
Sbjct: 254 RIAMYKACWYLDRFDINTCSSADILKAMDEAMHDGVDVLSLSIGYRFPYFPETDVRAVIA 313

Query: 309 IGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMG 368
            GAFHAV KGI VV + GNSGPAA +V N  PW+LTVAA+++DRSF   ITLGNN   +G
Sbjct: 314 TGAFHAVLKGITVVCSGGNSGPAAQTVGNTAPWILTVAATTLDRSFPTPITLGNNKLILG 373

Query: 369 QGMFSGKLTKFHNLVYPEVSDLDDER---ACESLSLNDTWA-AGNVVLCFASDDYNDDTH 428
           Q M++G    F +LVYPE     +E     CE L  N     AG VVLCF +        
Sbjct: 374 QAMYTGPELGFTSLVYPENPGNSNESFSGDCELLFFNSNHTMAGKVVLCFTTSTRYITVS 433

Query: 429 NTSSSVKKVGGLGLIVAKNPTKAVEPFVNNFPCVQISLDIGMQILNYIRSTRNPQVKIGA 488
           +  S VK+ GGLG+IVA+NP   + P  ++FPCV +  ++G  IL YIRST  P VKI  
Sbjct: 434 SAVSYVKEAGGLGVIVARNPGDNLSPCEDDFPCVAVDYELGTDILLYIRSTGLPVVKIQP 493

Query: 489 STTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISG 548
           S T VGQP+ T VA FSSRGPNS+ PAILKPDIAAPGV+ILAA   +   +   + F+SG
Sbjct: 494 SKTLVGQPVGTKVADFSSRGPNSIEPAILKPDIAAPGVSILAATTTNKTFNDRGFIFLSG 553

Query: 549 TSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDF 608
           TSMA P +S +VALLK LH  WSPAAI+SAIVTTAW +DP+G  IFAEG P   A PFD+
Sbjct: 554 TSMAAPTISGVVALLKALHRDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKLADPFDY 613

Query: 609 GGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINV 668
           GGG+VNP KA  PGLVYD+G+ DY+ Y C+ GYN ++IS +  +   C   +PS+LD N+
Sbjct: 614 GGGLVNPEKAAKPGLVYDLGLEDYVLYMCSVGYNETSISQLVGKGTVCSNPKPSVLDFNL 673

Query: 669 PSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTV 728
           PSITIP+L   V+LTRT+TNVG + S YK  IE P GI + + P  L FN   K +SF V
Sbjct: 674 PSITIPNLKDEVTLTRTLTNVGQLESVYKVVIEPPIGIQVTVTPETLLFNSTTKRVSFKV 733

Query: 729 TISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 758
            +S+ H++ TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 734 KVSTTHKINTGYFFGSLTWSDSLHNVTIPLSVRTQI 767