Csor.00g270830 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g270830
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionNAD(P)-linked oxidoreductase superfamily protein
LocationCsor_Chr12: 14898218 .. 14902390 (-)
RNA-Seq ExpressionCsor.00g270830
SyntenyCsor.00g270830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGAGGGGCAAGGAGTTCAGATTCCCAGAGTGCAACTTGGAAGCCAGGGACTTGAGGTAAAACTCATATGCTTTTAGCTGAATGTGAGATCACATATCGGTTGAAGAGGGGAATGAAACATTCTTTATAAGGGTGTGGAAACCTCTTCCTAGATTTTAAAACCTTGACGGAAAGTCCGAAAGGGAAAGCCCAAAAAGGACAATATTTGCTAGCGGTGGGCTTGGGCTTGGGCTGTTACAAATGGTATCAGAGCCAGACACCGGGCAGTGTGCCAGCAAGAACGCGGGACTCCCTAGAGCGTAGATTGTGAGATCCCACATCGATTGGAGAAGGGAACGAAGTATTGCTTATAAGGGTGTGGAAACCTCGCCCTAGTAGACACGTTTTAAAATCTTGAGGAGAAGCCCAGGAAAAATTTAAATAGAACAATATCTGCTAGCGGTGGGCTCGGACTGTTATACGAAATGTTTATTTGCAAAACATTTCTAAACGGGATGTTAATATTCATACTTCTCTTAGTTTTAGATCATTTTCTCCATTGCTAGTCCTCCTGTCACTTTTCTTTCATATCAACAGTCATTTGATTAATATGCTTTTACACATATTCATGCTCAGTAATTCTACATTACGTGCAAGAGTACATGGAAATGTTCATGAAAAAATAACCAAACATGGCTGAAATATTCATTCTCAAGCTTAATGAAAAAAGTTCATGAACAAAAGCTTTCTCAAGCCTTATTTTCTCATATTTTTATGAACTTCAGGTCTCAAAATTGGGATTTGGATGTCTGGGCTTGACTGGAATCTACAATTCTTCTCTATCTGATGAAGATGGCATCTCAATACTAAAGGAAGCTTTCAATAAGAGAATCACTTTTTAAGATACAGCCGATACATATGGACCCCATTCTAATGAAATCCTGGTTGGAAAGGTACTCAGCTCTGCTTTTGAATGAAGGTTCCACAGCCAATAATAAGCACATAATAAACAGGTCTTTCTCTTCACTATTAACCCCTTTCCAGGCCTTGAAACAGTTACCAAGAGAAAAAGTTCAGATAGCCACAAAGTTTGGAATTACATGGGTTGGACATTCTATGACAGTGAAGGGAACCCCAGGGTATGTGCGGTCATGTTGTGAGGCTAGCTTGAAACGCCTTGACATAGACTACATTGATCTCTATTATCAACATCGTTCTGATACATCTACACCTATAGAAGAAACTGTAAATAGGCCTTACTCATTTCCCCACTTTGTTGTTCATTATACTAATTTTTTGTTCCTTTTTTGATTAGCGATAGCACTAACAAGTACTTGTAGATTGGTTTTGAGATATATGTTCTTGGTTTGTAGATGGGTGAGCTGAAAAAACTGGTGCAAGAGGGAAAGATCAAGTATATAGGTTTATCTGAAGCCAATCCACAGACAATAAGGAGAGCACATGCAGTTCATCATATTACGGCTCTACAAATGGAGTGGTCGATCTGGGCACGTGATATCGAGGACGAGATTGTTCCTCTCTGCAGGTATATGATAGACCACTGATGTATTGAAATATAAAGGACAACCGAACTACAAGATAAACCTGTTTTGAGGTACTTGAACCCTCCCTTTCTTGAGATCACTCAACCCTTCAACCAATTGAATATCAATGCTTATGCAACAGCTCAAGCCCAAGCCCATCGTTAGTAGATATTGTCATCTTTTGGCTCTCCCTTTCGGGTTTCTCCTCAAGATTTGTAAAACGTGTCTGTTTGGGAGAGGTTTCCATATCCTTATACAGAATGTTTCGTTTTCCTCCTTAACCGATGTGGAATCTCACAATCCACCCCCTTGGGGCCAGCATCCTTGCTGGCACACCACTCGGTGTTGGCTCTGATACCATTTGTAACAGCCCAAGCCCACCGCTAGCAGATGGCCCATCGTCCTCATTGACACACCACCCGGTGTCTAGTTCTGATATCATTTGTAACAGCCCAAGCCCACCGCTAGCATATATTGTCCTCTATGGGCTTACCTTTTTGGGCTTCTCCTCAAGATTTTAAAACACATCTGCAAGAGAGGTTTCCATACCCTTATAAGGAATGATTCATTCCCCTCTTCAACTGATGTGGGATCTCACACACTTGTTCCCCTCTCCAATCAATGTGGGATCTCACAATCCACCACCCCTTTGGGGCCAGCATCCACGCTGGCACTCGTTCATATCCAATCGATGTGGGATCTCAGAGCTTAACTTTCCTCTATTTATAACCAAAAACCATAACAACTATTCCTAATTTCCTTCTAATGCCATTCCTATCCGTATGATTTGATATTTTAAACCCAGATTCTCCCAACAACTGTGATTTATTAAGTTCAAAGCTAGGAACTACAGTTTGACTTTATTGTATTTGGTAGTAAGAAATAATTGTTCCATCAATGTTCAAACCTTGGACTCTAAATCTACACCTCTTGAGTGGTCCTATCCATGTTTTGCATCTGTATCTTGATCACTGGTGGTTGTCACCCATTGGTAACTGAATCTCTTCAAGGAGTAGAGAACCATAATTTCGTTACTTTGTAAGCTTCGTTTGATAACCTTTGTGACTTTTGGTTTTTTGTTTTTGAAAATTAACTGTTCTATCTATGAGTCTCTTTATTTTTTCAAAATCCAAACTAAAATTTGAAGATTAAATAGAATAGTTTGTAAAAACTTGTTTTAGTTTTTGGAGTTTGGCTAAGAATTCAAAACGTTTTCTAAGAAAAGATAAAAACATACGGTAAAGAAATGATGAGAAAACAAGTATAAATTTCAAAAACAAAGAACAAAAAACGAAAAAAATCAAATAATTATGAAATGAGACCTAAATGATTAGCTTTTGCCTTGTTACATAGCTTGTTTTTCTCGTTTTTATGCCTATGCTGCTGTCATATTTTTCCTTTGCTAAATAAGAGGACATTACCCCTTGATTGTTTTGTAGAAATCTAGGGATAGGTATTGTTCCTTACAGCCCCCTCGGTCGAGGCTTCTTTGCCGGTAAAGCCGTTGCGGAAAGTTTGCCTGCTGAGAGCCTATTGGTATCTAATTTGCACTATTTCTCTCTCAGTTAGGGTTTAATAATGCTTTGATTAGGACATTTCTTTTTTTTTTTTTTTTTTGAGACGATGGTAAGATCAGTCTGAACTAAATTTCAAAACTCGTGTCTAATCTCTTCTTTTCTTAGAGCTTACATCCTCGATTCATCAAAGAAAATTTGGAGAAGAACAAGTGCTTTTACACACGAATAGAGAAGCTTGCTGAAAAGCCCCATTGCTCTCCTGCTCAACTTTCTCTTGCATTTCTTCAACAAGGAGACGACGTTGTTCCAATTCCAAGTGATCCTCAAACCATCTTTCGGCTAATTTAGTCTCGTTCTTGTCTAAATATTAGTTGATTTGGTTAGAAGTAGTAGACCCGTGGTGAGTCAGCAAACCGGGCCTACGGTTCTAATGATTCTGTAGATACAGAAAGAGCTTCACTGATGGCTCGCTTTTCTATTTCTTTTTTCAGAAAATTGATATTAAAACGTTAACTTTAGGCTTTGTGAATTAAGGACTCATATGATTCTCCTTCCTCGACTTTGGTTCTCTCTCTTGGTTTTCAATAACATAGAAACTTGCTTGGTCAGGTTTTTCTTTTATTTGAATTCGTAGAGTAGGCAGAGACCCAACTATACGATCGGAGTTTAGACACGTAAACCATGGGTCCCCCGAATATCCATCATCAACCAACCCCCTTTCCTGGCTAAGAAGTAGAGAAGGACCTAAGAACTAAATAAACAAAGCCGTAAAAGTTTCGTGACTAAATTTATAATTTTATGGAAGTTATAGCACAAGAACAAAGATGGAAAATCTTTCATAAACCTATTATTTTGAGCTATCTTCTACCTGCTGCATACAAATTCCCGTACAGATACTTTCTTTTCTTTTAGGACTTGTTCTTGTCTTTTTTTGGTACTAGGCACAACCAAGATAAAGAATTTGGAGCAGAACATCGGTTCCTTGACGGTGAAGCTCGACAAAGACAACCTGAACGAGATTTCTGAGGCAGTACCCGAAAGCGAGGTAGCTGGAAATAGAAGTTATGATAGCATGATGCATGCAACATGGAAATATGCTAACTCACCTCCACTAGTGAATGACAACTGA

mRNA sequence

ATGGCAGAGGGGCAAGGAGTTCAGATTCCCAGAGTGCAACTTGGAAGCCAGGGACTTGAGGCCTTGAAACAGTTACCAAGAGAAAAAGTTCAGATAGCCACAAAGTTTGGAATTACATGGGTTGGACATTCTATGACAGTGAAGGGAACCCCAGGGTATGTGCGGTCATGTTGTGAGGCTAGCTTGAAACGCCTTGACATAGACTACATTGATCTCTATTATCAACATCGTTCTGATACATCTACACCTATAGAAGAAACTATGGGTGAGCTGAAAAAACTGGTGCAAGAGGGAAAGATCAAGTATATAGGTTTATCTGAAGCCAATCCACAGACAATAAGGAGAGCACATGCAGTTCATCATATTACGGCTCTACAAATGGAGTGGTCGATCTGGGCACGTGATATCGAGGACGAGATTGTTCCTCTCTGCAGAAATCTAGGGATAGGTATTGTTCCTTACAGCCCCCTCGGTCGAGGCTTCTTTGCCGGTAAAGCCGTTGCGGAAAGTTTGCCTGCTGAGAGCCTATTGAGCTTACATCCTCGATTCATCAAAGAAAATTTGGAGAAGAACAAGTGCTTTTACACACGAATAGAGAAGCTTGCTGAAAAGCCCCATTGCTCTCCTGCTCAACTTTCTCTTGCATTTCTTCAACAAGGAGACGACGTTGTTCCAATTCCAAGCACAACCAAGATAAAGAATTTGGAGCAGAACATCGGTTCCTTGACGGTGAAGCTCGACAAAGACAACCTGAACGAGATTTCTGAGGCAGTACCCGAAAGCGAGGTAGCTGGAAATAGAAGTTATGATAGCATGATGCATGCAACATGGAAATATGCTAACTCACCTCCACTAGTGAATGACAACTGA

Coding sequence (CDS)

ATGGCAGAGGGGCAAGGAGTTCAGATTCCCAGAGTGCAACTTGGAAGCCAGGGACTTGAGGCCTTGAAACAGTTACCAAGAGAAAAAGTTCAGATAGCCACAAAGTTTGGAATTACATGGGTTGGACATTCTATGACAGTGAAGGGAACCCCAGGGTATGTGCGGTCATGTTGTGAGGCTAGCTTGAAACGCCTTGACATAGACTACATTGATCTCTATTATCAACATCGTTCTGATACATCTACACCTATAGAAGAAACTATGGGTGAGCTGAAAAAACTGGTGCAAGAGGGAAAGATCAAGTATATAGGTTTATCTGAAGCCAATCCACAGACAATAAGGAGAGCACATGCAGTTCATCATATTACGGCTCTACAAATGGAGTGGTCGATCTGGGCACGTGATATCGAGGACGAGATTGTTCCTCTCTGCAGAAATCTAGGGATAGGTATTGTTCCTTACAGCCCCCTCGGTCGAGGCTTCTTTGCCGGTAAAGCCGTTGCGGAAAGTTTGCCTGCTGAGAGCCTATTGAGCTTACATCCTCGATTCATCAAAGAAAATTTGGAGAAGAACAAGTGCTTTTACACACGAATAGAGAAGCTTGCTGAAAAGCCCCATTGCTCTCCTGCTCAACTTTCTCTTGCATTTCTTCAACAAGGAGACGACGTTGTTCCAATTCCAAGCACAACCAAGATAAAGAATTTGGAGCAGAACATCGGTTCCTTGACGGTGAAGCTCGACAAAGACAACCTGAACGAGATTTCTGAGGCAGTACCCGAAAGCGAGGTAGCTGGAAATAGAAGTTATGATAGCATGATGCATGCAACATGGAAATATGCTAACTCACCTCCACTAGTGAATGACAACTGA

Protein sequence

MAEGQGVQIPRVQLGSQGLEALKQLPREKVQIATKFGITWVGHSMTVKGTPGYVRSCCEASLKRLDIDYIDLYYQHRSDTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHHITALQMEWSIWARDIEDEIVPLCRNLGIGIVPYSPLGRGFFAGKAVAESLPAESLLSLHPRFIKENLEKNKCFYTRIEKLAEKPHCSPAQLSLAFLQQGDDVVPIPSTTKIKNLEQNIGSLTVKLDKDNLNEISEAVPESEVAGNRSYDSMMHATWKYANSPPLVNDN
Homology
BLAST of Csor.00g270830 vs. ExPASy Swiss-Prot
Match: C6TBN2 (Probable aldo-keto reductase 1 OS=Glycine max OX=3847 GN=AKR1 PE=2 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 1.1e-105
Identity = 187/267 (70.04%), Postives = 220/267 (82.40%), Query Frame = 0

Query: 20  EALKQLPREKVQIATKFGITWVGH-SMTVKGTPGYVRSCCEASLKRLDIDYIDLYYQHRS 79
           +ALKQLPREK+QIATKFGI   G   M ++G+P YVRSCCE  LKRLD++YIDLYYQHR 
Sbjct: 73  KALKQLPREKIQIATKFGIASRGFPDMKIEGSPEYVRSCCETGLKRLDVEYIDLYYQHRV 132

Query: 80  DTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHHITALQMEWSIWARDIED 139
           DTS PIEET+GELKKLV+EGK+KYIGLSEA+P TIRRAHA+H ITA+Q+EWS+W RDIE+
Sbjct: 133 DTSVPIEETVGELKKLVEEGKVKYIGLSEASPDTIRRAHAIHPITAVQIEWSLWTRDIEE 192

Query: 140 EIVPLCRNLGIGIVPYSPLGRGFFAGKAVAESLPAESLLSLHPRFIKENLEKNKCFYTRI 199
           EIVPLCR LGIGIVPYSPLGRGFF GK V E++P  S L  HPRF  ENL+KNK  Y RI
Sbjct: 193 EIVPLCRELGIGIVPYSPLGRGFFGGKGVVENVPTNSSLKAHPRFQAENLDKNKNIYERI 252

Query: 200 EKLAEKPHCSPAQLSLAF-LQQGDDVVPIPSTTKIKNLEQNIGSLTVKLDKDNLNEISEA 259
           E LA+K   +PAQL+LA+ LQQG+DVVPIP TTKIKNL+QNIG+L VKL + +L EI EA
Sbjct: 253 EGLAKKHQATPAQLALAWVLQQGEDVVPIPGTTKIKNLDQNIGALAVKLSEKDLREIFEA 312

Query: 260 VPESEVAGNRSYDSMMHATWKYANSPP 285
           VP  +VAG R Y+ + H +WKYAN+PP
Sbjct: 313 VPIGDVAGGRYYNGLDHFSWKYANTPP 339

BLAST of Csor.00g270830 vs. ExPASy Swiss-Prot
Match: Q3L181 (Perakine reductase OS=Rauvolfia serpentina OX=4060 GN=PR PE=1 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 2.1e-104
Identity = 185/268 (69.03%), Postives = 221/268 (82.46%), Query Frame = 0

Query: 20  EALKQLPREKVQIATKFGITWVGHS-MTVKGTPGYVRSCCEASLKRLDIDYIDLYYQHRS 79
           +ALKQLPREK+Q+ TKFGI  +G S +  KGTP YVRSCCEASLKRLD+DYIDL+Y HR 
Sbjct: 69  KALKQLPREKIQVGTKFGIHEIGFSGVKAKGTPDYVRSCCEASLKRLDVDYIDLFYIHRI 128

Query: 80  DTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHHITALQMEWSIWARDIED 139
           DT+ PIE TMGELKKLV+EGKIKY+GLSEA+P TIRRAHAVH +TALQ+E+S+W RDIED
Sbjct: 129 DTTVPIEITMGELKKLVEEGKIKYVGLSEASPDTIRRAHAVHPVTALQIEYSLWTRDIED 188

Query: 140 EIVPLCRNLGIGIVPYSPLGRGFFAGKAVAESLPAESLLSLHPRFIKENLEKNKCFYTRI 199
           EIVPLCR LGIGIVPYSP+GRG FAGKA+ ESLP  S+L+ HPRF+ ENLEKNK  Y RI
Sbjct: 189 EIVPLCRQLGIGIVPYSPIGRGLFAGKAIKESLPENSVLTSHPRFVGENLEKNKQIYYRI 248

Query: 200 EKLAEKPHCSPAQLSLAF-LQQGDDVVPIPSTTKIKNLEQNIGSLTVKLDKDNLNEISEA 259
           E L++K  C+P QL+LA+ L QG+DVVPIP TTKIKNL  N+G+L VKL K++L EIS+A
Sbjct: 249 EALSQKHGCTPVQLALAWVLHQGEDVVPIPGTTKIKNLHNNVGALKVKLTKEDLKEISDA 308

Query: 260 VPESEVAGNRSYDSMMHATWKYANSPPL 286
           VP  EVAG   ++ +    WK+AN+PPL
Sbjct: 309 VPLDEVAGESIHEVIAVTNWKFANTPPL 336

BLAST of Csor.00g270830 vs. ExPASy Swiss-Prot
Match: Q93ZN2 (Probable aldo-keto reductase 4 OS=Arabidopsis thaliana OX=3702 GN=At1g60710 PE=2 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 3.9e-82
Identity = 158/268 (58.96%), Postives = 198/268 (73.88%), Query Frame = 0

Query: 20  EALKQLPREKVQIATKFGITWVGHSMTVKGTPGYVRSCCEASLKRLDIDYIDLYYQHRSD 79
           +ALK   REKV++ATKFGI++      V+G P YVR+ CEASLKRLDI  IDLYYQHR D
Sbjct: 74  KALKDGVREKVELATKFGISYAEGKREVRGDPEYVRAACEASLKRLDIACIDLYYQHRVD 133

Query: 80  TSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHHITALQMEWSIWARDIEDE 139
           T  PIE TMGELKKLV+EGKIKYIGLSEA+  TIRRAHAVH ITA+Q+EWS+W RD+E+E
Sbjct: 134 TRVPIEITMGELKKLVEEGKIKYIGLSEASASTIRRAHAVHPITAVQIEWSLWTRDVEEE 193

Query: 140 IVPLCRNLGIGIVPYSPLGRGFFA-GKAVAESLPAESLLSLHPRFIKENLEKNKCFYTRI 199
           I+P CR LGIGIV YSPLGRGFFA G  + E+L  +      PRF +ENL+ NK  Y ++
Sbjct: 194 IIPTCRELGIGIVAYSPLGRGFFASGPKLVENLEKDDFRKALPRFQEENLDHNKIVYEKV 253

Query: 200 EKLAEKPHCSPAQLSLAFL-QQGDDVVPIPSTTKIKNLEQNIGSLTVKLDKDNLNEISEA 259
             ++EK  C+P QL+LA++  QGDDV PIP TTKI+NL+QNIG+L+VKL  + + E+   
Sbjct: 254 CAISEKKGCTPGQLALAWVHHQGDDVCPIPGTTKIENLKQNIGALSVKLTPEEMTELEAI 313

Query: 260 VPESEVAGNRSYDSMMHATWKYANSPPL 286
                V G+R Y +M+  T+K A +PPL
Sbjct: 314 AQPGFVKGDR-YSNMI-PTFKNAETPPL 339

BLAST of Csor.00g270830 vs. ExPASy Swiss-Prot
Match: O22707 (Probable aldo-keto reductase 3 OS=Arabidopsis thaliana OX=3702 GN=At1g60690 PE=3 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 1.9e-81
Identity = 154/272 (56.62%), Postives = 197/272 (72.43%), Query Frame = 0

Query: 20  EALKQLPREKVQIATKFGITWVGHSMTVKGTPGYVRSCCEASLKRLDIDYIDLYYQHRSD 79
           +ALK   REKV++ATKFGI++   +  +KG P YVR+ CEASLKRLD+  IDLYYQHR D
Sbjct: 74  KALKDGVREKVELATKFGISYAEGNREIKGDPAYVRAACEASLKRLDVTCIDLYYQHRID 133

Query: 80  TSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHHITALQMEWSIWARDIEDE 139
           T  PIE TMGELKKL++EGKIKYIGLSEA+  TIRRAH VH ITA+Q+EWS+W RD+E+E
Sbjct: 134 TRVPIEITMGELKKLIEEGKIKYIGLSEASASTIRRAHTVHPITAVQLEWSLWTRDVEEE 193

Query: 140 IVPLCRNLGIGIVPYSPLGRGFFA-GKAVAESLPAESLLSLHPRFIKENLEKNKCFYTRI 199
           IVP CR LGIGIV YSPLGRGFFA G  + E+L         PRF +ENL+ NK  Y ++
Sbjct: 194 IVPTCRELGIGIVSYSPLGRGFFASGPKLVENLDNNDFRKALPRFQQENLDHNKILYEKV 253

Query: 200 EKLAEKPHCSPAQLSLAFL-QQGDDVVPIPSTTKIKNLEQNIGSLTVKLDKDNLNEISEA 259
             ++EK  C+PAQL+LA++  QGDDV PIP TTKI+NL QNI +L+VKL  + ++E+   
Sbjct: 254 SAMSEKKGCTPAQLALAWVHHQGDDVCPIPGTTKIENLNQNIRALSVKLTPEEMSELETI 313

Query: 260 VPESEVAGNRSYDSMMHATWKYANSPPLVNDN 290
                V G R   ++   T+K +++PPL + N
Sbjct: 314 AQPESVKGERYMATV--PTFKNSDTPPLSSWN 343

BLAST of Csor.00g270830 vs. ExPASy Swiss-Prot
Match: Q9ASZ9 (Probable aldo-keto reductase 5 OS=Arabidopsis thaliana OX=3702 GN=At1g60730 PE=2 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 2.1e-80
Identity = 151/268 (56.34%), Postives = 195/268 (72.76%), Query Frame = 0

Query: 20  EALKQLPREKVQIATKFGITWVGHSMTVKGTPGYVRSCCEASLKRLDIDYIDLYYQHRSD 79
           +ALK   REKV++ATK+GI +    +  KG P YVR+ CEASL R+D+  IDLYYQHR D
Sbjct: 74  KALKDGVREKVELATKYGIRYAEGKVEFKGDPAYVRAACEASLMRVDVACIDLYYQHRID 133

Query: 80  TSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHHITALQMEWSIWARDIEDE 139
           T  PIE T+GELKKLV+EGKIKYIGLSEA+  TIRRAHAVH ITALQ+EWS+W+RD+E++
Sbjct: 134 TRVPIEITIGELKKLVEEGKIKYIGLSEASASTIRRAHAVHPITALQIEWSLWSRDVEED 193

Query: 140 IVPLCRNLGIGIVPYSPLGRGFFA-GKAVAESLPAESLLSLHPRFIKENLEKNKCFYTRI 199
           I+P CR LGIGIV YSPLGRGFFA G  + E+L    +    PRF +ENL+ NK  + ++
Sbjct: 194 IIPTCRELGIGIVAYSPLGRGFFASGPKLVENLDNNDVRKTLPRFQQENLDHNKILFEKV 253

Query: 200 EKLAEKPHCSPAQLSLAFL-QQGDDVVPIPSTTKIKNLEQNIGSLTVKLDKDNLNEISEA 259
             ++EK  C+PAQL+LA++  QGDDV PIP TTKI+NL QNIG+L+VKL  + ++E+   
Sbjct: 254 SAMSEKKGCTPAQLALAWVHHQGDDVCPIPGTTKIENLNQNIGALSVKLTPEEMSELESL 313

Query: 260 VPESEVAGNRSYDSMMHATWKYANSPPL 286
                V G RS   +   T+K + +PPL
Sbjct: 314 AQPGFVKGERSISIL--TTFKNSETPPL 339

BLAST of Csor.00g270830 vs. NCBI nr
Match: KAG6586306.1 (putative aldo-keto reductase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 585 bits (1508), Expect = 8.12e-211
Identity = 289/289 (100.00%), Postives = 289/289 (100.00%), Query Frame = 0

Query: 1   MAEGQGVQIPRVQLGSQGLEALKQLPREKVQIATKFGITWVGHSMTVKGTPGYVRSCCEA 60
           MAEGQGVQIPRVQLGSQGLEALKQLPREKVQIATKFGITWVGHSMTVKGTPGYVRSCCEA
Sbjct: 1   MAEGQGVQIPRVQLGSQGLEALKQLPREKVQIATKFGITWVGHSMTVKGTPGYVRSCCEA 60

Query: 61  SLKRLDIDYIDLYYQHRSDTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVH 120
           SLKRLDIDYIDLYYQHRSDTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVH
Sbjct: 61  SLKRLDIDYIDLYYQHRSDTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVH 120

Query: 121 HITALQMEWSIWARDIEDEIVPLCRNLGIGIVPYSPLGRGFFAGKAVAESLPAESLLSLH 180
           HITALQMEWSIWARDIEDEIVPLCRNLGIGIVPYSPLGRGFFAGKAVAESLPAESLLSLH
Sbjct: 121 HITALQMEWSIWARDIEDEIVPLCRNLGIGIVPYSPLGRGFFAGKAVAESLPAESLLSLH 180

Query: 181 PRFIKENLEKNKCFYTRIEKLAEKPHCSPAQLSLAFLQQGDDVVPIPSTTKIKNLEQNIG 240
           PRFIKENLEKNKCFYTRIEKLAEKPHCSPAQLSLAFLQQGDDVVPIPSTTKIKNLEQNIG
Sbjct: 181 PRFIKENLEKNKCFYTRIEKLAEKPHCSPAQLSLAFLQQGDDVVPIPSTTKIKNLEQNIG 240

Query: 241 SLTVKLDKDNLNEISEAVPESEVAGNRSYDSMMHATWKYANSPPLVNDN 289
           SLTVKLDKDNLNEISEAVPESEVAGNRSYDSMMHATWKYANSPPLVNDN
Sbjct: 241 SLTVKLDKDNLNEISEAVPESEVAGNRSYDSMMHATWKYANSPPLVNDN 289

BLAST of Csor.00g270830 vs. NCBI nr
Match: XP_023537103.1 (perakine reductase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 525 bits (1352), Expect = 3.92e-186
Identity = 274/345 (79.42%), Postives = 282/345 (81.74%), Query Frame = 0

Query: 1   MAEGQGVQIPRVQLGSQGLE---------------------------------------- 60
           MAEGQGVQIPRVQLGSQGLE                                        
Sbjct: 1   MAEGQGVQIPRVQLGSQGLEVSKLGFGCLGLTGIYNSSLSDEDGISILKEAFNKGITFFD 60

Query: 61  ----------------ALKQLPREKVQIATKFGITWVGHSMTVKGTPGYVRSCCEASLKR 120
                           ALKQLPREKVQIATKFGITWVGHSMTVKGTPGYVRSCCEASLKR
Sbjct: 61  TADSYGPHSNEILIGKALKQLPREKVQIATKFGITWVGHSMTVKGTPGYVRSCCEASLKR 120

Query: 121 LDIDYIDLYYQHRSDTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHHITA 180
           LDIDYIDLYYQHRSDTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVH ITA
Sbjct: 121 LDIDYIDLYYQHRSDTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHPITA 180

Query: 181 LQMEWSIWARDIEDEIVPLCRNLGIGIVPYSPLGRGFFAGKAVAESLPAESLLSLHPRFI 240
           LQMEWSIW RDIEDEIVPLCRNLGIGIVPYSPLGRGFFAGKAVAESLPAESLLSLHPRFI
Sbjct: 181 LQMEWSIWTRDIEDEIVPLCRNLGIGIVPYSPLGRGFFAGKAVAESLPAESLLSLHPRFI 240

Query: 241 KENLEKNKCFYTRIEKLAEKPHCSPAQLSLAF-LQQGDDVVPIPSTTKIKNLEQNIGSLT 288
           +ENLEKNKCFYTRIEKLAEK HCSPAQLSLA+ LQQGDDVVPIP TTKIKNLEQNIGSLT
Sbjct: 241 EENLEKNKCFYTRIEKLAEKHHCSPAQLSLAWVLQQGDDVVPIPGTTKIKNLEQNIGSLT 300

BLAST of Csor.00g270830 vs. NCBI nr
Match: XP_023537102.1 (probable aldo-keto reductase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 519 bits (1336), Expect = 1.07e-183
Identity = 273/345 (79.13%), Postives = 280/345 (81.16%), Query Frame = 0

Query: 1   MAEGQGVQIPRVQLGSQGLE---------------------------------------- 60
           MAEGQGVQIPRVQLGSQGLE                                        
Sbjct: 1   MAEGQGVQIPRVQLGSQGLEVSKLGFGCMGLTGIYNSSLSDEEGISILKEAFNKGITFFD 60

Query: 61  ----------------ALKQLPREKVQIATKFGITWVGHSMTVKGTPGYVRSCCEASLKR 120
                           ALKQLPREKVQIATKFGIT VGHSMTVKGTP YVR+CCEASLKR
Sbjct: 61  TADAYGPHSNEILIGKALKQLPREKVQIATKFGITRVGHSMTVKGTPEYVRACCEASLKR 120

Query: 121 LDIDYIDLYYQHRSDTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHHITA 180
           LDIDYIDLYYQHR+DTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVH ITA
Sbjct: 121 LDIDYIDLYYQHRTDTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHPITA 180

Query: 181 LQMEWSIWARDIEDEIVPLCRNLGIGIVPYSPLGRGFFAGKAVAESLPAESLLSLHPRFI 240
           LQMEWSIW RDIEDEIVPLCR+LGIGIVPYSPLGRGFFAGKAV ESLP+ES LSLHPRFI
Sbjct: 181 LQMEWSIWTRDIEDEIVPLCRDLGIGIVPYSPLGRGFFAGKAVVESLPSESQLSLHPRFI 240

Query: 241 KENLEKNKCFYTRIEKLAEKPHCSPAQLSLAF-LQQGDDVVPIPSTTKIKNLEQNIGSLT 288
           +ENLEKNKCFYTRIEKLAEK HCSPAQLSLA+ LQQGDDVVPIP TTKIKNLEQNIGSLT
Sbjct: 241 EENLEKNKCFYTRIEKLAEKHHCSPAQLSLAWVLQQGDDVVPIPGTTKIKNLEQNIGSLT 300

BLAST of Csor.00g270830 vs. NCBI nr
Match: XP_022937674.1 (perakine reductase-like isoform X1 [Cucurbita moschata] >XP_022937675.1 perakine reductase-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 514 bits (1325), Expect = 5.08e-182
Identity = 270/345 (78.26%), Postives = 278/345 (80.58%), Query Frame = 0

Query: 1   MAEGQGVQIPRVQLGSQGLE---------------------------------------- 60
           MAEGQGVQIPRVQLGSQGLE                                        
Sbjct: 1   MAEGQGVQIPRVQLGSQGLEVSKLGFGCMGLTGIYNSSLSDEEGISILKEAFNKGITFFD 60

Query: 61  ----------------ALKQLPREKVQIATKFGITWVGHSMTVKGTPGYVRSCCEASLKR 120
                           ALKQLPREKVQIATKFG+T VGHSMTVKGTP YVR+CCEASLKR
Sbjct: 61  TADAYGPHSNETLIGKALKQLPREKVQIATKFGVTRVGHSMTVKGTPEYVRACCEASLKR 120

Query: 121 LDIDYIDLYYQHRSDTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHHITA 180
           LDIDYIDLYYQHR+DTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVH ITA
Sbjct: 121 LDIDYIDLYYQHRTDTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHPITA 180

Query: 181 LQMEWSIWARDIEDEIVPLCRNLGIGIVPYSPLGRGFFAGKAVAESLPAESLLSLHPRFI 240
           LQMEWSIW RDIEDEIVPLCR+LGIGIVPYSPLGRGFFAGKAV ESLP ES LSLHPRFI
Sbjct: 181 LQMEWSIWTRDIEDEIVPLCRDLGIGIVPYSPLGRGFFAGKAVVESLPTESQLSLHPRFI 240

Query: 241 KENLEKNKCFYTRIEKLAEKPHCSPAQLSLAF-LQQGDDVVPIPSTTKIKNLEQNIGSLT 288
           +ENLEKNKCFYTRIEKLAEK HCSPAQLSLA+ LQQGDDVVPIP TTKIKNLE NIGSLT
Sbjct: 241 EENLEKNKCFYTRIEKLAEKHHCSPAQLSLAWVLQQGDDVVPIPGTTKIKNLEHNIGSLT 300

BLAST of Csor.00g270830 vs. NCBI nr
Match: XP_022965820.1 (probable aldo-keto reductase 1 isoform X1 [Cucurbita maxima] >XP_022965821.1 probable aldo-keto reductase 1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 514 bits (1323), Expect = 1.02e-181
Identity = 267/345 (77.39%), Postives = 280/345 (81.16%), Query Frame = 0

Query: 1   MAEGQGVQIPRVQLGSQGLE---------------------------------------- 60
           MA GQGVQIP+V+LGSQGLE                                        
Sbjct: 1   MAGGQGVQIPKVRLGSQGLEVSKLGFGCMGLTGIYNSSLSDEEGISILKGAFNKGITFFD 60

Query: 61  ----------------ALKQLPREKVQIATKFGITWVGHSMTVKGTPGYVRSCCEASLKR 120
                           ALKQLPREKVQIATKFGITWVGHSMTVKGTP YVRSCCEASLKR
Sbjct: 61  TADAYGPHSNEILIGKALKQLPREKVQIATKFGITWVGHSMTVKGTPEYVRSCCEASLKR 120

Query: 121 LDIDYIDLYYQHRSDTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHHITA 180
           LDIDYIDLYYQHR+DTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVH ITA
Sbjct: 121 LDIDYIDLYYQHRTDTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHPITA 180

Query: 181 LQMEWSIWARDIEDEIVPLCRNLGIGIVPYSPLGRGFFAGKAVAESLPAESLLSLHPRFI 240
           LQMEWSIW RDIEDEIVPLCR+LGIGIVPYSPLGRGFFAGKAV ESLPAESLLSLHPRFI
Sbjct: 181 LQMEWSIWTRDIEDEIVPLCRDLGIGIVPYSPLGRGFFAGKAVVESLPAESLLSLHPRFI 240

Query: 241 KENLEKNKCFYTRIEKLAEKPHCSPAQLSLAF-LQQGDDVVPIPSTTKIKNLEQNIGSLT 288
           +ENLEKNKCFYTRIE+LAEK HCSPAQLSLA+ LQQGDDVVPIP TTKIKNLEQNIGSLT
Sbjct: 241 EENLEKNKCFYTRIERLAEKHHCSPAQLSLAWVLQQGDDVVPIPGTTKIKNLEQNIGSLT 300

BLAST of Csor.00g270830 vs. ExPASy TrEMBL
Match: A0A6J1FBV8 (perakine reductase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444009 PE=4 SV=1)

HSP 1 Score: 514 bits (1325), Expect = 2.46e-182
Identity = 270/345 (78.26%), Postives = 278/345 (80.58%), Query Frame = 0

Query: 1   MAEGQGVQIPRVQLGSQGLE---------------------------------------- 60
           MAEGQGVQIPRVQLGSQGLE                                        
Sbjct: 1   MAEGQGVQIPRVQLGSQGLEVSKLGFGCMGLTGIYNSSLSDEEGISILKEAFNKGITFFD 60

Query: 61  ----------------ALKQLPREKVQIATKFGITWVGHSMTVKGTPGYVRSCCEASLKR 120
                           ALKQLPREKVQIATKFG+T VGHSMTVKGTP YVR+CCEASLKR
Sbjct: 61  TADAYGPHSNETLIGKALKQLPREKVQIATKFGVTRVGHSMTVKGTPEYVRACCEASLKR 120

Query: 121 LDIDYIDLYYQHRSDTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHHITA 180
           LDIDYIDLYYQHR+DTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVH ITA
Sbjct: 121 LDIDYIDLYYQHRTDTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHPITA 180

Query: 181 LQMEWSIWARDIEDEIVPLCRNLGIGIVPYSPLGRGFFAGKAVAESLPAESLLSLHPRFI 240
           LQMEWSIW RDIEDEIVPLCR+LGIGIVPYSPLGRGFFAGKAV ESLP ES LSLHPRFI
Sbjct: 181 LQMEWSIWTRDIEDEIVPLCRDLGIGIVPYSPLGRGFFAGKAVVESLPTESQLSLHPRFI 240

Query: 241 KENLEKNKCFYTRIEKLAEKPHCSPAQLSLAF-LQQGDDVVPIPSTTKIKNLEQNIGSLT 288
           +ENLEKNKCFYTRIEKLAEK HCSPAQLSLA+ LQQGDDVVPIP TTKIKNLE NIGSLT
Sbjct: 241 EENLEKNKCFYTRIEKLAEKHHCSPAQLSLAWVLQQGDDVVPIPGTTKIKNLEHNIGSLT 300

BLAST of Csor.00g270830 vs. ExPASy TrEMBL
Match: A0A6J1HPU0 (probable aldo-keto reductase 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465593 PE=4 SV=1)

HSP 1 Score: 514 bits (1323), Expect = 4.96e-182
Identity = 267/345 (77.39%), Postives = 280/345 (81.16%), Query Frame = 0

Query: 1   MAEGQGVQIPRVQLGSQGLE---------------------------------------- 60
           MA GQGVQIP+V+LGSQGLE                                        
Sbjct: 1   MAGGQGVQIPKVRLGSQGLEVSKLGFGCMGLTGIYNSSLSDEEGISILKGAFNKGITFFD 60

Query: 61  ----------------ALKQLPREKVQIATKFGITWVGHSMTVKGTPGYVRSCCEASLKR 120
                           ALKQLPREKVQIATKFGITWVGHSMTVKGTP YVRSCCEASLKR
Sbjct: 61  TADAYGPHSNEILIGKALKQLPREKVQIATKFGITWVGHSMTVKGTPEYVRSCCEASLKR 120

Query: 121 LDIDYIDLYYQHRSDTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHHITA 180
           LDIDYIDLYYQHR+DTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVH ITA
Sbjct: 121 LDIDYIDLYYQHRTDTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHPITA 180

Query: 181 LQMEWSIWARDIEDEIVPLCRNLGIGIVPYSPLGRGFFAGKAVAESLPAESLLSLHPRFI 240
           LQMEWSIW RDIEDEIVPLCR+LGIGIVPYSPLGRGFFAGKAV ESLPAESLLSLHPRFI
Sbjct: 181 LQMEWSIWTRDIEDEIVPLCRDLGIGIVPYSPLGRGFFAGKAVVESLPAESLLSLHPRFI 240

Query: 241 KENLEKNKCFYTRIEKLAEKPHCSPAQLSLAF-LQQGDDVVPIPSTTKIKNLEQNIGSLT 288
           +ENLEKNKCFYTRIE+LAEK HCSPAQLSLA+ LQQGDDVVPIP TTKIKNLEQNIGSLT
Sbjct: 241 EENLEKNKCFYTRIERLAEKHHCSPAQLSLAWVLQQGDDVVPIPGTTKIKNLEQNIGSLT 300

BLAST of Csor.00g270830 vs. ExPASy TrEMBL
Match: A0A6J1HQ36 (probable aldo-keto reductase 1 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111465593 PE=4 SV=1)

HSP 1 Score: 508 bits (1309), Expect = 2.43e-180
Identity = 250/270 (92.59%), Postives = 262/270 (97.04%), Query Frame = 0

Query: 20  EALKQLPREKVQIATKFGITWVGHSMTVKGTPGYVRSCCEASLKRLDIDYIDLYYQHRSD 79
           +ALKQLPREKVQIATKFGITWVGHSMTVKGTP YVRSCCEASLKRLDIDYIDLYYQHR+D
Sbjct: 48  KALKQLPREKVQIATKFGITWVGHSMTVKGTPEYVRSCCEASLKRLDIDYIDLYYQHRTD 107

Query: 80  TSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHHITALQMEWSIWARDIEDE 139
           TSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVH ITALQMEWSIW RDIEDE
Sbjct: 108 TSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHPITALQMEWSIWTRDIEDE 167

Query: 140 IVPLCRNLGIGIVPYSPLGRGFFAGKAVAESLPAESLLSLHPRFIKENLEKNKCFYTRIE 199
           IVPLCR+LGIGIVPYSPLGRGFFAGKAV ESLPAESLLSLHPRFI+ENLEKNKCFYTRIE
Sbjct: 168 IVPLCRDLGIGIVPYSPLGRGFFAGKAVVESLPAESLLSLHPRFIEENLEKNKCFYTRIE 227

Query: 200 KLAEKPHCSPAQLSLAF-LQQGDDVVPIPSTTKIKNLEQNIGSLTVKLDKDNLNEISEAV 259
           +LAEK HCSPAQLSLA+ LQQGDDVVPIP TTKIKNLEQNIGSLTVKLDKD+LNEISEAV
Sbjct: 228 RLAEKHHCSPAQLSLAWVLQQGDDVVPIPGTTKIKNLEQNIGSLTVKLDKDDLNEISEAV 287

Query: 260 PESEVAGNRSYDSMMHATWKYANSPPLVND 288
           PES+VAGNR+YDS+MHATWKYAN+PP +ND
Sbjct: 288 PESKVAGNRNYDSLMHATWKYANTPPKMND 317

BLAST of Csor.00g270830 vs. ExPASy TrEMBL
Match: A0A6J1HS01 (probable aldo-keto reductase 1 OS=Cucurbita maxima OX=3661 GN=LOC111465592 PE=4 SV=1)

HSP 1 Score: 505 bits (1301), Expect = 1.11e-178
Identity = 264/345 (76.52%), Postives = 277/345 (80.29%), Query Frame = 0

Query: 1   MAEGQGVQIPRVQLGSQGLE---------------------------------------- 60
           MA GQGVQIP+V+LGSQGLE                                        
Sbjct: 1   MAGGQGVQIPKVRLGSQGLEVSKLGFGCMGLTGIYNSSLSDEEGISILKGAFNKGITFFD 60

Query: 61  ----------------ALKQLPREKVQIATKFGITWVGHSMTVKGTPGYVRSCCEASLKR 120
                           ALKQLPREKVQIATKFGIT VGHSMTV+GTP YVR+CCEASLKR
Sbjct: 61  TADAYGPHSNEILIGKALKQLPREKVQIATKFGITRVGHSMTVQGTPEYVRACCEASLKR 120

Query: 121 LDIDYIDLYYQHRSDTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHHITA 180
           LDIDYIDLYYQHR+DTSTPIEETMGELKKLVQEGKIKY+GLSEANPQTIRRAHAVH ITA
Sbjct: 121 LDIDYIDLYYQHRTDTSTPIEETMGELKKLVQEGKIKYVGLSEANPQTIRRAHAVHPITA 180

Query: 181 LQMEWSIWARDIEDEIVPLCRNLGIGIVPYSPLGRGFFAGKAVAESLPAESLLSLHPRFI 240
           LQMEWSIW RDIEDEI+PLCR+LGIGIVPYSPLGRGFFAGKAV ESLPAES LSLHPRFI
Sbjct: 181 LQMEWSIWTRDIEDEIIPLCRDLGIGIVPYSPLGRGFFAGKAVVESLPAESQLSLHPRFI 240

Query: 241 KENLEKNKCFYTRIEKLAEKPHCSPAQLSLAF-LQQGDDVVPIPSTTKIKNLEQNIGSLT 288
           +ENLEKNKCFYTRIE+LAEK HCSPAQLSLA+ LQQGDDVVPIP TTKIKNLE NIGSLT
Sbjct: 241 EENLEKNKCFYTRIERLAEKHHCSPAQLSLAWVLQQGDDVVPIPGTTKIKNLEHNIGSLT 300

BLAST of Csor.00g270830 vs. ExPASy TrEMBL
Match: A0A6J1FB17 (probable aldo-keto reductase 1 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111444009 PE=4 SV=1)

HSP 1 Score: 504 bits (1297), Expect = 1.64e-178
Identity = 250/270 (92.59%), Postives = 259/270 (95.93%), Query Frame = 0

Query: 20  EALKQLPREKVQIATKFGITWVGHSMTVKGTPGYVRSCCEASLKRLDIDYIDLYYQHRSD 79
           +ALKQLPREKVQIATKFG+T VGHSMTVKGTP YVR+CCEASLKRLDIDYIDLYYQHR+D
Sbjct: 48  KALKQLPREKVQIATKFGVTRVGHSMTVKGTPEYVRACCEASLKRLDIDYIDLYYQHRTD 107

Query: 80  TSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHHITALQMEWSIWARDIEDE 139
           TSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVH ITALQMEWSIW RDIEDE
Sbjct: 108 TSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHPITALQMEWSIWTRDIEDE 167

Query: 140 IVPLCRNLGIGIVPYSPLGRGFFAGKAVAESLPAESLLSLHPRFIKENLEKNKCFYTRIE 199
           IVPLCR+LGIGIVPYSPLGRGFFAGKAV ESLP ES LSLHPRFI+ENLEKNKCFYTRIE
Sbjct: 168 IVPLCRDLGIGIVPYSPLGRGFFAGKAVVESLPTESQLSLHPRFIEENLEKNKCFYTRIE 227

Query: 200 KLAEKPHCSPAQLSLAF-LQQGDDVVPIPSTTKIKNLEQNIGSLTVKLDKDNLNEISEAV 259
           KLAEK HCSPAQLSLA+ LQQGDDVVPIP TTKIKNLE NIGSLTVKLDKD+LNEISEAV
Sbjct: 228 KLAEKHHCSPAQLSLAWVLQQGDDVVPIPGTTKIKNLEHNIGSLTVKLDKDDLNEISEAV 287

Query: 260 PESEVAGNRSYDSMMHATWKYANSPPLVND 288
           PESEVAGNRSYDSM+HATWKYANSPPLVND
Sbjct: 288 PESEVAGNRSYDSMIHATWKYANSPPLVND 317

BLAST of Csor.00g270830 vs. TAIR 10
Match: AT1G60710.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 306.2 bits (783), Expect = 2.8e-83
Identity = 158/268 (58.96%), Postives = 198/268 (73.88%), Query Frame = 0

Query: 20  EALKQLPREKVQIATKFGITWVGHSMTVKGTPGYVRSCCEASLKRLDIDYIDLYYQHRSD 79
           +ALK   REKV++ATKFGI++      V+G P YVR+ CEASLKRLDI  IDLYYQHR D
Sbjct: 74  KALKDGVREKVELATKFGISYAEGKREVRGDPEYVRAACEASLKRLDIACIDLYYQHRVD 133

Query: 80  TSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHHITALQMEWSIWARDIEDE 139
           T  PIE TMGELKKLV+EGKIKYIGLSEA+  TIRRAHAVH ITA+Q+EWS+W RD+E+E
Sbjct: 134 TRVPIEITMGELKKLVEEGKIKYIGLSEASASTIRRAHAVHPITAVQIEWSLWTRDVEEE 193

Query: 140 IVPLCRNLGIGIVPYSPLGRGFFA-GKAVAESLPAESLLSLHPRFIKENLEKNKCFYTRI 199
           I+P CR LGIGIV YSPLGRGFFA G  + E+L  +      PRF +ENL+ NK  Y ++
Sbjct: 194 IIPTCRELGIGIVAYSPLGRGFFASGPKLVENLEKDDFRKALPRFQEENLDHNKIVYEKV 253

Query: 200 EKLAEKPHCSPAQLSLAFL-QQGDDVVPIPSTTKIKNLEQNIGSLTVKLDKDNLNEISEA 259
             ++EK  C+P QL+LA++  QGDDV PIP TTKI+NL+QNIG+L+VKL  + + E+   
Sbjct: 254 CAISEKKGCTPGQLALAWVHHQGDDVCPIPGTTKIENLKQNIGALSVKLTPEEMTELEAI 313

Query: 260 VPESEVAGNRSYDSMMHATWKYANSPPL 286
                V G+R Y +M+  T+K A +PPL
Sbjct: 314 AQPGFVKGDR-YSNMI-PTFKNAETPPL 339

BLAST of Csor.00g270830 vs. TAIR 10
Match: AT1G60690.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 303.9 bits (777), Expect = 1.4e-82
Identity = 154/272 (56.62%), Postives = 197/272 (72.43%), Query Frame = 0

Query: 20  EALKQLPREKVQIATKFGITWVGHSMTVKGTPGYVRSCCEASLKRLDIDYIDLYYQHRSD 79
           +ALK   REKV++ATKFGI++   +  +KG P YVR+ CEASLKRLD+  IDLYYQHR D
Sbjct: 74  KALKDGVREKVELATKFGISYAEGNREIKGDPAYVRAACEASLKRLDVTCIDLYYQHRID 133

Query: 80  TSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHHITALQMEWSIWARDIEDE 139
           T  PIE TMGELKKL++EGKIKYIGLSEA+  TIRRAH VH ITA+Q+EWS+W RD+E+E
Sbjct: 134 TRVPIEITMGELKKLIEEGKIKYIGLSEASASTIRRAHTVHPITAVQLEWSLWTRDVEEE 193

Query: 140 IVPLCRNLGIGIVPYSPLGRGFFA-GKAVAESLPAESLLSLHPRFIKENLEKNKCFYTRI 199
           IVP CR LGIGIV YSPLGRGFFA G  + E+L         PRF +ENL+ NK  Y ++
Sbjct: 194 IVPTCRELGIGIVSYSPLGRGFFASGPKLVENLDNNDFRKALPRFQQENLDHNKILYEKV 253

Query: 200 EKLAEKPHCSPAQLSLAFL-QQGDDVVPIPSTTKIKNLEQNIGSLTVKLDKDNLNEISEA 259
             ++EK  C+PAQL+LA++  QGDDV PIP TTKI+NL QNI +L+VKL  + ++E+   
Sbjct: 254 SAMSEKKGCTPAQLALAWVHHQGDDVCPIPGTTKIENLNQNIRALSVKLTPEEMSELETI 313

Query: 260 VPESEVAGNRSYDSMMHATWKYANSPPLVNDN 290
                V G R   ++   T+K +++PPL + N
Sbjct: 314 AQPESVKGERYMATV--PTFKNSDTPPLSSWN 343

BLAST of Csor.00g270830 vs. TAIR 10
Match: AT1G60730.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 300.4 bits (768), Expect = 1.5e-81
Identity = 151/268 (56.34%), Postives = 195/268 (72.76%), Query Frame = 0

Query: 20  EALKQLPREKVQIATKFGITWVGHSMTVKGTPGYVRSCCEASLKRLDIDYIDLYYQHRSD 79
           +ALK   REKV++ATK+GI +    +  KG P YVR+ CEASL R+D+  IDLYYQHR D
Sbjct: 74  KALKDGVREKVELATKYGIRYAEGKVEFKGDPAYVRAACEASLMRVDVACIDLYYQHRID 133

Query: 80  TSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHHITALQMEWSIWARDIEDE 139
           T  PIE T+GELKKLV+EGKIKYIGLSEA+  TIRRAHAVH ITALQ+EWS+W+RD+E++
Sbjct: 134 TRVPIEITIGELKKLVEEGKIKYIGLSEASASTIRRAHAVHPITALQIEWSLWSRDVEED 193

Query: 140 IVPLCRNLGIGIVPYSPLGRGFFA-GKAVAESLPAESLLSLHPRFIKENLEKNKCFYTRI 199
           I+P CR LGIGIV YSPLGRGFFA G  + E+L    +    PRF +ENL+ NK  + ++
Sbjct: 194 IIPTCRELGIGIVAYSPLGRGFFASGPKLVENLDNNDVRKTLPRFQQENLDHNKILFEKV 253

Query: 200 EKLAEKPHCSPAQLSLAFL-QQGDDVVPIPSTTKIKNLEQNIGSLTVKLDKDNLNEISEA 259
             ++EK  C+PAQL+LA++  QGDDV PIP TTKI+NL QNIG+L+VKL  + ++E+   
Sbjct: 254 SAMSEKKGCTPAQLALAWVHHQGDDVCPIPGTTKIENLNQNIGALSVKLTPEEMSELESL 313

Query: 260 VPESEVAGNRSYDSMMHATWKYANSPPL 286
                V G RS   +   T+K + +PPL
Sbjct: 314 AQPGFVKGERSISIL--TTFKNSETPPL 339

BLAST of Csor.00g270830 vs. TAIR 10
Match: AT1G60680.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 297.4 bits (760), Expect = 1.3e-80
Identity = 155/269 (57.62%), Postives = 196/269 (72.86%), Query Frame = 0

Query: 20  EALKQLPREKVQIATKFGITWV-GHSMTVKGTPGYVRSCCEASLKRLDIDYIDLYYQHRS 79
           +ALK   +EKV++ATKFG   V G    V+G P YVR+ CEASLKRLDI  IDLYYQHR 
Sbjct: 74  KALKDGVKEKVELATKFGFFIVEGEISEVRGDPEYVRAACEASLKRLDIACIDLYYQHRI 133

Query: 80  DTSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHHITALQMEWSIWARDIED 139
           DT  PIE TM ELKKLV+EGKIKYIGLSEA+  TIRRAHAVH ITA+Q+EWS+W+RD E+
Sbjct: 134 DTRVPIEITMRELKKLVEEGKIKYIGLSEASASTIRRAHAVHPITAVQIEWSLWSRDAEE 193

Query: 140 EIVPLCRNLGIGIVPYSPLGRGFF-AGKAVAESLPAESLLSLHPRFIKENLEKNKCFYTR 199
           +I+P+CR LGIGIV YSPLGRGF  AG  +AE+L  +      PRF +EN++ NK  + +
Sbjct: 194 DIIPICRELGIGIVAYSPLGRGFLAAGPKLAENLENDDFRKTLPRFQQENVDHNKILFEK 253

Query: 200 IEKLAEKPHCSPAQLSLAFL-QQGDDVVPIPSTTKIKNLEQNIGSLTVKLDKDNLNEISE 259
           +  +AEK  C+PAQL+LA++  QGDDV PIP TTKI+NL QNI +L+VKL  + ++E+  
Sbjct: 254 VSAMAEKKGCTPAQLALAWVHHQGDDVCPIPGTTKIENLNQNIRALSVKLTPEEISELDS 313

Query: 260 AVPESEVAGNRSYDSMMHATWKYANSPPL 286
                 V G R   SM  +T+K +N+PPL
Sbjct: 314 LAKPESVKGERYMASM--STFKNSNTPPL 340

BLAST of Csor.00g270830 vs. TAIR 10
Match: AT1G10810.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 289.7 bits (740), Expect = 2.7e-78
Identity = 148/268 (55.22%), Postives = 191/268 (71.27%), Query Frame = 0

Query: 20  EALKQLPREKVQIATKFGITWVGHSMTVKGTPGYVRSCCEASLKRLDIDYIDLYYQHRSD 79
           +ALK   REKV++ATKFG+      +  +G P YVR+ CEASL+RL +  IDLYYQHR D
Sbjct: 74  QALKDGMREKVELATKFGLLLKDQKLGYRGDPAYVRAACEASLRRLGVSCIDLYYQHRID 133

Query: 80  TSTPIEETMGELKKLVQEGKIKYIGLSEANPQTIRRAHAVHHITALQMEWSIWARDIEDE 139
           T+ PIE T+GELKKLV+EGKIKYIGLSEA   TIRRAHAVH +TA+Q+EWS+W+RD+E++
Sbjct: 134 TTVPIEVTIGELKKLVEEGKIKYIGLSEACASTIRRAHAVHPLTAVQLEWSLWSRDVEED 193

Query: 140 IVPLCRNLGIGIVPYSPLGRGFF-AGKAVAESLPAESLLSLHPRFIKENLEKNKCFYTRI 199
           I+P CR LGIGIV YSPLG GFF AG    ES+         PRF +ENL+ NK  Y ++
Sbjct: 194 IIPTCRELGIGIVAYSPLGLGFFAAGPKFIESMDNGDYRKGLPRFQQENLDHNKILYEKV 253

Query: 200 EKLAEKPHCSPAQLSLAFL-QQGDDVVPIPSTTKIKNLEQNIGSLTVKLDKDNLNEISEA 259
             +AEK  C+PAQL+LA++  QG+DV PIP T+KIKNL QNIG+L+VKL  + + E+   
Sbjct: 254 NAMAEKKSCTPAQLALAWVHHQGNDVCPIPGTSKIKNLNQNIGALSVKLSIEEMAELDAM 313

Query: 260 VPESEVAGNRSYDSMMHATWKYANSPPL 286
                V G RS   ++  T+K + +PPL
Sbjct: 314 GHPDSVKGERSATYIV--TYKNSETPPL 339

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C6TBN21.1e-10570.04Probable aldo-keto reductase 1 OS=Glycine max OX=3847 GN=AKR1 PE=2 SV=1[more]
Q3L1812.1e-10469.03Perakine reductase OS=Rauvolfia serpentina OX=4060 GN=PR PE=1 SV=1[more]
Q93ZN23.9e-8258.96Probable aldo-keto reductase 4 OS=Arabidopsis thaliana OX=3702 GN=At1g60710 PE=2... [more]
O227071.9e-8156.62Probable aldo-keto reductase 3 OS=Arabidopsis thaliana OX=3702 GN=At1g60690 PE=3... [more]
Q9ASZ92.1e-8056.34Probable aldo-keto reductase 5 OS=Arabidopsis thaliana OX=3702 GN=At1g60730 PE=2... [more]
Match NameE-valueIdentityDescription
KAG6586306.18.12e-211100.00putative aldo-keto reductase 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023537103.13.92e-18679.42perakine reductase-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023537102.11.07e-18379.13probable aldo-keto reductase 1 [Cucurbita pepo subsp. pepo][more]
XP_022937674.15.08e-18278.26perakine reductase-like isoform X1 [Cucurbita moschata] >XP_022937675.1 perakine... [more]
XP_022965820.11.02e-18177.39probable aldo-keto reductase 1 isoform X1 [Cucurbita maxima] >XP_022965821.1 pro... [more]
Match NameE-valueIdentityDescription
A0A6J1FBV82.46e-18278.26perakine reductase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444009... [more]
A0A6J1HPU04.96e-18277.39probable aldo-keto reductase 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1HQ362.43e-18092.59probable aldo-keto reductase 1 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1HS011.11e-17876.52probable aldo-keto reductase 1 OS=Cucurbita maxima OX=3661 GN=LOC111465592 PE=4 ... [more]
A0A6J1FB171.64e-17892.59probable aldo-keto reductase 1 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT1G60710.12.8e-8358.96NAD(P)-linked oxidoreductase superfamily protein [more]
AT1G60690.11.4e-8256.62NAD(P)-linked oxidoreductase superfamily protein [more]
AT1G60730.11.5e-8156.34NAD(P)-linked oxidoreductase superfamily protein [more]
AT1G60680.11.3e-8057.62NAD(P)-linked oxidoreductase superfamily protein [more]
AT1G10810.12.7e-7855.22NAD(P)-linked oxidoreductase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 232..252
NoneNo IPR availablePANTHERPTHR43625AFLATOXIN B1 ALDEHYDE REDUCTASEcoord: 21..284
NoneNo IPR availablePANTHERPTHR43625:SF62ALDO-KETO REDUCTASE 1-RELATEDcoord: 21..284
IPR023210NADP-dependent oxidoreductase domainPFAMPF00248Aldo_ket_redcoord: 20..257
e-value: 7.2E-54
score: 182.9
IPR036812NADP-dependent oxidoreductase domain superfamilyGENE3D3.20.20.100coord: 18..286
e-value: 7.1E-86
score: 290.1
IPR036812NADP-dependent oxidoreductase domain superfamilySUPERFAMILY51430NAD(P)-linked oxidoreductasecoord: 20..259

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g270830.m01Csor.00g270830.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0047834 D-threo-aldose 1-dehydrogenase activity