Csor.00g267470 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g267470
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionDNA replication licensing factor MCM2
LocationCsor_Chr19: 4429445 .. 4431773 (+)
RNA-Seq ExpressionCsor.00g267470
SyntenyCsor.00g267470
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGGGCCAACTCCCGATGGAAACTCCGGCAATCCGCCGTCGGAGCTCGATTCCCCCACCACCTCTGCTGGTTTCGAAACCGACCAGCTTCCTCATCTCAGCCACATTTCCGACAACTACCCCGACGAGGACGAAGCTGCCGTTGATCCTCACATCGTTCACGACGAGCCTGATCCCGATGAAGAAGAGGAAGGAGAGGATCTCTACCACGACAATTTCCTGGAGTCGGTTCTCGAAAATCAAATCCCGTTTTGTTTTCTTGTTGTGTTTTTTAATAAAAAAATCTCGAAATCTCTCGCCTAATGTAGTACCGATCTAATGAATTTTGCTCTTAACCTTGTAGCGATTATCGGAGGATGGACGACCATGACCAGTACGAGTCCTTGGGCCTCGACGATTCTTTAGAGGACGAGAGGGATTTGGATCAGATTATGGAGGGACAGGCGAGCTGCCGAGATAGAACTGGACACTCGCGATGCTCAGTTCACCCGTCGGGAAGATTCCTGAGCTATTACATGATCAAGGTAATTTTTTTGACTATTTATATTCTATATGAACAGCAAATTGTAACCTTTTCCCGTCGATTTTCAGCGTGTTCACTCGCTCAGCAAGTTGTCAATACATTACTGAGCTATTCGTGCTTCCATTCTTTTCTCTACCTTTCGTTCGTGTTTTCTTTAATGTATTACTATTCTAATTTTAATTTTAGGATTGATCATGCTTGAGAATATGAATTATGCACACATATTGATTCTTTCCAGCTTTACTTTTCCCTAACAACAAAAGCTTGAGATATGTAGGAACTAATTGAAGTGAAACTCACTCTAACTGATGAGTTTCAATATCCTGAAAATCTTTAGTTTCAATTTGTATGAAGTTATTTTCAGGCACAAGTTTCCAAAGTTTATACAACCATAGAAGATTGACCTGAAAACTTTCGCAACAAAAAGAATAGTGAAGCATTCTGTTCTGGTTTCAAGCCATTCTGACTTGTCATCCAGAACATTAGTCATCTGCTCTAACCAATTACAAAAATTCCTTTGTTGGTATACGCCGAATATGTTTTTATATATGGTTTTTATAAATTAGTTGAAGATACCTTGGGGGTCTAACCTCCTAAAAGGAACTGAAAAGCTTAGAGACGTTTTCCTTGATGTTGCTTTTACTACTCTTAGCAGCTGGAAGTTATTAGTTGTTTCCCTTTTGATTTATTTTTTTGGAGACTGGCAGATTTAACTGGTCTTGAATTTAACTAGCTAATTTGTATGTTTTGTTTCAGACTCGGAGGATGACAATTACAGGGCCTTCAAAAAGAGCAAGAGCTGATTTTCGGCCTCCAGCTGCTGCAAGAAGCTATGATGACATTGATGGTATGCAAGAGTTCTCCTGGTAGATCACAAAGGGAAACTTCACGAGACGACGTTGCCCATTACAGATCATTCTGTTGATGACCAATATGAGGTATTTAAGGCTGTCACATCTCCTTTATTTTCCTCATATTAGGATTTTGCACCTAATTATTGTTAGCCATATTTTGTAACTATAACAATTTCCCTCTAATTTAGGATGAAGACGATGATGAAAATGAGAATGAGATGTATCGTGTTCAAGGGCCACTTAGAGAACATGTTACCATGGATGCGGTTCGGCGCTGTTATGTGAAAAAGTTCAAAAAATTCTTGGAAACATATGTGAATCCAAAGAGTGGAAATGGTGAGCTTGAGTACATGCGACTGATAAATGAGATGGTGTTAGGTGAGCATCTGCTTCACTGTACTTGCAGTGGATTGTTAACATTTAGTTTATTTTGTAAAATGATAAAACGAATATTGTTTTCTAAGATAACCTGTTAGAGTCATGTTCATGCTTCTCTTATTTTTCTGACTGGTTGCTTTAATGTTTGATTGCAGCCAATAGGTGCAGTTTGGAGATTGATTACAAACAGTTTATCTTTGTCCATCCGAATATTGCAATCTGGTTGGCTGATGCACCCCAGCCCGTCTTAGAAGTTATGGAAGATGTTGCAAAGAAGGTTGTCTTTGACATACATCCAAATTACAAAAATATCCACCAAAAGATCTATGTTCGCATCAATAATTTACCAGTCTATGATCAGATTCGAAATATTAGGTATCCTTGTAGATTCTCATGGTGCAGGGCCTTTTCAGATATATGTTTTCTTTTATTATTTTCCTAGTCATTCCATTTTCAATTGTGTCCCGTTGCACTTTGTAGGCAAATTCATTTAAATACCATGATTCGGAATTGGGGCGTAGTTAAACCCGACGCTCTGGGTCTTCCCCCAGTTGCAGCAGGTGAAGTATGA

mRNA sequence

ATGGCTGGGCCAACTCCCGATGGAAACTCCGGCAATCCGCCGTCGGAGCTCGATTCCCCCACCACCTCTGCTGGTTTCGAAACCGACCAGCTTCCTCATCTCAGCCACATTTCCGACAACTACCCCGACGAGGACGAAGCTGCCGTTGATCCTCACATCGTTCACGACGAGCCTGATCCCGATGAAGAAGAGGAAGGAGAGGATCTCTACCACGACAATTTCCTGGACGATTATCGGAGGATGGACGACCATGACCAGTACGAGTCCTTGGGCCTCGACGATTCTTTAGAGGACGAGAGGGATTTGGATCAGATTATGGAGGGACAGGCGAGCTGCCGAGATAGAACTGGACACTCGCGATGCTCAGTTCACCCGTCGGGAAGATTCCTGAGCTATTACATGATCAAGACTCGGAGGATGACAATTACAGGGCCTTCAAAAAGAGCAAGAGCTGATTTTCGGCCTCCAGCTGCTGCAAGAAGCTATGATGACATTGATGATCATTCTGTTGATGACCAATATGAGGATGAAGACGATGATGAAAATGAGAATGAGATGTATCGTGTTCAAGGGCCACTTAGAGAACATGTTACCATGGATGCGGTTCGGCGCTGTTATGTGAAAAAGTTCAAAAAATTCTTGGAAACATATGTGAATCCAAAGAGTGGAAATGGTGAGCTTGAGTACATGCGACTGATAAATGAGATGGTGTTAGCCAATAGGTGCAGTTTGGAGATTGATTACAAACAGTTTATCTTTGTCCATCCGAATATTGCAATCTGGTTGGCTGATGCACCCCAGCCCGTCTTAGAAGTTATGGAAGATGTTGCAAAGAAGGTTGTCTTTGACATACATCCAAATTACAAAAATATCCACCAAAAGATCTATGTTCGCATCAATAATTTACCAGTCTATGATCAGATTCGAAATATTAGGCAAATTCATTTAAATACCATGATTCGGAATTGGGGCGTAGTTAAACCCGACGCTCTGGGTCTTCCCCCAGTTGCAGCAGGTGAAGTATGA

Coding sequence (CDS)

ATGGCTGGGCCAACTCCCGATGGAAACTCCGGCAATCCGCCGTCGGAGCTCGATTCCCCCACCACCTCTGCTGGTTTCGAAACCGACCAGCTTCCTCATCTCAGCCACATTTCCGACAACTACCCCGACGAGGACGAAGCTGCCGTTGATCCTCACATCGTTCACGACGAGCCTGATCCCGATGAAGAAGAGGAAGGAGAGGATCTCTACCACGACAATTTCCTGGACGATTATCGGAGGATGGACGACCATGACCAGTACGAGTCCTTGGGCCTCGACGATTCTTTAGAGGACGAGAGGGATTTGGATCAGATTATGGAGGGACAGGCGAGCTGCCGAGATAGAACTGGACACTCGCGATGCTCAGTTCACCCGTCGGGAAGATTCCTGAGCTATTACATGATCAAGACTCGGAGGATGACAATTACAGGGCCTTCAAAAAGAGCAAGAGCTGATTTTCGGCCTCCAGCTGCTGCAAGAAGCTATGATGACATTGATGATCATTCTGTTGATGACCAATATGAGGATGAAGACGATGATGAAAATGAGAATGAGATGTATCGTGTTCAAGGGCCACTTAGAGAACATGTTACCATGGATGCGGTTCGGCGCTGTTATGTGAAAAAGTTCAAAAAATTCTTGGAAACATATGTGAATCCAAAGAGTGGAAATGGTGAGCTTGAGTACATGCGACTGATAAATGAGATGGTGTTAGCCAATAGGTGCAGTTTGGAGATTGATTACAAACAGTTTATCTTTGTCCATCCGAATATTGCAATCTGGTTGGCTGATGCACCCCAGCCCGTCTTAGAAGTTATGGAAGATGTTGCAAAGAAGGTTGTCTTTGACATACATCCAAATTACAAAAATATCCACCAAAAGATCTATGTTCGCATCAATAATTTACCAGTCTATGATCAGATTCGAAATATTAGGCAAATTCATTTAAATACCATGATTCGGAATTGGGGCGTAGTTAAACCCGACGCTCTGGGTCTTCCCCCAGTTGCAGCAGGTGAAGTATGA

Protein sequence

MAGPTPDGNSGNPPSELDSPTTSAGFETDQLPHLSHISDNYPDEDEAAVDPHIVHDEPDPDEEEEGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLEDERDLDQIMEGQASCRDRTGHSRCSVHPSGRFLSYYMIKTRRMTITGPSKRARADFRPPAAARSYDDIDDHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDAVRRCYVKKFKKFLETYVNPKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVAKKVVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIRNWGVVKPDALGLPPVAAGEV
Homology
BLAST of Csor.00g267470 vs. ExPASy Swiss-Prot
Match: Q9LPD9 (DNA replication licensing factor MCM2 OS=Arabidopsis thaliana OX=3702 GN=MCM2 PE=1 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 6.2e-87
Identity = 193/335 (57.61%), Postives = 231/335 (68.96%), Query Frame = 0

Query: 9   NSGNPPSELDSPTTSAGFETDQLP-HLSHISDNYPDEDEAAVDPHIVHDEPD---PDEEE 68
           NS N PS   SP +SAGF TDQLP   S  S+N+ DE+EAAVD  ++ DEPD    +EEE
Sbjct: 5   NSDNEPSSPASP-SSAGFNTDQLPISTSQNSENFSDEEEAAVDTQVIRDEPDEAEDEEEE 64

Query: 69  EGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLEDERDLDQIM------EGQASCRDRTGH 128
           EGEDL++D F++DYR+MD++DQYES G+DDS++DERDL Q M      +     R+    
Sbjct: 65  EGEDLFNDTFMNDYRKMDENDQYESNGIDDSVDDERDLGQAMLDRRAADADLDARENRLA 124

Query: 129 SRCSVH----PSGRFLSYYMIKTRRMTITGPSKRARADFRPPAA-ARSYDDIDDHSVDDQ 188
           +R   H          +Y   K  R T+         D  PP++   S  DI      D 
Sbjct: 125 NRKLPHLLHDNDSDDWNYRPSKRSRTTVPPRGNGGDPDGNPPSSPGVSQPDISMTDQTDD 184

Query: 189 YEDED--DDENENEMYRVQGPLREHVTMDAVRRCYVKKFKKFLETYVNPKSGNGELEYMR 248
           Y+DED  DDE E EMYR+QG LRE V  D VRR   KKFK FL TYV PK+ NG++EY+R
Sbjct: 185 YQDEDDNDDEAEFEMYRIQGTLREWVMRDEVRRFIAKKFKDFLLTYVKPKNENGDIEYVR 244

Query: 249 LINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVAKKVVFDIHPNYKNI 308
           LINEMV AN+CSLEIDYK+FI VHPNIAIWLADAPQPVLEVME+V++KV+FD+HPNYKNI
Sbjct: 245 LINEMVSANKCSLEIDYKEFIHVHPNIAIWLADAPQPVLEVMEEVSEKVIFDLHPNYKNI 304

Query: 309 HQKIYVRINNLPVYDQIRNIRQIHLNTMIRNWGVV 327
           H KIYVR+ NLPV DQIRNIRQIHLNTMIR  GVV
Sbjct: 305 HTKIYVRVTNLPVNDQIRNIRQIHLNTMIRIGGVV 338

BLAST of Csor.00g267470 vs. ExPASy Swiss-Prot
Match: Q2R482 (DNA replication licensing factor MCM2 OS=Oryza sativa subsp. japonica OX=39947 GN=MCM2 PE=1 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 2.0e-85
Identity = 203/370 (54.86%), Postives = 241/370 (65.14%), Query Frame = 0

Query: 7   DGNSGNPPSELDSPTTSAGFETDQLPHLSHIS------DNYPDED--------EAAVDPH 66
           D +  N PS   SP    GF TD+LP  +  S       +Y D+D        EA VDP+
Sbjct: 2   DDSENNAPSTPGSP----GFSTDRLPPNTTTSRGATDPSSYSDDDDDDVVGAEEAEVDPN 61

Query: 67  IVHDEPD---PDEEEEGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLEDERDLDQIM--- 126
           ++ ++      +EEE+GEDL++DN+LDDYRRMD+ DQYES+GLDDS+EDER+LD+IM   
Sbjct: 62  VLPEDDGVVAAEEEEDGEDLFNDNYLDDYRRMDEQDQYESVGLDDSIEDERNLDEIMADR 121

Query: 127 ---EGQASCRD-RTGHSRCSVHPSGRFLSYYMIKTRRMTITGPSKRARADFRP---PAAA 186
              E +   RD RTG +     P    + +       M+   P KR RA+FRP   P   
Sbjct: 122 RAAEAELDARDVRTGAAPDRKLPR---MLHDQDTDEDMSFRRP-KRHRANFRPPREPRTP 181

Query: 187 RSYDDID-----------------------DHSVDDQYEDEDDDENENEMYRVQGPLREH 246
           RS DD D                       D + DD YEDE D+E+E  MYRVQG LRE 
Sbjct: 182 RSDDDGDGATPSSPGRSQRGMYSGGDVPMTDQTDDDPYEDEFDEEDEMNMYRVQGTLREW 241

Query: 247 VTMDAVRRCYVKKFKKFLETYVNPKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHP 306
           VT D VRR   KKFK+FL TYVNPK+  GE EY+RLINEMVLAN+CSLEIDYKQFI++HP
Sbjct: 242 VTRDEVRRFIAKKFKEFLLTYVNPKNEQGEFEYVRLINEMVLANKCSLEIDYKQFIYIHP 301

Query: 307 NIAIWLADAPQPVLEVMEDVAKKVVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHL 327
           NIAIWLADAPQ VLEVME+VAK VVFD+H NY+NIHQKIYVRI NLPVYDQIRNIRQIHL
Sbjct: 302 NIAIWLADAPQSVLEVMEEVAKNVVFDLHKNYRNIHQKIYVRITNLPVYDQIRNIRQIHL 361

BLAST of Csor.00g267470 vs. ExPASy Swiss-Prot
Match: B8BKI8 (DNA replication licensing factor MCM2 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_36121 PE=3 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 3.4e-85
Identity = 202/370 (54.59%), Postives = 241/370 (65.14%), Query Frame = 0

Query: 7   DGNSGNPPSELDSPTTSAGFETDQLPHLSHIS------DNYPDED--------EAAVDPH 66
           D +  N PS   SP    GF TD+LP  +  S       +Y D+D        EA VDP+
Sbjct: 2   DDSENNAPSTPGSP----GFSTDRLPPNTTTSRGATDPSSYSDDDDDDVVGAEEAEVDPN 61

Query: 67  IVHDEPD---PDEEEEGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLEDERDLDQIM--- 126
           ++ ++      +EEE+GEDL++DN+LDDYRRMD+ DQYES+GLDDS+EDER+LD+IM   
Sbjct: 62  VLPEDDGVVAAEEEEDGEDLFNDNYLDDYRRMDEQDQYESVGLDDSIEDERNLDEIMADR 121

Query: 127 ---EGQASCRD-RTGHSRCSVHPSGRFLSYYMIKTRRMTITGPSKRARADFRP---PAAA 186
              E +   RD RTG +     P    + +       M+   P KR RA+FRP   P   
Sbjct: 122 RAAEAELDARDVRTGAAPDRKLPR---MLHDQDTDEDMSFRRP-KRHRANFRPPREPRTP 181

Query: 187 RSYDDID-----------------------DHSVDDQYEDEDDDENENEMYRVQGPLREH 246
           RS DD D                       D + DD YEDE D+E+E  MYRVQG LRE 
Sbjct: 182 RSDDDGDGATPSSPGRSQRGMYSGGDVPMTDQTDDDPYEDEFDEEDEMNMYRVQGTLREW 241

Query: 247 VTMDAVRRCYVKKFKKFLETYVNPKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHP 306
           VT D VRR   KKFK+FL TYVNPK+  GE EY+RLINEMVLAN+CSLEIDYKQFI++HP
Sbjct: 242 VTRDEVRRFIAKKFKEFLLTYVNPKNEQGEFEYVRLINEMVLANKCSLEIDYKQFIYIHP 301

Query: 307 NIAIWLADAPQPVLEVMEDVAKKVVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHL 327
           NIAIWLADAPQ V+EVME+VAK VVFD+H NY+NIHQKIYVRI NLPVYDQIRNIRQIHL
Sbjct: 302 NIAIWLADAPQSVIEVMEEVAKNVVFDLHKNYRNIHQKIYVRITNLPVYDQIRNIRQIHL 361

BLAST of Csor.00g267470 vs. ExPASy Swiss-Prot
Match: Q6DIH3 (DNA replication licensing factor mcm2 OS=Xenopus tropicalis OX=8364 GN=mcm2 PE=2 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 3.8e-20
Identity = 87/288 (30.21%), Postives = 148/288 (51.39%), Query Frame = 0

Query: 42  PDEDEAAVDPHIVHDEPDPDEEEEGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLEDERD 101
           P EDE+     +  DE   +EEE+GE+L  D    DYR + + D+YE+ GLD    DE D
Sbjct: 33  PFEDES---EGMFGDEVPREEEEDGEELIGDAMERDYRAISELDRYEAEGLD----DEDD 92

Query: 102 LDQIMEGQASCRDRTGHSRCSV--HPSGRFLSYYMIKTRRMTITGPSKRARADFRPPAAA 161
           ++ +   Q    ++    R     H  GR     +  +       P+++ R   R    A
Sbjct: 93  VEDLTASQRDAAEQAMRMRDREMGHELGRMRRGLLYDSDEEDEDRPARKRRMAERAAEGA 152

Query: 162 RSYDDIDDHSVDDQYEDEDDDENENEMYRVQG-PLREHVTMDAVRRCYVKKFKKFLETYV 221
                          EDE+  E+   +  ++G  +RE V+M A R     +FK FL T+V
Sbjct: 153 PE-------------EDEEMIESIENLEDMKGHTVREWVSMAATRLEIYHRFKNFLRTHV 212

Query: 222 NPKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVAK 281
           +    +G   +   I++M   N+ SL ++Y+        +A +L +AP  +L++ ++ AK
Sbjct: 213 DE---HGHNVFKEKISDMCKENKESLVVNYEDLAAREHVLAYFLPEAPAEMLKIFDEAAK 272

Query: 282 KVVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIRNWGVV 327
           +VV  ++P Y  I ++I+VRI++LP+ +++R++RQ+HLN +IR  GVV
Sbjct: 273 EVVLVMYPKYDRIAREIHVRISHLPLVEELRSLRQLHLNQLIRTSGVV 297

BLAST of Csor.00g267470 vs. ExPASy Swiss-Prot
Match: P55861 (DNA replication licensing factor mcm2 OS=Xenopus laevis OX=8355 GN=mcm2 PE=1 SV=2)

HSP 1 Score: 99.4 bits (246), Expect = 8.5e-20
Identity = 85/287 (29.62%), Postives = 151/287 (52.61%), Query Frame = 0

Query: 41  YPDEDEAAVDPHIVHDEPDPDEEEEGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLEDER 100
           + DE E      +V     P+EEE+GE+L  D    DYR + + D+YE  GLD    DE 
Sbjct: 34  FEDESEGMFGDGVV-----PEEEEDGEELIGDAMERDYRPISELDRYEVEGLD----DEE 93

Query: 101 DLDQIMEGQASCRDRTGHSRCSVHPSGRFLSYYMIKTRRMTITGPSKRARADFRPPAAAR 160
           D++ +   Q    ++      S+    R +   + + RR  +    +    + RP    R
Sbjct: 94  DVEDLTASQREAAEQ------SMRMRDREMGRELGRMRRGLLYDSDE--EEEDRPARKRR 153

Query: 161 SYDDIDDHSVDDQYEDEDDDENENEMYRVQG-PLREHVTMDAVRRCYVKKFKKFLETYVN 220
             +   + + +   EDE+  E+   +  ++G  +RE V+M A R     +FK FL T+V+
Sbjct: 154 MAERAAEGAPE---EDEEMIESIENLEDMKGHTVREWVSMAATRLEIYHRFKNFLRTHVD 213

Query: 221 PKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVAKK 280
               +G   +   I++M   N+ SL ++Y+        +A +L +AP  +L++ ++ AK+
Sbjct: 214 E---HGHNVFKEKISDMCKENKESLPVNYEDLAAREHVLAYFLPEAPAEMLKIFDEAAKE 273

Query: 281 VVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIRNWGVV 327
           VV  ++P Y  I ++I+VRI++LP+ +++R++RQ+HLN +IR  GVV
Sbjct: 274 VVLVMYPKYDRIAREIHVRISHLPLVEELRSLRQLHLNQLIRTSGVV 297

BLAST of Csor.00g267470 vs. NCBI nr
Match: KAG6571761.1 (DNA replication licensing factor MCM2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 687 bits (1774), Expect = 1.31e-249
Identity = 341/341 (100.00%), Postives = 341/341 (100.00%), Query Frame = 0

Query: 1   MAGPTPDGNSGNPPSELDSPTTSAGFETDQLPHLSHISDNYPDEDEAAVDPHIVHDEPDP 60
           MAGPTPDGNSGNPPSELDSPTTSAGFETDQLPHLSHISDNYPDEDEAAVDPHIVHDEPDP
Sbjct: 1   MAGPTPDGNSGNPPSELDSPTTSAGFETDQLPHLSHISDNYPDEDEAAVDPHIVHDEPDP 60

Query: 61  DEEEEGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLEDERDLDQIMEGQASCRDRTGHSR 120
           DEEEEGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLEDERDLDQIMEGQASCRDRTGHSR
Sbjct: 61  DEEEEGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLEDERDLDQIMEGQASCRDRTGHSR 120

Query: 121 CSVHPSGRFLSYYMIKTRRMTITGPSKRARADFRPPAAARSYDDIDDHSVDDQYEDEDDD 180
           CSVHPSGRFLSYYMIKTRRMTITGPSKRARADFRPPAAARSYDDIDDHSVDDQYEDEDDD
Sbjct: 121 CSVHPSGRFLSYYMIKTRRMTITGPSKRARADFRPPAAARSYDDIDDHSVDDQYEDEDDD 180

Query: 181 ENENEMYRVQGPLREHVTMDAVRRCYVKKFKKFLETYVNPKSGNGELEYMRLINEMVLAN 240
           ENENEMYRVQGPLREHVTMDAVRRCYVKKFKKFLETYVNPKSGNGELEYMRLINEMVLAN
Sbjct: 181 ENENEMYRVQGPLREHVTMDAVRRCYVKKFKKFLETYVNPKSGNGELEYMRLINEMVLAN 240

Query: 241 RCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVAKKVVFDIHPNYKNIHQKIYVRIN 300
           RCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVAKKVVFDIHPNYKNIHQKIYVRIN
Sbjct: 241 RCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVAKKVVFDIHPNYKNIHQKIYVRIN 300

Query: 301 NLPVYDQIRNIRQIHLNTMIRNWGVVKPDALGLPPVAAGEV 341
           NLPVYDQIRNIRQIHLNTMIRNWGVVKPDALGLPPVAAGEV
Sbjct: 301 NLPVYDQIRNIRQIHLNTMIRNWGVVKPDALGLPPVAAGEV 341

BLAST of Csor.00g267470 vs. NCBI nr
Match: KAG7029177.1 (DNA replication licensing factor MCM2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 526 bits (1355), Expect = 2.62e-178
Identity = 284/349 (81.38%), Postives = 291/349 (83.38%), Query Frame = 0

Query: 1   MAGPTPDGNSGNPPSELDSPTTSAGFETDQLPHLSHISDNYPDEDEAAVDPHIVHDEPDP 60
           MAG TPDGNSGNPPSELDSPTTSAGFETDQLPHLSHISDNYPDEDEAAVDPHIVHDEPDP
Sbjct: 1   MAGETPDGNSGNPPSELDSPTTSAGFETDQLPHLSHISDNYPDEDEAAVDPHIVHDEPDP 60

Query: 61  DEEEEGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLEDERDLDQIMEGQ--ASCRDRTGH 120
           DEEEEGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLEDERDLDQIME +  A     T  
Sbjct: 61  DEEEEGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLEDERDLDQIMEDRRAAEMELETRD 120

Query: 121 SRCSVHPSGRFLSYYMIKTRRMTITGPSKRARADFRPPAAARSYDDID------------ 180
           ++ +       L  +  +        PSKR+RADFRPPAAARSYDDID            
Sbjct: 121 AQFTRRKLPELLHDHDSEDDNYR---PSKRSRADFRPPAAARSYDDIDGMQSSPGRSQRE 180

Query: 181 ---------DHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDAVRRCYVKKFKKFLETY 240
                    DHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDAVRR   KKFKKFLETY
Sbjct: 181 NSRDDVPMTDHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDAVRRFIGKKFKKFLETY 240

Query: 241 VNPKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVA 300
           VNPKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQ VLEVMEDVA
Sbjct: 241 VNPKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQSVLEVMEDVA 300

Query: 301 KKVVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIRNWGVV 326
           KKVVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIR  GVV
Sbjct: 301 KKVVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIRIGGVV 346

BLAST of Csor.00g267470 vs. NCBI nr
Match: XP_022972265.1 (DNA replication licensing factor MCM2-like [Cucurbita maxima])

HSP 1 Score: 522 bits (1344), Expect = 1.21e-175
Identity = 281/349 (80.52%), Postives = 291/349 (83.38%), Query Frame = 0

Query: 1   MAGPTPDGNSGNPPSELDSPTTSAGFETDQLPHLSHISDNYPDEDEAAVDPHIVHDEPDP 60
           MAG TPDGNSGNPPSELDSPTTSAGFETDQLPHLSHISDNYPDEDEAAVDPHIVHDEPDP
Sbjct: 1   MAGETPDGNSGNPPSELDSPTTSAGFETDQLPHLSHISDNYPDEDEAAVDPHIVHDEPDP 60

Query: 61  DEEEEGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLEDERDLDQIMEGQ--ASCRDRTGH 120
           DEEEEGEDLYHDNFL+DYRRMDDHDQYESLGLDDSLEDERDLDQIME +  A     T  
Sbjct: 61  DEEEEGEDLYHDNFLEDYRRMDDHDQYESLGLDDSLEDERDLDQIMEDRRAAEMELETRD 120

Query: 121 SRCSVHPSGRFLSYYMIKTRRMTITGPSKRARADFRPPAAARSYDDID------------ 180
           ++ +       L  +  +        PSKR+RADFRPPAAARSYDDID            
Sbjct: 121 AQFTRRKLPELLHDHDSEDDNYR---PSKRSRADFRPPAAARSYDDIDGMQSSPGRSQRE 180

Query: 181 ---------DHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDAVRRCYVKKFKKFLETY 240
                    DHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDAVRR   KKFKKFLETY
Sbjct: 181 NSRDDVPMTDHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDAVRRFIGKKFKKFLETY 240

Query: 241 VNPKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVA 300
           VNPKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQ VLEVMEDVA
Sbjct: 241 VNPKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQSVLEVMEDVA 300

Query: 301 KKVVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIRNWGVV 326
           KKVVFD+HPNYKNIHQKIYVRIN+LPVYDQIRNIRQIHLNTMIR  GVV
Sbjct: 301 KKVVFDLHPNYKNIHQKIYVRINSLPVYDQIRNIRQIHLNTMIRIGGVV 346

BLAST of Csor.00g267470 vs. NCBI nr
Match: XP_022932564.1 (DNA replication licensing factor MCM2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 521 bits (1342), Expect = 2.41e-175
Identity = 281/349 (80.52%), Postives = 289/349 (82.81%), Query Frame = 0

Query: 1   MAGPTPDGNSGNPPSELDSPTTSAGFETDQLPHLSHISDNYPDEDEAAVDPHIVHDEPDP 60
           MAG TPDGNSGNPP ELDSPTTSAGFETDQLPHLSHISDNYPDEDEAAVDPHIVHDEPDP
Sbjct: 1   MAGETPDGNSGNPPLELDSPTTSAGFETDQLPHLSHISDNYPDEDEAAVDPHIVHDEPDP 60

Query: 61  DEEEEGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLEDERDLDQIMEGQ--ASCRDRTGH 120
           DEEEEGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLED+RDLDQIME +  A     T  
Sbjct: 61  DEEEEGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLEDDRDLDQIMEDRRAAEMELETRD 120

Query: 121 SRCSVHPSGRFLSYYMIKTRRMTITGPSKRARADFRPPAAARSYDDID------------ 180
           ++ +       L  +  +        PSKR+RADFRPPAAARSYDDID            
Sbjct: 121 AQFTRRKLPELLHDHDSEDDNYR---PSKRSRADFRPPAAARSYDDIDGMQSSTGRSQRE 180

Query: 181 ---------DHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDAVRRCYVKKFKKFLETY 240
                    DHSVDDQYEDEDDDENENEMYRVQGPLREHVTMD VRR   KKFKKFLETY
Sbjct: 181 NSRDDVPMTDHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDTVRRFIGKKFKKFLETY 240

Query: 241 VNPKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVA 300
           VNPKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQ VLEVMEDVA
Sbjct: 241 VNPKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQSVLEVMEDVA 300

Query: 301 KKVVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIRNWGVV 326
           KKVVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIR  GVV
Sbjct: 301 KKVVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIRIGGVV 346

BLAST of Csor.00g267470 vs. NCBI nr
Match: KAG7018129.1 (DNA replication licensing factor MCM2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 510 bits (1314), Expect = 3.00e-172
Identity = 275/347 (79.25%), Postives = 284/347 (81.84%), Query Frame = 0

Query: 1   MAGPTPDGNSGNPPSELDSPTTSAGFETDQLPHLSHISDNYPDEDEAAVDPHIVHDEPDP 60
           MAGPTPDGNSGNPPS  DSPTTSAGFETDQLPHLS  SD YPDEDEAAVDPH++ DEPDP
Sbjct: 1   MAGPTPDGNSGNPPSTPDSPTTSAGFETDQLPHLSQTSDIYPDEDEAAVDPHVLPDEPDP 60

Query: 61  DEEEEGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLEDERDLDQIMEGQASCRDRTGHSR 120
           DEEEEGEDLYHDNFLDDYRRMD+HDQYESL LDDSLEDERDLDQIME + +       +R
Sbjct: 61  DEEEEGEDLYHDNFLDDYRRMDEHDQYESLDLDDSLEDERDLDQIMEDRRAAEIELD-TR 120

Query: 121 CSVHPSGRFLSYYMIKTRRMTITGPSKRARADFRPPAAARSYDDID-------------- 180
            +     +       +        PSKRARADFRPPAAARSYDDID              
Sbjct: 121 DAQFTRRKLPELLHDQDSEDDNYRPSKRARADFRPPAAARSYDDIDGMQSSPGRSQRETS 180

Query: 181 -------DHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDAVRRCYVKKFKKFLETYVN 240
                  DHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDAVRR   KKFKKFLETYVN
Sbjct: 181 RDDVPITDHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDAVRRFIGKKFKKFLETYVN 240

Query: 241 PKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVAKK 300
           PKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVAKK
Sbjct: 241 PKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVAKK 300

Query: 301 VVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIRNWGVV 326
           VVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIR  GVV
Sbjct: 301 VVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIRIGGVV 346

BLAST of Csor.00g267470 vs. ExPASy TrEMBL
Match: A0A6J1I832 (DNA replication licensing factor MCM2 OS=Cucurbita maxima OX=3661 GN=LOC111470849 PE=3 SV=1)

HSP 1 Score: 522 bits (1344), Expect = 5.85e-176
Identity = 281/349 (80.52%), Postives = 291/349 (83.38%), Query Frame = 0

Query: 1   MAGPTPDGNSGNPPSELDSPTTSAGFETDQLPHLSHISDNYPDEDEAAVDPHIVHDEPDP 60
           MAG TPDGNSGNPPSELDSPTTSAGFETDQLPHLSHISDNYPDEDEAAVDPHIVHDEPDP
Sbjct: 1   MAGETPDGNSGNPPSELDSPTTSAGFETDQLPHLSHISDNYPDEDEAAVDPHIVHDEPDP 60

Query: 61  DEEEEGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLEDERDLDQIMEGQ--ASCRDRTGH 120
           DEEEEGEDLYHDNFL+DYRRMDDHDQYESLGLDDSLEDERDLDQIME +  A     T  
Sbjct: 61  DEEEEGEDLYHDNFLEDYRRMDDHDQYESLGLDDSLEDERDLDQIMEDRRAAEMELETRD 120

Query: 121 SRCSVHPSGRFLSYYMIKTRRMTITGPSKRARADFRPPAAARSYDDID------------ 180
           ++ +       L  +  +        PSKR+RADFRPPAAARSYDDID            
Sbjct: 121 AQFTRRKLPELLHDHDSEDDNYR---PSKRSRADFRPPAAARSYDDIDGMQSSPGRSQRE 180

Query: 181 ---------DHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDAVRRCYVKKFKKFLETY 240
                    DHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDAVRR   KKFKKFLETY
Sbjct: 181 NSRDDVPMTDHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDAVRRFIGKKFKKFLETY 240

Query: 241 VNPKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVA 300
           VNPKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQ VLEVMEDVA
Sbjct: 241 VNPKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQSVLEVMEDVA 300

Query: 301 KKVVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIRNWGVV 326
           KKVVFD+HPNYKNIHQKIYVRIN+LPVYDQIRNIRQIHLNTMIR  GVV
Sbjct: 301 KKVVFDLHPNYKNIHQKIYVRINSLPVYDQIRNIRQIHLNTMIRIGGVV 346

BLAST of Csor.00g267470 vs. ExPASy TrEMBL
Match: A0A6J1EXC1 (DNA replication licensing factor MCM2 OS=Cucurbita moschata OX=3662 GN=LOC111439083 PE=3 SV=1)

HSP 1 Score: 521 bits (1342), Expect = 1.17e-175
Identity = 281/349 (80.52%), Postives = 289/349 (82.81%), Query Frame = 0

Query: 1   MAGPTPDGNSGNPPSELDSPTTSAGFETDQLPHLSHISDNYPDEDEAAVDPHIVHDEPDP 60
           MAG TPDGNSGNPP ELDSPTTSAGFETDQLPHLSHISDNYPDEDEAAVDPHIVHDEPDP
Sbjct: 1   MAGETPDGNSGNPPLELDSPTTSAGFETDQLPHLSHISDNYPDEDEAAVDPHIVHDEPDP 60

Query: 61  DEEEEGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLEDERDLDQIMEGQ--ASCRDRTGH 120
           DEEEEGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLED+RDLDQIME +  A     T  
Sbjct: 61  DEEEEGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLEDDRDLDQIMEDRRAAEMELETRD 120

Query: 121 SRCSVHPSGRFLSYYMIKTRRMTITGPSKRARADFRPPAAARSYDDID------------ 180
           ++ +       L  +  +        PSKR+RADFRPPAAARSYDDID            
Sbjct: 121 AQFTRRKLPELLHDHDSEDDNYR---PSKRSRADFRPPAAARSYDDIDGMQSSTGRSQRE 180

Query: 181 ---------DHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDAVRRCYVKKFKKFLETY 240
                    DHSVDDQYEDEDDDENENEMYRVQGPLREHVTMD VRR   KKFKKFLETY
Sbjct: 181 NSRDDVPMTDHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDTVRRFIGKKFKKFLETY 240

Query: 241 VNPKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVA 300
           VNPKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQ VLEVMEDVA
Sbjct: 241 VNPKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQSVLEVMEDVA 300

Query: 301 KKVVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIRNWGVV 326
           KKVVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIR  GVV
Sbjct: 301 KKVVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIRIGGVV 346

BLAST of Csor.00g267470 vs. ExPASy TrEMBL
Match: A0A6J1F9C4 (DNA replication licensing factor MCM2 OS=Cucurbita moschata OX=3662 GN=LOC111442009 PE=3 SV=1)

HSP 1 Score: 510 bits (1314), Expect = 1.86e-171
Identity = 275/347 (79.25%), Postives = 284/347 (81.84%), Query Frame = 0

Query: 1   MAGPTPDGNSGNPPSELDSPTTSAGFETDQLPHLSHISDNYPDEDEAAVDPHIVHDEPDP 60
           MAGPTPDGNSGNPPS  DSPTTSAGFETDQLPHLS  SD YPDEDEAAVDPH++ DEPDP
Sbjct: 1   MAGPTPDGNSGNPPSTPDSPTTSAGFETDQLPHLSQTSDIYPDEDEAAVDPHVLPDEPDP 60

Query: 61  DEEEEGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLEDERDLDQIMEGQASCRDRTGHSR 120
           DEEEEGEDLYHDNFLDDYRRMD+HDQYESL LDDSLEDERDLDQIME + +       +R
Sbjct: 61  DEEEEGEDLYHDNFLDDYRRMDEHDQYESLDLDDSLEDERDLDQIMEDRRAAEIELD-TR 120

Query: 121 CSVHPSGRFLSYYMIKTRRMTITGPSKRARADFRPPAAARSYDDID-------------- 180
            +     +       +        PSKRARADFRPPAAARSYDDID              
Sbjct: 121 DAQFTRRKLPELLHDQDSEDDNYRPSKRARADFRPPAAARSYDDIDGMQSSPGRSQRETS 180

Query: 181 -------DHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDAVRRCYVKKFKKFLETYVN 240
                  DHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDAVRR   KKFKKFLETYVN
Sbjct: 181 RDDVPITDHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDAVRRFIGKKFKKFLETYVN 240

Query: 241 PKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVAKK 300
           PKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVAKK
Sbjct: 241 PKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVAKK 300

Query: 301 VVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIRNWGVV 326
           VVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIR  GVV
Sbjct: 301 VVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIRIGGVV 346

BLAST of Csor.00g267470 vs. ExPASy TrEMBL
Match: A0A6J1F4F0 (DNA replication licensing factor MCM2 OS=Cucurbita moschata OX=3662 GN=LOC111442009 PE=3 SV=1)

HSP 1 Score: 510 bits (1314), Expect = 1.97e-171
Identity = 275/347 (79.25%), Postives = 284/347 (81.84%), Query Frame = 0

Query: 1   MAGPTPDGNSGNPPSELDSPTTSAGFETDQLPHLSHISDNYPDEDEAAVDPHIVHDEPDP 60
           MAGPTPDGNSGNPPS  DSPTTSAGFETDQLPHLS  SD YPDEDEAAVDPH++ DEPDP
Sbjct: 1   MAGPTPDGNSGNPPSTPDSPTTSAGFETDQLPHLSQTSDIYPDEDEAAVDPHVLPDEPDP 60

Query: 61  DEEEEGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLEDERDLDQIMEGQASCRDRTGHSR 120
           DEEEEGEDLYHDNFLDDYRRMD+HDQYESL LDDSLEDERDLDQIME + +       +R
Sbjct: 61  DEEEEGEDLYHDNFLDDYRRMDEHDQYESLDLDDSLEDERDLDQIMEDRRAAEIELD-TR 120

Query: 121 CSVHPSGRFLSYYMIKTRRMTITGPSKRARADFRPPAAARSYDDID-------------- 180
            +     +       +        PSKRARADFRPPAAARSYDDID              
Sbjct: 121 DAQFTRRKLPELLHDQDSEDDNYRPSKRARADFRPPAAARSYDDIDGMQSSPGRSQRETS 180

Query: 181 -------DHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDAVRRCYVKKFKKFLETYVN 240
                  DHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDAVRR   KKFKKFLETYVN
Sbjct: 181 RDDVPITDHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDAVRRFIGKKFKKFLETYVN 240

Query: 241 PKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVAKK 300
           PKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVAKK
Sbjct: 241 PKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVAKK 300

Query: 301 VVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIRNWGVV 326
           VVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIR  GVV
Sbjct: 301 VVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIRIGGVV 346

BLAST of Csor.00g267470 vs. ExPASy TrEMBL
Match: A0A6J1J0A6 (DNA replication licensing factor MCM2 OS=Cucurbita maxima OX=3661 GN=LOC111481534 PE=3 SV=1)

HSP 1 Score: 509 bits (1310), Expect = 7.42e-171
Identity = 274/347 (78.96%), Postives = 284/347 (81.84%), Query Frame = 0

Query: 1   MAGPTPDGNSGNPPSELDSPTTSAGFETDQLPHLSHISDNYPDEDEAAVDPHIVHDEPDP 60
           MAGPTPDGNSGNPPS  DSPTTSAGFETDQLPHLS  SD YPDEDEAAVDPH++ DEPDP
Sbjct: 1   MAGPTPDGNSGNPPSTPDSPTTSAGFETDQLPHLSQTSDIYPDEDEAAVDPHVLPDEPDP 60

Query: 61  DEEEEGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLEDERDLDQIMEGQASCRDRTGHSR 120
           DEEEEGEDLYHDNFLDDYRRMD+HDQYESL LDDSLEDERDLDQIME + +       +R
Sbjct: 61  DEEEEGEDLYHDNFLDDYRRMDEHDQYESLDLDDSLEDERDLDQIMEDRRAAEIELD-TR 120

Query: 121 CSVHPSGRFLSYYMIKTRRMTITGPSKRARADFRPPAAARSYDDID-------------- 180
            +     +       +        PSKRARADFRPPAAARSYD+ID              
Sbjct: 121 DAQFTRRKLPELLHDQDSEDDNYRPSKRARADFRPPAAARSYDEIDGMQSSPGRSQRENS 180

Query: 181 -------DHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDAVRRCYVKKFKKFLETYVN 240
                  DHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDAVRR   KKFKKFLETYVN
Sbjct: 181 RDDVPITDHSVDDQYEDEDDDENENEMYRVQGPLREHVTMDAVRRFIGKKFKKFLETYVN 240

Query: 241 PKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVAKK 300
           PKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVAKK
Sbjct: 241 PKSGNGELEYMRLINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVAKK 300

Query: 301 VVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIRNWGVV 326
           VVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIR  GVV
Sbjct: 301 VVFDIHPNYKNIHQKIYVRINNLPVYDQIRNIRQIHLNTMIRIGGVV 346

BLAST of Csor.00g267470 vs. TAIR 10
Match: AT1G44900.2 (minichromosome maintenance (MCM2/3/5) family protein )

HSP 1 Score: 323.2 bits (827), Expect = 2.6e-88
Identity = 193/333 (57.96%), Postives = 231/333 (69.37%), Query Frame = 0

Query: 9   NSGNPPSELDSPTTSAGFETDQLP-HLSHISDNYPDEDEAAVDPHIVHDEPD---PDEEE 68
           NS N PS   SP +SAGF TDQLP   S  S+N+ DE+EAAVD  ++ DEPD    +EEE
Sbjct: 5   NSDNEPSSPASP-SSAGFNTDQLPISTSQNSENFSDEEEAAVDTQVIRDEPDEAEDEEEE 64

Query: 69  EGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLEDERDLDQIM------EGQASCRDRTGH 128
           EGEDL++D F++DYR+MD++DQYES G+DDS++DERDL Q M      +     R+    
Sbjct: 65  EGEDLFNDTFMNDYRKMDENDQYESNGIDDSVDDERDLGQAMLDRRAADADLDARENRLA 124

Query: 129 SRCSVH--PSGRFLSYYMIKTRRMTITGPSKRARADFRPPAA-ARSYDDIDDHSVDDQYE 188
           +R   H        +Y   K  R T+         D  PP++   S  DI      D Y+
Sbjct: 125 NRKLPHLLHDNDDWNYRPSKRSRTTVPPRGNGGDPDGNPPSSPGVSQPDISMTDQTDDYQ 184

Query: 189 DED--DDENENEMYRVQGPLREHVTMDAVRRCYVKKFKKFLETYVNPKSGNGELEYMRLI 248
           DED  DDE E EMYR+QG LRE V  D VRR   KKFK FL TYV PK+ NG++EY+RLI
Sbjct: 185 DEDDNDDEAEFEMYRIQGTLREWVMRDEVRRFIAKKFKDFLLTYVKPKNENGDIEYVRLI 244

Query: 249 NEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVAKKVVFDIHPNYKNIHQ 308
           NEMV AN+CSLEIDYK+FI VHPNIAIWLADAPQPVLEVME+V++KV+FD+HPNYKNIH 
Sbjct: 245 NEMVSANKCSLEIDYKEFIHVHPNIAIWLADAPQPVLEVMEEVSEKVIFDLHPNYKNIHT 304

Query: 309 KIYVRINNLPVYDQIRNIRQIHLNTMIRNWGVV 327
           KIYVR+ NLPV DQIRNIRQIHLNTMIR  GVV
Sbjct: 305 KIYVRVTNLPVNDQIRNIRQIHLNTMIRIGGVV 336

BLAST of Csor.00g267470 vs. TAIR 10
Match: AT1G44900.1 (minichromosome maintenance (MCM2/3/5) family protein )

HSP 1 Score: 322.4 bits (825), Expect = 4.4e-88
Identity = 193/335 (57.61%), Postives = 231/335 (68.96%), Query Frame = 0

Query: 9   NSGNPPSELDSPTTSAGFETDQLP-HLSHISDNYPDEDEAAVDPHIVHDEPD---PDEEE 68
           NS N PS   SP +SAGF TDQLP   S  S+N+ DE+EAAVD  ++ DEPD    +EEE
Sbjct: 5   NSDNEPSSPASP-SSAGFNTDQLPISTSQNSENFSDEEEAAVDTQVIRDEPDEAEDEEEE 64

Query: 69  EGEDLYHDNFLDDYRRMDDHDQYESLGLDDSLEDERDLDQIM------EGQASCRDRTGH 128
           EGEDL++D F++DYR+MD++DQYES G+DDS++DERDL Q M      +     R+    
Sbjct: 65  EGEDLFNDTFMNDYRKMDENDQYESNGIDDSVDDERDLGQAMLDRRAADADLDARENRLA 124

Query: 129 SRCSVH----PSGRFLSYYMIKTRRMTITGPSKRARADFRPPAA-ARSYDDIDDHSVDDQ 188
           +R   H          +Y   K  R T+         D  PP++   S  DI      D 
Sbjct: 125 NRKLPHLLHDNDSDDWNYRPSKRSRTTVPPRGNGGDPDGNPPSSPGVSQPDISMTDQTDD 184

Query: 189 YEDED--DDENENEMYRVQGPLREHVTMDAVRRCYVKKFKKFLETYVNPKSGNGELEYMR 248
           Y+DED  DDE E EMYR+QG LRE V  D VRR   KKFK FL TYV PK+ NG++EY+R
Sbjct: 185 YQDEDDNDDEAEFEMYRIQGTLREWVMRDEVRRFIAKKFKDFLLTYVKPKNENGDIEYVR 244

Query: 249 LINEMVLANRCSLEIDYKQFIFVHPNIAIWLADAPQPVLEVMEDVAKKVVFDIHPNYKNI 308
           LINEMV AN+CSLEIDYK+FI VHPNIAIWLADAPQPVLEVME+V++KV+FD+HPNYKNI
Sbjct: 245 LINEMVSANKCSLEIDYKEFIHVHPNIAIWLADAPQPVLEVMEEVSEKVIFDLHPNYKNI 304

Query: 309 HQKIYVRINNLPVYDQIRNIRQIHLNTMIRNWGVV 327
           H KIYVR+ NLPV DQIRNIRQIHLNTMIR  GVV
Sbjct: 305 HTKIYVRVTNLPVNDQIRNIRQIHLNTMIRIGGVV 338

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LPD96.2e-8757.61DNA replication licensing factor MCM2 OS=Arabidopsis thaliana OX=3702 GN=MCM2 PE... [more]
Q2R4822.0e-8554.86DNA replication licensing factor MCM2 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
B8BKI83.4e-8554.59DNA replication licensing factor MCM2 OS=Oryza sativa subsp. indica OX=39946 GN=... [more]
Q6DIH33.8e-2030.21DNA replication licensing factor mcm2 OS=Xenopus tropicalis OX=8364 GN=mcm2 PE=2... [more]
P558618.5e-2029.62DNA replication licensing factor mcm2 OS=Xenopus laevis OX=8355 GN=mcm2 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
KAG6571761.11.31e-249100.00DNA replication licensing factor MCM2, partial [Cucurbita argyrosperma subsp. so... [more]
KAG7029177.12.62e-17881.38DNA replication licensing factor MCM2 [Cucurbita argyrosperma subsp. argyrosperm... [more]
XP_022972265.11.21e-17580.52DNA replication licensing factor MCM2-like [Cucurbita maxima][more]
XP_022932564.12.41e-17580.52DNA replication licensing factor MCM2-like isoform X1 [Cucurbita moschata][more]
KAG7018129.13.00e-17279.25DNA replication licensing factor MCM2 [Cucurbita argyrosperma subsp. argyrosperm... [more]
Match NameE-valueIdentityDescription
A0A6J1I8325.85e-17680.52DNA replication licensing factor MCM2 OS=Cucurbita maxima OX=3661 GN=LOC11147084... [more]
A0A6J1EXC11.17e-17580.52DNA replication licensing factor MCM2 OS=Cucurbita moschata OX=3662 GN=LOC111439... [more]
A0A6J1F9C41.86e-17179.25DNA replication licensing factor MCM2 OS=Cucurbita moschata OX=3662 GN=LOC111442... [more]
A0A6J1F4F01.97e-17179.25DNA replication licensing factor MCM2 OS=Cucurbita moschata OX=3662 GN=LOC111442... [more]
A0A6J1J0A67.42e-17178.96DNA replication licensing factor MCM2 OS=Cucurbita maxima OX=3661 GN=LOC11148153... [more]
Match NameE-valueIdentityDescription
AT1G44900.22.6e-8857.96minichromosome maintenance (MCM2/3/5) family protein [more]
AT1G44900.14.4e-8857.61minichromosome maintenance (MCM2/3/5) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.1640.10coord: 200..304
e-value: 2.5E-30
score: 106.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 8..35
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..73
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 38..56
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 168..183
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 145..183
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 153..167
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 57..71
NoneNo IPR availablePANTHERPTHR11630:SF101DNA HELICASEcoord: 125..327
IPR027925MCM N-terminal domainPFAMPF14551MCM_Ncoord: 208..301
e-value: 1.1E-14
score: 54.8
IPR008045DNA replication licensing factor Mcm2PFAMPF12619MCM2_Ncoord: 44..180
e-value: 4.0E-9
score: 36.9
IPR031327Mini-chromosome maintenance proteinPANTHERPTHR11630DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBERcoord: 125..327
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 209..326

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g267470.m01Csor.00g267470.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006268 DNA unwinding involved in DNA replication
biological_process GO:0000727 double-strand break repair via break-induced replication
biological_process GO:1902975 mitotic DNA replication initiation
biological_process GO:1905775 negative regulation of DNA helicase activity
biological_process GO:0032508 DNA duplex unwinding
biological_process GO:0006270 DNA replication initiation
cellular_component GO:0042555 MCM complex
cellular_component GO:0000347 THO complex
cellular_component GO:0005634 nucleus
molecular_function GO:0043138 3'-5' DNA helicase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003697 single-stranded DNA binding
molecular_function GO:0017116 single-stranded DNA helicase activity
molecular_function GO:0003677 DNA binding