Csor.00g270490 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g270490
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionReticulon-like protein
LocationCsor_Chr12: 15140288 .. 15144001 (+)
RNA-Seq ExpressionCsor.00g270490
SyntenyCsor.00g270490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTTGAGCCGGCGGAGAGCGGCGGTGGCAAGGGGGAACGTGGTGGCGGGGTCTGTCTGGGAAAGCAGAATCCGATTCGATGAAGTCCGTGGTGGGATTAGGGTTTTCAATGGAGACGATGAAACCGCCGAATGTAGGGTTCCGGCCGCTACCGTCGGGAGTGCAAGGAGGAAGACGTGGAAATCCGATGAGGGTTTTAACTCGATTCAAATCGCCGAGGAAAAACCAGAATCACCGAGTAGCGGCGATGAACAGAATCGGAAAACCCCGATTCCGTCTCGGAGATTGAGATCCAACAACAGTCCAAACAAACCGGTACGAATAACAGAGAGAAACTCAACGAGGAAGAAAACCGATCAGTCTCGAAAATCGGCCGTCGGGATTATCAAACCGCCGACGAACACAAACGGAATCAGAAAAATCTCCCCGGAGAAGACGGTTTCATCTCCTCCACCTCAAGATTTCTTCGATGCATTCGAAGAGATTAAGAAAGAAAGCTTCGACGTTAAGGAAATCAATTTTCCAGCCCAAGAAATTTTCCCGGGAAACAAACAGAAATTACAGAGACTAAGTGAGAACAGAGCCTCCCATTTTCATTACAGAATCTCAAAATTTAATTTCCTTAACTGTTTGATTTCTTGCAGTGGATTTGATAATGTGGAGGGAGTTTTCAAGATCCGCCATGGCATTTGGAGCTGGGAATTTGGTTATAGCCCTCTCTTATTTCATCAAGAACATGAACATCAGGTAAAAACAAAAAAAAAAAAAAAAAAAAACCCCAAATCCGCCATTGAAGAAGGGTTGTTGAGCTTTGTTTTGTTTTTCGAATTTGCAGCTTTATCAGTATGATTTCGCATATGGGTCTTGTTTATCTGGCTGCCATTTTCGTCCATAGCTCCATTTTTAGCAGGTGCTCGATCAATTTCGTTCTGTTTTGTTTAGCTTAAGCTTGTTCCTAGAAAAGAATACTCTCTACGAGACTTCTTGGGAAAGCCCAGGGAAAATTACAGGGGAAATGGAAAGGGAATCGATAGTGATGATGAACATTGTGTGGTTGAAGAAGAAGTGATTCGATTGGTTAAAGGGTTTGTTCCTTTGGTGAATGAATTGCTGCAAAATCTCAAGCCTCTGTTCCATGGAGATCCTTCTGCAACTATGAAGGTAATTTTCTTTTCTTTTTTCTTTTTTATTTTTAAAATTAAAAAATATATATACTCTTAAATTTTCTATTTTCTTTACTCAATTACACGTTTTTTCCAACTAAAATTATTTTATTTAAAAAATATTTTTAAATTTTAATAGTATTTTAAATATATTATTGAATTTAAAATATTTCATTAATATCTTTCCATTTTTATTTTTTAAAAAGTTTTTAAAAAAATATACATAAAATTTTATAAATAATATTAACACTATGAACAAAAACGGTCTATCTACACTATATAGATTATTTTCGAACTTTTTAATGTTATTTTTGAAAAATCACGAATAATTTTGAAACGGGTTACTAACGTTAAAAATATTTTTAAATATTTTTAAAAATTGAAGCTTATTATTAATGAAAATTCATGCATATTTTTTCAGCGTGTTACTAGGTTAAAAATATTTATTTTTTTTTTTAAAAAATTGAATTTATTATTAATGAAAATTCATGCATAATTTTGAAACGGGTTACTAATGTTAAAAATATTTTTAATTTTTTTAAAAAATTGAAGCTTATTATTAATGAAAATTTTGAAAATTCAACCGTATTTTAAAAATATTTGTAAAAATTAAACGGCATTTTAAAAATATTTTTATAAGTGTAAGAAAATTTTAAAATATAGTATTCACCATCTTCGCCCAAAATTAATGTTAATGATATTTTAAAACTTTTTTGAAAATTTAAGAATATTTTTATACGAGATAAAAGTAAAATTTATTGTTATCTTTTACCATTTTTGAAGAGGGTTAAAAATGGGCAATTATGGTAAGAAATATAGAAATATATGGTAAAAAGAAAGTGTAATAAATATTTATTAAAATTTAAAATTGTTTGGTAATTTGCAGATGGGCGTACTGCTGTTCGTGTTGGCAAAGTGGGGCAGCTTCATCACCTTTTGGAATATCCTCCAATGCGGTAAATTTTTCTTTTACCATCTTTTTTTCATCCACCTAATTTCACTTTCTTTTTTTAAAATAAAAATAAGCTTTTAAATATTGAAAGAAATAATTTTGTGTTTTTTTTTTTTTTTTTTTTTTTGGGTTCTGACAAATGGATGTACTGATTGACTGGCACAGGATTCGTTGCGGTTTTCACCCTCCCCAAATTATTTACCCACGGTAAAAAAACACATCTCCACGGACTTAACTTTTCTATCTTTTATTCTTTTTTGGGTTACAATTCAATACGGCCGAGTACAACAAACCACCGTACACCGCTGTCTTTTCATCCGTCGATTTGTTTGTGACCCTCTTTTGTTCCCTTCATAAAACGACCACGTTTTAAATTCTAAAATTTCGAAAAGGGAATAAAAATTAAAATTGTTATATACTTTATTTGTCGGTAATTTATTTTCTTCTCTGTAAGTTCTCGTGTCAGTTCTGTCGGATCGGATGATTTGATGTTTGATTCAATCTCAACCCTTCATTTTTCTTTTCTTTTTTTGTGTTTTAATTTTAAAATTAAAAATAAATATTTTATTTTCATCTTTGGATTCTAAAAACTTCAATTTGGTTCGCGACTTATTTTTGTTTTTATGTTTTCATTTGAATCACATGAAACTTTAGCTTTAAATATCTAATTATTCTCTTAGATAGCGTCTACTCCATTCTTAAATTTTGAAACGGGTTTAGATCCTAAAAGGTGTCTTATTCATCGAACTATCCTTAGGTTAGTAGAAACATAATGACTTCCTAATACGAGGGAGCTACGTGTCTTTCAAGTTGCCACACGAAAATGGTTTCAGTCTCGGCCAATTTTCGTTCGACCGATGTCGGGTACCATTAGACAGTTCTGAAAAGTTGGTTTGTTTTTTCAATATCTTAATATTCCCTTGGGAAACCGTGTTAGGACTCGAATTTTGAAGAGAAAAGAGCTTATAGTACTATGCTTACGAGCTTGAAATCGATACCTTAATTGGGTTGTTTGGAGCACACATGCGGAAAAATTGATTATTAGTCTTTGCTATATGAACGCCCATTTGCCCTTTTGATCGGGACGATCGAGGTCTTTGTTTATCGACTTTTTGTTTGTCTATGTGTGTTTCTTTACTTTACTCGCGTGGGAGAAAGGAAGAAACAAAAATGAAGAAACTAAATCGTTATCAAACGAAGTTTATTTAGAATATGACGCGGATTCGTGTTGATGTTTGTAAGAGTTCTTTTTTTTTTTGTGAATTAGGAGAACATTGGTTGAGGCTTTGTGGGAGTTCTTGGCAGCTGAGTTCCCACAAGAAGGGAATGATTGTTGCTATCTTCCTTATGGTATGGAACTTCTCTTCGATTCTATATCGCGTTTGGGCAGGTAAACCATTTCGAACTGAAATCTTAATCTGTTTCGAGATGTAGATTCGAACGTATAACGTTATTGAAATGAAGTATAATTTTGCAGCTTTCATAGCGTTTGTGAGCTTTCGCTACTACCGGGAATCATTAGGGAGAGATGGTTGGGTTAAAAATGGAGAAGCAGCTCGACATAACGACACGGTTTCGGGTAAGTTCAAAGCTTGA

mRNA sequence

ATGGATTTGAGCCGGCGGAGAGCGGCGGTGGCAAGGGGGAACGTGGTGGCGGGGTCTGTCTGGGAAAGCAGAATCCGATTCGATGAAGTCCGTGGTGGGATTAGGGTTTTCAATGGAGACGATGAAACCGCCGAATGTAGGGTTCCGGCCGCTACCGTCGGGAGTGCAAGGAGGAAGACGTGGAAATCCGATGAGGGTTTTAACTCGATTCAAATCGCCGAGGAAAAACCAGAATCACCGAGTAGCGGCGATGAACAGAATCGGAAAACCCCGATTCCGTCTCGGAGATTGAGATCCAACAACAGTCCAAACAAACCGGTACGAATAACAGAGAGAAACTCAACGAGGAAGAAAACCGATCAGTCTCGAAAATCGGCCGTCGGGATTATCAAACCGCCGACGAACACAAACGGAATCAGAAAAATCTCCCCGGAGAAGACGGTTTCATCTCCTCCACCTCAAGATTTCTTCGATGCATTCGAAGAGATTAAGAAAGAAAGCTTCGACGTTAAGGAAATCAATTTTCCAGCCCAAGAAATTTTCCCGGGAAACAAACAGAAATTACAGAGACTAATGGATTTGATAATGTGGAGGGAGTTTTCAAGATCCGCCATGGCATTTGGAGCTGGGAATTTGGTTATAGCCCTCTCTTATTTCATCAAGAACATGAACATCAGCTTTATCAGTATGATTTCGCATATGGGTCTTGTTTATCTGGCTGCCATTTTCGTCCATAGCTCCATTTTTAGCAGGGGAAATGGAAAGGGAATCGATAGTGATGATGAACATTGTGTGGTTGAAGAAGAAGTGATTCGATTGGTTAAAGGGTTTGTTCCTTTGGTGAATGAATTGCTGCAAAATCTCAAGCCTCTGTTCCATGGAGATCCTTCTGCAACTATGAAGATGGGCGTACTGCTGTTCGTGTTGGCAAAGTGGGGCAGCTTCATCACCTTTTGGAATATCCTCCAATGCGGATTCGTTGCGGTTTTCACCCTCCCCAAATTATTTACCCACGGAGAACATTGGTTGAGGCTTTGTGGGAGTTCTTGGCAGCTGAGTTCCCACAAGAAGGGAATGATTGTTGCTATCTTCCTTATGGTATGGAACTTCTCTTCGATTCTATATCGCGTTTGGGCAGCTTTCATAGCGTTTGTGAGCTTTCGCTACTACCGGGAATCATTAGGGAGAGATGGTTGGGTTAAAAATGGAGAAGCAGCTCGACATAACGACACGGTTTCGGGTAAGTTCAAAGCTTGA

Coding sequence (CDS)

ATGGATTTGAGCCGGCGGAGAGCGGCGGTGGCAAGGGGGAACGTGGTGGCGGGGTCTGTCTGGGAAAGCAGAATCCGATTCGATGAAGTCCGTGGTGGGATTAGGGTTTTCAATGGAGACGATGAAACCGCCGAATGTAGGGTTCCGGCCGCTACCGTCGGGAGTGCAAGGAGGAAGACGTGGAAATCCGATGAGGGTTTTAACTCGATTCAAATCGCCGAGGAAAAACCAGAATCACCGAGTAGCGGCGATGAACAGAATCGGAAAACCCCGATTCCGTCTCGGAGATTGAGATCCAACAACAGTCCAAACAAACCGGTACGAATAACAGAGAGAAACTCAACGAGGAAGAAAACCGATCAGTCTCGAAAATCGGCCGTCGGGATTATCAAACCGCCGACGAACACAAACGGAATCAGAAAAATCTCCCCGGAGAAGACGGTTTCATCTCCTCCACCTCAAGATTTCTTCGATGCATTCGAAGAGATTAAGAAAGAAAGCTTCGACGTTAAGGAAATCAATTTTCCAGCCCAAGAAATTTTCCCGGGAAACAAACAGAAATTACAGAGACTAATGGATTTGATAATGTGGAGGGAGTTTTCAAGATCCGCCATGGCATTTGGAGCTGGGAATTTGGTTATAGCCCTCTCTTATTTCATCAAGAACATGAACATCAGCTTTATCAGTATGATTTCGCATATGGGTCTTGTTTATCTGGCTGCCATTTTCGTCCATAGCTCCATTTTTAGCAGGGGAAATGGAAAGGGAATCGATAGTGATGATGAACATTGTGTGGTTGAAGAAGAAGTGATTCGATTGGTTAAAGGGTTTGTTCCTTTGGTGAATGAATTGCTGCAAAATCTCAAGCCTCTGTTCCATGGAGATCCTTCTGCAACTATGAAGATGGGCGTACTGCTGTTCGTGTTGGCAAAGTGGGGCAGCTTCATCACCTTTTGGAATATCCTCCAATGCGGATTCGTTGCGGTTTTCACCCTCCCCAAATTATTTACCCACGGAGAACATTGGTTGAGGCTTTGTGGGAGTTCTTGGCAGCTGAGTTCCCACAAGAAGGGAATGATTGTTGCTATCTTCCTTATGGTATGGAACTTCTCTTCGATTCTATATCGCGTTTGGGCAGCTTTCATAGCGTTTGTGAGCTTTCGCTACTACCGGGAATCATTAGGGAGAGATGGTTGGGTTAAAAATGGAGAAGCAGCTCGACATAACGACACGGTTTCGGGTAAGTTCAAAGCTTGA

Protein sequence

MDLSRRRAAVARGNVVAGSVWESRIRFDEVRGGIRVFNGDDETAECRVPAATVGSARRKTWKSDEGFNSIQIAEEKPESPSSGDEQNRKTPIPSRRLRSNNSPNKPVRITERNSTRKKTDQSRKSAVGIIKPPTNTNGIRKISPEKTVSSPPPQDFFDAFEEIKKESFDVKEINFPAQEIFPGNKQKLQRLMDLIMWREFSRSAMAFGAGNLVIALSYFIKNMNISFISMISHMGLVYLAAIFVHSSIFSRGNGKGIDSDDEHCVVEEEVIRLVKGFVPLVNELLQNLKPLFHGDPSATMKMGVLLFVLAKWGSFITFWNILQCGFVAVFTLPKLFTHGEHWLRLCGSSWQLSSHKKGMIVAIFLMVWNFSSILYRVWAAFIAFVSFRYYRESLGRDGWVKNGEAARHNDTVSGKFKA
Homology
BLAST of Csor.00g270490 vs. ExPASy Swiss-Prot
Match: Q56X72 (Reticulon-like protein B21 OS=Arabidopsis thaliana OX=3702 GN=RTNLB21 PE=2 SV=2)

HSP 1 Score: 188.3 bits (477), Expect = 1.7e-46
Identity = 122/352 (34.66%), Postives = 195/352 (55.40%), Query Frame = 0

Query: 59  KTWKSDEGFNSIQIAEEKPESPSSGDEQNRKTPIPSRRLRSNNSPNKPVRITERNSTRKK 118
           K+ ++++  N  Q   ++ +    GDE+        R +   +   K + + + N  R  
Sbjct: 113 KSNENEQIDNGDQEIGDQDDYEEDGDEEE------EREVEKKSVDVKEINVAKEN--RVG 172

Query: 119 TDQSRKSAVGIIKPPTNTNGIRKISP-----EKTVSSPPPQDFFDAFEEIKKESFDVKEI 178
             + +K +    +   + + +RKISP       +V S PP            + F  +E 
Sbjct: 173 GVEIKKFSQFQNRTSPSPSSVRKISPPVIKRATSVYSAPPN------STSSTDRFAEQED 232

Query: 179 NFPAQEIFPGNKQKLQRLMDLIMWREFSRSAMAFGAGNLVIALSYFIKNMNISFISMISH 238
           N      F  ++ KLQ L+DL+MWR+ SRS + FG G  +I  S +  ++N SFIS++++
Sbjct: 233 N------FTHSQSKLQSLVDLVMWRDVSRSTLVFGFGTFLIISSSYANDLNFSFISVVAY 292

Query: 239 MGLVYLAAIFVHSSIFSRGNGKGIDSDDEHCVV---EEEVIRLVKGFVPLVNELLQNLKP 298
           MGL+YL  +FV  S+  R    G+  ++ H VV   EE+V R+++  +P +NE L  L+ 
Sbjct: 293 MGLIYLGLMFVLKSLIHR----GMVEEERHKVVGVREEDVKRMLRLIMPYLNESLHQLRA 352

Query: 299 LFHGDPSATMKMGVLLFVLAKWGSFITFWNILQCGFVAVFTLPKLF--------THGEHW 358
           LF GDPS T+KMGV+LFVLA+ GS IT WN+ + GF+  FT+PK+F         +G  W
Sbjct: 353 LFSGDPSTTLKMGVVLFVLARCGSSITLWNLAKFGFLGAFTIPKIFISYSTHFSAYGNFW 412

Query: 359 LRLCGSSWQLSSHKKGMIVAIFLMVWNFSSILYRVWAAFIAFVSFRYYRESL 395
           +R    +W+  +HKK + +A+F +VWN SS+  RVWAAF+  V+FRYY+  +
Sbjct: 413 MRRFRDAWESCNHKKAVALALFTLVWNLSSVTARVWAAFMLLVAFRYYQHKM 440

BLAST of Csor.00g270490 vs. ExPASy Swiss-Prot
Match: Q6DR04 (Reticulon-like protein B17 OS=Arabidopsis thaliana OX=3702 GN=RTNLB17 PE=2 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 5.2e-27
Identity = 72/212 (33.96%), Postives = 113/212 (53.30%), Query Frame = 0

Query: 191 LMDLIMWREFSRSAMAFGAGNLVIALSYFIKNMNISFISMISHMGLVYLAAIFVHSSIFS 250
           + DL+MWR+ ++S + FG G L    S F K +N S  S +S++GLV L   F+ +++  
Sbjct: 168 ISDLVMWRDVAKSTLWFGFGCLSFLSSCFAKGVNFSVFSAVSNLGLVLLCGSFLSNTLCQ 227

Query: 251 RGNGKGIDSDDEHCVVEEEVIRLVKGFVPLVNELLQNLKPLFHGDPSATMKMGVLLFVLA 310
           R   K  D+     V E++V+R  +  +P  N  +     LF G+PS T+K+   L + A
Sbjct: 228 R---KNEDTKRAFHVSEDDVLRSARRVLPATNFFISKTSELFSGEPSMTLKVTPFLLLGA 287

Query: 311 KWGSFITFWNILQCGFVAVFTLPKLFTHGEHWL--------RLCGSSWQLSSHKKGMIVA 370
           ++G  IT W +   GF   FT+PKL++   H +           G +W + SHKK +  +
Sbjct: 288 EYGHLITLWRLSAFGFFLSFTIPKLYSCYTHQISQKVERVKTRIGEAWGVCSHKKILAGS 347

Query: 371 IFLMVWNFSSILYRVWAAFIAFVSFRYYRESL 395
                WN +SI  R++A FI  V FRY R++L
Sbjct: 348 AVTAFWNLTSIRTRIFAVFIILVIFRYRRQNL 376

BLAST of Csor.00g270490 vs. ExPASy Swiss-Prot
Match: Q8LDS3 (Reticulon-like protein B18 OS=Arabidopsis thaliana OX=3702 GN=RTNLB18 PE=2 SV=2)

HSP 1 Score: 95.1 bits (235), Expect = 2.0e-18
Identity = 91/358 (25.42%), Postives = 166/358 (46.37%), Query Frame = 0

Query: 72  IAEEKPESPSSGDEQNRKTPIPSRRLRSNNSPNKPVRITERNST--RKKTD--------- 131
           ++   P SP +  E    +P P R+ R+  S    +   E   T  R KT          
Sbjct: 54  VSLSSPSSPVTLREILLLSPSPLRKSRTRLSNRFDMEAAEAAVTARRSKTKGGQNGLLAS 113

Query: 132 -------QSRKSAVGIIKPPTNTNGIRKISPEKTVSSPPPQDFFDAFEEIKKESFDVKEI 191
                  +SR  +  ++    NT  I  ++ EK  +    +    + +E K  S  +   
Sbjct: 114 PSPRNFRRSRLRSEAMVDTKENTEPIVVVTDEKKQNQRKQKKLGRSKKE-KHSSVPLLAS 173

Query: 192 NFPA----QEIFPGNKQKL-QRLMDLIMWREFSRSAMAFGAGNL-VIALSYFIKNMNISF 251
             P+    Q++  G+ +++ + + DLIMWR+ ++S + FG G +  ++  +  K  N S 
Sbjct: 174 PSPSSDQPQDVCQGDLERIRENISDLIMWRDVAKSTLWFGFGCICFLSTCFAAKGFNFSV 233

Query: 252 ISMISHMGLVYLAAIFVHSSIFSRGNGKGIDSDDEHCVVEEEVIRLVKGFVPLVNELLQN 311
            S IS++GL++L   F+ +++  R      ++  E  + E++V+R+ +  +P+ N  +  
Sbjct: 234 FSAISYLGLLFLGVSFLSNTLRQRVTE---EARRELKLSEDDVLRIARRMLPITNLAISK 293

Query: 312 LKPLFHGDPSATMKMGVLLFVLAKWGSFITFWNILQCGFVAVFTLPKLFTHGEHWL---- 371
              LF G+P+ T+K+   + + A++G  IT W +   GF   FT+PKL++     L    
Sbjct: 294 TSELFSGEPAMTLKVAPFVLMGAEYGYLITLWRLCAFGFFLSFTIPKLYSCYASQLNQKV 353

Query: 372 ----RLCGSSWQLSSHKKGMIVAIFLMVWNFSSILYRVWAAFIAFVSFRYYRESLGRD 398
               R    +W + +HKK +  +     WN +S+  R  A FI  V  RY R++L  D
Sbjct: 354 ECAQRRFVEAWGVCTHKKFVAGSAVTAFWNLTSLKTRFIAVFIIVVVIRYRRQNLQLD 407

BLAST of Csor.00g270490 vs. NCBI nr
Match: KAG6586339.1 (Reticulon-like protein B21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 824 bits (2128), Expect = 5.90e-301
Identity = 418/418 (100.00%), Postives = 418/418 (100.00%), Query Frame = 0

Query: 1   MDLSRRRAAVARGNVVAGSVWESRIRFDEVRGGIRVFNGDDETAECRVPAATVGSARRKT 60
           MDLSRRRAAVARGNVVAGSVWESRIRFDEVRGGIRVFNGDDETAECRVPAATVGSARRKT
Sbjct: 1   MDLSRRRAAVARGNVVAGSVWESRIRFDEVRGGIRVFNGDDETAECRVPAATVGSARRKT 60

Query: 61  WKSDEGFNSIQIAEEKPESPSSGDEQNRKTPIPSRRLRSNNSPNKPVRITERNSTRKKTD 120
           WKSDEGFNSIQIAEEKPESPSSGDEQNRKTPIPSRRLRSNNSPNKPVRITERNSTRKKTD
Sbjct: 61  WKSDEGFNSIQIAEEKPESPSSGDEQNRKTPIPSRRLRSNNSPNKPVRITERNSTRKKTD 120

Query: 121 QSRKSAVGIIKPPTNTNGIRKISPEKTVSSPPPQDFFDAFEEIKKESFDVKEINFPAQEI 180
           QSRKSAVGIIKPPTNTNGIRKISPEKTVSSPPPQDFFDAFEEIKKESFDVKEINFPAQEI
Sbjct: 121 QSRKSAVGIIKPPTNTNGIRKISPEKTVSSPPPQDFFDAFEEIKKESFDVKEINFPAQEI 180

Query: 181 FPGNKQKLQRLMDLIMWREFSRSAMAFGAGNLVIALSYFIKNMNISFISMISHMGLVYLA 240
           FPGNKQKLQRLMDLIMWREFSRSAMAFGAGNLVIALSYFIKNMNISFISMISHMGLVYLA
Sbjct: 181 FPGNKQKLQRLMDLIMWREFSRSAMAFGAGNLVIALSYFIKNMNISFISMISHMGLVYLA 240

Query: 241 AIFVHSSIFSRGNGKGIDSDDEHCVVEEEVIRLVKGFVPLVNELLQNLKPLFHGDPSATM 300
           AIFVHSSIFSRGNGKGIDSDDEHCVVEEEVIRLVKGFVPLVNELLQNLKPLFHGDPSATM
Sbjct: 241 AIFVHSSIFSRGNGKGIDSDDEHCVVEEEVIRLVKGFVPLVNELLQNLKPLFHGDPSATM 300

Query: 301 KMGVLLFVLAKWGSFITFWNILQCGFVAVFTLPKLFTHGEHWLRLCGSSWQLSSHKKGMI 360
           KMGVLLFVLAKWGSFITFWNILQCGFVAVFTLPKLFTHGEHWLRLCGSSWQLSSHKKGMI
Sbjct: 301 KMGVLLFVLAKWGSFITFWNILQCGFVAVFTLPKLFTHGEHWLRLCGSSWQLSSHKKGMI 360

Query: 361 VAIFLMVWNFSSILYRVWAAFIAFVSFRYYRESLGRDGWVKNGEAARHNDTVSGKFKA 418
           VAIFLMVWNFSSILYRVWAAFIAFVSFRYYRESLGRDGWVKNGEAARHNDTVSGKFKA
Sbjct: 361 VAIFLMVWNFSSILYRVWAAFIAFVSFRYYRESLGRDGWVKNGEAARHNDTVSGKFKA 418

BLAST of Csor.00g270490 vs. NCBI nr
Match: XP_022938288.1 (reticulon-like protein B21 [Cucurbita moschata])

HSP 1 Score: 811 bits (2095), Expect = 6.32e-296
Identity = 410/418 (98.09%), Postives = 413/418 (98.80%), Query Frame = 0

Query: 1   MDLSRRRAAVARGNVVAGSVWESRIRFDEVRGGIRVFNGDDETAECRVPAATVGSARRKT 60
           MDLSRRRAAVARGNVVAGSVWESRIRFDEVRGGIRVFNGDDET ECRVPAATVGSARRKT
Sbjct: 1   MDLSRRRAAVARGNVVAGSVWESRIRFDEVRGGIRVFNGDDETVECRVPAATVGSARRKT 60

Query: 61  WKSDEGFNSIQIAEEKPESPSSGDEQNRKTPIPSRRLRSNNSPNKPVRITERNSTRKKTD 120
           WKSDEGFNSIQIAEEKPESPSSGDEQNRKTPIPSRRLRSNNSPNKPVRITERNSTRKK D
Sbjct: 61  WKSDEGFNSIQIAEEKPESPSSGDEQNRKTPIPSRRLRSNNSPNKPVRITERNSTRKKID 120

Query: 121 QSRKSAVGIIKPPTNTNGIRKISPEKTVSSPPPQDFFDAFEEIKKESFDVKEINFPAQEI 180
           QSRKSAVGI KPPTNTNGIRKISP+KTVSSPPPQDFFDAFEEIK ESFDVKEINFPAQEI
Sbjct: 121 QSRKSAVGITKPPTNTNGIRKISPDKTVSSPPPQDFFDAFEEIKNESFDVKEINFPAQEI 180

Query: 181 FPGNKQKLQRLMDLIMWREFSRSAMAFGAGNLVIALSYFIKNMNISFISMISHMGLVYLA 240
           FPGNKQKLQRLMDLIMWREFSRSAMAFGAGNLVIALSYFIKNMNISFISMISHMGLVYLA
Sbjct: 181 FPGNKQKLQRLMDLIMWREFSRSAMAFGAGNLVIALSYFIKNMNISFISMISHMGLVYLA 240

Query: 241 AIFVHSSIFSRGNGKGIDSDDEHCVVEEEVIRLVKGFVPLVNELLQNLKPLFHGDPSATM 300
           AIFVHSSIFSRGNGKGIDSDDEHCVVEEEVIRLV+GFVP VNELLQNLKPLFHGDPSAT+
Sbjct: 241 AIFVHSSIFSRGNGKGIDSDDEHCVVEEEVIRLVRGFVPFVNELLQNLKPLFHGDPSATL 300

Query: 301 KMGVLLFVLAKWGSFITFWNILQCGFVAVFTLPKLFTHGEHWLRLCGSSWQLSSHKKGMI 360
           KMGVLLFVLAKWGSFITFWNILQCGFVAVFTLPKLFTHGEHWLRLCGSSWQLSSHKKGMI
Sbjct: 301 KMGVLLFVLAKWGSFITFWNILQCGFVAVFTLPKLFTHGEHWLRLCGSSWQLSSHKKGMI 360

Query: 361 VAIFLMVWNFSSILYRVWAAFIAFVSFRYYRESLGRDGWVKNGEAARHNDTVSGKFKA 418
           VAIFLMVWNFSSILYRVWAAFIAFVSFRYYRESLGRDGWVKNGEAARHNDTVSGKFKA
Sbjct: 361 VAIFLMVWNFSSILYRVWAAFIAFVSFRYYRESLGRDGWVKNGEAARHNDTVSGKFKA 418

BLAST of Csor.00g270490 vs. NCBI nr
Match: XP_022965768.1 (reticulon-like protein B21 [Cucurbita maxima])

HSP 1 Score: 800 bits (2065), Expect = 2.36e-291
Identity = 405/417 (97.12%), Postives = 408/417 (97.84%), Query Frame = 0

Query: 1   MDLSRRRAAVARGNVVAGSVWESRIRFDEVRGGIRVFNGDDETAECRVPAATVGSARRKT 60
           MDLSRRRAAVARGNVVAGSVWESRIRFDEVRGGIRVFNGDDETAECRVPAATVGSARRKT
Sbjct: 1   MDLSRRRAAVARGNVVAGSVWESRIRFDEVRGGIRVFNGDDETAECRVPAATVGSARRKT 60

Query: 61  WKSDEGFNSIQIAEEKPESPSSGDEQNRKTPIPSRRLRSNNSPNKPVRITERNSTRKKTD 120
           WKSDE FNSIQIAEEKPESPSSGDE NRKTPIPSRRLRSNNSPNKPVRITERNSTRKKTD
Sbjct: 61  WKSDESFNSIQIAEEKPESPSSGDEPNRKTPIPSRRLRSNNSPNKPVRITERNSTRKKTD 120

Query: 121 QSRKSAVGIIKPPTNTNGIRKISPEKTVSSPPPQDFFDAFEEIKKESFDVKEINFPAQEI 180
           Q RKSAVGI KPP NTNGIRKISPEKTVSSPPPQDFFDAFEEIK ESFDVKEINFP QEI
Sbjct: 121 QPRKSAVGITKPPMNTNGIRKISPEKTVSSPPPQDFFDAFEEIKNESFDVKEINFPGQEI 180

Query: 181 FPGNKQKLQRLMDLIMWREFSRSAMAFGAGNLVIALSYFIKNMNISFISMISHMGLVYLA 240
           FPGNKQKLQRLMDLIMWREFSRSAMAFGAGNLVIALSYFIKNMNISFISMISHMGLVYLA
Sbjct: 181 FPGNKQKLQRLMDLIMWREFSRSAMAFGAGNLVIALSYFIKNMNISFISMISHMGLVYLA 240

Query: 241 AIFVHSSIFSRGNGKGIDSDDEHCVVEEEVIRLVKGFVPLVNELLQNLKPLFHGDPSATM 300
           AIFVHSSIFSRGNGKGIDSDDEHCVVEEEVIRLVKGFVP VNELLQNLKPLFHGDPSATM
Sbjct: 241 AIFVHSSIFSRGNGKGIDSDDEHCVVEEEVIRLVKGFVPFVNELLQNLKPLFHGDPSATM 300

Query: 301 KMGVLLFVLAKWGSFITFWNILQCGFVAVFTLPKLFTHGEHWLRLCGSSWQLSSHKKGMI 360
           KMG+LLFVLAKWGSFITFWNILQCGFVAVFTLPKLFTHGEHWLRLC SSWQLSSHKKGMI
Sbjct: 301 KMGLLLFVLAKWGSFITFWNILQCGFVAVFTLPKLFTHGEHWLRLCESSWQLSSHKKGMI 360

Query: 361 VAIFLMVWNFSSILYRVWAAFIAFVSFRYYRESLGRDGWVKNGEAARHNDTVSGKFK 417
           VAIFLMVWNFSSILYRVWAAFIAFVSFRYY+ESLGRDGW+KNGEAARHNDTVSGKFK
Sbjct: 361 VAIFLMVWNFSSILYRVWAAFIAFVSFRYYQESLGRDGWLKNGEAARHNDTVSGKFK 417

BLAST of Csor.00g270490 vs. NCBI nr
Match: KAG7021185.1 (Reticulon-like protein B21, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 684 bits (1766), Expect = 1.46e-246
Identity = 361/418 (86.36%), Postives = 362/418 (86.60%), Query Frame = 0

Query: 1   MDLSRRRAAVARGNVVAGSVWESRIRFDEVRGGIRVFNGDDETAECRVPAATVGSARRKT 60
           MDLSRRRA VARGNVVAGSVWESRIRFDEVRGGIRVFNGDDETAECRVPAATVGSARRKT
Sbjct: 1   MDLSRRRAGVARGNVVAGSVWESRIRFDEVRGGIRVFNGDDETAECRVPAATVGSARRKT 60

Query: 61  WKSDEGFNSIQIAEEKPESPSSGDEQNRKTPIPSRRLRSNNSPNKPVRITERNSTRKKTD 120
           WKSDEGFNSIQIAEEKPESPSSGDEQNRKTPIPSRRLRSNNSPNKP    E N +     
Sbjct: 61  WKSDEGFNSIQIAEEKPESPSSGDEQNRKTPIPSRRLRSNNSPNKPKLNEEENRS----- 120

Query: 121 QSRKSAVGIIKPPTNTNGIRKISPEKTVSSPPPQDFFDAFEEIKKESFDVKEINFPAQEI 180
            SRKSAVGIIKPPTNTNGIRKISPEKTVSSPPPQDFFDAFEEIKKESFDVKEINFPAQEI
Sbjct: 121 -SRKSAVGIIKPPTNTNGIRKISPEKTVSSPPPQDFFDAFEEIKKESFDVKEINFPAQEI 180

Query: 181 FPGNKQKLQRLMDLIMWREFSRSAMAFGAGNLVIALSYFIKNMNISFISMISHMGLVYLA 240
           FPGNKQKLQRLMDLIMWREFSRSAMAFGAGNLVIALSYFIKNMNISFISMISHM      
Sbjct: 181 FPGNKQKLQRLMDLIMWREFSRSAMAFGAGNLVIALSYFIKNMNISFISMISHM------ 240

Query: 241 AIFVHSSIFSRGNGKGIDSDDEHCVVEEEVIRLVKGFVPLVNELLQNLKPLFHGDPSATM 300
                                              GFVPLVNELLQNLKPLFHGDPSATM
Sbjct: 241 -----------------------------------GFVPLVNELLQNLKPLFHGDPSATM 300

Query: 301 KMGVLLFVLAKWGSFITFWNILQCGFVAVFTLPKLFTHGEHWLRLCGSSWQLSSHKKGMI 360
           KMGVLLFVLAKWGSFITFWNILQCGFVAVFTLPKLFTHGEHWLRLCGSSWQLSSHKKGMI
Sbjct: 301 KMGVLLFVLAKWGSFITFWNILQCGFVAVFTLPKLFTHGEHWLRLCGSSWQLSSHKKGMI 360

Query: 361 VAIFLMVWNFSSILYRVWAAFIAFVSFRYYRESLGRDGWVKNGEAARHNDTVSGKFKA 418
           VAIFLMVWNFSSILYRVWAAFIAFVSFRYYRESLGR GWVKNGEAARHNDT SGKFKA
Sbjct: 361 VAIFLMVWNFSSILYRVWAAFIAFVSFRYYRESLGRYGWVKNGEAARHNDTASGKFKA 371

BLAST of Csor.00g270490 vs. NCBI nr
Match: XP_038890790.1 (reticulon-like protein B21 [Benincasa hispida] >XP_038890791.1 reticulon-like protein B21 [Benincasa hispida])

HSP 1 Score: 591 bits (1523), Expect = 2.23e-208
Identity = 324/441 (73.47%), Postives = 358/441 (81.18%), Query Frame = 0

Query: 1   MDLSRRRAAVA-RGNVVAGSVWESRIRFDEVRGGIRVFNGDDETAECRVP----AATVGS 60
           MDLSRRRAAVA RGNVVAGSVWESRIRFDEVRGGI+VFNGDD+ AEC +P    AAT GS
Sbjct: 1   MDLSRRRAAVAARGNVVAGSVWESRIRFDEVRGGIKVFNGDDDNAECGIPTTAAAATGGS 60

Query: 61  ARRKTWKSDEGFNSIQIAEEKPES-PSSGDEQNRKTPIPSRRLRSNNSPNKPVRI----T 120
            RRKTWKSDEGFN I IAEEKPES PSSGDEQ+RK+PIP+RRLRSN SPNKPV+I    T
Sbjct: 61  GRRKTWKSDEGFNPILIAEEKPESSPSSGDEQSRKSPIPARRLRSNGSPNKPVQISGEKT 120

Query: 121 ERNSTRKKTDQSRKSAVGIIKPPTNTNGIRKISPEKT-----------VSSPPPQDFFDA 180
           E+NSTRKKTDQSRKSAVG+ KPPTN  GI K  PEK+           +SS P QDFFDA
Sbjct: 121 EKNSTRKKTDQSRKSAVGLTKPPTN--GIGKTPPEKSFKELNECREKVISSAPSQDFFDA 180

Query: 181 FEE-IKKESFDVKEINFPAQE-----IFPGNKQKLQRLMDLIMWREFSRSAMAFGAGNLV 240
           FEE  +KESFDVKEIN P ++      FP NKQKLQ LMDLIMWR+FSRSA+ FG GNLV
Sbjct: 181 FEEEFEKESFDVKEINLPERKKIVAKTFPRNKQKLQTLMDLIMWRDFSRSALVFGVGNLV 240

Query: 241 IALSYFIKNMNISFISMISHMGLVYLAAIFVHSSIFSRGNGKGIDSDDEHCVVEEE-VIR 300
           I LSYFIKN+NISF+S+ISHMGL+YLAAIFVH SIF R   KGIDS+DE+  VEEE +IR
Sbjct: 241 IILSYFIKNINISFMSLISHMGLLYLAAIFVHRSIFGRR--KGIDSNDENYAVEEENMIR 300

Query: 301 LVKGFVPLVNELLQNLKPLFHGDPSATMKMGVLLFVLAKWGSFITFWNILQCGFVAVFTL 360
             K  VP VNELLQNLK LF GDPSATMK+GVLLFVLAKWGSFIT WN+L+ GF+ VFT+
Sbjct: 301 FAKRLVPFVNELLQNLKALFRGDPSATMKVGVLLFVLAKWGSFITLWNVLKIGFIGVFTV 360

Query: 361 PKLFTHGEHWLRLCGSSWQLSSHKKGMIVAIFLMVWNFSSILYRVWAAFIAFVSFRYYRE 413
           PKL THGEHWL+LCGSSW+L SHKKG+ VAIF +VWNFSSILYRVWAAFI FVS RYY+E
Sbjct: 361 PKLITHGEHWLKLCGSSWKLCSHKKGVFVAIFFLVWNFSSILYRVWAAFIVFVSVRYYQE 420

BLAST of Csor.00g270490 vs. ExPASy TrEMBL
Match: A0A6J1FIG4 (Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111444421 PE=4 SV=1)

HSP 1 Score: 811 bits (2095), Expect = 3.06e-296
Identity = 410/418 (98.09%), Postives = 413/418 (98.80%), Query Frame = 0

Query: 1   MDLSRRRAAVARGNVVAGSVWESRIRFDEVRGGIRVFNGDDETAECRVPAATVGSARRKT 60
           MDLSRRRAAVARGNVVAGSVWESRIRFDEVRGGIRVFNGDDET ECRVPAATVGSARRKT
Sbjct: 1   MDLSRRRAAVARGNVVAGSVWESRIRFDEVRGGIRVFNGDDETVECRVPAATVGSARRKT 60

Query: 61  WKSDEGFNSIQIAEEKPESPSSGDEQNRKTPIPSRRLRSNNSPNKPVRITERNSTRKKTD 120
           WKSDEGFNSIQIAEEKPESPSSGDEQNRKTPIPSRRLRSNNSPNKPVRITERNSTRKK D
Sbjct: 61  WKSDEGFNSIQIAEEKPESPSSGDEQNRKTPIPSRRLRSNNSPNKPVRITERNSTRKKID 120

Query: 121 QSRKSAVGIIKPPTNTNGIRKISPEKTVSSPPPQDFFDAFEEIKKESFDVKEINFPAQEI 180
           QSRKSAVGI KPPTNTNGIRKISP+KTVSSPPPQDFFDAFEEIK ESFDVKEINFPAQEI
Sbjct: 121 QSRKSAVGITKPPTNTNGIRKISPDKTVSSPPPQDFFDAFEEIKNESFDVKEINFPAQEI 180

Query: 181 FPGNKQKLQRLMDLIMWREFSRSAMAFGAGNLVIALSYFIKNMNISFISMISHMGLVYLA 240
           FPGNKQKLQRLMDLIMWREFSRSAMAFGAGNLVIALSYFIKNMNISFISMISHMGLVYLA
Sbjct: 181 FPGNKQKLQRLMDLIMWREFSRSAMAFGAGNLVIALSYFIKNMNISFISMISHMGLVYLA 240

Query: 241 AIFVHSSIFSRGNGKGIDSDDEHCVVEEEVIRLVKGFVPLVNELLQNLKPLFHGDPSATM 300
           AIFVHSSIFSRGNGKGIDSDDEHCVVEEEVIRLV+GFVP VNELLQNLKPLFHGDPSAT+
Sbjct: 241 AIFVHSSIFSRGNGKGIDSDDEHCVVEEEVIRLVRGFVPFVNELLQNLKPLFHGDPSATL 300

Query: 301 KMGVLLFVLAKWGSFITFWNILQCGFVAVFTLPKLFTHGEHWLRLCGSSWQLSSHKKGMI 360
           KMGVLLFVLAKWGSFITFWNILQCGFVAVFTLPKLFTHGEHWLRLCGSSWQLSSHKKGMI
Sbjct: 301 KMGVLLFVLAKWGSFITFWNILQCGFVAVFTLPKLFTHGEHWLRLCGSSWQLSSHKKGMI 360

Query: 361 VAIFLMVWNFSSILYRVWAAFIAFVSFRYYRESLGRDGWVKNGEAARHNDTVSGKFKA 418
           VAIFLMVWNFSSILYRVWAAFIAFVSFRYYRESLGRDGWVKNGEAARHNDTVSGKFKA
Sbjct: 361 VAIFLMVWNFSSILYRVWAAFIAFVSFRYYRESLGRDGWVKNGEAARHNDTVSGKFKA 418

BLAST of Csor.00g270490 vs. ExPASy TrEMBL
Match: A0A6J1HPY2 (Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111465561 PE=4 SV=1)

HSP 1 Score: 800 bits (2065), Expect = 1.14e-291
Identity = 405/417 (97.12%), Postives = 408/417 (97.84%), Query Frame = 0

Query: 1   MDLSRRRAAVARGNVVAGSVWESRIRFDEVRGGIRVFNGDDETAECRVPAATVGSARRKT 60
           MDLSRRRAAVARGNVVAGSVWESRIRFDEVRGGIRVFNGDDETAECRVPAATVGSARRKT
Sbjct: 1   MDLSRRRAAVARGNVVAGSVWESRIRFDEVRGGIRVFNGDDETAECRVPAATVGSARRKT 60

Query: 61  WKSDEGFNSIQIAEEKPESPSSGDEQNRKTPIPSRRLRSNNSPNKPVRITERNSTRKKTD 120
           WKSDE FNSIQIAEEKPESPSSGDE NRKTPIPSRRLRSNNSPNKPVRITERNSTRKKTD
Sbjct: 61  WKSDESFNSIQIAEEKPESPSSGDEPNRKTPIPSRRLRSNNSPNKPVRITERNSTRKKTD 120

Query: 121 QSRKSAVGIIKPPTNTNGIRKISPEKTVSSPPPQDFFDAFEEIKKESFDVKEINFPAQEI 180
           Q RKSAVGI KPP NTNGIRKISPEKTVSSPPPQDFFDAFEEIK ESFDVKEINFP QEI
Sbjct: 121 QPRKSAVGITKPPMNTNGIRKISPEKTVSSPPPQDFFDAFEEIKNESFDVKEINFPGQEI 180

Query: 181 FPGNKQKLQRLMDLIMWREFSRSAMAFGAGNLVIALSYFIKNMNISFISMISHMGLVYLA 240
           FPGNKQKLQRLMDLIMWREFSRSAMAFGAGNLVIALSYFIKNMNISFISMISHMGLVYLA
Sbjct: 181 FPGNKQKLQRLMDLIMWREFSRSAMAFGAGNLVIALSYFIKNMNISFISMISHMGLVYLA 240

Query: 241 AIFVHSSIFSRGNGKGIDSDDEHCVVEEEVIRLVKGFVPLVNELLQNLKPLFHGDPSATM 300
           AIFVHSSIFSRGNGKGIDSDDEHCVVEEEVIRLVKGFVP VNELLQNLKPLFHGDPSATM
Sbjct: 241 AIFVHSSIFSRGNGKGIDSDDEHCVVEEEVIRLVKGFVPFVNELLQNLKPLFHGDPSATM 300

Query: 301 KMGVLLFVLAKWGSFITFWNILQCGFVAVFTLPKLFTHGEHWLRLCGSSWQLSSHKKGMI 360
           KMG+LLFVLAKWGSFITFWNILQCGFVAVFTLPKLFTHGEHWLRLC SSWQLSSHKKGMI
Sbjct: 301 KMGLLLFVLAKWGSFITFWNILQCGFVAVFTLPKLFTHGEHWLRLCESSWQLSSHKKGMI 360

Query: 361 VAIFLMVWNFSSILYRVWAAFIAFVSFRYYRESLGRDGWVKNGEAARHNDTVSGKFK 417
           VAIFLMVWNFSSILYRVWAAFIAFVSFRYY+ESLGRDGW+KNGEAARHNDTVSGKFK
Sbjct: 361 VAIFLMVWNFSSILYRVWAAFIAFVSFRYYQESLGRDGWLKNGEAARHNDTVSGKFK 417

BLAST of Csor.00g270490 vs. ExPASy TrEMBL
Match: A0A1S3C3D4 (Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103496431 PE=4 SV=1)

HSP 1 Score: 584 bits (1505), Expect = 6.10e-206
Identity = 326/446 (73.09%), Postives = 355/446 (79.60%), Query Frame = 0

Query: 1   MDLSRRRAAVA-RGNVVAGSVWESRIRFDEVRGGIRVFNGDDETAECRVP-----AATVG 60
           MDLSRRRAAVA RGNVVAGSVWESRIRFDEVRGGI+VFNGDDE AEC VP     AA  G
Sbjct: 1   MDLSRRRAAVAARGNVVAGSVWESRIRFDEVRGGIKVFNGDDENAECGVPTTAASAAAGG 60

Query: 61  SARRKTWKSDEGFNSIQIAEEKPES-PSSGDEQNRKTPIPSRRLRSNNSPNKPVRI---- 120
           S RRKTWKSDEGFN I IAEEKPES PSSGDEQ+RK+P PSRRLRSN SPNKPV++    
Sbjct: 61  SGRRKTWKSDEGFNPILIAEEKPESSPSSGDEQSRKSPTPSRRLRSNGSPNKPVQVSGEK 120

Query: 121 TERNSTRKKTDQSRKSAVGIIKPPTNTNGIRKISPEKT-----------VSSPPPQDFFD 180
           TERNSTRKKTDQSRKS VG+ KPPTN  GI K S EK+           +SS   QDFFD
Sbjct: 121 TERNSTRKKTDQSRKSVVGLTKPPTN--GIGKTSSEKSFKELNECKEKVISSSTSQDFFD 180

Query: 181 AFEE-IKKESFDVKEINFPAQ-----EIFPGNKQKLQRLMDLIMWREFSRSAMAFGAGNL 240
           AFEE I+KESFDVKEIN P +     + FPGNKQKLQ LMDLIMWR+FSRS + FG GNL
Sbjct: 181 AFEEEIEKESFDVKEINLPERKKIIAQTFPGNKQKLQTLMDLIMWRDFSRSGLVFGVGNL 240

Query: 241 VIALSYFIKNMNISFISMISHMGLVYLAAIFVHSSIFSRGNGKGIDSDDEHCVVEEE-VI 300
           VI LS FIKN+NISFIS+ISHMGL+YL AIFVHSSIF R   K IDSDDE+ VVEEE +I
Sbjct: 241 VIILSCFIKNINISFISLISHMGLLYLTAIFVHSSIFGRR--KRIDSDDENFVVEEEDMI 300

Query: 301 RLVKGFVPLVNELLQNLKPLFHGDPSATMKMGVLLFVLAKWGSFITFWNILQCGFVAVFT 360
           R  K  VP VNELLQNLK LF GD SATMK+GVLLFVLAKWGSFIT WN+L+ GF+ VFT
Sbjct: 301 RFAKRSVPFVNELLQNLKALFRGDSSATMKVGVLLFVLAKWGSFITLWNVLKIGFIGVFT 360

Query: 361 LPKLFTHGEHWLRLCGSSWQLSSHKKGMIVAIFLMVWNFSSILYRVWAAFIAFVSFRYYR 417
           LPKL THGEHWL+LCGSSW+L SHK+G+ VAIF +VWNFSS LYRVWAAFI  VSFRYY+
Sbjct: 361 LPKLITHGEHWLKLCGSSWKLCSHKRGVFVAIFFLVWNFSSTLYRVWAAFIVLVSFRYYQ 420

BLAST of Csor.00g270490 vs. ExPASy TrEMBL
Match: A0A5A7V8P0 (Reticulon-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G001050 PE=4 SV=1)

HSP 1 Score: 582 bits (1501), Expect = 2.48e-205
Identity = 325/446 (72.87%), Postives = 354/446 (79.37%), Query Frame = 0

Query: 1   MDLSRRRAAVA-RGNVVAGSVWESRIRFDEVRGGIRVFNGDDETAECRVP-----AATVG 60
           MDLSRRRAAVA RGNVVAGSVWESRIRFDEVRGGI+VFNGDDE AEC VP     AA  G
Sbjct: 1   MDLSRRRAAVAARGNVVAGSVWESRIRFDEVRGGIKVFNGDDENAECGVPTTAASAAAGG 60

Query: 61  SARRKTWKSDEGFNSIQIAEEKPES-PSSGDEQNRKTPIPSRRLRSNNSPNKPVRI---- 120
           S RRKTWKSDEGFN I IAEEKPES PSSGDEQ+RK+P PSRRLRSN SPNKPV++    
Sbjct: 61  SGRRKTWKSDEGFNPILIAEEKPESSPSSGDEQSRKSPTPSRRLRSNGSPNKPVQVSGEK 120

Query: 121 TERNSTRKKTDQSRKSAVGIIKPPTNTNGIRKISPEKT-----------VSSPPPQDFFD 180
           TERNSTRKKTDQSRKS V + KPPTN  GI K S EK+           +SS   QDFFD
Sbjct: 121 TERNSTRKKTDQSRKSVVALTKPPTN--GIGKTSSEKSFKELNECKEKVISSSTSQDFFD 180

Query: 181 AFEE-IKKESFDVKEINFPAQ-----EIFPGNKQKLQRLMDLIMWREFSRSAMAFGAGNL 240
           AFEE I+KESFDVKEIN P +     + FPGNKQKLQ LMDLIMWR+FSRS + FG GNL
Sbjct: 181 AFEEEIEKESFDVKEINLPERKKIIAQTFPGNKQKLQTLMDLIMWRDFSRSGLVFGVGNL 240

Query: 241 VIALSYFIKNMNISFISMISHMGLVYLAAIFVHSSIFSRGNGKGIDSDDEHCVVEEE-VI 300
           VI LS FIKN+NISFIS+ISHMGL+YL AIFVHSSIF R   K IDSDDE+ VVEEE +I
Sbjct: 241 VIILSCFIKNINISFISLISHMGLLYLTAIFVHSSIFGRR--KRIDSDDENFVVEEEDMI 300

Query: 301 RLVKGFVPLVNELLQNLKPLFHGDPSATMKMGVLLFVLAKWGSFITFWNILQCGFVAVFT 360
           R  K  VP VNELLQNLK LF GD SATMK+GVLLFVLAKWGSFIT WN+L+ GF+ VFT
Sbjct: 301 RFAKRLVPFVNELLQNLKALFRGDSSATMKVGVLLFVLAKWGSFITLWNVLKIGFIGVFT 360

Query: 361 LPKLFTHGEHWLRLCGSSWQLSSHKKGMIVAIFLMVWNFSSILYRVWAAFIAFVSFRYYR 417
           LPKL THGEHWL+LCGSSW+L SHK+G+ VAIF +VWNFSS LYRVWAAFI  VSFRYY+
Sbjct: 361 LPKLITHGEHWLKLCGSSWKLCSHKRGVFVAIFFLVWNFSSTLYRVWAAFIVLVSFRYYQ 420

BLAST of Csor.00g270490 vs. ExPASy TrEMBL
Match: A0A0A0LG57 (Reticulon-like protein OS=Cucumis sativus OX=3659 GN=Csa_2G009500 PE=4 SV=1)

HSP 1 Score: 580 bits (1495), Expect = 1.82e-204
Identity = 319/443 (72.01%), Postives = 355/443 (80.14%), Query Frame = 0

Query: 1   MDLSRRRAAVA-RGNVVAGSVWESRIRFDEVRGGIRVFNGDDETAECRVP--AATVGSAR 60
           MDLSRRRAAVA +GNVVAGSVWESRIRFDEVRGGI+VFNGDDE  EC +P  AA+ GS R
Sbjct: 1   MDLSRRRAAVAAKGNVVAGSVWESRIRFDEVRGGIKVFNGDDENVECGIPTTAASAGSGR 60

Query: 61  RKTWKSDEGFNSIQIAEEKPES-PSSGDEQNRKTPIPSRRLRSNNSPNKPVRIT----ER 120
           RKTWKSDEGFN I IAEEKPES PSSGDEQ+RK+P PSRRLRSN+SPNKPV+++    ER
Sbjct: 61  RKTWKSDEGFNPILIAEEKPESSPSSGDEQSRKSPTPSRRLRSNSSPNKPVQVSGEKIER 120

Query: 121 NSTRKKTDQSRKSAVGIIKPPTNTNGIRKISPEKT-----------VSSPPPQDFFDAFE 180
           NSTRKKTDQSRKSAVG+ KPPTN  GI K S EK+           +SS   QDFFDAFE
Sbjct: 121 NSTRKKTDQSRKSAVGLTKPPTN--GIGKTSSEKSFKESNECREKVISSSTSQDFFDAFE 180

Query: 181 E-IKKESFDVKEINFPAQ-----EIFPGNKQKLQRLMDLIMWREFSRSAMAFGAGNLVIA 240
           E I+KESFDVKEIN P +     + FPGNKQKLQ LMDLIMWR+FSRS + FG GNLVI 
Sbjct: 181 EEIEKESFDVKEINLPERKKIIAQTFPGNKQKLQTLMDLIMWRDFSRSGLVFGVGNLVII 240

Query: 241 LSYFIKNMNISFISMISHMGLVYLAAIFVHSSIFSRGNGKGIDSDDEHCVVEEE-VIRLV 300
           LS FIKN+NIS IS+ISHMGL+YL  IFVHSSIF R   K IDS+DE+ VVEEE +IR  
Sbjct: 241 LSCFIKNINISLISLISHMGLLYLTTIFVHSSIFGRR--KRIDSNDENLVVEEEDMIRFA 300

Query: 301 KGFVPLVNELLQNLKPLFHGDPSATMKMGVLLFVLAKWGSFITFWNILQCGFVAVFTLPK 360
           K  VP VNELLQNLK LF GDPSATMK+GVLLFVLAKWGSFIT WN+L+ GF+ VFTLPK
Sbjct: 301 KRLVPFVNELLQNLKALFRGDPSATMKVGVLLFVLAKWGSFITLWNVLKIGFIGVFTLPK 360

Query: 361 LFTHGEHWLRLCGSSWQLSSHKKGMIVAIFLMVWNFSSILYRVWAAFIAFVSFRYYRESL 417
           L THGEHWL+LCGSSW+L SHK+G+ +AIF +VWNFSS LYRVWAAFI  VSFRYY+ES 
Sbjct: 361 LITHGEHWLKLCGSSWKLCSHKRGVFIAIFFLVWNFSSTLYRVWAAFIVLVSFRYYQESP 420

BLAST of Csor.00g270490 vs. TAIR 10
Match: AT5G58000.1 (Reticulon family protein )

HSP 1 Score: 188.3 bits (477), Expect = 1.2e-47
Identity = 122/352 (34.66%), Postives = 195/352 (55.40%), Query Frame = 0

Query: 59  KTWKSDEGFNSIQIAEEKPESPSSGDEQNRKTPIPSRRLRSNNSPNKPVRITERNSTRKK 118
           K+ ++++  N  Q   ++ +    GDE+        R +   +   K + + + N  R  
Sbjct: 113 KSNENEQIDNGDQEIGDQDDYEEDGDEEE------EREVEKKSVDVKEINVAKEN--RVG 172

Query: 119 TDQSRKSAVGIIKPPTNTNGIRKISP-----EKTVSSPPPQDFFDAFEEIKKESFDVKEI 178
             + +K +    +   + + +RKISP       +V S PP            + F  +E 
Sbjct: 173 GVEIKKFSQFQNRTSPSPSSVRKISPPVIKRATSVYSAPPN------STSSTDRFAEQED 232

Query: 179 NFPAQEIFPGNKQKLQRLMDLIMWREFSRSAMAFGAGNLVIALSYFIKNMNISFISMISH 238
           N      F  ++ KLQ L+DL+MWR+ SRS + FG G  +I  S +  ++N SFIS++++
Sbjct: 233 N------FTHSQSKLQSLVDLVMWRDVSRSTLVFGFGTFLIISSSYANDLNFSFISVVAY 292

Query: 239 MGLVYLAAIFVHSSIFSRGNGKGIDSDDEHCVV---EEEVIRLVKGFVPLVNELLQNLKP 298
           MGL+YL  +FV  S+  R    G+  ++ H VV   EE+V R+++  +P +NE L  L+ 
Sbjct: 293 MGLIYLGLMFVLKSLIHR----GMVEEERHKVVGVREEDVKRMLRLIMPYLNESLHQLRA 352

Query: 299 LFHGDPSATMKMGVLLFVLAKWGSFITFWNILQCGFVAVFTLPKLF--------THGEHW 358
           LF GDPS T+KMGV+LFVLA+ GS IT WN+ + GF+  FT+PK+F         +G  W
Sbjct: 353 LFSGDPSTTLKMGVVLFVLARCGSSITLWNLAKFGFLGAFTIPKIFISYSTHFSAYGNFW 412

Query: 359 LRLCGSSWQLSSHKKGMIVAIFLMVWNFSSILYRVWAAFIAFVSFRYYRESL 395
           +R    +W+  +HKK + +A+F +VWN SS+  RVWAAF+  V+FRYY+  +
Sbjct: 413 MRRFRDAWESCNHKKAVALALFTLVWNLSSVTARVWAAFMLLVAFRYYQHKM 440

BLAST of Csor.00g270490 vs. TAIR 10
Match: AT2G20590.1 (Reticulon family protein )

HSP 1 Score: 123.6 bits (309), Expect = 3.7e-28
Identity = 72/212 (33.96%), Postives = 113/212 (53.30%), Query Frame = 0

Query: 191 LMDLIMWREFSRSAMAFGAGNLVIALSYFIKNMNISFISMISHMGLVYLAAIFVHSSIFS 250
           + DL+MWR+ ++S + FG G L    S F K +N S  S +S++GLV L   F+ +++  
Sbjct: 168 ISDLVMWRDVAKSTLWFGFGCLSFLSSCFAKGVNFSVFSAVSNLGLVLLCGSFLSNTLCQ 227

Query: 251 RGNGKGIDSDDEHCVVEEEVIRLVKGFVPLVNELLQNLKPLFHGDPSATMKMGVLLFVLA 310
           R   K  D+     V E++V+R  +  +P  N  +     LF G+PS T+K+   L + A
Sbjct: 228 R---KNEDTKRAFHVSEDDVLRSARRVLPATNFFISKTSELFSGEPSMTLKVTPFLLLGA 287

Query: 311 KWGSFITFWNILQCGFVAVFTLPKLFTHGEHWL--------RLCGSSWQLSSHKKGMIVA 370
           ++G  IT W +   GF   FT+PKL++   H +           G +W + SHKK +  +
Sbjct: 288 EYGHLITLWRLSAFGFFLSFTIPKLYSCYTHQISQKVERVKTRIGEAWGVCSHKKILAGS 347

Query: 371 IFLMVWNFSSILYRVWAAFIAFVSFRYYRESL 395
                WN +SI  R++A FI  V FRY R++L
Sbjct: 348 AVTAFWNLTSIRTRIFAVFIILVIFRYRRQNL 376

BLAST of Csor.00g270490 vs. TAIR 10
Match: AT2G20590.2 (Reticulon family protein )

HSP 1 Score: 95.9 bits (237), Expect = 8.2e-20
Identity = 52/151 (34.44%), Postives = 83/151 (54.97%), Query Frame = 0

Query: 191 LMDLIMWREFSRSAMAFGAGNLVIALSYFIKNMNISFISMISHMGLVYLAAIFVHSSIFS 250
           + DL+MWR+ ++S + FG G L    S F K +N S  S +S++GLV L   F+ +++  
Sbjct: 168 ISDLVMWRDVAKSTLWFGFGCLSFLSSCFAKGVNFSVFSAVSNLGLVLLCGSFLSNTLCQ 227

Query: 251 RGNGKGIDSDDEHCVVEEEVIRLVKGFVPLVNELLQNLKPLFHGDPSATMKMGVLLFVLA 310
           R   K  D+     V E++V+R  +  +P  N  +     LF G+PS T+K+   L + A
Sbjct: 228 R---KNEDTKRAFHVSEDDVLRSARRVLPATNFFISKTSELFSGEPSMTLKVTPFLLLGA 287

Query: 311 KWGSFITFWNILQCGFVAVFTLPKLFTHGEH 342
           ++G  IT W +   GF   FT+PKL++   H
Sbjct: 288 EYGHLITLWRLSAFGFFLSFTIPKLYSCYTH 315

BLAST of Csor.00g270490 vs. TAIR 10
Match: AT4G28430.1 (Reticulon family protein )

HSP 1 Score: 95.1 bits (235), Expect = 1.4e-19
Identity = 91/358 (25.42%), Postives = 166/358 (46.37%), Query Frame = 0

Query: 72  IAEEKPESPSSGDEQNRKTPIPSRRLRSNNSPNKPVRITERNST--RKKTD--------- 131
           ++   P SP +  E    +P P R+ R+  S    +   E   T  R KT          
Sbjct: 54  VSLSSPSSPVTLREILLLSPSPLRKSRTRLSNRFDMEAAEAAVTARRSKTKGGQNGLLAS 113

Query: 132 -------QSRKSAVGIIKPPTNTNGIRKISPEKTVSSPPPQDFFDAFEEIKKESFDVKEI 191
                  +SR  +  ++    NT  I  ++ EK  +    +    + +E K  S  +   
Sbjct: 114 PSPRNFRRSRLRSEAMVDTKENTEPIVVVTDEKKQNQRKQKKLGRSKKE-KHSSVPLLAS 173

Query: 192 NFPA----QEIFPGNKQKL-QRLMDLIMWREFSRSAMAFGAGNL-VIALSYFIKNMNISF 251
             P+    Q++  G+ +++ + + DLIMWR+ ++S + FG G +  ++  +  K  N S 
Sbjct: 174 PSPSSDQPQDVCQGDLERIRENISDLIMWRDVAKSTLWFGFGCICFLSTCFAAKGFNFSV 233

Query: 252 ISMISHMGLVYLAAIFVHSSIFSRGNGKGIDSDDEHCVVEEEVIRLVKGFVPLVNELLQN 311
            S IS++GL++L   F+ +++  R      ++  E  + E++V+R+ +  +P+ N  +  
Sbjct: 234 FSAISYLGLLFLGVSFLSNTLRQRVTE---EARRELKLSEDDVLRIARRMLPITNLAISK 293

Query: 312 LKPLFHGDPSATMKMGVLLFVLAKWGSFITFWNILQCGFVAVFTLPKLFTHGEHWL---- 371
              LF G+P+ T+K+   + + A++G  IT W +   GF   FT+PKL++     L    
Sbjct: 294 TSELFSGEPAMTLKVAPFVLMGAEYGYLITLWRLCAFGFFLSFTIPKLYSCYASQLNQKV 353

Query: 372 ----RLCGSSWQLSSHKKGMIVAIFLMVWNFSSILYRVWAAFIAFVSFRYYRESLGRD 398
               R    +W + +HKK +  +     WN +S+  R  A FI  V  RY R++L  D
Sbjct: 354 ECAQRRFVEAWGVCTHKKFVAGSAVTAFWNLTSLKTRFIAVFIIVVVIRYRRQNLQLD 407

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q56X721.7e-4634.66Reticulon-like protein B21 OS=Arabidopsis thaliana OX=3702 GN=RTNLB21 PE=2 SV=2[more]
Q6DR045.2e-2733.96Reticulon-like protein B17 OS=Arabidopsis thaliana OX=3702 GN=RTNLB17 PE=2 SV=1[more]
Q8LDS32.0e-1825.42Reticulon-like protein B18 OS=Arabidopsis thaliana OX=3702 GN=RTNLB18 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
KAG6586339.15.90e-301100.00Reticulon-like protein B21, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022938288.16.32e-29698.09reticulon-like protein B21 [Cucurbita moschata][more]
XP_022965768.12.36e-29197.12reticulon-like protein B21 [Cucurbita maxima][more]
KAG7021185.11.46e-24686.36Reticulon-like protein B21, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_038890790.12.23e-20873.47reticulon-like protein B21 [Benincasa hispida] >XP_038890791.1 reticulon-like pr... [more]
Match NameE-valueIdentityDescription
A0A6J1FIG43.06e-29698.09Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111444421 PE=4 SV=1[more]
A0A6J1HPY21.14e-29197.12Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111465561 PE=4 SV=1[more]
A0A1S3C3D46.10e-20673.09Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103496431 PE=4 SV=1[more]
A0A5A7V8P02.48e-20572.87Reticulon-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2... [more]
A0A0A0LG571.82e-20472.01Reticulon-like protein OS=Cucumis sativus OX=3659 GN=Csa_2G009500 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G58000.11.2e-4734.66Reticulon family protein [more]
AT2G20590.13.7e-2833.96Reticulon family protein [more]
AT2G20590.28.2e-2034.44Reticulon family protein [more]
AT4G28430.11.4e-1925.42Reticulon family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003388ReticulonPFAMPF02453Reticuloncoord: 192..337
e-value: 5.4E-24
score: 84.9
IPR003388ReticulonPROSITEPS50845RETICULONcoord: 191..336
score: 14.341197
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 110..124
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 84..109
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 128..150
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 53..153
NoneNo IPR availablePANTHERPTHR46626:SF1RETICULON-LIKE PROTEIN B21coord: 1..122
coord: 162..406
IPR044647Reticulon-like protein B17/18/21PANTHERPTHR46626RETICULON-LIKE PROTEIN B17coord: 1..122
coord: 162..406

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g270490.m01Csor.00g270490.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane