Csor.00g269700 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g269700
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	tRNA (guanine(37)-N1)-methyltransferase
Location	Csor_Chr12: 15748546 .. 15753300 (-)
RNA-Seq Expression	Csor.00g269700
Synteny	Csor.00g269700

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGGACGAAAGCAAATTCGACGTGCATTTGAAACTCTGGGCGCTAAGAATTCCCAGGGAACTCTGTAAATCCGCCATTAAAATACTGAACGGGTACGTCTTGTTTTTTGTTGCTTAGTTTCTTAATTGTACATTTCTTCGTTTCTTAGTTGAACTGTTCATCGTTCTCATCCGAGTTCGACAATCTCTATTTTCTTGAAAATATTGTTCCGATATTGTTCTTCGGTCTCAGTTCGTTTCTTTTGTAAAATTTTTAGGTACTTAATTGATAGGCCCCGGATTAAACCTGTGACGGAAGATCCAACATGTGATAAGAATCGGTATGTTATATTATCGGAAAAAGTTCAATCTACAGGTAAAATTCTCTGTCCTTGCACTTACTGACTTGGTTTTCTCTGTTCATCATGCTCTTTCATAACACTTTCTGTGAGCCAAAGCTCTTTAAAATGGCATCCTACGTTTCTTCTTCCCTTTTTGGTCCTTAGATGACACCTTTTTCTATCTTGTGATTCTTTGAATGGCTCAGAGTTGTCCGAAATTCCAGAAATGAAGCTTGAAGAATTGAAAGGTTTATGCGAGATCGAACTAGTTCCTTATTCACTAACTCTTGGATATTCTTATTGGGGAACAGGTAGTTTCAATAATCCCATCTTTTATTTTACGCATCGTGCAAGTTTGACTTGACGCATCGTGTGTAACGACCCTATTTTTCTTTATAAAATAGAGATGTCACTACATGCATGACGTGTCTAATATGTGTGTAAAAGTCTGAAAATATTTCATAACGATGCCATGGACGTAAGTGTTTATAATAAGTTCATTAAAACAATATTGTTATGAACAACATAGCGGAAAATAGATAATATGAATTTAAATAAAAAAAAACGAGACAATGCCCTGATCTAACTTGAGGGCTACTAAAACCTACTATTCTAGGTATGGTCATGTGACCTTTACTACAATGTCGTTTTCCGTACATGGATGCCTTACCTTAACTTGAAAAATAAGAGTAGCACATCACTTGAGTATTTTAAGATATATTCAGGAAGTAGTCTCATTATTGAGGTCGGGAAATGCAAACACAAGCAATCATGTTGGGACCTTTCATTGCGTAAAAACATCATGTCATGACCTTGTTCTTGACTTCCAGTCCGGGAAGTGTTGTATGTTGTCTTTGACTAATATTATGATAACTGCATATACCGTGTCTACTTTTCAGTGATAGTTTAAACTATTATATATGTGCGTTTACTTGACATTTGATGTGTTCATCTTGTTCATGGCCGGCTAACTTAGCGTGTGGTTCTTATGTAGATCATGTACTGAAGCAGATTCTGCCTCATGGAGTGGAAGTGCCATCATCGTTTGAAACAATAGTTAAGTACTCAAGCCATTGCCATGTTTTGTCTTTCCTTTTCCTTTCTTTCTTACATTTGCTTGTGCTGTTTGTAGATTTTTACGTGCGTTCTTTCCTTGTTCGTTCATTACAACTATTGACAGGGAGGTGATGTTGTTCTGTGATTTTACAGGTCACGTAGCTCACCTTAATATACATGACGAATTGCTCCCTTATAAGGAAGTCATTGCGAAGGTTATTTACGATGTACGTCGTCAAGATCCATTTACTCATTTTTTTCCAACGAATTTGAAACGTAAATTGTGAATAAATGATCATCATTCTATAGTTGTTACCTCATTTTCAATGCTTTTAAGTGTCTTTGTGTAATAGCCCAAGCCCATCGCTAAACGATGTTGTCCTCTTTGGGCTTCCCTTTCGGGCTTTCCCTCAAGGTTTTTTGAACGCGTCTCCTAGGAGAGGTTTCTACACCTTTATAAAAAATGTTCCGTTCTCCCCAACCGATGTGGGATCTCACAATCCACCCCCTGGCCCAACGTCCTCACTGACACTCGTTCCCTTCTCCAATCGATGTGGGATCCCCCAATCTACTTCCCTTCGTGGCCCAGCGTCCTTGCTGGCACACTGCCTCGTTCCGCCCCCTCTTCAGGATTCAGCCTCCTTACTGGCATATTGGCCGAGGGTGTGTGTAGGGGGTACCCTTTTCAAAAATTCAATTGCTAATGATTTAGCAAGAATCAGATTTCTTTATTGCTTTTAGTGCCATTGTTGGTCCCTTTCCCCCTCATTTTCCCTATAACAACTTTTCTATAACTAATTCTTTTGAATATTACGTGGGGTATGGAGGTTGAAACCTACCTCTCTAGTAGAGAGTTGAAAATTTTTAATCGTTGAACTATGCTCGTTTATTCGTTTGTAATTATTTCTTTTCAAAATTTAATTGCAGAAAAATTATCCGAGAATCAAAACAGTGGTAAATAAAGTGGGGTCGATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAGTTCTGAAGGGAGAGAATGATATGGAAACAGAAGTGAAGCAATATGGAGCAACTTTTAAGCTTGACTACAGTTTGGTCTACTGGAACTCAAGATTAGAGCATGAACACATCAGACTGGTTTCACTGTTCCAACCAGGAGAGATAATTTGCGACATGTTCGCCGGTATTGGTCCTTTTGCTATTCCAGCTGCCCAGAAAGGCTGTATAGTATATGCCAATGACTTGAACCCGGATAGCATTCGATATCTGAAGATTAATGCAGAAATCAATAAAGTCGGTGATCGTGTTCATGTTCATAATTTGGACGCTAGGAGGTTCATTTCTCAATTGATGACCGTGCCAGACAATGAATGTAATTCGGAATCTGTCGCCTCAGTTTTAAAAGCTTGTGAGAAAGGTATCGGAGGGACCGCTTAGTATCATCATTGAAGGAAATTTTCATAATAGAGATCGTGTATCGTCGATCTTAATTTTCATTCTTGCTTCAGTTCAGGTGGAGGAAGTCCAAGGTAGTGTTAAAACTAGCGATGAAAGTGTAGAAGGTTCAAGGAGTGCTGATGTATCTGTTGCTGCTCTTAAAAGATCCTCAGAATGCTGTGAGAAAGGTAAGATACCCATCAAATCCAAGTAGGGCTATAAAAAATTCATTGTGGGAACTGAATATAGATGTTCTATATCTTTATTATAAATTAAATCTACTACACCTTTAGCAATTAAGACAGCTCGACGTTGAGAATTTATAGCATGTTGCTCATCACTGTATGTCTCTCCCACATCGGTTGGAGAGGAGAACGAAACATTTTTTATAAGGGTGTGGAAACCTCTCCCTAGCAGACACGTTTTAAGAACCTTAAGGGAAACCCCGAAAGGGAAAGTCCAAAGAGGACAATATCTGCTAGCGGTGAGCTTGGGCTGTTATTAAACGATGAAAGATCATTTCTTCTTAAAATTTGCGTTGCAGATAATGGAATAGCTGATGATGCTTCCATCTGTGTAGCTGGTAAGAGAAAAGGAAACAGTAACAAGAGGTTGAAGGGGTCCAAAATATCTCATGCTAAGACGTGGGAGCATGTTGATCATGTGATTATGAACCTCCCCGCTTCTGCTTTACATTTTCTCGGTATGAACGTTTTTTTTCGGGTTTATTTCATTTACCTTGTCGTGGTTGTTATGCATATTCCTATACATTTTTTAACGTTGGAGGAAGAATTGAAACTTGGGAAGTATCAATGTTAGCTAGCCCACATCTGTCGGTATGAGATTAGAGGGAAAATTATGGAAAGGGAAAGTCTATGTAACAACCCAAACCTACTATCAGCCGATATTGTCCTATCTGATTGTAACAACCCAAACTCACCGCTAGCAAATCCCACATCAGTTGATGAGGAGAACGAATCATTCTTTATAAGGGTGTGGAAACCTTTCTTTAGCAGATGCGTTTTAAAACCTTGATGGGAAGCCGAGAAGGGAAAGCCCAAAGAAGACAATGTCTGCTAGTGGTGGGTTTGGGCGGTTACAAATGGTATCCGAGCCAGACATCGGGCAATGTGCCAGCAAGGAGGCTGAACCCTGAAGGGGGTGAACATGAGACGGTGTGCCAGCAAGGATGCTGGGCTCTGAAAGGGGGTTGATTAAGGAGTCCCACATCGATTGGAGAAGGGAACAAGTGTCAGCGAAGACGCTGGGCCTCGAAGGGGGTGGATTGTGAGATCTAACATCGGTTGGGGAAGAGAACGAAACATTCTTTATAAGGGTGTGGAAACCTCTCCCTAACAGATGCGTTTTAAAATCTTGTAGGGAAGCTCGGAAGGGAAAGCCTAAGAGAACAACATCTGCTAGCGGTAGGCTTAGGCTGTTACAATATTAGTATGCTACTCTTTTCGACATGTCATGCTCTTTTGTATCTGTAACGATAATTTGTTTGTTTAATCACCAAAGAATATTCGGCATTTTCTTACCAGATGCATTTAGGCGCTCGATTCCAAGGAAGTACTGGAAGGGCTCCCTTCCATGGATTCACTGTTATTGCTTCATGCGAGCAAATGAGAATCAAGAGTTCATCATTTCGGTATGTTCAGACTCCTATTTCGATATTCGTCTACCAAAATCATCCTTCTTCTCATGCTATCTTCTTAACTGTTCATCGCAGGTAGCCGAGTCTGCCTTGAATGCTAAAATCCAAGACCCTATTTTTCACAGAGTCAGAGACGTTGCACCAAACAAGGTATTGCTCTCTATTTGTTCTTTTAGCTTACTTCCATTGTTCTTTAACACTTGCCTTTTCTTTCTTATGTTCTTTGTTTTGATAGGCAATGTATTGTTTAAGCTTTAGGCTTCCACAACAATGCTTCGACGAAGAGTTGGCTAGCTGA

mRNA sequence

ATGTTGGACGAAAGCAAATTCGACGTGCATTTGAAACTCTGGGCGCTAAGAATTCCCAGGGAACTCTGTAAATCCGCCATTAAAATACTGAACGGGTACTTAATTGATAGGCCCCGGATTAAACCTGTGACGGAAGATCCAACATGTGATAAGAATCGGTATGTTATATTATCGGAAAAAGTTCAATCTACAGAGTTGTCCGAAATTCCAGAAATGAAGCTTGAAGAATTGAAAGGTTTATGCGAGATCGAACTAGTTCCTTATTCACTAACTCTTGGATATTCTTATTGGGGAACAGATCATGTACTGAAGCAGATTCTGCCTCATGGAGTGGAAGTGCCATCATCGTTTGAAACAATAGTTAAGGAGGTGATGTTGTTCTGTGATTTTACAGGTCACGTAGCTCACCTTAATATACATGACGAATTGCTCCCTTATAAGGAAGTCATTGCGAAGGTTATTTACGATAAAAATTATCCGAGAATCAAAACAGTGGTAAATAAAGTGGGGTCGATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAGTTCTGAAGGGAGAGAATGATATGGAAACAGAAGTGAAGCAATATGGAGCAACTTTTAAGCTTGACTACAGTTTGGTCTACTGGAACTCAAGATTAGAGCATGAACACATCAGACTGGTTTCACTGTTCCAACCAGGAGAGATAATTTGCGACATGTTCGCCGGTATTGGTCCTTTTGCTATTCCAGCTGCCCAGAAAGGCTGTATAGTATATGCCAATGACTTGAACCCGGATAGCATTCGATATCTGAAGATTAATGCAGAAATCAATAAAGTCGGTGATCGTGTTCATGTTCATAATTTGGACGCTAGGAGGTTCATTTCTCAATTGATGACCGTGCCAGACAATGAATGTAATTCGGAATCTGTCGCCTCAGTTTTAAAAGCTTGTGAGAAAGTTCAGGTGGAGGAAGTCCAAGGTAGTGTTAAAACTAGCGATGAAAGTGTAGAAGGTTCAAGGAGTGCTGATGTATCTGTTGCTGCTCTTAAAAGATCCTCAGAATGCTGTGAGAAAGCTGGTAAGAGAAAAGGAAACAGTAACAAGAGGTTGAAGGGGTCCAAAATATCTCATGCTAAGACGTGGGAGCATGTTGATCATGTGATTATGAACCTCCCCGCTTCTGCTTTACATTTTCTCGATGCATTTAGGCGCTCGATTCCAAGGAAGTACTGGAAGGGCTCCCTTCCATGGATTCACTGTTATTGCTTCATGCGAGCAAATGAGAATCAAGAGTTCATCATTTCGGTAGCCGAGTCTGCCTTGAATGCTAAAATCCAAGACCCTATTTTTCACAGAGTCAGAGACGTTGCACCAAACAAGGCAATGTATTGTTTAAGCTTTAGGCTTCCACAACAATGCTTCGACGAAGAGTTGGCTAGCTGA

Coding sequence (CDS)

ATGTTGGACGAAAGCAAATTCGACGTGCATTTGAAACTCTGGGCGCTAAGAATTCCCAGGGAACTCTGTAAATCCGCCATTAAAATACTGAACGGGTACTTAATTGATAGGCCCCGGATTAAACCTGTGACGGAAGATCCAACATGTGATAAGAATCGGTATGTTATATTATCGGAAAAAGTTCAATCTACAGAGTTGTCCGAAATTCCAGAAATGAAGCTTGAAGAATTGAAAGGTTTATGCGAGATCGAACTAGTTCCTTATTCACTAACTCTTGGATATTCTTATTGGGGAACAGATCATGTACTGAAGCAGATTCTGCCTCATGGAGTGGAAGTGCCATCATCGTTTGAAACAATAGTTAAGGAGGTGATGTTGTTCTGTGATTTTACAGGTCACGTAGCTCACCTTAATATACATGACGAATTGCTCCCTTATAAGGAAGTCATTGCGAAGGTTATTTACGATAAAAATTATCCGAGAATCAAAACAGTGGTAAATAAAGTGGGGTCGATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAGTTCTGAAGGGAGAGAATGATATGGAAACAGAAGTGAAGCAATATGGAGCAACTTTTAAGCTTGACTACAGTTTGGTCTACTGGAACTCAAGATTAGAGCATGAACACATCAGACTGGTTTCACTGTTCCAACCAGGAGAGATAATTTGCGACATGTTCGCCGGTATTGGTCCTTTTGCTATTCCAGCTGCCCAGAAAGGCTGTATAGTATATGCCAATGACTTGAACCCGGATAGCATTCGATATCTGAAGATTAATGCAGAAATCAATAAAGTCGGTGATCGTGTTCATGTTCATAATTTGGACGCTAGGAGGTTCATTTCTCAATTGATGACCGTGCCAGACAATGAATGTAATTCGGAATCTGTCGCCTCAGTTTTAAAAGCTTGTGAGAAAGTTCAGGTGGAGGAAGTCCAAGGTAGTGTTAAAACTAGCGATGAAAGTGTAGAAGGTTCAAGGAGTGCTGATGTATCTGTTGCTGCTCTTAAAAGATCCTCAGAATGCTGTGAGAAAGCTGGTAAGAGAAAAGGAAACAGTAACAAGAGGTTGAAGGGGTCCAAAATATCTCATGCTAAGACGTGGGAGCATGTTGATCATGTGATTATGAACCTCCCCGCTTCTGCTTTACATTTTCTCGATGCATTTAGGCGCTCGATTCCAAGGAAGTACTGGAAGGGCTCCCTTCCATGGATTCACTGTTATTGCTTCATGCGAGCAAATGAGAATCAAGAGTTCATCATTTCGGTAGCCGAGTCTGCCTTGAATGCTAAAATCCAAGACCCTATTTTTCACAGAGTCAGAGACGTTGCACCAAACAAGGCAATGTATTGTTTAAGCTTTAGGCTTCCACAACAATGCTTCGACGAAGAGTTGGCTAGCTGA

Protein sequence

MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEKVQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETIVKEVMLFCDFTGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGPFAIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDARRFISQLMTVPDNECNSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEKAGKRKGNSNKRLKGSKISHAKTWEHVDHVIMNLPASALHFLDAFRRSIPRKYWKGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQQCFDEELAS

Homology

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BLAST of Csor.00g269700 vs. ExPASy Swiss-Prot
Match: F6HH45 (tRNA (guanine(37)-N1)-methyltransferase 2 OS=Vitis vinifera OX=29760 GN=VIT_11s0016g04350 PE=3 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 3.9e-188
Identity = 338/504 (67.06%), Postives = 398/504 (78.97%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           +LDESKFD+ LKLWALRIP +LCK A +ILNGYL+D+PR+KP+TEDPTC+ NRY+ILSE+
Sbjct: 2   LLDESKFDLQLKLWALRIPSQLCKVASRILNGYLLDKPRVKPITEDPTCETNRYIILSER 61

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           +++ +LS+IPE K +ELK LC+IE+VPYSLTLGYSYW  DHVLKQILP GVEVPSSFETI
Sbjct: 62  IKNPDLSDIPEDKRDELKVLCKIEVVPYSLTLGYSYWSADHVLKQILPDGVEVPSSFETI 121

Query: 121 VKEVMLFCDFTGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK 180
              +        H+AHLNI  ELLPYK+VIAKVIYDKNYPRIKTVVNKVG+ITNEFRVPK
Sbjct: 122 TFSLC-------HIAHLNITGELLPYKDVIAKVIYDKNYPRIKTVVNKVGTITNEFRVPK 181

Query: 181 FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP 240
           FE+L G++DM TEVKQY ATFKLDYSLVYWNSRLEHEH+RLVS F+PG+IICDMF+G+GP
Sbjct: 182 FEILVGKDDMVTEVKQYRATFKLDYSLVYWNSRLEHEHMRLVSQFRPGQIICDMFSGVGP 241

Query: 241 FAIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDARRFISQLMTVPDNEC 300
           FAIPAAQKGC+VYANDLNPDSIRYLKINA+INKV D +  +N+DAR+FISQLM VP +E 
Sbjct: 242 FAIPAAQKGCLVYANDLNPDSIRYLKINAKINKVDDNIWAYNMDARKFISQLMEVPVHEI 301

Query: 301 NSESVASVLKACEK------VQVEEVQGSVKT------SDES-----VEGSRS-ADVSVA 360
             E    VLKA E+      VQ E   G +        SD+S     +EGS + AD +VA
Sbjct: 302 LPEPDVPVLKATEECSIQANVQTESQNGGLTVEAIGVHSDDSSNMDGIEGSCTVADDTVA 361

Query: 361 ALKRSSECCEK------------AGKRKGNSNKRLKGSK-ISHAKTWEHVDHVIMNLPAS 420
           A+K+ S  CE+             G+RK   NKR++GS+  S+ K WEHVDHVIMNLPAS
Sbjct: 362 AVKKHSGSCEEENGTYDNATVSVVGRRKDRMNKRMRGSESSSNIKPWEHVDHVIMNLPAS 421

Query: 421 ALHFLDAFRRSIPRKYWKGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVR 473
           A+ FLDAFR  I RKYWKGSLPWIHCYCF+RANE  E IIS AE+ALNA IQ+PI HRVR
Sbjct: 422 AIQFLDAFRGLIQRKYWKGSLPWIHCYCFIRANETNEMIISEAETALNASIQEPILHRVR 481

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BLAST of Csor.00g269700 vs. ExPASy Swiss-Prot
Match: Q93YU6 (tRNA (guanine(37)-N1)-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At3g56120 PE=2 SV=1)

HSP 1 Score: 628.2 bits (1619), Expect = 7.3e-179
Identity = 314/480 (65.42%), Postives = 375/480 (78.12%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           M DESKFDV+LKLWALRIPRELCKSA +ILNGY+++ PRIKP+TEDPTC+K R VILSE 
Sbjct: 1   MFDESKFDVNLKLWALRIPRELCKSASRILNGYMLNMPRIKPITEDPTCEKTRLVILSES 60

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           V++ +LSEIPE KL +LK L E+E+VP+S+TLGYSYW  DH+LKQILP G+++PSSFETI
Sbjct: 61  VKNADLSEIPEEKLNQLKKLSELEVVPHSVTLGYSYWSADHLLKQILPDGLDIPSSFETI 120

Query: 121 VKEVMLFCDFTGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK 180
                      GH+AHLN+HDELLP+K+VIAKVIYDKNYPRIKT+VNKVG+I+NEFRVPK
Sbjct: 121 -----------GHIAHLNLHDELLPFKDVIAKVIYDKNYPRIKTIVNKVGTISNEFRVPK 180

Query: 181 FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP 240
           FEVL GEN METEVKQYGA FKLDY LVYWNSRLEHEH+RL SLF+PGE +CDMFAGIGP
Sbjct: 181 FEVLAGENGMETEVKQYGARFKLDYGLVYWNSRLEHEHMRLSSLFKPGETVCDMFAGIGP 240

Query: 241 FAIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDARRFISQLMTVPDNEC 300
           FAIPAAQKGC VYANDLNPDS+RYLKINA+ NKV D + VHN+DAR+F S LM V   E 
Sbjct: 241 FAIPAAQKGCFVYANDLNPDSVRYLKINAKFNKVDDLICVHNMDARKFFSHLMAVSTCED 300

Query: 301 NSESVASVLKACEKVQVEEVQGSVKTSDESV-EGSRSADVSVAALKRSSECCEK---AGK 360
           N +SVA   K   K       G   +S E + E + S +  + A K+ S   +     GK
Sbjct: 301 NLQSVADNDKT--KEAAVSRGGETNSSGEEIRESNASINEPLGANKKPSGTTKTENGVGK 360

Query: 361 R----KGNSNKRLKGSKISHAKTWEHVDHVIMNLPASALHFLDAFRRSIPRKYWKGSLPW 420
                +G++NKRL+ + +  AK WEH+DHVIMNLPASAL FLD+F   I +KYWKG LP 
Sbjct: 361 DCKSIEGHANKRLRQTLLPIAKPWEHIDHVIMNLPASALQFLDSFSNVIQKKYWKGPLPL 420

Query: 421 IHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQQCFDEE 473
           IHCYCF+RA+E  EFII+ AE+AL   I+DP+FH+VRDVAPNKAM+CLSFRLP+ C  +E
Sbjct: 421 IHCYCFIRASETTEFIIAEAETALKFHIEDPVFHKVRDVAPNKAMFCLSFRLPEACLKQE 467

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BLAST of Csor.00g269700 vs. ExPASy Swiss-Prot
Match: C5XX79 (tRNA (guanine(37)-N1)-methyltransferase OS=Sorghum bicolor OX=4558 GN=Sb04g025390 PE=3 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 8.2e-154
Identity = 281/487 (57.70%), Postives = 347/487 (71.25%), Query Frame = 0


Query: 2   LDESKFDVHLKLWALRIPRELCKSAIKIL-NGYLIDRPRIKPVTEDPTCDKNRYVILSEK 61
           LDESKF+  L+LWALRIPREL  +  ++L +GYL+D+PR+K V EDP  DKNR VILSEK
Sbjct: 4   LDESKFEQRLQLWALRIPRELSSAVTRLLRSGYLLDKPRVKTVVEDPEGDKNRLVILSEK 63

Query: 62  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 121
           +Q  +LS++P+ +L+ LK LC +++VPY+LTLGYSYW                       
Sbjct: 64  IQKPDLSDMPQQELDSLKQLCNVDVVPYTLTLGYSYWS---------------------- 123

Query: 122 VKEVMLFCDFTGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK 181
                      GHVAHLNI D+LLPYK VIAKVIYDKNYPRI+TV NKVG+ITNEFRVPK
Sbjct: 124 ----------AGHVAHLNISDDLLPYKNVIAKVIYDKNYPRIQTVANKVGTITNEFRVPK 183

Query: 182 FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP 241
           FE+L G+NDM TEVKQYGATFKLDY LVYWNSRL+HEHIRLVSLF+ G++ICDMFAGIGP
Sbjct: 184 FEILAGKNDMVTEVKQYGATFKLDYGLVYWNSRLDHEHIRLVSLFKKGDVICDMFAGIGP 243

Query: 242 FAIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDARRFISQLMTVPDNEC 301
           FAIPAAQKGC+VYANDLNPDS+ YL+ NA+INKV D +  +N+DAR F+  LMTVP  E 
Sbjct: 244 FAIPAAQKGCVVYANDLNPDSVHYLRTNAKINKVDDYIFAYNMDARVFMQNLMTVPGLET 303

Query: 302 NSESVASVLKACEK--VQVEEVQGSVKTSDESVEGSR-----SADVSVAALKR--SSECC 361
            S+   +  ++  K  V   E   S    ++  EGS+     S+  S  A KR  +SE C
Sbjct: 304 GSDCQVAADESYPKEGVPANENSSSNGNHNDVREGSQNGANESSVASTTAKKRQQTSEEC 363

Query: 362 EK------AGKRKGNSNKRLKGSKISHAKTWEHVDHVIMNLPASALHFLDAFRRSIPRKY 421
           E       A + K  +NKR++G     +K WEH DHV+MNLPASAL FLD F   + +KY
Sbjct: 364 ESDCQDGDASQTKRRNNKRVRGPGPPPSKPWEHFDHVLMNLPASALQFLDCFDGLVQKKY 423

Query: 422 WKGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLP 473
           W GSLPWIHCYCF+R++E++E I+S   + LNAKI +PIFHRVRDVAPNKAM+CLSF+LP
Sbjct: 424 WTGSLPWIHCYCFIRSSESEESILS---NKLNAKIAEPIFHRVRDVAPNKAMFCLSFKLP 455

[Close]

BLAST of Csor.00g269700 vs. ExPASy Swiss-Prot
Match: A9T6G5 (tRNA (guanine(37)-N1)-methyltransferase OS=Physcomitrium patens OX=3218 GN=PHYPADRAFT_141055 PE=3 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 5.0e-119
Identity = 223/455 (49.01%), Postives = 302/455 (66.37%), Query Frame = 0


Query: 2   LDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEKV 61
           +D+S F V L   ALRIP++       +L+ YL+D PR+K + EDP     R ++ SE +
Sbjct: 11  IDKSAFVVRLPRVALRIPKQKSHRVASLLSKYLLDMPRVKHIVEDPQDPSMRLLLFSEAI 70

Query: 62  QSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETIV 121
              +L  + E K+  +K +  +++V + + L YSYW  +H+LK+ILP G EVPSSFETI 
Sbjct: 71  AQPDLEGVSEEKILAVKEIVPLDVVQHEVVLDYSYWPVEHILKEILPAGCEVPSSFETI- 130

Query: 122 KEVMLFCDFTGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKF 181
                     GH+AHLN+ ++LL YK++IA+VI DKN P++KTVVNKVG+ITNEFRVP+F
Sbjct: 131 ----------GHIAHLNLREDLLTYKKIIAEVILDKN-PKLKTVVNKVGTITNEFRVPEF 190

Query: 182 EVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGPF 241
           E+L GE  + TE+KQ+GATF+LDY +VYWNSRLE EH RL + F+PG++I DMFAGIGPF
Sbjct: 191 EILAGEPSLVTEIKQHGATFRLDYGMVYWNSRLEGEHKRLFAQFKPGQVIVDMFAGIGPF 250

Query: 242 AIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDARRFISQLMTVPDNECN 301
           AIPAAQ+GC VYANDLNP S+++LK+N++INKVG+ +   NLDAR F+ +L+T  +  C 
Sbjct: 251 AIPAAQQGCAVYANDLNPTSVKFLKLNSDINKVGESIKAFNLDAREFMRKLVT-EEEGCV 310

Query: 302 SESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEKAGKRKGNS 361
            E  +S L A   +    +  S + S    EGS      + + K+  E   KA K K + 
Sbjct: 311 GEIASSTLIAENSLNGTRIPSSKENSSSLTEGS-----DMPSSKKPEE--RKAKKAKVSE 370

Query: 362 NKRLKGSKISHAKTWEHVDHVIMNLPASALHFLDAFRRSIPRKYWKGSLPWIHCYCFMRA 421
             ++   KIS  K WEH DHV+MNLPASAL FLD     + +  WKG++P +HCYCFMR+
Sbjct: 371 VVKM---KISDVKPWEHFDHVVMNLPASALEFLDVLNGLLCKGRWKGTMPRVHCYCFMRS 430

Query: 422 NENQEFIISVAESALNAKIQDPIFHRVRDVAPNKA 457
           NE    I+  AE+ L   I DP  + VRDVAPNKA
Sbjct: 431 NETNADIVKKAETYLGGSIADPDVYVVRDVAPNKA 442

[Close]

BLAST of Csor.00g269700 vs. ExPASy Swiss-Prot
Match: F4NUJ6 (tRNA (guanine(37)-N1)-methyltransferase OS=Batrachochytrium dendrobatidis (strain JAM81 / FGSC 10211) OX=684364 GN=TRM5 PE=3 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 4.0e-76
Identity = 166/470 (35.32%), Postives = 258/470 (54.89%), Query Frame = 0


Query: 2   LDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEKV 61
           L+ + F+    L AL+IP      A++ L  +++  PR++ + +DPT  K R ++L   +
Sbjct: 16  LNHALFESSHTLPALKIPAHASGIAMETLRSHILVAPRLRTIVDDPTDKKWRLLLLDPSM 75

Query: 62  QSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETIV 121
           +++E++++P   L+E     E +LV +++ L Y YW +D VL+ ILP  +E P +FET+ 
Sbjct: 76  EASEINDLPN-PLKEFALKHEAKLVKHTIELKYDYWTSDQVLRSILPDEMETPGAFETV- 135

Query: 122 KEVMLFCDFTGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKF 181
                     GH+AHLN+ D   P+K +I +VI DK+   IKTVVNK+ +I + FR  + 
Sbjct: 136 ----------GHIAHLNLRDRYQPFKHIIGQVILDKS-SHIKTVVNKLDNIDHTFRFFQM 195

Query: 182 EVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGPF 241
           E+L G NDM  ++K+ G  F  D+S VYWNSRL+ EH R++ LF   ++ICD+FAG+GPF
Sbjct: 196 EILAGINDMNAKLKEGGCFFHFDFSKVYWNSRLQGEHDRIIKLFGQNDLICDVFAGVGPF 255

Query: 242 AIPAAQ-KGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDARRFISQLMTVPDNEC 301
           A+PAA+ K C+V+ANDLNP S +YL  N ++NK+  R+   N+D R+FI Q +     + 
Sbjct: 256 ALPAAKHKRCVVFANDLNPQSFKYLMENIKLNKLETRILPFNMDGRQFIKQSL----EDL 315

Query: 302 NSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEKAGKRKGN 361
           N+ ++        K+  ++   + K  +  VE    A ++                    
Sbjct: 316 NNPAI------WNKITKQKPTSNDKKRNRKVESPTVAPLT-------------------- 375

Query: 362 SNKRLKGSKISHAKTWEHVDHVIMNLPASALHFLDAFR---RSIPRKYWKGSLPWIHCYC 421
               + G +        H  H +MNLPA+A+ FLDAF      +        LP IHC+C
Sbjct: 376 DQPAISGIR--------HFKHYVMNLPATAIEFLDAFHGLYSGMRDVIMDSDLPTIHCHC 434

Query: 422 FMRANENQEFIISVAESALNAKIQDPIF--HRVRDVAPNKAMYCLSFRLP 466
           F  A + +  +I   E  +   +   +   H VR VAPNK M C+SFRLP
Sbjct: 436 FSNAKDVKADVIERVERVIGMPLGSNLIMVHSVRTVAPNKDMLCISFRLP 434

[Close]

BLAST of Csor.00g269700 vs. NCBI nr
Match: KAG6586416.1 (tRNA (guanine(37)-N1)-methyltransferase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 965 bits (2495), Expect = 0.0
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI
Sbjct: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120

Query: 121 VKEVMLFCDFTGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK 180
           VKEVMLFCDFTGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK
Sbjct: 121 VKEVMLFCDFTGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK 180

Query: 181 FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP 240
           FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP
Sbjct: 181 FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP 240

Query: 241 FAIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDARRFISQLMTVPDNEC 300
           FAIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDARRFISQLMTVPDNEC
Sbjct: 241 FAIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDARRFISQLMTVPDNEC 300

Query: 301 NSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEKAGKRKGN 360
           NSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEKAGKRKGN
Sbjct: 301 NSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEKAGKRKGN 360

Query: 361 SNKRLKGSKISHAKTWEHVDHVIMNLPASALHFLDAFRRSIPRKYWKGSLPWIHCYCFMR 420
           SNKRLKGSKISHAKTWEHVDHVIMNLPASALHFLDAFRRSIPRKYWKGSLPWIHCYCFMR
Sbjct: 361 SNKRLKGSKISHAKTWEHVDHVIMNLPASALHFLDAFRRSIPRKYWKGSLPWIHCYCFMR 420

Query: 421 ANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQQCFDEELAS 475
           ANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQQCFDEELAS
Sbjct: 421 ANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQQCFDEELAS 475

[Close]

BLAST of Csor.00g269700 vs. NCBI nr
Match: XP_023537242.1 (tRNA (guanine(37)-N1)-methyltransferase 2 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 919 bits (2376), Expect = 0.0
Identity = 460/487 (94.46%), Postives = 463/487 (95.07%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDES+FDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESEFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI
Sbjct: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120

Query: 121 VKEVMLFCDFTGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK 180
                      GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK
Sbjct: 121 -----------GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK 180

Query: 181 FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP 240
           FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP
Sbjct: 181 FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP 240

Query: 241 FAIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDARRFISQLMTVPDNEC 300
           FAIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDAR+FISQLMTVPDNEC
Sbjct: 241 FAIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDNEC 300

Query: 301 NSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEK------- 360
           NSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEK       
Sbjct: 301 NSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEKGKGIADD 360

Query: 361 -----AGKRKGNSNKRLKGSKISHAKTWEHVDHVIMNLPASALHFLDAFRRSIPRKYWKG 420
                AGKRKGNSNKRLKGS+ISHAKTWEHVDHVIMNLPASALHFLDAFRRSIPRKYWKG
Sbjct: 361 ASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALHFLDAFRRSIPRKYWKG 420

Query: 421 SLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQQC 475
           SLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQQC
Sbjct: 421 SLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQQC 476

[Close]

BLAST of Csor.00g269700 vs. NCBI nr
Match: XP_022937443.1 (tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucurbita moschata] >XP_022937444.1 tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucurbita moschata])

HSP 1 Score: 919 bits (2376), Expect = 0.0
Identity = 460/487 (94.46%), Postives = 463/487 (95.07%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI
Sbjct: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120

Query: 121 VKEVMLFCDFTGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK 180
                      GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK
Sbjct: 121 -----------GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK 180

Query: 181 FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP 240
           FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP
Sbjct: 181 FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP 240

Query: 241 FAIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDARRFISQLMTVPDNEC 300
           FAIPAAQKGCIVYANDLNPDS+RYLKINAEINKVGDRVHVHNLDAR+FISQLMTVPDNEC
Sbjct: 241 FAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDNEC 300

Query: 301 NSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEK------- 360
           NSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEK       
Sbjct: 301 NSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEKDNGIADD 360

Query: 361 -----AGKRKGNSNKRLKGSKISHAKTWEHVDHVIMNLPASALHFLDAFRRSIPRKYWKG 420
                AGKRKGNSNKRLKGS+ISHAKTWEHVDHVIMNLPASALHFLDAFR SIPRKYWKG
Sbjct: 361 ASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALHFLDAFRGSIPRKYWKG 420

Query: 421 SLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQQC 475
           SLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQQC
Sbjct: 421 SLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQQC 476

[Close]

BLAST of Csor.00g269700 vs. NCBI nr
Match: XP_023537240.1 (tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023537241.1 tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 914 bits (2363), Expect = 0.0
Identity = 460/500 (92.00%), Postives = 463/500 (92.60%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDES+FDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESEFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI
Sbjct: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120

Query: 121 VKEVMLFCDFTGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK 180
                      GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK
Sbjct: 121 -----------GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK 180

Query: 181 FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP 240
           FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP
Sbjct: 181 FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP 240

Query: 241 FAIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDARRFISQLMTVPDNEC 300
           FAIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDAR+FISQLMTVPDNEC
Sbjct: 241 FAIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDNEC 300

Query: 301 NSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEK------- 360
           NSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEK       
Sbjct: 301 NSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEKDNGIADD 360

Query: 361 ------------------AGKRKGNSNKRLKGSKISHAKTWEHVDHVIMNLPASALHFLD 420
                             AGKRKGNSNKRLKGS+ISHAKTWEHVDHVIMNLPASALHFLD
Sbjct: 361 ASICVAGKGIADDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALHFLD 420

Query: 421 AFRRSIPRKYWKGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNK 475
           AFRRSIPRKYWKGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNK
Sbjct: 421 AFRRSIPRKYWKGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNK 480

[Close]

BLAST of Csor.00g269700 vs. NCBI nr
Match: XP_022965706.1 (tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucurbita maxima] >XP_022965707.1 tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucurbita maxima])

HSP 1 Score: 909 bits (2348), Expect = 0.0
Identity = 455/487 (93.43%), Postives = 460/487 (94.46%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI
Sbjct: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120

Query: 121 VKEVMLFCDFTGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK 180
                      GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK
Sbjct: 121 -----------GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK 180

Query: 181 FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP 240
           FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP
Sbjct: 181 FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP 240

Query: 241 FAIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDARRFISQLMTVPDNEC 300
           FAIPAAQKGCIVYANDLNPDS+RYLKINAEINKVGDRVHVHNLDAR+FISQLMTVPDNEC
Sbjct: 241 FAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDNEC 300

Query: 301 NSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEK------- 360
           NSESVASVLKACEKVQVEEV GSVKTSDESVEGSRSADVSVAALKRSS+CCEK       
Sbjct: 301 NSESVASVLKACEKVQVEEVHGSVKTSDESVEGSRSADVSVAALKRSSDCCEKDNGIADD 360

Query: 361 -----AGKRKGNSNKRLKGSKISHAKTWEHVDHVIMNLPASALHFLDAFRRSIPRKYWKG 420
                AGKRKGNSNKRLKGS+ISHAKTWEHVDHVIMNLPASAL FLDAFR SIP KYWKG
Sbjct: 361 ASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAFRGSIPLKYWKG 420

Query: 421 SLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQQC 475
           SLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQQC
Sbjct: 421 SLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQQC 476

[Close]

BLAST of Csor.00g269700 vs. ExPASy TrEMBL
Match: A0A6J1FG25 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111443851 PE=3 SV=1)

HSP 1 Score: 919 bits (2376), Expect = 0.0
Identity = 460/487 (94.46%), Postives = 463/487 (95.07%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI
Sbjct: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120

Query: 121 VKEVMLFCDFTGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK 180
                      GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK
Sbjct: 121 -----------GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK 180

Query: 181 FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP 240
           FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP
Sbjct: 181 FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP 240

Query: 241 FAIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDARRFISQLMTVPDNEC 300
           FAIPAAQKGCIVYANDLNPDS+RYLKINAEINKVGDRVHVHNLDAR+FISQLMTVPDNEC
Sbjct: 241 FAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDNEC 300

Query: 301 NSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEK------- 360
           NSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEK       
Sbjct: 301 NSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEKDNGIADD 360

Query: 361 -----AGKRKGNSNKRLKGSKISHAKTWEHVDHVIMNLPASALHFLDAFRRSIPRKYWKG 420
                AGKRKGNSNKRLKGS+ISHAKTWEHVDHVIMNLPASALHFLDAFR SIPRKYWKG
Sbjct: 361 ASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALHFLDAFRGSIPRKYWKG 420

Query: 421 SLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQQC 475
           SLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQQC
Sbjct: 421 SLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQQC 476

[Close]

BLAST of Csor.00g269700 vs. ExPASy TrEMBL
Match: A0A6J1HME6 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111465526 PE=3 SV=1)

HSP 1 Score: 909 bits (2348), Expect = 0.0
Identity = 455/487 (93.43%), Postives = 460/487 (94.46%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI
Sbjct: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120

Query: 121 VKEVMLFCDFTGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK 180
                      GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK
Sbjct: 121 -----------GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK 180

Query: 181 FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP 240
           FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP
Sbjct: 181 FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP 240

Query: 241 FAIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDARRFISQLMTVPDNEC 300
           FAIPAAQKGCIVYANDLNPDS+RYLKINAEINKVGDRVHVHNLDAR+FISQLMTVPDNEC
Sbjct: 241 FAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDNEC 300

Query: 301 NSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEK------- 360
           NSESVASVLKACEKVQVEEV GSVKTSDESVEGSRSADVSVAALKRSS+CCEK       
Sbjct: 301 NSESVASVLKACEKVQVEEVHGSVKTSDESVEGSRSADVSVAALKRSSDCCEKDNGIADD 360

Query: 361 -----AGKRKGNSNKRLKGSKISHAKTWEHVDHVIMNLPASALHFLDAFRRSIPRKYWKG 420
                AGKRKGNSNKRLKGS+ISHAKTWEHVDHVIMNLPASAL FLDAFR SIP KYWKG
Sbjct: 361 ASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAFRGSIPLKYWKG 420

Query: 421 SLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQQC 475
           SLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQQC
Sbjct: 421 SLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQQC 476

[Close]

BLAST of Csor.00g269700 vs. ExPASy TrEMBL
Match: A0A6J1FGN5 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111443851 PE=3 SV=1)

HSP 1 Score: 878 bits (2268), Expect = 0.0
Identity = 440/467 (94.22%), Postives = 443/467 (94.86%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI
Sbjct: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120

Query: 121 VKEVMLFCDFTGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK 180
                      GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK
Sbjct: 121 -----------GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK 180

Query: 181 FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP 240
           FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP
Sbjct: 181 FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP 240

Query: 241 FAIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDARRFISQLMTVPDNEC 300
           FAIPAAQKGCIVYANDLNPDS+RYLKINAEINKVGDRVHVHNLDAR+FISQLMTVPDNEC
Sbjct: 241 FAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDNEC 300

Query: 301 NSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEK------- 360
           NSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEK       
Sbjct: 301 NSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEKDNGIADD 360

Query: 361 -----AGKRKGNSNKRLKGSKISHAKTWEHVDHVIMNLPASALHFLDAFRRSIPRKYWKG 420
                AGKRKGNSNKRLKGS+ISHAKTWEHVDHVIMNLPASALHFLDAFR SIPRKYWKG
Sbjct: 361 ASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALHFLDAFRGSIPRKYWKG 420

Query: 421 SLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNK 455
           SLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNK
Sbjct: 421 SLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNK 456

[Close]

BLAST of Csor.00g269700 vs. ExPASy TrEMBL
Match: A0A6J1HPS1 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111465526 PE=3 SV=1)

HSP 1 Score: 869 bits (2245), Expect = 0.0
Identity = 436/467 (93.36%), Postives = 440/467 (94.22%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI
Sbjct: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120

Query: 121 VKEVMLFCDFTGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK 180
                      GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK
Sbjct: 121 -----------GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK 180

Query: 181 FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP 240
           FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP
Sbjct: 181 FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP 240

Query: 241 FAIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDARRFISQLMTVPDNEC 300
           FAIPAAQKGCIVYANDLNPDS+RYLKINAEINKVGDRVHVHNLDAR+FISQLMTVPDNEC
Sbjct: 241 FAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDNEC 300

Query: 301 NSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEK------- 360
           NSESVASVLKACEKVQVEEV GSVKTSDESVEGSRSADVSVAALKRSS+CCEK       
Sbjct: 301 NSESVASVLKACEKVQVEEVHGSVKTSDESVEGSRSADVSVAALKRSSDCCEKDNGIADD 360

Query: 361 -----AGKRKGNSNKRLKGSKISHAKTWEHVDHVIMNLPASALHFLDAFRRSIPRKYWKG 420
                AGKRKGNSNKRLKGS+ISHAKTWEHVDHVIMNLPASAL FLDAFR SIP KYWKG
Sbjct: 361 ASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAFRGSIPLKYWKG 420

Query: 421 SLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNK 455
           SLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNK
Sbjct: 421 SLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNK 456

[Close]

BLAST of Csor.00g269700 vs. ExPASy TrEMBL
Match: A0A6J1DTR8 (tRNA (guanine(37)-N1)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111024249 PE=3 SV=1)

HSP 1 Score: 812 bits (2098), Expect = 8.63e-295
Identity = 400/484 (82.64%), Postives = 431/484 (89.05%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLKLWALRIPRELCK+AIKILNGYLIDR R+KPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKAAIKILNGYLIDRARVKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           VQ+ ELS+IPE KLEELKGLCEIELVPYSLTLGYSYWG DH+LKQILP  VEVPSSFETI
Sbjct: 61  VQAPELSQIPEAKLEELKGLCEIELVPYSLTLGYSYWGADHILKQILPPRVEVPSSFETI 120

Query: 121 VKEVMLFCDFTGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK 180
                      GH+AHLNIHDELLPYK+VIAKVIYDKNYPRIKTVVNKVG+ITN+FRVPK
Sbjct: 121 -----------GHIAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGTITNKFRVPK 180

Query: 181 FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP 240
           FE+L GE+DM TEVKQYGATFKLDY LVYWNSRLEHEH+RLVSLFQPGEIICDMFAGIGP
Sbjct: 181 FEILMGEHDMVTEVKQYGATFKLDYGLVYWNSRLEHEHVRLVSLFQPGEIICDMFAGIGP 240

Query: 241 FAIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDARRFISQLMTVPDNEC 300
           FAIPAAQK CIVY NDLNPDS++YLK+NAEINKV DRVHV+NLDAR+FISQLM VPDNE 
Sbjct: 241 FAIPAAQKECIVYGNDLNPDSVQYLKVNAEINKVSDRVHVYNLDARKFISQLMIVPDNES 300

Query: 301 NSESVASVLKACEK-------------VQVEEVQGSVKTSDESVEGSRSADVSVAALKRS 360
           NSESV SVLKAC+K             VQVEEVQ + K SDE+VE SRSAD+SV+ LKRS
Sbjct: 301 NSESVTSVLKACDKGIGGSNQESPSANVQVEEVQENSKISDETVEHSRSADISVSVLKRS 360

Query: 361 SECCEKAGKRKGNSNKRLKGSKISHAKTWEHVDHVIMNLPASALHFLDAFRRSIPRKYWK 420
           SE CEKAG++K N+NKR+KGS I HA+TWEHVDHVIMNLPASAL FLDAFR SI RKYWK
Sbjct: 361 SESCEKAGRKKRNANKRMKGSNICHARTWEHVDHVIMNLPASALQFLDAFRGSIRRKYWK 420

Query: 421 GSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQQ 471
           GSLPWIHCYCFMRANENQ+FIIS AESALN KIQDP+FHRVRDVAPNKAMYCLSFRLP+Q
Sbjct: 421 GSLPWIHCYCFMRANENQDFIISEAESALNGKIQDPVFHRVRDVAPNKAMYCLSFRLPEQ 473

[Close]

BLAST of Csor.00g269700 vs. TAIR 10
Match: AT3G56120.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 628.2 bits (1619), Expect = 5.2e-180
Identity = 314/480 (65.42%), Postives = 375/480 (78.12%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           M DESKFDV+LKLWALRIPRELCKSA +ILNGY+++ PRIKP+TEDPTC+K R VILSE 
Sbjct: 1   MFDESKFDVNLKLWALRIPRELCKSASRILNGYMLNMPRIKPITEDPTCEKTRLVILSES 60

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           V++ +LSEIPE KL +LK L E+E+VP+S+TLGYSYW  DH+LKQILP G+++PSSFETI
Sbjct: 61  VKNADLSEIPEEKLNQLKKLSELEVVPHSVTLGYSYWSADHLLKQILPDGLDIPSSFETI 120

Query: 121 VKEVMLFCDFTGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPK 180
                      GH+AHLN+HDELLP+K+VIAKVIYDKNYPRIKT+VNKVG+I+NEFRVPK
Sbjct: 121 -----------GHIAHLNLHDELLPFKDVIAKVIYDKNYPRIKTIVNKVGTISNEFRVPK 180

Query: 181 FEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGP 240
           FEVL GEN METEVKQYGA FKLDY LVYWNSRLEHEH+RL SLF+PGE +CDMFAGIGP
Sbjct: 181 FEVLAGENGMETEVKQYGARFKLDYGLVYWNSRLEHEHMRLSSLFKPGETVCDMFAGIGP 240

Query: 241 FAIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDARRFISQLMTVPDNEC 300
           FAIPAAQKGC VYANDLNPDS+RYLKINA+ NKV D + VHN+DAR+F S LM V   E 
Sbjct: 241 FAIPAAQKGCFVYANDLNPDSVRYLKINAKFNKVDDLICVHNMDARKFFSHLMAVSTCED 300

Query: 301 NSESVASVLKACEKVQVEEVQGSVKTSDESV-EGSRSADVSVAALKRSSECCEK---AGK 360
           N +SVA   K   K       G   +S E + E + S +  + A K+ S   +     GK
Sbjct: 301 NLQSVADNDKT--KEAAVSRGGETNSSGEEIRESNASINEPLGANKKPSGTTKTENGVGK 360

Query: 361 R----KGNSNKRLKGSKISHAKTWEHVDHVIMNLPASALHFLDAFRRSIPRKYWKGSLPW 420
                +G++NKRL+ + +  AK WEH+DHVIMNLPASAL FLD+F   I +KYWKG LP 
Sbjct: 361 DCKSIEGHANKRLRQTLLPIAKPWEHIDHVIMNLPASALQFLDSFSNVIQKKYWKGPLPL 420

Query: 421 IHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQQCFDEE 473
           IHCYCF+RA+E  EFII+ AE+AL   I+DP+FH+VRDVAPNKAM+CLSFRLP+ C  +E
Sbjct: 421 IHCYCFIRASETTEFIIAEAETALKFHIEDPVFHKVRDVAPNKAMFCLSFRLPEACLKQE 467

[Close]

BLAST of Csor.00g269700 vs. TAIR 10
Match: AT4G27340.1 (Met-10+ like family protein )

HSP 1 Score: 218.4 bits (555), Expect = 1.2e-56
Identity = 142/427 (33.26%), Postives = 209/427 (48.95%), Query Frame = 0


Query: 53  RYVILSEKVQSTELSEIPE------MKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQI 112
           R ++L EK    E+ ++PE       + +        ELV   LTL Y YW    +L+ +
Sbjct: 274 RLLLLDEKYSGEEVQDLPEAIKVLFAEAKMADASLSFELVKCRLTLFYDYWPMIEILEAV 333

Query: 113 LPHGVEVPSSFETIVKEVMLFCDFTGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVV 172
           LP G+ VPS+FE +           GH+AHLN+ DE LPYK +IAKV+ DKN P+I+TVV
Sbjct: 334 LPKGMIVPSAFEMV-----------GHIAHLNLRDEHLPYKRLIAKVVLDKNQPKIQTVV 393

Query: 173 NKVGSITNEFRVPKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQ 232
           NK+  I N+FR  + EVL G + + T V + G  F +D + VYWNS+L  E  RL+  F 
Sbjct: 394 NKIDPIHNDFRTMQLEVLAGNHSLVTLVVENGLRFHVDLARVYWNSKLGTERQRLLLGFD 453

Query: 233 PGEIICDMFAGIGPFAIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDAR 292
             +++CD+FAG+GP A+ AA+    VYANDLNP ++ +++ N+ +NK+  R+ + N+D R
Sbjct: 454 QNDVVCDVFAGVGPIALAAARIVKRVYANDLNPHAVEFMEQNSVVNKLEKRIEIFNMDGR 513

Query: 293 RFISQLMTVPDNECNSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKR 352
           RFI  +                                                      
Sbjct: 514 RFIKAMF----------------------------------------------------- 573

Query: 353 SSECCEKAGKRKGNSNKRLKGSKISHAKTWEHVDHVIMNLPASALHFLDAFRRSIPRKYW 412
           SSE                KG K++          V+MNLP  A   LDAFR     ++ 
Sbjct: 574 SSE----------------KGQKVT---------QVVMNLPKDAAESLDAFRGVYNDRHR 606

Query: 413 KG--SLPWIHCYCFMRANE-----NQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYC 467
               S P IH Y F +A++     ++   I+++E A++ K++     +VR VAP K M C
Sbjct: 634 DEGLSFPTIHVYGFSKASDPEFDFHERIRIALSEVAVDVKMR-----KVRLVAPGKWMLC 606

[Close]

BLAST of Csor.00g269700 vs. TAIR 10
Match: AT4G04670.1 (Met-10+ like family protein / kelch repeat-containing protein )

HSP 1 Score: 55.1 bits (131), Expect = 1.8e-07
Identity = 46/169 (27.22%), Postives = 81/169 (47.93%), Query Frame = 0


Query: 174 NEFRVPKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICD 233
           N  R    E+L G+N      ++ G  +  D +   ++     E +R+ ++    E++ D
Sbjct: 785 NGTRDSTLEILVGDNGWVNH-RENGILYSFDATKCMFSWGNLSEKLRMGNMACENEVVVD 844

Query: 234 MFAGIGPFAIP--AAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHV----HNLDARR 293
           +FAGIG F +P     K  +VYA + NP +I  L+ N E N V +R  +    + + A +
Sbjct: 845 LFAGIGYFVLPFLVRAKAKLVYACEWNPHAIEALRRNVEANSVSERCIILEGDNRITAPK 904

Query: 294 FIS---QLMTVPDNECNSESVASVLKACEKVQVEEVQGSVKTSDESVEG 334
            ++    L  +P +E +  +    L+   +  +  V G+VK SDES  G
Sbjct: 905 GVADRVNLGLIPSSEGSWVTAIQALR--PEGGILHVHGNVKDSDESSWG 950

The following BLAST results are available for this feature:

BLAST of Csor.00g269700 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F6HH45	3.9e-188	67.06	tRNA (guanine(37)-N1)-methyltransferase 2 OS=Vitis vinifera OX=29760 GN=VIT_11s0...	[more]
Q93YU6	7.3e-179	65.42	tRNA (guanine(37)-N1)-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At3...	[more]
C5XX79	8.2e-154	57.70	tRNA (guanine(37)-N1)-methyltransferase OS=Sorghum bicolor OX=4558 GN=Sb04g02539...	[more]
A9T6G5	5.0e-119	49.01	tRNA (guanine(37)-N1)-methyltransferase OS=Physcomitrium patens OX=3218 GN=PHYPA...	[more]
F4NUJ6	4.0e-76	35.32	tRNA (guanine(37)-N1)-methyltransferase OS=Batrachochytrium dendrobatidis (strai...	[more]

BLAST of Csor.00g269700 vs. NCBI nr


Match Name	Identity	Description
KAG6586416.1	100.00	tRNA (guanine(37)-N1)-methyltransferase 2, partial [Cucurbita argyrosperma subsp...	[more]
XP_023537242.1	94.46	tRNA (guanine(37)-N1)-methyltransferase 2 isoform X2 [Cucurbita pepo subsp. pepo...	[more]
XP_022937443.1	94.46	tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucurbita moschata] >XP_02...	[more]
XP_023537240.1	92.00	tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucurbita pepo subsp. pepo...	[more]
XP_022965706.1	93.43	tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucurbita maxima] >XP_0229...	[more]

BLAST of Csor.00g269700 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FG25	0.0	94.46	tRNA (guanine(37)-N1)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1HME6	0.0	93.43	tRNA (guanine(37)-N1)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111465...	[more]
A0A6J1FGN5	0.0	94.22	tRNA (guanine(37)-N1)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1HPS1	0.0	93.36	tRNA (guanine(37)-N1)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111465...	[more]
A0A6J1DTR8	8.63e-295	82.64	tRNA (guanine(37)-N1)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111...	[more]

BLAST of Csor.00g269700 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G56120.1	5.2e-180	65.42	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT4G27340.1	1.2e-56	33.26	Met-10+ like family protein	[more]
AT4G04670.1	1.8e-07	27.22	Met-10+ like family protein / kelch repeat-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 190..464 e-value: 1.2E-58 score: 199.7
None	No IPR available	GENE3D	3.30.300.110		coord: 101..188 e-value: 1.5E-19 score: 71.8
None	No IPR available	PANTHER	PTHR23245	TRNA METHYLTRANSFERASE	coord: 2..469
None	No IPR available	PANTHER	PTHR23245:SF36	TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE	coord: 2..469
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 231..288 e-value: 2.125E-5 score: 41.6467
IPR030382	SAM-dependent methyltransferase TRM5/TYW2-type	PFAM	PF02475	Met_10	coord: 130..403 e-value: 2.2E-63 score: 213.6
IPR030382	SAM-dependent methyltransferase TRM5/TYW2-type	PROSITE	PS51684	SAM_MT_TRM5_TYW2	coord: 128..466 score: 100.607193
IPR025792	tRNA (guanine(37)-N(1))-methyltransferase, eukaryotic	HAMAP	MF_03152	TRM5	coord: 2..466 score: 25.325558
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 128..466

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g269700.m01	Csor.00g269700.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030488	tRNA methylation
cellular_component	GO:0005759	mitochondrial matrix
cellular_component	GO:0005634	nucleus
molecular_function	GO:0052906	tRNA (guanine(37)-N(1))-methyltransferase activity
molecular_function	GO:0009019	tRNA (guanine-N1-)-methyltransferase activity