Csor.00g259040 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g259040
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionmitogen-activated protein kinase kinase kinase NPK1
LocationCsor_Chr17: 1373164 .. 1378483 (-)
RNA-Seq ExpressionCsor.00g259040
SyntenyCsor.00g259040
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAGATTTCTTCGGATCTGTTCGTCGATCCTTGGTATTTCTAGCGCCCGACGGTGACGACGGTGGGAAGTTCGGTGGCCTCGTCGAGAGGATTGGCTCCAGCATTCGAAAATCTCGAAATGGACTGTTTTCTAAGCATTCAGTGCGGGCTTTGCCACCGGTTGCGAAAGAAGATGCGCCTTCGATTTGGTGGCGGAAGGGAGAGTTGATTGGATGCGGTGCTTTTGGTCGAGTTTATATGGGGATGAATCTCGATTCTGGAGAGCTTATTGCAATCAAACAAGTCAGTTTTTGTAGATTTAGTTTATGAAATTCTTTATATGTAATCGATTTTGAAATTTTATTTTTGATTCTTGAATTCCTGGTTGCAGGTCTCAATTGCGGCTAATAGTGCTTCAAGGGAGAAAGCGCAGGTGAGAGAACGTTTCGTAATTTGAAGTTTGCTAGATGTAGATGACTTGCAGTTGAAATTAGATAGCGATTTTTCTTTACTTTCTTGGTTCAAATTGAATTCATCGAACACACTCTCACACTTTATGTTCAACATAACTATTCAGGCTCACATACGGGAGCTGGAGGAGGAAGTGAAGCTTTTGAAGAACCTTTCTCACCCCAATATTGTGGTAAGTAAAATCTACTGGTCTTTTATTTTAGAGTTGTGGTAGCCCCTTGCTTATTTTCATTGTTGTGACTTTAGAGATATCTGGGAACTGCTAGAGAGGAATCTTCATTAAATATTCTATTGGAGTTTGTTCCTGGAGGATCAATATCATCTCTGTTAGGGAAATTTGGGTCTTTCCCTGAGTCTGTAAGTGTTGTATATTTGTACCTTTAGTCTTTTAACCTCTCTGTATTTAAATAAAGTAAACTTGATGTTGAAATTTTGCTAATGGTTGTTGATTTTTTCTAAATTAGGTTATGAAAACCTACACGAAGCAGCTATTATTGGGACTTGAGTACCTTCATAAGAACGGCATTATGCATAGGGATATAAAGGTGATAGTGAAAACCTCATTTTGAGAAGGAAGAGTTTTAACTTTTAACCTTCCTTTTTTTAAAAAAAAAAAAAAAAAAAAATTAACTGAATAATGTCTACTTGAAATAGGGTGCAAATATTCTTGTAGACAATAAGGGGTGCATTAAACTTGCTGATTTTGGTGCATCCAAGAAAGTGGTTGAGCTGGTATTGCCCATTGTTTATCTAATTTAGTTACTTAAATTGATAATTAGCATTCATAACATAATAATTAGCATCTATGCAGGCAACTATAAATGGAGCCAAATCAATGAAGGGTACTCCGTATTGGATGGCTCCTGAAGTTATTCTCCAGACCGGACATAGCTTGTAAGAATTATCATCCTATGGTGAACTTTCTTGTTGGCTTTCACTGTTTCATTCCACGCCATTGACGTGGACATATGTATTAATACATAGGAAGAATACTAATGTGTTGAAATCTAAACCATGCCATTGATGTGAATGGGAGAAATACAAATGTGCTGAAACTTATTCGGAAATAATGGATATGTAACAAAAGTAGCAGAAGAAAAACATGGAGTTTTCTTATTAAGAAACTAACATTTAGACTCTTGTTCTTCTTAATTTGACGTCTTAGCTCTGCTGATATATGGAGTGTGGGGTGTACTGTTATTGAAATGGCAACGGGGAAACCTCCATGGAGCCAACAGTATCAAGAGGTTAGCAATTGAATTTACTTTAATATGCATGTGCTTGCACTTTCTTCGAATATTTTATCGATTGAGTGAATTTTACTTTCAGGTTGCTGCTCTTTTCCACATAGGGAATACTAAGTCCCATCCACCCATTCCTGAGCATCTATCTGCTGAGGCTAAAGATTTTCTGTTAAAATGTCTACAAGAGTACTTTCAAAACTCTCGACTTTTTCTATTCACTCGATTTTTTTTCCCCTGGAAGAGCCAATTATAGCTTCATTATCAATAAGATTGATTGAGACATTTACTCTATTCTCTGTTAAATTAAACTTTATCTAATTCAAATGTGCTCACAGGGAACCAAACTCAAGGCCTACTGCATCAGACTTGCTGCAGGTATATCTGAATTATTTAAGGGTTTCTTGTCAATTGGTCAAGCCTTGTGTTTATGTGTTCTAGAGAGTAGCTCTATTCTTACCCATTTTCTTTGGATATGTAGCATCCATTTGTCTCATGTGAGTATCAAGAACCTCATGCACCAGTCCGTGCCTCTTCTTTGGTTTGTATAATTTCTCGGTAACTTTAGTTTCGTCAATGGTTTTACCATCCCATCTTAACATCATTGATATTGCAGGAATCTGGGAAGCAGATGGCTGATTCTAGGCTGAATTCTAATGATATGTAAGGACTTCAATTGATGATTGTTAAATTTCTGAGTTTAGTTGATGATTTTTGTTGCCCACTGTTAAACAGTGGACTCTAGACTGATTGTTTTTGTTGGCCATGTGCAGAACAAAGTCTATAATCCAACGTTCAACTTGTGAGGGCTTGAAGGATATTTGTGAAATGGGTAGCTTGAGGTGCTCTTCTGTATTTTCTGGGAACTATGAGGTACGGTCCAACTGGGGATCGAGCAATTTTGAGGACGACATGTGCCAGATAGATGATAAAGATCTGTTTGCTAGTTCATCGATAAAGTACAAATCTATTATATCATCAAATGATTTGAATAAGGTAAGCATTGAATCCCTGATTATGTTATTTATTGTTTTTAATAACACCAGAAGAATGAGCATCTTTTCATTTTTACCAGAGTTTCAATCCCATGTGTGAACCCACTGATGACTGGCCATGTAAATTGGATGAGAGCTCAGAGCTGAGAGGCAATTTAATGGAATTGTCTGGTATTCAAACTGGCCAGGAATCTACTAGCAAGGGGAATGACTTCACTTTTCCATGTGGTCAATCAACAGCTGAGGATGATGAAGAGGTTACAGACTCGAAGATTCAAGCCTTCCTGGATGAGAAGGTACTTCTATTCTGGAAAATGTTTCTGAGTGATGGAATTATTGTGTTCAAGCTTGAATAATGCAGCATTATATTTCTTACATTAGAATTAATATTTTTCCTGTGACATAAAAAATAGGCTTTGGAACTGAAGAAGCTACAAACACCTTTATATGAAGAGTTCCGCAGCACAGTTAATGCAGCTAATGCTTTAGGAGCAGGTGGCACTGAGAATAGCAAGAGTGTATCTAATTTTTTGAAATTGCCTCCCAAATGTGGGTCACCAAGTAAGCTGCGTGGTAAAAGACTCTCATCAATTGATGCTGGTAACTGTTCCAGCCGTCAGAGCCGTATAAAACAAGCCAGCATATTGCAAGATCGAGCTCTGCAGGAAATTCAGTCGCCTCAAATTGGGGAATGGAACAGATTGCTTCATACACAGCCAGAGTCTTGCACACCAAGGTTGTAATTTGGTGGTTTATATTTCTACTTTATTTGTGGAAAGTAAATTTAACTGTCAATTATGGACAACTTGCTTGCAGTTTCTCTGAGAGACAAAGGAAATGGAAAGAAGAGCTTGAGGAAGAACTTAAAAGGAAGCGAGGTTAGTTTGCTTTGATGTTGTGATCTCAAGTCTAGTTTACTTTGATGTTGTGATCTCAAGTCTGGTTTGCTTTGATGTTGTCATCATTTTCTCTGTCGAGTGAATTGTGTTGGTTTATGTTAGTTATCTATCACCAGTAGAATATCAAGAGATACATTCAAAAATTGATTAAACGATTTAAAAGTTCACAGGCTTTTGGAGAGAGGGCTAGAGTCTATCCCCTTTATACTAAAAGTCAAAACAAAATACACACCAACTTCCATTATTGGTAACTTCCCAAAATAACTACTTAATGAGCCTAATCAACATAGTAGGTGGTGAAATTGCCATCCTCCCTTTTGATTTCCAACATGGTATCAAAGCAGTACCAATAAAATTCTGAAGTGTTAGAACAAAGAAGTTGTGAGCCTCAAATGTGTAGTCAAAGGTGACTCAAGTGTCGAACAAAGCCAATTATCCTCAAGTAGGATCATTTCCCATATTTATATGTTTTCCTTCCTAGCATGCGTGTTAGGGCTATACGATGGGTTGGCTGGAAGAGATACATGTGTCAACTCAGTAGGTTGGGTTGAGGATTATTGAGAGGAGTAGTCCCACGTTAGCTGATTAAGAGGAAGATCATGGGTTTATAAGTAAGGAATGATCTCCATTACTACAAGGCCTTATGGGAAAACCAAAAGCAAAGCCATGAGAACTATACTCAAAGTAGACAATATCATACCATTGTGAAGGTTCGTGATTTCTAACATTGTACTAGAGTCATGGCAATAGAATTCCCAAGTGTTGAACAAAGAAGTTGTGAGCCTCAAAGGTGTAGTCAAAGGTGACTCAAGTATCGAATGAAGGGTGTACTTTGTTCGAGGGCTCCAGAGAAGGAGTTGGGCCTCAGGGGAGGTTGTTTGAGAGCTCTAGAGAAGGAGGCCTCAAGGGGAGGCTTTAAGGTGGTGTACTTTGTTCGATGGGAGGATTGTTGAGGATTATTGAGAGAAGTAGTCCCACATTGGCTAATTAAGAGGAAGATCATGAGTTTATAAGTAAAAAACACTATCTTCATTGATACAAGATCTTTTAAGAAAACCAAAAGCAAAATCATGAGAGCTTATACTCAAAGTGGACAATATCATACAATTGTAGAAGTTCGTGATTTCTAACTAGTTGTGATCATCCCTAGTTAGTTGGTACCCCATCTCAACTCGGTCTCAGGGAATGACCAATGAGTTTTTTCCCTCTTTGATGCCCATGGGTGGGGAGGGTTCACCCCATAACATAACATATTTACAATTTTGGAAGCAAGGGAGATACTACTTGGAAAACAACCAAATAAAACACATAGCTTACTAAATAAAACAACCATAGCTTACTTAGAACGCAACCAAATAAAACAAATAAAACATATTTACAATTTTGGAAGCAACGGAGATACTACTTGGAAAGCAACCAAATAAAACACACAGCTTACTAAATAAAAACAACCATAGCTTACTTTGAAAGCAACCAAATAAAACAAATAACCATAGCTTACCAAGATATTGATTCGATTTATTAGAAAAGGAACACTTACATTTGGTTATTAAATAATATGATATTCATATAAGGTTTTAGTGTTTTCTATTCATTATCTTTGCACAATTATACATTAGATTTTAGTGTTTTTTCTATACATAGCTTTGCATAATTACATACGGAAAGTGAGTGCAATGCCGTGCATATTGCAGAAATGATGCGGCGCGGAACTGTAGGAGCAATTCACTAA

mRNA sequence

ATGCAAGATTTCTTCGGATCTGTTCGTCGATCCTTGGTATTTCTAGCGCCCGACGGTGACGACGGTGGGAAGTTCGGTGGCCTCGTCGAGAGGATTGGCTCCAGCATTCGAAAATCTCGAAATGGACTGTTTTCTAAGCATTCAGTGCGGGCTTTGCCACCGGTTGCGAAAGAAGATGCGCCTTCGATTTGGTGGCGGAAGGGAGAGTTGATTGGATGCGGTGCTTTTGGTCGAGTTTATATGGGGATGAATCTCGATTCTGGAGAGCTTATTGCAATCAAACAAGTCTCAATTGCGGCTAATAGTGCTTCAAGGGAGAAAGCGCAGGCTCACATACGGGAGCTGGAGGAGGAAGTGAAGCTTTTGAAGAACCTTTCTCACCCCAATATTGTGAGATATCTGGGAACTGCTAGAGAGGAATCTTCATTAAATATTCTATTGGAGTTTGTTCCTGGAGGATCAATATCATCTCTGTTAGGGAAATTTGGGTCTTTCCCTGAGTCTGTTATGAAAACCTACACGAAGCAGCTATTATTGGGACTTGAGTACCTTCATAAGAACGGCATTATGCATAGGGATATAAAGGGTGCAAATATTCTTGTAGACAATAAGGGGTGCATTAAACTTGCTGATTTTGGTGCATCCAAGAAAGTGGTTGAGCTGGCAACTATAAATGGAGCCAAATCAATGAAGGGTACTCCGTATTGGATGGCTCCTGAAGTTATTCTCCAGACCGGACATAGCTTCTCTGCTGATATATGGAGTGTGGGGTGTACTGTTATTGAAATGGCAACGGGGAAACCTCCATGGAGCCAACAGTATCAAGAGGTTGCTGCTCTTTTCCACATAGGGAATACTAAGTCCCATCCACCCATTCCTGAGCATCTATCTGCTGAGGCTAAAGATTTTCTGTTAAAATGTCTACAAGAGGAACCAAACTCAAGGCCTACTGCATCAGACTTGCTGCAGCATCCATTTGTCTCATGTGAGTATCAAGAACCTCATGCACCAGTCCGTGCCTCTTCTTTGGAATCTGGGAAGCAGATGGCTGATTCTAGGCTGAATTCTAATGATATAACAAAGTCTATAATCCAACGTTCAACTTGTGAGGGCTTGAAGGATATTTGTGAAATGGGTAGCTTGAGGTGCTCTTCTGTATTTTCTGGGAACTATGAGGTACGGTCCAACTGGGGATCGAGCAATTTTGAGGACGACATGTGCCAGATAGATGATAAAGATCTGTTTGCTAGTTCATCGATAAAGTACAAATCTATTATATCATCAAATGATTTGAATAAGAGTTTCAATCCCATGTGTGAACCCACTGATGACTGGCCATGTAAATTGGATGAGAGCTCAGAGCTGAGAGGCAATTTAATGGAATTGTCTGGTATTCAAACTGGCCAGGAATCTACTAGCAAGGGGAATGACTTCACTTTTCCATGTGGTCAATCAACAGCTGAGGATGATGAAGAGGTTACAGACTCGAAGATTCAAGCCTTCCTGGATGAGAAGGCTTTGGAACTGAAGAAGCTACAAACACCTTTATATGAAGAGTTCCGCAGCACAGTTAATGCAGCTAATGCTTTAGGAGCAGGTGGCACTGAGAATAGCAAGAGTGTATCTAATTTTTTGAAATTGCCTCCCAAATGTGGGTCACCAAGTAAGCTGCGTGGTAAAAGACTCTCATCAATTGATGCTGGTAACTGTTCCAGCCGTCAGAGCCGTATAAAACAAGCCAGCATATTGCAAGATCGAGCTCTGCAGGAAATTCAGTCGCCTCAAATTGGGGAATGGAACAGATTGCTTCATACACAGCCAGAGTCTTGCACACCAAGTTTCTCTGAGAGACAAAGGAAATGGAAAGAAGAGCTTGAGGAAGAACTTAAAAGGAAGCGAGAAATGATGCGGCGCGGAACTGTAGGAGCAATTCACTAA

Coding sequence (CDS)

ATGCAAGATTTCTTCGGATCTGTTCGTCGATCCTTGGTATTTCTAGCGCCCGACGGTGACGACGGTGGGAAGTTCGGTGGCCTCGTCGAGAGGATTGGCTCCAGCATTCGAAAATCTCGAAATGGACTGTTTTCTAAGCATTCAGTGCGGGCTTTGCCACCGGTTGCGAAAGAAGATGCGCCTTCGATTTGGTGGCGGAAGGGAGAGTTGATTGGATGCGGTGCTTTTGGTCGAGTTTATATGGGGATGAATCTCGATTCTGGAGAGCTTATTGCAATCAAACAAGTCTCAATTGCGGCTAATAGTGCTTCAAGGGAGAAAGCGCAGGCTCACATACGGGAGCTGGAGGAGGAAGTGAAGCTTTTGAAGAACCTTTCTCACCCCAATATTGTGAGATATCTGGGAACTGCTAGAGAGGAATCTTCATTAAATATTCTATTGGAGTTTGTTCCTGGAGGATCAATATCATCTCTGTTAGGGAAATTTGGGTCTTTCCCTGAGTCTGTTATGAAAACCTACACGAAGCAGCTATTATTGGGACTTGAGTACCTTCATAAGAACGGCATTATGCATAGGGATATAAAGGGTGCAAATATTCTTGTAGACAATAAGGGGTGCATTAAACTTGCTGATTTTGGTGCATCCAAGAAAGTGGTTGAGCTGGCAACTATAAATGGAGCCAAATCAATGAAGGGTACTCCGTATTGGATGGCTCCTGAAGTTATTCTCCAGACCGGACATAGCTTCTCTGCTGATATATGGAGTGTGGGGTGTACTGTTATTGAAATGGCAACGGGGAAACCTCCATGGAGCCAACAGTATCAAGAGGTTGCTGCTCTTTTCCACATAGGGAATACTAAGTCCCATCCACCCATTCCTGAGCATCTATCTGCTGAGGCTAAAGATTTTCTGTTAAAATGTCTACAAGAGGAACCAAACTCAAGGCCTACTGCATCAGACTTGCTGCAGCATCCATTTGTCTCATGTGAGTATCAAGAACCTCATGCACCAGTCCGTGCCTCTTCTTTGGAATCTGGGAAGCAGATGGCTGATTCTAGGCTGAATTCTAATGATATAACAAAGTCTATAATCCAACGTTCAACTTGTGAGGGCTTGAAGGATATTTGTGAAATGGGTAGCTTGAGGTGCTCTTCTGTATTTTCTGGGAACTATGAGGTACGGTCCAACTGGGGATCGAGCAATTTTGAGGACGACATGTGCCAGATAGATGATAAAGATCTGTTTGCTAGTTCATCGATAAAGTACAAATCTATTATATCATCAAATGATTTGAATAAGAGTTTCAATCCCATGTGTGAACCCACTGATGACTGGCCATGTAAATTGGATGAGAGCTCAGAGCTGAGAGGCAATTTAATGGAATTGTCTGGTATTCAAACTGGCCAGGAATCTACTAGCAAGGGGAATGACTTCACTTTTCCATGTGGTCAATCAACAGCTGAGGATGATGAAGAGGTTACAGACTCGAAGATTCAAGCCTTCCTGGATGAGAAGGCTTTGGAACTGAAGAAGCTACAAACACCTTTATATGAAGAGTTCCGCAGCACAGTTAATGCAGCTAATGCTTTAGGAGCAGGTGGCACTGAGAATAGCAAGAGTGTATCTAATTTTTTGAAATTGCCTCCCAAATGTGGGTCACCAAGTAAGCTGCGTGGTAAAAGACTCTCATCAATTGATGCTGGTAACTGTTCCAGCCGTCAGAGCCGTATAAAACAAGCCAGCATATTGCAAGATCGAGCTCTGCAGGAAATTCAGTCGCCTCAAATTGGGGAATGGAACAGATTGCTTCATACACAGCCAGAGTCTTGCACACCAAGTTTCTCTGAGAGACAAAGGAAATGGAAAGAAGAGCTTGAGGAAGAACTTAAAAGGAAGCGAGAAATGATGCGGCGCGGAACTGTAGGAGCAATTCACTAA

Protein sequence

MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDAPSIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVKLLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAPVRASSLESGKQMADSRLNSNDITKSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKDLFASSSIKYKSIISSNDLNKSFNPMCEPTDDWPCKLDESSELRGNLMELSGIQTGQESTSKGNDFTFPCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKSVSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWNRLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTVGAIH
Homology
BLAST of Csor.00g259040 vs. ExPASy Swiss-Prot
Match: Q40541 (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097 GN=NPK1 PE=1 SV=1)

HSP 1 Score: 789.6 bits (2038), Expect = 2.6e-227
Identity = 422/667 (63.27%), Postives = 501/667 (75.11%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDGDD------GGKFGGLVERIGSSIRKSRNGLFSKHSVRALPP 60
           MQDF GSVRRSLVF      D      G  FGG VE++GSSIRKS  G+FSK  V ALP 
Sbjct: 1   MQDFIGSVRRSLVFKQSGDFDTGAAGVGSGFGGFVEKLGSSIRKSSIGIFSKAHVPALPS 60

Query: 61  VA---------KEDAPSIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASR 120
           ++         K+D P I WRKGE+IGCGAFGRVYMGMN+DSGEL+AIK+VSIA N ASR
Sbjct: 61  ISKAELPAKARKDDTPPIRWRKGEMIGCGAFGRVYMGMNVDSGELLAIKEVSIAMNGASR 120

Query: 121 EKAQAHIRELEEEVKLLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSF 180
           E+AQAH+RELEEEV LLKNLSHPNIVRYLGTARE  SLNILLEFVPGGSISSLLGKFGSF
Sbjct: 121 ERAQAHVRELEEEVNLLKNLSHPNIVRYLGTAREAGSLNILLEFVPGGSISSLLGKFGSF 180

Query: 181 PESVMKTYTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATIN 240
           PESV++ YTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELAT+ 
Sbjct: 181 PESVIRMYTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMT 240

Query: 241 GAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGN 300
           GAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCT+IEMATGKPPWSQQYQEVAALFHIG 
Sbjct: 241 GAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTIIEMATGKPPWSQQYQEVAALFHIGT 300

Query: 301 TKSHPPIPEHLSAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAPVRASSLES 360
           TKSHPPIPEHLSAE+KDFLLKCLQ+EP+ R +AS+LLQHPFV+ E+QE    +R+S + +
Sbjct: 301 TKSHPPIPEHLSAESKDFLLKCLQKEPHLRHSASNLLQHPFVTAEHQEARPFLRSSFMGN 360

Query: 361 GKQMADSRLNSNDITKSII--QRSTCEGLKDICEMGSLRCSSVFSGN-YEVRSNWGSSNF 420
            + MA  R+   D+  SII   R++C GLKD+C + ++RCS+V+  N     S W   N 
Sbjct: 361 PENMAAQRM---DVRTSIIPDMRASCNGLKDVCGVSAVRCSTVYPENSLGKESLWKLGNS 420

Query: 421 EDDMCQIDDKDLFASSSIKYKSIISSNDLNKSFNPMCEPTDDWPCKLDESSELRGNLMEL 480
           +DDMCQ+D+ D    +S+K  S + S    KSFNPMCEP +DWPCK DES EL  +   L
Sbjct: 421 DDDMCQMDNDDFMFGASVKCSSDLHSPANYKSFNPMCEPDNDWPCKFDESPELTKSQANL 480

Query: 481 SGIQTGQESTS-------KGNDFTFPCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTP 540
              Q   + T+       +   FTFP GQS AEDD+E+T+SKI+AFLDEKA++LKKLQTP
Sbjct: 481 HYDQATIKPTNNPIMSYKEDLAFTFPSGQSAAEDDDELTESKIRAFLDEKAMDLKKLQTP 540

Query: 541 LYEEFRSTVNAANALGAGGTENSKSVSNFLKLPPKCGSPSKLRGKRLSSIDAGNCS---- 600
           LYE F +++N ++     GT N ++V + + LPPK  SP ++  +RLS+   G C+    
Sbjct: 541 LYEGFYNSLNVSSTPSPVGTGNKENVPSNINLPPKSRSPKRMLSRRLSTAIEGACAPSPV 600

Query: 601 SRQSRIKQASILQDRALQEIQSPQIGEWNRLLHTQPESCTPSFSERQRKWKEELEEELKR 639
           +   RI     L   A+QE Q P+  EW  LL +Q E+   SFSERQR+WKEEL+EEL+R
Sbjct: 601 THSKRISNIGGLNGEAIQEAQLPRHNEWKDLLGSQREAVNSSFSERQRRWKEELDEELQR 660

BLAST of Csor.00g259040 vs. ExPASy Swiss-Prot
Match: O22042 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=ANP3 PE=1 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 1.1e-198
Identity = 389/655 (59.39%), Postives = 459/655 (70.08%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVF----LAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVA 60
           MQD  GSVRRSLVF       DG  GG   G V +I SSIR SR GLFSK     LP   
Sbjct: 1   MQDILGSVRRSLVFRSSLAGDDGTSGGGLSGFVGKINSSIRSSRIGLFSK-PPPGLPAPR 60

Query: 61  KEDAPSIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELE 120
           KE+APSI WRKGELIGCGAFGRVYMGMNLDSGEL+AIKQV IA +SAS+EK Q HIRELE
Sbjct: 61  KEEAPSIRWRKGELIGCGAFGRVYMGMNLDSGELLAIKQVLIAPSSASKEKTQGHIRELE 120

Query: 121 EEVKLLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQ 180
           EEV+LLKNLSHPNIVRYLGT RE  SLNIL+EFVPGGSISSLL KFGSFPE V+  YTKQ
Sbjct: 121 EEVQLLKNLSHPNIVRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMYTKQ 180

Query: 181 LLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYW 240
           LLLGLEYLH NGIMHRDIKGANILVDNKGCI+LADFGASKKVVELAT+NGAKSMKGTPYW
Sbjct: 181 LLLGLEYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGTPYW 240

Query: 241 MAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHL 300
           MAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWS+QYQ+ AA+ HIG TK+HPPIPE L
Sbjct: 241 MAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTKAHPPIPEDL 300

Query: 301 SAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAPVRASSLESGKQMADSRLNS 360
           S EAKDFL+KCL +EP+ R +A++LLQHPFV+ + QEP+   R S  E G  +    +N 
Sbjct: 301 SPEAKDFLMKCLHKEPSLRLSATELLQHPFVTGKRQEPYPAYRNSLTECGNPITTQGMNV 360

Query: 361 NDITKSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKDLFA 420
                S+I+RSTC GLKD+CE+GSLR S ++                DD+CQ D  DL  
Sbjct: 361 RSSINSLIRRSTCSGLKDVCELGSLRSSIIYPQKSNNSGFGWRDGDSDDLCQTDMDDLCN 420

Query: 421 SSSIKYKSIISSNDLNKSFNPMCEPTDDWPCKLDESSEL---RGNLM--ELSGIQTG--- 480
             S++   +  S DLNKSFNPMC+ TD+W CK DES ++   + NL+  + S +QTG   
Sbjct: 421 IESVRNNVLSQSTDLNKSFNPMCDSTDNWSCKFDESPKVMKSKSNLLSYQASQLQTGVPC 480

Query: 481 QESTSKGNDFTFPCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAAN 540
            E TS     TF  G S AEDD + T+ KI++FLDEKA +LK+LQTPL EEF + +N   
Sbjct: 481 DEETS----LTFAGGSSVAEDDYKGTELKIKSFLDEKAQDLKRLQTPLLEEFHNAMNPGI 540

Query: 541 ALGAGGTENSKSVSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRAL 600
             GA G  N   + N   LP    +P +L  +RLS+I     S  +S   + ++   R +
Sbjct: 541 PQGALGDTN---IYNLPNLPSISKTPKRLPSRRLSAISDAMPSPLKS--SKRTLNTSRVM 600

Query: 601 QEIQSP-QIGEWNRLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTVG 643
           Q    P Q+ E  +    +  + +  FSE +RKW+EEL EEL+R RE +R    G
Sbjct: 601 QSGTEPTQVNESTK----KGVNNSRCFSEIRRKWEEELYEELERHRENLRHAGAG 641

BLAST of Csor.00g259040 vs. ExPASy Swiss-Prot
Match: O22040 (Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3702 GN=ANP1 PE=1 SV=2)

HSP 1 Score: 609.4 bits (1570), Expect = 4.8e-173
Identity = 369/670 (55.07%), Postives = 444/670 (66.27%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDGDDGGKF-----GGLVERIGSSIRKSRNGLFSKHSVRALPPV 60
           MQDFFGSVRRSLVF     DD  +      G L ++I S IRKS+  +F K S    PP 
Sbjct: 1   MQDFFGSVRRSLVFRPSSDDDNQENQPPFPGVLADKITSCIRKSK--IFIKPSFSPPPPA 60

Query: 61  AKED-APSIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRE 120
              D AP I WRKG+LIG GAFG VYMGMNLDSGEL+A+KQV IAAN AS+EK QAHI+E
Sbjct: 61  NTVDMAPPISWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQE 120

Query: 121 LEEEVKLLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYT 180
           LEEEVKLLKNLSHPNIVRYLGT RE+ +LNILLEFVPGGSISSLL KFG FPESV++TYT
Sbjct: 121 LEEEVKLLKNLSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYT 180

Query: 181 KQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTP 240
           +QLLLGLEYLH + IMHRDIKGANILVDNKGCIKLADFGASK+V ELAT+ GAKSMKGTP
Sbjct: 181 RQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTP 240

Query: 241 YWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPE 300
           YWMAPEVILQTGHSFSADIWSVGCTVIEM TGK PWSQQY+EVAA+F IG TKSHPPIP+
Sbjct: 241 YWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPD 300

Query: 301 HLSAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAPVRASSLESGKQMADSRL 360
            LS++AKDFLLKCLQE PN RPTAS+LL+HPFV  +++E  +    S L +       ++
Sbjct: 301 TLSSDAKDFLLKCLQEVPNLRPTASELLKHPFVMGKHKESASTDLGSVLNNLSTPLPLQI 360

Query: 361 NSNDITKSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSN----FEDDMCQID 420
           N+   T      STC+ + D+C  GSL  S V         N    N     EDDMC ID
Sbjct: 361 NNTKSTPD----STCDDVGDMCNFGSLNYSLVDPVKSIQNKNLWQQNDNGGDEDDMCLID 420

Query: 421 DKDLFASSSIKYKSIISSNDLNKSFNPMCEPTDDWPCKLDESSELRGNLMELSGIQTGQE 480
           D++          ++     L KS + + + +     K DES    GN            
Sbjct: 421 DENFLTFDGEMSSTLEKDCHLKKSCDDISDMSIALKSKFDESP---GN------------ 480

Query: 481 STSKGNDFTFPCGQ-STAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEF-RSTVNAAN 540
              K +  +  C Q S +EDD+E+T+SKI+AFLDEKA +LKKLQTPLYEEF  S +  + 
Sbjct: 481 -GEKESTMSMECDQPSYSEDDDELTESKIKAFLDEKAADLKKLQTPLYEEFYNSLITFSP 540

Query: 541 ALGAGGTENSK---SVSNFLKLPPKCGSPSK--LRGKRLSSIDAGNCS-------SRQSR 600
           +       NSK   +   FLKLPPK  SPS+  L G    + DA +CS       SR+  
Sbjct: 541 SCMESNLSNSKREDTARGFLKLPPKSRSPSRGPLGGSPSRATDATSCSKSPGSGGSRELN 600

Query: 601 IKQASILQDRALQEIQSPQIGEWNRLL--HTQPESCTPSFSERQRKWKEELEEELKRKR- 644
           I       D A Q+  S ++ +W  L+    Q  S   + SE ++KWKEEL++EL+RKR 
Sbjct: 601 INNGG---DEASQDGVSARVTDWRGLVVDTKQELSQCVALSEIEKKWKEELDQELERKRQ 645

BLAST of Csor.00g259040 vs. ExPASy Swiss-Prot
Match: Q9FZ36 (Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=ANP2 PE=2 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 6.1e-168
Identity = 352/664 (53.01%), Postives = 448/664 (67.47%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDGDDGGK-----FGGLV-ERIGSSIRKSRNGLFSKHSVRALPP 60
           MQD FGSVRRSLVF +   D+  +     F  L+ ++I S IRKS   +F+K      PP
Sbjct: 1   MQDLFGSVRRSLVFRSTTDDENQENHPPPFPSLLADKITSCIRKSM--VFAKSQ---SPP 60

Query: 61  --VAKEDAPSIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHI 120
                +  P I WRKG+LIG GAFG VYMGMNLDSGEL+A+KQV I +N AS+EK QAHI
Sbjct: 61  NNSTVQIKPPIRWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASKEKTQAHI 120

Query: 121 RELEEEVKLLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKT 180
           +ELEEEVKLLKNLSHPNIVRYLGT RE+ +LNILLEFVPGGSISSLL KFG+FPESV++T
Sbjct: 121 QELEEEVKLLKNLSHPNIVRYLGTVREDETLNILLEFVPGGSISSLLEKFGAFPESVVRT 180

Query: 181 YTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKG 240
           YT QLLLGLEYLH + IMHRDIKGANILVDN+GCIKLADFGASK+V ELATI+GAKSMKG
Sbjct: 181 YTNQLLLGLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKG 240

Query: 241 TPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPI 300
           TPYWMAPEVILQTGHSFSADIWSVGCTVIEM TGK PWSQQY+E+AA+FHIG TKSHPPI
Sbjct: 241 TPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKSHPPI 300

Query: 301 PEHLSAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAPVRASSLESGKQMADS 360
           P+++S++A DFLLKCLQ+EPN RPTAS+LL+HPFV+ + +E  +    S +++      S
Sbjct: 301 PDNISSDANDFLLKCLQQEPNLRPTASELLKHPFVTGKQKESASKDLTSFMDNSCSPLPS 360

Query: 361 RLNSNDITKSIIQRSTCEGLKDICEMGSLRCS------SVFSGNYEVRSNWGSSNFED-D 420
            L   +IT    Q ST + + DIC +GSL C+      S+ + +  ++SN G  + +D D
Sbjct: 361 EL--TNITS--YQTSTSDDVGDICNLGSLTCTLAFPEKSIQNNSLCLKSNNGYDDDDDND 420

Query: 421 MCQIDDKDLFASSSIKYKSIISSNDLNKSFNPMCEPTDDWPCKLDESSELRGNLMELSGI 480
           MC IDD++    +     S+ ++ D  KS + M E +D   CK DE+S   GN       
Sbjct: 421 MCLIDDENFLTYNGETGPSLDNNTDAKKSCDTMSEISDILKCKFDENS---GN------- 480

Query: 481 QTGQESTSKGNDFTFPCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVN 540
             G+  T    +   P   S +ED+ E+T+SKI+AFLD+KA ELKKLQTPLYEEF + + 
Sbjct: 481 --GETETKVSMEVDHP---SYSEDENELTESKIKAFLDDKAAELKKLQTPLYEEFYNGMI 540

Query: 541 AANAL----GAGGTENSKSVSNFLKLPPKCGSPSKLR-GKRLSSIDAGNCSSRQSRIKQA 600
             + +         +  ++   FLKLPPK  SPS+   G+  S      C S+      +
Sbjct: 541 TCSPICMESNINNNKREEAPRGFLKLPPKSRSPSQGHIGRSPSRATDAACCSKSPESGNS 600

Query: 601 SILQDRALQEIQSPQIGEWNRLLHTQPESCTPSFSERQRKWKEELEEELKRK-REMMRRG 644
           S           +P+    +     +  S + + SE +RKWKEEL++EL+RK RE+ R+ 
Sbjct: 601 S----------GAPKNSNASAGAEQESNSQSVALSEIERKWKEELDQELERKRREITRQA 630

BLAST of Csor.00g259040 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 306.6 bits (784), Expect = 6.6e-82
Identity = 142/266 (53.38%), Postives = 198/266 (74.44%), Query Frame = 0

Query: 62  SIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVKL 121
           +I W+KG+++G G +G VY+G+N D+GEL A+KQ+ I  +  S  K +  I    +E+++
Sbjct: 167 NIKWQKGQILGRGGYGSVYLGLNKDTGELFAVKQLEI-VDINSDPKLKNMILSFSKEIEV 226

Query: 122 LKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLGL 181
           +++L H NIVRYLGT+ ++S L++ LE++PGGSISSLLGKFG+F E+V+K YTKQ+L GL
Sbjct: 227 MRSLRHDNIVRYLGTSLDQSFLSVFLEYIPGGSISSLLGKFGAFSENVIKVYTKQILQGL 286

Query: 182 EYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPEV 241
            +LH N I+HRDIKGANIL+D KG +KL+DFG SK    +  ++  KSM+GTPYWMAPEV
Sbjct: 287 SFLHANSIIHRDIKGANILIDTKGIVKLSDFGCSKSFSGI--VSQFKSMQGTPYWMAPEV 346

Query: 242 ILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEAK 301
           I QTGH  S+DIWS+GC ++EMAT +PPWS   +  A ++HI ++ S P IP H+S EA 
Sbjct: 347 IKQTGHGRSSDIWSLGCVIVEMATAQPPWSNITELAAVMYHIASSNSIPNIPSHMSQEAF 406

Query: 302 DFLLKCLQEEPNSRPTASDLLQHPFV 328
           DFL  C + +P  RP A+ LL+HPF+
Sbjct: 407 DFLNLCFKRDPKERPDANQLLKHPFI 429

BLAST of Csor.00g259040 vs. NCBI nr
Match: KAG6575002.1 (Mitogen-activated protein kinase kinase kinase NPK1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013573.1 Mitogen-activated protein kinase kinase kinase NPK1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1280 bits (3313), Expect = 0.0
Identity = 645/645 (100.00%), Postives = 645/645 (100.00%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60
           MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PSIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120
           PSIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PSIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAPVRASSLESGKQMADSRLNSNDIT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAPVRASSLESGKQMADSRLNSNDIT
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAPVRASSLESGKQMADSRLNSNDIT 360

Query: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKDLFASSSI 420
           KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKDLFASSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKDLFASSSI 420

Query: 421 KYKSIISSNDLNKSFNPMCEPTDDWPCKLDESSELRGNLMELSGIQTGQESTSKGNDFTF 480
           KYKSIISSNDLNKSFNPMCEPTDDWPCKLDESSELRGNLMELSGIQTGQESTSKGNDFTF
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWPCKLDESSELRGNLMELSGIQTGQESTSKGNDFTF 480

Query: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS 540
           PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS 540

Query: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600
           VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600

Query: 601 RLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTVGAIH 645
           RLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTVGAIH
Sbjct: 601 RLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTVGAIH 645

BLAST of Csor.00g259040 vs. NCBI nr
Match: XP_023547660.1 (mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023547661.1 mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023547662.1 mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1271 bits (3289), Expect = 0.0
Identity = 641/645 (99.38%), Postives = 642/645 (99.53%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60
           MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PSIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120
           P IWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAPVRASSLESGKQMADSRLNSNDIT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHA VRASSLESGKQMADSRLNSNDIT
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRASSLESGKQMADSRLNSNDIT 360

Query: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKDLFASSSI 420
           KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKDLFASSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKDLFASSSI 420

Query: 421 KYKSIISSNDLNKSFNPMCEPTDDWPCKLDESSELRGNLMELSGIQTGQESTSKGNDFTF 480
           KYKSIISSNDLNKSFNPMCEPTDDW CKLDESSELRGNLMELSGIQTGQESTSKGNDFTF
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWACKLDESSELRGNLMELSGIQTGQESTSKGNDFTF 480

Query: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS 540
           PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS 540

Query: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600
           VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600

Query: 601 RLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTVGAIH 645
           +LLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTVGAIH
Sbjct: 601 KLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTVGAIH 645

BLAST of Csor.00g259040 vs. NCBI nr
Match: XP_022958799.1 (mitogen-activated protein kinase kinase kinase NPK1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1261 bits (3264), Expect = 0.0
Identity = 636/645 (98.60%), Postives = 639/645 (99.07%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60
           MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PSIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120
           P IWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAPVRASSLESGKQMADSRLNSNDIT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHA VRASSLESGKQMA +RLNSNDIT
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRASSLESGKQMAGARLNSNDIT 360

Query: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKDLFASSSI 420
           KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSN EDDMCQIDDKDLFASSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNSEDDMCQIDDKDLFASSSI 420

Query: 421 KYKSIISSNDLNKSFNPMCEPTDDWPCKLDESSELRGNLMELSGIQTGQESTSKGNDFTF 480
           KYKSIISSNDLNKSFNPMCEPTDDW CKLDESSELRGNLMELSGIQTGQESTSKGNDFTF
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWACKLDESSELRGNLMELSGIQTGQESTSKGNDFTF 480

Query: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS 540
           PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS 540

Query: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600
           VSNFLKLPPKCGSPSKLRGKRLSSID GNCSSRQSR+KQASILQDRALQEIQSPQIGEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDDGNCSSRQSRVKQASILQDRALQEIQSPQIGEWN 600

Query: 601 RLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTVGAIH 645
           +LLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTVGAIH
Sbjct: 601 KLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTVGAIH 645

BLAST of Csor.00g259040 vs. NCBI nr
Match: XP_023006461.1 (mitogen-activated protein kinase kinase kinase NPK1 isoform X2 [Cucurbita maxima])

HSP 1 Score: 1259 bits (3258), Expect = 0.0
Identity = 635/645 (98.45%), Postives = 639/645 (99.07%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60
           MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PSIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120
           P IWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAPVRASSLESGKQMADSRLNSNDIT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHA VR SSLESGKQMADSRLNS DIT
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRVSSLESGKQMADSRLNSKDIT 360

Query: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKDLFASSSI 420
           KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDK+LFASSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKNLFASSSI 420

Query: 421 KYKSIISSNDLNKSFNPMCEPTDDWPCKLDESSELRGNLMELSGIQTGQESTSKGNDFTF 480
           KYKSIISSNDLNKSFNPMCEPTDDW CKLDESSELRGNLMELSGI+TGQESTSKGNDFTF
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWACKLDESSELRGNLMELSGIRTGQESTSKGNDFTF 480

Query: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS 540
           PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGA GTENSKS
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAVGTENSKS 540

Query: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600
           VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600

Query: 601 RLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTVGAIH 645
           +LLHTQPESCTP+FSERQRKWKEELEEELKRKREMMRRGTVGAIH
Sbjct: 601 KLLHTQPESCTPTFSERQRKWKEELEEELKRKREMMRRGTVGAIH 645

BLAST of Csor.00g259040 vs. NCBI nr
Match: XP_022958796.1 (mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita moschata] >XP_022958797.1 mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita moschata] >XP_022958798.1 mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1255 bits (3247), Expect = 0.0
Identity = 635/646 (98.30%), Postives = 638/646 (98.76%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60
           MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PSIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120
           P IWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAPVRASSLESGKQMADSRLNSNDIT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHA VRASSLESGKQMA +RLNSNDIT
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRASSLESGKQMAGARLNSNDIT 360

Query: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKDLFASSSI 420
           KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSN EDDMCQIDDKDLFASSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNSEDDMCQIDDKDLFASSSI 420

Query: 421 KYKSIISSNDLNKSFNPMCEPTDDWPCKLDESSELRGNLMELSGIQTGQESTSKGNDFTF 480
           KYKSIISSNDLNKSFNPMCEPTDDW CKLDESSELRGNLMELSGIQTGQESTSKGNDFTF
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWACKLDESSELRGNLMELSGIQTGQESTSKGNDFTF 480

Query: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS 540
           PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS 540

Query: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600
           VSNFLKLPPKCGSPSKLRGKRLSSID GNCSSRQSR+KQASILQDRALQEIQSPQIGEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDDGNCSSRQSRVKQASILQDRALQEIQSPQIGEWN 600

Query: 601 RLLHTQPESCTPSF-SERQRKWKEELEEELKRKREMMRRGTVGAIH 645
           +LLHTQPESCTP F SERQRKWKEELEEELKRKREMMRRGTVGAIH
Sbjct: 601 KLLHTQPESCTPRFFSERQRKWKEELEEELKRKREMMRRGTVGAIH 646

BLAST of Csor.00g259040 vs. ExPASy TrEMBL
Match: A0A6J1H661 (mitogen-activated protein kinase kinase kinase NPK1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459961 PE=4 SV=1)

HSP 1 Score: 1261 bits (3264), Expect = 0.0
Identity = 636/645 (98.60%), Postives = 639/645 (99.07%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60
           MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PSIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120
           P IWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAPVRASSLESGKQMADSRLNSNDIT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHA VRASSLESGKQMA +RLNSNDIT
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRASSLESGKQMAGARLNSNDIT 360

Query: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKDLFASSSI 420
           KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSN EDDMCQIDDKDLFASSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNSEDDMCQIDDKDLFASSSI 420

Query: 421 KYKSIISSNDLNKSFNPMCEPTDDWPCKLDESSELRGNLMELSGIQTGQESTSKGNDFTF 480
           KYKSIISSNDLNKSFNPMCEPTDDW CKLDESSELRGNLMELSGIQTGQESTSKGNDFTF
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWACKLDESSELRGNLMELSGIQTGQESTSKGNDFTF 480

Query: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS 540
           PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS 540

Query: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600
           VSNFLKLPPKCGSPSKLRGKRLSSID GNCSSRQSR+KQASILQDRALQEIQSPQIGEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDDGNCSSRQSRVKQASILQDRALQEIQSPQIGEWN 600

Query: 601 RLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTVGAIH 645
           +LLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTVGAIH
Sbjct: 601 KLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTVGAIH 645

BLAST of Csor.00g259040 vs. ExPASy TrEMBL
Match: A0A6J1KXU2 (mitogen-activated protein kinase kinase kinase NPK1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499179 PE=4 SV=1)

HSP 1 Score: 1259 bits (3258), Expect = 0.0
Identity = 635/645 (98.45%), Postives = 639/645 (99.07%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60
           MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PSIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120
           P IWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAPVRASSLESGKQMADSRLNSNDIT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHA VR SSLESGKQMADSRLNS DIT
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRVSSLESGKQMADSRLNSKDIT 360

Query: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKDLFASSSI 420
           KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDK+LFASSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKNLFASSSI 420

Query: 421 KYKSIISSNDLNKSFNPMCEPTDDWPCKLDESSELRGNLMELSGIQTGQESTSKGNDFTF 480
           KYKSIISSNDLNKSFNPMCEPTDDW CKLDESSELRGNLMELSGI+TGQESTSKGNDFTF
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWACKLDESSELRGNLMELSGIRTGQESTSKGNDFTF 480

Query: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS 540
           PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGA GTENSKS
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAVGTENSKS 540

Query: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600
           VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600

Query: 601 RLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTVGAIH 645
           +LLHTQPESCTP+FSERQRKWKEELEEELKRKREMMRRGTVGAIH
Sbjct: 601 KLLHTQPESCTPTFSERQRKWKEELEEELKRKREMMRRGTVGAIH 645

BLAST of Csor.00g259040 vs. ExPASy TrEMBL
Match: A0A6J1H434 (mitogen-activated protein kinase kinase kinase NPK1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459961 PE=4 SV=1)

HSP 1 Score: 1255 bits (3247), Expect = 0.0
Identity = 635/646 (98.30%), Postives = 638/646 (98.76%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60
           MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PSIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120
           P IWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAPVRASSLESGKQMADSRLNSNDIT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHA VRASSLESGKQMA +RLNSNDIT
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRASSLESGKQMAGARLNSNDIT 360

Query: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKDLFASSSI 420
           KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSN EDDMCQIDDKDLFASSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNSEDDMCQIDDKDLFASSSI 420

Query: 421 KYKSIISSNDLNKSFNPMCEPTDDWPCKLDESSELRGNLMELSGIQTGQESTSKGNDFTF 480
           KYKSIISSNDLNKSFNPMCEPTDDW CKLDESSELRGNLMELSGIQTGQESTSKGNDFTF
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWACKLDESSELRGNLMELSGIQTGQESTSKGNDFTF 480

Query: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS 540
           PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS 540

Query: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600
           VSNFLKLPPKCGSPSKLRGKRLSSID GNCSSRQSR+KQASILQDRALQEIQSPQIGEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDDGNCSSRQSRVKQASILQDRALQEIQSPQIGEWN 600

Query: 601 RLLHTQPESCTPSF-SERQRKWKEELEEELKRKREMMRRGTVGAIH 645
           +LLHTQPESCTP F SERQRKWKEELEEELKRKREMMRRGTVGAIH
Sbjct: 601 KLLHTQPESCTPRFFSERQRKWKEELEEELKRKREMMRRGTVGAIH 646

BLAST of Csor.00g259040 vs. ExPASy TrEMBL
Match: A0A6J1L4Z4 (mitogen-activated protein kinase kinase kinase NPK1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499179 PE=4 SV=1)

HSP 1 Score: 1254 bits (3246), Expect = 0.0
Identity = 635/646 (98.30%), Postives = 639/646 (98.92%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60
           MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PSIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120
           P IWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAPVRASSLESGKQMADSRLNSNDIT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHA VR SSLESGKQMADSRLNS DIT
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRVSSLESGKQMADSRLNSKDIT 360

Query: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKDLFASSSI 420
           KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDK+LFASSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKNLFASSSI 420

Query: 421 KYKSIISSNDLNKSFNPMCEPTDDWPCKLDESSELRGNLMELSGIQTGQESTSKGNDFTF 480
           KYKSIISSNDLNKSFNPMCEPTDDW CKLDESSELRGNLMELSGI+TGQESTSKGNDFTF
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWACKLDESSELRGNLMELSGIRTGQESTSKGNDFTF 480

Query: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS 540
           PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGA GTENSKS
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAVGTENSKS 540

Query: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600
           VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600

Query: 601 RLLHTQPESCTPSF-SERQRKWKEELEEELKRKREMMRRGTVGAIH 645
           +LLHTQPESCTP+F SERQRKWKEELEEELKRKREMMRRGTVGAIH
Sbjct: 601 KLLHTQPESCTPTFFSERQRKWKEELEEELKRKREMMRRGTVGAIH 646

BLAST of Csor.00g259040 vs. ExPASy TrEMBL
Match: A0A6J1CE75 (mitogen-activated protein kinase kinase kinase NPK1 OS=Momordica charantia OX=3673 GN=LOC111009894 PE=4 SV=1)

HSP 1 Score: 1167 bits (3018), Expect = 0.0
Identity = 585/642 (91.12%), Postives = 609/642 (94.86%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60
           MQDFFGSVRRSLVFL PDGDDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPV KEDA
Sbjct: 1   MQDFFGSVRRSLVFLTPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVMKEDA 60

Query: 61  PSIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120
           P IWWR+GELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180
           LLKNLSHPNIVRYLGT R E SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSF ADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAPVRASSLESGKQMADSRLNSNDIT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHA VRASS+ESGKQM  S LNSND+ 
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMVGSGLNSNDLA 360

Query: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKDLFASSSI 420
            S IQRSTC GLKDICEMGSLRCSSV+SGNY  +SNWGSSNFE+DMCQIDDKDL  S SI
Sbjct: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYTSQSNWGSSNFEEDMCQIDDKDLLVSPSI 420

Query: 421 KYKSIISSNDLNKSFNPMCEPTDD-WPCKLDESSELRGNLMELSGIQTGQESTSKGNDFT 480
           KYKSIISS+DLNKSFNPMCEPTDD W CKLD SSEL GNLM+LSGIQT QE+T K NDFT
Sbjct: 421 KYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMDLSGIQTSQETTCKENDFT 480

Query: 481 FPCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSK 540
           FPCGQS  EDDEEVT+SKI+AFLDEKA+ELKK+QTPLYEEFRST+NAANALGA GTENSK
Sbjct: 481 FPCGQSAVEDDEEVTESKIRAFLDEKAIELKKMQTPLYEEFRSTINAANALGAVGTENSK 540

Query: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEW 600
           SVSNFLKLPPKCGSPSKLRGKRLSSIDA NCSSRQSRIKQASIL+DRALQEIQSPQ+GEW
Sbjct: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDASNCSSRQSRIKQASILEDRALQEIQSPQLGEW 600

Query: 601 NRLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTV 641
           N+LLHTQPES TPSFSERQRKWKEELEEELKRKREMMRRG V
Sbjct: 601 NKLLHTQPESSTPSFSERQRKWKEELEEELKRKREMMRRGIV 642

BLAST of Csor.00g259040 vs. TAIR 10
Match: AT3G06030.1 (NPK1-related protein kinase 3 )

HSP 1 Score: 694.5 bits (1791), Expect = 8.0e-200
Identity = 389/655 (59.39%), Postives = 459/655 (70.08%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVF----LAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVA 60
           MQD  GSVRRSLVF       DG  GG   G V +I SSIR SR GLFSK     LP   
Sbjct: 1   MQDILGSVRRSLVFRSSLAGDDGTSGGGLSGFVGKINSSIRSSRIGLFSK-PPPGLPAPR 60

Query: 61  KEDAPSIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELE 120
           KE+APSI WRKGELIGCGAFGRVYMGMNLDSGEL+AIKQV IA +SAS+EK Q HIRELE
Sbjct: 61  KEEAPSIRWRKGELIGCGAFGRVYMGMNLDSGELLAIKQVLIAPSSASKEKTQGHIRELE 120

Query: 121 EEVKLLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQ 180
           EEV+LLKNLSHPNIVRYLGT RE  SLNIL+EFVPGGSISSLL KFGSFPE V+  YTKQ
Sbjct: 121 EEVQLLKNLSHPNIVRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMYTKQ 180

Query: 181 LLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYW 240
           LLLGLEYLH NGIMHRDIKGANILVDNKGCI+LADFGASKKVVELAT+NGAKSMKGTPYW
Sbjct: 181 LLLGLEYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGTPYW 240

Query: 241 MAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHL 300
           MAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWS+QYQ+ AA+ HIG TK+HPPIPE L
Sbjct: 241 MAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTKAHPPIPEDL 300

Query: 301 SAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAPVRASSLESGKQMADSRLNS 360
           S EAKDFL+KCL +EP+ R +A++LLQHPFV+ + QEP+   R S  E G  +    +N 
Sbjct: 301 SPEAKDFLMKCLHKEPSLRLSATELLQHPFVTGKRQEPYPAYRNSLTECGNPITTQGMNV 360

Query: 361 NDITKSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKDLFA 420
                S+I+RSTC GLKD+CE+GSLR S ++                DD+CQ D  DL  
Sbjct: 361 RSSINSLIRRSTCSGLKDVCELGSLRSSIIYPQKSNNSGFGWRDGDSDDLCQTDMDDLCN 420

Query: 421 SSSIKYKSIISSNDLNKSFNPMCEPTDDWPCKLDESSEL---RGNLM--ELSGIQTG--- 480
             S++   +  S DLNKSFNPMC+ TD+W CK DES ++   + NL+  + S +QTG   
Sbjct: 421 IESVRNNVLSQSTDLNKSFNPMCDSTDNWSCKFDESPKVMKSKSNLLSYQASQLQTGVPC 480

Query: 481 QESTSKGNDFTFPCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAAN 540
            E TS     TF  G S AEDD + T+ KI++FLDEKA +LK+LQTPL EEF + +N   
Sbjct: 481 DEETS----LTFAGGSSVAEDDYKGTELKIKSFLDEKAQDLKRLQTPLLEEFHNAMNPGI 540

Query: 541 ALGAGGTENSKSVSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRAL 600
             GA G  N   + N   LP    +P +L  +RLS+I     S  +S   + ++   R +
Sbjct: 541 PQGALGDTN---IYNLPNLPSISKTPKRLPSRRLSAISDAMPSPLKS--SKRTLNTSRVM 600

Query: 601 QEIQSP-QIGEWNRLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTVG 643
           Q    P Q+ E  +    +  + +  FSE +RKW+EEL EEL+R RE +R    G
Sbjct: 601 QSGTEPTQVNESTK----KGVNNSRCFSEIRRKWEEELYEELERHRENLRHAGAG 641

BLAST of Csor.00g259040 vs. TAIR 10
Match: AT1G09000.1 (NPK1-related protein kinase 1 )

HSP 1 Score: 609.4 bits (1570), Expect = 3.4e-174
Identity = 369/670 (55.07%), Postives = 444/670 (66.27%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDGDDGGKF-----GGLVERIGSSIRKSRNGLFSKHSVRALPPV 60
           MQDFFGSVRRSLVF     DD  +      G L ++I S IRKS+  +F K S    PP 
Sbjct: 1   MQDFFGSVRRSLVFRPSSDDDNQENQPPFPGVLADKITSCIRKSK--IFIKPSFSPPPPA 60

Query: 61  AKED-APSIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRE 120
              D AP I WRKG+LIG GAFG VYMGMNLDSGEL+A+KQV IAAN AS+EK QAHI+E
Sbjct: 61  NTVDMAPPISWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQE 120

Query: 121 LEEEVKLLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYT 180
           LEEEVKLLKNLSHPNIVRYLGT RE+ +LNILLEFVPGGSISSLL KFG FPESV++TYT
Sbjct: 121 LEEEVKLLKNLSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYT 180

Query: 181 KQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTP 240
           +QLLLGLEYLH + IMHRDIKGANILVDNKGCIKLADFGASK+V ELAT+ GAKSMKGTP
Sbjct: 181 RQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTP 240

Query: 241 YWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPE 300
           YWMAPEVILQTGHSFSADIWSVGCTVIEM TGK PWSQQY+EVAA+F IG TKSHPPIP+
Sbjct: 241 YWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPD 300

Query: 301 HLSAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAPVRASSLESGKQMADSRL 360
            LS++AKDFLLKCLQE PN RPTAS+LL+HPFV  +++E  +    S L +       ++
Sbjct: 301 TLSSDAKDFLLKCLQEVPNLRPTASELLKHPFVMGKHKESASTDLGSVLNNLSTPLPLQI 360

Query: 361 NSNDITKSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSN----FEDDMCQID 420
           N+   T      STC+ + D+C  GSL  S V         N    N     EDDMC ID
Sbjct: 361 NNTKSTPD----STCDDVGDMCNFGSLNYSLVDPVKSIQNKNLWQQNDNGGDEDDMCLID 420

Query: 421 DKDLFASSSIKYKSIISSNDLNKSFNPMCEPTDDWPCKLDESSELRGNLMELSGIQTGQE 480
           D++          ++     L KS + + + +     K DES    GN            
Sbjct: 421 DENFLTFDGEMSSTLEKDCHLKKSCDDISDMSIALKSKFDESP---GN------------ 480

Query: 481 STSKGNDFTFPCGQ-STAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEF-RSTVNAAN 540
              K +  +  C Q S +EDD+E+T+SKI+AFLDEKA +LKKLQTPLYEEF  S +  + 
Sbjct: 481 -GEKESTMSMECDQPSYSEDDDELTESKIKAFLDEKAADLKKLQTPLYEEFYNSLITFSP 540

Query: 541 ALGAGGTENSK---SVSNFLKLPPKCGSPSK--LRGKRLSSIDAGNCS-------SRQSR 600
           +       NSK   +   FLKLPPK  SPS+  L G    + DA +CS       SR+  
Sbjct: 541 SCMESNLSNSKREDTARGFLKLPPKSRSPSRGPLGGSPSRATDATSCSKSPGSGGSRELN 600

Query: 601 IKQASILQDRALQEIQSPQIGEWNRLL--HTQPESCTPSFSERQRKWKEELEEELKRKR- 644
           I       D A Q+  S ++ +W  L+    Q  S   + SE ++KWKEEL++EL+RKR 
Sbjct: 601 INNGG---DEASQDGVSARVTDWRGLVVDTKQELSQCVALSEIEKKWKEELDQELERKRQ 645

BLAST of Csor.00g259040 vs. TAIR 10
Match: AT1G54960.1 (NPK1-related protein kinase 2 )

HSP 1 Score: 570.5 bits (1469), Expect = 1.7e-162
Identity = 326/596 (54.70%), Postives = 413/596 (69.30%), Query Frame = 0

Query: 61  PSIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120
           P I WRKG+LIG GAFG VYMGMNLDSGEL+A+KQV I +N AS+EK QAHI+ELEEEVK
Sbjct: 19  PPIRWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASKEKTQAHIQELEEEVK 78

Query: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180
           LLKNLSHPNIVRYLGT RE+ +LNILLEFVPGGSISSLL KFG+FPESV++TYT QLLLG
Sbjct: 79  LLKNLSHPNIVRYLGTVREDETLNILLEFVPGGSISSLLEKFGAFPESVVRTYTNQLLLG 138

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLH + IMHRDIKGANILVDN+GCIKLADFGASK+V ELATI+GAKSMKGTPYWMAPE
Sbjct: 139 LEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKGTPYWMAPE 198

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEM TGK PWSQQY+E+AA+FHIG TKSHPPIP+++S++A
Sbjct: 199 VILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKSHPPIPDNISSDA 258

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAPVRASSLESGKQMADSRLNSNDIT 360
            DFLLKCLQ+EPN RPTAS+LL+HPFV+ + +E  +    S +++      S L   +IT
Sbjct: 259 NDFLLKCLQQEPNLRPTASELLKHPFVTGKQKESASKDLTSFMDNSCSPLPSEL--TNIT 318

Query: 361 KSIIQRSTCEGLKDICEMGSLRCS------SVFSGNYEVRSNWGSSNFED-DMCQIDDKD 420
               Q ST + + DIC +GSL C+      S+ + +  ++SN G  + +D DMC IDD++
Sbjct: 319 S--YQTSTSDDVGDICNLGSLTCTLAFPEKSIQNNSLCLKSNNGYDDDDDNDMCLIDDEN 378

Query: 421 LFASSSIKYKSIISSNDLNKSFNPMCEPTDDWPCKLDESSELRGNLMELSGIQTGQESTS 480
               +     S+ ++ D  KS + M E +D   CK DE+S   GN         G+  T 
Sbjct: 379 FLTYNGETGPSLDNNTDAKKSCDTMSEISDILKCKFDENS---GN---------GETETK 438

Query: 481 KGNDFTFPCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANAL--- 540
              +   P   S +ED+ E+T+SKI+AFLD+KA ELKKLQTPLYEEF + +   + +   
Sbjct: 439 VSMEVDHP---SYSEDENELTESKIKAFLDDKAAELKKLQTPLYEEFYNGMITCSPICME 498

Query: 541 -GAGGTENSKSVSNFLKLPPKCGSPSKLR-GKRLSSIDAGNCSSRQSRIKQASILQDRAL 600
                 +  ++   FLKLPPK  SPS+   G+  S      C S+      +S       
Sbjct: 499 SNINNNKREEAPRGFLKLPPKSRSPSQGHIGRSPSRATDAACCSKSPESGNSS------- 558

Query: 601 QEIQSPQIGEWNRLLHTQPESCTPSFSERQRKWKEELEEELKRK-REMMRRGTVGA 644
               +P+    +     +  S + + SE +RKWKEEL++EL+RK RE+ R+  +G+
Sbjct: 559 ---GAPKNSNASAGAEQESNSQSVALSEIERKWKEELDQELERKRREITRQAGMGS 585

BLAST of Csor.00g259040 vs. TAIR 10
Match: AT1G53570.1 (mitogen-activated protein kinase kinase kinase 3 )

HSP 1 Score: 288.5 bits (737), Expect = 1.3e-77
Identity = 137/264 (51.89%), Postives = 191/264 (72.35%), Query Frame = 0

Query: 65  WRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVKLLKN 124
           W+KG+ +G G FG+VY+G N + G++ AIK+V + ++  + ++    +++L +E+ LL  
Sbjct: 214 WKKGKFLGSGTFGQVYLGFNSEKGKMCAIKEVKVISDDQTSKEC---LKQLNQEINLLNQ 273

Query: 125 LSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLGLEYL 184
           L HPNIV+Y G+   E +L++ LE+V GGSI  LL  +GSF E V++ YT+Q+L GL YL
Sbjct: 274 LCHPNIVQYYGSELSEETLSVYLEYVSGGSIHKLLKDYGSFTEPVIQNYTRQILAGLAYL 333

Query: 185 HKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPEVIL- 244
           H    +HRDIKGANILVD  G IKLADFG +K V   +T+    S KG+PYWMAPEV++ 
Sbjct: 334 HGRNTVHRDIKGANILVDPNGEIKLADFGMAKHVTAFSTM---LSFKGSPYWMAPEVVMS 393

Query: 245 QTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEAKDF 304
           Q G++ + DIWS+GCT++EMAT KPPWS Q++ VAA+F IGN+K  P IP+HLS +AK+F
Sbjct: 394 QNGYTHAVDIWSLGCTILEMATSKPPWS-QFEGVAAIFKIGNSKDTPEIPDHLSNDAKNF 453

Query: 305 LLKCLQEEPNSRPTASDLLQHPFV 328
           +  CLQ  P  RPTAS LL+HPF+
Sbjct: 454 IRLCLQRNPTVRPTASQLLEHPFL 470

BLAST of Csor.00g259040 vs. TAIR 10
Match: AT1G53570.2 (mitogen-activated protein kinase kinase kinase 3 )

HSP 1 Score: 288.5 bits (737), Expect = 1.3e-77
Identity = 137/264 (51.89%), Postives = 191/264 (72.35%), Query Frame = 0

Query: 65  WRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVKLLKN 124
           W+KG+ +G G FG+VY+G N + G++ AIK+V + ++  + ++    +++L +E+ LL  
Sbjct: 214 WKKGKFLGSGTFGQVYLGFNSEKGKMCAIKEVKVISDDQTSKEC---LKQLNQEINLLNQ 273

Query: 125 LSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLGLEYL 184
           L HPNIV+Y G+   E +L++ LE+V GGSI  LL  +GSF E V++ YT+Q+L GL YL
Sbjct: 274 LCHPNIVQYYGSELSEETLSVYLEYVSGGSIHKLLKDYGSFTEPVIQNYTRQILAGLAYL 333

Query: 185 HKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPEVIL- 244
           H    +HRDIKGANILVD  G IKLADFG +K V   +T+    S KG+PYWMAPEV++ 
Sbjct: 334 HGRNTVHRDIKGANILVDPNGEIKLADFGMAKHVTAFSTM---LSFKGSPYWMAPEVVMS 393

Query: 245 QTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEAKDF 304
           Q G++ + DIWS+GCT++EMAT KPPWS Q++ VAA+F IGN+K  P IP+HLS +AK+F
Sbjct: 394 QNGYTHAVDIWSLGCTILEMATSKPPWS-QFEGVAAIFKIGNSKDTPEIPDHLSNDAKNF 453

Query: 305 LLKCLQEEPNSRPTASDLLQHPFV 328
           +  CLQ  P  RPTAS LL+HPF+
Sbjct: 454 IRLCLQRNPTVRPTASQLLEHPFL 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q405412.6e-22763.27Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097... [more]
O220421.1e-19859.39Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702... [more]
O220404.8e-17355.07Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3... [more]
Q9FZ366.1e-16853.01Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana OX=3702... [more]
Q54R826.6e-8253.38Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=... [more]
Match NameE-valueIdentityDescription
KAG6575002.10.0100.00Mitogen-activated protein kinase kinase kinase NPK1, partial [Cucurbita argyrosp... [more]
XP_023547660.10.099.38mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita pepo s... [more]
XP_022958799.10.098.60mitogen-activated protein kinase kinase kinase NPK1 isoform X2 [Cucurbita moscha... [more]
XP_023006461.10.098.45mitogen-activated protein kinase kinase kinase NPK1 isoform X2 [Cucurbita maxima... [more]
XP_022958796.10.098.30mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita moscha... [more]
Match NameE-valueIdentityDescription
A0A6J1H6610.098.60mitogen-activated protein kinase kinase kinase NPK1 isoform X2 OS=Cucurbita mosc... [more]
A0A6J1KXU20.098.45mitogen-activated protein kinase kinase kinase NPK1 isoform X2 OS=Cucurbita maxi... [more]
A0A6J1H4340.098.30mitogen-activated protein kinase kinase kinase NPK1 isoform X1 OS=Cucurbita mosc... [more]
A0A6J1L4Z40.098.30mitogen-activated protein kinase kinase kinase NPK1 isoform X1 OS=Cucurbita maxi... [more]
A0A6J1CE750.091.12mitogen-activated protein kinase kinase kinase NPK1 OS=Momordica charantia OX=36... [more]
Match NameE-valueIdentityDescription
AT3G06030.18.0e-20059.39NPK1-related protein kinase 3 [more]
AT1G09000.13.4e-17455.07NPK1-related protein kinase 1 [more]
AT1G54960.11.7e-16254.70NPK1-related protein kinase 2 [more]
AT1G53570.11.3e-7751.89mitogen-activated protein kinase kinase kinase 3 [more]
AT1G53570.21.3e-7751.89mitogen-activated protein kinase kinase kinase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 98..125
NoneNo IPR availableCOILSCoilCoilcoord: 615..638
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 65..366
e-value: 6.2E-86
score: 290.1
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 71..323
e-value: 5.3E-17
score: 59.6
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 48..554
NoneNo IPR availablePANTHERPTHR48011:SF11MAP KINASE KINASE KINASEcoord: 48..554
NoneNo IPR availableCDDcd06606STKc_MAPKKKcoord: 65..327
e-value: 9.21199E-171
score: 485.102
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 65..327
e-value: 4.0E-100
score: 348.6
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 66..327
e-value: 2.9E-73
score: 246.5
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 65..327
score: 52.244225
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 71..94
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 189..201
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 65..328

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g259040.m01Csor.00g259040.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity