Csor.00g280820 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g280820
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionPectinesterase
LocationCsor_Chr19: 10784389 .. 10785593 (+)
RNA-Seq ExpressionCsor.00g280820
SyntenyCsor.00g280820
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintronterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATTTCTATCCTTTGCTGCATGTTTATTTTTCTTCTTCACCAATGTTTTGGCACACGTTGATCACATCCGTTTTAATGTGGTTGTATCATTAGACGGATCGGGGGATTTTAAAAGTATTAGTGAAGCAATAGCAGCCGCTCCGAACAACTCCAATACACGATTTTACATTCGTGTATCCCCCGGCACTTACCGTGGACATCTTGAAATTCCACCAAACAAGAAATTTATTGCTTTGATTGGCGATAATGCACTCACAACCATTATCGTCGACAATAGAAGCCACGGTACTGGATTCTCGACTACTGACTCTGCAACCTTAGGTAATTACTCTGTTCTGTACTTTAATGTATTAATTTATTTGAGCAAAATAAATTTTACCTATTGACGTTAATATTTAACTAAAAAACTTGAACCCATCTCACCGTTCATTTTTGCTTTTAAATTAGGCTTTCTTTTTATATAGTCGTTTATATAAATAATATTTAACTAATTTCTTTATTTTTATTTGATGCAGTGGTGAATGGTGACCATTTTATGGCCCAACATTTAAGCTTTGAGAATTCTGCCGAACATCAAAACGGCCAAGTAGTTGCTGTCCTTAATCACGCGAAATTCACTACTTACTACAAGTGTAGATTCTTGAGTTTTCAAGATACCCTATATTTAAAGGGAGCATACCAATTCTTTAGGGAGTGTGACATCTACGGAACCGTAGATTTTATTTTTGGAAATGGACTTGCTATGTTTCAAGACTGTAACATATATGCTCGGTTACGGAGCAATGAAATCACAGTCACCGCACAGTCCAAGGAACAATCATGGGTGAAAAGCGGATTCTCCTTCCAGAATTGTACCGTCACAGTTTCTCCGAATATAGCTTCTAGAAAGGCTGATGTCAAAATCTTTCTTGGTCGACCATGGAGACAGTATTCAACTGTTGTGTTCATGGAGTCATTTTTGGATGACAACGTGCAACCAGAAGGATGGGTATTGTGGGAAGGGGTACCAATAAACAACCTCTTTTATGCTGAGTTCAATAATCGAGGTCCTGGTGCTGACACAACACATCGAGTTAATTGGACAAGTTTTCATGTACTCGATAAACAATTAGCGAAAAACTTTACCATAGAAAATTTTATCAACGGGTCAGATTGGTTGCCCGAAACACATGTACCCTTTAGAACTGGACTTGATGGTTAA

mRNA sequence

ATGGCATTTCTATCCTTTGCTGCATGTTTATTTTTCTTCTTCACCAATGTTTTGGCACACGTTGATCACATCCGTTTTAATGTGGTTGTATCATTAGACGGATCGGGGGATTTTAAAAGTATTAGTGAAGCAATAGCAGCCGCTCCGAACAACTCCAATACACGATTTTACATTCGTGTATCCCCCGGCACTTACCGTGGACATCTTGAAATTCCACCAAACAAGAAATTTATTGCTTTGATTGGCGATAATGCACTCACAACCATTATCGTCGACAATAGAAGCCACGGTACTGGATTCTCGACTACTGACTCTGCAACCTTAGTGGTGAATGGTGACCATTTTATGGCCCAACATTTAAGCTTTGAGAATTCTGCCGAACATCAAAACGGCCAAGTAGTTGCTGTCCTTAATCACGCGAAATTCACTACTTACTACAAGTGTAGATTCTTGAGTTTTCAAGATACCCTATATTTAAAGGGAGCATACCAATTCTTTAGGGAGTGTGACATCTACGGAACCGTAGATTTTATTTTTGGAAATGGACTTGCTATGTTTCAAGACTGTAACATATATGCTCGGTTACGGAGCAATGAAATCACAGTCACCGCACAGTCCAAGGAACAATCATGGGTGAAAAGCGGATTCTCCTTCCAGAATTGTACCGTCACAGTTTCTCCGAATATAGCTTCTAGAAAGGCTGATGTCAAAATCTTTCTTGGTCGACCATGGAGACAGTATTCAACTGTTGTGTTCATGGAGTCATTTTTGGATGACAACGTGCAACCAGAAGGATGGGTATTGTGGGAAGGGGTACCAATAAACAACCTCTTTTATGCTGAGTTCAATAATCGAGGTCCTGGTGCTGACACAACACATCGAGTTAATTGGACAAGTTTTCATGTACTCGATAAACAATTAGCGAAAAACTTTACCATAGAAAATTTTATCAACGGGTCAGATTGGTTGCCCGAAACACATGTACCCTTTAGAACTGGACTTGATGGTTAA

Coding sequence (CDS)

ATGGCATTTCTATCCTTTGCTGCATGTTTATTTTTCTTCTTCACCAATGTTTTGGCACACGTTGATCACATCCGTTTTAATGTGGTTGTATCATTAGACGGATCGGGGGATTTTAAAAGTATTAGTGAAGCAATAGCAGCCGCTCCGAACAACTCCAATACACGATTTTACATTCGTGTATCCCCCGGCACTTACCGTGGACATCTTGAAATTCCACCAAACAAGAAATTTATTGCTTTGATTGGCGATAATGCACTCACAACCATTATCGTCGACAATAGAAGCCACGGTACTGGATTCTCGACTACTGACTCTGCAACCTTAGTGGTGAATGGTGACCATTTTATGGCCCAACATTTAAGCTTTGAGAATTCTGCCGAACATCAAAACGGCCAAGTAGTTGCTGTCCTTAATCACGCGAAATTCACTACTTACTACAAGTGTAGATTCTTGAGTTTTCAAGATACCCTATATTTAAAGGGAGCATACCAATTCTTTAGGGAGTGTGACATCTACGGAACCGTAGATTTTATTTTTGGAAATGGACTTGCTATGTTTCAAGACTGTAACATATATGCTCGGTTACGGAGCAATGAAATCACAGTCACCGCACAGTCCAAGGAACAATCATGGGTGAAAAGCGGATTCTCCTTCCAGAATTGTACCGTCACAGTTTCTCCGAATATAGCTTCTAGAAAGGCTGATGTCAAAATCTTTCTTGGTCGACCATGGAGACAGTATTCAACTGTTGTGTTCATGGAGTCATTTTTGGATGACAACGTGCAACCAGAAGGATGGGTATTGTGGGAAGGGGTACCAATAAACAACCTCTTTTATGCTGAGTTCAATAATCGAGGTCCTGGTGCTGACACAACACATCGAGTTAATTGGACAAGTTTTCATGTACTCGATAAACAATTAGCGAAAAACTTTACCATAGAAAATTTTATCAACGGGTCAGATTGGTTGCCCGAAACACATGTACCCTTTAGAACTGGACTTGATGGTTAA

Protein sequence

MAFLSFAACLFFFFTNVLAHVDHIRFNVVVSLDGSGDFKSISEAIAAAPNNSNTRFYIRVSPGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVVNGDHFMAQHLSFENSAEHQNGQVVAVLNHAKFTTYYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFGNGLAMFQDCNIYARLRSNEITVTAQSKEQSWVKSGFSFQNCTVTVSPNIASRKADVKIFLGRPWRQYSTVVFMESFLDDNVQPEGWVLWEGVPINNLFYAEFNNRGPGADTTHRVNWTSFHVLDKQLAKNFTIENFINGSDWLPETHVPFRTGLDG
Homology
BLAST of Csor.00g280820 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 1.6e-74
Identity = 138/313 (44.09%), Postives = 197/313 (62.94%), Query Frame = 0

Query: 25  RFNVVVSLDGSGDFKSISEAIAAAPNNSNTRFYIRVSPGTYRGHLEIPPNKKFIALIGDN 84
           R N+VV+ DGSG+ K+I EA+AAA     +R+ I +  GTY  ++E+    K I  +GD 
Sbjct: 200 RANIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENIEV--KLKNIMFVGDG 259

Query: 85  ALTTIIVDNRSHGTGFSTTDSATLVVNGDHFMAQHLSFENSAEHQNGQVVAVLNHAKFTT 144
              TII  ++S G G +T  SAT+ V GD+F+A+ ++  N+A   N Q VA+ + +  + 
Sbjct: 260 IGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSV 319

Query: 145 YYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFGNGLAMFQDCNIYARLRSNEI-TVT 204
           +Y+C F  +QDTLY+    QF+RECDIYGTVDFIFGN   + Q+CNI+AR   N   T+T
Sbjct: 320 FYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPNRTNTLT 379

Query: 205 AQSKEQSWVKSGFSFQNCTVTVSPNIASRKADVKIFLGRPWRQYSTVVFMESFLDDNVQP 264
           AQ +      +G    NC VT + ++   ++ VK FLGRPW+QYS  V++++FLD  + P
Sbjct: 380 AQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINP 439

Query: 265 EGWVLWEG-VPINNLFYAEFNNRGPGADTTHRVNWTSFHVL-DKQLAKNFTIENFINGSD 324
            GW+ W G   +N L+YAE+ N GPG+ T +RV W  +HVL        FT+ NFI G+ 
Sbjct: 440 AGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNS 499

Query: 325 WLPETHVPFRTGL 335
           WLP T+VPF +GL
Sbjct: 500 WLPATNVPFTSGL 510

BLAST of Csor.00g280820 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 4.5e-74
Identity = 132/313 (42.17%), Postives = 202/313 (64.54%), Query Frame = 0

Query: 28  VVVSLDGSGDFKSISEAIAAAPNNSNTR---FYIRVSPGTYRGHLEIPPNKKFIALIGDN 87
           V VS DG+G+F +I+ A+AAAPNN++     F I V+ G Y  ++ I  NK+++ +IGD 
Sbjct: 265 VTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDG 324

Query: 88  ALTTIIVDNRSHGTGFSTTDSATLVVNGDHFMAQHLSFENSAEHQNGQVVAVLNHAKFTT 147
              T++  NRS   G++T +SAT  V   +F+A +++F N+A  +  Q VA+ + A F+ 
Sbjct: 325 INQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSI 384

Query: 148 YYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFGNGLAMFQDCNIYAR--LRSNEITV 207
           +Y C F ++QDTLY     QF+RECD+YGTVDFIFGN   +FQ+CN+Y R  + +    +
Sbjct: 385 FYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAI 444

Query: 208 TAQSKEQSWVKSGFSFQNCTVTVSPNIASRKADVKIFLGRPWRQYSTVVFMESFLDDNVQ 267
           TAQ +      +G S QNCT+  + ++ S    VK +LGRPW++YS  V+M+S++D  V+
Sbjct: 445 TAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVE 504

Query: 268 PEGWVLWEG-VPINNLFYAEFNNRGPGADTTHRVNWTSFHVLDKQLAKNFTIENFINGSD 327
           P GW  W G   ++ L+YAE+NN GPG++TT+RV W  +HV++   A NFT+      +D
Sbjct: 505 PVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANFTVTGLFIEAD 564

Query: 328 WLPETHVPFRTGL 335
           W+ +T VP+ +GL
Sbjct: 565 WIWKTGVPYTSGL 577

BLAST of Csor.00g280820 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 278.5 bits (711), Expect = 1.0e-73
Identity = 133/315 (42.22%), Postives = 201/315 (63.81%), Query Frame = 0

Query: 28  VVVSLDGSGDFKSISEAIAAAPN---NSNTRFYIRVSPGTYRGHLEIPPNKKFIALIGDN 87
           V V  +G+G+F +I+ AIAAAPN    SN  F I V+ G Y  ++E+P NK+++ +IGD 
Sbjct: 246 VTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDG 305

Query: 88  ALTTIIVDNRSHGTGFSTTDSATLVVNGDHFMAQHLSFENSAEHQNGQVVAVLNHAKFTT 147
              T+I  NRS   G++T +SAT +++G +F+  +++  N+A    GQ VA+ +    + 
Sbjct: 306 INQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSV 365

Query: 148 YYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFGNGLAMFQDCNIYARL----RSNEI 207
           +Y C F ++QDTLY     QF+RECD+YGTVDFIFGN   + Q+CN+Y R     +SNE 
Sbjct: 366 FYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNE- 425

Query: 208 TVTAQSKEQSWVKSGFSFQNCTVTVSPNIASRKADVKIFLGRPWRQYSTVVFMESFLDDN 267
            VTAQ +      +G +   CT+  + ++A+    VK +LGRPW++YS  V M++++D  
Sbjct: 426 -VTAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGF 485

Query: 268 VQPEGWVLWEG-VPINNLFYAEFNNRGPGADTTHRVNWTSFHVLDKQLAKNFTIENFING 327
           ++P GW  W G   ++ L+YAE+NN GPG+DTT+RV W  +HV++   A NFT+ NF+ G
Sbjct: 486 LEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVG 545

Query: 328 SDWLPETHVPFRTGL 335
             W+ +T VPF  GL
Sbjct: 546 EGWIGQTGVPFVGGL 558

BLAST of Csor.00g280820 vs. ExPASy Swiss-Prot
Match: O48711 (Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PME12 PE=2 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 6.5e-73
Identity = 136/311 (43.73%), Postives = 194/311 (62.38%), Query Frame = 0

Query: 27  NVVVSLDGSGDFKSISEAIAAAPNNSNTRFYIRVSPGTYRGHLEIPPNKKFIALIGDNAL 86
           ++VV+ DG+G+F +I+EAI+ APN SN R  I V  G Y  +++IP  K  I LIGD + 
Sbjct: 237 SLVVAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDGSD 296

Query: 87  TTIIVDNRSHGTGFSTTDSATLVVNGDHFMAQHLSFENSAEHQNGQVVAVLNHAKFTTYY 146
            T I  NRS G G++T  SATL V+G+ F+A+ +   N+A  +  Q VA+  +A F   Y
Sbjct: 297 VTFITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVALY 356

Query: 147 KCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFGNGLAMFQDCNIYARL-RSNEITV-TA 206
           +C    +QDTLY     QF+RECDIYGT+D+IFGN   +FQ CNI ++L    + TV TA
Sbjct: 357 RCVIDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVITA 416

Query: 207 QSKEQSWVKSGFSFQNCTVTVSPNIASRKADVKIFLGRPWRQYSTVVFMESFLDDNVQPE 266
           QS++     +G S QNC++  S ++ +    VK +LGRPWR++S  V MES++D+ +   
Sbjct: 417 QSRDTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDEFIDGS 476

Query: 267 GWVLWE-GVPINNLFYAEFNNRGPGADTTHRVNWTSFHVLDKQLAKNFTIENFINGSDWL 326
           GW  W  G  ++ L+Y E+NN GPG++T  RVNW  FH++  + A NFT   FI G  WL
Sbjct: 477 GWSKWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIMGYEDAFNFTATEFITGDGWL 536

Query: 327 PETHVPFRTGL 335
             T  P+  G+
Sbjct: 537 GSTSFPYDNGI 547

BLAST of Csor.00g280820 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 8.5e-73
Identity = 133/313 (42.49%), Postives = 197/313 (62.94%), Query Frame = 0

Query: 28  VVVSLDGSGDFKSISEAIAAAPNNSNTR---FYIRVSPGTYRGHLEIPPNKKFIALIGDN 87
           V+V    S +F +I++AIAAAPNN+      F I    G Y  ++ +P NKK + L+GD 
Sbjct: 303 VIVGPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDG 362

Query: 88  ALTTIIVDNRSHGTGFSTTDSATLVVNGDHFMAQHLSFENSAEHQNGQVVAVLNHAKFTT 147
              TII  N +   G++T + ++  V G+ FMA  ++F N+A  +  Q VA+ N+A+ ++
Sbjct: 363 INKTIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSS 422

Query: 148 YYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFGNGLAMFQDCNIYAR--LRSNEITV 207
           +Y+C F  +QDTLY+    QF+RECDIYGTVDFIFGN  A+FQ+CNIYAR  +   +  +
Sbjct: 423 FYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAI 482

Query: 208 TAQSKEQSWVKSGFSFQNCTVTVSPNIASRKADVKIFLGRPWRQYSTVVFMESFLDDNVQ 267
           TA  +      +G S  NCT+  +P++A+       FLGRPW+ YS  VFM+S++ D VQ
Sbjct: 483 TAHGRLDPNQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQ 542

Query: 268 PEGWVLWEG-VPINNLFYAEFNNRGPGADTTHRVNWTSFHVLDKQLAKNFTIENFINGSD 327
           P GW+ W G + ++ ++Y E++N GPGA+T  RV W  +++L+   A NFT+ NF  G  
Sbjct: 543 PVGWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDT 602

Query: 328 WLPETHVPFRTGL 335
           WLP+T +PF  GL
Sbjct: 603 WLPQTDIPFYGGL 615

BLAST of Csor.00g280820 vs. NCBI nr
Match: KAG6572379.1 (Pectinesterase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 693 bits (1788), Expect = 6.49e-252
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0

Query: 1   MAFLSFAACLFFFFTNVLAHVDHIRFNVVVSLDGSGDFKSISEAIAAAPNNSNTRFYIRV 60
           MAFLSFAACLFFFFTNVLAHVDHIRFNVVVSLDGSGDFKSISEAIAAAPNNSNTRFYIRV
Sbjct: 1   MAFLSFAACLFFFFTNVLAHVDHIRFNVVVSLDGSGDFKSISEAIAAAPNNSNTRFYIRV 60

Query: 61  SPGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVVNGDHFMAQHL 120
           SPGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVVNGDHFMAQHL
Sbjct: 61  SPGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVVNGDHFMAQHL 120

Query: 121 SFENSAEHQNGQVVAVLNHAKFTTYYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFG 180
           SFENSAEHQNGQVVAVLNHAKFTTYYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFG
Sbjct: 121 SFENSAEHQNGQVVAVLNHAKFTTYYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFG 180

Query: 181 NGLAMFQDCNIYARLRSNEITVTAQSKEQSWVKSGFSFQNCTVTVSPNIASRKADVKIFL 240
           NGLAMFQDCNIYARLRSNEITVTAQSKEQSWVKSGFSFQNCTVTVSPNIASRKADVKIFL
Sbjct: 181 NGLAMFQDCNIYARLRSNEITVTAQSKEQSWVKSGFSFQNCTVTVSPNIASRKADVKIFL 240

Query: 241 GRPWRQYSTVVFMESFLDDNVQPEGWVLWEGVPINNLFYAEFNNRGPGADTTHRVNWTSF 300
           GRPWRQYSTVVFMESFLDDNVQPEGWVLWEGVPINNLFYAEFNNRGPGADTTHRVNWTSF
Sbjct: 241 GRPWRQYSTVVFMESFLDDNVQPEGWVLWEGVPINNLFYAEFNNRGPGADTTHRVNWTSF 300

Query: 301 HVLDKQLAKNFTIENFINGSDWLPETHVPFRTGLDG 336
           HVLDKQLAKNFTIENFINGSDWLPETHVPFRTGLDG
Sbjct: 301 HVLDKQLAKNFTIENFINGSDWLPETHVPFRTGLDG 336

BLAST of Csor.00g280820 vs. NCBI nr
Match: XP_022952744.1 (pectinesterase 2-like [Cucurbita moschata])

HSP 1 Score: 678 bits (1749), Expect = 5.74e-246
Identity = 325/336 (96.73%), Postives = 332/336 (98.81%), Query Frame = 0

Query: 1   MAFLSFAACLFFFFTNVLAHVDHIRFNVVVSLDGSGDFKSISEAIAAAPNNSNTRFYIRV 60
           MAFLSFAACLFFFF N+LAHVDHIRFN VVSLDGSGDFKSISEAIAAAP+NSNTRFYIRV
Sbjct: 1   MAFLSFAACLFFFFANILAHVDHIRFNAVVSLDGSGDFKSISEAIAAAPDNSNTRFYIRV 60

Query: 61  SPGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVVNGDHFMAQHL 120
           SPGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVVNGDH+MAQHL
Sbjct: 61  SPGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVVNGDHYMAQHL 120

Query: 121 SFENSAEHQNGQVVAVLNHAKFTTYYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFG 180
           SFENSAEHQNGQ VAVLNH+KFTTYYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFG
Sbjct: 121 SFENSAEHQNGQAVAVLNHSKFTTYYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFG 180

Query: 181 NGLAMFQDCNIYARLRSNEITVTAQSKEQSWVKSGFSFQNCTVTVSPNIASRKADVKIFL 240
           NGLAMFQDCNIYARL+SNEITVTAQSKEQSWVKSGFSFQNCTVTVSP+IASRK DVKIFL
Sbjct: 181 NGLAMFQDCNIYARLQSNEITVTAQSKEQSWVKSGFSFQNCTVTVSPDIASRKVDVKIFL 240

Query: 241 GRPWRQYSTVVFMESFLDDNVQPEGWVLWEGVPINNLFYAEFNNRGPGADTTHRVNWTSF 300
           GRPWRQYSTVVFMESFLDDNVQPEGWVLWEGVPINNLFYAEFNNRGPGADTTHRVNWTSF
Sbjct: 241 GRPWRQYSTVVFMESFLDDNVQPEGWVLWEGVPINNLFYAEFNNRGPGADTTHRVNWTSF 300

Query: 301 HVLDKQLAKNFTIENFINGSDWLPETHVPFRTGLDG 336
           HVLDKQLAKNFT+ENFINGSDWLPETHVPFRTGLDG
Sbjct: 301 HVLDKQLAKNFTVENFINGSDWLPETHVPFRTGLDG 336

BLAST of Csor.00g280820 vs. NCBI nr
Match: XP_023511444.1 (probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 609 bits (1570), Expect = 1.10e-218
Identity = 294/336 (87.50%), Postives = 313/336 (93.15%), Query Frame = 0

Query: 1   MAFLSFAACLFFFFTNVLAHVDHIRFNVVVSLDGSGDFKSISEAIAAAPNNSNTRFYIRV 60
           MAFL FAACLFFFF NVLA +D  RFN VVSLDGSGDFKSISEAIAAAPNNSNTRFYIRV
Sbjct: 1   MAFLPFAACLFFFFANVLALIDPTRFNAVVSLDGSGDFKSISEAIAAAPNNSNTRFYIRV 60

Query: 61  SPGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVVNGDHFMAQHL 120
           +PGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGF T DSATLVV GD+FMAQ+L
Sbjct: 61  APGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFKTFDSATLVVKGDNFMAQNL 120

Query: 121 SFENSAEHQNGQVVAVLNHAKFTTYYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFG 180
           SFENSAE QNGQ VA+L+ AKFTTY++CRFLSFQDTLY KG  QFF+ CDIYGTVDFIFG
Sbjct: 121 SFENSAEPQNGQAVAILDRAKFTTYFQCRFLSFQDTLYTKGINQFFKNCDIYGTVDFIFG 180

Query: 181 NGLAMFQDCNIYARLRSNEITVTAQSKEQSWVKSGFSFQNCTVTVSPNIASRKADVKIFL 240
           +GLA+FQDCNIYARL  NEITVTAQSK+QSW+KSGFSFQNCTVTVSP IASRKADVKIFL
Sbjct: 181 DGLAVFQDCNIYARLPLNEITVTAQSKDQSWLKSGFSFQNCTVTVSPEIASRKADVKIFL 240

Query: 241 GRPWRQYSTVVFMESFLDDNVQPEGWVLWEGVPINNLFYAEFNNRGPGADTTHRVNWTSF 300
           GRPWRQYSTVVFMESFLDDNVQP+GW LWEGVPINNLFYAEFNNRGPGADTTHRVNW +F
Sbjct: 241 GRPWRQYSTVVFMESFLDDNVQPQGWELWEGVPINNLFYAEFNNRGPGADTTHRVNWPTF 300

Query: 301 HVLDKQLAKNFTIENFINGSDWLPETHVPFRTGLDG 336
           H+L+KQLA+NFT+ENFIN SDWL ETHVPFRTGLDG
Sbjct: 301 HILNKQLARNFTVENFINESDWLLETHVPFRTGLDG 336

BLAST of Csor.00g280820 vs. NCBI nr
Match: XP_023511662.1 (probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 602 bits (1551), Expect = 8.65e-216
Identity = 291/335 (86.87%), Postives = 312/335 (93.13%), Query Frame = 0

Query: 1   MAFLSFAACLFFFFTNVLAHVDHIRFNVVVSLDGSGDFKSISEAIAAAPNNSNTRFYIRV 60
           MAFL FAACLFFFF NVLA +D  RFN VVSLDGSGDFKSISEAIAAAP+NSNTRFYIRV
Sbjct: 1   MAFLPFAACLFFFFANVLALIDPARFNAVVSLDGSGDFKSISEAIAAAPDNSNTRFYIRV 60

Query: 61  SPGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVVNGDHFMAQHL 120
           +PGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVV G +FMAQHL
Sbjct: 61  TPGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVVRGHNFMAQHL 120

Query: 121 SFENSAEHQNGQVVAVLNHAKFTTYYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFG 180
           SFENSA  QNGQ VAVL++AK+T YY CRFLSFQDTLY +G  QFFREC++YGTVDFIFG
Sbjct: 121 SFENSAGPQNGQAVAVLDYAKYTVYYDCRFLSFQDTLYSRGQCQFFRECEVYGTVDFIFG 180

Query: 181 NGLAMFQDCNIYARLRSNEITVTAQSKEQSWVKSGFSFQNCTVTVSPNIASRKADVKIFL 240
           +GLA+FQDCNIYARL S+EITVTAQSK++SW KSGFSFQNCTVTVSP IAS K  VKI+L
Sbjct: 181 DGLAVFQDCNIYARLPSDEITVTAQSKDRSWWKSGFSFQNCTVTVSPEIASSKDKVKIYL 240

Query: 241 GRPWRQYSTVVFMESFLDDNVQPEGWVLWEGVPINNLFYAEFNNRGPGADTTHRVNWTSF 300
           GRPWRQYSTVVFMESFLDDNV+PEGWVLWEGVPINNLFYAEFNNRGPGADTTHRVNW++F
Sbjct: 241 GRPWRQYSTVVFMESFLDDNVRPEGWVLWEGVPINNLFYAEFNNRGPGADTTHRVNWSTF 300

Query: 301 HVLDKQLAKNFTIENFINGSDWLPETHVPFRTGLD 335
           HVLDKQLAKNFT++ FINGSDWLPETHVPFRTGLD
Sbjct: 301 HVLDKQLAKNFTVKFFINGSDWLPETHVPFRTGLD 335

BLAST of Csor.00g280820 vs. NCBI nr
Match: KAG6572378.1 (putative pectinesterase/pectinesterase inhibitor 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 598 bits (1543), Expect = 1.43e-214
Identity = 291/336 (86.61%), Postives = 312/336 (92.86%), Query Frame = 0

Query: 1   MAFLSFAACLFFFFTNVLAHVDHIRFNVVVSLDGSGDFKSISEAIAAAPNNSNTRFYIRV 60
           MAFLSFAACLFFFF NVLA +D  RFN VVSLDGSGDFKSISEAIAAAPNNSNTRFYIRV
Sbjct: 1   MAFLSFAACLFFFFANVLALIDPTRFNAVVSLDGSGDFKSISEAIAAAPNNSNTRFYIRV 60

Query: 61  SPGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVVNGDHFMAQHL 120
           +PGTYRGHLEI PNKKFIALIGDNALTTIIVDNRSHGTGF T++SATLVVNGD+FMAQHL
Sbjct: 61  TPGTYRGHLEILPNKKFIALIGDNALTTIIVDNRSHGTGFKTSESATLVVNGDNFMAQHL 120

Query: 121 SFENSAEHQNGQVVAVLNHAKFTTYYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFG 180
           SFENSA  QNGQ VAVL++AK T YY CRFLSFQDTLY +G  QFFRECD+YGTVDFIFG
Sbjct: 121 SFENSAGPQNGQAVAVLDYAKHTVYYDCRFLSFQDTLYSRGECQFFRECDVYGTVDFIFG 180

Query: 181 NGLAMFQDCNIYARLRSNEITVTAQSKEQSWVKSGFSFQNCTVTVSPNIASRKADVKIFL 240
           +GLA+FQDCNIYARLRS+EITVTAQSKE+S  +S FSFQNCTVTVSPNI  RKA+VKI+L
Sbjct: 181 DGLAVFQDCNIYARLRSDEITVTAQSKEKSESQSVFSFQNCTVTVSPNITYRKAEVKIYL 240

Query: 241 GRPWRQYSTVVFMESFLDDNVQPEGWVLWEGVPINNLFYAEFNNRGPGADTTHRVNWTSF 300
           GRPWRQYSTVVFMESFLDDNVQP GW+LWEGVPIN+LFYAEFNNRGPGA+TTHRVNW +F
Sbjct: 241 GRPWRQYSTVVFMESFLDDNVQPAGWILWEGVPINHLFYAEFNNRGPGANTTHRVNWPTF 300

Query: 301 HVLDKQLAKNFTIENFINGSDWLPETHVPFRTGLDG 336
           HVLDKQLAKNFT+ENFINGSDWLPET VPFRTGLDG
Sbjct: 301 HVLDKQLAKNFTVENFINGSDWLPETRVPFRTGLDG 336

BLAST of Csor.00g280820 vs. ExPASy TrEMBL
Match: A0A6J1GMM0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455349 PE=4 SV=1)

HSP 1 Score: 678 bits (1749), Expect = 2.78e-246
Identity = 325/336 (96.73%), Postives = 332/336 (98.81%), Query Frame = 0

Query: 1   MAFLSFAACLFFFFTNVLAHVDHIRFNVVVSLDGSGDFKSISEAIAAAPNNSNTRFYIRV 60
           MAFLSFAACLFFFF N+LAHVDHIRFN VVSLDGSGDFKSISEAIAAAP+NSNTRFYIRV
Sbjct: 1   MAFLSFAACLFFFFANILAHVDHIRFNAVVSLDGSGDFKSISEAIAAAPDNSNTRFYIRV 60

Query: 61  SPGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVVNGDHFMAQHL 120
           SPGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVVNGDH+MAQHL
Sbjct: 61  SPGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVVNGDHYMAQHL 120

Query: 121 SFENSAEHQNGQVVAVLNHAKFTTYYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFG 180
           SFENSAEHQNGQ VAVLNH+KFTTYYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFG
Sbjct: 121 SFENSAEHQNGQAVAVLNHSKFTTYYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFG 180

Query: 181 NGLAMFQDCNIYARLRSNEITVTAQSKEQSWVKSGFSFQNCTVTVSPNIASRKADVKIFL 240
           NGLAMFQDCNIYARL+SNEITVTAQSKEQSWVKSGFSFQNCTVTVSP+IASRK DVKIFL
Sbjct: 181 NGLAMFQDCNIYARLQSNEITVTAQSKEQSWVKSGFSFQNCTVTVSPDIASRKVDVKIFL 240

Query: 241 GRPWRQYSTVVFMESFLDDNVQPEGWVLWEGVPINNLFYAEFNNRGPGADTTHRVNWTSF 300
           GRPWRQYSTVVFMESFLDDNVQPEGWVLWEGVPINNLFYAEFNNRGPGADTTHRVNWTSF
Sbjct: 241 GRPWRQYSTVVFMESFLDDNVQPEGWVLWEGVPINNLFYAEFNNRGPGADTTHRVNWTSF 300

Query: 301 HVLDKQLAKNFTIENFINGSDWLPETHVPFRTGLDG 336
           HVLDKQLAKNFT+ENFINGSDWLPETHVPFRTGLDG
Sbjct: 301 HVLDKQLAKNFTVENFINGSDWLPETHVPFRTGLDG 336

BLAST of Csor.00g280820 vs. ExPASy TrEMBL
Match: A0A6J1GM54 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455508 PE=4 SV=1)

HSP 1 Score: 594 bits (1532), Expect = 3.29e-213
Identity = 287/336 (85.42%), Postives = 308/336 (91.67%), Query Frame = 0

Query: 1   MAFLSFAACLFFFFTNVLAHVDHIRFNVVVSLDGSGDFKSISEAIAAAPNNSNTRFYIRV 60
           MAFL  AACLFFFF NVLA VD IRFN VVSLDGSGDFKSISEAIAAAPNNSNTRFYIRV
Sbjct: 1   MAFLQLAACLFFFFANVLALVDSIRFNAVVSLDGSGDFKSISEAIAAAPNNSNTRFYIRV 60

Query: 61  SPGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVVNGDHFMAQHL 120
           +PG YRGHLEIPP KKFIALIGD+ALTTIIVDNRSHGTGF T++SATLVVNGD+FMAQHL
Sbjct: 61  TPGIYRGHLEIPPAKKFIALIGDSALTTIIVDNRSHGTGFKTSESATLVVNGDNFMAQHL 120

Query: 121 SFENSAEHQNGQVVAVLNHAKFTTYYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFG 180
           SFENSAE QNGQ VAVL++AK + YY CRF+SFQDTLY KG +QFFRECDIYGTVDFIFG
Sbjct: 121 SFENSAEPQNGQAVAVLDYAKHSVYYDCRFMSFQDTLYSKGEFQFFRECDIYGTVDFIFG 180

Query: 181 NGLAMFQDCNIYARLRSNEITVTAQSKEQSWVKSGFSFQNCTVTVSPNIASRKADVKIFL 240
           NGLA+FQDCNIYARL  +EITVTAQSKEQS  +SGFSFQNCTVTVSP I + K  VKI+L
Sbjct: 181 NGLAVFQDCNIYARLPMDEITVTAQSKEQSVSQSGFSFQNCTVTVSPEITANKTKVKIYL 240

Query: 241 GRPWRQYSTVVFMESFLDDNVQPEGWVLWEGVPINNLFYAEFNNRGPGADTTHRVNWTSF 300
           GRPWR+YSTVVFMESFLDDNVQP+GW+ WEGVPINNLFYAEFNNRGPGA+TTHRVNW +F
Sbjct: 241 GRPWREYSTVVFMESFLDDNVQPQGWISWEGVPINNLFYAEFNNRGPGANTTHRVNWPTF 300

Query: 301 HVLDKQLAKNFTIENFINGSDWLPETHVPFRTGLDG 336
           HVLDK LAKNFT+ENFINGSDWLPETHVPFRTGLDG
Sbjct: 301 HVLDKLLAKNFTVENFINGSDWLPETHVPFRTGLDG 336

BLAST of Csor.00g280820 vs. ExPASy TrEMBL
Match: A0A6J1GK92 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455081 PE=4 SV=1)

HSP 1 Score: 523 bits (1348), Expect = 3.36e-185
Identity = 251/334 (75.15%), Postives = 288/334 (86.23%), Query Frame = 0

Query: 1   MAFLSFAACLFFFFTNVLAHVDHIRFNVVVSLDGSGDFKSISEAIAAAPNNSNTRFYIRV 60
           MAF    +C F  F N LA VD   FN  V+LDG+G+FKSI++AIAAAPNNSNTRFYIRV
Sbjct: 1   MAFFQRISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV 60

Query: 61  SPGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVVNGDHFMAQHL 120
           SPG Y  HLEI   KKFIALIG+N  TTIIVDNRS+  GF T++SAT+VV G++FMAQHL
Sbjct: 61  SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL 120

Query: 121 SFENSAEHQNGQVVAVLNHAKFTTYYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFG 180
           SFENSA+HQ+GQ VAVL+ AK+T YYKCRFLSFQDTLY KG YQFF+ CDIYGTVDFIFG
Sbjct: 121 SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG 180

Query: 181 NGLAMFQDCNIYARLRSNEITVTAQSKEQSWVKSGFSFQNCTVTVSPNIASRKADVKIFL 240
           NGLAMFQDCN+YARL SN+ TVTAQSK+Q W +SGFSFQNCTVTVSP IAS KA VKI+L
Sbjct: 181 NGLAMFQDCNVYARLPSNDSTVTAQSKKQ-WTQSGFSFQNCTVTVSPEIASCKALVKIYL 240

Query: 241 GRPWRQYSTVVFMESFLDDNVQPEGWVLWEGVPINNLFYAEFNNRGPGADTTHRVNWTSF 300
           GRPW+ YSTVVFMESFLDDNVQP+GW+LW GVP+NNLFYAE+NNRGPGA+TTHRVNW  F
Sbjct: 241 GRPWKPYSTVVFMESFLDDNVQPQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPGF 300

Query: 301 HVLDKQLAKNFTIENFINGSDWLPETHVPFRTGL 334
           HV+D+QLAK+FT+ENFING+ WLPET+VP+ +GL
Sbjct: 301 HVIDRQLAKSFTVENFINGTYWLPETNVPYTSGL 333

BLAST of Csor.00g280820 vs. ExPASy TrEMBL
Match: A0A6J1HZD2 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111468060 PE=4 SV=1)

HSP 1 Score: 521 bits (1341), Expect = 1.63e-184
Identity = 263/335 (78.51%), Postives = 279/335 (83.28%), Query Frame = 0

Query: 1   MAFLSFAACLFFFFTNVLAHVDHIRFNVVVSLDGSGDFKSISEAIAAAPNNSNTRFYIRV 60
           M FL  AACLFFFF NVLA VD IRFN VVSLDGSGDFKSISEAIAAAP+NSN RFYI V
Sbjct: 1   MTFLQLAACLFFFFVNVLALVDPIRFNAVVSLDGSGDFKSISEAIAAAPDNSNARFYIHV 60

Query: 61  SPGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVVNGDHFMAQHL 120
           +PG YRGHLEIPP KKFIALIGDNALTTIIVDNRSHGTGF T++SAT+VVNGD+FMAQHL
Sbjct: 61  TPGIYRGHLEIPPAKKFIALIGDNALTTIIVDNRSHGTGFKTSESATVVVNGDNFMAQHL 120

Query: 121 SFENSAEHQNGQVVAVLNHAKFTTYYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFG 180
           SFENSAE QNGQ VAVL++AK T YY CRFLSFQDTLY KG  QFFRECDIYGTVDFIFG
Sbjct: 121 SFENSAEPQNGQAVAVLDYAKHTVYYDCRFLSFQDTLYSKGERQFFRECDIYGTVDFIFG 180

Query: 181 NGLAMFQDCNIYARLRSNEITVTAQSKEQSWVKSGFSFQNCTVTVSPNIASRKADVKIFL 240
           NGLA+FQDCNIYARL  +EITVTAQSKEQS  +SGFSFQNCTVTVSP IA+ K  VKI+L
Sbjct: 181 NGLAVFQDCNIYARLPMDEITVTAQSKEQSISQSGFSFQNCTVTVSPEIAANKTKVKIYL 240

Query: 241 GRPWRQYSTVVFMESFLDDNVQPEGWVLWEGVPINNLFYAEFNNRGPGADTTHRVNWTSF 300
           GRPWR YSTVVFMESFLDDNVQ +GW+LWEGVPINNLFYAEFNNRGPGADTTHRVNW   
Sbjct: 241 GRPWRPYSTVVFMESFLDDNVQLQGWILWEGVPINNLFYAEFNNRGPGADTTHRVNW--- 300

Query: 301 HVLDKQLAKNFTIENFINGSDWLPETHVPFRTGLD 335
                                 LPET VPFRTGL+
Sbjct: 301 ----------------------LPETRVPFRTGLN 310

BLAST of Csor.00g280820 vs. ExPASy TrEMBL
Match: A0A6J1GLJ0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455081 PE=4 SV=1)

HSP 1 Score: 513 bits (1321), Expect = 3.24e-181
Identity = 247/327 (75.54%), Postives = 282/327 (86.24%), Query Frame = 0

Query: 1   MAFLSFAACLFFFFTNVLAHVDHIRFNVVVSLDGSGDFKSISEAIAAAPNNSNTRFYIRV 60
           MAF    +C F  F N LA VD   FN  V+LDG+G+FKSI++AIAAAPNNSNTRFYIRV
Sbjct: 1   MAFFQRISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV 60

Query: 61  SPGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVVNGDHFMAQHL 120
           SPG Y  HLEI   KKFIALIG+N  TTIIVDNRS+  GF T++SAT+VV G++FMAQHL
Sbjct: 61  SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL 120

Query: 121 SFENSAEHQNGQVVAVLNHAKFTTYYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFG 180
           SFENSA+HQ+GQ VAVL+ AK+T YYKCRFLSFQDTLY KG YQFF+ CDIYGTVDFIFG
Sbjct: 121 SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG 180

Query: 181 NGLAMFQDCNIYARLRSNEITVTAQSKEQSWVKSGFSFQNCTVTVSPNIASRKADVKIFL 240
           NGLAMFQDCN+YARL SN+ TVTAQSK+Q W +SGFSFQNCTVTVSP IAS KA VKI+L
Sbjct: 181 NGLAMFQDCNVYARLPSNDSTVTAQSKKQ-WTQSGFSFQNCTVTVSPEIASCKALVKIYL 240

Query: 241 GRPWRQYSTVVFMESFLDDNVQPEGWVLWEGVPINNLFYAEFNNRGPGADTTHRVNWTSF 300
           GRPW+ YSTVVFMESFLDDNVQP+GW+LW GVP+NNLFYAE+NNRGPGA+TTHRVNW  F
Sbjct: 241 GRPWKPYSTVVFMESFLDDNVQPQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPGF 300

Query: 301 HVLDKQLAKNFTIENFINGSDWLPETH 327
           HV+D+QLAK+FT+ENFING+ WLPET+
Sbjct: 301 HVIDRQLAKSFTVENFINGTYWLPETN 326

BLAST of Csor.00g280820 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 279.6 bits (714), Expect = 3.2e-75
Identity = 132/313 (42.17%), Postives = 202/313 (64.54%), Query Frame = 0

Query: 28  VVVSLDGSGDFKSISEAIAAAPNNSNTR---FYIRVSPGTYRGHLEIPPNKKFIALIGDN 87
           V VS DG+G+F +I+ A+AAAPNN++     F I V+ G Y  ++ I  NK+++ +IGD 
Sbjct: 265 VTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDG 324

Query: 88  ALTTIIVDNRSHGTGFSTTDSATLVVNGDHFMAQHLSFENSAEHQNGQVVAVLNHAKFTT 147
              T++  NRS   G++T +SAT  V   +F+A +++F N+A  +  Q VA+ + A F+ 
Sbjct: 325 INQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSI 384

Query: 148 YYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFGNGLAMFQDCNIYAR--LRSNEITV 207
           +Y C F ++QDTLY     QF+RECD+YGTVDFIFGN   +FQ+CN+Y R  + +    +
Sbjct: 385 FYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAI 444

Query: 208 TAQSKEQSWVKSGFSFQNCTVTVSPNIASRKADVKIFLGRPWRQYSTVVFMESFLDDNVQ 267
           TAQ +      +G S QNCT+  + ++ S    VK +LGRPW++YS  V+M+S++D  V+
Sbjct: 445 TAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVE 504

Query: 268 PEGWVLWEG-VPINNLFYAEFNNRGPGADTTHRVNWTSFHVLDKQLAKNFTIENFINGSD 327
           P GW  W G   ++ L+YAE+NN GPG++TT+RV W  +HV++   A NFT+      +D
Sbjct: 505 PVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANFTVTGLFIEAD 564

Query: 328 WLPETHVPFRTGL 335
           W+ +T VP+ +GL
Sbjct: 565 WIWKTGVPYTSGL 577

BLAST of Csor.00g280820 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 278.5 bits (711), Expect = 7.2e-75
Identity = 133/315 (42.22%), Postives = 201/315 (63.81%), Query Frame = 0

Query: 28  VVVSLDGSGDFKSISEAIAAAPN---NSNTRFYIRVSPGTYRGHLEIPPNKKFIALIGDN 87
           V V  +G+G+F +I+ AIAAAPN    SN  F I V+ G Y  ++E+P NK+++ +IGD 
Sbjct: 246 VTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDG 305

Query: 88  ALTTIIVDNRSHGTGFSTTDSATLVVNGDHFMAQHLSFENSAEHQNGQVVAVLNHAKFTT 147
              T+I  NRS   G++T +SAT +++G +F+  +++  N+A    GQ VA+ +    + 
Sbjct: 306 INQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSV 365

Query: 148 YYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFGNGLAMFQDCNIYARL----RSNEI 207
           +Y C F ++QDTLY     QF+RECD+YGTVDFIFGN   + Q+CN+Y R     +SNE 
Sbjct: 366 FYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNE- 425

Query: 208 TVTAQSKEQSWVKSGFSFQNCTVTVSPNIASRKADVKIFLGRPWRQYSTVVFMESFLDDN 267
            VTAQ +      +G +   CT+  + ++A+    VK +LGRPW++YS  V M++++D  
Sbjct: 426 -VTAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGF 485

Query: 268 VQPEGWVLWEG-VPINNLFYAEFNNRGPGADTTHRVNWTSFHVLDKQLAKNFTIENFING 327
           ++P GW  W G   ++ L+YAE+NN GPG+DTT+RV W  +HV++   A NFT+ NF+ G
Sbjct: 486 LEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVG 545

Query: 328 SDWLPETHVPFRTGL 335
             W+ +T VPF  GL
Sbjct: 546 EGWIGQTGVPFVGGL 558

BLAST of Csor.00g280820 vs. TAIR 10
Match: AT2G26440.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 275.8 bits (704), Expect = 4.6e-74
Identity = 136/311 (43.73%), Postives = 194/311 (62.38%), Query Frame = 0

Query: 27  NVVVSLDGSGDFKSISEAIAAAPNNSNTRFYIRVSPGTYRGHLEIPPNKKFIALIGDNAL 86
           ++VV+ DG+G+F +I+EAI+ APN SN R  I V  G Y  +++IP  K  I LIGD + 
Sbjct: 237 SLVVAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDGSD 296

Query: 87  TTIIVDNRSHGTGFSTTDSATLVVNGDHFMAQHLSFENSAEHQNGQVVAVLNHAKFTTYY 146
            T I  NRS G G++T  SATL V+G+ F+A+ +   N+A  +  Q VA+  +A F   Y
Sbjct: 297 VTFITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVALY 356

Query: 147 KCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFGNGLAMFQDCNIYARL-RSNEITV-TA 206
           +C    +QDTLY     QF+RECDIYGT+D+IFGN   +FQ CNI ++L    + TV TA
Sbjct: 357 RCVIDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVITA 416

Query: 207 QSKEQSWVKSGFSFQNCTVTVSPNIASRKADVKIFLGRPWRQYSTVVFMESFLDDNVQPE 266
           QS++     +G S QNC++  S ++ +    VK +LGRPWR++S  V MES++D+ +   
Sbjct: 417 QSRDTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDEFIDGS 476

Query: 267 GWVLWE-GVPINNLFYAEFNNRGPGADTTHRVNWTSFHVLDKQLAKNFTIENFINGSDWL 326
           GW  W  G  ++ L+Y E+NN GPG++T  RVNW  FH++  + A NFT   FI G  WL
Sbjct: 477 GWSKWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIMGYEDAFNFTATEFITGDGWL 536

Query: 327 PETHVPFRTGL 335
             T  P+  G+
Sbjct: 537 GSTSFPYDNGI 547

BLAST of Csor.00g280820 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 275.4 bits (703), Expect = 6.1e-74
Identity = 133/313 (42.49%), Postives = 197/313 (62.94%), Query Frame = 0

Query: 28  VVVSLDGSGDFKSISEAIAAAPNNSNTR---FYIRVSPGTYRGHLEIPPNKKFIALIGDN 87
           V+V    S +F +I++AIAAAPNN+      F I    G Y  ++ +P NKK + L+GD 
Sbjct: 303 VIVGPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDG 362

Query: 88  ALTTIIVDNRSHGTGFSTTDSATLVVNGDHFMAQHLSFENSAEHQNGQVVAVLNHAKFTT 147
              TII  N +   G++T + ++  V G+ FMA  ++F N+A  +  Q VA+ N+A+ ++
Sbjct: 363 INKTIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSS 422

Query: 148 YYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFGNGLAMFQDCNIYAR--LRSNEITV 207
           +Y+C F  +QDTLY+    QF+RECDIYGTVDFIFGN  A+FQ+CNIYAR  +   +  +
Sbjct: 423 FYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAI 482

Query: 208 TAQSKEQSWVKSGFSFQNCTVTVSPNIASRKADVKIFLGRPWRQYSTVVFMESFLDDNVQ 267
           TA  +      +G S  NCT+  +P++A+       FLGRPW+ YS  VFM+S++ D VQ
Sbjct: 483 TAHGRLDPNQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQ 542

Query: 268 PEGWVLWEG-VPINNLFYAEFNNRGPGADTTHRVNWTSFHVLDKQLAKNFTIENFINGSD 327
           P GW+ W G + ++ ++Y E++N GPGA+T  RV W  +++L+   A NFT+ NF  G  
Sbjct: 543 PVGWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDT 602

Query: 328 WLPETHVPFRTGL 335
           WLP+T +PF  GL
Sbjct: 603 WLPQTDIPFYGGL 615

BLAST of Csor.00g280820 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 275.0 bits (702), Expect = 7.9e-74
Identity = 129/313 (41.21%), Postives = 201/313 (64.22%), Query Frame = 0

Query: 28  VVVSLDGSGDFKSISEAIAAAPNNSNTR---FYIRVSPGTYRGHLEIPPNKKFIALIGDN 87
           V V+ +G+G+F +I+EA+ +APN ++     F I V+ G Y  ++ I  NK+++ +IGD 
Sbjct: 259 VTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKRYLMMIGDG 318

Query: 88  ALTTIIVDNRSHGTGFSTTDSATLVVNGDHFMAQHLSFENSAEHQNGQVVAVLNHAKFTT 147
              T++  NR+   G++T +SAT  V   +F+A +++F N+A  +  Q VA+ + A  + 
Sbjct: 319 INRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAMRSSADLSI 378

Query: 148 YYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFGNGLAMFQDCNIYAR--LRSNEITV 207
           +Y C F ++QDTLY     QF+RECDIYGTVDFIFGN   +FQDCN+Y R  +++    +
Sbjct: 379 FYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPMQNQFNAI 438

Query: 208 TAQSKEQSWVKSGFSFQNCTVTVSPNIASRKADVKIFLGRPWRQYSTVVFMESFLDDNVQ 267
           TAQ +      +G S  NCT+  + ++ S    VK +LGRPW++YS  VFM+S++D+ V+
Sbjct: 439 TAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVE 498

Query: 268 PEGWVLWEG-VPINNLFYAEFNNRGPGADTTHRVNWTSFHVLDKQLAKNFTIENFINGSD 327
           P GW  W G   ++ L+YAE+NN G G+ TT RV W  +HV++   A NFT+ENF+ G  
Sbjct: 499 PVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFTVENFLLGDG 558

Query: 328 WLPETHVPFRTGL 335
           W+ ++ VP+ +GL
Sbjct: 559 WMVQSGVPYISGL 571

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O048871.6e-7444.09Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1[more]
Q9SRX44.5e-7442.17Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37... [more]
O222561.0e-7342.22Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3... [more]
O487116.5e-7343.73Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3... [more]
Q94CB18.5e-7342.49Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
KAG6572379.16.49e-252100.00Pectinesterase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022952744.15.74e-24696.73pectinesterase 2-like [Cucurbita moschata][more]
XP_023511444.11.10e-21887.50probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita pepo subsp. pepo][more]
XP_023511662.18.65e-21686.87probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita pepo subsp. pepo][more]
KAG6572378.11.43e-21486.61putative pectinesterase/pectinesterase inhibitor 7, partial [Cucurbita argyrospe... [more]
Match NameE-valueIdentityDescription
A0A6J1GMM02.78e-24696.73Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455349 PE=4 SV=1[more]
A0A6J1GM543.29e-21385.42Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455508 PE=4 SV=1[more]
A0A6J1GK923.36e-18575.15Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455081 PE=4 SV=1[more]
A0A6J1HZD21.63e-18478.51Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111468060 PE=4 SV=1[more]
A0A6J1GLJ03.24e-18175.54Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455081 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G02810.13.2e-7542.17Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G47550.17.2e-7542.22Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G26440.14.6e-7443.73Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G10720.26.1e-7442.49Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02330.17.9e-7441.21Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 27..319
e-value: 3.4E-99
score: 331.3
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 21..334
e-value: 5.6E-110
score: 369.7
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 15..333
NoneNo IPR availablePANTHERPTHR31707:SF231PECTINESTERASE/PECTINESTERASE INHIBITOR 47-RELATEDcoord: 15..333
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 171..180
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 26..332

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g280820.m01Csor.00g280820.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity