Csor.00g269220 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g269220
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionvacuolar protein sorting-associated protein 32 homolog 2
LocationCsor_Chr12: 16078516 .. 16081627 (+)
RNA-Seq ExpressionCsor.00g269220
SyntenyCsor.00g269220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTAAACAGGCTTTTTGGTAAACCTAAACAAGAAGCCAATGCTTTGGCTACGTTAGACAAGTTGAATGAGGTATGTTTTACTTCGATGTTTTATTGTTTTTGATATTCTTTGTATGGAAATGATCTTTTCCTTTTCTCACTTGTTCTGAACCTATTTCATCGATCTTGATGGATGCTTGGCTTCTCGATCATTCCGTTTTTTTCCTCGTCTTTTCCAAAAATTAGTGTATATGACGGCGTAGTAATTGGTTCTACTTGACGGAGTATTTGTGTTTATGACGTCGTATTAACTTGTTCTCATTGATAGAGTACTAGTGTTTATGACGTCGTAGTAATTGGTTCTCCTTGACCGAGTATTAATGTTTATAATGTGGTAGTTATTGGTTTCCAGTTGAAATTATCGTCTTATTTGGATTTTTTAACTCGTAATTTTCCAGTTGAAATGTCGTAGTTACTGCCGTTCTTTAGGTGAAATTTCGCTTATGTAAGAAAGGAAACCTGTGTACTACAATGCTGTCGTTCTTTGGAATTGCGTCTAAAATTGTGGTTGCGTAGATTTTTGTAGTACGACTAAGCTTTTCATAATTGATACCGATATTTTTGTGCACTTCATTGTCCGGCGTTGTTTGATTTAATTGCTTCCTTTTCATCGTCTGGGATTATCTGTTTAGACACTTGAAATGCTGGAGAAGAAAGAGAACGTTCTTATTAAGAAGGCTGCTGCAGAGGTTGAAAAAGCTAAAGAATTCACAAGAGTGAAGAATAAACGAGGTATGTTATGTAATTGGGTGATCTCTTCTATGGTCAAATGCTTCTTGGTATTAATTTTTATTCCTTTTTTGAGTATTTGTTCACATGTGAAGTGTGAATAAGATTTAATTAACACCCGGTTTCTCCGCTCTGGAGTGTTAATTTGATGGTACTGCCATTCTATGGTCAAATGCAAGTTGTGCTTATGTCTGTATATTGCAATGTGATACTCCGCAGAATTTATTACTGAATCCTCCATCTACTCTTTTTTGTTCATTTCCTTATTAGGAAAATCTTGTGCTTGATGAATTATTCTACACTCTACATCCTCAATTGATGAAGTAGCCTCGTGCATTTTTTGGAGGATGATTTTTCTTGTGTTTTGTTGCCAGTCTTTGTGACTGATGGTTGCAATGTATCCCAATATTTTTTGTTGTAGTTTATGATGGCCATACATTTGTAGCTTTGTTTATTTCAAATCTTCGTTTGAGCTTTCTTTCATTTTTGGAGTTTTGTGATTTTCCTTGTTCTTATCTTTATCAGTGTTTTGGATATCACTTTCAAATGGGTTTTAATTTTATATTTCATGTCTATTTTAGTCTGAGCTGCATTTTTGGAGTTTATTCTGTCTGCTTTTTTGGGCTCTATAGTTTGTTCCTTTTCATTCTTCTTGATTTATTTAATATTTTTAAAATCATTGTAATTTTTAAAATCCTTCTATCTCTATATTACCTCGTGTTCTTATTTGCCAACCCATTTGGATCGTTTTGTTTATTGGATATGAATATCAAATATTAGGATTAAATGTCTAGGATATGAAATAGTTCTGTTTATAACTCGAACATTTGTTCTTTACAAGAATTGTTGTTTATAGGTTTCCTTCCTGACAATTTAATTAGTTTAATCAATTAGTTAGATCTGAAAGAAAAAATTAATGATATTAAATTGAAAGATTCGATTCATAGTCATTAGAATTGGTTGCAGCGGCCATACAATGTTTGAAGAGGAAAAGGCTTTATGAACAGCAAGTAGAGCAACTTGGAAATTTCCAATTGCGTATCCATGATCAGGTGAGCTGGAGTCGTTATGTACTGATTAATGAGACATTTATTTCTTGTACTTTCAGTTAACTGTATTGAAATCAACCTTTTACTTTATTAACAATTTTTTTTTTAACTTTTGCAGATGATTATGTTAGAAGGCGCAAAAGCTACAACGGAGACTGTCGATGCTTTGAGAACAGGAGCATCTGCAATGAAGGCCATGCAGAAAGCAACGTATGCCTAATATTTATTTGATTTGCTTTCTTTCTGCCTAACATTCTTTTTGTCTTCACAATCGATCTTTTAGCACAACTATTAACATTACTATTGCCTAAGCCGGAAAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTGTACAAAATATTGCTCAACTTATTTATGCACTTCGTTCATCTAGTTATTGATGTTAAGCTAACCAATCTTGAGAAAGTTAGCCATTACCATTAAGCATTACTTGCAATGGCAACTGAAAAATGAAATAATTTGTGAAAGTTTGTTTCATGTTTTATTTCTTTAACCGAGGTTGTGAAATTTGGAACCGTACTTATTTTAAAAATGTAGGCTGATTATTTGAAACTTTCTTCTCCAGAAACATTGATGATGTGGACAAGACGATGGATGAGATCAATGAGCAGACTGAGAATATGAAACAGATACAGGAAGCGCTGTCAACTCCAATTGGTGCTGCAGCTGATTTCGATGAGGTACTTAATCTTTGGCTTGTTTCTTGGCCTTTCCTGGTATTTGCTTATGTCTCGCAATTCATCTATTAACAATGGTGGTGTAATCAGGATGAATTGGAGGCAGAACTCGAAGAGCTTGAAAGTGCTGAGTTGGAAGAACAACTTCTCCAGCCAGCCTCAACGGCTCCTGCTGGACCTGTCTCAGTCCTGTCAGGCCGAGTTCCTGCTCGCCCTGTTCCACAGAAGAGAACTGCGGAAGAAGACGAGCTGGCAGCTCTGCAAGCTGAGATGGCCCTCTAACCAGGTTTGTTCCGTAGCCCAATCTATAAGCTGAGTGAGCTCCGATTAACTTTCTATAATCACACTGTTCAAGTCCAAGTACTCTAAATTTGTAGTTGAACGTTGTTTTATCTTAAAAGATTACCTGTTATGGAGCAGAGAACTGAATTTTAATTGGACATTGCAGGTGGTGCATACGAGTATCATCTTCTGGCAGATTGCATCCGAATCTTCTAAGCGTTTGATTGAAATTTTACACAAATTACCCTTCCCTGCGGCAAGACAATGTATATATAAAGGTGCAAAATTACTGGGATCTTCATCTTAA

mRNA sequence

ATGTTAAACAGGCTTTTTGGTAAACCTAAACAAGAAGCCAATGCTTTGGCTACGTTAGACAAGTTGAATGAGACACTTGAAATGCTGGAGAAGAAAGAGAACGTTCTTATTAAGAAGGCTGCTGCAGAGGTTGAAAAAGCTAAAGAATTCACAAGAGTGAAGAATAAACGAGCGGCCATACAATGTTTGAAGAGGAAAAGGCTTTATGAACAGCAAGTAGAGCAACTTGGAAATTTCCAATTGCGTATCCATGATCAGATGATTATGTTAGAAGGCGCAAAAGCTACAACGGAGACTGTCGATGCTTTGAGAACAGGAGCATCTGCAATGAAGGCCATGCAGAAAGCAACAAACATTGATGATGTGGACAAGACGATGGATGAGATCAATGAGCAGACTGAGAATATGAAACAGATACAGGAAGCGCTGTCAACTCCAATTGGTGCTGCAGCTGATTTCGATGAGGATGAATTGGAGGCAGAACTCGAAGAGCTTGAAAGTGCTGAAAGAGAACTGCGGAAGAAGACGAGCTGGCAGCTCTGCAAGCTGAGATGGCCCTCTAACCAGGTGGTGCATACGAGTATCATCTTCTGGCAGATTGCATCCGAATCTTCTAAGCGTTTGATTGAAATTTTACACAAATTACCCTTCCCTGCGGCAAGACAATGTATATATAAAGGTGCAAAATTACTGGGATCTTCATCTTAA

Coding sequence (CDS)

ATGTTAAACAGGCTTTTTGGTAAACCTAAACAAGAAGCCAATGCTTTGGCTACGTTAGACAAGTTGAATGAGACACTTGAAATGCTGGAGAAGAAAGAGAACGTTCTTATTAAGAAGGCTGCTGCAGAGGTTGAAAAAGCTAAAGAATTCACAAGAGTGAAGAATAAACGAGCGGCCATACAATGTTTGAAGAGGAAAAGGCTTTATGAACAGCAAGTAGAGCAACTTGGAAATTTCCAATTGCGTATCCATGATCAGATGATTATGTTAGAAGGCGCAAAAGCTACAACGGAGACTGTCGATGCTTTGAGAACAGGAGCATCTGCAATGAAGGCCATGCAGAAAGCAACAAACATTGATGATGTGGACAAGACGATGGATGAGATCAATGAGCAGACTGAGAATATGAAACAGATACAGGAAGCGCTGTCAACTCCAATTGGTGCTGCAGCTGATTTCGATGAGGATGAATTGGAGGCAGAACTCGAAGAGCTTGAAAGTGCTGAAAGAGAACTGCGGAAGAAGACGAGCTGGCAGCTCTGCAAGCTGAGATGGCCCTCTAACCAGGTGGTGCATACGAGTATCATCTTCTGGCAGATTGCATCCGAATCTTCTAAGCGTTTGATTGAAATTTTACACAAATTACCCTTCCCTGCGGCAAGACAATGTATATATAAAGGTGCAAAATTACTGGGATCTTCATCTTAA

Protein sequence

MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAERELRKKTSWQLCKLRWPSNQVVHTSIIFWQIASESSKRLIEILHKLPFPAARQCIYKGAKLLGSSS
Homology
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BLAST of Csor.00g269220 vs. ExPASy Swiss-Prot
Match: Q9SZE4 (Vacuolar protein sorting-associated protein 32 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=VPS32.2 PE=1 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 7.5e-76
Identity = 155/171 (90.64%), Postives = 163/171 (95.32%), Query Frame = 0 

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M+NRLFGKPKQEANAL TLDKLNETLEMLEKKE VL+KKA AEVEKAKE++R KNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYE QVEQLGNFQLRIHDQMIMLEGAKATTETVDALR+GASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAERE 172
           DVDKTMDEINEQTENMKQIQEAL+TP+GAAADFDEDEL AEL+ELES E E
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELE 171

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BLAST of Csor.00g269220 vs. ExPASy Swiss-Prot
Match: O82197 (Vacuolar protein sorting-associated protein 32 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=VPS32.1 PE=1 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 1.6e-73
Identity = 153/170 (90.00%), Postives = 158/170 (92.94%), Query Frame = 0 

Query: 2   LNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQ 61
           +NRLFGKPKQE + L TLDKLNETLEMLEKKENVL+KKA  EVEKAKEF+R KNKRAAIQ
Sbjct: 3   MNRLFGKPKQETSTLQTLDKLNETLEMLEKKENVLLKKATGEVEKAKEFSRAKNKRAAIQ 62

Query: 62  CLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDD 121
           CLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDD
Sbjct: 63  CLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDD 122

Query: 122 VDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAERE 172
           VDKTMDEINEQTENMKQIQEALS P G A DFDEDELEAEL+ELE AE E
Sbjct: 123 VDKTMDEINEQTENMKQIQEALSAPFG-ANDFDEDELEAELDELEGAELE 171

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BLAST of Csor.00g269220 vs. ExPASy Swiss-Prot
Match: Q7ZVC4 (Charged multivesicular body protein 4b OS=Danio rerio OX=7955 GN=chmp4b PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.8e-21
Identity = 72/163 (44.17%), Postives = 95/163 (58.28%), Query Frame = 0 

Query: 7   GKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQCLKRK 66
           GK  + A+    + +L ET EML KK+  L KK   E+  AK+    KNKRAA+Q LKRK
Sbjct: 12  GKGGKSASPQEAIQRLRETEEMLTKKQEFLEKKIEQELVTAKK-NGTKNKRAALQALKRK 71

Query: 67  RLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTM 126
           + YE+Q+ Q+      I  Q   LE A   TE +  +   A AMKA     +ID VD+ M
Sbjct: 72  KRYEKQLAQIDGTLSTIEFQREALENAHTNTEVIKNMGYAAKAMKAAHDNMDIDKVDELM 131

Query: 127 DEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAE 170
            +I EQ E  ++I +A+S P+G   +FDEDEL AELEELE  E
Sbjct: 132 QDIIEQQELAQEISDAISKPVGFGEEFDEDELLAELEELEQEE 173

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BLAST of Csor.00g269220 vs. ExPASy Swiss-Prot
Match: Q6IQ73 (Charged multivesicular body protein 4c OS=Danio rerio OX=7955 GN=chmp4c PE=2 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 5.3e-21
Identity = 72/163 (44.17%), Postives = 94/163 (57.67%), Query Frame = 0 

Query: 7   GKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQCLKRK 66
           GK  +       + KL ET EML KK++ L KK  AE+  AK+    KNKRAA+Q LKRK
Sbjct: 12  GKGGKGPTPQEAIQKLRETEEMLAKKQDFLEKKIDAELLIAKK-NGTKNKRAALQALKRK 71

Query: 67  RLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTM 126
           + YE+Q+ Q+      I  Q   LE A   TE +  +   A AMK   +  +ID VD  M
Sbjct: 72  KRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGFAAKAMKTAHENMDIDKVDDLM 131

Query: 127 DEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAE 170
            +I EQ E  ++I +A+S P+G   +FDEDEL AELEELE  E
Sbjct: 132 QDITEQQELAQEISDAISRPVGFGEEFDEDELMAELEELEQEE 173

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BLAST of Csor.00g269220 vs. ExPASy Swiss-Prot
Match: Q5XGW6 (Charged multivesicular body protein 4b OS=Xenopus laevis OX=8355 GN=chmp4b PE=2 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.2e-20
Identity = 72/172 (41.86%), Postives = 99/172 (57.56%), Query Frame = 0 

Query: 1   MLNRLF---GKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKR 60
           ++ +LF   GK  +  +    + KL +T EML KK+  L KK   E+  AK+    KNKR
Sbjct: 3   LIGKLFGTGGKGAKGPSPQEAIQKLRDTEEMLAKKQEFLEKKIEQELVTAKKH-GTKNKR 62

Query: 61  AAIQCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKAT 120
           AA+Q LKRK+ YE+Q+ Q+      I  Q   LE A   TE +  +   A AMKA     
Sbjct: 63  AALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGFAAKAMKAAHDNM 122

Query: 121 NIDDVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAE 170
           +I+ VD+ M +I +Q E  ++I +A+S P+G   DFDEDEL AELEELE  E
Sbjct: 123 DIEKVDELMQDIADQQELAQEISDAISKPVGFGEDFDEDELMAELEELEQEE 173

[Close]
BLAST of Csor.00g269220 vs. NCBI nr
Match: KAG6586464.1 (Vacuolar protein sorting-associated protein 32-like 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 439 bits (1129), Expect = 7.54e-155
Identity = 235/235 (100.00%), Postives = 235/235 (100.00%), Query Frame = 0 

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI
Sbjct: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAERELRKKTSWQL 180
           DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAERELRKKTSWQL
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAERELRKKTSWQL 180

Query: 181 CKLRWPSNQVVHTSIIFWQIASESSKRLIEILHKLPFPAARQCIYKGAKLLGSSS 235
           CKLRWPSNQVVHTSIIFWQIASESSKRLIEILHKLPFPAARQCIYKGAKLLGSSS
Sbjct: 181 CKLRWPSNQVVHTSIIFWQIASESSKRLIEILHKLPFPAARQCIYKGAKLLGSSS 235

[Close]
BLAST of Csor.00g269220 vs. NCBI nr
Match: KAG6599497.1 (Vacuolar protein sorting-associated protein 32-like 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 333 bits (853), Expect = 3.87e-113
Identity = 186/219 (84.93%), Postives = 196/219 (89.50%), Query Frame = 0 

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANALATL+KL+ETLEMLEKKENVL+KKA+AEVEKAKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAERELRKKTSWQL 180
           DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAEREL KK + QL
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAERELLKKMNSQL 180

Query: 181 CKLRWPSNQVVHTSIIFWQIASESSKRLIEILHKLPFPA 219
           CK RWPSNQ+V     +         R  +I ++L FPA
Sbjct: 181 CKRRWPSNQIVPYKEAY-------DGRSFKIAYELFFPA 212

[Close]
BLAST of Csor.00g269220 vs. NCBI nr
Match: XP_022937864.1 (vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita moschata] >XP_022937865.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita moschata] >XP_022937866.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita moschata] >XP_022937867.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita moschata] >XP_023536810.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita pepo subsp. pepo] >XP_023536811.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita pepo subsp. pepo] >XP_023536812.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita pepo subsp. pepo] >KAG7021320.1 Vacuolar protein sorting-associated protein 32-like 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 307 bits (787), Expect = 5.11e-103
Identity = 170/171 (99.42%), Postives = 170/171 (99.42%), Query Frame = 0 

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI
Sbjct: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAERE 171
           DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAE E
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELE 171

[Close]
BLAST of Csor.00g269220 vs. NCBI nr
Match: XP_022965914.1 (vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita maxima] >XP_022965915.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita maxima] >XP_022965916.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita maxima] >XP_022965917.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita maxima])

HSP 1 Score: 307 bits (787), Expect = 5.11e-103
Identity = 170/171 (99.42%), Postives = 170/171 (99.42%), Query Frame = 0 

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI
Sbjct: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAERE 171
           DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAE E
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELE 171

[Close]
BLAST of Csor.00g269220 vs. NCBI nr
Match: KAG7030473.1 (Vacuolar protein sorting-associated protein 32-like 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 301 bits (770), Expect = 1.97e-100
Identity = 165/171 (96.49%), Postives = 169/171 (98.83%), Query Frame = 0 

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANALATL+KL+ETLEMLEKKENVL+KKA+AEVEKAKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAERE 171
           DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAE E
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELE 171

[Close]
BLAST of Csor.00g269220 vs. ExPASy TrEMBL
Match: A0A6J1HQ32 (vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucurbita maxima OX=3661 GN=LOC111465657 PE=4 SV=1)

HSP 1 Score: 307 bits (787), Expect = 2.47e-103
Identity = 170/171 (99.42%), Postives = 170/171 (99.42%), Query Frame = 0 

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI
Sbjct: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAERE 171
           DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAE E
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELE 171

[Close]
BLAST of Csor.00g269220 vs. ExPASy TrEMBL
Match: A0A6J1FBK1 (vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucurbita moschata OX=3662 GN=LOC111444128 PE=4 SV=1)

HSP 1 Score: 307 bits (787), Expect = 2.47e-103
Identity = 170/171 (99.42%), Postives = 170/171 (99.42%), Query Frame = 0 

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI
Sbjct: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAERE 171
           DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAE E
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELE 171

[Close]
BLAST of Csor.00g269220 vs. ExPASy TrEMBL
Match: A0A6J1G2S3 (vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita moschata OX=3662 GN=LOC111450291 PE=4 SV=1)

HSP 1 Score: 300 bits (767), Expect = 2.73e-100
Identity = 164/171 (95.91%), Postives = 169/171 (98.83%), Query Frame = 0 

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANALATL+KL+ETLEMLEKKENVL+KKA+AEVEKAKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAERE 171
           DVDKTMD+INEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAE E
Sbjct: 121 DVDKTMDDINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELE 171

[Close]
BLAST of Csor.00g269220 vs. ExPASy TrEMBL
Match: A0A6J1DML3 (vacuolar protein sorting-associated protein 32 homolog 2-like OS=Momordica charantia OX=3673 GN=LOC111021942 PE=4 SV=1)

HSP 1 Score: 300 bits (767), Expect = 2.82e-100
Identity = 164/171 (95.91%), Postives = 169/171 (98.83%), Query Frame = 0 

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           MLNRLFGKPKQEA+ALATLDKL+ETLEMLEKKENVL+KKAAAEVE+AKEFTRVKNKRAAI
Sbjct: 1   MLNRLFGKPKQEASALATLDKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAERE 171
           DVDKTMDEINEQTENMKQIQEALS PIGAAADFDEDELEAELEELESAE E
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEELESAELE 171

[Close]
BLAST of Csor.00g269220 vs. ExPASy TrEMBL
Match: A0A6J1KB41 (vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita maxima OX=3661 GN=LOC111493861 PE=4 SV=1)

HSP 1 Score: 299 bits (765), Expect = 5.49e-100
Identity = 164/171 (95.91%), Postives = 168/171 (98.25%), Query Frame = 0 

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANALATL+KL+ETLEMLEKKENVL+KKA+AEVEKAKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAERE 171
           DVDKTMDEINEQTENMKQIQEALS PIGAAADFDEDELEAELEELESAE E
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELESAELE 171

[Close]
BLAST of Csor.00g269220 vs. TAIR 10
Match: AT4G29160.1 (SNF7 family protein )

HSP 1 Score: 285.0 bits (728), Expect = 5.3e-77
Identity = 155/171 (90.64%), Postives = 163/171 (95.32%), Query Frame = 0 

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M+NRLFGKPKQEANAL TLDKLNETLEMLEKKE VL+KKA AEVEKAKE++R KNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYE QVEQLGNFQLRIHDQMIMLEGAKATTETVDALR+GASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAERE 172
           DVDKTMDEINEQTENMKQIQEAL+TP+GAAADFDEDEL AEL+ELES E E
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELE 171

[Close]
BLAST of Csor.00g269220 vs. TAIR 10
Match: AT4G29160.3 (SNF7 family protein )

HSP 1 Score: 285.0 bits (728), Expect = 5.3e-77
Identity = 155/171 (90.64%), Postives = 163/171 (95.32%), Query Frame = 0 

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M+NRLFGKPKQEANAL TLDKLNETLEMLEKKE VL+KKA AEVEKAKE++R KNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYE QVEQLGNFQLRIHDQMIMLEGAKATTETVDALR+GASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAERE 172
           DVDKTMDEINEQTENMKQIQEAL+TP+GAAADFDEDEL AEL+ELES E E
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELE 171

[Close]
BLAST of Csor.00g269220 vs. TAIR 10
Match: AT2G19830.1 (SNF7 family protein )

HSP 1 Score: 277.3 bits (708), Expect = 1.1e-74
Identity = 153/170 (90.00%), Postives = 158/170 (92.94%), Query Frame = 0 

Query: 2   LNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQ 61
           +NRLFGKPKQE + L TLDKLNETLEMLEKKENVL+KKA  EVEKAKEF+R KNKRAAIQ
Sbjct: 3   MNRLFGKPKQETSTLQTLDKLNETLEMLEKKENVLLKKATGEVEKAKEFSRAKNKRAAIQ 62

Query: 62  CLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDD 121
           CLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDD
Sbjct: 63  CLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDD 122

Query: 122 VDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAERE 172
           VDKTMDEINEQTENMKQIQEALS P G A DFDEDELEAEL+ELE AE E
Sbjct: 123 VDKTMDEINEQTENMKQIQEALSAPFG-ANDFDEDELEAELDELEGAELE 171

[Close]
BLAST of Csor.00g269220 vs. TAIR 10
Match: AT4G29160.2 (SNF7 family protein )

HSP 1 Score: 236.9 bits (603), Expect = 1.7e-62
Identity = 130/144 (90.28%), Postives = 137/144 (95.14%), Query Frame = 0 

Query: 28  MLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQCLKRKRLYEQQVEQLGNFQLRIHDQM 87
           MLEKKE VL+KKA AEVEKAKE++R KNKRAAIQCLKRKRLYE QVEQLGNFQLRIHDQM
Sbjct: 1   MLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAIQCLKRKRLYEGQVEQLGNFQLRIHDQM 60

Query: 88  IMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALSTPI 147
           IMLEGAKATTETVDALR+GASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEAL+TP+
Sbjct: 61  IMLEGAKATTETVDALRSGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALATPM 120

Query: 148 GAAADFDEDELEAELEELESAERE 172
           GAAADFDEDEL AEL+ELES E E
Sbjct: 121 GAAADFDEDELAAELDELESEELE 144

[Close]
BLAST of Csor.00g269220 vs. TAIR 10
Match: AT5G09260.1 (vacuolar protein sorting-associated protein 20.2 )

HSP 1 Score: 57.4 bits (137), Expect = 1.8e-08
Identity = 49/167 (29.34%), Postives = 81/167 (48.50%), Query Frame = 0 

Query: 2   LNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQ 61
           +  LF K  +       +  L      L + +  L K   AE + A++  R K K  A+ 
Sbjct: 1   MGNLFVKKPKITEVDRAILSLKTQRRKLGQYQQQLEKVIEAEKQAARDLIREKRKDRALL 60

Query: 62  CLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDD 121
            LK+KR  E+ ++Q+  + + +  Q+  +E         ++L+ G +A+KA+Q   N+DD
Sbjct: 61  ALKKKRTQEELLKQVDQWLINVEQQLADIELTSKQKAVFESLKQGNNAIKAIQSEVNLDD 120

Query: 122 VDKTMDEINEQTENMKQIQEALSTPIGAAADF-DEDELEAELEELES 168
           V K MD+    T   K  Q+ LS  +G      DE+E+ AE + LES
Sbjct: 121 VQKLMDD----TAEAKAYQDELSAILGEKLSAEDEEEILAEFDNLES 163

The following BLAST results are available for this feature:
BLAST of Csor.00g269220 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9SZE47.5e-7690.64Vacuolar protein sorting-associated protein 32 homolog 2 OS=Arabidopsis thaliana... [more]
O821971.6e-7390.00Vacuolar protein sorting-associated protein 32 homolog 1 OS=Arabidopsis thaliana... [more]
Q7ZVC41.8e-2144.17Charged multivesicular body protein 4b OS=Danio rerio OX=7955 GN=chmp4b PE=2 SV=... [more]
Q6IQ735.3e-2144.17Charged multivesicular body protein 4c OS=Danio rerio OX=7955 GN=chmp4c PE=2 SV=... [more]
Q5XGW61.2e-2041.86Charged multivesicular body protein 4b OS=Xenopus laevis OX=8355 GN=chmp4b PE=2 ... [more]
BLAST of Csor.00g269220 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG6586464.17.54e-155100.00Vacuolar protein sorting-associated protein 32-like 2, partial [Cucurbita argyro... [more]
KAG6599497.13.87e-11384.93Vacuolar protein sorting-associated protein 32-like 2, partial [Cucurbita argyro... [more]
XP_022937864.15.11e-10399.42vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita moschata] >X... [more]
XP_022965914.15.11e-10399.42vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita maxima] >XP_... [more]
KAG7030473.11.97e-10096.49Vacuolar protein sorting-associated protein 32-like 2 [Cucurbita argyrosperma su... [more]
BLAST of Csor.00g269220 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1HQ322.47e-10399.42vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucurbita maxima OX=... [more]
A0A6J1FBK12.47e-10399.42vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucurbita moschata O... [more]
A0A6J1G2S32.73e-10095.91vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita mosch... [more]
A0A6J1DML32.82e-10095.91vacuolar protein sorting-associated protein 32 homolog 2-like OS=Momordica chara... [more]
A0A6J1KB415.49e-10095.91vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita maxim... [more]
BLAST of Csor.00g269220 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G29160.15.3e-7790.64SNF7 family protein [more]
AT4G29160.35.3e-7790.64SNF7 family protein [more]
AT2G19830.11.1e-7490.00SNF7 family protein [more]
AT4G29160.21.7e-6290.28SNF7 family protein [more]
AT5G09260.11.8e-0829.34vacuolar protein sorting-associated protein 20.2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 151..175
NoneNo IPR availableCOILSCoilCoilcoord: 122..142
NoneNo IPR availableCOILSCoilCoilcoord: 12..39
NoneNo IPR availableGENE3D6.10.250.1710coord: 117..170
e-value: 1.2E-9
score: 39.9
NoneNo IPR availableGENE3D1.10.287.1060coord: 14..91
e-value: 2.8E-22
score: 80.6
NoneNo IPR availablePANTHERPTHR22761:SF71SNF7 FAMILY-RELATEDcoord: 1..172
NoneNo IPR availablePANTHERPTHR22761CHARGED MULTIVESICULAR BODY PROTEINcoord: 1..172
IPR005024Snf7 familyPFAMPF03357Snf7coord: 20..170
e-value: 5.6E-43
score: 146.5

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g269220.m01Csor.00g269220.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway
biological_process GO:0006900 vesicle budding from membrane
biological_process GO:0007034 vacuolar transport
cellular_component GO:0009898 cytoplasmic side of plasma membrane
cellular_component GO:0000815 ESCRT III complex
cellular_component GO:0005771 multivesicular body