Csor.00g266060 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g266060
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionE3 ubiquitin-protein ligase MIEL1-like
LocationCsor_Chr17: 9138581 .. 9141469 (+)
RNA-Seq ExpressionCsor.00g266060
SyntenyCsor.00g266060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAACAAGAGGACTCCCCTGTGCCCCAATCTGCCGGGAGAGAGGATTTCGGAAAATTTCAGCACGGGTATCGATTTCTGTATCATTTGTTGTTGTTCTTCTTCTTTGTTCTCGCTTTTGTTATTAGTAACTTGTTTTCGATTTCAATTAGATGCGAGCATTATAGGAGGCGTTGCAGAATCCGAGCCCCATGTTGCGACCGAATCTTTACCTGTCGCCATTGCCACAACGAGGCCATGGTATGTCTCTTTTTTTTCTATGCCTTTGTGTTGCGTGCGTTTGTTTGTGAGAGATGTATTAGAAAGGATTTTGCATATGCTGTTAAACTCGAACTTTCTGTAATTTTCTCTAGTAGTTTTCTGAATTTTCTGAAATTTTCGTTGATTTTAGACTTCGCTGAGCAATCCTAAGGACCGTCACGAACTCGTTCGGCAGGACGTTAGACAAGTCTGTTTGTACGCTGTTCTATTGTTTTTTTTTTTCTTTTTTGTGAATATGACATCTTTTAGAGCATATCGTGATGTGAATTTATTAATTTTGTTGGAATTGTTGCTTTAGGTGGTTTGCTTGATTTGCAACACAGAGCAAGAGGTTTGGGGGAGATTTTTTTTGTTGTTCTTGGGATAATTTCCATTCCTTGCGTTTAGATATTCTTGTTTTTTGCTCTGCTAAGCGTTTATATTTGGTTTAATATAGGTTGCTAAGTCTTGTTGCAAGTGCAATGTTAATATGGGAGAATACTTCTGCGGCATCTGCAAATTCTACGATGATGATGTAGGAGAAGTTTCCATCAATCTGTTTCTTTCTTACTTTGTTCTGTTTCGCTTTCTTTAAGAACTAATTTTCAGTTTTAAACTGTGGAACCGAATGGTTTTCTTATCCTAGATGCTAATAGATTCTGCTATGAATGCAGACCAGGAAAAAGCAGTTTCATTGTGATGCGTGTGGTATCTGTAGGTTCGTGTGCATATTTCCCGTTCTAGTTCTAATGTAATCTCAGCTTTCTGTGCTAAGTTTCTCAACACGTGTTCTTTGTTATATGATTTTCAGGGTTGGTGGTCGTGAAAATTTCTTTCACTGTGAAAAATGTGGTATGTCATTCAATTCTCCTGATGGTTAGTTAGAAATACACATTGTGTTATTCATTTACTGTTTGAGGTAAATATTGCAAAAGGATTTCTCTGGTTTGTCTGTAAAGTCTTTGATACTATGTAATAAAACAGGCACACACATTGAGGCCATATGAGACTGGAACAATTAGTAAAATGTGAGCGATTGAGCGAATAAACCGTTCAGATGAATTAACTTTTTTTTTTTTTTTTTTTTATAAATACAGGATCTTGCTATTCCACTCCTCTGCGTGATAACCACATGTGTGTTGAGAACTCCATGAAAAGTTTTTGCCCCATCTGTTATGAGGTTTGTCCTTTTAGAATGCATCTTTTCTCAGGGAAGTATAATCTAAACTGTTCTCTTCTAACAATTTTATTGATGCTTGCAGTTTCTTTTTGACTCAATAAAAGACACAACTGTTATGCCTTGTGGTCACACAATACATTTGGAGTGCTTCAGAGAAATGGAATCACAGAATCAGTAATCCAAACTCCAGTTTCTGAATCTACTATTGAACATGTTTCTAAAAACTTTGAATATGCTACATACAACTCTATTTTCTATTATATCCATTGCCTTTTATTCTCAAACCAAACTGGTTCTAAGTTTCCTGTTTTGAATTACATTAAGATTTCCAGTTCTCTTGTAATAATGAAGTTGTATCTGTCTCTCAGATACCGTTGTCCTATTTGCTCGAAGACAGTTATGGACATGTCTGCAAGCTGGGCATTGCTGAAAATGGAGGTGTGTTACATCTTAGTTTAGGCGTGATAAAAGTGGCATGTAGAAGGCAATTAGATTACTGTCTTTTTTATCCATAGAGCAGTTTCCCCAGTTAGTGAATATGGTAGAACAGGTGGCTTCACGTAAATTTTAGCAACCTTAGAAATTAGAATATATATCTTATCATTGAACATTGTACCCACTCTTCAACCTCCTGACCGTTCTTTTCGTTTCTTTCTTTGTCTTGATGTATGATTACATGTATTGGGCTGATTCTTTGAAAGTTGATGGTTGCAGACTCAATATCTCAATAGAATTCTAACTGAGTGATTGCATGAAGAAAGAGATTTAAGAAATGAGTGTGGATTCAGGGCTTTAGTTATGCCTTAAAGTTGAGTTTAGAGGGATATAGAGGCATTAACATATATAATTAAAAATGAAAGAGGAAGATGTCCGTGAGAGCTATATATATTCTGTTACAGACAAGTACTTGACTTGATCTTATACGACCTTAAATCATGTTCGTCCACTTGAATAAAATTTTCTCTTATTTTTATTTTTCCCTCATTTCCCATTTACCCATTACCTGTTATCAAGTTGTTATTGGATGGTAAAATAGATCTTATTGGATGCTTACTTTTTGGTATTCCAAGTGGTATTTCTATCTATTTGCAAATTCTGACATGTTTCTTCTGCCATATTCTGGTGTCGAAATTCCGTAGATTTTTGGTTATCTTAATTATGTTTTCTTTACCATAGATCTTTTGCGATATTATCTTAAATTTGAAAATTGTTTCTATCTTATCTAGATTGACTGCACACCGATGCCGGAGGAGTACAGCCACGAGGTAGTTGCTTGTGTACATGTTTAGATTACCGCAAGAATGAAATCTTCTAACTTAACTGTGTACCTGGCTAAACAGGTATCAATTCTTTGCAACGACTGCAGCCATACGAGCAAAGTTCGTTTTCACATTTTGGGACACAAGTGCAGTCAGTGCAATTCATTCAATACTAGGAGGATTTCTACTGGAGACGTCAATGAAAACTGA

mRNA sequence

ATGGAACAAGAGGACTCCCCTGTGCCCCAATCTGCCGGGAGAGAGGATTTCGGAAAATTTCAGCACGGATGCGAGCATTATAGGAGGCGTTGCAGAATCCGAGCCCCATGTTGCGACCGAATCTTTACCTGTCGCCATTGCCACAACGAGGCCATGACTTCGCTGAGCAATCCTAAGGACCGTCACGAACTCGTTCGGCAGGACGTTAGACAAGTGGTTTGCTTGATTTGCAACACAGAGCAAGAGGTTGCTAAGTCTTGTTGCAAGTGCAATGTTAATATGGGAGAATACTTCTGCGGCATCTGCAAATTCTACGATGATGATACCAGGAAAAAGCAGTTTCATTGTGATGCGTGTGGTATCTGTAGGGTTGGTGGTCGTGAAAATTTCTTTCACTGTGAAAAATGTGGATCTTGCTATTCCACTCCTCTGCGTGATAACCACATGTGTGTTGAGAACTCCATGAAAAGTTTTTGCCCCATCTGTTATGAGTTTCTTTTTGACTCAATAAAAGACACAACTGTTATGCCTTGTGGTCACACAATACATTTGGAGTGCTTCAGAGAAATGGAATCACAGAATCAATACCGTTGTCCTATTTGCTCGAAGACAGTTATGGACATGTCTGCAAGCTGGGCATTGCTGAAAATGGAGATTGACTGCACACCGATGCCGGAGGAGTACAGCCACGAGGTATCAATTCTTTGCAACGACTGCAGCCATACGAGCAAAGTTCGTTTTCACATTTTGGGACACAAGTGCAGTCAGTGCAATTCATTCAATACTAGGAGGATTTCTACTGGAGACGTCAATGAAAACTGA

Coding sequence (CDS)

ATGGAACAAGAGGACTCCCCTGTGCCCCAATCTGCCGGGAGAGAGGATTTCGGAAAATTTCAGCACGGATGCGAGCATTATAGGAGGCGTTGCAGAATCCGAGCCCCATGTTGCGACCGAATCTTTACCTGTCGCCATTGCCACAACGAGGCCATGACTTCGCTGAGCAATCCTAAGGACCGTCACGAACTCGTTCGGCAGGACGTTAGACAAGTGGTTTGCTTGATTTGCAACACAGAGCAAGAGGTTGCTAAGTCTTGTTGCAAGTGCAATGTTAATATGGGAGAATACTTCTGCGGCATCTGCAAATTCTACGATGATGATACCAGGAAAAAGCAGTTTCATTGTGATGCGTGTGGTATCTGTAGGGTTGGTGGTCGTGAAAATTTCTTTCACTGTGAAAAATGTGGATCTTGCTATTCCACTCCTCTGCGTGATAACCACATGTGTGTTGAGAACTCCATGAAAAGTTTTTGCCCCATCTGTTATGAGTTTCTTTTTGACTCAATAAAAGACACAACTGTTATGCCTTGTGGTCACACAATACATTTGGAGTGCTTCAGAGAAATGGAATCACAGAATCAATACCGTTGTCCTATTTGCTCGAAGACAGTTATGGACATGTCTGCAAGCTGGGCATTGCTGAAAATGGAGATTGACTGCACACCGATGCCGGAGGAGTACAGCCACGAGGTATCAATTCTTTGCAACGACTGCAGCCATACGAGCAAAGTTCGTTTTCACATTTTGGGACACAAGTGCAGTCAGTGCAATTCATTCAATACTAGGAGGATTTCTACTGGAGACGTCAATGAAAACTGA

Protein sequence

MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKDRHELVRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN
Homology
[Close]
BLAST of Csor.00g266060 vs. ExPASy Swiss-Prot
Match: Q8VZK0 (E3 ubiquitin-protein ligase MIEL1 OS=Arabidopsis thaliana OX=3702 GN=MIEL1 PE=1 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 4.5e-104
Identity = 161/254 (63.39%), Postives = 202/254 (79.53%), Query Frame = 0 

Query: 14  REDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKDRHELVRQDVRQVV 73
           R  FGK   GC+HY+RRC+IRAPCC+ +F CRHCHNE+ ++L N  DRH+LVRQDV+QV+
Sbjct: 8   RLHFGKMGFGCKHYKRRCQIRAPCCNEVFDCRHCHNESTSTLRNIYDRHDLVRQDVKQVI 67

Query: 74  CLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACGICRVGGRENFFHC 133
           C +C+TEQ  A+ C  C VNMGEYFC IC FYDDDT K+QFHCD CGICRVGGRENFFHC
Sbjct: 68  CSVCDTEQPAAQVCSNCGVNMGEYFCSICIFYDDDTEKQQFHCDDCGICRVGGRENFFHC 127

Query: 134 EKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQ 193
           +KCGSCY+  LR+NH CVENSM+  CPICYE+LFDS+KDT VM CGHT+H+EC+ EM  +
Sbjct: 128 KKCGSCYAVGLRNNHRCVENSMRHHCPICYEYLFDSLKDTNVMKCGHTMHVECYNEMIKR 187

Query: 194 NQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEY-SHEVSILCNDCSHTSKVRFHILGH 253
           +++ CPICS++V+DMS +W  L  EI+ T MP +Y   +V ILCNDC+ T++V FHI+G 
Sbjct: 188 DKFCCPICSRSVIDMSKTWQRLDEEIEATAMPSDYRDKKVWILCNDCNDTTEVHFHIIGQ 247

Query: 254 KCSQCNSFNTRRIS 267
           KC  C S+NTR I+
Sbjct: 248 KCGHCRSYNTRAIA 261

[Close]
BLAST of Csor.00g266060 vs. ExPASy Swiss-Prot
Match: Q9FFB6 (E3 ubiquitin-protein ligase RZFP34 OS=Arabidopsis thaliana OX=3702 GN=RZPF34 PE=1 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 9.6e-91
Identity = 144/267 (53.93%), Postives = 184/267 (68.91%), Query Frame = 0 

Query: 4   EDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLS-NPKDRH 63
           E+    + A   + G   +GC HYRRRC+IRAPCCD IF CRHCHNEA  SL      RH
Sbjct: 7   ENEQNQEFANLMEIGSGHYGCSHYRRRCKIRAPCCDEIFDCRHCHNEAKDSLHIEQHHRH 66

Query: 64  ELVRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACGIC 123
           EL R +V +V+C +C TEQ+V ++C  C V MG+YFC  CKF+DDD  KKQ+HCD CGIC
Sbjct: 67  ELPRHEVSKVICSLCETEQDVQQNCSNCGVCMGKYFCSKCKFFDDDLSKKQYHCDECGIC 126

Query: 124 RVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTI 183
           R GG ENFFHC++C  CYS  + D H CVE +M   CP+C+E+LFDS +D TV+ CGHT+
Sbjct: 127 RTGGEENFFHCKRCRCCYSKIMEDKHQCVEGAMHHNCPVCFEYLFDSTRDITVLRCGHTM 186

Query: 184 HLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHE-VSILCNDCSH 243
           HLEC ++M   N+Y CP+CSK++ DMS  W  L  E+   PMP+ Y ++ V ILCNDC  
Sbjct: 187 HLECTKDMGLHNRYTCPVCSKSICDMSNLWKKLDEEVAAYPMPKMYENKMVWILCNDCGS 246

Query: 244 TSKVRFHILGHKCSQCNSFNTRRISTG 269
            + VRFH++ HKCS C S+NTR+   G
Sbjct: 247 NTNVRFHLIAHKCSSCGSYNTRQTQRG 273

[Close]
BLAST of Csor.00g266060 vs. ExPASy Swiss-Prot
Match: Q5JL96 (Probable E3 ubiquitin-protein ligase RZFP34 OS=Oryza sativa subsp. japonica OX=39947 GN=RZFP34 PE=2 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 3.1e-89
Identity = 143/265 (53.96%), Postives = 188/265 (70.94%), Query Frame = 0 

Query: 2   EQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLS-NPKD 61
           E+ +   P    + + G  Q+GC HYRRRCRIRAPCC+ IF CRHCHNE   S+  +   
Sbjct: 39  EKTEDVDPDDYEKLEEGIMQYGCAHYRRRCRIRAPCCNEIFDCRHCHNETKNSIKIDAVK 98

Query: 62  RHELVRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 121
           RHEL R +V+QV+C +C TEQEV + C  C V MG+YFC +CK +DDD  K+Q+HC+ CG
Sbjct: 99  RHELPRHEVQQVICSLCGTEQEVRQVCISCGVCMGKYFCEVCKLFDDDVSKQQYHCNGCG 158

Query: 122 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 181
           ICR+GG+ENFFHC KCG CYS  L+++H CVE +M   CPIC+E+LF+S  D +V+PCGH
Sbjct: 159 ICRIGGKENFFHCSKCGCCYSIVLKNSHACVEGAMHHDCPICFEYLFESTNDVSVLPCGH 218

Query: 182 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 241
           TIH++C REME   Q+ CP+CSK+V DMS +W  L  E+  T      +  V ILCNDC 
Sbjct: 219 TIHVKCLREMEEHCQFACPLCSKSVCDMSKAWERLDEEL-ATISDTCDNKMVRILCNDCG 278

Query: 242 HTSKVRFHILGHKCSQCNSFNTRRI 266
            TS+V+FH++ HKC +C S+NTR+I
Sbjct: 279 ATSEVQFHLIAHKCQKCKSYNTRQI 302

[Close]
BLAST of Csor.00g266060 vs. ExPASy Swiss-Prot
Match: Q10LI1 (E3 ubiquitin-protein ligase SRFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SRFP1 PE=1 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 3.4e-88
Identity = 141/255 (55.29%), Postives = 179/255 (70.20%), Query Frame = 0 

Query: 12  AGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKDRHELVRQDVRQ 71
           A R  FG+   GC+HYRRRCRIRAPCC+ +F CRHCHNE      + KD HEL R  V  
Sbjct: 6   ASRHGFGRMGFGCKHYRRRCRIRAPCCNDVFHCRHCHNE------STKDGHELDRHAVES 65

Query: 72  VVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACGICRVGGRENFF 131
           V+CL+C+TEQ VA+ C  C V MGEYFC  CKF+DDD  ++ FHC  CGICRVGG++NFF
Sbjct: 66  VICLVCDTEQPVAQVCYNCGVCMGEYFCSACKFFDDDVDREHFHCQDCGICRVGGKDNFF 125

Query: 132 HCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREME 191
           HCEKCGSCYS  LRD H C+ENSMK+ CPICYE+LFDS+++T+V+ CGHT+HL+CF EM 
Sbjct: 126 HCEKCGSCYSVSLRDKHCCIENSMKNNCPICYEYLFDSLRETSVLRCGHTMHLQCFHEML 185

Query: 192 SQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSKVRFHILG 251
             +++ CPICS  + DM      L  EI+   +  +Y  +  I+CNDC  T++V   + G
Sbjct: 186 KHDKFSCPICSMPIFDMDKFLRALDAEIEANMLHIDYMGKGWIVCNDCRDTTQVYARVAG 245

Query: 252 HKCSQCNSFNTRRIS 267
           HKC  C S NT R++
Sbjct: 246 HKCCHCQSHNTCRVA 254

[Close]
BLAST of Csor.00g266060 vs. ExPASy Swiss-Prot
Match: Q9CR50 (RING finger and CHY zinc finger domain-containing protein 1 OS=Mus musculus OX=10090 GN=Rchy1 PE=1 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 1.5e-64
Identity = 112/251 (44.62%), Postives = 155/251 (61.75%), Query Frame = 0 

Query: 13  GREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKDRHELVRQDVRQV 72
           G  +  +   GCEHY R C ++APCCD+++TCR CH        +  + H+L R  V++V
Sbjct: 9   GVRNLAQGPRGCEHYDRACLLKAPCCDKLYTCRLCH--------DTNEDHQLDRFKVKEV 68

Query: 73  VCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACGICRVGGRENFFH 132
            C+ C   Q   ++C  C+   GEY+C IC  +D D  K+Q+HC++CGICR+G +E+FFH
Sbjct: 69  QCINCEKLQHAQQTCEDCSTLFGEYYCSICHLFDKD--KRQYHCESCGICRIGPKEDFFH 128

Query: 133 CEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMES 192
           C KC  C +T LR  H C+EN  +  CPIC E +  S     V+PCGH +H  C+ EM  
Sbjct: 129 CLKCNLCLTTNLRGKHKCIENVSRQNCPICLEDIHTSRVVAHVLPCGHLLHRTCYEEMLK 188

Query: 193 QNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSH-EVSILCNDCSHTSKVRFHILG 252
           +  YRCP+C  + +DM+  W  L  E+  TPMP EY +  V ILCNDC+  S V+FHILG
Sbjct: 189 EG-YRCPLCMHSALDMTRYWRQLDTEVAQTPMPSEYQNVTVDILCNDCNGRSTVQFHILG 248

Query: 253 HKCSQCNSFNT 263
            KC  C+S+NT
Sbjct: 249 MKCKLCDSYNT 248

[Close]
BLAST of Csor.00g266060 vs. NCBI nr
Match: XP_022953225.1 (E3 ubiquitin-protein ligase MIEL1-like [Cucurbita moschata] >KAG6575860.1 E3 ubiquitin-protein ligase MIEL1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 595 bits (1534), Expect = 2.53e-215
Identity = 273/273 (100.00%), Postives = 273/273 (100.00%), Query Frame = 0 

Query: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60
           MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD
Sbjct: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120
           RHELVRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN 273
           HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN 273

[Close]
BLAST of Csor.00g266060 vs. NCBI nr
Match: XP_023549279.1 (E3 ubiquitin-protein ligase MIEL1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 589 bits (1518), Expect = 6.96e-213
Identity = 271/273 (99.27%), Postives = 271/273 (99.27%), Query Frame = 0 

Query: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60
           MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD
Sbjct: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120
           RHELVRQDVRQVVCLICNTEQEVAKSC KCNVNMGEYFCGICKFYDDDTRK QFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEVAKSCSKCNVNMGEYFCGICKFYDDDTRKMQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN 273
           HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN 273

[Close]
BLAST of Csor.00g266060 vs. NCBI nr
Match: XP_022992470.1 (E3 ubiquitin-protein ligase MIEL1-like [Cucurbita maxima])

HSP 1 Score: 589 bits (1518), Expect = 6.96e-213
Identity = 270/273 (98.90%), Postives = 272/273 (99.63%), Query Frame = 0 

Query: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60
           MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD
Sbjct: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120
           RH+LVRQDVRQVVCLICNTEQEV+KSC KCNVNMGEYFCGICKFYDDDTRKKQFHCDACG
Sbjct: 61  RHDLVRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN 273
           HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN 273

[Close]
BLAST of Csor.00g266060 vs. NCBI nr
Match: XP_022150148.1 (E3 ubiquitin-protein ligase MIEL1 [Momordica charantia] >XP_022150149.1 E3 ubiquitin-protein ligase MIEL1 [Momordica charantia])

HSP 1 Score: 560 bits (1443), Expect = 1.70e-201
Identity = 257/269 (95.54%), Postives = 263/269 (97.77%), Query Frame = 0 

Query: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60
           ME+EDS VPQSA REDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAM+SLSNPKD
Sbjct: 1   MEKEDSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120
           RHELVRQDVRQVVCLICNTEQEVAK C KCNVNMGEYFC ICKF+DDDT+KKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNH+CVENSMKSFCPICYEFLFDSIKDTTVM CGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHL+CFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLDCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGD 269
           HTSKVRFHILGHKCSQCNSFNTRRISTGD
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGD 269

[Close]
BLAST of Csor.00g266060 vs. NCBI nr
Match: XP_023004622.1 (E3 ubiquitin-protein ligase MIEL1 [Cucurbita maxima] >XP_023513585.1 E3 ubiquitin-protein ligase MIEL1 [Cucurbita pepo subsp. pepo] >KAG6593162.1 E3 ubiquitin-protein ligase MIEL1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 554 bits (1428), Expect = 3.43e-199
Identity = 253/267 (94.76%), Postives = 257/267 (96.25%), Query Frame = 0 

Query: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60
           MEQ+D PVPQSAGREDFGK QHGCEHYRRRCRIRAPCCD IFTCRHCHNEAM+SLSNPKD
Sbjct: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120
           RHELVRQDVRQVVCLICNTEQE  + C  CNVNMGEYFC ICKFYDDDT+KKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRIST 267
           HTS VRFHILGHKCSQCNSFNTRRIST
Sbjct: 241 HTSNVRFHILGHKCSQCNSFNTRRIST 267

[Close]
BLAST of Csor.00g266060 vs. ExPASy TrEMBL
Match: A0A6J1GP21 (E3 ubiquitin-protein ligase MIEL1-like OS=Cucurbita moschata OX=3662 GN=LOC111455836 PE=4 SV=1)

HSP 1 Score: 595 bits (1534), Expect = 1.22e-215
Identity = 273/273 (100.00%), Postives = 273/273 (100.00%), Query Frame = 0 

Query: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60
           MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD
Sbjct: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120
           RHELVRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN 273
           HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN 273

[Close]
BLAST of Csor.00g266060 vs. ExPASy TrEMBL
Match: A0A6J1JVS4 (E3 ubiquitin-protein ligase MIEL1-like OS=Cucurbita maxima OX=3661 GN=LOC111488790 PE=4 SV=1)

HSP 1 Score: 589 bits (1518), Expect = 3.37e-213
Identity = 270/273 (98.90%), Postives = 272/273 (99.63%), Query Frame = 0 

Query: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60
           MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD
Sbjct: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120
           RH+LVRQDVRQVVCLICNTEQEV+KSC KCNVNMGEYFCGICKFYDDDTRKKQFHCDACG
Sbjct: 61  RHDLVRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN 273
           HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGDVNEN 273

[Close]
BLAST of Csor.00g266060 vs. ExPASy TrEMBL
Match: A0A6J1D947 (E3 ubiquitin-protein ligase MIEL1 OS=Momordica charantia OX=3673 GN=LOC111018400 PE=4 SV=1)

HSP 1 Score: 560 bits (1443), Expect = 8.25e-202
Identity = 257/269 (95.54%), Postives = 263/269 (97.77%), Query Frame = 0 

Query: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60
           ME+EDS VPQSA REDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAM+SLSNPKD
Sbjct: 1   MEKEDSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120
           RHELVRQDVRQVVCLICNTEQEVAK C KCNVNMGEYFC ICKF+DDDT+KKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNH+CVENSMKSFCPICYEFLFDSIKDTTVM CGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHL+CFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLDCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGD 269
           HTSKVRFHILGHKCSQCNSFNTRRISTGD
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGD 269

[Close]
BLAST of Csor.00g266060 vs. ExPASy TrEMBL
Match: A0A6J1KQY2 (E3 ubiquitin-protein ligase MIEL1 OS=Cucurbita maxima OX=3661 GN=LOC111497863 PE=4 SV=1)

HSP 1 Score: 554 bits (1428), Expect = 1.66e-199
Identity = 253/267 (94.76%), Postives = 257/267 (96.25%), Query Frame = 0 

Query: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60
           MEQ+D PVPQSAGREDFGK QHGCEHYRRRCRIRAPCCD IFTCRHCHNEAM+SLSNPKD
Sbjct: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120
           RHELVRQDVRQVVCLICNTEQE  + C  CNVNMGEYFC ICKFYDDDT+KKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRIST 267
           HTS VRFHILGHKCSQCNSFNTRRIST
Sbjct: 241 HTSNVRFHILGHKCSQCNSFNTRRIST 267

[Close]
BLAST of Csor.00g266060 vs. ExPASy TrEMBL
Match: A0A6J1H4Y6 (E3 ubiquitin-protein ligase MIEL1 OS=Cucurbita moschata OX=3662 GN=LOC111460617 PE=4 SV=1)

HSP 1 Score: 551 bits (1419), Expect = 3.91e-198
Identity = 251/267 (94.01%), Postives = 256/267 (95.88%), Query Frame = 0 

Query: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60
           MEQ+D PVPQSAGREDFGK QHGCEHYRRRCRIRAPCCD IFTCRHCHNEAM+SLSNP D
Sbjct: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPND 60

Query: 61  RHELVRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120
           RHELVRQDVRQVVCLICNTEQE  + C  CNVNMGEYFC ICKFYDDDT+KKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRIST 267
           HTS VRFHILGHKCS+CNSFNTRRIST
Sbjct: 241 HTSNVRFHILGHKCSRCNSFNTRRIST 267

[Close]
BLAST of Csor.00g266060 vs. TAIR 10
Match: AT3G62970.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 410.2 bits (1053), Expect = 1.3e-114
Identity = 176/263 (66.92%), Postives = 212/263 (80.61%), Query Frame = 0 

Query: 5   DSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKDRHEL 64
           DS +P+    +DFGKFQ GCEHY+RRC+IRAPCC+ IF+CRHCHN++  SL +PK+RH+L
Sbjct: 19  DSSIPRD---KDFGKFQFGCEHYKRRCKIRAPCCNLIFSCRHCHNDSANSLPDPKERHDL 78

Query: 65  VRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACGICRV 124
           VRQ+V+QVVC IC TEQEVAK C  C VNMGEYFC ICKF+DDD  K+QFHCD CGICRV
Sbjct: 79  VRQNVKQVVCSICQTEQEVAKVCSNCGVNMGEYFCDICKFFDDDISKEQFHCDDCGICRV 138

Query: 125 GGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHL 184
           GGR+ FFHC+ CG+CY   LRD H C+ENS K+ CP+CYE+LFDS+K   VM CGHT+H+
Sbjct: 139 GGRDKFFHCQNCGACYGMGLRDKHSCIENSTKNSCPVCYEYLFDSVKAAHVMKCGHTMHM 198

Query: 185 ECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSK 244
           +CF +M ++NQYRCPIC+K+++DMS SW LL  EI  T MP EY  EVSILCNDC+  SK
Sbjct: 199 DCFEQMINENQYRCPICAKSMVDMSPSWHLLDFEISATEMPVEYKFEVSILCNDCNKGSK 258

Query: 245 VRFHILGHKCSQCNSFNTRRIST 268
             FHILGHKCS C S+NTRRIST
Sbjct: 259 AMFHILGHKCSDCGSYNTRRIST 278

[Close]
BLAST of Csor.00g266060 vs. TAIR 10
Match: AT5G18650.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 379.0 bits (972), Expect = 3.2e-105
Identity = 161/254 (63.39%), Postives = 202/254 (79.53%), Query Frame = 0 

Query: 14  REDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKDRHELVRQDVRQVV 73
           R  FGK   GC+HY+RRC+IRAPCC+ +F CRHCHNE+ ++L N  DRH+LVRQDV+QV+
Sbjct: 8   RLHFGKMGFGCKHYKRRCQIRAPCCNEVFDCRHCHNESTSTLRNIYDRHDLVRQDVKQVI 67

Query: 74  CLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACGICRVGGRENFFHC 133
           C +C+TEQ  A+ C  C VNMGEYFC IC FYDDDT K+QFHCD CGICRVGGRENFFHC
Sbjct: 68  CSVCDTEQPAAQVCSNCGVNMGEYFCSICIFYDDDTEKQQFHCDDCGICRVGGRENFFHC 127

Query: 134 EKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQ 193
           +KCGSCY+  LR+NH CVENSM+  CPICYE+LFDS+KDT VM CGHT+H+EC+ EM  +
Sbjct: 128 KKCGSCYAVGLRNNHRCVENSMRHHCPICYEYLFDSLKDTNVMKCGHTMHVECYNEMIKR 187

Query: 194 NQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEY-SHEVSILCNDCSHTSKVRFHILGH 253
           +++ CPICS++V+DMS +W  L  EI+ T MP +Y   +V ILCNDC+ T++V FHI+G 
Sbjct: 188 DKFCCPICSRSVIDMSKTWQRLDEEIEATAMPSDYRDKKVWILCNDCNDTTEVHFHIIGQ 247

Query: 254 KCSQCNSFNTRRIS 267
           KC  C S+NTR I+
Sbjct: 248 KCGHCRSYNTRAIA 261

[Close]
BLAST of Csor.00g266060 vs. TAIR 10
Match: AT5G25560.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 337.8 bits (865), Expect = 8.1e-93
Identity = 145/251 (57.77%), Postives = 179/251 (71.31%), Query Frame = 0 

Query: 16  DFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLS-NPKDRHELVRQDVRQVVC 75
           D G  ++GC HYRRRC IRAPCC+ IF C HCH EA  +++ + K RH++ R  V QV+C
Sbjct: 55  DRGLMEYGCPHYRRRCCIRAPCCNEIFGCHHCHYEAKNNINVDQKQRHDIPRHQVEQVIC 114

Query: 76  LICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACGICRVGGRENFFHCE 135
           L+C TEQEV + C  C V MG+YFC +CK YDDDT KKQ+HCD CGICR+GGRENFFHC 
Sbjct: 115 LLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDGCGICRIGGRENFFHCY 174

Query: 136 KCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQN 195
           KCG CYS  L++ H CVE +M   CPIC+EFLF+S  D TV+PCGHTIH +C  EM    
Sbjct: 175 KCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPCGHTIHQKCLEEMRDHY 234

Query: 196 QYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHE-VSILCNDCSHTSKVRFHILGHK 255
           QY CP+CSK+V DMS  W    MEI  TPMPE Y +  V ILCNDC   ++V++H++  K
Sbjct: 235 QYACPLCSKSVCDMSKVWEKFDMEIAATPMPEPYQNRMVQILCNDCGKKAEVQYHVVAQK 294

Query: 256 CSQCNSFNTRR 265
           C  C S+NTR+
Sbjct: 295 CPNCKSYNTRQ 305

[Close]
BLAST of Csor.00g266060 vs. TAIR 10
Match: AT5G22920.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 334.7 bits (857), Expect = 6.8e-92
Identity = 144/267 (53.93%), Postives = 184/267 (68.91%), Query Frame = 0 

Query: 4   EDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLS-NPKDRH 63
           E+    + A   + G   +GC HYRRRC+IRAPCCD IF CRHCHNEA  SL      RH
Sbjct: 7   ENEQNQEFANLMEIGSGHYGCSHYRRRCKIRAPCCDEIFDCRHCHNEAKDSLHIEQHHRH 66

Query: 64  ELVRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACGIC 123
           EL R +V +V+C +C TEQ+V ++C  C V MG+YFC  CKF+DDD  KKQ+HCD CGIC
Sbjct: 67  ELPRHEVSKVICSLCETEQDVQQNCSNCGVCMGKYFCSKCKFFDDDLSKKQYHCDECGIC 126

Query: 124 RVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTI 183
           R GG ENFFHC++C  CYS  + D H CVE +M   CP+C+E+LFDS +D TV+ CGHT+
Sbjct: 127 RTGGEENFFHCKRCRCCYSKIMEDKHQCVEGAMHHNCPVCFEYLFDSTRDITVLRCGHTM 186

Query: 184 HLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHE-VSILCNDCSH 243
           HLEC ++M   N+Y CP+CSK++ DMS  W  L  E+   PMP+ Y ++ V ILCNDC  
Sbjct: 187 HLECTKDMGLHNRYTCPVCSKSICDMSNLWKKLDEEVAAYPMPKMYENKMVWILCNDCGS 246

Query: 244 TSKVRFHILGHKCSQCNSFNTRRISTG 269
            + VRFH++ HKCS C S+NTR+   G
Sbjct: 247 NTNVRFHLIAHKCSSCGSYNTRQTQRG 273

[Close]
BLAST of Csor.00g266060 vs. TAIR 10
Match: AT5G25560.3 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 329.7 bits (844), Expect = 2.2e-90
Identity = 145/261 (55.56%), Postives = 179/261 (68.58%), Query Frame = 0 

Query: 16  DFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLS-NPKDRHELVRQDVR---- 75
           D G  ++GC HYRRRC IRAPCC+ IF C HCH EA  +++ + K RH++ R  V     
Sbjct: 55  DRGLMEYGCPHYRRRCCIRAPCCNEIFGCHHCHYEAKNNINVDQKQRHDIPRHQVEQLTR 114

Query: 76  ------QVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACGICRV 135
                 QV+CL+C TEQEV + C  C V MG+YFC +CK YDDDT KKQ+HCD CGICR+
Sbjct: 115 PLSSSSQVICLLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDGCGICRI 174

Query: 136 GGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHL 195
           GGRENFFHC KCG CYS  L++ H CVE +M   CPIC+EFLF+S  D TV+PCGHTIH 
Sbjct: 175 GGRENFFHCYKCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPCGHTIHQ 234

Query: 196 ECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHE-VSILCNDCSHTS 255
           +C  EM    QY CP+CSK+V DMS  W    MEI  TPMPE Y +  V ILCNDC   +
Sbjct: 235 KCLEEMRDHYQYACPLCSKSVCDMSKVWEKFDMEIAATPMPEPYQNRMVQILCNDCGKKA 294

Query: 256 KVRFHILGHKCSQCNSFNTRR 265
           +V++H++  KC  C S+NTR+
Sbjct: 295 EVQYHVVAQKCPNCKSYNTRQ 315

The following BLAST results are available for this feature:
BLAST of Csor.00g266060 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8VZK04.5e-10463.39E3 ubiquitin-protein ligase MIEL1 OS=Arabidopsis thaliana OX=3702 GN=MIEL1 PE=1 ... [more]
Q9FFB69.6e-9153.93E3 ubiquitin-protein ligase RZFP34 OS=Arabidopsis thaliana OX=3702 GN=RZPF34 PE=... [more]
Q5JL963.1e-8953.96Probable E3 ubiquitin-protein ligase RZFP34 OS=Oryza sativa subsp. japonica OX=3... [more]
Q10LI13.4e-8855.29E3 ubiquitin-protein ligase SRFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SR... [more]
Q9CR501.5e-6444.62RING finger and CHY zinc finger domain-containing protein 1 OS=Mus musculus OX=1... [more]
BLAST of Csor.00g266060 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022953225.12.53e-215100.00E3 ubiquitin-protein ligase MIEL1-like [Cucurbita moschata] >KAG6575860.1 E3 ubi... [more]
XP_023549279.16.96e-21399.27E3 ubiquitin-protein ligase MIEL1-like [Cucurbita pepo subsp. pepo][more]
XP_022992470.16.96e-21398.90E3 ubiquitin-protein ligase MIEL1-like [Cucurbita maxima][more]
XP_022150148.11.70e-20195.54E3 ubiquitin-protein ligase MIEL1 [Momordica charantia] >XP_022150149.1 E3 ubiqu... [more]
XP_023004622.13.43e-19994.76E3 ubiquitin-protein ligase MIEL1 [Cucurbita maxima] >XP_023513585.1 E3 ubiquiti... [more]
BLAST of Csor.00g266060 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1GP211.22e-215100.00E3 ubiquitin-protein ligase MIEL1-like OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1JVS43.37e-21398.90E3 ubiquitin-protein ligase MIEL1-like OS=Cucurbita maxima OX=3661 GN=LOC1114887... [more]
A0A6J1D9478.25e-20295.54E3 ubiquitin-protein ligase MIEL1 OS=Momordica charantia OX=3673 GN=LOC111018400... [more]
A0A6J1KQY21.66e-19994.76E3 ubiquitin-protein ligase MIEL1 OS=Cucurbita maxima OX=3661 GN=LOC111497863 PE... [more]
A0A6J1H4Y63.91e-19894.01E3 ubiquitin-protein ligase MIEL1 OS=Cucurbita moschata OX=3662 GN=LOC111460617 ... [more]
BLAST of Csor.00g266060 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G62970.11.3e-11466.92zinc finger (C3HC4-type RING finger) family protein [more]
AT5G18650.13.2e-10563.39CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G25560.18.1e-9357.77CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G22920.16.8e-9253.93CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G25560.32.2e-9055.56CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 159..201
e-value: 7.1E-8
score: 42.2
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 159..201
e-value: 4.1E-8
score: 33.5
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 159..202
score: 12.282736
IPR039512RCHY1, zinc-ribbonPFAMPF14599zinc_ribbon_6coord: 206..263
e-value: 1.7E-23
score: 82.4
NoneNo IPR availableGENE3D2.20.28.10coord: 229..271
e-value: 1.6E-17
score: 64.4
NoneNo IPR availablePANTHERPTHR21319RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1coord: 15..265
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 158..209
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 141..210
e-value: 7.5E-11
score: 43.2
IPR008913Zinc finger, CHY-typePFAMPF05495zf-CHYcoord: 24..104
e-value: 1.1E-18
score: 67.4
IPR008913Zinc finger, CHY-typePROSITEPS51266ZF_CHYcoord: 17..92
score: 24.161512
IPR031109Ring finger and CHY zinc finger domain-containing protein, plantPANTHERPTHR21319:SF12ZINC FINGER (C3HC4-TYPE RING FINGER) FAMILY PROTEINcoord: 15..265
IPR017921Zinc finger, CTCHY-typePROSITEPS51270ZF_CTCHYcoord: 94..158
score: 32.596294
IPR037274Zinc finger, CHY-type superfamilySUPERFAMILY161219CHY zinc finger-likecoord: 20..93
IPR037275Zinc finger, CTCHY-type superfamilySUPERFAMILY161245Zinc hairpin stackcoord: 95..152

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g266060.m01Csor.00g266060.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0016746 acyltransferase activity
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding