Csor.00g280510 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g280510
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionLipoyl synthase, mitochondrial
LocationCsor_Chr19: 10631808 .. 10633864 (+)
RNA-Seq ExpressionCsor.00g280510
SyntenyCsor.00g280510
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGATTTGGCGGGAAAACGCTTTACCAACGAACGTCCTTCATCTGTCCTGAGCTTCTCTACGGGGTCGCCAATCTCACGATGAACAGCCATGGCAGAAGCTTAGCTCGACTCCTAGAATCCGTTGGCAGATCCTTCTCTTCTTCATCTTCCTCAACCGCCGCCTCTGCAACCTCCTCCCAATTCTCTCGAACTTTGGCCGGTCTCCGTGAACGACTCGCCGTTGAGACCCCCTCACTTTCCGACTTCCTTGACCTGCAATCTTCTGAGTCGTATTCGGTGGAGGTTGGTACGAAGAAGAAGCCACTTCCCAAGCCTAAATGGATGAAGGAATCCGTGCCTGGTGGCGAGAAGTATGTTCAAATCAAGAAGAAGCTCCGCGAATTGAAACTTCATACGGTTTGTGAGGAGGCTAGGTGCCCTAATTTGGGGGAATGCTGGTCTGGTGGAGAAACCGGCACTGCCACTGCTACGATTATGATACTTGGTGACACTTGTACCCGTGGTTGCAGGCTAGTGTTAATTCAATTTATTCATTCCATTTCGTTTGACTATTTAATTACCAATGTGTTTTTGTCATATAAATGGCCGTGTTGATTACTTTGATATCATCTCAGTGCCTCAGAACTTCTATTCATTCCATCGCTGTTTTGGGTTTGTTTGTAGTCCAGGTTCTGTAACGTCAAAACATCACGAACTCCGCCTCCACCAGACCCAAATGAGCCGAAAAATGTTGCTGAGGCAATCGCATCATGGGGCTTGGATTATGTGGTCATCACTAGTGTGGATCGCGACGATTTACCTGATCAAGGGAGTGGTCATTTTGCAGAGACTGTGCAGAAACTTAAGGTGTTGAAGCCAAATATGCTGATCGAGGCATTGGGTACTAATTCCCTAGATTCCTTTTCGTATCACAATGTCACTTTCATTGTTGCACTTAAGGATCCTTGACGTGACGCTTGATTTTTACGAGCCTTCTTTTATCTGGTTTCTATCAAACTAAGGAATTAAATGAAAGCCACTTCCATTGTTACATTTTTTTTTTTTTTTTTTTTTTGATGTTGCTTAACTAATTGATTTTGCAGTTCCTGATTTTCGAGGAGACCCCGGCTGTGTAGAAAATGTTGCAAAGTCAGGCCTCGACGTCTTTGCTCATAATATAGAGACAGTTGAGGAGCTTCAGGGAGCTGTTAGGGATCATCGTGCTAATTTTAAGCAATCATTAGATGTTCTAGTTATGGCCAAAGAGTTTGCCCCTTCTGGAACACTTACGAAGACTTCCATTATGTTAGGCTGTGGGGAGACACCGGATCAAGTCGTGCAAACAATGGAGAAGGTGAGAGCAGCTGGAGTTGATGTGATGACGTTTGGTCAGTACATGAGACCTTCAAAGCGCCATATGCCTGTATCAGAATACATCACTCCTGAGGCTTTCGAGAAGTATCAAAATCTTGGCATGAAAATGGTACAGTCTCTTTCTTATTATTTGAACATTTTGGTTGTGTTCTGTCTTTGTTCTGCAAGAGATTGTTTAATCAAGCCCTTAAACCCTTTTTAAATCCCGCTTAAAAACACTTTTTTTTCTTTCACCTAAACAATTTTAAGCCCTCAGATAATCAATCCAATCAGGCTCGTAATCTGGTACGCTGCGGATAATTTTCATTTGCATTTCTTATATGTTCCTTCTCTAGGATTTGTATCGTTGTAATTTTATGTGTAATTGTGTTTTGATTGTTCGAGGGAGTCCATGTTTTAGCTTAAAGTATATACTAGTTCACACTGTTCTGCAGAGCCCTCATTTCTAAATTTATAGGAAATCTCCACCATCAAGTTATCACATGATCAATAGTTTTTTAATGGATGAGGAGTTTGTTGCCATGTCTTGAGGGTGCAAGAATTATCTGAAAAATCCATTTTTTGTTTGGTTGTGACAGGGTTTTCGGTATGTGGCATCTGGCCCAATGGTTAGGTCCTCATACAAAGCAGGAGAATTCTATATCAAATCAATGATTGAATCAGATCGGTCAGCTTCTAGTTCACACCCTACCCAGCCTTGA

mRNA sequence

ATGGGATTTGGCGGGAAAACGCTTTACCAACGAACGTCCTTCATCTGTCCTGAGCTTCTCTACGGGGTCGCCAATCTCACGATGAACAGCCATGGCAGAAGCTTAGCTCGACTCCTAGAATCCGTTGGCAGATCCTTCTCTTCTTCATCTTCCTCAACCGCCGCCTCTGCAACCTCCTCCCAATTCTCTCGAACTTTGGCCGGTCTCCGTGAACGACTCGCCGTTGAGACCCCCTCACTTTCCGACTTCCTTGACCTGCAATCTTCTGAGTCGTATTCGGTGGAGGTTGGTACGAAGAAGAAGCCACTTCCCAAGCCTAAATGGATGAAGGAATCCGTGCCTGGTGGCGAGAAGTATGTTCAAATCAAGAAGAAGCTCCGCGAATTGAAACTTCATACGGTTTGTGAGGAGGCTAGGTGCCCTAATTTGGGGGAATGCTGGTCTGGTGGAGAAACCGGCACTGCCACTGCTACGATTATGATACTTGGTGACACTTGTACCCGTGGTTGCAGGCTAGTGTTCTGTAACGTCAAAACATCACGAACTCCGCCTCCACCAGACCCAAATGAGCCGAAAAATGTTGCTGAGGCAATCGCATCATGGGGCTTGGATTATGTGGTCATCACTAGTGTGGATCGCGACGATTTACCTGATCAAGGGAGTGGTCATTTTGCAGAGACTGTGCAGAAACTTAAGGTGTTGAAGCCAAATATGCTGATCGAGGCATTGGTTCCTGATTTTCGAGGAGACCCCGGCTGTGTAGAAAATGTTGCAAAGTCAGGCCTCGACGTCTTTGCTCATAATATAGAGACAGTTGAGGAGCTTCAGGGAGCTGTTAGGGATCATCGTGCTAATTTTAAGCAATCATTAGATGTTCTAGTTATGGCCAAAGAGTTTGCCCCTTCTGGAACACTTACGAAGACTTCCATTATGTTAGGCTGTGGGGAGACACCGGATCAAGTCGTGCAAACAATGGAGAAGGTGAGAGCAGCTGGAGTTGATGTGATGACGTTTGGTCAGTACATGAGACCTTCAAAGCGCCATATGCCTGTATCAGAATACATCACTCCTGAGGCTTTCGAGAAGTATCAAAATCTTGGCATGAAAATGGGTTTTCGGTATGTGGCATCTGGCCCAATGGTTAGGTCCTCATACAAAGCAGGAGAATTCTATATCAAATCAATGATTGAATCAGATCGGTCAGCTTCTAGTTCACACCCTACCCAGCCTTGA

Coding sequence (CDS)

ATGGGATTTGGCGGGAAAACGCTTTACCAACGAACGTCCTTCATCTGTCCTGAGCTTCTCTACGGGGTCGCCAATCTCACGATGAACAGCCATGGCAGAAGCTTAGCTCGACTCCTAGAATCCGTTGGCAGATCCTTCTCTTCTTCATCTTCCTCAACCGCCGCCTCTGCAACCTCCTCCCAATTCTCTCGAACTTTGGCCGGTCTCCGTGAACGACTCGCCGTTGAGACCCCCTCACTTTCCGACTTCCTTGACCTGCAATCTTCTGAGTCGTATTCGGTGGAGGTTGGTACGAAGAAGAAGCCACTTCCCAAGCCTAAATGGATGAAGGAATCCGTGCCTGGTGGCGAGAAGTATGTTCAAATCAAGAAGAAGCTCCGCGAATTGAAACTTCATACGGTTTGTGAGGAGGCTAGGTGCCCTAATTTGGGGGAATGCTGGTCTGGTGGAGAAACCGGCACTGCCACTGCTACGATTATGATACTTGGTGACACTTGTACCCGTGGTTGCAGGCTAGTGTTCTGTAACGTCAAAACATCACGAACTCCGCCTCCACCAGACCCAAATGAGCCGAAAAATGTTGCTGAGGCAATCGCATCATGGGGCTTGGATTATGTGGTCATCACTAGTGTGGATCGCGACGATTTACCTGATCAAGGGAGTGGTCATTTTGCAGAGACTGTGCAGAAACTTAAGGTGTTGAAGCCAAATATGCTGATCGAGGCATTGGTTCCTGATTTTCGAGGAGACCCCGGCTGTGTAGAAAATGTTGCAAAGTCAGGCCTCGACGTCTTTGCTCATAATATAGAGACAGTTGAGGAGCTTCAGGGAGCTGTTAGGGATCATCGTGCTAATTTTAAGCAATCATTAGATGTTCTAGTTATGGCCAAAGAGTTTGCCCCTTCTGGAACACTTACGAAGACTTCCATTATGTTAGGCTGTGGGGAGACACCGGATCAAGTCGTGCAAACAATGGAGAAGGTGAGAGCAGCTGGAGTTGATGTGATGACGTTTGGTCAGTACATGAGACCTTCAAAGCGCCATATGCCTGTATCAGAATACATCACTCCTGAGGCTTTCGAGAAGTATCAAAATCTTGGCATGAAAATGGGTTTTCGGTATGTGGCATCTGGCCCAATGGTTAGGTCCTCATACAAAGCAGGAGAATTCTATATCAAATCAATGATTGAATCAGATCGGTCAGCTTCTAGTTCACACCCTACCCAGCCTTGA

Protein sequence

MGFGGKTLYQRTSFICPELLYGVANLTMNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQSSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECWSGGETGTATATIMILGDTCTRGCRLVFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSASSSHPTQP
Homology
BLAST of Csor.00g280510 vs. ExPASy Swiss-Prot
Match: B9H5L9 (Lipoyl synthase, mitochondrial OS=Populus trichocarpa OX=3694 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 6.8e-181
Identity = 317/360 (88.06%), Postives = 336/360 (93.33%), Query Frame = 0

Query: 50  SSSTAASATSS--QFSRTLAGLRERLAVETPSLSDFLDLQSSESYSVEVGTKKKPLPKPK 109
           SSST  S+T    QFS+TLAGLR RLAVE+P+LSDF+ LQS+ +YSVEVGTKKKPLPKPK
Sbjct: 26  SSSTVESSTKQPPQFSQTLAGLRARLAVESPTLSDFIHLQSNNTYSVEVGTKKKPLPKPK 85

Query: 110 WMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECWSGGETGTATATIMILGDTCT 169
           WM+E++PGGEKYVQIKKKLRELKLHTVCEEA+CPNLGECWSGGETGTATATIMILGDTCT
Sbjct: 86  WMREAIPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCT 145

Query: 170 RGCRLVFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAET 229
           RGCR  FCNVKTSRTPPPPDPNEP NVAEAIASWGLDYVVITSVDRDDL DQGSGHFAET
Sbjct: 146 RGCR--FCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLADQGSGHFAET 205

Query: 230 VQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAHNIETVEELQGAVRDHRANFK 289
           V KLK LKPNMLIEALVPDFRGD GCVE VAKSGLDVFAHNIETVEELQ +VRDHRANFK
Sbjct: 206 VHKLKTLKPNMLIEALVPDFRGDRGCVEKVAKSGLDVFAHNIETVEELQSSVRDHRANFK 265

Query: 290 QSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKR 349
           QSLDVL+MAKE+AP GTLTKTSIMLGCGE P+QVV+TMEKVRAAGVDVMTFGQYMRPSKR
Sbjct: 266 QSLDVLMMAKEYAPPGTLTKTSIMLGCGEAPEQVVKTMEKVRAAGVDVMTFGQYMRPSKR 325

Query: 350 HMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSASSSHP 408
           HMPVSEYITP+AFEKY+ LGM+MGFRYVASGPMVRSSYKAGEFYIKSMIESDRS SS  P
Sbjct: 326 HMPVSEYITPDAFEKYKTLGMEMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSVSSQLP 383

BLAST of Csor.00g280510 vs. ExPASy Swiss-Prot
Match: Q3LSN5 (Lipoyl synthase 2, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-2 PE=2 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 4.4e-180
Identity = 315/377 (83.55%), Postives = 344/377 (91.25%), Query Frame = 0

Query: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87
           M S  R++A  L    +  SSSS++T  ++  S+  + LA LR RLA+E+PSLSDF+ L+
Sbjct: 2   MYSRFRTVAGNLNCAAKRLSSSSTTTTTTSAPSELQQNLAALRARLAMESPSLSDFISLK 61

Query: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147
           S  +YSVEVGTKKKPLPKPKWMKES+PGGEKYVQIKKKLRELKLHTVCEEA+CPNLGECW
Sbjct: 62  SDNAYSVEVGTKKKPLPKPKWMKESIPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 121

Query: 148 SGGETGTATATIMILGDTCTRGCRLVFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVV 207
           SGGETGTATATIMILGDTCTRGCR  FCNVKTSRTPPPPDP+EP NVAEAIASWGLDYVV
Sbjct: 122 SGGETGTATATIMILGDTCTRGCR--FCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVV 181

Query: 208 ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAH 267
           ITSVDRDDLPDQGSGHF ETVQKLK LKP+MLIEALVPDFRG+  CVE V+KSGLDVFAH
Sbjct: 182 ITSVDRDDLPDQGSGHFTETVQKLKALKPSMLIEALVPDFRGNAECVEKVSKSGLDVFAH 241

Query: 268 NIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEK 327
           NIETVEELQ AVRDHRANFKQSLDVL+MAKE+AP+GTLTKTSIMLGCGETPDQ+V+TMEK
Sbjct: 242 NIETVEELQSAVRDHRANFKQSLDVLMMAKEYAPAGTLTKTSIMLGCGETPDQIVKTMEK 301

Query: 328 VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKA 387
           VRAAGVDVMTFGQ+MRPSKRHMPVSEYITPEAFEKYQ LGM+MGFRYVASGPMVRSSYKA
Sbjct: 302 VRAAGVDVMTFGQHMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKA 361

Query: 388 GEFYIKSMIESDRSASS 405
           GEFYIKSMI+SDR+ASS
Sbjct: 362 GEFYIKSMIDSDRAASS 376

BLAST of Csor.00g280510 vs. ExPASy Swiss-Prot
Match: Q3LSN4 (Lipoyl synthase 1, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-1 PE=2 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 5.7e-180
Identity = 315/377 (83.55%), Postives = 343/377 (90.98%), Query Frame = 0

Query: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87
           M S  R++A  L    +  SSSS++T  ++  S+  + LA LR RLA+E+PSLSDF+ L+
Sbjct: 2   MYSRFRTVAGNLNCAAKRLSSSSTTTTTTSAPSELQQNLAALRARLAMESPSLSDFISLK 61

Query: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147
           S  +YSVEVGTKKKPLPKPKWMKES+PGGEKYVQIKKKLRELKLHTVCEEA+CPNLGECW
Sbjct: 62  SDNAYSVEVGTKKKPLPKPKWMKESIPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 121

Query: 148 SGGETGTATATIMILGDTCTRGCRLVFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVV 207
           SGGETGTATATIMILGDTCTRGCR  FCNVKTSRTPPPPDP+EP NVAEAIASWGLDYVV
Sbjct: 122 SGGETGTATATIMILGDTCTRGCR--FCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVV 181

Query: 208 ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAH 267
           ITSVDRDDLPDQGSGHF ETVQKLK LKP+ LIEALVPDFRG+  CVE V+KSGLDVFAH
Sbjct: 182 ITSVDRDDLPDQGSGHFTETVQKLKALKPSTLIEALVPDFRGNAECVEKVSKSGLDVFAH 241

Query: 268 NIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEK 327
           NIETVEELQ AVRDHRANFKQSLDVL+MAKE+AP+GTLTKTSIMLGCGETPDQ+V+TMEK
Sbjct: 242 NIETVEELQSAVRDHRANFKQSLDVLMMAKEYAPAGTLTKTSIMLGCGETPDQIVKTMEK 301

Query: 328 VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKA 387
           VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQ LGM+MGFRYVASGPMVRSSYKA
Sbjct: 302 VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKA 361

Query: 388 GEFYIKSMIESDRSASS 405
           GEFYIKSMI+SDR+ASS
Sbjct: 362 GEFYIKSMIDSDRAASS 376

BLAST of Csor.00g280510 vs. ExPASy Swiss-Prot
Match: B7FM45 (Lipoyl synthase, mitochondrial OS=Medicago truncatula OX=3880 GN=LIP1 PE=2 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 8.5e-176
Identity = 307/377 (81.43%), Postives = 342/377 (90.72%), Query Frame = 0

Query: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87
           M S  R++A+ L+S  + FS ++++T  + +SS+F + L  LR RLA E+PSLSDF+ L+
Sbjct: 2   MYSRFRTVAKNLKSTTKPFSFTTATTTTTVSSSEFPQNLTELRARLARESPSLSDFISLK 61

Query: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147
           S+ +YSVEVGTKK PLPKPKWMKES+PGG KYVQIKKKLRELKLHTVCEEA+CPN+GECW
Sbjct: 62  SNNAYSVEVGTKKNPLPKPKWMKESIPGGWKYVQIKKKLRELKLHTVCEEAKCPNMGECW 121

Query: 148 SGGETGTATATIMILGDTCTRGCRLVFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVV 207
           SGGETGTATATIMILGDTCTRGCR  FCNVKTSRTPPPPDP+EP NVAEAIASWGLDYVV
Sbjct: 122 SGGETGTATATIMILGDTCTRGCR--FCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVV 181

Query: 208 ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAH 267
           ITSV RDDLPDQGS HF ETVQKLK+LKP++LIEALVPDFRG+  CVE V+KSGLDVFAH
Sbjct: 182 ITSVGRDDLPDQGSSHFTETVQKLKILKPSILIEALVPDFRGNAECVEKVSKSGLDVFAH 241

Query: 268 NIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEK 327
           NIETVEELQ AVRDHRANF QSLDVL MAK++AP+GTLTKTSIMLGCGETPDQ+V+TMEK
Sbjct: 242 NIETVEELQSAVRDHRANFNQSLDVLRMAKDYAPAGTLTKTSIMLGCGETPDQIVKTMEK 301

Query: 328 VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKA 387
           VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQ LGM+MGFRYVASGPMVRSSYKA
Sbjct: 302 VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKA 361

Query: 388 GEFYIKSMIESDRSASS 405
           GEFYIKSMI+SDR+ SS
Sbjct: 362 GEFYIKSMIDSDRAVSS 376

BLAST of Csor.00g280510 vs. ExPASy Swiss-Prot
Match: A5CB81 (Lipoyl synthase, mitochondrial OS=Vitis vinifera OX=29760 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 615.1 bits (1585), Expect = 5.5e-175
Identity = 305/353 (86.40%), Postives = 328/353 (92.92%), Query Frame = 0

Query: 58  TSSQFSRTLAGLRERLAVETPSLSDFLDLQSSESYSVEVGTKKKPLPKPKWMKESVPGGE 117
           T+ +  +TLA LR RLAVE+PSLSDF+ LQ+S+ YSVEVGTKKKPL KPKWMKES+PGG 
Sbjct: 38  TNPEPPQTLASLRHRLAVESPSLSDFVRLQTSDDYSVEVGTKKKPLSKPKWMKESIPGGA 97

Query: 118 KYVQIKKKLRELKLHTVCEEARCPNLGECWSGGETGTATATIMILGDTCTRGCRLVFCNV 177
           KY QIKKKLR+L LHTVCEEARCPN+GECWSGGETGTATATIMILGDTCTRGCR  FCNV
Sbjct: 98  KYTQIKKKLRQLNLHTVCEEARCPNMGECWSGGETGTATATIMILGDTCTRGCR--FCNV 157

Query: 178 KTSRTPPPPDPNEPKNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPN 237
           KTSRTPPPPDP+EP  VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLK+LKPN
Sbjct: 158 KTSRTPPPPDPDEPSKVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKILKPN 217

Query: 238 MLIEALVPDFRGDPGCVENVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAK 297
           MLIEALVPDFRGDPGCVE V+KSGLDVFAHNIETVEELQ AVRDHRANFKQSL+VL +AK
Sbjct: 218 MLIEALVPDFRGDPGCVEKVSKSGLDVFAHNIETVEELQSAVRDHRANFKQSLEVLKLAK 277

Query: 298 EFAPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITP 357
           E+A +GTLTKTSIMLGCGETPDQVV+TMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITP
Sbjct: 278 EYADAGTLTKTSIMLGCGETPDQVVRTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITP 337

Query: 358 EAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSAS--SSHPT 409
           EAFEKY+ LGM MGFRYVASGPMVRSSYKAGEFYIKSMI++DR+ S  SS P+
Sbjct: 338 EAFEKYRILGMDMGFRYVASGPMVRSSYKAGEFYIKSMIDADRAMSWASSSPS 388

BLAST of Csor.00g280510 vs. NCBI nr
Match: KAG6572348.1 (Lipoyl synthase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 811 bits (2094), Expect = 4.80e-296
Identity = 410/410 (100.00%), Postives = 410/410 (100.00%), Query Frame = 0

Query: 1   MGFGGKTLYQRTSFICPELLYGVANLTMNSHGRSLARLLESVGRSFSSSSSSTAASATSS 60
           MGFGGKTLYQRTSFICPELLYGVANLTMNSHGRSLARLLESVGRSFSSSSSSTAASATSS
Sbjct: 1   MGFGGKTLYQRTSFICPELLYGVANLTMNSHGRSLARLLESVGRSFSSSSSSTAASATSS 60

Query: 61  QFSRTLAGLRERLAVETPSLSDFLDLQSSESYSVEVGTKKKPLPKPKWMKESVPGGEKYV 120
           QFSRTLAGLRERLAVETPSLSDFLDLQSSESYSVEVGTKKKPLPKPKWMKESVPGGEKYV
Sbjct: 61  QFSRTLAGLRERLAVETPSLSDFLDLQSSESYSVEVGTKKKPLPKPKWMKESVPGGEKYV 120

Query: 121 QIKKKLRELKLHTVCEEARCPNLGECWSGGETGTATATIMILGDTCTRGCRLVFCNVKTS 180
           QIKKKLRELKLHTVCEEARCPNLGECWSGGETGTATATIMILGDTCTRGCRLVFCNVKTS
Sbjct: 121 QIKKKLRELKLHTVCEEARCPNLGECWSGGETGTATATIMILGDTCTRGCRLVFCNVKTS 180

Query: 181 RTPPPPDPNEPKNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLI 240
           RTPPPPDPNEPKNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLI
Sbjct: 181 RTPPPPDPNEPKNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLI 240

Query: 241 EALVPDFRGDPGCVENVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFA 300
           EALVPDFRGDPGCVENVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFA
Sbjct: 241 EALVPDFRGDPGCVENVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFA 300

Query: 301 PSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAF 360
           PSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAF
Sbjct: 301 PSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAF 360

Query: 361 EKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSASSSHPTQP 410
           EKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSASSSHPTQP
Sbjct: 361 EKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSASSSHPTQP 410

BLAST of Csor.00g280510 vs. NCBI nr
Match: KAG7011959.1 (Lipoyl synthase, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 804 bits (2076), Expect = 2.76e-293
Identity = 409/411 (99.51%), Postives = 409/411 (99.51%), Query Frame = 0

Query: 1   MGFGGKTLYQRTSFICPELLYGVANLTMNSHGRSLARLLESVGRSFSSSSSSTAASATSS 60
           MGFGGKTLYQRTSFICPELLYGVANLTMNSHGRSLARLLESVGRSFSSSSSSTAASATSS
Sbjct: 1   MGFGGKTLYQRTSFICPELLYGVANLTMNSHGRSLARLLESVGRSFSSSSSSTAASATSS 60

Query: 61  QFSRTLAGLRERLAVETPSLSDFLDLQSSESYSVEVGTKKKPLPKPKWMKESVPGGEKYV 120
           QFSRTLAGLRERLAVETPSLSDFLDLQSSESYSVEVGTKKKPLPKPKWMKESVPGGEKYV
Sbjct: 61  QFSRTLAGLRERLAVETPSLSDFLDLQSSESYSVEVGTKKKPLPKPKWMKESVPGGEKYV 120

Query: 121 QIKKKLRELKLHTVCEEARCPNLGECWSGGETGTATATIMILGDTCTRGCRLV-FCNVKT 180
           QIKKKLRELKLHTVCEEARCPNLGECWSGGETGTATATIMILGDTCTRGCRL  FCNVKT
Sbjct: 121 QIKKKLRELKLHTVCEEARCPNLGECWSGGETGTATATIMILGDTCTRGCRLSRFCNVKT 180

Query: 181 SRTPPPPDPNEPKNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNML 240
           SRTPPPPDPNEPKNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNML
Sbjct: 181 SRTPPPPDPNEPKNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNML 240

Query: 241 IEALVPDFRGDPGCVENVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEF 300
           IEALVPDFRGDPGCVENVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEF
Sbjct: 241 IEALVPDFRGDPGCVENVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEF 300

Query: 301 APSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEA 360
           APSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEA
Sbjct: 301 APSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEA 360

Query: 361 FEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSASSSHPTQP 410
           FEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSASSSHPTQP
Sbjct: 361 FEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSASSSHPTQP 411

BLAST of Csor.00g280510 vs. NCBI nr
Match: XP_022952737.1 (lipoyl synthase 2, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 731 bits (1888), Expect = 3.99e-265
Identity = 375/383 (97.91%), Postives = 376/383 (98.17%), Query Frame = 0

Query: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87
           MNSHGRSLARLLESVGR FSSSSSS AASATSSQF RTLAGLRERLAVETPSLSDF+DLQ
Sbjct: 1   MNSHGRSLARLLESVGRFFSSSSSSAAASATSSQFPRTLAGLRERLAVETPSLSDFIDLQ 60

Query: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147
           SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW
Sbjct: 61  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 120

Query: 148 SGGETGTATATIMILGDTCTRGCRLVFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVV 207
           SGGETGTATATIMILGDTCTR  R  FCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVV
Sbjct: 121 SGGETGTATATIMILGDTCTRVSR--FCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVV 180

Query: 208 ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAH 267
           ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAH
Sbjct: 181 ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAH 240

Query: 268 NIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEK 327
           NIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEK
Sbjct: 241 NIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEK 300

Query: 328 VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKA 387
           VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKA
Sbjct: 301 VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKA 360

Query: 388 GEFYIKSMIESDRSASSSHPTQP 410
           GEFYIKSMIESDRSASSSHPTQP
Sbjct: 361 GEFYIKSMIESDRSASSSHPTQP 381

BLAST of Csor.00g280510 vs. NCBI nr
Match: XP_023554514.1 (lipoyl synthase 2, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 727 bits (1876), Expect = 2.69e-263
Identity = 370/383 (96.61%), Postives = 374/383 (97.65%), Query Frame = 0

Query: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87
           MNSHGRSLARLLES+GRSFSSSSSSTAAS T SQF RTL GLRERLA E+PSLSDF+DLQ
Sbjct: 1   MNSHGRSLARLLESIGRSFSSSSSSTAASETFSQFPRTLTGLRERLAAESPSLSDFIDLQ 60

Query: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147
           SSESYSVEVGTKKKPL KPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW
Sbjct: 61  SSESYSVEVGTKKKPLSKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 120

Query: 148 SGGETGTATATIMILGDTCTRGCRLVFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVV 207
           SGGETGTATATIMILGDTCTRGCR  FCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVV
Sbjct: 121 SGGETGTATATIMILGDTCTRGCR--FCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVV 180

Query: 208 ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAH 267
           ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAH
Sbjct: 181 ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAH 240

Query: 268 NIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEK 327
           NIETVEELQGAVRDHRANFK SLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEK
Sbjct: 241 NIETVEELQGAVRDHRANFKPSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEK 300

Query: 328 VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKA 387
           VRAAGVDV+TFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKA
Sbjct: 301 VRAAGVDVITFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKA 360

Query: 388 GEFYIKSMIESDRSASSSHPTQP 410
           GEFYIKSMIESDRSASSSHPTQP
Sbjct: 361 GEFYIKSMIESDRSASSSHPTQP 381

BLAST of Csor.00g280510 vs. NCBI nr
Match: XP_023554516.1 (lipoyl synthase 2, mitochondrial-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 708 bits (1828), Expect = 4.25e-256
Identity = 360/373 (96.51%), Postives = 364/373 (97.59%), Query Frame = 0

Query: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87
           MNSHGRSLARLLES+GRSFSSSSSSTAAS T SQF RTL GLRERLA E+PSLSDF+DLQ
Sbjct: 1   MNSHGRSLARLLESIGRSFSSSSSSTAASETFSQFPRTLTGLRERLAAESPSLSDFIDLQ 60

Query: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147
           SSESYSVEVGTKKKPL KPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW
Sbjct: 61  SSESYSVEVGTKKKPLSKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 120

Query: 148 SGGETGTATATIMILGDTCTRGCRLVFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVV 207
           SGGETGTATATIMILGDTCTRGCR  FCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVV
Sbjct: 121 SGGETGTATATIMILGDTCTRGCR--FCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVV 180

Query: 208 ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAH 267
           ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAH
Sbjct: 181 ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAH 240

Query: 268 NIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEK 327
           NIETVEELQGAVRDHRANFK SLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEK
Sbjct: 241 NIETVEELQGAVRDHRANFKPSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEK 300

Query: 328 VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKA 387
           VRAAGVDV+TFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKA
Sbjct: 301 VRAAGVDVITFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKA 360

Query: 388 GEFYIKSMIESDR 400
           GEFYIKSMIESDR
Sbjct: 361 GEFYIKSMIESDR 371

BLAST of Csor.00g280510 vs. ExPASy TrEMBL
Match: A0A6J1GL74 (Lipoyl synthase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111455343 PE=3 SV=1)

HSP 1 Score: 731 bits (1888), Expect = 1.93e-265
Identity = 375/383 (97.91%), Postives = 376/383 (98.17%), Query Frame = 0

Query: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87
           MNSHGRSLARLLESVGR FSSSSSS AASATSSQF RTLAGLRERLAVETPSLSDF+DLQ
Sbjct: 1   MNSHGRSLARLLESVGRFFSSSSSSAAASATSSQFPRTLAGLRERLAVETPSLSDFIDLQ 60

Query: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147
           SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW
Sbjct: 61  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 120

Query: 148 SGGETGTATATIMILGDTCTRGCRLVFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVV 207
           SGGETGTATATIMILGDTCTR  R  FCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVV
Sbjct: 121 SGGETGTATATIMILGDTCTRVSR--FCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVV 180

Query: 208 ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAH 267
           ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAH
Sbjct: 181 ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAH 240

Query: 268 NIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEK 327
           NIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEK
Sbjct: 241 NIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEK 300

Query: 328 VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKA 387
           VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKA
Sbjct: 301 VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKA 360

Query: 388 GEFYIKSMIESDRSASSSHPTQP 410
           GEFYIKSMIESDRSASSSHPTQP
Sbjct: 361 GEFYIKSMIESDRSASSSHPTQP 381

BLAST of Csor.00g280510 vs. ExPASy TrEMBL
Match: A0A6J1I045 (Lipoyl synthase, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111468222 PE=3 SV=1)

HSP 1 Score: 705 bits (1820), Expect = 3.16e-255
Identity = 362/383 (94.52%), Postives = 366/383 (95.56%), Query Frame = 0

Query: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87
           MNSHGRSLARLLESVGRSFSSSSSS           RTLAGLRERLA ETPSLSDF+DLQ
Sbjct: 1   MNSHGRSLARLLESVGRSFSSSSSSPX---------RTLAGLRERLAAETPSLSDFIDLQ 60

Query: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147
           SSESYSVEVGTKKKPL KPKW+KESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW
Sbjct: 61  SSESYSVEVGTKKKPLSKPKWLKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 120

Query: 148 SGGETGTATATIMILGDTCTRGCRLVFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVV 207
           SGGETGTATATIMILGDTCTRGCRL  CNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVV
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRL--CNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVV 180

Query: 208 ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAH 267
           ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAH
Sbjct: 181 ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAH 240

Query: 268 NIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEK 327
           NIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTL KTSIMLGCGETPDQVVQ+MEK
Sbjct: 241 NIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLAKTSIMLGCGETPDQVVQSMEK 300

Query: 328 VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKA 387
           VRAAGVDVMTFGQYMR SKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGP+VRSSYKA
Sbjct: 301 VRAAGVDVMTFGQYMRTSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPIVRSSYKA 360

Query: 388 GEFYIKSMIESDRSASSSHPTQP 410
           GEFYIKSMIESDRSASSSHPTQP
Sbjct: 361 GEFYIKSMIESDRSASSSHPTQP 372

BLAST of Csor.00g280510 vs. ExPASy TrEMBL
Match: A0A5A7SKJ1 (Lipoyl synthase, mitochondrial OS=Cucumis melo var. makuwa OX=1194695 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 704 bits (1816), Expect = 1.80e-254
Identity = 358/382 (93.72%), Postives = 370/382 (96.86%), Query Frame = 0

Query: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87
           MN   +SLAR+L+SV RSFSSSSSST+ASATSSQF RTLAGLRERLA E+PSLSDF+DLQ
Sbjct: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60

Query: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147
           SS+SYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEA+CPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 148 SGGETGTATATIMILGDTCTRGCRLVFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVV 207
           SGGETGTATATIMILGDTCTRGCR  FCNVKTSRTPPPPDPNEP NVAEAIASWGLDYVV
Sbjct: 121 SGGETGTATATIMILGDTCTRGCR--FCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVV 180

Query: 208 ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAH 267
           ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGD GCVE VA+SGLDVFAH
Sbjct: 181 ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAH 240

Query: 268 NIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEK 327
           NIETVEELQGAVRDHRANFKQSLDVLVMAKEF+PSGTLTKTSIMLGCGETPDQVVQTMEK
Sbjct: 241 NIETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEK 300

Query: 328 VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKA 387
           VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKA
Sbjct: 301 VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKA 360

Query: 388 GEFYIKSMIESDRSASSSHPTQ 409
           GEFYIKSMIESDR+AS+S PTQ
Sbjct: 361 GEFYIKSMIESDRAASNSQPTQ 380

BLAST of Csor.00g280510 vs. ExPASy TrEMBL
Match: A0A1S4E162 (Lipoyl synthase, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495529 PE=3 SV=1)

HSP 1 Score: 704 bits (1816), Expect = 1.80e-254
Identity = 358/382 (93.72%), Postives = 370/382 (96.86%), Query Frame = 0

Query: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87
           MN   +SLAR+L+SV RSFSSSSSST+ASATSSQF RTLAGLRERLA E+PSLSDF+DLQ
Sbjct: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60

Query: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147
           SS+SYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEA+CPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 148 SGGETGTATATIMILGDTCTRGCRLVFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVV 207
           SGGETGTATATIMILGDTCTRGCR  FCNVKTSRTPPPPDPNEP NVAEAIASWGLDYVV
Sbjct: 121 SGGETGTATATIMILGDTCTRGCR--FCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVV 180

Query: 208 ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAH 267
           ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGD GCVE VA+SGLDVFAH
Sbjct: 181 ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAH 240

Query: 268 NIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEK 327
           NIETVEELQGAVRDHRANFKQSLDVLVMAKEF+PSGTLTKTSIMLGCGETPDQVVQTMEK
Sbjct: 241 NIETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEK 300

Query: 328 VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKA 387
           VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKA
Sbjct: 301 VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKA 360

Query: 388 GEFYIKSMIESDRSASSSHPTQ 409
           GEFYIKSMIESDR+AS+S PTQ
Sbjct: 361 GEFYIKSMIESDRAASNSQPTQ 380

BLAST of Csor.00g280510 vs. ExPASy TrEMBL
Match: A0A6J1ERM2 (Lipoyl synthase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111437162 PE=3 SV=1)

HSP 1 Score: 698 bits (1802), Expect = 2.45e-252
Identity = 356/382 (93.19%), Postives = 367/382 (96.07%), Query Frame = 0

Query: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87
           MN+   SLAR+ ESVGRS S SSSST+ASATSSQ  RTLAGLRERLA E+P+LSDF+ L+
Sbjct: 1   MNARRHSLARIFESVGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVGLK 60

Query: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147
           SS+SYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEA+CPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 148 SGGETGTATATIMILGDTCTRGCRLVFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVV 207
           SGGETGTATATIMILGDTCTRGCR  FCNVKTSRTPPPPDPNEP+NVAEAIASWGLDYVV
Sbjct: 121 SGGETGTATATIMILGDTCTRGCR--FCNVKTSRTPPPPDPNEPQNVAEAIASWGLDYVV 180

Query: 208 ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAH 267
           ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPG VE VAKSGLDVFAH
Sbjct: 181 ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGSVEKVAKSGLDVFAH 240

Query: 268 NIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEK 327
           NIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEK
Sbjct: 241 NIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEK 300

Query: 328 VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKA 387
           VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKY NLGMKMGFRYVASGPMVRSSYKA
Sbjct: 301 VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFRYVASGPMVRSSYKA 360

Query: 388 GEFYIKSMIESDRSASSSHPTQ 409
           GEFYIKSMIESDR+ASSSHPTQ
Sbjct: 361 GEFYIKSMIESDRAASSSHPTQ 380

BLAST of Csor.00g280510 vs. TAIR 10
Match: AT2G20860.1 (lipoic acid synthase 1 )

HSP 1 Score: 608.2 bits (1567), Expect = 4.8e-174
Identity = 307/378 (81.22%), Postives = 336/378 (88.89%), Query Frame = 0

Query: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87
           M+S    L R L    R FSSSS+ T  + T S   ++L  LR RLA E+PSL+DF+   
Sbjct: 1   MHSRSALLYRFLRPASRCFSSSSAVTPVTVTQS--PKSLEALRARLANESPSLTDFI--- 60

Query: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147
             ++YSVEVGTKKKPLPKPKWMKES+PGGE+YVQIKKKLR+LKLHTVCEEA+CPNLGECW
Sbjct: 61  HGDTYSVEVGTKKKPLPKPKWMKESIPGGERYVQIKKKLRDLKLHTVCEEAKCPNLGECW 120

Query: 148 SGGETGTATATIMILGDTCTRGCRLVFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVV 207
           SGGETGTATATIMILGDTCTRGCR  FCNVKTSRTPPPPDPNEP NVAEAIASWG+DYVV
Sbjct: 121 SGGETGTATATIMILGDTCTRGCR--FCNVKTSRTPPPPDPNEPNNVAEAIASWGVDYVV 180

Query: 208 ITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAH 267
           ITSVDRDDLPDQGSGHFAETVQ+LK LKP MLIEALVPDFRGD GCVE V+KSGLDV AH
Sbjct: 181 ITSVDRDDLPDQGSGHFAETVQRLKFLKPEMLIEALVPDFRGDGGCVEKVSKSGLDVLAH 240

Query: 268 NIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEK 327
           NIETVEELQ  VRDHRANFKQSLDVL MAKE+AP+GTLTKTS+MLGCGETPDQVV+TMEK
Sbjct: 241 NIETVEELQSFVRDHRANFKQSLDVLRMAKEYAPAGTLTKTSVMLGCGETPDQVVKTMEK 300

Query: 328 VRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKA 387
           VRAAGVDVMTFGQYMRPSKRHMPV+EY+TP+AFE+Y+ LGM+MGFRYVASGPMVRSSYKA
Sbjct: 301 VRAAGVDVMTFGQYMRPSKRHMPVAEYVTPDAFERYRLLGMEMGFRYVASGPMVRSSYKA 360

Query: 388 GEFYIKSMIESDRSASSS 406
           GE+YIKSMIE+DR AS S
Sbjct: 361 GEYYIKSMIEADRVASPS 371

BLAST of Csor.00g280510 vs. TAIR 10
Match: AT5G08415.1 (Radical SAM superfamily protein )

HSP 1 Score: 377.9 bits (969), Expect = 1.1e-104
Identity = 177/301 (58.80%), Postives = 232/301 (77.08%), Query Frame = 0

Query: 105 KPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECWSGGETGTATATIMILGD 164
           KP W+++  P GE++ ++K+ L  L L+TVCEEA+CPN+GECW+GG  G ATATIM+LGD
Sbjct: 97  KPAWLRQKAPQGERFQEVKESLSRLNLNTVCEEAQCPNIGECWNGGGDGVATATIMVLGD 156

Query: 165 TCTRGCRLVFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVVITSVDRDDLPDQGSGHF 224
           TCTRGCR  FC VKTSR PPPPDP EP+N A+AIASWG+DY+VITSVDRDD+PD GSGHF
Sbjct: 157 TCTRGCR--FCAVKTSRNPPPPDPMEPENTAKAIASWGVDYIVITSVDRDDIPDGGSGHF 216

Query: 225 AETVQKLKVLKPNMLIEALVPDFRGDPGCVENVAKSGLDVFAHNIETVEELQGAVRDHRA 284
           A+TV+ +K  KP+++IE L  DFRGD   V+ +  SGLDVFAHN+ETV+ LQ  VRD RA
Sbjct: 217 AQTVKAMKRHKPDIMIECLTSDFRGDLEAVDTLVHSGLDVFAHNVETVKRLQRLVRDPRA 276

Query: 285 NFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRP 344
            ++QS+ VL  AK   P G +TKTSIMLG GET +++ + M  +RA  VD++T GQY++P
Sbjct: 277 GYEQSMSVLKHAKISKP-GMITKTSIMLGLGETDEELKEAMADLRAIDVDILTLGQYLQP 336

Query: 345 SKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSASS 404
           +  H+ V EY+TPE F+ ++  G  +GFRYVASGP+VRSSY+AGE ++K+M++   S S 
Sbjct: 337 TPLHLTVKEYVTPEKFDFWKTYGESIGFRYVASGPLVRSSYRAGELFVKTMVKESYSKSL 394

Query: 405 S 406
           S
Sbjct: 397 S 394

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B9H5L96.8e-18188.06Lipoyl synthase, mitochondrial OS=Populus trichocarpa OX=3694 GN=LIP1 PE=3 SV=1[more]
Q3LSN54.4e-18083.55Lipoyl synthase 2, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-2 PE=2 SV=1[more]
Q3LSN45.7e-18083.55Lipoyl synthase 1, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-1 PE=2 SV=1[more]
B7FM458.5e-17681.43Lipoyl synthase, mitochondrial OS=Medicago truncatula OX=3880 GN=LIP1 PE=2 SV=1[more]
A5CB815.5e-17586.40Lipoyl synthase, mitochondrial OS=Vitis vinifera OX=29760 GN=LIP1 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAG6572348.14.80e-296100.00Lipoyl synthase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7011959.12.76e-29399.51Lipoyl synthase, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrospe... [more]
XP_022952737.13.99e-26597.91lipoyl synthase 2, mitochondrial-like [Cucurbita moschata][more]
XP_023554514.12.69e-26396.61lipoyl synthase 2, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023554516.14.25e-25696.51lipoyl synthase 2, mitochondrial-like isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1GL741.93e-26597.91Lipoyl synthase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111455343 PE=... [more]
A0A6J1I0453.16e-25594.52Lipoyl synthase, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111468222 PE=3 ... [more]
A0A5A7SKJ11.80e-25493.72Lipoyl synthase, mitochondrial OS=Cucumis melo var. makuwa OX=1194695 GN=LIP1 PE... [more]
A0A1S4E1621.80e-25493.72Lipoyl synthase, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495529 PE=3 SV=1[more]
A0A6J1ERM22.45e-25293.19Lipoyl synthase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111437162 PE=... [more]
Match NameE-valueIdentityDescription
AT2G20860.14.8e-17481.22lipoic acid synthase 1 [more]
AT5G08415.11.1e-10458.80Radical SAM superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006638Elp3/MiaB/NifBSMARTSM00729MiaBcoord: 156..365
e-value: 1.5E-4
score: 28.4
IPR031691Lipoyl synthase, N-terminalPFAMPF16881LIAS_Ncoord: 54..140
e-value: 2.0E-17
score: 63.6
IPR007197Radical SAMPFAMPF04055Radical_SAMcoord: 164..327
e-value: 7.8E-9
score: 36.1
IPR007197Radical SAMPROSITEPS51918RADICAL_SAMcoord: 151..373
score: 27.263542
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 149..377
e-value: 5.6E-11
score: 44.0
IPR003698Lipoyl synthaseTIGRFAMTIGR00510TIGR00510coord: 99..395
e-value: 3.0E-119
score: 395.8
IPR003698Lipoyl synthaseSFLDSFLDF00271lipoyl_synthasecoord: 97..395
e-value: 0.0
score: 427.9
IPR003698Lipoyl synthasePANTHERPTHR10949LIPOYL SYNTHASEcoord: 59..402
IPR003698Lipoyl synthaseHAMAPMF_00206Lipoyl_synthcoord: 105..401
score: 129.550552
NoneNo IPR availableSFLDSFLDG01058lipoyl_synthase_likecoord: 97..395
e-value: 0.0
score: 427.9
NoneNo IPR availablePANTHERPTHR10949:SF0LIPOYL SYNTHASE, MITOCHONDRIALcoord: 59..402
NoneNo IPR availableSUPERFAMILY102114Radical SAM enzymescoord: 121..399
IPR027527Lipoyl synthase, mitochondrialHAMAPMF_03128Lipoyl_synth_plantMcoord: 47..404
score: 61.130802

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g280510.m01Csor.00g280510.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009107 lipoate biosynthetic process
biological_process GO:0009249 protein lipoylation
cellular_component GO:0005739 mitochondrion
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0016992 lipoate synthase activity
molecular_function GO:0102552 lipoyl synthase activity (acting on glycine-cleavage complex H protein
molecular_function GO:0102553 lipoyl synthase activity (acting on pyruvate dehydrogenase E2 protein)
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0051536 iron-sulfur cluster binding