Csor.00g277020 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g277020
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Csor_Chr19: 8764543 .. 8765758 (-)
RNA-Seq Expression	Csor.00g277020
Synteny	Csor.00g277020

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGAGTTTGATCAGGCCTTCATCCAAGCCCCGGAGCACCGCCCCAAACTCTCCGCCGCTGAATCCGACGGCATCCCCACCATCGATCTCTCTCCGATCTTCAACTCCCCCGAGGGTACCTTCCCCGACGATTTGGTCCGACAAATTGCCTCCGCTTCCATAGACTGGGGATTCTTTCTTGTGGTCAATCACGGTGTCCCCGCCGAGAAGCGGCGGCGGATGGAGGCCGCTGCTCGGGAGTTTTTCGGGCGGAGTTTGGAAGAGAAGAGAACAGTGATGAAAGTGGAAGGGATGGTCACTGGCTATTCTGATATGGAGCTCACCAAGAACGTTAGAGATTGGAAGGAGGTCTTTGATTTCATTGTGGCCGATCCCACCGTCGTCCCCATGTCGCCCGAGCCTGATGATTATCGGTTGACTCATTGGACCAATCGATGGCCGACATACCTGCCGGAATTCAGGTAACCTGGGTAAAACCCTAATGCTCACCCGAGTCAATTATTAATGAAACTTTTGAAAAATTAAGAGTATTAAATTTATAATTTAACCGTCGTGGTTTGACATGATTGAGTAGAGAGGCTGGTAAGGAATATGCGGAAGAATTAGAGAAACTTGGTCACAAGTTAGTAGAATTGATCGCGGTTAGCTTGGGATTACCGGCACAAAGGTTTCGAGATTATTTCAAAGAGAAAACAAGTATAGTGAGACTGAATCATTATCCTCCATGCTCGTTGCCAGAAATGGCTCTCGGTGTGGGTCGCCACAATGACCCTGGTGTCTTGACGATGCTTGCCCAAGACGATGTCGGAGGGCTAGAAGTGAAAAGGAAAAGAGATGGAGAGTGGATTCGGGTCAAACCCGTACCCGATTCTTACGTCGTCAATGTTGGCAATGTTACTCAGGTATATAGTAGAGCTCGTTGTTGATCAACTGATATGATTGAAACGTTTTTGAAACGATCTTCAACGTATAGGTTTGGAGCAATGAGAGGTACGAGAGCGTGGAGCATAGGGTTATGGTAAACGACAAAAAGGAAAGATATTCCATTGCGTTCTTCTTCAACCCATCTCATTCCACCATTGTCGAACCTTTGGAAGAACTCATCGATCCCGAAAACCCTCCGAAATACCAATCGTACTTATGGGGGAAGCTTCTTTTTAATAAATGTGCAAGGCTTTCAAGAGACTTAAAGCTGCAATATTCAAGTCTCTAA

mRNA sequence

ATGAAGGAGTTTGATCAGGCCTTCATCCAAGCCCCGGAGCACCGCCCCAAACTCTCCGCCGCTGAATCCGACGGCATCCCCACCATCGATCTCTCTCCGATCTTCAACTCCCCCGAGGGTACCTTCCCCGACGATTTGGTCCGACAAATTGCCTCCGCTTCCATAGACTGGGGATTCTTTCTTGTGGTCAATCACGGTGTCCCCGCCGAGAAGCGGCGGCGGATGGAGGCCGCTGCTCGGGAGTTTTTCGGGCGGAGTTTGGAAGAGAAGAGAACAGTGATGAAAGTGGAAGGGATGGTCACTGGCTATTCTGATATGGAGCTCACCAAGAACGTTAGAGATTGGAAGGAGGTCTTTGATTTCATTGTGGCCGATCCCACCGTCGTCCCCATGTCGCCCGAGCCTGATGATTATCGGTTGACTCATTGGACCAATCGATGGCCGACATACCTGCCGGAATTCAGAGAGGCTGGTAAGGAATATGCGGAAGAATTAGAGAAACTTGGTCACAAGTTAGTAGAATTGATCGCGGTTAGCTTGGGATTACCGGCACAAAGGTTTCGAGATTATTTCAAAGAGAAAACAAGTATAGTGAGACTGAATCATTATCCTCCATGCTCGTTGCCAGAAATGGCTCTCGGTGTGGGTCGCCACAATGACCCTGGTGTCTTGACGATGCTTGCCCAAGACGATGTCGGAGGGCTAGAAGTGAAAAGGAAAAGAGATGGAGAGTGGATTCGGGTCAAACCCGTACCCGATTCTTACGTCGTCAATGTTGGCAATGTTACTCAGGTTTGGAGCAATGAGAGGTACGAGAGCGTGGAGCATAGGGTTATGGTAAACGACAAAAAGGAAAGATATTCCATTGCGTTCTTCTTCAACCCATCTCATTCCACCATTGTCGAACCTTTGGAAGAACTCATCGATCCCGAAAACCCTCCGAAATACCAATCGTACTTATGGGGGAAGCTTCTTTTTAATAAATGTGCAAGGCTTTCAAGAGACTTAAAGCTGCAATATTCAAGTCTCTAA

Coding sequence (CDS)

ATGAAGGAGTTTGATCAGGCCTTCATCCAAGCCCCGGAGCACCGCCCCAAACTCTCCGCCGCTGAATCCGACGGCATCCCCACCATCGATCTCTCTCCGATCTTCAACTCCCCCGAGGGTACCTTCCCCGACGATTTGGTCCGACAAATTGCCTCCGCTTCCATAGACTGGGGATTCTTTCTTGTGGTCAATCACGGTGTCCCCGCCGAGAAGCGGCGGCGGATGGAGGCCGCTGCTCGGGAGTTTTTCGGGCGGAGTTTGGAAGAGAAGAGAACAGTGATGAAAGTGGAAGGGATGGTCACTGGCTATTCTGATATGGAGCTCACCAAGAACGTTAGAGATTGGAAGGAGGTCTTTGATTTCATTGTGGCCGATCCCACCGTCGTCCCCATGTCGCCCGAGCCTGATGATTATCGGTTGACTCATTGGACCAATCGATGGCCGACATACCTGCCGGAATTCAGAGAGGCTGGTAAGGAATATGCGGAAGAATTAGAGAAACTTGGTCACAAGTTAGTAGAATTGATCGCGGTTAGCTTGGGATTACCGGCACAAAGGTTTCGAGATTATTTCAAAGAGAAAACAAGTATAGTGAGACTGAATCATTATCCTCCATGCTCGTTGCCAGAAATGGCTCTCGGTGTGGGTCGCCACAATGACCCTGGTGTCTTGACGATGCTTGCCCAAGACGATGTCGGAGGGCTAGAAGTGAAAAGGAAAAGAGATGGAGAGTGGATTCGGGTCAAACCCGTACCCGATTCTTACGTCGTCAATGTTGGCAATGTTACTCAGGTTTGGAGCAATGAGAGGTACGAGAGCGTGGAGCATAGGGTTATGGTAAACGACAAAAAGGAAAGATATTCCATTGCGTTCTTCTTCAACCCATCTCATTCCACCATTGTCGAACCTTTGGAAGAACTCATCGATCCCGAAAACCCTCCGAAATACCAATCGTACTTATGGGGGAAGCTTCTTTTTAATAAATGTGCAAGGCTTTCAAGAGACTTAAAGCTGCAATATTCAAGTCTCTAA

Protein sequence

MKEFDQAFIQAPEHRPKLSAAESDGIPTIDLSPIFNSPEGTFPDDLVRQIASASIDWGFFLVVNHGVPAEKRRRMEAAAREFFGRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFDFIVADPTVVPMSPEPDDYRLTHWTNRWPTYLPEFREAGKEYAEELEKLGHKLVELIAVSLGLPAQRFRDYFKEKTSIVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRKRDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTIVEPLEELIDPENPPKYQSYLWGKLLFNKCARLSRDLKLQYSSL

Homology

[Close]

BLAST of Csor.00g277020 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 5.0e-52
Identity = 121/316 (38.29%), Postives = 178/316 (56.33%), Query Frame = 0


Query: 8   FIQAPEHRPKLS--AAESDGIPTIDLSPIFNSPEGTFPDDLVRQIASASIDWGFFLVVNH 67
           +I+    RP  +  A  +  IP IDL  +F S EG   D ++ +I+ A   WGFF VVNH
Sbjct: 42  YIKPASLRPTTTEDAPTATNIPIIDLEGLF-SEEGLSDDVIMARISEACRGWGFFQVVNH 101

Query: 68  GVPAEKRRRMEAAAREFFGRSLEEKRTVMKVEGMVTGY-SDMELTKNVR-DWKEVFDFIV 127
           GV  E         REFF   +  K T         GY S + + K    DW + + F+ 
Sbjct: 102 GVKPELMDAARENWREFFHMPVNAKETYSNSPRTYEGYGSRLGVEKGASLDWSDYY-FLH 161

Query: 128 ADPTVVPMSPEPDDYRLTHWTNRWPTYLPEFREAGKEYAEELEKLGHKLVELIAVSLGLP 187
             P           + L  + N+WP++ P  RE   EY EEL KL  +++ +++ +LGL 
Sbjct: 162 LLP-----------HHLKDF-NKWPSFPPTIREVIDEYGEELVKLSGRIMRVLSTNLGLK 221

Query: 188 AQRFRDYFKEKT--SIVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDD-VGGLEVKRK 247
             +F++ F  +   + +R+N+YP C  PE+ALG+  H+DPG +T+L  DD V GL+V  +
Sbjct: 222 EDKFQEAFGGENIGACLRVNYYPKCPRPELALGLSPHSDPGGMTILLPDDQVFGLQV--R 281

Query: 248 RDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI 307
           +D  WI VKP P +++VN+G+  Q+ SN  Y+SVEHRV+VN  KER S+AFF+NP     
Sbjct: 282 KDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEHRVIVNSDKERVSLAFFYNPKSDIP 341

Query: 308 VEPLEELIDPENPPKY 317
           ++PL+EL+   NPP Y
Sbjct: 342 IQPLQELVSTHNPPLY 341

[Close]

BLAST of Csor.00g277020 vs. ExPASy Swiss-Prot
Match: Q39110 (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 196.8 bits (499), Expect = 3.9e-49
Identity = 113/320 (35.31%), Postives = 164/320 (51.25%), Query Frame = 0


Query: 8   FIQAPEHRPKLSAAESDGIPTIDLSPIFNSPEGTFPDDLVRQIASASIDWGFFLVVNHGV 67
           FI   + +P ++  E D +P IDL  + + P  T   D  R I+ A    GFFLVVNHG+
Sbjct: 44  FIWPDDEKPSINVLELD-VPLIDLQNLLSDPSSTL--DASRLISEACKKHGFFLVVNHGI 103

Query: 68  PAEKRRRMEAAAREFFGRSLEEKRTVMKVEGMVTGYSDMELTK--NVRDWKEVFDFIVAD 127
             E           FF   L EK+ V++  G   GY+     +      WKE   F   D
Sbjct: 104 SEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLSFRFCD 163

Query: 128 PTVVPMSPEPDDYRLTHWTNRWPTYLPEFREAGKEYAEELEKLGHKLVELIAVSLGLPAQ 187
              +  S    DY    + +        F +  +EY E +  L  K++EL+ +SLG+   
Sbjct: 164 D--MSRSKSVQDY----FCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRD 223

Query: 188 RFRDYFKEKTSIVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRKRDGEW 247
            FR++F+E  SI+RLN+YPPC  P++ LG G H DP  LT+L QD V GL+V    + +W
Sbjct: 224 YFREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQV--FVENQW 283

Query: 248 IRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTIVEPLE 307
             ++P P ++VVN+G+     SN+RY+S  HR +VN + ER S+AFF  P    +V P  
Sbjct: 284 RSIRPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPR 343

Query: 308 ELIDPENPPKYQSYLWGKLL 326
           EL+D     +Y  + W   L
Sbjct: 344 ELLDSITSRRYPDFTWSMFL 352

[Close]

BLAST of Csor.00g277020 vs. ExPASy Swiss-Prot
Match: O80449 (Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 2.6e-48
Identity = 114/319 (35.74%), Postives = 176/319 (55.17%), Query Frame = 0


Query: 8   FIQAPEHRPKLSAAESDG---IPTIDLSPIFNSPEGTFPDDLVRQIASASIDWGFFLVVN 67
           +++    RP  +  +SD    IP +D++ ++  PEG      +R + SA  +WGFF +VN
Sbjct: 27  YVKPAHQRPVFNTTQSDAGIEIPVLDMNDVWGKPEG------LRLVRSACEEWGFFQMVN 86

Query: 68  HGVPAEKRRRMEAAAREFFGRSLEEKRTVMKVEGMVTGY-SDMELTKNVR-DWKEVFDFI 127
           HGV      R+  A REFF   LEEKR          GY S + + K+ + DW + F F+
Sbjct: 87  HGVTHSLMERVRGAWREFFELPLEEKRKYANSPDTYEGYGSRLGVVKDAKLDWSDYF-FL 146

Query: 128 VADPTVVPMSPEPDDYRLTHWTNRWPTYLPEFREAGKEYAEELEKLGHKLVELIAVSLGL 187
              P+ +  +P           ++WP+  P+ RE  ++Y EE+ KL  +L E ++ SLGL
Sbjct: 147 NYLPSSI-RNP-----------SKWPSQPPKIRELIEKYGEEVRKLCERLTETLSESLGL 206

Query: 188 PAQRFRDYFKEKTSI---VRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDD-VGGLEVK 247
              +          +   +R N YP C  P++ LG+  H+DPG +T+L  D+ V GL+V 
Sbjct: 207 KPNKLMQALGGGDKVGASLRTNFYPKCPQPQLTLGLSSHSDPGGITILLPDEKVAGLQV- 266

Query: 248 RKRDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHS 307
           R+ DG W+ +K VP++ +VN+G+  Q+ SN  Y+SVEH+V+VN   ER S+AFF+NP   
Sbjct: 267 RRGDG-WVTIKSVPNALIVNIGDQLQILSNGIYKSVEHQVIVNSGMERVSLAFFYNPRSD 324

Query: 308 TIVEPLEELIDPENPPKYQ 318
             V P+EEL+    P  Y+
Sbjct: 327 IPVGPIEELVTANRPALYK 324

[Close]

BLAST of Csor.00g277020 vs. ExPASy Swiss-Prot
Match: P0C5H5 (Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. indica OX=39946 GN=20ox2 PE=1 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 2.2e-47
Identity = 115/312 (36.86%), Postives = 163/312 (52.24%), Query Frame = 0


Query: 19  SAAESDGIPTIDLSPIFNSPEGTFPDDLVRQIASASIDWGFFLVVNHGVPAEKRRRMEAA 78
           SAAE D +P +D+  +    +         Q+A+A    GFF V  HGV A   R     
Sbjct: 57  SAAELD-MPVVDVG-VLRDGDAEGLRRAAAQVAAACATHGFFQVSEHGVDAALARAALDG 116

Query: 79  AREFFGRSLEEKRTVMKVEGMVTGYSDMELTK--NVRDWKEVFDFIVADPTVVPMSPEPD 138
           A +FF   L EKR   +V G V+GY+     +  +   WKE   F   D    P+     
Sbjct: 117 ASDFFRLPLAEKRRARRVPGTVSGYTSAHADRFASKLPWKETLSFGFHDRAAAPVVA--- 176

Query: 139 DYRLTHWTNRWPTYLPEFREAGK---EYAEELEKLGHKLVELIAVSLGLPAQRFRDYFKE 198
           DY  +       T  P+F   G+   +Y EE+++L   ++EL+ +SLG+    +R++F +
Sbjct: 177 DYFSS-------TLGPDFAPMGRVYQKYCEEMKELSLTIMELLELSLGVERGYYREFFAD 236

Query: 199 KTSIVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRKRDGEWIRVKPVPD 258
            +SI+R N+YPPC  PE  LG G H DP  LT+L QDDVGGLEV    DGEW  V PVP 
Sbjct: 237 SSSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQDDVGGLEV--LVDGEWRPVSPVPG 296

Query: 259 SYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTIVEPLEELIDPENP 318
           + V+N+G+     SN RY+S  HR +VN ++ER S+AFF  P    +V P      P++ 
Sbjct: 297 AMVINIGDTFMALSNGRYKSCLHRAVVNQRRERRSLAFFLCPREDRVVRPPPSAATPQH- 351

Query: 319 PKYQSYLWGKLL 326
             Y  + W  L+
Sbjct: 357 --YPDFTWADLM 351

[Close]

BLAST of Csor.00g277020 vs. ExPASy Swiss-Prot
Match: Q0JH50 (Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GA20OX2 PE=2 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 2.2e-47
Identity = 115/312 (36.86%), Postives = 163/312 (52.24%), Query Frame = 0


Query: 19  SAAESDGIPTIDLSPIFNSPEGTFPDDLVRQIASASIDWGFFLVVNHGVPAEKRRRMEAA 78
           SAAE D +P +D+  +    +         Q+A+A    GFF V  HGV A   R     
Sbjct: 57  SAAELD-MPVVDVG-VLRDGDAEGLRRAAAQVAAACATHGFFQVSEHGVDAALARAALDG 116

Query: 79  AREFFGRSLEEKRTVMKVEGMVTGYSDMELTK--NVRDWKEVFDFIVADPTVVPMSPEPD 138
           A +FF   L EKR   +V G V+GY+     +  +   WKE   F   D    P+     
Sbjct: 117 ASDFFRLPLAEKRRARRVPGTVSGYTSAHADRFASKLPWKETLSFGFHDRAAAPVVA--- 176

Query: 139 DYRLTHWTNRWPTYLPEFREAGK---EYAEELEKLGHKLVELIAVSLGLPAQRFRDYFKE 198
           DY  +       T  P+F   G+   +Y EE+++L   ++EL+ +SLG+    +R++F +
Sbjct: 177 DYFSS-------TLGPDFAPMGRVYQKYCEEMKELSLTIMELLELSLGVERGYYREFFAD 236

Query: 199 KTSIVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRKRDGEWIRVKPVPD 258
            +SI+R N+YPPC  PE  LG G H DP  LT+L QDDVGGLEV    DGEW  V PVP 
Sbjct: 237 SSSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQDDVGGLEV--LVDGEWRPVSPVPG 296

Query: 259 SYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTIVEPLEELIDPENP 318
           + V+N+G+     SN RY+S  HR +VN ++ER S+AFF  P    +V P      P++ 
Sbjct: 297 AMVINIGDTFMALSNGRYKSCLHRAVVNQRRERRSLAFFLCPREDRVVRPPPSAATPQH- 351

Query: 319 PKYQSYLWGKLL 326
             Y  + W  L+
Sbjct: 357 --YPDFTWADLM 351

[Close]

BLAST of Csor.00g277020 vs. NCBI nr
Match: KAG6571999.1 (putative 2-oxoglutarate-dependent dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 707 bits (1824), Expect = 3.65e-257
Identity = 343/343 (100.00%), Postives = 343/343 (100.00%), Query Frame = 0


Query: 1   MKEFDQAFIQAPEHRPKLSAAESDGIPTIDLSPIFNSPEGTFPDDLVRQIASASIDWGFF 60
           MKEFDQAFIQAPEHRPKLSAAESDGIPTIDLSPIFNSPEGTFPDDLVRQIASASIDWGFF
Sbjct: 1   MKEFDQAFIQAPEHRPKLSAAESDGIPTIDLSPIFNSPEGTFPDDLVRQIASASIDWGFF 60

Query: 61  LVVNHGVPAEKRRRMEAAAREFFGRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFD 120
           LVVNHGVPAEKRRRMEAAAREFFGRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFD
Sbjct: 61  LVVNHGVPAEKRRRMEAAAREFFGRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFD 120

Query: 121 FIVADPTVVPMSPEPDDYRLTHWTNRWPTYLPEFREAGKEYAEELEKLGHKLVELIAVSL 180
           FIVADPTVVPMSPEPDDYRLTHWTNRWPTYLPEFREAGKEYAEELEKLGHKLVELIAVSL
Sbjct: 121 FIVADPTVVPMSPEPDDYRLTHWTNRWPTYLPEFREAGKEYAEELEKLGHKLVELIAVSL 180

Query: 181 GLPAQRFRDYFKEKTSIVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRK 240
           GLPAQRFRDYFKEKTSIVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRK
Sbjct: 181 GLPAQRFRDYFKEKTSIVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRK 240

Query: 241 RDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI 300
           RDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI
Sbjct: 241 RDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI 300

Query: 301 VEPLEELIDPENPPKYQSYLWGKLLFNKCARLSRDLKLQYSSL 343
           VEPLEELIDPENPPKYQSYLWGKLLFNKCARLSRDLKLQYSSL
Sbjct: 301 VEPLEELIDPENPPKYQSYLWGKLLFNKCARLSRDLKLQYSSL 343

[Close]

BLAST of Csor.00g277020 vs. NCBI nr
Match: XP_022952113.1 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 [Cucurbita moschata])

HSP 1 Score: 694 bits (1790), Expect = 5.57e-252
Identity = 334/342 (97.66%), Postives = 340/342 (99.42%), Query Frame = 0


Query: 1   MKEFDQAFIQAPEHRPKLSAAESDGIPTIDLSPIFNSPEGTFPDDLVRQIASASIDWGFF 60
           MKEFDQAFIQAPEHRP LSA ESDGIPTIDLSPIFNSP+GTFPDDLVRQIASASIDWGFF
Sbjct: 1   MKEFDQAFIQAPEHRPNLSAVESDGIPTIDLSPIFNSPDGTFPDDLVRQIASASIDWGFF 60

Query: 61  LVVNHGVPAEKRRRMEAAAREFFGRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFD 120
           LVVNHGVPAEKRRRMEAA+REFFGRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFD
Sbjct: 61  LVVNHGVPAEKRRRMEAASREFFGRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFD 120

Query: 121 FIVADPTVVPMSPEPDDYRLTHWTNRWPTYLPEFREAGKEYAEELEKLGHKLVELIAVSL 180
           FIVADPTVVP+SPEPDD+RLTHWTNRWPTYLP+FREAGKEYAEELEKLGHKLVELIAVSL
Sbjct: 121 FIVADPTVVPVSPEPDDHRLTHWTNRWPTYLPKFREAGKEYAEELEKLGHKLVELIAVSL 180

Query: 181 GLPAQRFRDYFKEKTSIVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRK 240
           GLPAQRFRDYFKEKTS+VRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRK
Sbjct: 181 GLPAQRFRDYFKEKTSMVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRK 240

Query: 241 RDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI 300
           RDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI
Sbjct: 241 RDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI 300

Query: 301 VEPLEELIDPENPPKYQSYLWGKLLFNKCARLSRDLKLQYSS 342
           VEPLEELIDPENPPKYQSYLWGKLLFNKCARLSRDLKLQYSS
Sbjct: 301 VEPLEELIDPENPPKYQSYLWGKLLFNKCARLSRDLKLQYSS 342

[Close]

BLAST of Csor.00g277020 vs. NCBI nr
Match: KAG6571998.1 (putative 2-oxoglutarate-dependent dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 684 bits (1764), Expect = 5.12e-248
Identity = 333/343 (97.08%), Postives = 337/343 (98.25%), Query Frame = 0


Query: 1   MKEFDQAFIQAPEHRPKLSAAESDGIPTIDLSPIFNSPEGTFPDDLVRQIASASIDWGFF 60
           MKEFDQAFIQAPEHRP LSAAESD IPTIDLSPIFNSPEGTFPDDLVRQIASASIDWGFF
Sbjct: 1   MKEFDQAFIQAPEHRPNLSAAESDDIPTIDLSPIFNSPEGTFPDDLVRQIASASIDWGFF 60

Query: 61  LVVNHGVPAEKRRRMEAAAREFFGRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFD 120
           LVVNHGVPAEKRRRMEAA+REFFGRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFD
Sbjct: 61  LVVNHGVPAEKRRRMEAASREFFGRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFD 120

Query: 121 FIVADPTVVPMSPEPDDYRLTHWTNRWPTYLPEFREAGKEYAEELEKLGHKLVELIAVSL 180
           FIVADPTVVP+SPEPDD RLTHWTNRWPTYLPEFREAGKEYAEELEKLGHKLVELIAVSL
Sbjct: 121 FIVADPTVVPVSPEPDDDRLTHWTNRWPTYLPEFREAGKEYAEELEKLGHKLVELIAVSL 180

Query: 181 GLPAQRFRDYFKEKTSIVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRK 240
           GLPAQRFRDYFKEKTS+VRLNHYP CS P+MALGVGRHNDPGVLTMLAQDDV GLEVKRK
Sbjct: 181 GLPAQRFRDYFKEKTSMVRLNHYPLCSSPKMALGVGRHNDPGVLTMLAQDDVEGLEVKRK 240

Query: 241 RDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI 300
           RDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI
Sbjct: 241 RDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI 300

Query: 301 VEPLEELIDPENPPKYQSYLWGKLLFNKCARLSRDLKLQYSSL 343
           VEPLEELIDPENPPKYQSYLWGKLLFNKCARLSRDLKLQYSSL
Sbjct: 301 VEPLEELIDPENPPKYQSYLWGKLLFNKCARLSRDLKLQYSSL 343

[Close]

BLAST of Csor.00g277020 vs. NCBI nr
Match: XP_022953057.1 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 [Cucurbita moschata] >XP_022953058.1 probable 2-oxoglutarate-dependent dioxygenase At5g05600 [Cucurbita moschata])

HSP 1 Score: 682 bits (1760), Expect = 2.08e-247
Identity = 328/343 (95.63%), Postives = 338/343 (98.54%), Query Frame = 0


Query: 1   MKEFDQAFIQAPEHRPKLSAAESDGIPTIDLSPIFNSPEGTFPDDLVRQIASASIDWGFF 60
           MKEFDQAFIQAPEHRP LSA ESDGIPTIDLSPIFNSP+GTFPDDLVRQIASASIDWGFF
Sbjct: 1   MKEFDQAFIQAPEHRPNLSAVESDGIPTIDLSPIFNSPDGTFPDDLVRQIASASIDWGFF 60

Query: 61  LVVNHGVPAEKRRRMEAAAREFFGRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFD 120
           LVVNHGVPA KRRRMEAA+REFFGRSLEEKRTVMKVEG++TGYSDMELTKNVRDWKEVFD
Sbjct: 61  LVVNHGVPAGKRRRMEAASREFFGRSLEEKRTVMKVEGVITGYSDMELTKNVRDWKEVFD 120

Query: 121 FIVADPTVVPMSPEPDDYRLTHWTNRWPTYLPEFREAGKEYAEELEKLGHKLVELIAVSL 180
           FIVADPTVVPMSPEP+D+RLTHWTNRWPT+LPEFREAG EYAEELEKLGHKLVELIAVSL
Sbjct: 121 FIVADPTVVPMSPEPNDHRLTHWTNRWPTFLPEFREAGNEYAEELEKLGHKLVELIAVSL 180

Query: 181 GLPAQRFRDYFKEKTSIVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRK 240
           GLPAQRFRDYFKEKTS+VRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRK
Sbjct: 181 GLPAQRFRDYFKEKTSMVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRK 240

Query: 241 RDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI 300
           RDGEWIRVKP+ DSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI
Sbjct: 241 RDGEWIRVKPILDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI 300

Query: 301 VEPLEELIDPENPPKYQSYLWGKLLFNKCARLSRDLKLQYSSL 343
           VEPLEELIDPENPPKY+SYLWGKLLFNKCARLSRDLKLQYSSL
Sbjct: 301 VEPLEELIDPENPPKYKSYLWGKLLFNKCARLSRDLKLQYSSL 343

[Close]

BLAST of Csor.00g277020 vs. NCBI nr
Match: XP_023554291.1 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 681 bits (1758), Expect = 3.49e-247
Identity = 328/338 (97.04%), Postives = 334/338 (98.82%), Query Frame = 0


Query: 1   MKEFDQAFIQAPEHRPKLSAAESDGIPTIDLSPIFNSPEGTFPDDLVRQIASASIDWGFF 60
           MKEFDQAFIQAPEHRP LSA ESDGIP IDLSPIFNSP+GTFPDDLVRQIASASIDWGFF
Sbjct: 1   MKEFDQAFIQAPEHRPNLSAVESDGIPIIDLSPIFNSPDGTFPDDLVRQIASASIDWGFF 60

Query: 61  LVVNHGVPAEKRRRMEAAAREFFGRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFD 120
           LVVNHGVPAEKRRRMEAA+REFF RSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFD
Sbjct: 61  LVVNHGVPAEKRRRMEAASREFFERSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFD 120

Query: 121 FIVADPTVVPMSPEPDDYRLTHWTNRWPTYLPEFREAGKEYAEELEKLGHKLVELIAVSL 180
           FIVADPTVVP+SPEPDD+RLTHWTNRWPTY+PEFREAGKEYAEELEKLGHKLVELIAVSL
Sbjct: 121 FIVADPTVVPVSPEPDDHRLTHWTNRWPTYMPEFREAGKEYAEELEKLGHKLVELIAVSL 180

Query: 181 GLPAQRFRDYFKEKTSIVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRK 240
           GLPAQRFRDYFKEKTS+VRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRK
Sbjct: 181 GLPAQRFRDYFKEKTSMVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRK 240

Query: 241 RDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI 300
           RDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI
Sbjct: 241 RDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI 300

Query: 301 VEPLEELIDPENPPKYQSYLWGKLLFNKCARLSRDLKL 338
           VEPLEELIDPENPPKYQSYLWGKLLFNKCARLSRDLKL
Sbjct: 301 VEPLEELIDPENPPKYQSYLWGKLLFNKCARLSRDLKL 338

[Close]

BLAST of Csor.00g277020 vs. ExPASy TrEMBL
Match: A0A6J1GJK3 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Cucurbita moschata OX=3662 GN=LOC111454879 PE=3 SV=1)

HSP 1 Score: 694 bits (1790), Expect = 2.69e-252
Identity = 334/342 (97.66%), Postives = 340/342 (99.42%), Query Frame = 0


Query: 1   MKEFDQAFIQAPEHRPKLSAAESDGIPTIDLSPIFNSPEGTFPDDLVRQIASASIDWGFF 60
           MKEFDQAFIQAPEHRP LSA ESDGIPTIDLSPIFNSP+GTFPDDLVRQIASASIDWGFF
Sbjct: 1   MKEFDQAFIQAPEHRPNLSAVESDGIPTIDLSPIFNSPDGTFPDDLVRQIASASIDWGFF 60

Query: 61  LVVNHGVPAEKRRRMEAAAREFFGRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFD 120
           LVVNHGVPAEKRRRMEAA+REFFGRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFD
Sbjct: 61  LVVNHGVPAEKRRRMEAASREFFGRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFD 120

Query: 121 FIVADPTVVPMSPEPDDYRLTHWTNRWPTYLPEFREAGKEYAEELEKLGHKLVELIAVSL 180
           FIVADPTVVP+SPEPDD+RLTHWTNRWPTYLP+FREAGKEYAEELEKLGHKLVELIAVSL
Sbjct: 121 FIVADPTVVPVSPEPDDHRLTHWTNRWPTYLPKFREAGKEYAEELEKLGHKLVELIAVSL 180

Query: 181 GLPAQRFRDYFKEKTSIVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRK 240
           GLPAQRFRDYFKEKTS+VRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRK
Sbjct: 181 GLPAQRFRDYFKEKTSMVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRK 240

Query: 241 RDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI 300
           RDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI
Sbjct: 241 RDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI 300

Query: 301 VEPLEELIDPENPPKYQSYLWGKLLFNKCARLSRDLKLQYSS 342
           VEPLEELIDPENPPKYQSYLWGKLLFNKCARLSRDLKLQYSS
Sbjct: 301 VEPLEELIDPENPPKYQSYLWGKLLFNKCARLSRDLKLQYSS 342

[Close]

BLAST of Csor.00g277020 vs. ExPASy TrEMBL
Match: A0A6J1GMB5 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Cucurbita moschata OX=3662 GN=LOC111455577 PE=3 SV=1)

HSP 1 Score: 682 bits (1760), Expect = 1.01e-247
Identity = 328/343 (95.63%), Postives = 338/343 (98.54%), Query Frame = 0


Query: 1   MKEFDQAFIQAPEHRPKLSAAESDGIPTIDLSPIFNSPEGTFPDDLVRQIASASIDWGFF 60
           MKEFDQAFIQAPEHRP LSA ESDGIPTIDLSPIFNSP+GTFPDDLVRQIASASIDWGFF
Sbjct: 1   MKEFDQAFIQAPEHRPNLSAVESDGIPTIDLSPIFNSPDGTFPDDLVRQIASASIDWGFF 60

Query: 61  LVVNHGVPAEKRRRMEAAAREFFGRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFD 120
           LVVNHGVPA KRRRMEAA+REFFGRSLEEKRTVMKVEG++TGYSDMELTKNVRDWKEVFD
Sbjct: 61  LVVNHGVPAGKRRRMEAASREFFGRSLEEKRTVMKVEGVITGYSDMELTKNVRDWKEVFD 120

Query: 121 FIVADPTVVPMSPEPDDYRLTHWTNRWPTYLPEFREAGKEYAEELEKLGHKLVELIAVSL 180
           FIVADPTVVPMSPEP+D+RLTHWTNRWPT+LPEFREAG EYAEELEKLGHKLVELIAVSL
Sbjct: 121 FIVADPTVVPMSPEPNDHRLTHWTNRWPTFLPEFREAGNEYAEELEKLGHKLVELIAVSL 180

Query: 181 GLPAQRFRDYFKEKTSIVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRK 240
           GLPAQRFRDYFKEKTS+VRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRK
Sbjct: 181 GLPAQRFRDYFKEKTSMVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRK 240

Query: 241 RDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI 300
           RDGEWIRVKP+ DSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI
Sbjct: 241 RDGEWIRVKPILDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI 300

Query: 301 VEPLEELIDPENPPKYQSYLWGKLLFNKCARLSRDLKLQYSSL 343
           VEPLEELIDPENPPKY+SYLWGKLLFNKCARLSRDLKLQYSSL
Sbjct: 301 VEPLEELIDPENPPKYKSYLWGKLLFNKCARLSRDLKLQYSSL 343

[Close]

BLAST of Csor.00g277020 vs. ExPASy TrEMBL
Match: A0A6J1I5S1 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Cucurbita maxima OX=3661 GN=LOC111470932 PE=3 SV=1)

HSP 1 Score: 672 bits (1733), Expect = 1.32e-243
Identity = 322/342 (94.15%), Postives = 335/342 (97.95%), Query Frame = 0


Query: 1   MKEFDQAFIQAPEHRPKLSAAESDGIPTIDLSPIFNSPEGTFPDDLVRQIASASIDWGFF 60
           MKEFDQAFIQAPEHRP LSAAESD IPTIDLSPIFNSP+GTFPDDLVRQIAS SIDWGFF
Sbjct: 1   MKEFDQAFIQAPEHRPNLSAAESDCIPTIDLSPIFNSPDGTFPDDLVRQIASTSIDWGFF 60

Query: 61  LVVNHGVPAEKRRRMEAAAREFFGRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFD 120
           LVVNHGVPAEKR+RMEAA+REFF RSLEEKRTVMK EGMVTGYSDMELTKNVRDWKEVFD
Sbjct: 61  LVVNHGVPAEKRQRMEAASREFFRRSLEEKRTVMKEEGMVTGYSDMELTKNVRDWKEVFD 120

Query: 121 FIVADPTVVPMSPEPDDYRLTHWTNRWPTYLPEFREAGKEYAEELEKLGHKLVELIAVSL 180
           FIVADPTVVP+SPEP+D+RLTHWTN+WPTYLPEFREAGKEYAEELEKLGHKLVELIAVSL
Sbjct: 121 FIVADPTVVPVSPEPNDHRLTHWTNQWPTYLPEFREAGKEYAEELEKLGHKLVELIAVSL 180

Query: 181 GLPAQRFRDYFKEKTSIVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRK 240
           GLPAQRFRDYFKEKTS+VRLNHYPPCSLPEMALGVGRHNDPGVLTML QDDVGGLEVKRK
Sbjct: 181 GLPAQRFRDYFKEKTSMVRLNHYPPCSLPEMALGVGRHNDPGVLTMLVQDDVGGLEVKRK 240

Query: 241 RDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI 300
           RDGEWIRVKPVPDSYVVN+GNVTQVWSN+RYESVEHRV+VNDKKERYSIAFFFNPSHSTI
Sbjct: 241 RDGEWIRVKPVPDSYVVNIGNVTQVWSNDRYESVEHRVVVNDKKERYSIAFFFNPSHSTI 300

Query: 301 VEPLEELIDPENPPKYQSYLWGKLLFNKCARLSRDLKLQYSS 342
           VEPLEELIDP+NPPKYQSYLWGK+L NKCARLSRDLKLQYSS
Sbjct: 301 VEPLEELIDPKNPPKYQSYLWGKVLLNKCARLSRDLKLQYSS 342

[Close]

BLAST of Csor.00g277020 vs. ExPASy TrEMBL
Match: A0A6J1I4K7 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111470930 PE=3 SV=1)

HSP 1 Score: 670 bits (1729), Expect = 5.37e-243
Identity = 319/342 (93.27%), Postives = 335/342 (97.95%), Query Frame = 0


Query: 1   MKEFDQAFIQAPEHRPKLSAAESDGIPTIDLSPIFNSPEGTFPDDLVRQIASASIDWGFF 60
           M +FDQAFIQAPEHRPKLS  E+DGIPT+DLSPIFNSP+GTFPDDLVRQIAS SIDWGFF
Sbjct: 1   MDDFDQAFIQAPEHRPKLSDPEADGIPTVDLSPIFNSPDGTFPDDLVRQIASTSIDWGFF 60

Query: 61  LVVNHGVPAEKRRRMEAAAREFFGRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFD 120
           LVVNHGVPAEKR+RMEAA+REFF RSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFD
Sbjct: 61  LVVNHGVPAEKRQRMEAASREFFRRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFD 120

Query: 121 FIVADPTVVPMSPEPDDYRLTHWTNRWPTYLPEFREAGKEYAEELEKLGHKLVELIAVSL 180
           FIVADPTVVP+SPEP+D+RLTHWTN+WPTYLPEFREAGKEYAEELEKLGHKLVELIAVSL
Sbjct: 121 FIVADPTVVPVSPEPNDHRLTHWTNQWPTYLPEFREAGKEYAEELEKLGHKLVELIAVSL 180

Query: 181 GLPAQRFRDYFKEKTSIVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRK 240
           GLPAQRFRDYFKEKTS+VRLNHYPPCSLPEMALGVGRHNDPGVLTML QDDVGGLEVKRK
Sbjct: 181 GLPAQRFRDYFKEKTSMVRLNHYPPCSLPEMALGVGRHNDPGVLTMLVQDDVGGLEVKRK 240

Query: 241 RDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI 300
           RDGEWIRVKPVPDSYVVN+GNVTQVWSN+RYESVEHRV+VNDKKERYSIAFFFNPSHSTI
Sbjct: 241 RDGEWIRVKPVPDSYVVNIGNVTQVWSNDRYESVEHRVVVNDKKERYSIAFFFNPSHSTI 300

Query: 301 VEPLEELIDPENPPKYQSYLWGKLLFNKCARLSRDLKLQYSS 342
           VEPLEELIDP+NPPKYQSYLWGK+L NKCARLSRDLKLQYSS
Sbjct: 301 VEPLEELIDPKNPPKYQSYLWGKVLLNKCARLSRDLKLQYSS 342

[Close]

BLAST of Csor.00g277020 vs. ExPASy TrEMBL
Match: A0A6J1I5R5 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111470930 PE=3 SV=1)

HSP 1 Score: 628 bits (1620), Expect = 2.85e-226
Identity = 298/328 (90.85%), Postives = 314/328 (95.73%), Query Frame = 0


Query: 1   MKEFDQAFIQAPEHRPKLSAAESDGIPTIDLSPIFNSPEGTFPDDLVRQIASASIDWGFF 60
           M +FDQAFIQAPEHRPKLS  E+DGIPT+DLSPIFNSP+GTFPDDLVRQIAS SIDWGFF
Sbjct: 1   MDDFDQAFIQAPEHRPKLSDPEADGIPTVDLSPIFNSPDGTFPDDLVRQIASTSIDWGFF 60

Query: 61  LVVNHGVPAEKRRRMEAAAREFFGRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFD 120
           LVVNHGVPAEKR+RMEAA+REFF RSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFD
Sbjct: 61  LVVNHGVPAEKRQRMEAASREFFRRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEVFD 120

Query: 121 FIVADPTVVPMSPEPDDYRLTHWTNRWPTYLPEFREAGKEYAEELEKLGHKLVELIAVSL 180
           FIVADPTVVP+SPEP+D+RLTHWTN+WPTYLPEFREAGKEYAEELEKLGHKLVELIAVSL
Sbjct: 121 FIVADPTVVPVSPEPNDHRLTHWTNQWPTYLPEFREAGKEYAEELEKLGHKLVELIAVSL 180

Query: 181 GLPAQRFRDYFKEKTSIVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVKRK 240
           GLPAQRFRDYFKEKTS+VRLNHYPPCSLPEMALGVGRHNDPGVLTML QDDVGGLEVKRK
Sbjct: 181 GLPAQRFRDYFKEKTSMVRLNHYPPCSLPEMALGVGRHNDPGVLTMLVQDDVGGLEVKRK 240

Query: 241 RDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHSTI 300
           RDGEWIRVKPVPDSYVVN+GNVTQ WSNERYESVEHR MVN KKERYSIAFFFNPSHST+
Sbjct: 241 RDGEWIRVKPVPDSYVVNIGNVTQAWSNERYESVEHRAMVNHKKERYSIAFFFNPSHSTV 300

Query: 301 VEPLEELIDPENPPKYQSYLWGKLLFNK 328
           VEPL+ELI PENPP Y+SY WGK L N+
Sbjct: 301 VEPLKELITPENPPNYKSYSWGKYLSNR 328

[Close]

BLAST of Csor.00g277020 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 376.7 bits (966), Expect = 2.0e-104
Identity = 184/336 (54.76%), Postives = 227/336 (67.56%), Query Frame = 0


Query: 1   MKEFDQAFIQAPEHRPKLSAAE------SDGIPTIDLSPIFNSPEGTFPDD--LVRQIAS 60
           M E D+AFIQAPEHRP            SD IPTIDLS    S E T  D   + ++IA 
Sbjct: 1   MGELDEAFIQAPEHRPNTHLTNSGDFIFSDEIPTIDLS----SLEDTHHDKTAIAKEIAE 60

Query: 61  ASIDWGFFLVVNHGVPAEKRRRMEAAAREFFGRSLEEKRTVMKVEGMVTGYSDMELTKNV 120
           A   WGFF V+NHG+P+  R R+E  A EFF  + EEKR V + E    GY D E TKNV
Sbjct: 61  ACKRWGFFQVINHGLPSALRHRVEKTAAEFFNLTTEEKRKVKRDEVNPMGYHDEEHTKNV 120

Query: 121 RDWKEVFDFIVADPTVVPMSPEPDDYRLTHWTNRWPTYLPEFREAGKEYAEELEKLGHKL 180
           RDWKE+FDF + D T+VP SPEP+D  L   TN+WP     FRE  +EYA E+EKL  +L
Sbjct: 121 RDWKEIFDFFLQDSTIVPASPEPEDTELRKLTNQWPQNPSHFREVCQEYAREVEKLAFRL 180

Query: 181 VELIAVSLGLPAQRFRDYFKEKTSIVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDV 240
           +EL+++SLGLP  R   +F E+TS +R NHYPPC  PE+ALGVGRH D G LT+LAQD V
Sbjct: 181 LELVSISLGLPGDRLTGFFNEQTSFLRFNHYPPCPNPELALGVGRHKDGGALTVLAQDSV 240

Query: 241 GGLEVKRKRDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFF 300
           GGL+V R+ DG+WI VKP+ D+ ++N+GN  QVW+N+ Y S EHRV+VN  KER+SI FF
Sbjct: 241 GGLQVSRRSDGQWIPVKPISDALIINMGNCIQVWTNDEYWSAEHRVVVNTSKERFSIPFF 300

Query: 301 FNPSHSTIVEPLEELIDPENPPKYQSYLWGKLLFNK 329
           F PSH   +EPLEELI  ENPP Y+ Y WGK   ++
Sbjct: 301 FFPSHEANIEPLEELISEENPPCYKKYNWGKFFVSR 332

[Close]

BLAST of Csor.00g277020 vs. TAIR 10
Match: AT3G19010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 347.1 bits (889), Expect = 1.7e-95
Identity = 165/341 (48.39%), Postives = 227/341 (66.57%), Query Frame = 0


Query: 1   MKEFDQAFIQAPEHR--PKLSAAESDGIPTIDLSPIFNSPEGTFPDDLVRQIASASIDWG 60
           M++ D  +IQAPEHR        + + IP IDLS + + PE     +++ +I  A   WG
Sbjct: 1   MEDLDPTYIQAPEHRSDSNFIPNQPEEIPVIDLSRL-DDPEDV--QNVISEIGDACEKWG 60

Query: 61  FFLVVNHGVPAEKRRRMEAAAREFFGRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEV 120
           FF V+NHGVP++ R+R+E   + FF   +EEK  V + +    GY D E TKNV+DWKEV
Sbjct: 61  FFQVLNHGVPSDARQRVEKTVKMFFDLPMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEV 120

Query: 121 FDFIVADPTVVPMSPEPDDYRLTHWTNRWPTYLPEFREAGKEYAEELEKLGHKLVELIAV 180
           FD    DP V+P + +P+D  L    N+WP    +FREA + YA   EKL  KL+ELI++
Sbjct: 121 FDIYFKDPMVIPSTTDPEDEGLRLVYNKWPQSPSDFREACEVYARHAEKLAFKLLELISL 180

Query: 181 SLGLPAQRFRDYFKEKTSIVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVK 240
           SLGLP +RF DYFKE+ S  R+N YPPC  P++ALGVG H D  V+++LAQDDVGGL+V 
Sbjct: 181 SLGLPKERFHDYFKEQMSFFRINRYPPCPRPDLALGVGHHKDADVISLLAQDDVGGLQVS 240

Query: 241 RKRDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHS 300
           R+ DG W  ++PVP++ V+N+GN  ++W+N++Y S EHRV+VN  +ERYSI FF  PSH 
Sbjct: 241 RRSDGVWFPIRPVPNALVINIGNCMEIWTNDKYWSAEHRVVVNTTRERYSIPFFLLPSHD 300

Query: 301 TIVEPLEELIDPENPPKYQSYLWGKLLFNKCARLSRDLKLQ 340
             V+PLEEL+ PENPPKY+ Y WGK   ++     R L+++
Sbjct: 301 VEVKPLEELVSPENPPKYKGYKWGKFYVSRNRSDFRKLEIE 338

[Close]

BLAST of Csor.00g277020 vs. TAIR 10
Match: AT3G19000.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 295.4 bits (755), Expect = 5.8e-80
Identity = 147/273 (53.85%), Postives = 182/273 (66.67%), Query Frame = 0


Query: 1   MKEFDQAFIQAPEHRPKLSAAE------SDGIPTIDLSPIFNSPEGTFPDD--LVRQIAS 60
           M E D+AFIQAPEHRP            SD IPTIDLS    S E T  D   + ++IA 
Sbjct: 1   MGELDEAFIQAPEHRPNTHLTNSGDFIFSDEIPTIDLS----SLEDTHHDKTAIAKEIAE 60

Query: 61  ASIDWGFFLVVNHGVPAEKRRRMEAAAREFFGRSLEEKRTVMKVEGMVTGYSDMELTKNV 120
           A   WGFF V+NHG+P+  R R+E  A EFF  + EEKR V + E    GY D E TKNV
Sbjct: 61  ACKRWGFFQVINHGLPSALRHRVEKTAAEFFNLTTEEKRKVKRDEVNPMGYHDEEHTKNV 120

Query: 121 RDWKEVFDFIVADPTVVPMSPEPDDYRLTHWTNRWPTYLPEFREAGKEYAEELEKLGHKL 180
           RDWKE+FDF + D T+VP SPEP+D  L   TN+WP     FRE  +EYA E+EKL  +L
Sbjct: 121 RDWKEIFDFFLQDSTIVPASPEPEDTELRKLTNQWPQNPSHFREVCQEYAREVEKLAFRL 180

Query: 181 VELIAVSLGLPAQRFRDYFKEKTSIVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDV 240
           +EL+++SLGLP  R   +F E+TS +R NHYPPC  PE+ALGVGRH D G LT+LAQD V
Sbjct: 181 LELVSISLGLPGDRLTGFFNEQTSFLRFNHYPPCPNPELALGVGRHKDGGALTVLAQDSV 240

Query: 241 GGLEVKRKRDGEWIRVKPVPDSYVVNVGNVTQV 266
           GGL+V R+ DG+WI VKP+ D+ ++N+GN  QV
Sbjct: 241 GGLQVSRRSDGQWIPVKPISDALIINMGNCIQV 269

[Close]

BLAST of Csor.00g277020 vs. TAIR 10
Match: AT3G19010.3 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 292.0 bits (746), Expect = 6.4e-79
Identity = 146/341 (42.82%), Postives = 203/341 (59.53%), Query Frame = 0


Query: 1   MKEFDQAFIQAPEHR--PKLSAAESDGIPTIDLSPIFNSPEGTFPDDLVRQIASASIDWG 60
           M++ D  +IQAPEHR        + + IP IDLS + + PE     +++ +I  A   WG
Sbjct: 1   MEDLDPTYIQAPEHRSDSNFIPNQPEEIPVIDLSRL-DDPEDV--QNVISEIGDACEKWG 60

Query: 61  FFLVVNHGVPAEKRRRMEAAAREFFGRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEV 120
           FF V+NHGVP++ R+R+E   + FF   +EEK  V + +    GY D E TKNV+DWKEV
Sbjct: 61  FFQVLNHGVPSDARQRVEKTVKMFFDLPMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEV 120

Query: 121 FDFIVADPTVVPMSPEPDDYRLTHWTNRWPTYLPEFREAGKEYAEELEKLGHKLVELIAV 180
           FD    DP V+P + +P+D  L    N+WP    +FREA + YA   EKL          
Sbjct: 121 FDIYFKDPMVIPSTTDPEDEGLRLVYNKWPQSPSDFREACEVYARHAEKLA--------- 180

Query: 181 SLGLPAQRFRDYFKEKTSIVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVK 240
                                +N YPPC  P++ALGVG H D  V+++LAQDDVGGL+V 
Sbjct: 181 ---------------------INRYPPCPRPDLALGVGHHKDADVISLLAQDDVGGLQVS 240

Query: 241 RKRDGEWIRVKPVPDSYVVNVGNVTQVWSNERYESVEHRVMVNDKKERYSIAFFFNPSHS 300
           R+ DG W  ++PVP++ V+N+GN  ++W+N++Y S EHRV+VN  +ERYSI FF  PSH 
Sbjct: 241 RRSDGVWFPIRPVPNALVINIGNCMEIWTNDKYWSAEHRVVVNTTRERYSIPFFLLPSHD 300

Query: 301 TIVEPLEELIDPENPPKYQSYLWGKLLFNKCARLSRDLKLQ 340
             V+PLEEL+ PENPPKY+ Y WGK   ++     R L+++
Sbjct: 301 VEVKPLEELVSPENPPKYKGYKWGKFYVSRNRSDFRKLEIE 308

[Close]

BLAST of Csor.00g277020 vs. TAIR 10
Match: AT3G19010.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 261.9 bits (668), Expect = 7.1e-70
Identity = 127/267 (47.57%), Postives = 175/267 (65.54%), Query Frame = 0


Query: 1   MKEFDQAFIQAPEHR--PKLSAAESDGIPTIDLSPIFNSPEGTFPDDLVRQIASASIDWG 60
           M++ D  +IQAPEHR        + + IP IDLS + + PE     +++ +I  A   WG
Sbjct: 1   MEDLDPTYIQAPEHRSDSNFIPNQPEEIPVIDLSRL-DDPEDV--QNVISEIGDACEKWG 60

Query: 61  FFLVVNHGVPAEKRRRMEAAAREFFGRSLEEKRTVMKVEGMVTGYSDMELTKNVRDWKEV 120
           FF V+NHGVP++ R+R+E   + FF   +EEK  V + +    GY D E TKNV+DWKEV
Sbjct: 61  FFQVLNHGVPSDARQRVEKTVKMFFDLPMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEV 120

Query: 121 FDFIVADPTVVPMSPEPDDYRLTHWTNRWPTYLPEFREAGKEYAEELEKLGHKLVELIAV 180
           FD    DP V+P + +P+D  L    N+WP    +FREA + YA   EKL  KL+ELI++
Sbjct: 121 FDIYFKDPMVIPSTTDPEDEGLRLVYNKWPQSPSDFREACEVYARHAEKLAFKLLELISL 180

Query: 181 SLGLPAQRFRDYFKEKTSIVRLNHYPPCSLPEMALGVGRHNDPGVLTMLAQDDVGGLEVK 240
           SLGLP +RF DYFKE+ S  R+N YPPC  P++ALGVG H D  V+++LAQDDVGGL+V 
Sbjct: 181 SLGLPKERFHDYFKEQMSFFRINRYPPCPRPDLALGVGHHKDADVISLLAQDDVGGLQVS 240

Query: 241 RKRDGEWIRVKPVPDSYVVNVGNVTQV 266
           R+ DG W  ++PVP++ V+N+GN  +V
Sbjct: 241 RRSDGVWFPIRPVPNALVINIGNCMEV 264

The following BLAST results are available for this feature:

BLAST of Csor.00g277020 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FFF6	5.0e-52	38.29	Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1	[more]
Q39110	3.9e-49	35.31	Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2	[more]
O80449	2.6e-48	35.74	Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1	[more]
P0C5H5	2.2e-47	36.86	Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. indica OX=39946 GN=20ox2 PE=1 SV...	[more]
Q0JH50	2.2e-47	36.86	Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GA20OX2 PE=...	[more]

BLAST of Csor.00g277020 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6571999.1	3.65e-257	100.00	putative 2-oxoglutarate-dependent dioxygenase, partial [Cucurbita argyrosperma s...	[more]
XP_022952113.1	5.57e-252	97.66	probable 2-oxoglutarate-dependent dioxygenase At5g05600 [Cucurbita moschata]	[more]
KAG6571998.1	5.12e-248	97.08	putative 2-oxoglutarate-dependent dioxygenase, partial [Cucurbita argyrosperma s...	[more]
XP_022953057.1	2.08e-247	95.63	probable 2-oxoglutarate-dependent dioxygenase At5g05600 [Cucurbita moschata] >XP...	[more]
XP_023554291.1	3.49e-247	97.04	probable 2-oxoglutarate-dependent dioxygenase At5g05600 [Cucurbita pepo subsp. p...	[more]

BLAST of Csor.00g277020 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GJK3	2.69e-252	97.66	probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Cucurbita moschata OX...	[more]
A0A6J1GMB5	1.01e-247	95.63	probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Cucurbita moschata OX...	[more]
A0A6J1I5S1	1.32e-243	94.15	probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Cucurbita maxima OX=3...	[more]
A0A6J1I4K7	5.37e-243	93.27	probable 2-oxoglutarate-dependent dioxygenase At5g05600 isoform X3 OS=Cucurbita ...	[more]
A0A6J1I5R5	2.85e-226	90.85	probable 2-oxoglutarate-dependent dioxygenase At5g05600 isoform X2 OS=Cucurbita ...	[more]

BLAST of Csor.00g277020 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19000.1	2.0e-104	54.76	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19010.1	1.7e-95	48.39	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19000.2	5.8e-80	53.85	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19010.3	6.4e-79	42.82	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19010.2	7.1e-70	47.57	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 50..67 score: 53.54 coord: 283..301 score: 36.36 coord: 218..239 score: 37.6
None	No IPR available	PANTHER	PTHR10209:SF664	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE, NON-HEME DIOXYGENASE DOMAIN-CONTAINING PROTEIN-RELATED	coord: 2..326
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 2..326
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 4..330
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 196..295 e-value: 1.7E-29 score: 102.2
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 26..149 e-value: 1.9E-26 score: 93.2
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..335 e-value: 7.0E-104 score: 349.7
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 194..295 score: 14.583344

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g277020.m01	Csor.00g277020.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding