Csor.00g274510 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g274510
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionProtein kinase domain-containing protein
LocationCsor_Chr15: 10566828 .. 10569140 (-)
RNA-Seq ExpressionCsor.00g274510
SyntenyCsor.00g274510
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintronterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCAAATTCATTCTTCTCGTAGCTTCGGCCGCCATTTTTGTTCTTCTTCTAATCTCCATCTTCTTCTTCTTCTTCTTCCGCCATAAGCTCTCACTAACAGAGCATCGACACGACGTCGAGAGGCCCTGCAACGACGAACACAACAAAAAGGAACACACGCCGCCGCCGTCGCCGCCGTCGCTGGCAACTTTTGAGGGTGGCGAGGATTTGACGATCGATGAGATTCTAGAAGCTCCGGGGGAAGTGATTGGCAAATCCCACTATGGAACTCTGTACAAAGCTTCGTTACAGAGAACCCAATCGACCCGGTTACTTAGATTCCTCCGGCCGGGTGCCGGTGGAGGAGAGTACGACGTCGTTCGGTTTCTGGGTTCGATTAGGCATAGGAATTTAGTGCCGATGCTGGGATTCTACGCCGGCGGGAGGGGTGAGAGGCTTCTGGTTCACCCGTTTTATGGGCAGGGGAATTTGGCGGAGTTGATTAGAGGTATGTTTGAAATTTTTGGTGTGAGATTTTCTGTTTGTTTCTCGAGAAAGTGGAAGGGAAAATGGAGTTTAATTTGGAGTGGGATGTCGGCAACCAGATTTGTTGTCGGTTCTTGGTGGCTGTGGGCGTTTTGGTCCATAGGGAAATCAGAGATGGTGACCATCGTCCAGCGGTGCCTTTTTATTTGTCTCTTCTCCCCCGGGTTTTAAATGTCTAGGAGAGGTTTCCACACCCTTATAAAAATAGTTTGATTCCCGCTCTCTAACGTGAGATCTCACCATCCACTCACTTGGGGCTAAACATTTGGACACATAATTCGATGTCTGAGTATGATTTTATTTGTAACAGTAAAGTCTGCTGCTAGTAGATATTGTTCGGCTTATAGAAATGTTTAGTTCCCTCTCCAATGTAGGATTTCAGAATCTACTCCCTTGGAGCCAAACGTCATGTTACATCGCCCAGTGTCTAGCTGTGATAGTTCCCTCTCCAATGTGGGATTTCAGAATCTATCCCCTTGAGGCCAAACGTCATGGCACACCACCCAGTGTCTAGCTGTGATACTTCCCTCTCCAATGTGGGATTTCATAATTTACCCCCTTGAGGCCAAACGTCATGGCACACCACCCAGTGTCTAGCTGTGATACTTCCCTCTCCAATGTGGGATTTCAGAATCTACCCCCTTAAGGCCAAACGTCATGGCACACCGTCCAGTGTCTAGCTGTGATAGTTCCCTCTCCAATGTGAGATTTCAGAAACTATCCCCTTGAGGCCAAACGTCATGGCACACCACCCAGTGTCTAGCTGTGATAGTTCCCTCTCCAATGTGGGATTTCAGAATCTACCCCCTTGAGGCCAAACGTCATGGCACACCGCCCAGTGTCTAGCTGTGATACCATTTGTAACAGCTCAAGCGTACCGCGGGTAGATATTGTTTTCTTTGGACTTAACCTATCTCACAGTACACCTTTAGGGGCACAACATCTTGGCAGACCATCCCATGTCTAGATATGATACCATAAGTGACCGCCAAAGTCCACAACTAACAGATATTATTTTTGAATTTAAAACTGAAAGTTTATCTACCTTTTAAGAAATGTTTTGTTTTTTTGTTAAAATAATATATTTTAATCCATTTTTTTAGGTTTAATTTACTATATATTTTACATGAACTATATATATATTTTTTTATATATAAATAAAGATTTTTCATGTTGTTTTGCAAATTTATGTATTTTCTCTTCAAGAAATAAAATATTCTACAAATTACCTAAAGTTTTTATTTTCCTAATAATTTCAATTGTTCCACTTGTGATTGAACAGAACGAGAATCAGAGTCAAAAAATTGGGAGGTCATTAACAAAATCTCCCTCGGAATCGCAAGAGCCCTAGATCATCTCCACAACGGATTACAGAAACCAATACCTCATGGAAATCTCAAATCCAAGAACATTCTTCTCGATTCAAACTTTGAGCCGCACGTCTCCGATTTCGGCCTCCATCTCCTCCTCAACGCCGCCGCAACTCGAGAAATGCTCAAAGTTTCCGCCGCCTCCGGCTACAAGGCGCCGGAACTTATTAATATGACCGACGCCGATGAACGGACAGACGTCTTCAGCTACGGCAAGATCTTACTCGAGTTGCTTTCTGCCGAAGAGACGGGCGGAGACGGCGGCGATTGCGGTGGTTCTGAAGAGCGGACTCTCCGATGCCTTCTTCAGATCGGTGAGGCCTGTTGTTCTTCTTCGCCGGATCGCCGACCGGATTTCAAGGAAGTGATCGCGAAGATTGCAGAAATCGGAAGGATTGAGATGGATTTCAGATGA

mRNA sequence

ATGCTCAAATTCATTCTTCTCGTAGCTTCGGCCGCCATTTTTGTTCTTCTTCTAATCTCCATCTTCTTCTTCTTCTTCTTCCGCCATAAGCTCTCACTAACAGAGCATCGACACGACGTCGAGAGGCCCTGCAACGACGAACACAACAAAAAGGAACACACGCCGCCGCCGTCGCCGCCGTCGCTGGCAACTTTTGAGGGTGGCGAGGATTTGACGATCGATGAGATTCTAGAAGCTCCGGGGGAAGTGATTGGCAAATCCCACTATGGAACTCTGTACAAAGCTTCGTTACAGAGAACCCAATCGACCCGGTTACTTAGATTCCTCCGGCCGGGTGCCGGTGGAGGAGAGTACGACGTCGTTCGGTTTCTGGGTTCGATTAGGCATAGGAATTTAGTGCCGATGCTGGGATTCTACGCCGGCGGGAGGGGTGAGAGGCTTCTGGTTCACCCGTTTTATGGGCAGGGGAATTTGGCGGAGTTGATTAGAGAACGAGAATCAGAGTCAAAAAATTGGGAGGTCATTAACAAAATCTCCCTCGGAATCGCAAGAGCCCTAGATCATCTCCACAACGGATTACAGAAACCAATACCTCATGGAAATCTCAAATCCAAGAACATTCTTCTCGATTCAAACTTTGAGCCGCACGTCTCCGATTTCGGCCTCCATCTCCTCCTCAACGCCGCCGCAACTCGAGAAATGCTCAAAGTTTCCGCCGCCTCCGGCTACAAGGCGCCGGAACTTATTAATATGACCGACGCCGATGAACGGACAGACGTCTTCAGCTACGGCAAGATCTTACTCGAGTTGCTTTCTGCCGAAGAGACGGGCGGAGACGGCGGCGATTGCGGTGGTTCTGAAGAGCGGACTCTCCGATGCCTTCTTCAGATCGGTGAGGCCTGTTGTTCTTCTTCGCCGGATCGCCGACCGGATTTCAAGGAAGTGATCGCGAAGATTGCAGAAATCGGAAGGATTGAGATGGATTTCAGATGA

Coding sequence (CDS)

ATGCTCAAATTCATTCTTCTCGTAGCTTCGGCCGCCATTTTTGTTCTTCTTCTAATCTCCATCTTCTTCTTCTTCTTCTTCCGCCATAAGCTCTCACTAACAGAGCATCGACACGACGTCGAGAGGCCCTGCAACGACGAACACAACAAAAAGGAACACACGCCGCCGCCGTCGCCGCCGTCGCTGGCAACTTTTGAGGGTGGCGAGGATTTGACGATCGATGAGATTCTAGAAGCTCCGGGGGAAGTGATTGGCAAATCCCACTATGGAACTCTGTACAAAGCTTCGTTACAGAGAACCCAATCGACCCGGTTACTTAGATTCCTCCGGCCGGGTGCCGGTGGAGGAGAGTACGACGTCGTTCGGTTTCTGGGTTCGATTAGGCATAGGAATTTAGTGCCGATGCTGGGATTCTACGCCGGCGGGAGGGGTGAGAGGCTTCTGGTTCACCCGTTTTATGGGCAGGGGAATTTGGCGGAGTTGATTAGAGAACGAGAATCAGAGTCAAAAAATTGGGAGGTCATTAACAAAATCTCCCTCGGAATCGCAAGAGCCCTAGATCATCTCCACAACGGATTACAGAAACCAATACCTCATGGAAATCTCAAATCCAAGAACATTCTTCTCGATTCAAACTTTGAGCCGCACGTCTCCGATTTCGGCCTCCATCTCCTCCTCAACGCCGCCGCAACTCGAGAAATGCTCAAAGTTTCCGCCGCCTCCGGCTACAAGGCGCCGGAACTTATTAATATGACCGACGCCGATGAACGGACAGACGTCTTCAGCTACGGCAAGATCTTACTCGAGTTGCTTTCTGCCGAAGAGACGGGCGGAGACGGCGGCGATTGCGGTGGTTCTGAAGAGCGGACTCTCCGATGCCTTCTTCAGATCGGTGAGGCCTGTTGTTCTTCTTCGCCGGATCGCCGACCGGATTTCAAGGAAGTGATCGCGAAGATTGCAGAAATCGGAAGGATTGAGATGGATTTCAGATGA

Protein sequence

MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPPSLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRPGAGGGEYDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISLGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSAASGYKAPELINMTDADERTDVFSYGKILLELLSAEETGGDGGDCGGSEERTLRCLLQIGEACCSSSPDRRPDFKEVIAKIAEIGRIEMDFR
Homology
BLAST of Csor.00g274510 vs. ExPASy Swiss-Prot
Match: P33543 (Putative kinase-like protein TMKL1 OS=Arabidopsis thaliana OX=3702 GN=TMKL1 PE=1 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 4.5e-42
Identity = 107/280 (38.21%), Postives = 161/280 (57.50%), Query Frame = 0

Query: 7   LVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVER-PCNDEHNKKEHTPPPSPPSLATF 66
           L++ A +   LLI    +   + + S  E   D+E     DE  +KE         L  F
Sbjct: 308 LMSGAVVVASLLIG---YLQNKKRKSSIESEDDLEEGDEEDEIGEKE----GGEGKLVVF 367

Query: 67  EGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRPGA---GGGEYDVVR 126
           +GGE+LT+D++L A G+V+ K+ YGT+YKA L        LR LR G          V+R
Sbjct: 368 QGGENLTLDDVLNATGQVMEKTSYGTVYKAKLS-DGGNIALRLLREGTCKDRSSCLPVIR 427

Query: 127 FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERE--SESKNWEVINKISL 186
            LG IRH NLVP+  FY G RGE+LL++ +    +L +L+ E +    + NW   +KI+L
Sbjct: 428 QLGRIRHENLVPLRAFYQGKRGEKLLIYDYLPNISLHDLLHESKPRKPALNWARRHKIAL 487

Query: 187 GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSAA 246
           GIAR L +LH G + PI HGN++SKN+L+D  F   +++FGL  ++  A   E++  + +
Sbjct: 488 GIARGLAYLHTGQEVPIIHGNIRSKNVLVDDFFFARLTEFGLDKIMVQAVADEIVSQAKS 547

Query: 247 SGYKAPELINMTDADERTDVFSYGKILLELLSAEETGGDG 281
            GYKAPEL  M   + R+DV+++G +LLE+L  ++ G  G
Sbjct: 548 DGYKAPELHKMKKCNPRSDVYAFGILLLEILMGKKPGKSG 579

BLAST of Csor.00g274510 vs. ExPASy Swiss-Prot
Match: Q9SCT4 (Probably inactive leucine-rich repeat receptor-like protein kinase IMK2 OS=Arabidopsis thaliana OX=3702 GN=IMK2 PE=1 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 8.2e-36
Identity = 99/290 (34.14%), Postives = 151/290 (52.07%), Query Frame = 0

Query: 62  LATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFL-RPGAGGGEYD- 121
           L  F+G    T D++L A  E++GKS YGT YKA+L+      + R   +   G  E++ 
Sbjct: 521 LVHFDGPFVFTADDLLCATAEIMGKSTYGTAYKATLEDGNEVAVKRLREKTTKGVKEFEG 580

Query: 122 VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESK-NWEVINKI 181
            V  LG IRH+NL+ +  +Y G +GE+LLV  +  +G+L+  +  R  E+   WE   KI
Sbjct: 581 EVTALGKIRHQNLLALRAYYLGPKGEKLLVFDYMSKGSLSAFLHARGPETLIPWETRMKI 640

Query: 182 SLGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVS 241
           + GI+R L HLH+   + + H NL + NILLD     H++D+GL  L+ AAA   ++  +
Sbjct: 641 AKGISRGLAHLHS--NENMIHENLTASNILLDEQTNAHIADYGLSRLMTAAAATNVIATA 700

Query: 242 AASGYKAPELINMTDADERTDVFSYGKILLELLSAEETG--GDGGDCGG------SEERT 301
              GY+APE   + +A  +TDV+S G I+LELL+ +  G   +G D          EE T
Sbjct: 701 GTLGYRAPEFSKIKNASAKTDVYSLGIIILELLTGKSPGEPTNGMDLPQWVASIVKEEWT 760

Query: 302 ------------------LRCLLQIGEACCSSSPDRRPDFKEVIAKIAEI 323
                             L   L++   C   SP  RP+  +V+ ++ EI
Sbjct: 761 NEVFDLELMRETQSVGDELLNTLKLALHCVDPSPAARPEANQVVEQLEEI 808

BLAST of Csor.00g274510 vs. ExPASy Swiss-Prot
Match: Q9LVI6 (Probable inactive receptor kinase RLK902 OS=Arabidopsis thaliana OX=3702 GN=RLK902 PE=1 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 4.5e-34
Identity = 94/286 (32.87%), Postives = 149/286 (52.10%), Query Frame = 0

Query: 73  IDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRPGAGGGEY-DVVRFLGSIRHRN 132
           ++++L A  EV+GK  +GT YKA L       + R         E+ + +  +G++ H N
Sbjct: 360 LEDLLRASAEVLGKGTFGTAYKAVLDAVTLVAVKRLKDVTMADREFKEKIEVVGAMDHEN 419

Query: 133 LVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESK---NWEVINKISLGIARALDH 192
           LVP+  +Y  G  E+LLV+ F   G+L+ L+   +   +   NWEV + I+LG AR LD+
Sbjct: 420 LVPLRAYYYSG-DEKLLVYDFMPMGSLSALLHGNKGAGRPPLNWEVRSGIALGAARGLDY 479

Query: 193 LHNGLQKPI-PHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSAASGYKAPE 252
           LH+  Q P+  HGN+KS NILL ++ +  VSDFGL  L++A++T      + A+GY+APE
Sbjct: 480 LHS--QDPLSSHGNVKSSNILLTNSHDARVSDFGLAQLVSASSTTP----NRATGYRAPE 539

Query: 253 LINMTDADERTDVFSYGKILLELLSAEETGG-----DGGDCGG----------------- 312
           + +     ++ DV+S+G +LLELL+ +         +G D                    
Sbjct: 540 VTDPRRVSQKADVYSFGVVLLELLTGKAPSNSVMNEEGMDLARWVHSVAREEWRNEVFDS 599

Query: 313 ---------SEERTLRCLLQIGEACCSSSPDRRPDFKEVIAKIAEI 323
                    S E  +  +LQ+G  C    PD+RP   EV+ +I E+
Sbjct: 600 ELMSIETVVSVEEEMAEMLQLGIDCTEQHPDKRPVMVEVVRRIQEL 638

BLAST of Csor.00g274510 vs. ExPASy Swiss-Prot
Match: Q9LP77 (Probable inactive receptor kinase At1g48480 OS=Arabidopsis thaliana OX=3702 GN=RKL1 PE=1 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 2.9e-33
Identity = 88/283 (31.10%), Postives = 145/283 (51.24%), Query Frame = 0

Query: 73  IDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRPGAGGGEY-DVVRFLGSIRHRN 132
           ++++L A  EV+GK  +GT YKA L       + R         E+ + +  +G++ H N
Sbjct: 366 LEDLLRASAEVLGKGTFGTAYKAVLDAVTVVAVKRLKDVMMADKEFKEKIELVGAMDHEN 425

Query: 133 LVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESK---NWEVINKISLGIARALDH 192
           LVP+  +Y   R E+LLV+ F   G+L+ L+       +   NW+V ++I++G AR LD+
Sbjct: 426 LVPLRAYYF-SRDEKLLVYDFMPMGSLSALLHGNRGAGRSPLNWDVRSRIAIGAARGLDY 485

Query: 193 LHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSAASGYKAPEL 252
           LH+       HGN+KS NILL  + +  VSDFGL  L+ ++AT      + A+GY+APE+
Sbjct: 486 LHS-QGTSTSHGNIKSSNILLTKSHDAKVSDFGLAQLVGSSATNP----NRATGYRAPEV 545

Query: 253 INMTDADERTDVFSYGKILLELLSAEETGG-----DGGD--------------------- 312
            +     ++ DV+S+G +LLEL++ +         +G D                     
Sbjct: 546 TDPKRVSQKGDVYSFGVVLLELITGKAPSNSVMNEEGVDLPRWVKSVARDEWRREVFDSE 605

Query: 313 ---CGGSEERTLRCLLQIGEACCSSSPDRRPDFKEVIAKIAEI 323
                  EE  +  ++Q+G  C S  PD+RP+  EV+ K+  +
Sbjct: 606 LLSLATDEEEMMAEMVQLGLECTSQHPDQRPEMSEVVRKMENL 642

BLAST of Csor.00g274510 vs. ExPASy Swiss-Prot
Match: C0LGP9 (Probable leucine-rich repeat receptor-like protein kinase IMK3 OS=Arabidopsis thaliana OX=3702 GN=IMK3 PE=1 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 3.8e-33
Identity = 109/359 (30.36%), Postives = 171/359 (47.63%), Query Frame = 0

Query: 3   KFILLVASAAIFVLLLISI-FFFFFFRHKLSLTEHRHDVERP----CNDEHNKKEHTPPP 62
           K I+L+AS A+ +++LI +       R K + T+ +     P       E   +      
Sbjct: 408 KDIILIASGALLIVMLILVCVLCCLLRKKANETKAKGGEAGPGAVAAKTEKGGEAEAGGE 467

Query: 63  SPPSLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRPGAGGGE 122
           +   L  F+G    T D++L A  E++GKS YGT+YKA+L+      + R LR      +
Sbjct: 468 TGGKLVHFDGPMAFTADDLLCATAEIMGKSTYGTVYKATLEDGSQVAVKR-LREKITKSQ 527

Query: 123 YDV---VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESK-NWE 182
            +    +  LG IRH NL+ +  +Y G +GE+L+V  +  +G+LA  +  R  +   NW 
Sbjct: 528 KEFENEINVLGRIRHPNLLALRAYYLGPKGEKLVVFDYMSRGSLATFLHARGPDVHINWP 587

Query: 183 VINKISLGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATRE 242
               +  G+AR L +LH      I HGNL S N+LLD N    +SD+GL  L+ AAA   
Sbjct: 588 TRMSLIKGMARGLFYLHT--HANIIHGNLTSSNVLLDENITAKISDYGLSRLMTAAAGSS 647

Query: 243 MLKVSAASGYKAPELINMTDADERTDVFSYGKILLELLSAE------------------- 302
           ++  + A GY+APEL  +  A+ +TDV+S G I+LELL+ +                   
Sbjct: 648 VIATAGALGYRAPELSKLKKANTKTDVYSLGVIILELLTGKSPSEALNGVDLPQWVATAV 707

Query: 303 -----------ETGGDGGDCGGSEERTLRCLLQIGEACCSSSPDRRPDFKEVIAKIAEI 323
                      E   D    G     TL+  L     C  ++P  RP+ ++V+ ++ EI
Sbjct: 708 KEEWTNEVFDLELLNDVNTMGDEILNTLKLALH----CVDATPSTRPEAQQVMTQLGEI 759

BLAST of Csor.00g274510 vs. NCBI nr
Match: KAG6579628.1 (putative kinase-like protein TMKL1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 654 bits (1687), Expect = 1.02e-236
Identity = 330/330 (100.00%), Postives = 330/330 (100.00%), Query Frame = 0

Query: 1   MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPP 60
           MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPP
Sbjct: 1   MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPP 60

Query: 61  SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRPGAGGGEYDV 120
           SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRPGAGGGEYDV
Sbjct: 61  SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRPGAGGGEYDV 120

Query: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISL 180
           VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISL
Sbjct: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISL 180

Query: 181 GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSAA 240
           GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSAA
Sbjct: 181 GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSAA 240

Query: 241 SGYKAPELINMTDADERTDVFSYGKILLELLSAEETGGDGGDCGGSEERTLRCLLQIGEA 300
           SGYKAPELINMTDADERTDVFSYGKILLELLSAEETGGDGGDCGGSEERTLRCLLQIGEA
Sbjct: 241 SGYKAPELINMTDADERTDVFSYGKILLELLSAEETGGDGGDCGGSEERTLRCLLQIGEA 300

Query: 301 CCSSSPDRRPDFKEVIAKIAEIGRIEMDFR 330
           CCSSSPDRRPDFKEVIAKIAEIGRIEMDFR
Sbjct: 301 CCSSSPDRRPDFKEVIAKIAEIGRIEMDFR 330

BLAST of Csor.00g274510 vs. NCBI nr
Match: KAG7017083.1 (putative kinase-like protein TMKL1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 647 bits (1670), Expect = 4.12e-234
Identity = 329/331 (99.40%), Postives = 329/331 (99.40%), Query Frame = 0

Query: 1   MLKFILLVASAAIFVLLLISIFFFFFF-RHKLSLTEHRHDVERPCNDEHNKKEHTPPPSP 60
           MLKFILLVASAAIFVLLLISIFFFFFF RHKLSLTEHRHDVERPCNDEHNKKEHTPPPSP
Sbjct: 1   MLKFILLVASAAIFVLLLISIFFFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSP 60

Query: 61  PSLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRPGAGGGEYD 120
           PSL TFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRPGAGGGEYD
Sbjct: 61  PSLETFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRPGAGGGEYD 120

Query: 121 VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKIS 180
           VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKIS
Sbjct: 121 VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKIS 180

Query: 181 LGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSA 240
           LGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSA
Sbjct: 181 LGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSA 240

Query: 241 ASGYKAPELINMTDADERTDVFSYGKILLELLSAEETGGDGGDCGGSEERTLRCLLQIGE 300
           ASGYKAPELINMTDADERTDVFSYGKILLELLSAEETGGDGGDCGGSEERTLRCLLQIGE
Sbjct: 241 ASGYKAPELINMTDADERTDVFSYGKILLELLSAEETGGDGGDCGGSEERTLRCLLQIGE 300

Query: 301 ACCSSSPDRRPDFKEVIAKIAEIGRIEMDFR 330
           ACCSSSPDRRPDFKEVIAKIAEIGRIEMDFR
Sbjct: 301 ACCSSSPDRRPDFKEVIAKIAEIGRIEMDFR 331

BLAST of Csor.00g274510 vs. NCBI nr
Match: XP_022929086.1 (putative kinase-like protein TMKL1 [Cucurbita moschata] >XP_022929087.1 putative kinase-like protein TMKL1 [Cucurbita moschata] >XP_022929088.1 putative kinase-like protein TMKL1 [Cucurbita moschata])

HSP 1 Score: 643 bits (1658), Expect = 2.58e-232
Identity = 326/330 (98.79%), Postives = 328/330 (99.39%), Query Frame = 0

Query: 1   MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPP 60
           MLKF+LLVASAAIFVLLLISIFFFFF RHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPP
Sbjct: 1   MLKFMLLVASAAIFVLLLISIFFFFF-RHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPP 60

Query: 61  SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRPGAGGGEYDV 120
           SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQ TQ+TRLLRFLRPGAGGGEYDV
Sbjct: 61  SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQATRLLRFLRPGAGGGEYDV 120

Query: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISL 180
           VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISL
Sbjct: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISL 180

Query: 181 GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSAA 240
           GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSAA
Sbjct: 181 GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSAA 240

Query: 241 SGYKAPELINMTDADERTDVFSYGKILLELLSAEETGGDGGDCGGSEERTLRCLLQIGEA 300
           SGYKAPELINMTDADERTDVFSYGKILLELLSAEETGGDGGDCGGSEERTLRCLLQIGEA
Sbjct: 241 SGYKAPELINMTDADERTDVFSYGKILLELLSAEETGGDGGDCGGSEERTLRCLLQIGEA 300

Query: 301 CCSSSPDRRPDFKEVIAKIAEIGRIEMDFR 330
           CCSSSPDRRPDFKEVIAKIAEIGRIEMDFR
Sbjct: 301 CCSSSPDRRPDFKEVIAKIAEIGRIEMDFR 329

BLAST of Csor.00g274510 vs. NCBI nr
Match: XP_023520536.1 (putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo] >XP_023520537.1 putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo] >XP_023550283.1 putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo] >XP_023550284.1 putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 633 bits (1632), Expect = 1.36e-227
Identity = 322/330 (97.58%), Postives = 325/330 (98.48%), Query Frame = 0

Query: 1   MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPP 60
           MLKFILLVASAAIFVLLLISIFFFFF RHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPP
Sbjct: 48  MLKFILLVASAAIFVLLLISIFFFFF-RHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPP 107

Query: 61  SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRPGAGGGEYDV 120
           SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQ TQSTRLLRFLRPGAGGGEYDV
Sbjct: 108 SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEYDV 167

Query: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISL 180
           VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISL
Sbjct: 168 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISL 227

Query: 181 GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSAA 240
           GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREML+VSAA
Sbjct: 228 GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSAA 287

Query: 241 SGYKAPELINMTDADERTDVFSYGKILLELLSAEETGGDGGDCGGSEERTLRCLLQIGEA 300
           SGYKAPELINM+DADERTDVFSYGKILLELLSA+ETGGDGGDCG SEERTLRCLLQIGEA
Sbjct: 288 SGYKAPELINMSDADERTDVFSYGKILLELLSAKETGGDGGDCGVSEERTLRCLLQIGEA 347

Query: 301 CCSSSPDRRPDFKEVIAKIAEIGRIEMDFR 330
           CCSSSPD RPDFKEVIAKI EIGRIEMDFR
Sbjct: 348 CCSSSPDLRPDFKEVIAKITEIGRIEMDFR 376

BLAST of Csor.00g274510 vs. NCBI nr
Match: XP_022969804.1 (putative kinase-like protein TMKL1 [Cucurbita maxima])

HSP 1 Score: 613 bits (1581), Expect = 1.96e-220
Identity = 310/328 (94.51%), Postives = 319/328 (97.26%), Query Frame = 0

Query: 1   MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPP 60
           MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVERPC D++ KKEHTPPPSPP
Sbjct: 1   MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVERPCTDQNKKKEHTPPPSPP 60

Query: 61  SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRPGAGGGEYDV 120
           SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQ TQSTRLLRFLRPGAGGGEYDV
Sbjct: 61  SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEYDV 120

Query: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISL 180
           VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLA+LIR  ESE+KNWEVINKISL
Sbjct: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLADLIRVGESETKNWEVINKISL 180

Query: 181 GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSAA 240
           GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREML+VSAA
Sbjct: 181 GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSAA 240

Query: 241 SGYKAPELINMTDADERTDVFSYGKILLELLSAEETGGDGGDCGGSEERTLRCLLQIGEA 300
           SGYKAPELINM DADERTD+FSYGKILLELLSA+ETGGDGGDCG SEERTLRC+LQIGEA
Sbjct: 241 SGYKAPELINMNDADERTDIFSYGKILLELLSAKETGGDGGDCGVSEERTLRCILQIGEA 300

Query: 301 CCSSSPDRRPDFKEVIAKIAEIGRIEMD 328
           C SSSPD RP+FKEVIAKIAEIGRIEMD
Sbjct: 301 CSSSSPDLRPNFKEVIAKIAEIGRIEMD 328

BLAST of Csor.00g274510 vs. ExPASy TrEMBL
Match: A0A6J1ELS2 (putative kinase-like protein TMKL1 OS=Cucurbita moschata OX=3662 GN=LOC111435777 PE=4 SV=1)

HSP 1 Score: 643 bits (1658), Expect = 1.25e-232
Identity = 326/330 (98.79%), Postives = 328/330 (99.39%), Query Frame = 0

Query: 1   MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPP 60
           MLKF+LLVASAAIFVLLLISIFFFFF RHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPP
Sbjct: 1   MLKFMLLVASAAIFVLLLISIFFFFF-RHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPP 60

Query: 61  SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRPGAGGGEYDV 120
           SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQ TQ+TRLLRFLRPGAGGGEYDV
Sbjct: 61  SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQATRLLRFLRPGAGGGEYDV 120

Query: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISL 180
           VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISL
Sbjct: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISL 180

Query: 181 GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSAA 240
           GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSAA
Sbjct: 181 GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSAA 240

Query: 241 SGYKAPELINMTDADERTDVFSYGKILLELLSAEETGGDGGDCGGSEERTLRCLLQIGEA 300
           SGYKAPELINMTDADERTDVFSYGKILLELLSAEETGGDGGDCGGSEERTLRCLLQIGEA
Sbjct: 241 SGYKAPELINMTDADERTDVFSYGKILLELLSAEETGGDGGDCGGSEERTLRCLLQIGEA 300

Query: 301 CCSSSPDRRPDFKEVIAKIAEIGRIEMDFR 330
           CCSSSPDRRPDFKEVIAKIAEIGRIEMDFR
Sbjct: 301 CCSSSPDRRPDFKEVIAKIAEIGRIEMDFR 329

BLAST of Csor.00g274510 vs. ExPASy TrEMBL
Match: A0A6J1I3Q5 (putative kinase-like protein TMKL1 OS=Cucurbita maxima OX=3661 GN=LOC111468894 PE=4 SV=1)

HSP 1 Score: 613 bits (1581), Expect = 9.51e-221
Identity = 310/328 (94.51%), Postives = 319/328 (97.26%), Query Frame = 0

Query: 1   MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPP 60
           MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVERPC D++ KKEHTPPPSPP
Sbjct: 1   MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVERPCTDQNKKKEHTPPPSPP 60

Query: 61  SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRPGAGGGEYDV 120
           SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQ TQSTRLLRFLRPGAGGGEYDV
Sbjct: 61  SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEYDV 120

Query: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISL 180
           VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLA+LIR  ESE+KNWEVINKISL
Sbjct: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLADLIRVGESETKNWEVINKISL 180

Query: 181 GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSAA 240
           GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREML+VSAA
Sbjct: 181 GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSAA 240

Query: 241 SGYKAPELINMTDADERTDVFSYGKILLELLSAEETGGDGGDCGGSEERTLRCLLQIGEA 300
           SGYKAPELINM DADERTD+FSYGKILLELLSA+ETGGDGGDCG SEERTLRC+LQIGEA
Sbjct: 241 SGYKAPELINMNDADERTDIFSYGKILLELLSAKETGGDGGDCGVSEERTLRCILQIGEA 300

Query: 301 CCSSSPDRRPDFKEVIAKIAEIGRIEMD 328
           C SSSPD RP+FKEVIAKIAEIGRIEMD
Sbjct: 301 CSSSSPDLRPNFKEVIAKIAEIGRIEMD 328

BLAST of Csor.00g274510 vs. ExPASy TrEMBL
Match: A0A6J1H0H8 (putative kinase-like protein TMKL1 OS=Cucurbita moschata OX=3662 GN=LOC111459294 PE=4 SV=1)

HSP 1 Score: 480 bits (1235), Expect = 4.05e-168
Identity = 253/337 (75.07%), Postives = 277/337 (82.20%), Query Frame = 0

Query: 1   MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPP 60
           MLK IL+VASA IFVLLL+SIFF    RHKLS T HR+D+E P  D + KK+ T   +  
Sbjct: 1   MLKLILIVASATIFVLLLVSIFFL---RHKLSKTNHRNDIENPSPDNNQKKQDTAAATVE 60

Query: 61  SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRPGAGGGEYD- 120
            L TFEGGEDLTIDEILEAPGEVIGK HYGTLYKASLQ TQ T LL FLRP A     D 
Sbjct: 61  ELTTFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDD 120

Query: 121 VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKIS 180
           VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYG+GNL+E I   ESESKNWE+I KIS
Sbjct: 121 VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKIS 180

Query: 181 LGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSA 240
           LGIA+ALDHLH+GL KP+PHGNLK KNI LD +FEP+VSDFGLHLLLNAAATRE+L+ S+
Sbjct: 181 LGIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASS 240

Query: 241 ASGYKAPELINMTDADERTDVFSYGKILLELLSAEET--------GGDGGDCGGSEERTL 300
           ASGY APEL+ MTDADERTDV+SYGKILLELLSA++T        GGDGGDC  S+ER L
Sbjct: 241 ASGYNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNL 300

Query: 301 RCLLQIGEACCSSSPDRRPDFKEVIAKIAEIGRIEMD 328
           +CL QIGEACCSSSP RRPDFKEVI KI EIGRIEMD
Sbjct: 301 QCLFQIGEACCSSSPIRRPDFKEVIEKIEEIGRIEMD 334

BLAST of Csor.00g274510 vs. ExPASy TrEMBL
Match: A0A6J1K4E4 (putative kinase-like protein TMKL1 OS=Cucurbita maxima OX=3661 GN=LOC111490677 PE=4 SV=1)

HSP 1 Score: 479 bits (1232), Expect = 1.16e-167
Identity = 252/337 (74.78%), Postives = 275/337 (81.60%), Query Frame = 0

Query: 1   MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPP 60
           MLK IL+VASA IFVLLL+SIFFF   RHKLS + HRHD+E P  D + KK+ T   +  
Sbjct: 1   MLKLILIVASATIFVLLLLSIFFF---RHKLSKSNHRHDIENPSPDNNQKKQDTTAATVE 60

Query: 61  SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRPGAGGGEYD- 120
            L TF+GGEDLTIDEILEAPGEVIGK HYGTLYKASLQ TQ   LL FLRP A     D 
Sbjct: 61  ELTTFQGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRATLLMFLRPAAVESSVDD 120

Query: 121 VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKIS 180
           VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYG+GNL+E I   ESESKNWE+I KIS
Sbjct: 121 VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKIS 180

Query: 181 LGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSA 240
           LGIA+ALDHLH+GL KP+PHGNLK  NI LD NFEP+VSDFGLHLLLNAAATRE+L+ S+
Sbjct: 181 LGIAKALDHLHHGLPKPVPHGNLKPMNIFLDPNFEPYVSDFGLHLLLNAAATRELLQASS 240

Query: 241 ASGYKAPELINMTDADERTDVFSYGKILLELLSAEET--------GGDGGDCGGSEERTL 300
           ASGY APEL+ MTDADERTDV+SYGKILLELLSA+ET        GGDGGDC  S+ER L
Sbjct: 241 ASGYNAPELVKMTDADERTDVYSYGKILLELLSAKETMNQTAVACGGDGGDCSVSKERNL 300

Query: 301 RCLLQIGEACCSSSPDRRPDFKEVIAKIAEIGRIEMD 328
           +CL QIGEACC SSP RRPDFKEVI KI EIGRIEMD
Sbjct: 301 QCLFQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD 334

BLAST of Csor.00g274510 vs. ExPASy TrEMBL
Match: A0A5A7TJN4 (Putative kinase-like protein TMKL1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1167G00210 PE=4 SV=1)

HSP 1 Score: 437 bits (1123), Expect = 4.10e-151
Identity = 240/337 (71.22%), Postives = 272/337 (80.71%), Query Frame = 0

Query: 1   MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVERPCN----DEHNKKEHTPP 60
           MLK IL +ASA +F+LLLI+IF     R KLS     HD+E+       + +NK +HTP 
Sbjct: 1   MLKLILPLASATLFLLLLITIFLL---RLKLSKN---HDIEKSSTTHKTNNNNKTDHTPL 60

Query: 61  PSPPSLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRPGAGGG 120
           P PP L TFEGG+DLTIDEILEAPGEVIGKSHYGTLYKA LQ TQ+ RLLRFLRP A GG
Sbjct: 61  PPPPELTTFEGGQDLTIDEILEAPGEVIGKSHYGTLYKAVLQTTQTIRLLRFLRPAAAGG 120

Query: 121 E--YDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEV 180
           E  +D+VRFLGS+RHRNLVP+LGFYAGGRGE+LLVHPFY  GNLAE IR+ + ESKNWEV
Sbjct: 121 EDQHDLVRFLGSVRHRNLVPLLGFYAGGRGEKLLVHPFYEHGNLAEFIRD-DGESKNWEV 180

Query: 181 INKISLGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREM 240
           I KI++GIARAL HLH+GLQKPI HGNL SKN+LLD N +PHVSDFGLHLLLN+AATREM
Sbjct: 181 ICKIAIGIARALHHLHSGLQKPISHGNLNSKNVLLDQNLDPHVSDFGLHLLLNSAATREM 240

Query: 241 LKVSAASGYKAPELINMTDADERTDVFSYGKILLELL-SAEETGGDG----GDCGGSEER 300
           L+VSA+SGYKAPELINMT ADERTDV+SYGKILLELL SA+E         GD G S E+
Sbjct: 241 LQVSASSGYKAPELINMTHADERTDVYSYGKILLELLISAKEPTIRNPVATGDSGVSGEQ 300

Query: 301 TLRCLLQIGEACCSSSPDRRPDFKEVIAKIAEIGRIE 326
            LRCL+QIGE CC SSP+ RPDF+EVIAKI EI RIE
Sbjct: 301 ILRCLVQIGEDCCRSSPNLRPDFREVIAKIEEIRRIE 330

BLAST of Csor.00g274510 vs. TAIR 10
Match: AT5G07620.1 (Protein kinase superfamily protein )

HSP 1 Score: 304.7 bits (779), Expect = 9.2e-83
Identity = 179/363 (49.31%), Postives = 233/363 (64.19%), Query Frame = 0

Query: 1   MLKFILLVASAAIFVLLLISIFFFFFFRHK--LSLTEHRHDVERPCNDEHNKKEHTPPPS 60
           +L  +LL  S + F   LI IFF  F R K   S    ++DVE   + +HNK+  +    
Sbjct: 5   LLLTLLLGVSLSAF---LIVIFFIIFLRRKESSSTESDQYDVE---SLDHNKQGFS--SE 64

Query: 61  PPSLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRP----GAG 120
              L  F+GGEDLTI +IL+APGEVIGKS YGTLYKASLQR+   R+LRFLRP     + 
Sbjct: 65  TEELVIFQGGEDLTICDILDAPGEVIGKSSYGTLYKASLQRSGKIRVLRFLRPVCTVRSD 124

Query: 121 GGEYD-VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWE 180
             E++ ++  LG +RH NLVP+LGFYAG RGE+L+VHPF+G GNL++ IR  + ES+ W 
Sbjct: 125 SKEFNGIIETLGFVRHENLVPLLGFYAGNRGEKLMVHPFFGSGNLSDFIRSGDDESRKWI 184

Query: 181 VINKISLGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATRE 240
            I +I++GI++ALDHLH G+QKPI HGNLKSKN+LL S+FEP +SDFGLHLLLN +A +E
Sbjct: 185 NILRITIGISKALDHLHTGMQKPIVHGNLKSKNVLLSSSFEPRISDFGLHLLLNLSAGQE 244

Query: 241 MLKVSAASGYKAPELINMTDADERTDVFSYGKILLELLSAEE------TGGD-------- 300
           +L VSAA GYKAPELI M D  + +DV+S G I+LEL+S +E      TG D        
Sbjct: 245 ILDVSAAEGYKAPELIKMKDVSKESDVYSLGVIMLELVSGKEPINENATGDDEFYLPDFM 304

Query: 301 ------------------GGDCGGSEERTLRCLLQIGEACCSSSPDRRPDFKEVIAKIAE 325
                             G D   SEE  L+   Q+  +CCS SP  RP+ K+V+ K+ E
Sbjct: 305 RNAVLDHRLSDLYRPEILGSDDNLSEECVLK-YFQLAMSCCSPSPSLRPNVKQVLRKLEE 358

BLAST of Csor.00g274510 vs. TAIR 10
Match: AT5G61570.2 (Protein kinase superfamily protein )

HSP 1 Score: 287.0 bits (733), Expect = 2.0e-77
Identity = 166/355 (46.76%), Postives = 222/355 (62.54%), Query Frame = 0

Query: 1   MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLT-EHRHDVERPCNDEHNKKEHTPPPS- 60
           ++K IL V+    F++++  I  F F R   + T E ++DVE P    + K+E +   S 
Sbjct: 6   LVKLILAVSLPTTFLIMIAIIIIFIFCRRTTTETNEVQYDVESP----YEKQEFSDNGSE 65

Query: 61  -PPSLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRP----GA 120
               L  F GGEDLTI +IL+APGEVIGKS YGTLYKA+LQR+   R+LRFLRP     +
Sbjct: 66  TEEELIIFNGGEDLTICDILDAPGEVIGKSSYGTLYKATLQRSGKVRVLRFLRPLCAVNS 125

Query: 121 GGGEYD-VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYG-QGNLAELIRERESESKN 180
              E++ V+  LG +RH NLVP+LGFY G RGE+L++HPF+G  GNL+  I+  + ++  
Sbjct: 126 DSKEFNGVIESLGFVRHDNLVPLLGFYVGNRGEKLMIHPFFGSSGNLSAFIKCGDVDAHK 185

Query: 181 WEVINKISLGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAAT 240
           W  I  I++GIA+ALDHLH G+QKPI HGNLKSKN+LLD +F P VSDFGLHLLLN AA 
Sbjct: 186 WSNILSITIGIAKALDHLHTGMQKPIVHGNLKSKNVLLDKSFRPRVSDFGLHLLLNLAAG 245

Query: 241 REMLKVSAASGYKAPELINMTDADERTDVFSYGKILLELLSAEETGGDG----------- 300
           +E+L+ SAA GYKAPELI M +  + +DV+S+G I+LEL+S +E                
Sbjct: 246 QEVLEASAAEGYKAPELIKMKEVSKESDVYSFGVIMLELVSGKEPTNKNPTGSVLDRNRL 305

Query: 301 -------------GDCGGSEERTLRCLLQIGEACCSSSPDRRPDFKEVIAKIAEI 323
                         D  G  E  +    Q+  +CCS SP  RP FK+V+ K+ EI
Sbjct: 306 SDLYRPEIIRRCLKDGNGVTEECVLEYFQLAMSCCSPSPTLRPSFKQVLRKLEEI 356

BLAST of Csor.00g274510 vs. TAIR 10
Match: AT5G61570.1 (Protein kinase superfamily protein )

HSP 1 Score: 282.3 bits (721), Expect = 4.9e-76
Identity = 166/358 (46.37%), Postives = 222/358 (62.01%), Query Frame = 0

Query: 1   MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLT-EHRHDVERPCNDEHNKKEHTPPPS- 60
           ++K IL V+    F++++  I  F F R   + T E ++DVE P    + K+E +   S 
Sbjct: 6   LVKLILAVSLPTTFLIMIAIIIIFIFCRRTTTETNEVQYDVESP----YEKQEFSDNGSE 65

Query: 61  -PPSLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRP----GA 120
               L  F GGEDLTI +IL+APGEVIGKS YGTLYKA+LQR+   R+LRFLRP     +
Sbjct: 66  TEEELIIFNGGEDLTICDILDAPGEVIGKSSYGTLYKATLQRSGKVRVLRFLRPLCAVNS 125

Query: 121 GGGEYD-VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYG-QGNLAELIR---ERESE 180
              E++ V+  LG +RH NLVP+LGFY G RGE+L++HPF+G  GNL+  I+     + +
Sbjct: 126 DSKEFNGVIESLGFVRHDNLVPLLGFYVGNRGEKLMIHPFFGSSGNLSAFIKFLAGGDVD 185

Query: 181 SKNWEVINKISLGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNA 240
           +  W  I  I++GIA+ALDHLH G+QKPI HGNLKSKN+LLD +F P VSDFGLHLLLN 
Sbjct: 186 AHKWSNILSITIGIAKALDHLHTGMQKPIVHGNLKSKNVLLDKSFRPRVSDFGLHLLLNL 245

Query: 241 AATREMLKVSAASGYKAPELINMTDADERTDVFSYGKILLELLSAEETGGDG-------- 300
           AA +E+L+ SAA GYKAPELI M +  + +DV+S+G I+LEL+S +E             
Sbjct: 246 AAGQEVLEASAAEGYKAPELIKMKEVSKESDVYSFGVIMLELVSGKEPTNKNPTGSVLDR 305

Query: 301 ----------------GDCGGSEERTLRCLLQIGEACCSSSPDRRPDFKEVIAKIAEI 323
                            D  G  E  +    Q+  +CCS SP  RP FK+V+ K+ EI
Sbjct: 306 NRLSDLYRPEIIRRCLKDGNGVTEECVLEYFQLAMSCCSPSPTLRPSFKQVLRKLEEI 359

BLAST of Csor.00g274510 vs. TAIR 10
Match: AT3G24660.1 (transmembrane kinase-like 1 )

HSP 1 Score: 173.3 bits (438), Expect = 3.2e-43
Identity = 107/280 (38.21%), Postives = 161/280 (57.50%), Query Frame = 0

Query: 7   LVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVER-PCNDEHNKKEHTPPPSPPSLATF 66
           L++ A +   LLI    +   + + S  E   D+E     DE  +KE         L  F
Sbjct: 308 LMSGAVVVASLLIG---YLQNKKRKSSIESEDDLEEGDEEDEIGEKE----GGEGKLVVF 367

Query: 67  EGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFLRPGA---GGGEYDVVR 126
           +GGE+LT+D++L A G+V+ K+ YGT+YKA L        LR LR G          V+R
Sbjct: 368 QGGENLTLDDVLNATGQVMEKTSYGTVYKAKLS-DGGNIALRLLREGTCKDRSSCLPVIR 427

Query: 127 FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERE--SESKNWEVINKISL 186
            LG IRH NLVP+  FY G RGE+LL++ +    +L +L+ E +    + NW   +KI+L
Sbjct: 428 QLGRIRHENLVPLRAFYQGKRGEKLLIYDYLPNISLHDLLHESKPRKPALNWARRHKIAL 487

Query: 187 GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSAA 246
           GIAR L +LH G + PI HGN++SKN+L+D  F   +++FGL  ++  A   E++  + +
Sbjct: 488 GIARGLAYLHTGQEVPIIHGNIRSKNVLVDDFFFARLTEFGLDKIMVQAVADEIVSQAKS 547

Query: 247 SGYKAPELINMTDADERTDVFSYGKILLELLSAEETGGDG 281
            GYKAPEL  M   + R+DV+++G +LLE+L  ++ G  G
Sbjct: 548 DGYKAPELHKMKKCNPRSDVYAFGILLLEILMGKKPGKSG 579

BLAST of Csor.00g274510 vs. TAIR 10
Match: AT3G51740.1 (inflorescence meristem receptor-like kinase 2 )

HSP 1 Score: 152.5 bits (384), Expect = 5.8e-37
Identity = 99/290 (34.14%), Postives = 151/290 (52.07%), Query Frame = 0

Query: 62  LATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQRTQSTRLLRFL-RPGAGGGEYD- 121
           L  F+G    T D++L A  E++GKS YGT YKA+L+      + R   +   G  E++ 
Sbjct: 521 LVHFDGPFVFTADDLLCATAEIMGKSTYGTAYKATLEDGNEVAVKRLREKTTKGVKEFEG 580

Query: 122 VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESK-NWEVINKI 181
            V  LG IRH+NL+ +  +Y G +GE+LLV  +  +G+L+  +  R  E+   WE   KI
Sbjct: 581 EVTALGKIRHQNLLALRAYYLGPKGEKLLVFDYMSKGSLSAFLHARGPETLIPWETRMKI 640

Query: 182 SLGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVS 241
           + GI+R L HLH+   + + H NL + NILLD     H++D+GL  L+ AAA   ++  +
Sbjct: 641 AKGISRGLAHLHS--NENMIHENLTASNILLDEQTNAHIADYGLSRLMTAAAATNVIATA 700

Query: 242 AASGYKAPELINMTDADERTDVFSYGKILLELLSAEETG--GDGGDCGG------SEERT 301
              GY+APE   + +A  +TDV+S G I+LELL+ +  G   +G D          EE T
Sbjct: 701 GTLGYRAPEFSKIKNASAKTDVYSLGIIILELLTGKSPGEPTNGMDLPQWVASIVKEEWT 760

Query: 302 ------------------LRCLLQIGEACCSSSPDRRPDFKEVIAKIAEI 323
                             L   L++   C   SP  RP+  +V+ ++ EI
Sbjct: 761 NEVFDLELMRETQSVGDELLNTLKLALHCVDPSPAARPEANQVVEQLEEI 808

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P335434.5e-4238.21Putative kinase-like protein TMKL1 OS=Arabidopsis thaliana OX=3702 GN=TMKL1 PE=1... [more]
Q9SCT48.2e-3634.14Probably inactive leucine-rich repeat receptor-like protein kinase IMK2 OS=Arabi... [more]
Q9LVI64.5e-3432.87Probable inactive receptor kinase RLK902 OS=Arabidopsis thaliana OX=3702 GN=RLK9... [more]
Q9LP772.9e-3331.10Probable inactive receptor kinase At1g48480 OS=Arabidopsis thaliana OX=3702 GN=R... [more]
C0LGP93.8e-3330.36Probable leucine-rich repeat receptor-like protein kinase IMK3 OS=Arabidopsis th... [more]
Match NameE-valueIdentityDescription
KAG6579628.11.02e-236100.00putative kinase-like protein TMKL1, partial [Cucurbita argyrosperma subsp. soror... [more]
KAG7017083.14.12e-23499.40putative kinase-like protein TMKL1, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_022929086.12.58e-23298.79putative kinase-like protein TMKL1 [Cucurbita moschata] >XP_022929087.1 putative... [more]
XP_023520536.11.36e-22797.58putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo] >XP_023520537.1 ... [more]
XP_022969804.11.96e-22094.51putative kinase-like protein TMKL1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1ELS21.25e-23298.79putative kinase-like protein TMKL1 OS=Cucurbita moschata OX=3662 GN=LOC111435777... [more]
A0A6J1I3Q59.51e-22194.51putative kinase-like protein TMKL1 OS=Cucurbita maxima OX=3661 GN=LOC111468894 P... [more]
A0A6J1H0H84.05e-16875.07putative kinase-like protein TMKL1 OS=Cucurbita moschata OX=3662 GN=LOC111459294... [more]
A0A6J1K4E41.16e-16774.78putative kinase-like protein TMKL1 OS=Cucurbita maxima OX=3661 GN=LOC111490677 P... [more]
A0A5A7TJN44.10e-15171.22Putative kinase-like protein TMKL1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
Match NameE-valueIdentityDescription
AT5G07620.19.2e-8349.31Protein kinase superfamily protein [more]
AT5G61570.22.0e-7746.76Protein kinase superfamily protein [more]
AT5G61570.14.9e-7646.37Protein kinase superfamily protein [more]
AT3G24660.13.2e-4338.21transmembrane kinase-like 1 [more]
AT3G51740.15.8e-3734.14inflorescence meristem receptor-like kinase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 73..328
e-value: 8.0E-46
score: 158.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 44..64
NoneNo IPR availablePANTHERPTHR48008LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE IMK3-RELATEDcoord: 3..277
NoneNo IPR availablePANTHERPTHR48008LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE IMK3-RELATEDcoord: 287..323
NoneNo IPR availablePANTHERPTHR48008:SF13PROTEIN KINASE SUPERFAMILY PROTEINcoord: 287..323
coord: 3..277
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 83..275
e-value: 6.1E-22
score: 78.3
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 78..330
score: 23.665276
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 81..319

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g274510.m01Csor.00g274510.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity