Csor.00g257510 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g257510
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionSerine hydroxymethyltransferase
LocationCsor_Chr17: 480322 .. 482463 (-)
RNA-Seq ExpressionCsor.00g257510
SyntenyCsor.00g257510
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCCCGTCAATGCTTGGGGCAATACTCCTCTCGTAAACGTTGATCCTGAGATCTTTGATTTGATCGAGAAGGAGAAGCGTCGCCAATGCCGCGGAATCGAGCTCATTGCTTCTGAGAATTTCACCTCCTTCGCCGTCATCGAGGCCCTCGGAAGTGCCTTGACCAACAAATACTCCGAGGGCATGCCGGGGAACCGATACTACGGTGGAAACGAGTTCATCGATGAGATCGAGAATCTCTGCCGTTCTCGAGCTCTCGAGGCTTTCCATGTCGATCCGGCCAAATGGGGCGTTAATGTCCAGCCCTACTCTGGTTCTCCGGCGAATTTCGCCGCCTACACCGCGTTGCTTCAGCCGCATGACCGGATTATGGGGCTGGATCTTCCTTCTGGTGGCCATTTGACGCATGGTTACTACACCTCCGGAGGGAAGAAGATCTCCGCTACCTCGATTTACTTCGAGAGTTTGCCTTACAAGGTTGATTCCGCCACTGGATTTATTGATTACGATAAGTTGGAGGAGAAGGCTTTGGATTTCAGGCCGAAGTTGATTATCTGTGGTGGCAGTGCGTACCCGAGGGACTGGGATTATGCCAGATTCAGGGCCATTGCTGATAAATGCGGTGCCCTTCTTCTCTGTGATATGGCTCACATTAGTGGTCTCGTTGCTGCTCAGGTATATAATGAAACAAATCTATGAATTGAGTTCTATAACTTATCACTTTATCTTGCGAAAGTTGCCAATTGGATGTTGTTTTCCATTTCTGAATTTGGATCTGCTGGTTTATGTTGATTAGGAATAGTTAGAAATGGCACAGGTTTTCAGATCTATAATTGTTTGAGCATCTTACGCAGCAGGATCTGGCATACTAAGAAGCTTATTTAATGCCTAATTATCGATGCTACATTGCTTCTTTGTAACAGAAGATAAATTATGTTCAATGGATTGACGGAACGGTTATGTTTTCAATTGTTTCTTACAAACATTTTGCATTCCTTGTTCTTATGATTAAGTTTTAAATGCTAGATTAAAATCGAAATGAAGATCTACTTTGAGAAAATGGATAGATTTCTGTCTCGTTTTGTTTTGAACTGATATTGTGATTGCTTATGATCTCTGTTTCTTTTCTTGCAACTTAAGCGAGTTGGGTCTGGCTGGTATTTTAAAATATTGATGTCCAATGATCGTTTGCAGGAAGCTGCGAACCCGTTCGAGTACTGTGACGTTGTGACGACCACGACTCACAAGAGTTTGAGGGGTCCTAGGGCTGGTATGATCTTCTATAGGAAGGGTCCAAAGCCCCCTAAGAAGGGTCAGCCCGAGAATGCTGTCTATGACTTTGAAGACAAGATCAACTTCTCTGTCTTCCCAGCCCTTCAGGGTGGCCCTCACAATCATCAAATTGGCGCTTTAGCTGTTGCCTTGAAGCAGGCTATGTCCCCTGGGTTCAAGGTCTATGCGAAACAAGTTAAGGCCAATGCCGTGGCCCTCGGAAATTACCTGATGAACAAGGGCTACAAGCTTGTCACCGGAGGAACCGAGAACCATCTCGTTCTCTGGGATCTTCGTCCATTGGGATTGACCGGTAATTTTGTGGAATCAACCTAAGAAAACTAACAGGAAGAACATATTCTTTTTTGGTAATGTAACAATGTTGTTGTAATTCCTTTTGCAGGCAACAAGGTCGAGAAGCTTTGCGACTTGTGCAACATCACTGTAAACAAAAACGCCGTGTTTGGCGACAGCAGCGCACTGGCTCCTGGAGGTGTTAGAATCGGTAAGTTACCGATATCATAATCCACAGTCCTTAACTTGTTCTTCCTGTAATCAATCAATGAGTAGCAATTTCAAACACATGATTAACAAGACATCAAATTGTGATGGCAATCATTAGGTGCACCGGCCATGACTTCTCGAGGTTTGGTCGAGAAAGACTTCGAACAGATCGCTGAGTTCCTGCACCGTGCTGTAACCATCACCTTGAATGTCCAGAAGGAACATGGAAAGCTGTTGAAGGACTTCAACAAGGGTCTGGTAAACAACAAGGAAATCGAAGCGCTGAAGGCGGACGTCGAGAAATTTTCTGGCTCGTTCGAGATGCCCGGTTTTCTCATGTCTGAGATGAAGTACAAGGACTAG

mRNA sequence

ATGGATCCCGTCAATGCTTGGGGCAATACTCCTCTCGTAAACGTTGATCCTGAGATCTTTGATTTGATCGAGAAGGAGAAGCGTCGCCAATGCCGCGGAATCGAGCTCATTGCTTCTGAGAATTTCACCTCCTTCGCCGTCATCGAGGCCCTCGGAAGTGCCTTGACCAACAAATACTCCGAGGGCATGCCGGGGAACCGATACTACGGTGGAAACGAGTTCATCGATGAGATCGAGAATCTCTGCCGTTCTCGAGCTCTCGAGGCTTTCCATGTCGATCCGGCCAAATGGGGCGTTAATGTCCAGCCCTACTCTGGTTCTCCGGCGAATTTCGCCGCCTACACCGCGTTGCTTCAGCCGCATGACCGGATTATGGGGCTGGATCTTCCTTCTGGTGGCCATTTGACGCATGGTTACTACACCTCCGGAGGGAAGAAGATCTCCGCTACCTCGATTTACTTCGAGAGTTTGCCTTACAAGGTTGATTCCGCCACTGGATTTATTGATTACGATAAGTTGGAGGAGAAGGCTTTGGATTTCAGGCCGAAGTTGATTATCTGTGGTGGCAGTGCGTACCCGAGGGACTGGGATTATGCCAGATTCAGGGCCATTGCTGATAAATGCGGTGCCCTTCTTCTCTGTGATATGGCTCACATTAGTGGTCTCGTTGCTGCTCAGGAAGCTGCGAACCCGTTCGAGTACTGTGACGTTGTGACGACCACGACTCACAAGAGTTTGAGGGGTCCTAGGGCTGGTATGATCTTCTATAGGAAGGGTCCAAAGCCCCCTAAGAAGGGTCAGCCCGAGAATGCTGTCTATGACTTTGAAGACAAGATCAACTTCTCTGTCTTCCCAGCCCTTCAGGGTGGCCCTCACAATCATCAAATTGGCGCTTTAGCTGTTGCCTTGAAGCAGGCTATGTCCCCTGGGTTCAAGGTCTATGCGAAACAAGTTAAGGCCAATGCCGTGGCCCTCGGAAATTACCTGATGAACAAGGGCTACAAGCTTGTCACCGGAGGAACCGAGAACCATCTCGTTCTCTGGGATCTTCGTCCATTGGGATTGACCGGCAACAAGGTCGAGAAGCTTTGCGACTTGTGCAACATCACTGTAAACAAAAACGCCGTGTTTGGCGACAGCAGCGCACTGGCTCCTGGAGGTGTTAGAATCGGTGCACCGGCCATGACTTCTCGAGGTTTGGTCGAGAAAGACTTCGAACAGATCGCTGAGTTCCTGCACCGTGCTGTAACCATCACCTTGAATGTCCAGAAGGAACATGGAAAGCTGTTGAAGGACTTCAACAAGGGTCTGGTAAACAACAAGGAAATCGAAGCGCTGAAGGCGGACGTCGAGAAATTTTCTGGCTCGTTCGAGATGCCCGGTTTTCTCATGTCTGAGATGAAGTACAAGGACTAG

Coding sequence (CDS)

ATGGATCCCGTCAATGCTTGGGGCAATACTCCTCTCGTAAACGTTGATCCTGAGATCTTTGATTTGATCGAGAAGGAGAAGCGTCGCCAATGCCGCGGAATCGAGCTCATTGCTTCTGAGAATTTCACCTCCTTCGCCGTCATCGAGGCCCTCGGAAGTGCCTTGACCAACAAATACTCCGAGGGCATGCCGGGGAACCGATACTACGGTGGAAACGAGTTCATCGATGAGATCGAGAATCTCTGCCGTTCTCGAGCTCTCGAGGCTTTCCATGTCGATCCGGCCAAATGGGGCGTTAATGTCCAGCCCTACTCTGGTTCTCCGGCGAATTTCGCCGCCTACACCGCGTTGCTTCAGCCGCATGACCGGATTATGGGGCTGGATCTTCCTTCTGGTGGCCATTTGACGCATGGTTACTACACCTCCGGAGGGAAGAAGATCTCCGCTACCTCGATTTACTTCGAGAGTTTGCCTTACAAGGTTGATTCCGCCACTGGATTTATTGATTACGATAAGTTGGAGGAGAAGGCTTTGGATTTCAGGCCGAAGTTGATTATCTGTGGTGGCAGTGCGTACCCGAGGGACTGGGATTATGCCAGATTCAGGGCCATTGCTGATAAATGCGGTGCCCTTCTTCTCTGTGATATGGCTCACATTAGTGGTCTCGTTGCTGCTCAGGAAGCTGCGAACCCGTTCGAGTACTGTGACGTTGTGACGACCACGACTCACAAGAGTTTGAGGGGTCCTAGGGCTGGTATGATCTTCTATAGGAAGGGTCCAAAGCCCCCTAAGAAGGGTCAGCCCGAGAATGCTGTCTATGACTTTGAAGACAAGATCAACTTCTCTGTCTTCCCAGCCCTTCAGGGTGGCCCTCACAATCATCAAATTGGCGCTTTAGCTGTTGCCTTGAAGCAGGCTATGTCCCCTGGGTTCAAGGTCTATGCGAAACAAGTTAAGGCCAATGCCGTGGCCCTCGGAAATTACCTGATGAACAAGGGCTACAAGCTTGTCACCGGAGGAACCGAGAACCATCTCGTTCTCTGGGATCTTCGTCCATTGGGATTGACCGGCAACAAGGTCGAGAAGCTTTGCGACTTGTGCAACATCACTGTAAACAAAAACGCCGTGTTTGGCGACAGCAGCGCACTGGCTCCTGGAGGTGTTAGAATCGGTGCACCGGCCATGACTTCTCGAGGTTTGGTCGAGAAAGACTTCGAACAGATCGCTGAGTTCCTGCACCGTGCTGTAACCATCACCTTGAATGTCCAGAAGGAACATGGAAAGCTGTTGAAGGACTTCAACAAGGGTCTGGTAAACAACAAGGAAATCGAAGCGCTGAAGGCGGACGTCGAGAAATTTTCTGGCTCGTTCGAGATGCCCGGTTTTCTCATGTCTGAGATGAAGTACAAGGACTAG

Protein sequence

MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDFRPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALAVALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITLNVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD
Homology
BLAST of Csor.00g257510 vs. ExPASy Swiss-Prot
Match: O23254 (Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 SV=1)

HSP 1 Score: 904.0 bits (2335), Expect = 6.9e-262
Identity = 433/471 (91.93%), Postives = 456/471 (96.82%), Query Frame = 0

Query: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+PV++WGNT LV+VDPEI DLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MEPVSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EG+PGNRYYGGNEFIDEIENLCRSRALEAFH DPA WGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGIPGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKV+  TG+IDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKL+ICGGSAYPRDWDYARFRAIADK GALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE AVYDFEDKINF+VFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEGAVYDFEDKINFAVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQA +PGFKVYAKQVKANAVALGNYLM+KGY++VT GTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLC+IT+NKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQI EFL RAVT+TL
Sbjct: 361 EKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTLTL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 472
           ++QK +GKLLKDFNKGLVNNK+++ LKADVEKFS S+EMPGFLMSEMKYKD
Sbjct: 421 DIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPGFLMSEMKYKD 471

BLAST of Csor.00g257510 vs. ExPASy Swiss-Prot
Match: Q9SVM4 (Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 SV=1)

HSP 1 Score: 813.9 bits (2101), Expect = 9.3e-235
Identity = 382/468 (81.62%), Postives = 430/468 (91.88%), Query Frame = 0

Query: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+PV +WGNT L  VDPEI+DLIEKEK RQCRGIELIA+ENFTS AV+EALGS LTNKYS
Sbjct: 1   MEPVYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGG EFIDEIE+LCRSR+LEAFH +P KWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGH+THGYY+SGGK ISATSIYFE+LPYKVDS TG+IDYDKLEEKA+DF
Sbjct: 121 HDRIMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGG++YPR+WDYARFRA+ADK GA LLCDMAH S LVAAQEAA+PFEYCDVVTT
Sbjct: 181 RPKLIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           +THKSLRGPRAGMIFYRKGPKP KKGQPE  VYDF+ KIN +VFPALQ GPHN++IGALA
Sbjct: 241 STHKSLRGPRAGMIFYRKGPKPAKKGQPEGEVYDFDAKINSAVFPALQSGPHNNKIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQ M+P FKVYAKQVKANA  L +YL+NKGY LVT GT+NHL+LWDLRPLGLTGNKV
Sbjct: 301 VALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EK+C+LC IT+N+NAVFGD+S LAPGGVRIG PAMTSRGLVEKDFE+I EFLHRAVTITL
Sbjct: 361 EKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTITL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMK 469
           ++Q+++GK++KDFNKGLVNNKEI+ +KADVE+F+  F+MPGF +SE +
Sbjct: 421 DIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPGFFISESR 468

BLAST of Csor.00g257510 vs. ExPASy Swiss-Prot
Match: Q84WV0 (Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 3.3e-179
Identity = 295/466 (63.30%), Postives = 369/466 (79.18%), Query Frame = 0

Query: 4   VNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGM 63
           V AWG+ P+   DP+I +L+EKEK+RQ RGIELIASENF   AV+EALGS LTNKYSEGM
Sbjct: 130 VRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSHLTNKYSEGM 189

Query: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDR 123
           PG RYY GN++ID+IENLC  RAL AF ++  KWGVNVQPYS + ANFA YT LL P +R
Sbjct: 190 PGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVYTGLLLPGER 249

Query: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDFRPK 183
           IMGLD PSGGH++HGY T GGKKISA SI+FES PYKV+  TG+IDYDKLE+KALD+RPK
Sbjct: 250 IMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLEDKALDYRPK 309

Query: 184 LIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243
           ++ICGGS+YPRDWD+AR R IADKCGA+L+CDMAHISGLVA +E +NPF++CD+VT+TTH
Sbjct: 310 ILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFDHCDIVTSTTH 369

Query: 244 KSLRGPRAGMIFYRKGPKPPKKGQ-----PENAVYDFEDKINFSVFPALQGGPHNHQIGA 303
           K LRGPR G+IFYR+GPK  K+G        +  YD E+KINF+VFP+LQGGPHN+ I A
Sbjct: 370 KGLRGPRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSLQGGPHNNHIAA 429

Query: 304 LAVALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGN 363
           LA+ALKQ  +P +K Y +Q+K NA AL   L+ +  +LVTGGT+NHL+LWDL P+GLTG 
Sbjct: 430 LAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLWDLTPMGLTGK 489

Query: 364 KVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTI 423
             EK+C++C+IT+NK A+FGD+  ++PGGVRIG PAMT+RG +E DFE +A+FL +A  I
Sbjct: 490 VYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETMADFLIKAAQI 549

Query: 424 TLNVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLM 465
           T  +Q+EHGK  K+F K L  NK+I  L+  VE F+  +EMP  L+
Sbjct: 550 TSALQREHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMPASLI 595

BLAST of Csor.00g257510 vs. ExPASy Swiss-Prot
Match: Q9LM59 (Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 SV=1)

HSP 1 Score: 629.0 bits (1621), Expect = 4.3e-179
Identity = 298/466 (63.95%), Postives = 369/466 (79.18%), Query Frame = 0

Query: 4   VNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGM 63
           V AWGN  +   DPEI + +EKEK+RQ RGIELIASENF   AV+EALGS LTNKYSEGM
Sbjct: 134 VRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHLTNKYSEGM 193

Query: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDR 123
           PG RYY GN++ID+IE LC+ RAL AF ++  KWGVNVQPYS + ANFA +T LL P +R
Sbjct: 194 PGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVFTGLLMPGER 253

Query: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDFRPK 183
           IMGLD PSGGH++HGYYT GGKK+S  SI+FES PYKVD  TG+IDYDKLEEKALD+RPK
Sbjct: 254 IMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLEEKALDYRPK 313

Query: 184 LIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243
           ++ICGGS+YPRDW++ RFR IADKCGA+L+ DMA ISGLVAA+E+ NPF+YCD+VT+TTH
Sbjct: 314 ILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDYCDIVTSTTH 373

Query: 244 KSLRGPRAGMIFYRKGPKPPKKG-----QPENAVYDFEDKINFSVFPALQGGPHNHQIGA 303
           KSLRGPR G+IFY++G KP K+         N  YDFE+KINFSVFP+LQGGPHN+ I A
Sbjct: 374 KSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQGGPHNNHIAA 433

Query: 304 LAVALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGN 363
           LA+ALKQA SP +K+Y +QVK NA AL + L+++  KL+TGGT+NHL+LWDL PLGLTG 
Sbjct: 434 LAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWDLTPLGLTGK 493

Query: 364 KVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTI 423
             EK+C++C+ITVNK A+F ++  ++PGGVRIG+PAMTSRG +E +FE +A+FL+RA  I
Sbjct: 494 VYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMADFLYRAAQI 553

Query: 424 TLNVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLM 465
               Q+EHGKL K+  K + + KEI  L+  VE F+  F MP F M
Sbjct: 554 ASAAQREHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFDM 599

BLAST of Csor.00g257510 vs. ExPASy Swiss-Prot
Match: Q54Z26 (Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=shmt1 PE=1 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 1.4e-174
Identity = 301/454 (66.30%), Postives = 363/454 (79.96%), Query Frame = 0

Query: 8   GNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNR 67
           GNTPL  VD EIF+L+ +EK RQ +G+ELIASENFTS AV+EALGS  TNKY+EG PG+R
Sbjct: 6   GNTPLKEVDNEIFELMNREKDRQFKGLELIASENFTSRAVMEALGSHFTNKYAEGYPGSR 65

Query: 68  YYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDRIMGL 127
           YYGG E +DE+E LC+ RAL+AF +D +KWGVNVQPYSGSPANFA YTALL+PHDRIMGL
Sbjct: 66  YYGGTEVVDELETLCQKRALKAFRLDESKWGVNVQPYSGSPANFAVYTALLRPHDRIMGL 125

Query: 128 DLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDFRPKLIIC 187
           DLPSGGHLTHGY T   KKISA+SI+FES+PY++  A G IDY +LEE AL F+PKLII 
Sbjct: 126 DLPSGGHLTHGYQTD-KKKISASSIFFESMPYQI-GADGLIDYQRLEENALLFKPKLIIS 185

Query: 188 GGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTHKSLR 247
           G SAYPR+WDY R RAIADK GA L+CDMAH SGLVAAQ   +PF+YCDVVT+TTHK+LR
Sbjct: 186 GASAYPREWDYKRMRAIADKVGAYLMCDMAHYSGLVAAQLLDSPFDYCDVVTSTTHKTLR 245

Query: 248 GPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALAVALKQAM 307
           GPR+G+IF+R+G +    G+ E   YD E KINF+VFP+LQGGPH + I  +AVALK+A 
Sbjct: 246 GPRSGIIFFRRGKRVDGNGK-EIEEYDIESKINFAVFPSLQGGPHENVIAGVAVALKEAD 305

Query: 308 SPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLC 367
           S  FK YA QVK NA A+GN LMNKGYKLVT GT+NHL+LWDLRP  LTGNK EK  D+ 
Sbjct: 306 SQEFKEYALQVKKNAAAIGNALMNKGYKLVTNGTDNHLILWDLRPKELTGNKFEKAADIA 365

Query: 368 NITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITLNVQKEHG 427
           NITVNKNAV GD++A++PGG+RIG+ A+TSRGL E DFE+IA+FL R V+I+L +Q   G
Sbjct: 366 NITVNKNAVHGDTNAISPGGIRIGSSALTSRGLKEADFEKIADFLDRIVSISLEIQGRVG 425

Query: 428 KLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPG 462
           K L DF   +  +KE+  L+ +VE+FS  F +PG
Sbjct: 426 KKLVDFVVEINKSKELLDLRKEVEEFSSKFTLPG 456

BLAST of Csor.00g257510 vs. NCBI nr
Match: XP_022959093.1 (serine hydroxymethyltransferase 4-like [Cucurbita moschata] >KAG6574849.1 Serine hydroxymethyltransferase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 967 bits (2500), Expect = 0.0
Identity = 471/471 (100.00%), Postives = 471/471 (100.00%), Query Frame = 0

Query: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471
           NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD
Sbjct: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471

BLAST of Csor.00g257510 vs. NCBI nr
Match: XP_023548526.1 (serine hydroxymethyltransferase 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 965 bits (2495), Expect = 0.0
Identity = 470/471 (99.79%), Postives = 471/471 (100.00%), Query Frame = 0

Query: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFE+CDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEHCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471
           NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD
Sbjct: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471

BLAST of Csor.00g257510 vs. NCBI nr
Match: KAG7013425.1 (Serine hydroxymethyltransferase 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 964 bits (2491), Expect = 0.0
Identity = 469/471 (99.58%), Postives = 469/471 (99.58%), Query Frame = 0

Query: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPVN WGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNTWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFR IADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFREIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471
           NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD
Sbjct: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471

BLAST of Csor.00g257510 vs. NCBI nr
Match: XP_023006191.1 (serine hydroxymethyltransferase 4-like [Cucurbita maxima])

HSP 1 Score: 963 bits (2489), Expect = 0.0
Identity = 468/471 (99.36%), Postives = 471/471 (100.00%), Query Frame = 0

Query: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGS+LTNKYS
Sbjct: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSSLTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AFHVDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE+AVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471
           NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD
Sbjct: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471

BLAST of Csor.00g257510 vs. NCBI nr
Match: XP_038875387.1 (serine hydroxymethyltransferase 4 [Benincasa hispida])

HSP 1 Score: 946 bits (2444), Expect = 0.0
Identity = 459/471 (97.45%), Postives = 464/471 (98.51%), Query Frame = 0

Query: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+ VNAWGNTPL  VDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MESVNAWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AFHVDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDS TG+IDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE+AVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFK YAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIG PAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGTPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471
           NVQKEHGKLLKDFNKGLVNNKEIE LKADVEKFSGSF+MPGFLMSEMKYKD
Sbjct: 421 NVQKEHGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 471

BLAST of Csor.00g257510 vs. ExPASy TrEMBL
Match: A0A6J1H4Y7 (Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111460192 PE=3 SV=1)

HSP 1 Score: 967 bits (2500), Expect = 0.0
Identity = 471/471 (100.00%), Postives = 471/471 (100.00%), Query Frame = 0

Query: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471
           NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD
Sbjct: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471

BLAST of Csor.00g257510 vs. ExPASy TrEMBL
Match: A0A6J1KX34 (Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111499000 PE=3 SV=1)

HSP 1 Score: 963 bits (2489), Expect = 0.0
Identity = 468/471 (99.36%), Postives = 471/471 (100.00%), Query Frame = 0

Query: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGS+LTNKYS
Sbjct: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSSLTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AFHVDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE+AVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471
           NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD
Sbjct: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471

BLAST of Csor.00g257510 vs. ExPASy TrEMBL
Match: A0A6J1CDF6 (Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111009716 PE=3 SV=1)

HSP 1 Score: 946 bits (2444), Expect = 0.0
Identity = 458/471 (97.24%), Postives = 465/471 (98.73%), Query Frame = 0

Query: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+PVNAWGNTPL  VDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MEPVNAWGNTPLKAVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALEAFH DPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHADPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATG+IDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE+AVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFK YAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471
           N+QKE+GKLLKDFNKGLVNNK+IE LKADVEKFSGSF+MPGF MSEMKYKD
Sbjct: 421 NIQKEYGKLLKDFNKGLVNNKDIEGLKADVEKFSGSFDMPGFAMSEMKYKD 471

BLAST of Csor.00g257510 vs. ExPASy TrEMBL
Match: A0A0A0LKC8 (Serine hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G145880 PE=3 SV=1)

HSP 1 Score: 944 bits (2441), Expect = 0.0
Identity = 458/471 (97.24%), Postives = 465/471 (98.73%), Query Frame = 0

Query: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPVN WGNTPL  VDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AFH DPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATG+IDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE+AVYD+EDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAM+PGFK YAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMAPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471
           NVQKE+GKLLKDFNKGLVNNKEIE LKADVEKFSGSF+MPGFLMSEMKYKD
Sbjct: 421 NVQKEYGKLLKDFNKGLVNNKEIEKLKADVEKFSGSFDMPGFLMSEMKYKD 471

BLAST of Csor.00g257510 vs. ExPASy TrEMBL
Match: A0A6J1KLX2 (Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111494472 PE=3 SV=1)

HSP 1 Score: 940 bits (2430), Expect = 0.0
Identity = 454/471 (96.39%), Postives = 467/471 (99.15%), Query Frame = 0

Query: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPVNAWGNTPL  VDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNAWGNTPLEAVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AFHVDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKV+S+TG+IDY+KLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNSSTGYIDYEKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYA+FRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYAKFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE+AVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAM+PGFKVYAKQVKANAVALGNYLM+KGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMAPGFKVYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRA+TITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAITITL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471
           N+QKE+GKLLKDFNKGLVNNKEIE LKADVEKFS SF+MPGFLMSEMKYKD
Sbjct: 421 NIQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSASFDMPGFLMSEMKYKD 471

BLAST of Csor.00g257510 vs. TAIR 10
Match: AT4G13930.1 (serine hydroxymethyltransferase 4 )

HSP 1 Score: 904.0 bits (2335), Expect = 4.9e-263
Identity = 433/471 (91.93%), Postives = 456/471 (96.82%), Query Frame = 0

Query: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+PV++WGNT LV+VDPEI DLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MEPVSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EG+PGNRYYGGNEFIDEIENLCRSRALEAFH DPA WGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGIPGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKV+  TG+IDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKL+ICGGSAYPRDWDYARFRAIADK GALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE AVYDFEDKINF+VFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEGAVYDFEDKINFAVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQA +PGFKVYAKQVKANAVALGNYLM+KGY++VT GTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLC+IT+NKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQI EFL RAVT+TL
Sbjct: 361 EKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTLTL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 472
           ++QK +GKLLKDFNKGLVNNK+++ LKADVEKFS S+EMPGFLMSEMKYKD
Sbjct: 421 DIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPGFLMSEMKYKD 471

BLAST of Csor.00g257510 vs. TAIR 10
Match: AT4G13890.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 813.9 bits (2101), Expect = 6.6e-236
Identity = 382/468 (81.62%), Postives = 430/468 (91.88%), Query Frame = 0

Query: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+PV +WGNT L  VDPEI+DLIEKEK RQCRGIELIA+ENFTS AV+EALGS LTNKYS
Sbjct: 1   MEPVYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGG EFIDEIE+LCRSR+LEAFH +P KWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGH+THGYY+SGGK ISATSIYFE+LPYKVDS TG+IDYDKLEEKA+DF
Sbjct: 121 HDRIMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGG++YPR+WDYARFRA+ADK GA LLCDMAH S LVAAQEAA+PFEYCDVVTT
Sbjct: 181 RPKLIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           +THKSLRGPRAGMIFYRKGPKP KKGQPE  VYDF+ KIN +VFPALQ GPHN++IGALA
Sbjct: 241 STHKSLRGPRAGMIFYRKGPKPAKKGQPEGEVYDFDAKINSAVFPALQSGPHNNKIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQ M+P FKVYAKQVKANA  L +YL+NKGY LVT GT+NHL+LWDLRPLGLTGNKV
Sbjct: 301 VALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EK+C+LC IT+N+NAVFGD+S LAPGGVRIG PAMTSRGLVEKDFE+I EFLHRAVTITL
Sbjct: 361 EKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTITL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMK 469
           ++Q+++GK++KDFNKGLVNNKEI+ +KADVE+F+  F+MPGF +SE +
Sbjct: 421 DIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPGFFISESR 468

BLAST of Csor.00g257510 vs. TAIR 10
Match: AT1G36370.1 (serine hydroxymethyltransferase 7 )

HSP 1 Score: 629.4 bits (1622), Expect = 2.3e-180
Identity = 295/466 (63.30%), Postives = 369/466 (79.18%), Query Frame = 0

Query: 4   VNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGM 63
           V AWG+ P+   DP+I +L+EKEK+RQ RGIELIASENF   AV+EALGS LTNKYSEGM
Sbjct: 130 VRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSHLTNKYSEGM 189

Query: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDR 123
           PG RYY GN++ID+IENLC  RAL AF ++  KWGVNVQPYS + ANFA YT LL P +R
Sbjct: 190 PGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVYTGLLLPGER 249

Query: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDFRPK 183
           IMGLD PSGGH++HGY T GGKKISA SI+FES PYKV+  TG+IDYDKLE+KALD+RPK
Sbjct: 250 IMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLEDKALDYRPK 309

Query: 184 LIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243
           ++ICGGS+YPRDWD+AR R IADKCGA+L+CDMAHISGLVA +E +NPF++CD+VT+TTH
Sbjct: 310 ILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFDHCDIVTSTTH 369

Query: 244 KSLRGPRAGMIFYRKGPKPPKKGQ-----PENAVYDFEDKINFSVFPALQGGPHNHQIGA 303
           K LRGPR G+IFYR+GPK  K+G        +  YD E+KINF+VFP+LQGGPHN+ I A
Sbjct: 370 KGLRGPRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSLQGGPHNNHIAA 429

Query: 304 LAVALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGN 363
           LA+ALKQ  +P +K Y +Q+K NA AL   L+ +  +LVTGGT+NHL+LWDL P+GLTG 
Sbjct: 430 LAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLWDLTPMGLTGK 489

Query: 364 KVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTI 423
             EK+C++C+IT+NK A+FGD+  ++PGGVRIG PAMT+RG +E DFE +A+FL +A  I
Sbjct: 490 VYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETMADFLIKAAQI 549

Query: 424 TLNVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLM 465
           T  +Q+EHGK  K+F K L  NK+I  L+  VE F+  +EMP  L+
Sbjct: 550 TSALQREHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMPASLI 595

BLAST of Csor.00g257510 vs. TAIR 10
Match: AT1G22020.1 (serine hydroxymethyltransferase 6 )

HSP 1 Score: 629.0 bits (1621), Expect = 3.0e-180
Identity = 298/466 (63.95%), Postives = 369/466 (79.18%), Query Frame = 0

Query: 4   VNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGM 63
           V AWGN  +   DPEI + +EKEK+RQ RGIELIASENF   AV+EALGS LTNKYSEGM
Sbjct: 134 VRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHLTNKYSEGM 193

Query: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDR 123
           PG RYY GN++ID+IE LC+ RAL AF ++  KWGVNVQPYS + ANFA +T LL P +R
Sbjct: 194 PGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVFTGLLMPGER 253

Query: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDFRPK 183
           IMGLD PSGGH++HGYYT GGKK+S  SI+FES PYKVD  TG+IDYDKLEEKALD+RPK
Sbjct: 254 IMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLEEKALDYRPK 313

Query: 184 LIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243
           ++ICGGS+YPRDW++ RFR IADKCGA+L+ DMA ISGLVAA+E+ NPF+YCD+VT+TTH
Sbjct: 314 ILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDYCDIVTSTTH 373

Query: 244 KSLRGPRAGMIFYRKGPKPPKKG-----QPENAVYDFEDKINFSVFPALQGGPHNHQIGA 303
           KSLRGPR G+IFY++G KP K+         N  YDFE+KINFSVFP+LQGGPHN+ I A
Sbjct: 374 KSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQGGPHNNHIAA 433

Query: 304 LAVALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGN 363
           LA+ALKQA SP +K+Y +QVK NA AL + L+++  KL+TGGT+NHL+LWDL PLGLTG 
Sbjct: 434 LAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWDLTPLGLTGK 493

Query: 364 KVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTI 423
             EK+C++C+ITVNK A+F ++  ++PGGVRIG+PAMTSRG +E +FE +A+FL+RA  I
Sbjct: 494 VYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMADFLYRAAQI 553

Query: 424 TLNVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLM 465
               Q+EHGKL K+  K + + KEI  L+  VE F+  F MP F M
Sbjct: 554 ASAAQREHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFDM 599

BLAST of Csor.00g257510 vs. TAIR 10
Match: AT4G32520.1 (serine hydroxymethyltransferase 3 )

HSP 1 Score: 563.5 bits (1451), Expect = 1.6e-160
Identity = 279/456 (61.18%), Postives = 343/456 (75.22%), Query Frame = 0

Query: 12  LVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGG 71
           L  VDPE+  +I KEK RQ R +ELIASENFTS AV+EA+GS LTNKYSEG+PG RYYGG
Sbjct: 83  LGEVDPEVRTIITKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGG 142

Query: 72  NEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDRIMGLDLPS 131
           NE+ID++E LC++RAL AF +D  KWGVNVQP SGSPANFA YTA+L PHDRIMGLDLP 
Sbjct: 143 NEYIDQLETLCQNRALAAFRLDSTKWGVNVQPLSGSPANFAVYTAILSPHDRIMGLDLPH 202

Query: 132 GGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDFRPKLIICGGSA 191
           GGHL+HG+ T+  +++S TSIYFES+PY++D +TG +DYD LE+ A  FRPKLII G SA
Sbjct: 203 GGHLSHGFMTA-KRRVSGTSIYFESMPYRLDESTGIVDYDMLEKTATLFRPKLIIAGASA 262

Query: 192 YPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTHKSLRGPRA 251
           Y RD+DY R R IAD  GA L+ DMAHISGLVAA   A+PFEYCD+VTTTTHKSLRGPR 
Sbjct: 263 YSRDFDYPRMRKIADSVGAFLMMDMAHISGLVAASVVADPFEYCDIVTTTTHKSLRGPRG 322

Query: 252 GMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALAVALKQAMSPGF 311
           GMIF+RK         P N V D E  +N +VFP LQGGPHNH IG LAV LK A SP F
Sbjct: 323 GMIFFRK--------DPINGV-DLESAVNNAVFPGLQGGPHNHTIGGLAVCLKHAQSPEF 382

Query: 312 KVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITV 371
           K Y K+V +N  AL N L+  G+KLV+GG++NHLVL DLRP+G+ G +VEK+ D+ +IT+
Sbjct: 383 KAYQKRVVSNCRALANRLVELGFKLVSGGSDNHLVLVDLRPMGMDGARVEKILDMASITL 442

Query: 372 NKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITLNVQK-EHGKLL 431
           NKN+V GD SAL PGG+RIG+PAMT+RGL EKDF  +A+F+   V IT+  +K   G  L
Sbjct: 443 NKNSVPGDKSALVPGGIRIGSPAMTTRGLSEKDFVVVADFIKEGVEITMEAKKAAPGSKL 502

Query: 432 KDFNKGLVN-----NKEIEALKADVEKFSGSFEMPG 462
           +DFNK + +      + +++LK  VE F+  F +PG
Sbjct: 503 QDFNKFVTSPEFPLKERVKSLKERVETFTSRFPIPG 528

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O232546.9e-26291.93Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 S... [more]
Q9SVM49.3e-23581.62Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 S... [more]
Q84WV03.3e-17963.30Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 S... [more]
Q9LM594.3e-17963.95Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 S... [more]
Q54Z261.4e-17466.30Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=shmt1 ... [more]
Match NameE-valueIdentityDescription
XP_022959093.10.0100.00serine hydroxymethyltransferase 4-like [Cucurbita moschata] >KAG6574849.1 Serine... [more]
XP_023548526.10.099.79serine hydroxymethyltransferase 4-like [Cucurbita pepo subsp. pepo][more]
KAG7013425.10.099.58Serine hydroxymethyltransferase 4 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023006191.10.099.36serine hydroxymethyltransferase 4-like [Cucurbita maxima][more]
XP_038875387.10.097.45serine hydroxymethyltransferase 4 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1H4Y70.0100.00Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111460192 PE... [more]
A0A6J1KX340.099.36Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111499000 PE=3... [more]
A0A6J1CDF60.097.24Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111009716 P... [more]
A0A0A0LKC80.097.24Serine hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G145880 PE=3 ... [more]
A0A6J1KLX20.096.39Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111494472 PE=3... [more]
Match NameE-valueIdentityDescription
AT4G13930.14.9e-26391.93serine hydroxymethyltransferase 4 [more]
AT4G13890.16.6e-23681.62Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT1G36370.12.3e-18063.30serine hydroxymethyltransferase 7 [more]
AT1G22020.13.0e-18063.95serine hydroxymethyltransferase 6 [more]
AT4G32520.11.6e-16061.18serine hydroxymethyltransferase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR039429Serine hydroxymethyltransferase-like domainPFAMPF00464SHMTcoord: 13..411
e-value: 8.3E-192
score: 637.1
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 41..316
e-value: 6.0E-215
score: 715.7
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 16..460
e-value: 6.0E-215
score: 715.7
IPR001085Serine hydroxymethyltransferasePIRSFPIRSF000412SHMTcoord: 1..466
e-value: 1.3E-188
score: 624.1
IPR001085Serine hydroxymethyltransferasePANTHERPTHR11680SERINE HYDROXYMETHYLTRANSFERASEcoord: 1..470
IPR001085Serine hydroxymethyltransferaseHAMAPMF_00051SHMTcoord: 11..461
score: 42.730988
IPR001085Serine hydroxymethyltransferaseCDDcd00378SHMTcoord: 15..453
e-value: 0.0
score: 615.366
NoneNo IPR availablePANTHERPTHR11680:SF33SERINE HYDROXYMETHYLTRANSFERASEcoord: 1..470
IPR019798Serine hydroxymethyltransferase, pyridoxal phosphate binding sitePROSITEPS00096SHMTcoord: 236..252
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 4..463

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g257510.m01Csor.00g257510.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046655 folic acid metabolic process
biological_process GO:0019264 glycine biosynthetic process from serine
biological_process GO:0006565 L-serine catabolic process
biological_process GO:0032259 methylation
biological_process GO:0035999 tetrahydrofolate interconversion
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004372 glycine hydroxymethyltransferase activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0070905 serine binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003824 catalytic activity