Csor.00g257440 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g257440
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionMethionyl-tRNA synthetase
LocationCsor_Chr17: 428895 .. 436634 (-)
RNA-Seq ExpressionCsor.00g257440
SyntenyCsor.00g257440
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGACGTCTTAGGCGACGCCGGTGGCGGCGGAACCAAGGCCACAAAGCTTCCGATTCCAGGAAAGAGGAATATACTCATTACCAGCGCTCTTCCATACGTCAATAACGTTCCTCACCTCGGCAACATCATTGGCTGTAATGTTCTTTATCACATGTGTATATATGTTTCCGTGTGTTTGATTGATTGAGGACTGTGTTATTTCTTTGCGTCTCTGTGCTCTTGTGTTTGAGTTGGTTGCTGTTCGGTTTCTGAGAATTGAGTGATGGAGATTTGAGGAGTTATGCCGCCTTCTCAGTTTTACTTCTAATGCGCGCGTTTTTTGTGTATATATGTTTCCGTGTTTTGATTAATTGAGGACTGTGTAATTTCTTTGCGTTTCTGTGCTCTTGTGTTTGAATTGGTTGCTGTTCGGTTTCCGAGAATTGAATGATGGAGATTTAAGAAGTTATACCGCCTTCTCAGTTTTACTTCTACTGCGCGCGCTTTTTGTGTACATATGTTTCCGTGTGTTTGAATGATTGAGGACTGTATTGTTTCTTTGCGTTTCTGTGCTCTTGTGATTGAGTTGGTTGCTGTTCGGTTTCTGATAATTGAATGATGGAGATTTGAGGAGTTATGCCGCCTTCTCAGTTTTACTTCTAATGCGCGCGCTTTTTGTGTATATATGTTTCCGTGTGTTCGATTGATTGAAAACTGTGTTGTTTCTTCGCGTTTCTGTTCTCTTGTGTTTGAGTTGGTTGCTGTTTGGTGTCTGAGAATTGAATGATGGAGATTTGAGGAGTTATGCCGCCTTCTCAGTTTTACTTCTAATGCGCGCGCTTTTTGTGTATATATGTTTCCGTGTGTTTGATTGATTGAGGACTGTGTTGTTTCTTTGCGTTTCTGTGCTCTTGTGTTTGAATTGGTTGCTGTTCGGTTTCTGAGAAATGAATTATGGAGATTTGAGGAGTTATGCCGCCTACTCTGTTTTACTTCTAATGCGCGCGCTTTTTGTGATGCGGTTATGATTTTTGGAAGGCTGGATGAAATGTTCTGTTTTATATTTGATTTCAATATCCTGTTATACTTCCACAAGCGGCTGGATTGATTTTATTTGTATGCAGGTGTGTTGAGTGCTGATGTTTTTGCTCGGTACTGTCGTCTTAGAGGTTATAACGCTATTTATATATGCGGAACCGATGAGTATGGAACGGCGACGGAGACAAAAGCAATGGAAGAGAAATGTTCTCCTAAAGAGATTTGTGACAAGTAAGATTTCCTCTCATATTTCTGTTCGTCATGGCTACTACCTTCTTCTATGATTCTGGAAAATAGTTCGCTCGGCTTTCTATAGTTTGTTTTATATATAATTTGTACTCGCGTAATGTTACACTTTCAAGTTCGCTTCTATCCTCCACGAGCATAGTTTTGTAAATCAGACAGAGATCTGGATCTCAGGCTTTTTAGTACTGTCTTCTGGACTTGGATACTCTGTCATATTCACTTCATGACATTTTTTTCATATTCAAATTGTTTTGAACGGTTCTGATTTTCACATGCAAGTAATTTCGTGGAGCATTTTTGAAGACGATCTCGTCCTTCCGTAATAGTTTAACTTGGCCGAGTTACAAGTACTTGAACACTTAATCGACTGTTCAGAATGAAGTTATATATTTTGTGACCTGCAATCTCTCTTCCTCCCTTGAATCTCAGTTTTCACTTCCTGCTTAGAGATCACCTAAATCTGAGGAAAGATTTGGACCTTTACTTAACTATTTCTGCACTGTCAGGTACCACAAAATTCACAAAGAAGTTTACGATTGGTTTGGTATTAGTTTTGATGAATTTGGTCGCACATCATCATCACAGCAGACTGAAGTTTGTCAAGAAATATTTGGAAAACTATTGGAGAACAATTGGCTTTCTGAGAACACGATGCTACAGGTAGACTTATTTATCACTTGAGTATTATATTCTTCTCCTGGTTACTTTTGGTTGCACGCGCTTGAGTTCTTCTACTTCTGTTCATTGATTTCATGCATGAAATTTATTTGTTTTTTTACTCATAATTCAGCTGTACTGTGATACTTGTGAGAGATTTTTAGCCGATCGGCTCGTTGAAGGCATCTGTCCTACTCCAGGTTGTGAGTATGACTCTGCACGTGGTGATCAATGTGAAAAGTGCGGGAAACTTCTTAATCCAACGGAGTTGAAAGATCCCAGGTGCAAGGTACTTCATGGATTTGCTGCGACTGAGAAACAATTTTTGTATTACAAAACCTTCATTAAGTGCAAGGAACTGCATAAAGTTTCCTAGGAAATTAGGAAAACACGTTCCAATTGGAAGAGTTTTTTTTTCCTTCTTTTGGCATGTTCCTTTTCACACACAGCAGCAAAGAGGTCCCTTGGCGGCCTCTTCTTTCTCTTTTTCTCTTTTTCAATTTTTTACTATTAATAATTATTGGAGAGAAATCCTGTTGAGTTTTCATATCTGCAACTCCGTTCATCCAGTTATTATAAGGAATCTATTATGGAAAAGGAAGTTTGGAAAAGCTATTGAAATCTGTTTTGCACGAAGTCCCAAGAGTCTAGGGGTCAAAGTCTCTCTTCCTTATTGGAGGACGAATTCACAGCTTGAAGCAAACAAGGAAAAGATAGTTCATAATCTATTGTTGGCAGTTTGAAGCGTGGTTTATGTTTAAGTAACACATTTGCATGGTCAAGAGTCATGAACTTTGCTTGAATGAATTTGTGTTAGATACCATCTGTGGGTACCAATTTTAACTTTTTTCAAACAGAGAGAGAGAGAGAGAGATGAATCCATCACTTCTTCTGAGGGTCTTTATATCCCTTGGAAGATCCTAGCATTTCATTCATCCCACAGTTTCCAAATGACAGCTCTAGAAAACCAGTGTTACATGAGTAGAGGTAATTAAGCAAGTTTCCAAATAACAATAGTATTTCACGTACTTTTTTCCAGGTTTATTATTAAGAAAATAGTCATAAATAAAACAAAATGATCAGTTTGTTTCATTTTTCCCCCAAGATAGCAACTGAGTGTTAATATTTGGAAAATAGGTCTGTCAAACATCCCCACGTATCCGTGACACAAACCACATGTTTCTTGAACTTCCTTTGCTGAGAGATAAACTTGAAGAATATATCAACAAGATGTCTGTGGCTGGATCTTGGAGCCAAAATGCTATTCAAGCAACACAAGCTTGGCTTAAGGAAGGGTTGAAACCCCGGTGTATTACAAGAGATCTAAAGTGGGGGGTTCCCGTTCCACTTGAAAGGTTCAAGGACAAGGTTCGTTTTTTTTTTCGATCTTTTTTATTTTAATAGCTAGACAATTTTCTAAATAAAATTTTGTTAATTTTGATTTCAATTTTTTTAGGTCTTCTATGTTTGGTTTGATGCACCAATAGGATATGTCTCCATTACATCATGCTACACAAATGAATGGGAGAAATGGTGGAAGAATCCAGAGAATGTGGAGCTCTTTCAATTCATGGGCAAGGATAATGTTCCTTTCCACACTGTAGGTATCCTTACCATTAAACTTTCCTTATTGTCTGAAGAAAATCTGTTCCTTTTTTCATATGATTTACAAACGCCATTTTCATCCTAACTTCTGGGAATAGATGACAAACGTGTTATTTTATCTGAAAATAGTTTTCTTAAAATTGAAAATGTAATTTATATGCATTTGGTAACTCTCTATATCAAGTAGTGCAAAAATTGGTTAATCTCTTCAAAGTGAGGTTTATTAGTTGTAAGATACCCCTTTCTCGATCAGTCAAAGTCAGAAACTGGAAGTTCCTTGTTTCTTACAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAATTAAAAAGACGGGAAGTTCCAGTTTCTGTTATGACCTTCTGATGAACCACTTCAAACTTGTAATTTAGCATGAATATTTCTGCAGAAGTATGGAGATATTACTTATTAGCCAATAGGCCTGAGGTAAATTTTCCACTTTATTGCCTCTGGTATTATCTGCTCTGCTCATAGCTTATATATATATTTAAAATTTTAAAATCTGTAAAATTAGGTTTCAGATACATTATTTACATGGGCTGATTTACAAGCAAAACTCAATGGCGAGTTGTTGAACAATTTGGGCAACTTCATTCATCGAGTTTTGAGCTTCATTGCAAAACCTTTAGGTTGGTACTTCTGTTTTTTCTTGTAACTTCTGCTTCAGAACCGAAATTTTCTTCAAGTGAAACTTTTAGGTATATAACTGCAACTGCAAGGTTAGAAACAGTTGCCCAATCGAGAATTTGTGCTTCTTATTCTAAAGAATCCATCATTTCAAGTGGGCACCATCTTTACTGCTATAAAGGTCTATGAAATATTTACGAGCACTATATTAATTCCTATAAAACGGCAATGAAATGTCCCAAAACTTAATTATGTTTTTGCGTCAAGAGGTAATGCAGTTTCCTACGGTTAAAACATATTTGAATCCTCGATTCACAGGGCAGGGTTATGGTTCCATAATTCCTGATGCACCTAACGCGGACTCTCATGGATTAACATTGGGTTTGGCTGAAAAAGTTGGTAAATACGTAGACCAATATGTGGAATCAATGGAGAAGGTACTTTAAAAAAAAATTGATCTGTGTTTGATTAGCCTTTTTGTTATAGGAACAGGATAATTTGTGATATTAAAATATTGACCGCTTCCTCTTTGATTTTGTCGGTTATTGAAATGAAATTGTTCTATGGAATATTTTTTACAGGTTAAACTAAAACAAGGTCTAAAAGCTGCAATGGCTATTTCAAGCGAGGGAAATACATATTTGCAAGTAAACATAGTCATTGGCTCATTTGAATCTTGTTTGTATCATGTTCATTATTGTTTTAAAATTTAAAATTTCTTTGGAATCTGGCTATTTATCATGTTTTCCATGCCAGACATCCCAGTTCTGGAATCTTTACAAGGCGGACAAACCTTCGTGCAATATTGTTATGAGAACTTCAACTGGACTTGTGCATCTTCTGGCATGTTTATTAGAACCTTTCATGCCTTCTTTCTCAGATGAGGTAAATATTCTGACGACACATTCTCCACATTTCTATTAGCAGTTGAGCGTTAATATATGGGCTGTGTTGATTTTCAGACCATTCTTTAAACAGTTGATTGATTATGGCGTCCATTATCAATCCTAATTCAAATAACTGTTTTACCAACAACTATTGTTAAAAAATCTCTACCTTTTGGAAGGAAACTTATTGTTTAATTATCACATCTCAGGTTTTTTCTTAGACAACAATAGCTTATAAATAGCTTATAATATATGGTTAAGAATCACATAGATATAATATTGTCCACTTTTAGCATCAACTTCATGACTTTGCTTTTAGTTTCTCCAAAAGGTTTCATACGGATGGAGATATATTTCTTACCTGTAGACCTATTATCATTTCCTAAATTAGTCGATGTGGGACTTAATCCTCGACATATGTTTGTAGGCATTTCGAAATGAATATCTCGTTTTTATTATATATATCACATTTTTAATCTATAATATGTCTAATTGGAAATAAGTATCTCGTTTTTAATCTATATATACATACATACATACATATATATATATACATACATACATATATATATACATACACATATATATAAATACATACATACATATATATATATACACACACATTACATTTTATGTTTTTTTTGCTTAGACAACGTAAACTTATGATGTATATCGATTGGGAAATGAATATCTCGTTCTTAATAGATATATATATGCCACATGTTATGTCTTTTTTTCAGACAACTTAAGCCTATGATATATGGGTAGGTATTTGACAATGAATATCTCGTTTTTAATCCTCTTTTTCTATATAATTTTGTGTGTATATACTTCCAGAAAATTATGCTTTTTAACCATTACCCATATACATTTGAAGGTGTTTAGGCAGCTGGATTTACCTCTGGAACGGCGTATTTCGCTTTGTGAAGAGAAAGGGGACATCGAGAGAGCAAAGCAACCTTGGAAGACTCTACCTTCTGGTCATAAGATTGGCACACCACAACCTTTATTCAAAGAATTGGTATGTATGCCTCATTGTGTTTAATAATTTGTTGTTGGTGGTGTCGATATAAATTGAGGATCCAGGATTATTATGGTGATTGTGGGTAACGAACCACCTTGGTGACAATTTTAACTCTCTTTCTCCAGAAAGACGAAGAAGTAGAACTATACAGAGACAAGTTTGCCGGAAGTCAGGCTCAACGCATTGTTAAAGCAGAAGCAGAAAAGCTTGCTGCTCACCTAAAAAAAACTAACGTTTCAGGTTGGTTGGTTAAAGTTTCGCCATTTTTCTTCTTTTACTTCCCGAATCCCGTTGTAGATTTTATGAATGACGTAAATTCTTCCCGGTCTTAAAAAGCTCCTTTTCAAGCTAATATTTTTATGTTTACTTCTACTTTGATTCCATTTTAGTCATTATTTGCTGCTAATTTTTTTAAAAAAATATTCAATAACGGGGATTCAAATATGTTCAAGTATATAGACCAGTTTAGCCAGATGTTTTTCTAGCAAATGTTTTTTTTTTTTTCTAAATGGTTTATTTGTTGTTGAAGGCAGTGAAAAAAAGCAACAGGCAAAATCCTCTTCAGGCCGCAAACCGAAAGCTGCTGCTGGTGGTGGTGCGGTGGAAGCAGGAATTACGATAACAAGACTGGATATACGAGTAGGATTAATTACTAAAGCTCAGAAGCATCCTGATGCTGATTCCCTATATGTGGAAGAAATCGATGTGGGAGAAGCGCAGCCTCGAACCGTTGTCAGTGGACTGGTCAACTACATACCTCTTCAAGAGATGCAGGTTTCCCTTGTTCTCTTCTTTACTTACATTGTTGTAATGAATAATATAATTAATGATCATTTTCTGCTGGTATAGAACCGGAAGGTCTGTGTTCTTTGCAACTTGAAGCCGGCTTCCATGAGGGGAATTAAATCGCAAGCCATGGTTCTGGCTGCTTCCAATAGCGACCACACCAAGGTTTGTCCTTCTTTGCAAGCTTTTCCAACTAAAAATACTCCTAAACTATCACCTATTGTTTTAAACATACTCATATCCTTTAAAAAAAAAAAAAAAAAAAAAAGGAATGCACATTCATAAATATTTTAGTAGAAATTCATTAAAATCCTAAATGAAAATTGGAAATTTGAAACTTGCTCACTAAAGTAGGAATTCATTAAAATCCTAAGTGAAAATTGGAAATTGGAAATTTGAAACTTATACTCGTATCATTTCAAAAAAAGTTGCAATATTCCTAGATCATTCATAAATACTTCAAAACTATTCATTAATGTAGAAATCAATTAAAATTTTGAATAAAAATTGGGGATTTGAAGTTATCATTTTTAGATGAAAAAAAAAAACATATTTCATCATTGAATATTTTCAATCGTGATTTTAACCAAACAGGAGTGTAAAAAGAACTTGTGATTATGATGGTGCACTTTTTTAGGTTGAATTAGTCGAGCCACCCAAAGACGCCAATGTCGGAGAAAGAATAAAATTCCCAGGATTCGAAGGCGAGCCCGAGGACGTACTAAACCCGAAGAAAAAGGTATGGGAGACGCTGCAAGTGGATTTGGCTATCAACACAGAGGGGGTGGCATGTTTTAAAGATATACCATTTAACACGTCGGCTGGCATTTGCAAAGTTCCTTCCATTACCAGTGGCTCTATCTGGTAG

mRNA sequence

ATGGCGGACGTCTTAGGCGACGCCGGTGGCGGCGGAACCAAGGCCACAAAGCTTCCGATTCCAGGAAAGAGGAATATACTCATTACCAGCGCTCTTCCATACGTCAATAACGTTCCTCACCTCGGCAACATCATTGGCTGTGTGTTGAGTGCTGATGTTTTTGCTCGGTACTGTCGTCTTAGAGGTTATAACGCTATTTATATATGCGGAACCGATGAGTATGGAACGGCGACGGAGACAAAAGCAATGGAAGAGAAATGTTCTCCTAAAGAGATTTGTGACAAGTACCACAAAATTCACAAAGAAGTTTACGATTGGTTTGGTATTAGTTTTGATGAATTTGGTCGCACATCATCATCACAGCAGACTGAAGTTTGTCAAGAAATATTTGGAAAACTATTGGAGAACAATTGGCTTTCTGAGAACACGATGCTACAGCTGTACTGTGATACTTGTGAGAGATTTTTAGCCGATCGGCTCGTTGAAGGCATCTGTCCTACTCCAGGTTGTGAGTATGACTCTGCACGTGGTGATCAATGTGAAAAGTGCGGGAAACTTCTTAATCCAACGGAGTTGAAAGATCCCAGGTGCAAGGTCTGTCAAACATCCCCACGTATCCGTGACACAAACCACATGTTTCTTGAACTTCCTTTGCTGAGAGATAAACTTGAAGAATATATCAACAAGATGTCTGTGGCTGGATCTTGGAGCCAAAATGCTATTCAAGCAACACAAGCTTGGCTTAAGGAAGGGTTGAAACCCCGGTGTATTACAAGAGATCTAAAGTGGGGGGTTCCCGTTCCACTTGAAAGGTTCAAGGACAAGGTCTTCTATGTTTGGTTTGATGCACCAATAGGATATGTCTCCATTACATCATGCTACACAAATGAATGGGAGAAATGGTGGAAGAATCCAGAGAATGTGGAGCTCTTTCAATTCATGGGCAAGGATAATGTTCCTTTCCACACTGTAGAAGTATGGAGATATTACTTATTAGCCAATAGGCCTGAGGTTTCAGATACATTATTTACATGGGCTGATTTACAAGCAAAACTCAATGGCGAGTTGTTGAACAATTTGGGCAACTTCATTCATCGAGTTTTGAGCTTCATTGCAAAACCTTTAGGGCAGGGTTATGGTTCCATAATTCCTGATGCACCTAACGCGGACTCTCATGGATTAACATTGGGTTTGGCTGAAAAAGTTGGTAAATACGTAGACCAATATGTGGAATCAATGGAGAAGGTTAAACTAAAACAAGGTCTAAAAGCTGCAATGGCTATTTCAAGCGAGGGAAATACATATTTGCAAACATCCCAGTTCTGGAATCTTTACAAGGCGGACAAACCTTCGTGCAATATTGTTATGAGAACTTCAACTGGACTTGTGCATCTTCTGGCATGTTTATTAGAACCTTTCATGCCTTCTTTCTCAGATGAGGTGTTTAGGCAGCTGGATTTACCTCTGGAACGGCGTATTTCGCTTTGTGAAGAGAAAGGGGACATCGAGAGAGCAAAGCAACCTTGGAAGACTCTACCTTCTGGTCATAAGATTGGCACACCACAACCTTTATTCAAAGAATTGAAAGACGAAGAAGTAGAACTATACAGAGACAAGTTTGCCGGAAGTCAGGCTCAACGCATTGTTAAAGCAGAAGCAGAAAAGCTTGCTGCTCACCTAAAAAAAACTAACGTTTCAGGCAGTGAAAAAAAGCAACAGGCAAAATCCTCTTCAGGCCGCAAACCGAAAGCTGCTGCTGGTGGTGGTGCGGTGGAAGCAGGAATTACGATAACAAGACTGGATATACGAGTAGGATTAATTACTAAAGCTCAGAAGCATCCTGATGCTGATTCCCTATATGTGGAAGAAATCGATGTGGGAGAAGCGCAGCCTCGAACCGTTGTCAGTGGACTGGTCAACTACATACCTCTTCAAGAGATGCAGAACCGGAAGGTCTGTGTTCTTTGCAACTTGAAGCCGGCTTCCATGAGGGGAATTAAATCGCAAGCCATGGTTCTGGCTGCTTCCAATAGCGACCACACCAAGGTTGAATTAGTCGAGCCACCCAAAGACGCCAATGTCGGAGAAAGAATAAAATTCCCAGGATTCGAAGGCGAGCCCGAGGACGTACTAAACCCGAAGAAAAAGGTATGGGAGACGCTGCAAGTGGATTTGGCTATCAACACAGAGGGGGTGGCATGTTTTAAAGATATACCATTTAACACGTCGGCTGGCATTTGCAAAGTTCCTTCCATTACCAGTGGCTCTATCTGGTAG

Coding sequence (CDS)

ATGGCGGACGTCTTAGGCGACGCCGGTGGCGGCGGAACCAAGGCCACAAAGCTTCCGATTCCAGGAAAGAGGAATATACTCATTACCAGCGCTCTTCCATACGTCAATAACGTTCCTCACCTCGGCAACATCATTGGCTGTGTGTTGAGTGCTGATGTTTTTGCTCGGTACTGTCGTCTTAGAGGTTATAACGCTATTTATATATGCGGAACCGATGAGTATGGAACGGCGACGGAGACAAAAGCAATGGAAGAGAAATGTTCTCCTAAAGAGATTTGTGACAAGTACCACAAAATTCACAAAGAAGTTTACGATTGGTTTGGTATTAGTTTTGATGAATTTGGTCGCACATCATCATCACAGCAGACTGAAGTTTGTCAAGAAATATTTGGAAAACTATTGGAGAACAATTGGCTTTCTGAGAACACGATGCTACAGCTGTACTGTGATACTTGTGAGAGATTTTTAGCCGATCGGCTCGTTGAAGGCATCTGTCCTACTCCAGGTTGTGAGTATGACTCTGCACGTGGTGATCAATGTGAAAAGTGCGGGAAACTTCTTAATCCAACGGAGTTGAAAGATCCCAGGTGCAAGGTCTGTCAAACATCCCCACGTATCCGTGACACAAACCACATGTTTCTTGAACTTCCTTTGCTGAGAGATAAACTTGAAGAATATATCAACAAGATGTCTGTGGCTGGATCTTGGAGCCAAAATGCTATTCAAGCAACACAAGCTTGGCTTAAGGAAGGGTTGAAACCCCGGTGTATTACAAGAGATCTAAAGTGGGGGGTTCCCGTTCCACTTGAAAGGTTCAAGGACAAGGTCTTCTATGTTTGGTTTGATGCACCAATAGGATATGTCTCCATTACATCATGCTACACAAATGAATGGGAGAAATGGTGGAAGAATCCAGAGAATGTGGAGCTCTTTCAATTCATGGGCAAGGATAATGTTCCTTTCCACACTGTAGAAGTATGGAGATATTACTTATTAGCCAATAGGCCTGAGGTTTCAGATACATTATTTACATGGGCTGATTTACAAGCAAAACTCAATGGCGAGTTGTTGAACAATTTGGGCAACTTCATTCATCGAGTTTTGAGCTTCATTGCAAAACCTTTAGGGCAGGGTTATGGTTCCATAATTCCTGATGCACCTAACGCGGACTCTCATGGATTAACATTGGGTTTGGCTGAAAAAGTTGGTAAATACGTAGACCAATATGTGGAATCAATGGAGAAGGTTAAACTAAAACAAGGTCTAAAAGCTGCAATGGCTATTTCAAGCGAGGGAAATACATATTTGCAAACATCCCAGTTCTGGAATCTTTACAAGGCGGACAAACCTTCGTGCAATATTGTTATGAGAACTTCAACTGGACTTGTGCATCTTCTGGCATGTTTATTAGAACCTTTCATGCCTTCTTTCTCAGATGAGGTGTTTAGGCAGCTGGATTTACCTCTGGAACGGCGTATTTCGCTTTGTGAAGAGAAAGGGGACATCGAGAGAGCAAAGCAACCTTGGAAGACTCTACCTTCTGGTCATAAGATTGGCACACCACAACCTTTATTCAAAGAATTGAAAGACGAAGAAGTAGAACTATACAGAGACAAGTTTGCCGGAAGTCAGGCTCAACGCATTGTTAAAGCAGAAGCAGAAAAGCTTGCTGCTCACCTAAAAAAAACTAACGTTTCAGGCAGTGAAAAAAAGCAACAGGCAAAATCCTCTTCAGGCCGCAAACCGAAAGCTGCTGCTGGTGGTGGTGCGGTGGAAGCAGGAATTACGATAACAAGACTGGATATACGAGTAGGATTAATTACTAAAGCTCAGAAGCATCCTGATGCTGATTCCCTATATGTGGAAGAAATCGATGTGGGAGAAGCGCAGCCTCGAACCGTTGTCAGTGGACTGGTCAACTACATACCTCTTCAAGAGATGCAGAACCGGAAGGTCTGTGTTCTTTGCAACTTGAAGCCGGCTTCCATGAGGGGAATTAAATCGCAAGCCATGGTTCTGGCTGCTTCCAATAGCGACCACACCAAGGTTGAATTAGTCGAGCCACCCAAAGACGCCAATGTCGGAGAAAGAATAAAATTCCCAGGATTCGAAGGCGAGCCCGAGGACGTACTAAACCCGAAGAAAAAGGTATGGGAGACGCTGCAAGTGGATTTGGCTATCAACACAGAGGGGGTGGCATGTTTTAAAGATATACCATTTAACACGTCGGCTGGCATTTGCAAAGTTCCTTCCATTACCAGTGGCTCTATCTGGTAG

Protein sequence

MADVLGDAGGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSSQQTEVCQEIFGKLLENNWLSENTMLQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCEKCGKLLNPTELKDPRCKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNAIQATQAWLKEGLKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEKWWKNPENVELFQFMGKDNVPFHTVEVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVLSFIAKPLGQGYGSIIPDAPNADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQGLKAAMAISSEGNTYLQTSQFWNLYKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFSDEVFRQLDLPLERRISLCEEKGDIERAKQPWKTLPSGHKIGTPQPLFKELKDEEVELYRDKFAGSQAQRIVKAEAEKLAAHLKKTNVSGSEKKQQAKSSSGRKPKAAAGGGAVEAGITITRLDIRVGLITKAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKPASMRGIKSQAMVLAASNSDHTKVELVEPPKDANVGERIKFPGFEGEPEDVLNPKKKVWETLQVDLAINTEGVACFKDIPFNTSAGICKVPSITSGSIW
Homology
BLAST of Csor.00g257440 vs. ExPASy Swiss-Prot
Match: Q9ZTS1 (Probable methionine--tRNA ligase OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0508700 PE=2 SV=2)

HSP 1 Score: 1149.8 bits (2973), Expect = 0.0e+00
Identity = 562/798 (70.43%), Postives = 631/798 (79.07%), Query Frame = 0

Query: 17  KLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYNAIYICGTDEYGT 76
           KLP+PG+RNIL+TSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYN IYICGTDEYGT
Sbjct: 9   KLPVPGRRNILVTSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYNVIYICGTDEYGT 68

Query: 77  ATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSSQQTEVCQEIFGKLLEN 136
           ATETKAMEEKCSPKEICDKYH +H EVY WF I FD+FGRTSS QQTEVCQ IF KL+EN
Sbjct: 69  ATETKAMEEKCSPKEICDKYHAVHSEVYKWFDIKFDKFGRTSSPQQTEVCQAIFQKLMEN 128

Query: 137 NWLSENTMLQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCEKCGKLLNPTELKDPR 196
           NWL+ENTM QLYCDTC+RFLADRLVEG CPT GC Y++ARGDQCE C KLLNPTEL DP+
Sbjct: 129 NWLTENTMQQLYCDTCQRFLADRLVEGKCPTEGCNYEAARGDQCENCSKLLNPTELIDPK 188

Query: 197 CKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNAIQATQAWLKEGLKPRC 256
           CKVC+ +PR+RDT+H+FLELPLL DKL  YIN+ SVAG WSQNAIQAT AWLKEGLKPRC
Sbjct: 189 CKVCKNTPRVRDTDHLFLELPLLSDKLVNYINETSVAGMWSQNAIQATNAWLKEGLKPRC 248

Query: 257 ITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEKWWKNPENVELFQFMGK 316
           ITRDLKWGVPVP E++KDKVFYVWFDAPIGYVSIT+ YT +WEKWWK+P+NVELFQFMGK
Sbjct: 249 ITRDLKWGVPVPHEKYKDKVFYVWFDAPIGYVSITASYTPDWEKWWKDPDNVELFQFMGK 308

Query: 317 DNVPFHTV---------------------------------------------------- 376
           DNVPFHTV                                                    
Sbjct: 309 DNVPFHTVMFPSTLLGTGEKWTMMKTISVTEYLNYEAGKFSKSHGIGVFGNDAKDTNIPP 368

Query: 377 EVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVLSFIAKPLGQGYGSIIP 436
           EVWRYYLL NRPEVSDTLFTWADLQAKLN ELLNNLGNFI+RVLSF+AKP G GY SI+P
Sbjct: 369 EVWRYYLLTNRPEVSDTLFTWADLQAKLNSELLNNLGNFINRVLSFVAKPAGAGYDSIVP 428

Query: 437 DAPNADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQGLKAAMAISSEGNTYLQTSQFWNL 496
           DAPNA+SH LT  L EK  K+V+QY+E+MEKVKLKQGLK+AM ISS+GN YLQ SQFW L
Sbjct: 429 DAPNAESHPLTKALVEKTNKWVEQYLEAMEKVKLKQGLKSAMGISSDGNAYLQESQFWKL 488

Query: 497 YKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFSDEVFRQLDLPLERRISLCEEKGDIER 556
           YK D  +C +VM+TS G+V+LLACLLEPFMPSFS+EV  QL++  E  +S C++KG+I +
Sbjct: 489 YKEDPAACAVVMKTSVGVVYLLACLLEPFMPSFSNEVLLQLNMTPEESLSFCDDKGEIAK 548

Query: 557 AKQPWKTLPSGHKIGTPQPLFKELKDEEVELYRDKFAGSQAQRIVK----AEAEKLAAHL 616
           AK+PW  + +GHKIG P PLFKELKDEEVE +R+KFAGSQA+R  K    AEA+K+A  L
Sbjct: 549 AKRPWDFVSAGHKIGKPSPLFKELKDEEVESFRNKFAGSQAERSSKAQADAEAKKVADKL 608

Query: 617 KKTNVS-GSEKKQQAKSSSGRKPKAAAGGGAVEAGITITRLDIRVGLITKAQKHPDADSL 676
           K T +S G +KK+Q K S G K K A      E  +T+ +LDIRVGLI KAQKHPDADSL
Sbjct: 609 KGTKLSDGGQKKEQKKQSGGSKSKNA------EVDVTVAKLDIRVGLIRKAQKHPDADSL 668

Query: 677 YVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKPASMRGIKSQAMVLAASNSD 736
           YVEEIDVGE  PRTVVSGLV +IPL+EMQNRKVCVLCNLKP +MRGIKS AMVLAASN D
Sbjct: 669 YVEEIDVGEEAPRTVVSGLVKFIPLEEMQNRKVCVLCNLKPVAMRGIKSHAMVLAASNED 728

Query: 737 HTKVELVEPPKDANVGERIKFPGFEGEPEDVLNPKKKVWETLQVDLAINTEGVACFKDIP 758
           HTKVELVEPP+ A VGER+ F G+ GEPE  LN K K WE L  DL  N E VAC+KD+P
Sbjct: 729 HTKVELVEPPESAAVGERVTFAGYSGEPEASLNAKSKTWEKLSADLHSNGELVACYKDVP 788

BLAST of Csor.00g257440 vs. ExPASy Swiss-Prot
Match: Q9SVN5 (Methionine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At4g13780 PE=2 SV=1)

HSP 1 Score: 1147.1 bits (2966), Expect = 0.0e+00
Identity = 564/798 (70.68%), Postives = 636/798 (79.70%), Query Frame = 0

Query: 12  GTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYNAIYICGT 71
           G  + KLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADV+ARYCRLRGYNAIYICGT
Sbjct: 5   GKSSPKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVYARYCRLRGYNAIYICGT 64

Query: 72  DEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSSQQTEVCQEIFG 131
           DEYGTATETKA+EE C+PKEICDKYH IHKEVYDWFGISFD+FGRTS+ +QTEVCQ IF 
Sbjct: 65  DEYGTATETKALEENCTPKEICDKYHAIHKEVYDWFGISFDKFGRTSTPEQTEVCQAIFN 124

Query: 132 KLLENNWLSENTMLQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCEKCGKLLNPTE 191
           KL +N WLSENTM QLYCDTC++FLADRLVEG CP  GC YDSARGDQCEKCGKLLNPTE
Sbjct: 125 KLWDNKWLSENTMQQLYCDTCKKFLADRLVEGSCPFEGCNYDSARGDQCEKCGKLLNPTE 184

Query: 192 LKDPRCKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNAIQATQAWLKEG 251
           LKDP+CKVCQ +PRIRDT+H+F+ELPLL+D+LE YI K SV GSWSQNAIQ T AWL++G
Sbjct: 185 LKDPKCKVCQNTPRIRDTDHLFIELPLLKDRLEAYIKKTSVTGSWSQNAIQTTNAWLRDG 244

Query: 252 LKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEKWWKNPENVELF 311
           L+ RCITRDLKWGVPVP E++KDKVFYVWFDAPIGYVSITSCYT+EWEKWWKNPENVEL+
Sbjct: 245 LRQRCITRDLKWGVPVPHEKYKDKVFYVWFDAPIGYVSITSCYTSEWEKWWKNPENVELY 304

Query: 312 QFMGKDNVPFHT------------------------------------------------ 371
           QFMGKDNVPFHT                                                
Sbjct: 305 QFMGKDNVPFHTVMFPSTQLGTEENWTLMKTISVTEYLNYEDGKFSKSKGVGVFGNDVKD 364

Query: 372 ----VEVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVLSFIAKPLGQGY 431
               VEVWRYYLL NRPEVSDT F+W DLQAKLNGELL+NLGNF++RVLSFIAKP   GY
Sbjct: 365 TNIPVEVWRYYLLTNRPEVSDTSFSWTDLQAKLNGELLSNLGNFVNRVLSFIAKPDNAGY 424

Query: 432 GSIIPDAPNADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQGLKAAMAISSEGNTYLQTS 491
           GS+IPDA +A+SH LT  LAEKV K+V +YVE+MEKVKLKQGLK AM ISSEGN YLQ S
Sbjct: 425 GSVIPDAHDAESHSLTKSLAEKVEKFVAEYVEAMEKVKLKQGLKTAMLISSEGNYYLQAS 484

Query: 492 QFWNLYKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFSDEVFRQLDLPLERRISLCEEK 551
           QFW LYK DKP C +V+RT+ GLVHLLA LLEPFMPSFS EVF+QL+LP   + SL +E+
Sbjct: 485 QFWKLYKEDKPLCAVVIRTAAGLVHLLAQLLEPFMPSFSCEVFKQLNLP--PQFSLSDER 544

Query: 552 GDIERAKQPWKTLPSGHKIGTPQPLFKELKDEEVELYRDKFAGSQAQRIVKAEAEKLAAH 611
           G++  A +PW  LP  H+IGTPQPLFKEL+++EV  YR+KFAGSQ+ R  + EA  LA  
Sbjct: 545 GEVLLASRPWDILPPSHRIGTPQPLFKELENDEVARYREKFAGSQSDRRARDEAANLADQ 604

Query: 612 LKKTNVSGSEKKQQAKSSSGRKPKAAAGGGAVEAGITITRLDIRVGLITKAQKHPDADSL 671
           L KT +S + KKQ+A S  G KPK      A +  IT+ RLDIRVG I KA+KHP AD+L
Sbjct: 605 LNKTKLSDA-KKQKASSKGGGKPKPQP---AADREITMARLDIRVGKIVKAEKHPKADAL 664

Query: 672 YVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKPASMRGIKSQAMVLAASNSD 731
           YVEEIDVG  + RTVVSGLV YIPL+EMQNR VCVLCNLKPA MR I SQAMVLAAS+SD
Sbjct: 665 YVEEIDVGGGEIRTVVSGLVKYIPLEEMQNRMVCVLCNLKPAKMRDIVSQAMVLAASSSD 724

Query: 732 HTKVELVEPPKDANVGERIKFPGFEGEPEDVLNPKKKVWETLQVDLAINTEGVACFKDIP 758
            +KVELVEPPK AN+GER+ FPGFEGEP+DVLNPKKKVWETL VDL      VAC+KD+P
Sbjct: 725 GSKVELVEPPKTANIGERVTFPGFEGEPDDVLNPKKKVWETLLVDLNTKENLVACYKDVP 784

BLAST of Csor.00g257440 vs. ExPASy Swiss-Prot
Match: Q20970 (Methionine--tRNA ligase, cytoplasmic OS=Caenorhabditis elegans OX=6239 GN=mars-1 PE=3 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 2.8e-188
Identity = 375/896 (41.85%), Postives = 493/896 (55.02%), Query Frame = 0

Query: 18  LPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYNAIYICGTDEYGTA 77
           LP PGKRNILIT+ALPYVNNVPHLGNIIGCVLSADVFARYC LRG+   Y+ GTDEYGTA
Sbjct: 29  LPQPGKRNILITAALPYVNNVPHLGNIIGCVLSADVFARYCNLRGHQTFYVGGTDEYGTA 88

Query: 78  TETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSSQQTEVCQEIFGKLLENN 137
           TETKA++E C+P+E+CDKYH IHK +Y+WFGI F  FGRT++  QTE+CQ++F KL +N 
Sbjct: 89  TETKALQEGCTPRELCDKYHAIHKGIYEWFGIDFSHFGRTTTDHQTEICQDMFLKLHKNG 148

Query: 138 WLSENTMLQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCEKCGKLLNPTELKDPRC 197
           + S  ++ QLYC+ CE+FLADR V G CP   C YD ARGDQC+ CGKL+N  +LKD +C
Sbjct: 149 YTSSQSVDQLYCNQCEKFLADRFVTGTCPM--CAYDDARGDQCDGCGKLINAVDLKDAKC 208

Query: 198 KVCQTSPRIRDTNHMFLELPLLRDKLEEYINK--MSVAGSWSQNAIQATQAWLKEGLKPR 257
            +C+ +P ++ + H+FL L  L+ K  E++++        WS NA+  T+AW+K GL PR
Sbjct: 209 HMCKATPEVKQSTHIFLSLDKLQQKTTEHLDRELAKEDNRWSSNAVGITKAWMKLGLDPR 268

Query: 258 CITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNE-WEKWWKNPENVELFQFM 317
           CITRDLKWG  VPL+ F+ KVFYVWFDAPIGY+SIT C   + W KWWKNPENVELF F+
Sbjct: 269 CITRDLKWGTAVPLDGFEKKVFYVWFDAPIGYLSITKCVLGDNWTKWWKNPENVELFNFV 328

Query: 318 GKDNVPFHTV-------------------------------------------------- 377
           GKDNV FH V                                                  
Sbjct: 329 GKDNVAFHAVMFPCSQLGANDNYTVVNNLCATEYLNYEDTKFSKSRGTGIFGDAAQGTEI 388

Query: 378 --EVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVLSFIAKPLGQGYGSI 437
             ++WR+YLL  RPE  DT F+W D   K+N ELLNNLGNFI+R LSF+A      +G +
Sbjct: 389 PADIWRFYLLYMRPESQDTAFSWDDFVLKVNSELLNNLGNFINRALSFVA----NSFGGV 448

Query: 438 IPD--APNADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQGLKAAMAISSEGNTYLQTSQ 497
           +P+    N D+  L+     ++     Q+ +  + V LK  +K  + +S  GN Y+Q   
Sbjct: 449 VPEMNLTNDDAEVLS-----EIHNECMQWDKQFDGVHLKDAVKTILNVSRLGNQYMQAQT 508

Query: 498 FWNLYKAD---KPSCNIVMRTSTGLVHLLACLLEPFMPSFSDEVFRQLD---LPLERRIS 557
            W L K D   K     ++  +  + + ++ LL P MP+ S  +  Q     LPL     
Sbjct: 509 PWVLMKKDEEGKKRAGTIIGVAANIAYHVSVLLYPIMPTISATIREQCGLPALPLFTPFP 568

Query: 558 LCEEKGDIERAKQPWKTLPSGHKIGTPQPLFKELKDEEVELYRDKFAGSQ-AQRIVKAEA 617
           +C               L +GHKIG P PLF++L   ++  ++ KF GSQ AQ      A
Sbjct: 569 IC--------------YLKAGHKIGQPSPLFQKLDPAQIAEFKAKFGGSQDAQSSAPKTA 628

Query: 618 EK--------------------------------------LAAHLKK------------- 677
           EK                                      +A +LKK             
Sbjct: 629 EKPKQQKKQAPTKDKKGDKKMASTAAFVELEQGAKVISQLIAQNLKKFDQAKALFTRNQL 688

Query: 678 ----------------------------------------------TNVSG----SEKKQ 737
                                                         T  SG    + KK+
Sbjct: 689 QRLDGENKQLTIDVKTLQHQLIELETAAGIKQVPKPVVSCTPTPTSTPASGIITEAPKKE 748

Query: 738 QAKSSSGRKPKAA------AGGGA---VEAGITITRLDIRVGLITKAQKHPDADSLYVEE 740
              + +  +PK A       GG A   V+  I + RLD+RVG I K +KHPDAD+LYVE+
Sbjct: 749 APSTPAPSEPKKAKEQKKGKGGAAAAPVDDTIDVGRLDMRVGRIIKCEKHPDADALYVEQ 808

BLAST of Csor.00g257440 vs. ExPASy Swiss-Prot
Match: Q54X95 (Probable methionine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=metS PE=3 SV=1)

HSP 1 Score: 632.5 bits (1630), Expect = 6.2e-180
Identity = 332/766 (43.34%), Postives = 457/766 (59.66%), Query Frame = 0

Query: 18  LPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYNAIYICGTDEYGTA 77
           LP  G+RNILITSALPYVNNVPHLGNIIGCVLSADV+ARYCRL+ YN IYICGTDEYGTA
Sbjct: 10  LPKDGERNILITSALPYVNNVPHLGNIIGCVLSADVYARYCRLKNYNCIYICGTDEYGTA 69

Query: 78  TETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSSQQTEVCQEIFGKLLENN 137
           TETKA+ E C+PKEICDKYH+IHKE+Y+WF ISFD+FGRTS++ QTE+ Q+IF K+ +N 
Sbjct: 70  TETKALSEGCTPKEICDKYHEIHKEIYEWFNISFDKFGRTSTNSQTEIAQDIFNKIKDNG 129

Query: 138 WLSENTMLQLYCD-TCERFLADRLVEGICPTPGCEYDSARGDQCEKCGKLLNPTELKDPR 197
           +     + QLYC+ TC+ FLADR VEG C  P C+++ ARGDQC+ C KLLNPTEL +PR
Sbjct: 130 YTLTQEIEQLYCEQTCKMFLADRFVEGTC--PHCKFEDARGDQCDGCSKLLNPTELINPR 189

Query: 198 CKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNAIQATQAWLKEGLKPRC 257
           CKVC   P I+ T H+F++LP L+ ++++++   S  G+WS+N+I  T  W+K  LKPRC
Sbjct: 190 CKVCSKPPVIKSTKHIFIDLPQLQQQVDQFVETNSKGGNWSENSIAITNTWVKGELKPRC 249

Query: 258 ITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEKWWKNPENVELFQFMGK 317
           ITRDLKWG PVP+E FKDKVFYVWFDAPIGY+SIT+ YTNEWEKWWKNPENV+L QFMGK
Sbjct: 250 ITRDLKWGTPVPMEEFKDKVFYVWFDAPIGYISITAEYTNEWEKWWKNPENVKLVQFMGK 309

Query: 318 DNVPFHTV---------------------------------------------------- 377
           DNVPFHTV                                                    
Sbjct: 310 DNVPFHTVIFPASLIGSKDNYTLLNNLSTTEFLNYETGKFSKSRNTGVFGDGAKATGIPS 369

Query: 378 EVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVLSFIAKPLGQGYGSIIP 437
           EVWR+YLL NRPE SD++F+W D   K N ELLNN GN ++RVL  +       +  ++P
Sbjct: 370 EVWRFYLLNNRPESSDSIFSWDDFNFK-NNELLNNFGNLVNRVLKML--NTNAAFNGVVP 429

Query: 438 DAPNADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQGLKAAMAISSEGNTYLQTSQFWNL 497
                + + +   L ++V +++ QY + +E++ LK+GLK AM+IS  GNTY+Q ++ W+L
Sbjct: 430 KI--GELNEVDKKLVQEVDEHLVQYFQKLEEISLKEGLKIAMSISKLGNTYMQDNKPWDL 489

Query: 498 YKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFSDEVFRQLDLPLERRISLCEEKGDIER 557
              D   C  V+     L+ LL+ LLEP++PS +D+V +QL++   +         DI  
Sbjct: 490 SGKDNERCGQVLAILINLIKLLSTLLEPYIPSLTDKVHQQLNVEPTK----YSTHFDI-- 549

Query: 558 AKQPWKTLPSGHKIGTP-QPLFKELKDEEVELYRDKFAGSQAQRIVKAEAEKLAAHLKKT 617
                  +P+GH+I     PL K+++ ++++ +R KF+G                     
Sbjct: 550 -----NAIPAGHEISKEILPLVKKIEADDLKKWRTKFSG--------------------- 609

Query: 618 NVSGSEKKQQAKSSSGRKPKAAAGGGAVEAGITITRLDIRVGLITKAQKHPDADSLYVEE 677
              G E                              +D+++  + +   HP A++LYV +
Sbjct: 610 --IGPE----------------------------FPIDMKIATVLEVNDHPSAENLYVIK 669

Query: 678 IDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKPASMRGIKSQAMVLAASNSDHTKV 726
           + +G  Q +T VSG+       ++  +K+ V+ NLKP+  +G+ S+AM+L A +   TK 
Sbjct: 670 LSLGGDQTKTAVSGIKANFEKSQLIGKKLAVVLNLKPSKFKGVLSEAMILVADDGQATKE 703

BLAST of Csor.00g257440 vs. ExPASy Swiss-Prot
Match: Q68FL6 (Methionine--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Mars1 PE=1 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 9.9e-162
Identity = 284/592 (47.97%), Postives = 376/592 (63.51%), Query Frame = 0

Query: 18  LPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYNAIYICGTDEYGTA 77
           LP+PG+RN+LITSALPYVNNVPHLGNIIGCVLSADVFARYCRLR +N +Y+CGTDEYGTA
Sbjct: 260 LPVPGERNVLITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRQWNTLYLCGTDEYGTA 319

Query: 78  TETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSSQQTEVCQEIFGKLLENN 137
           TETKAMEE  +P+EICDKYH IH ++Y WFGISFD FGRT++ QQT++ Q+IF +LL   
Sbjct: 320 TETKAMEEGLTPREICDKYHAIHADIYRWFGISFDTFGRTTTPQQTKITQDIFQRLLTRG 379

Query: 138 WLSENTMLQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCEKCGKLLNPTELKDPRC 197
           ++  +T+ QL C+ C RFLADR VEG+CP   C Y+ ARGDQC++CGKL+N  ELK P+C
Sbjct: 380 FVLRDTVEQLRCERCARFLADRFVEGVCPF--CGYEEARGDQCDRCGKLINAIELKKPQC 439

Query: 198 KVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNAIQATQAWLKEGLKPRCI 257
           K+C++ P +R + H+FL+LP L  +LE+++ K      W+ NA    ++WL++GLKPRCI
Sbjct: 440 KICRSCPVVRSSQHLFLDLPKLEKRLEDWLGKTVPGSDWTPNARFIIRSWLRDGLKPRCI 499

Query: 258 TRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEKWWKNPENVELFQFMGKD 317
           TRDLKWG PVPLE F+DKVFYVWFDA IGYVSIT+ YT++WEKWWKNPE V+L+QFM KD
Sbjct: 500 TRDLKWGTPVPLEGFEDKVFYVWFDATIGYVSITANYTDQWEKWWKNPEQVDLYQFMAKD 559

Query: 318 NVPFH----------------------------------------------------TVE 377
           NVPFH                                                      +
Sbjct: 560 NVPFHGLVFPCSVLGAEDNYTLVKHIIATEYLNYEDGKFSKSRGIGVFGDMAKDTGIPAD 619

Query: 378 VWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVLSFIAKPLGQGYGSIIPD 437
           +WR+YLL  RPE  D+ F+W DL  K N ELLNNLGNFI+R   F++K     +G  +P+
Sbjct: 620 IWRFYLLYIRPEGQDSAFSWTDLLIKNNSELLNNLGNFINRAGMFVSK----FFGGCVPE 679

Query: 438 -APNADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQGLKAAMAISSEGNTYLQTSQFWNL 497
            A   D       L   V   +  Y + +EKV+++  L++ + IS  GN Y+Q ++ W  
Sbjct: 680 MALTPDDR----RLVAHVSWELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKR 739

Query: 498 YKA---DKPSCNIVMRTSTGLVHLLACLLEPFMPSFSDEVFRQLDLPLERRISLCEEKGD 554
            K    D+     V   +  +  LL+ +L+P+MP+ S  +  QL LP      L      
Sbjct: 740 IKGGEMDRQRAGTVTGMAVNMAALLSVMLQPYMPTVSSTIQTQLQLPPAACRIL------ 799

BLAST of Csor.00g257440 vs. NCBI nr
Match: KAG6574842.1 (Methionine--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1550 bits (4014), Expect = 0.0
Identity = 758/758 (100.00%), Postives = 758/758 (100.00%), Query Frame = 0

Query: 1   MADVLGDAGGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL 60
           MADVLGDAGGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL
Sbjct: 1   MADVLGDAGGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL 60

Query: 61  RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSS 120
           RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSS
Sbjct: 61  RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSS 120

Query: 121 QQTEVCQEIFGKLLENNWLSENTMLQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC 180
           QQTEVCQEIFGKLLENNWLSENTMLQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC
Sbjct: 121 QQTEVCQEIFGKLLENNWLSENTMLQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC 180

Query: 181 EKCGKLLNPTELKDPRCKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNA 240
           EKCGKLLNPTELKDPRCKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNA
Sbjct: 181 EKCGKLLNPTELKDPRCKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNA 240

Query: 241 IQATQAWLKEGLKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEK 300
           IQATQAWLKEGLKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEK
Sbjct: 241 IQATQAWLKEGLKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEK 300

Query: 301 WWKNPENVELFQFMGKDNVPFHTVEVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNL 360
           WWKNPENVELFQFMGKDNVPFHTVEVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNL
Sbjct: 301 WWKNPENVELFQFMGKDNVPFHTVEVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNL 360

Query: 361 GNFIHRVLSFIAKPLGQGYGSIIPDAPNADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQ 420
           GNFIHRVLSFIAKPLGQGYGSIIPDAPNADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQ
Sbjct: 361 GNFIHRVLSFIAKPLGQGYGSIIPDAPNADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQ 420

Query: 421 GLKAAMAISSEGNTYLQTSQFWNLYKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFSDE 480
           GLKAAMAISSEGNTYLQTSQFWNLYKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFSDE
Sbjct: 421 GLKAAMAISSEGNTYLQTSQFWNLYKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFSDE 480

Query: 481 VFRQLDLPLERRISLCEEKGDIERAKQPWKTLPSGHKIGTPQPLFKELKDEEVELYRDKF 540
           VFRQLDLPLERRISLCEEKGDIERAKQPWKTLPSGHKIGTPQPLFKELKDEEVELYRDKF
Sbjct: 481 VFRQLDLPLERRISLCEEKGDIERAKQPWKTLPSGHKIGTPQPLFKELKDEEVELYRDKF 540

Query: 541 AGSQAQRIVKAEAEKLAAHLKKTNVSGSEKKQQAKSSSGRKPKAAAGGGAVEAGITITRL 600
           AGSQAQRIVKAEAEKLAAHLKKTNVSGSEKKQQAKSSSGRKPKAAAGGGAVEAGITITRL
Sbjct: 541 AGSQAQRIVKAEAEKLAAHLKKTNVSGSEKKQQAKSSSGRKPKAAAGGGAVEAGITITRL 600

Query: 601 DIRVGLITKAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKP 660
           DIRVGLITKAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKP
Sbjct: 601 DIRVGLITKAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKP 660

Query: 661 ASMRGIKSQAMVLAASNSDHTKVELVEPPKDANVGERIKFPGFEGEPEDVLNPKKKVWET 720
           ASMRGIKSQAMVLAASNSDHTKVELVEPPKDANVGERIKFPGFEGEPEDVLNPKKKVWET
Sbjct: 661 ASMRGIKSQAMVLAASNSDHTKVELVEPPKDANVGERIKFPGFEGEPEDVLNPKKKVWET 720

Query: 721 LQVDLAINTEGVACFKDIPFNTSAGICKVPSITSGSIW 758
           LQVDLAINTEGVACFKDIPFNTSAGICKVPSITSGSIW
Sbjct: 721 LQVDLAINTEGVACFKDIPFNTSAGICKVPSITSGSIW 758

BLAST of Csor.00g257440 vs. NCBI nr
Match: XP_022958743.1 (probable methionine--tRNA ligase [Cucurbita moschata])

HSP 1 Score: 1518 bits (3929), Expect = 0.0
Identity = 752/810 (92.84%), Postives = 757/810 (93.46%), Query Frame = 0

Query: 1   MADVLGDAGGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL 60
           MADVLGDAGGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL
Sbjct: 1   MADVLGDAGGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL 60

Query: 61  RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSS 120
           RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSS
Sbjct: 61  RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSS 120

Query: 121 QQTEVCQEIFGKLLENNWLSENTMLQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC 180
           QQTEVCQEIFGKLLENNWLSENTMLQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC
Sbjct: 121 QQTEVCQEIFGKLLENNWLSENTMLQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC 180

Query: 181 EKCGKLLNPTELKDPRCKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNA 240
           EKCGKLLNPTELKDPRCKVCQT+PRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNA
Sbjct: 181 EKCGKLLNPTELKDPRCKVCQTTPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNA 240

Query: 241 IQATQAWLKEGLKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEK 300
           IQATQAWLKEGLKPRCITRDLKWGVPVPLERF+DKVFYVWFDAPIGYVSITSCYTNEWEK
Sbjct: 241 IQATQAWLKEGLKPRCITRDLKWGVPVPLERFRDKVFYVWFDAPIGYVSITSCYTNEWEK 300

Query: 301 WWKNPENVELFQFMGKDNVPFHTV------------------------------------ 360
           WWKNPENVELFQFMGKDNVPFHTV                                    
Sbjct: 301 WWKNPENVELFQFMGKDNVPFHTVMFPSTLLGTGEDWTLMKTISVTEYLNYEAGKFSKSK 360

Query: 361 ----------------EVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVL 420
                           EVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVL
Sbjct: 361 GIGVFGNDAKDTNISAEVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVL 420

Query: 421 SFIAKPLGQGYGSIIPDAPNADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQGLKAAMAI 480
           SF+AKPLGQGYGSIIPDAPNADSHGLTL LAEKVGKYVDQYVESMEKVKLKQGLKAAMAI
Sbjct: 421 SFVAKPLGQGYGSIIPDAPNADSHGLTLVLAEKVGKYVDQYVESMEKVKLKQGLKAAMAI 480

Query: 481 SSEGNTYLQTSQFWNLYKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFSDEVFRQLDLP 540
           S+EGNTYLQTSQFWNLYKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFSDEVFRQLDLP
Sbjct: 481 SNEGNTYLQTSQFWNLYKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFSDEVFRQLDLP 540

Query: 541 LERRISLCEEKGDIERAKQPWKTLPSGHKIGTPQPLFKELKDEEVELYRDKFAGSQAQRI 600
           LERRISLCEEKGDIERAKQPWKTLPSGHKIGTPQPLFKELKDEEVELYRDKFAGSQAQRI
Sbjct: 541 LERRISLCEEKGDIERAKQPWKTLPSGHKIGTPQPLFKELKDEEVELYRDKFAGSQAQRI 600

Query: 601 VKAEAEKLAAHLKKTNVSGSEKKQQAKSSSGRKPKAAAGGGAVEAGITITRLDIRVGLIT 660
           VKAEAEKLAAHLKKTNVSGSEKKQQAKSS+GRKPKAAAGGGAVEAGITITRLDIRVGLIT
Sbjct: 601 VKAEAEKLAAHLKKTNVSGSEKKQQAKSSTGRKPKAAAGGGAVEAGITITRLDIRVGLIT 660

Query: 661 KAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKPASMRGIKS 720
           KAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKPASMRGIKS
Sbjct: 661 KAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKPASMRGIKS 720

Query: 721 QAMVLAASNSDHTKVELVEPPKDANVGERIKFPGFEGEPEDVLNPKKKVWETLQVDLAIN 758
           QAMVLAASNSDHTKVELVEPPKDANVGERIKFPGFEGEPEDVLNPKKKVWETLQVDLAIN
Sbjct: 721 QAMVLAASNSDHTKVELVEPPKDANVGERIKFPGFEGEPEDVLNPKKKVWETLQVDLAIN 780

BLAST of Csor.00g257440 vs. NCBI nr
Match: KAG7013416.1 (putative methionine--tRNA ligase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1510 bits (3910), Expect = 0.0
Identity = 757/835 (90.66%), Postives = 757/835 (90.66%), Query Frame = 0

Query: 1   MADVLGDAGGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL 60
           MADVLGDAGGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL
Sbjct: 1   MADVLGDAGGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL 60

Query: 61  RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSS 120
           RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSS
Sbjct: 61  RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSS 120

Query: 121 QQTEVCQEIFGKLLENNWLSENTMLQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC 180
           QQTEVCQEIFGKLLENNWLSENTMLQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC
Sbjct: 121 QQTEVCQEIFGKLLENNWLSENTMLQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC 180

Query: 181 EKCGKLLNPTELKDPRCKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNA 240
           EKCGKLLNPTELKDPRCKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNA
Sbjct: 181 EKCGKLLNPTELKDPRCKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNA 240

Query: 241 IQATQAWLKEGLKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEK 300
           IQATQAWLKEGLKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEK
Sbjct: 241 IQATQAWLKEGLKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEK 300

Query: 301 WWKNPENVELFQFMGKDNVPFHTV------------------------------------ 360
           WWKNPENVELFQFMGKDNVPFHTV                                    
Sbjct: 301 WWKNPENVELFQFMGKDNVPFHTVMFPSTLLGTGEDWTLMKTISVTEYLNYEAGKFSKSK 360

Query: 361 ----------------EVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVL 420
                           EVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVL
Sbjct: 361 GIGVFGNDAKDTNISAEVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVL 420

Query: 421 SFIAKPLGQGYGSIIPDAPNADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQGLKAAMAI 480
           SFIAKPLGQGYGSIIPDAPNADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQGLKAAMAI
Sbjct: 421 SFIAKPLGQGYGSIIPDAPNADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQGLKAAMAI 480

Query: 481 SSEGNTYLQTSQFWNLYKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFSDEVFRQLDLP 540
           SSEGNTYLQTSQFWNLYKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFSDEVFRQLDLP
Sbjct: 481 SSEGNTYLQTSQFWNLYKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFSDEVFRQLDLP 540

Query: 541 LERRISLCEEKGDIERAKQPWKTLPSGHKIGTPQPLFKELKDEEVELYRDKFAGSQAQRI 600
           LERRISLCEEKGDIERAKQPWKTLPSGHKIGTPQPLFKEL DEEVELYRDKFAGSQAQRI
Sbjct: 541 LERRISLCEEKGDIERAKQPWKTLPSGHKIGTPQPLFKELNDEEVELYRDKFAGSQAQRI 600

Query: 601 VKAEAEKLAAHLKKTNVSGSEKKQQAKSSSGRKPKAAAGGGAVEAGITITRLDIRVGLIT 660
           VKAEAEKLAAHLKKTNVSGSEKKQQAKSSSGRKPKAAAGGGAVEAGITITRLDIRVGLIT
Sbjct: 601 VKAEAEKLAAHLKKTNVSGSEKKQQAKSSSGRKPKAAAGGGAVEAGITITRLDIRVGLIT 660

Query: 661 KAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQ-------------------- 720
           KAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQ                    
Sbjct: 661 KAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQVSLVLFFTYIVVMNNIINDH 720

Query: 721 -----NRKVCVLCNLKPASMRGIKSQAMVLAASNSDHTKVELVEPPKDANVGERIKFPGF 758
                NRKVCVLCNLKPASMRGIKSQAMVLAASNSDHTKVELVEPPKDANVGERIKFPGF
Sbjct: 721 FLLVKNRKVCVLCNLKPASMRGIKSQAMVLAASNSDHTKVELVEPPKDANVGERIKFPGF 780

BLAST of Csor.00g257440 vs. NCBI nr
Match: XP_023006265.1 (probable methionine--tRNA ligase [Cucurbita maxima])

HSP 1 Score: 1498 bits (3879), Expect = 0.0
Identity = 746/814 (91.65%), Postives = 752/814 (92.38%), Query Frame = 0

Query: 1   MADVLGDAGGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL 60
           MADVLGDAGGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL
Sbjct: 1   MADVLGDAGGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL 60

Query: 61  RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSS 120
           RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSS
Sbjct: 61  RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSS 120

Query: 121 QQTEVCQEIFGKLLENNWLSENTMLQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC 180
           QQTEVCQEIFGKLLENNWLSENTM QLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC
Sbjct: 121 QQTEVCQEIFGKLLENNWLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC 180

Query: 181 EKCGKLLNPTELKDPRCKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNA 240
           EKCGKLLNPTELKDPRCKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNA
Sbjct: 181 EKCGKLLNPTELKDPRCKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNA 240

Query: 241 IQATQAWLKEGLKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEK 300
           IQATQAWLKEGLKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEK
Sbjct: 241 IQATQAWLKEGLKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEK 300

Query: 301 WWKNPENVELFQFMGKDNVPFHTV------------------------------------ 360
           WWKNPENVELFQFMGKDNVPFHTV                                    
Sbjct: 301 WWKNPENVELFQFMGKDNVPFHTVMFPSTLLGTGEDWTLMKTISVTEYLNYEAGKFSKSK 360

Query: 361 ----------------EVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVL 420
                           EVWRYYLL NRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVL
Sbjct: 361 GIGVFGNDAKDTNISAEVWRYYLLGNRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVL 420

Query: 421 SFIAKPLGQGYGSIIPDAPNADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQGLKAAMAI 480
           SFIAKPLGQGYGSIIPDAP+ADSHGLTLGLAEKV KYVDQYVESMEKVKLKQGLKAAMAI
Sbjct: 421 SFIAKPLGQGYGSIIPDAPDADSHGLTLGLAEKVDKYVDQYVESMEKVKLKQGLKAAMAI 480

Query: 481 SSEGNTYLQTSQFWNLYKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFSDEVFRQLDLP 540
           SSEGNTYLQ+SQFWNLYKADKPSCNIVMRTS+GLVHLLACLLEPFMPSFSDEVFRQLDLP
Sbjct: 481 SSEGNTYLQSSQFWNLYKADKPSCNIVMRTSSGLVHLLACLLEPFMPSFSDEVFRQLDLP 540

Query: 541 LERRISLCEEKGDIERAKQPWKTLPSGHKIGTPQPLFKELKDEEVELYRDKFAGSQAQRI 600
           LERRISLCEEKGDIERAKQPWKTLPSGHKIGTP PLFKELKDEEVELYRDKFAGSQAQRI
Sbjct: 541 LERRISLCEEKGDIERAKQPWKTLPSGHKIGTPHPLFKELKDEEVELYRDKFAGSQAQRI 600

Query: 601 VKAEAE----KLAAHLKKTNVSGSEKKQQAKSSSGRKPKAAAGGGAVEAGITITRLDIRV 660
           VKAEAE    KLAAHLKKTNVSGSEKKQQAKSS+GRKPKAA GGGAVEAGITITRLDIRV
Sbjct: 601 VKAEAEAEAEKLAAHLKKTNVSGSEKKQQAKSSTGRKPKAAGGGGAVEAGITITRLDIRV 660

Query: 661 GLITKAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKPASMR 720
           GLITKAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKPASMR
Sbjct: 661 GLITKAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKPASMR 720

Query: 721 GIKSQAMVLAASNSDHTKVELVEPPKDANVGERIKFPGFEGEPEDVLNPKKKVWETLQVD 758
           GIKSQAMVLAASNSDHTKVELVEPPKDANVGERIKFPGFEGEP+DVLNPKKKVWETLQVD
Sbjct: 721 GIKSQAMVLAASNSDHTKVELVEPPKDANVGERIKFPGFEGEPDDVLNPKKKVWETLQVD 780

BLAST of Csor.00g257440 vs. NCBI nr
Match: XP_023548158.1 (methionine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1493 bits (3865), Expect = 0.0
Identity = 742/811 (91.49%), Postives = 749/811 (92.36%), Query Frame = 0

Query: 1   MADVLGDAGGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL 60
           MADVLGDAGGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL
Sbjct: 1   MADVLGDAGGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL 60

Query: 61  RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSS 120
           RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSS
Sbjct: 61  RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSS 120

Query: 121 QQTEVCQEIFGKLLENNWLSENTMLQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC 180
           QQTEVCQEIFGKLLENNWLSENTM QLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC
Sbjct: 121 QQTEVCQEIFGKLLENNWLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC 180

Query: 181 EKCGKLLNPTELKDPRCKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNA 240
           EKCGKLLNPTELKDPRCKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNA
Sbjct: 181 EKCGKLLNPTELKDPRCKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNA 240

Query: 241 IQATQAWLKEGLKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEK 300
           IQATQAWLKEGLKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEK
Sbjct: 241 IQATQAWLKEGLKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEK 300

Query: 301 WWKNPENVELFQFMGKDNVPFHTV------------------------------------ 360
           WWKNPENVELFQFMGKDNVPFHTV                                    
Sbjct: 301 WWKNPENVELFQFMGKDNVPFHTVMFPSTLLGTGEDWTLMKTISVTEYLNYEAGKFSKSK 360

Query: 361 ----------------EVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVL 420
                           EVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVL
Sbjct: 361 GIGVFGNDAKDTNISAEVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVL 420

Query: 421 SFIAKPLGQGYGSIIPDAPNADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQGLKAAMAI 480
           SFIAKPLGQGYGSIIPDAP+ADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQGLKAAMAI
Sbjct: 421 SFIAKPLGQGYGSIIPDAPDADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQGLKAAMAI 480

Query: 481 SSEGNTYLQTSQFWNLYKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFSDEVFRQLDLP 540
           SSEGNTYLQTSQFWNLYKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFS EVFRQLDLP
Sbjct: 481 SSEGNTYLQTSQFWNLYKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFSHEVFRQLDLP 540

Query: 541 LERRISLCEEKGDIERAKQPWKTLPSGHKIGTPQPLFKELKDEEVELYRDKFAGSQAQRI 600
           LERRISLCEEKGDIERAKQPWKTLPSGHKIGTP PLFKELKDEEVE YRDKF GSQAQRI
Sbjct: 541 LERRISLCEEKGDIERAKQPWKTLPSGHKIGTPHPLFKELKDEEVEQYRDKFTGSQAQRI 600

Query: 601 VKAEAEKLAA-HLKKTNVSGSEKKQQAKSSSGRKPKAAAGGGAVEAGITITRLDIRVGLI 660
           VKAEAEKLAA HL+KTNVSG EKKQQAKSS+GRKPKAA GGGAVEAGITITRLDIRVGLI
Sbjct: 601 VKAEAEKLAAAHLEKTNVSGGEKKQQAKSSTGRKPKAAGGGGAVEAGITITRLDIRVGLI 660

Query: 661 TKAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKPASMRGIK 720
           TKAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKPASMRGIK
Sbjct: 661 TKAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKPASMRGIK 720

Query: 721 SQAMVLAASNSDHTKVELVEPPKDANVGERIKFPGFEGEPEDVLNPKKKVWETLQVDLAI 758
           SQAMVLAASNSDHTKVELVEPPKDANVGERIKFPGFEG+P+DVLNPKKKVWETLQVDLAI
Sbjct: 721 SQAMVLAASNSDHTKVELVEPPKDANVGERIKFPGFEGKPDDVLNPKKKVWETLQVDLAI 780

BLAST of Csor.00g257440 vs. ExPASy TrEMBL
Match: A0A6J1H3Y0 (Methionyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111459931 PE=3 SV=1)

HSP 1 Score: 1518 bits (3929), Expect = 0.0
Identity = 752/810 (92.84%), Postives = 757/810 (93.46%), Query Frame = 0

Query: 1   MADVLGDAGGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL 60
           MADVLGDAGGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL
Sbjct: 1   MADVLGDAGGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL 60

Query: 61  RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSS 120
           RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSS
Sbjct: 61  RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSS 120

Query: 121 QQTEVCQEIFGKLLENNWLSENTMLQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC 180
           QQTEVCQEIFGKLLENNWLSENTMLQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC
Sbjct: 121 QQTEVCQEIFGKLLENNWLSENTMLQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC 180

Query: 181 EKCGKLLNPTELKDPRCKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNA 240
           EKCGKLLNPTELKDPRCKVCQT+PRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNA
Sbjct: 181 EKCGKLLNPTELKDPRCKVCQTTPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNA 240

Query: 241 IQATQAWLKEGLKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEK 300
           IQATQAWLKEGLKPRCITRDLKWGVPVPLERF+DKVFYVWFDAPIGYVSITSCYTNEWEK
Sbjct: 241 IQATQAWLKEGLKPRCITRDLKWGVPVPLERFRDKVFYVWFDAPIGYVSITSCYTNEWEK 300

Query: 301 WWKNPENVELFQFMGKDNVPFHTV------------------------------------ 360
           WWKNPENVELFQFMGKDNVPFHTV                                    
Sbjct: 301 WWKNPENVELFQFMGKDNVPFHTVMFPSTLLGTGEDWTLMKTISVTEYLNYEAGKFSKSK 360

Query: 361 ----------------EVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVL 420
                           EVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVL
Sbjct: 361 GIGVFGNDAKDTNISAEVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVL 420

Query: 421 SFIAKPLGQGYGSIIPDAPNADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQGLKAAMAI 480
           SF+AKPLGQGYGSIIPDAPNADSHGLTL LAEKVGKYVDQYVESMEKVKLKQGLKAAMAI
Sbjct: 421 SFVAKPLGQGYGSIIPDAPNADSHGLTLVLAEKVGKYVDQYVESMEKVKLKQGLKAAMAI 480

Query: 481 SSEGNTYLQTSQFWNLYKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFSDEVFRQLDLP 540
           S+EGNTYLQTSQFWNLYKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFSDEVFRQLDLP
Sbjct: 481 SNEGNTYLQTSQFWNLYKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFSDEVFRQLDLP 540

Query: 541 LERRISLCEEKGDIERAKQPWKTLPSGHKIGTPQPLFKELKDEEVELYRDKFAGSQAQRI 600
           LERRISLCEEKGDIERAKQPWKTLPSGHKIGTPQPLFKELKDEEVELYRDKFAGSQAQRI
Sbjct: 541 LERRISLCEEKGDIERAKQPWKTLPSGHKIGTPQPLFKELKDEEVELYRDKFAGSQAQRI 600

Query: 601 VKAEAEKLAAHLKKTNVSGSEKKQQAKSSSGRKPKAAAGGGAVEAGITITRLDIRVGLIT 660
           VKAEAEKLAAHLKKTNVSGSEKKQQAKSS+GRKPKAAAGGGAVEAGITITRLDIRVGLIT
Sbjct: 601 VKAEAEKLAAHLKKTNVSGSEKKQQAKSSTGRKPKAAAGGGAVEAGITITRLDIRVGLIT 660

Query: 661 KAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKPASMRGIKS 720
           KAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKPASMRGIKS
Sbjct: 661 KAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKPASMRGIKS 720

Query: 721 QAMVLAASNSDHTKVELVEPPKDANVGERIKFPGFEGEPEDVLNPKKKVWETLQVDLAIN 758
           QAMVLAASNSDHTKVELVEPPKDANVGERIKFPGFEGEPEDVLNPKKKVWETLQVDLAIN
Sbjct: 721 QAMVLAASNSDHTKVELVEPPKDANVGERIKFPGFEGEPEDVLNPKKKVWETLQVDLAIN 780

BLAST of Csor.00g257440 vs. ExPASy TrEMBL
Match: A0A6J1KZN8 (Methionyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111499054 PE=3 SV=1)

HSP 1 Score: 1498 bits (3879), Expect = 0.0
Identity = 746/814 (91.65%), Postives = 752/814 (92.38%), Query Frame = 0

Query: 1   MADVLGDAGGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL 60
           MADVLGDAGGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL
Sbjct: 1   MADVLGDAGGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL 60

Query: 61  RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSS 120
           RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSS
Sbjct: 61  RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSS 120

Query: 121 QQTEVCQEIFGKLLENNWLSENTMLQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC 180
           QQTEVCQEIFGKLLENNWLSENTM QLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC
Sbjct: 121 QQTEVCQEIFGKLLENNWLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC 180

Query: 181 EKCGKLLNPTELKDPRCKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNA 240
           EKCGKLLNPTELKDPRCKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNA
Sbjct: 181 EKCGKLLNPTELKDPRCKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNA 240

Query: 241 IQATQAWLKEGLKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEK 300
           IQATQAWLKEGLKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEK
Sbjct: 241 IQATQAWLKEGLKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEK 300

Query: 301 WWKNPENVELFQFMGKDNVPFHTV------------------------------------ 360
           WWKNPENVELFQFMGKDNVPFHTV                                    
Sbjct: 301 WWKNPENVELFQFMGKDNVPFHTVMFPSTLLGTGEDWTLMKTISVTEYLNYEAGKFSKSK 360

Query: 361 ----------------EVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVL 420
                           EVWRYYLL NRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVL
Sbjct: 361 GIGVFGNDAKDTNISAEVWRYYLLGNRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVL 420

Query: 421 SFIAKPLGQGYGSIIPDAPNADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQGLKAAMAI 480
           SFIAKPLGQGYGSIIPDAP+ADSHGLTLGLAEKV KYVDQYVESMEKVKLKQGLKAAMAI
Sbjct: 421 SFIAKPLGQGYGSIIPDAPDADSHGLTLGLAEKVDKYVDQYVESMEKVKLKQGLKAAMAI 480

Query: 481 SSEGNTYLQTSQFWNLYKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFSDEVFRQLDLP 540
           SSEGNTYLQ+SQFWNLYKADKPSCNIVMRTS+GLVHLLACLLEPFMPSFSDEVFRQLDLP
Sbjct: 481 SSEGNTYLQSSQFWNLYKADKPSCNIVMRTSSGLVHLLACLLEPFMPSFSDEVFRQLDLP 540

Query: 541 LERRISLCEEKGDIERAKQPWKTLPSGHKIGTPQPLFKELKDEEVELYRDKFAGSQAQRI 600
           LERRISLCEEKGDIERAKQPWKTLPSGHKIGTP PLFKELKDEEVELYRDKFAGSQAQRI
Sbjct: 541 LERRISLCEEKGDIERAKQPWKTLPSGHKIGTPHPLFKELKDEEVELYRDKFAGSQAQRI 600

Query: 601 VKAEAE----KLAAHLKKTNVSGSEKKQQAKSSSGRKPKAAAGGGAVEAGITITRLDIRV 660
           VKAEAE    KLAAHLKKTNVSGSEKKQQAKSS+GRKPKAA GGGAVEAGITITRLDIRV
Sbjct: 601 VKAEAEAEAEKLAAHLKKTNVSGSEKKQQAKSSTGRKPKAAGGGGAVEAGITITRLDIRV 660

Query: 661 GLITKAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKPASMR 720
           GLITKAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKPASMR
Sbjct: 661 GLITKAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKPASMR 720

Query: 721 GIKSQAMVLAASNSDHTKVELVEPPKDANVGERIKFPGFEGEPEDVLNPKKKVWETLQVD 758
           GIKSQAMVLAASNSDHTKVELVEPPKDANVGERIKFPGFEGEP+DVLNPKKKVWETLQVD
Sbjct: 721 GIKSQAMVLAASNSDHTKVELVEPPKDANVGERIKFPGFEGEPDDVLNPKKKVWETLQVD 780

BLAST of Csor.00g257440 vs. ExPASy TrEMBL
Match: A0A6J1KIY2 (Methionyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111494895 PE=3 SV=1)

HSP 1 Score: 1380 bits (3573), Expect = 0.0
Identity = 692/811 (85.33%), Postives = 715/811 (88.16%), Query Frame = 0

Query: 1   MADVLGDAGGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL 60
           M DV GDA  GGTKAT LPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL
Sbjct: 1   MGDVSGDAAAGGTKATILPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL 60

Query: 61  RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSS 120
           RGYN IYICGTDEYGTATETKAMEEKCSPKEICDKYH IHKEVY+WFGISFDEFGRTSS 
Sbjct: 61  RGYNTIYICGTDEYGTATETKAMEEKCSPKEICDKYHAIHKEVYNWFGISFDEFGRTSSP 120

Query: 121 QQTEVCQEIFGKLLENNWLSENTMLQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC 180
           QQTEVCQ IFGKLLENNWLSENTM QLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC
Sbjct: 121 QQTEVCQAIFGKLLENNWLSENTMQQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC 180

Query: 181 EKCGKLLNPTELKDPRCKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNA 240
           EKCGKLLNPTELKDPRCKVCQT+PRIRDTNHMFLELPLLR+KLEEYINK SVAGSWSQNA
Sbjct: 181 EKCGKLLNPTELKDPRCKVCQTTPRIRDTNHMFLELPLLRNKLEEYINKTSVAGSWSQNA 240

Query: 241 IQATQAWLKEGLKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEK 300
           IQAT AWLKEGLKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEK
Sbjct: 241 IQATNAWLKEGLKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEK 300

Query: 301 WWKNPENVELFQFMGKDNVPFHTV------------------------------------ 360
           WWKNPENVELFQFMGKDNVPFHTV                                    
Sbjct: 301 WWKNPENVELFQFMGKDNVPFHTVMFPSTLLGTGEDWTLMKTISVTEYLNYEAGKFSKSK 360

Query: 361 ----------------EVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVL 420
                           EVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVL
Sbjct: 361 GVGVFGNDAKDTNIPVEVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVL 420

Query: 421 SFIAKPLGQGYGSIIPDAPNADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQGLKAAMAI 480
           SFIAKPLGQGYGSIIPD  +ADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQGLK+AMAI
Sbjct: 421 SFIAKPLGQGYGSIIPDVSDADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQGLKSAMAI 480

Query: 481 SSEGNTYLQTSQFWNLYKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFSDEVFRQLDLP 540
           S+EGN YLQT+QFWNLYK DKPSCN+VMRTS GLV+LLACLLEPFMPSFS EVF+QL++P
Sbjct: 481 STEGNIYLQTAQFWNLYKEDKPSCNLVMRTSAGLVYLLACLLEPFMPSFSQEVFKQLNIP 540

Query: 541 LERRISLCEEKGDIERAKQPWKTLPSGHKIGTPQPLFKELKDEEVELYRDKFAGSQAQRI 600
           +ER +SLCEEKGDIERAKQPWK LPSGHKIGTP+PLFKELKDEEVELYRDKFAGSQAQRI
Sbjct: 541 VERHMSLCEEKGDIERAKQPWKILPSGHKIGTPEPLFKELKDEEVELYRDKFAGSQAQRI 600

Query: 601 VKAEAE--KLAAHLKKTNVSGSEKKQQAKSSSGRKPKAAAGGGAVEAGITITRLDIRVGL 660
           V+AEAE  KLAA LK TNVSGS KKQQAKS+ G K KAA     VE  ITITRLDIRVGL
Sbjct: 601 VRAEAEAEKLAAQLKSTNVSGSGKKQQAKST-GSKSKAA-----VEPEITITRLDIRVGL 660

Query: 661 ITKAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKPASMRGI 720
           ITKAQKHPDADSLYVEEIDVGE+QPRTVVSGLV YIPL+EMQNRKVCVLCNLKPA+MRGI
Sbjct: 661 ITKAQKHPDADSLYVEEIDVGESQPRTVVSGLVKYIPLEEMQNRKVCVLCNLKPAAMRGI 720

Query: 721 KSQAMVLAASNSDHTKVELVEPPKDANVGERIKFPGFEGEPEDVLNPKKKVWETLQVDLA 757
           KSQAMVLAASNSDHTKVELVEPPKDA VGERIKF GFEGEP+ VLNPKKKVWETLQVDLA
Sbjct: 721 KSQAMVLAASNSDHTKVELVEPPKDAIVGERIKFSGFEGEPDSVLNPKKKVWETLQVDLA 780

BLAST of Csor.00g257440 vs. ExPASy TrEMBL
Match: A0A6J1CBJ9 (Methionyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111009713 PE=3 SV=1)

HSP 1 Score: 1378 bits (3566), Expect = 0.0
Identity = 688/811 (84.83%), Postives = 717/811 (88.41%), Query Frame = 0

Query: 1   MADVLGDAGGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRL 60
           M DV GDAGGG TK TKLP+ GKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCR+
Sbjct: 1   MGDVSGDAGGGRTKVTKLPVAGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRI 60

Query: 61  RGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSS 120
           RGYNAIYICGTDEYGTATETKAMEEKC+PKEICDKY+ IHKEVY+WFGISFDEFGRTSS 
Sbjct: 61  RGYNAIYICGTDEYGTATETKAMEEKCTPKEICDKYYAIHKEVYNWFGISFDEFGRTSSP 120

Query: 121 QQTEVCQEIFGKLLENNWLSENTMLQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQC 180
           QQTEVCQEIFGKLLENNWLSENTM QLYCDTC+RFLADRLVEGICPTPGC+YDSARGDQC
Sbjct: 121 QQTEVCQEIFGKLLENNWLSENTMQQLYCDTCKRFLADRLVEGICPTPGCDYDSARGDQC 180

Query: 181 EKCGKLLNPTELKDPRCKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNA 240
           EKCGKLLNPTELKDPRCKVCQT+PRIRDT+HMFLELPLLR KLEEYINK SVAGSWSQNA
Sbjct: 181 EKCGKLLNPTELKDPRCKVCQTTPRIRDTDHMFLELPLLRAKLEEYINKTSVAGSWSQNA 240

Query: 241 IQATQAWLKEGLKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEK 300
           IQAT AWLKEGLKPRCITRDLKWGVPVPLE+FKDKVFYVWFDAPIGYVSITSCYTNEWEK
Sbjct: 241 IQATNAWLKEGLKPRCITRDLKWGVPVPLEKFKDKVFYVWFDAPIGYVSITSCYTNEWEK 300

Query: 301 WWKNPENVELFQFMGKDNVPFHTV------------------------------------ 360
           WWKNPENVELFQFMGKDNVPFHTV                                    
Sbjct: 301 WWKNPENVELFQFMGKDNVPFHTVMFPSTLLGTGESWTLMKTISVTEYLNYEAGKFSKSK 360

Query: 361 ----------------EVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVL 420
                           EVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVL
Sbjct: 361 GIGVFGNDAKDTNIPVEVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVL 420

Query: 421 SFIAKPLGQGYGSIIPDAPNADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQGLKAAMAI 480
           SFIAKPLGQGYGSIIPDAP+A+SHGLT+GLAEKVGKYVDQYVESMEKVKLKQGLKAAMAI
Sbjct: 421 SFIAKPLGQGYGSIIPDAPDAESHGLTMGLAEKVGKYVDQYVESMEKVKLKQGLKAAMAI 480

Query: 481 SSEGNTYLQTSQFWNLYKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFSDEVFRQLDLP 540
           SSEGNTYLQT+QFWNLYK D+PSCN+VMRTS GLVHLLACLLEPFMPSFS EVF++L+LP
Sbjct: 481 SSEGNTYLQTAQFWNLYKEDRPSCNVVMRTSAGLVHLLACLLEPFMPSFSLEVFKKLNLP 540

Query: 541 LERRISLCEEKGDIERAKQPWKTLPSGHKIGTPQPLFKELKDEEVELYRDKFAGSQAQRI 600
           +ER ISLCEEKGDIERAKQPWK LPSG KIG P+PLFKELKDEEVELYRDKFAGSQAQRI
Sbjct: 541 VERHISLCEEKGDIERAKQPWKILPSGLKIGIPEPLFKELKDEEVELYRDKFAGSQAQRI 600

Query: 601 VKAEAE--KLAAHLKKTNVSGSEKKQQAKSSSGRKPKAAAGGGAVEAGITITRLDIRVGL 660
           V+AEAE  KLAA LKKTNV+G  KKQQ KS+ G KPKAA     VE  ITITRLDIRVGL
Sbjct: 601 VRAEAEAEKLAAQLKKTNVAGGGKKQQTKST-GSKPKAA-----VEPEITITRLDIRVGL 660

Query: 661 ITKAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKPASMRGI 720
           ITKAQKHPDADSLYVEEIDVGE QPRTVVSGLV YIPL+EMQNRKVCVLCNLKPASMRGI
Sbjct: 661 ITKAQKHPDADSLYVEEIDVGEQQPRTVVSGLVKYIPLEEMQNRKVCVLCNLKPASMRGI 720

Query: 721 KSQAMVLAASNSDHTKVELVEPPKDANVGERIKFPGFEGEPEDVLNPKKKVWETLQVDLA 757
           KSQAMVLAASNSDHTKVELVEPP+DA VGER+KFPGFEGEP+DVLNPKKKVWETLQVDL 
Sbjct: 721 KSQAMVLAASNSDHTKVELVEPPQDAIVGERVKFPGFEGEPDDVLNPKKKVWETLQVDLG 780

BLAST of Csor.00g257440 vs. ExPASy TrEMBL
Match: A0A6J1D7S3 (Methionyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111017803 PE=3 SV=1)

HSP 1 Score: 1377 bits (3565), Expect = 0.0
Identity = 688/812 (84.73%), Postives = 716/812 (88.18%), Query Frame = 0

Query: 1   MADVLGDA-GGGGTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCR 60
           M  V GD    G TKATKLPI G+RN+LITSALPYVNNVPHLGNIIGCVLSADVFARYCR
Sbjct: 1   MGGVSGDTVAAGRTKATKLPIAGRRNVLITSALPYVNNVPHLGNIIGCVLSADVFARYCR 60

Query: 61  LRGYNAIYICGTDEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSS 120
           +RGYNAIY+CGTDEYGTATETKAMEEKC+P+EICDKYH IHKEVY+WFGISFDEFGRTSS
Sbjct: 61  IRGYNAIYVCGTDEYGTATETKAMEEKCTPQEICDKYHAIHKEVYNWFGISFDEFGRTSS 120

Query: 121 SQQTEVCQEIFGKLLENNWLSENTMLQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQ 180
            QQTEVCQ IFGKLLENNWLSENTM QLYCDTCERFLADRLVEGICPTPGC++DSARGDQ
Sbjct: 121 PQQTEVCQAIFGKLLENNWLSENTMQQLYCDTCERFLADRLVEGICPTPGCDHDSARGDQ 180

Query: 181 CEKCGKLLNPTELKDPRCKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQN 240
           CEKCGKLLNPTELKDPRCKVCQTSPRIRDTNHMFLELPLLRD+LEEYINKMSVAGSWSQN
Sbjct: 181 CEKCGKLLNPTELKDPRCKVCQTSPRIRDTNHMFLELPLLRDRLEEYINKMSVAGSWSQN 240

Query: 241 AIQATQAWLKEGLKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWE 300
           AIQAT AWLKEGLKPRCITRDLKWGVPVP+ERFKDKVFYVWFD PIGYVSITSCYTNEWE
Sbjct: 241 AIQATHAWLKEGLKPRCITRDLKWGVPVPVERFKDKVFYVWFDXPIGYVSITSCYTNEWE 300

Query: 301 KWWKNPENVELFQFMGKDNVPFHTV----------------------------------- 360
           KWWKNPENVELFQFMGKDNVPFHTV                                   
Sbjct: 301 KWWKNPENVELFQFMGKDNVPFHTVMFPSTLLGTGESWTLMKTINVTEYLNYEAGKFSKS 360

Query: 361 -----------------EVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRV 420
                            EVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRV
Sbjct: 361 KGVGVFGNDAKDTNIPVEVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRV 420

Query: 421 LSFIAKPLGQGYGSIIPDAPNADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQGLKAAMA 480
           LSFIAKPLGQGYGSIIPDAP+A+SHGLT GLAEKVGKYVDQYVESMEKVKLKQGLKAAMA
Sbjct: 421 LSFIAKPLGQGYGSIIPDAPDAESHGLTTGLAEKVGKYVDQYVESMEKVKLKQGLKAAMA 480

Query: 481 ISSEGNTYLQTSQFWNLYKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFSDEVFRQLDL 540
           ISSEGNTYLQT+QFWNLYK DKPSCN+VMRTS GLVHLLACLLEPFMPSFS EVF+QL+L
Sbjct: 481 ISSEGNTYLQTAQFWNLYKEDKPSCNVVMRTSAGLVHLLACLLEPFMPSFSLEVFKQLNL 540

Query: 541 PLERRISLCEEKGDIERAKQPWKTLPSGHKIGTPQPLFKELKDEEVELYRDKFAGSQAQR 600
           P E   SLCEEKGDIE+AKQPWK LPSGHKIGTP+PLFKELKDEEVE+YRDKFAGSQAQR
Sbjct: 541 PGEWHASLCEEKGDIEKAKQPWKILPSGHKIGTPEPLFKELKDEEVEIYRDKFAGSQAQR 600

Query: 601 IVKAEAE--KLAAHLKKTNVSGSEKKQQAKSSSGRKPKAAAGGGAVEAGITITRLDIRVG 660
           IV+AEAE  KLAA LKKTNVSG  KKQQAKS+ G KPKAA     VE  ITITRLDIRVG
Sbjct: 601 IVRAEAEAEKLAAQLKKTNVSGGGKKQQAKST-GSKPKAA-----VEPEITITRLDIRVG 660

Query: 661 LITKAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKPASMRG 720
           LITKAQKHPDADSLYVEEIDVGE QPRTVVSGLV YIPL+EMQNRKVCVLCNLKPASMRG
Sbjct: 661 LITKAQKHPDADSLYVEEIDVGEQQPRTVVSGLVKYIPLEEMQNRKVCVLCNLKPASMRG 720

Query: 721 IKSQAMVLAASNSDHTKVELVEPPKDANVGERIKFPGFEGEPEDVLNPKKKVWETLQVDL 757
           IKSQAMVLAASNSDHTKVELVEPPKDAN+GER+KFPGFEGEP+DVLNPKKK+WETLQVDL
Sbjct: 721 IKSQAMVLAASNSDHTKVELVEPPKDANIGERVKFPGFEGEPDDVLNPKKKIWETLQVDL 780

BLAST of Csor.00g257440 vs. TAIR 10
Match: AT4G13780.1 (methionine--tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS, putative )

HSP 1 Score: 1147.1 bits (2966), Expect = 0.0e+00
Identity = 564/798 (70.68%), Postives = 636/798 (79.70%), Query Frame = 0

Query: 12  GTKATKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYNAIYICGT 71
           G  + KLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADV+ARYCRLRGYNAIYICGT
Sbjct: 5   GKSSPKLPIPGKRNILITSALPYVNNVPHLGNIIGCVLSADVYARYCRLRGYNAIYICGT 64

Query: 72  DEYGTATETKAMEEKCSPKEICDKYHKIHKEVYDWFGISFDEFGRTSSSQQTEVCQEIFG 131
           DEYGTATETKA+EE C+PKEICDKYH IHKEVYDWFGISFD+FGRTS+ +QTEVCQ IF 
Sbjct: 65  DEYGTATETKALEENCTPKEICDKYHAIHKEVYDWFGISFDKFGRTSTPEQTEVCQAIFN 124

Query: 132 KLLENNWLSENTMLQLYCDTCERFLADRLVEGICPTPGCEYDSARGDQCEKCGKLLNPTE 191
           KL +N WLSENTM QLYCDTC++FLADRLVEG CP  GC YDSARGDQCEKCGKLLNPTE
Sbjct: 125 KLWDNKWLSENTMQQLYCDTCKKFLADRLVEGSCPFEGCNYDSARGDQCEKCGKLLNPTE 184

Query: 192 LKDPRCKVCQTSPRIRDTNHMFLELPLLRDKLEEYINKMSVAGSWSQNAIQATQAWLKEG 251
           LKDP+CKVCQ +PRIRDT+H+F+ELPLL+D+LE YI K SV GSWSQNAIQ T AWL++G
Sbjct: 185 LKDPKCKVCQNTPRIRDTDHLFIELPLLKDRLEAYIKKTSVTGSWSQNAIQTTNAWLRDG 244

Query: 252 LKPRCITRDLKWGVPVPLERFKDKVFYVWFDAPIGYVSITSCYTNEWEKWWKNPENVELF 311
           L+ RCITRDLKWGVPVP E++KDKVFYVWFDAPIGYVSITSCYT+EWEKWWKNPENVEL+
Sbjct: 245 LRQRCITRDLKWGVPVPHEKYKDKVFYVWFDAPIGYVSITSCYTSEWEKWWKNPENVELY 304

Query: 312 QFMGKDNVPFHT------------------------------------------------ 371
           QFMGKDNVPFHT                                                
Sbjct: 305 QFMGKDNVPFHTVMFPSTQLGTEENWTLMKTISVTEYLNYEDGKFSKSKGVGVFGNDVKD 364

Query: 372 ----VEVWRYYLLANRPEVSDTLFTWADLQAKLNGELLNNLGNFIHRVLSFIAKPLGQGY 431
               VEVWRYYLL NRPEVSDT F+W DLQAKLNGELL+NLGNF++RVLSFIAKP   GY
Sbjct: 365 TNIPVEVWRYYLLTNRPEVSDTSFSWTDLQAKLNGELLSNLGNFVNRVLSFIAKPDNAGY 424

Query: 432 GSIIPDAPNADSHGLTLGLAEKVGKYVDQYVESMEKVKLKQGLKAAMAISSEGNTYLQTS 491
           GS+IPDA +A+SH LT  LAEKV K+V +YVE+MEKVKLKQGLK AM ISSEGN YLQ S
Sbjct: 425 GSVIPDAHDAESHSLTKSLAEKVEKFVAEYVEAMEKVKLKQGLKTAMLISSEGNYYLQAS 484

Query: 492 QFWNLYKADKPSCNIVMRTSTGLVHLLACLLEPFMPSFSDEVFRQLDLPLERRISLCEEK 551
           QFW LYK DKP C +V+RT+ GLVHLLA LLEPFMPSFS EVF+QL+LP   + SL +E+
Sbjct: 485 QFWKLYKEDKPLCAVVIRTAAGLVHLLAQLLEPFMPSFSCEVFKQLNLP--PQFSLSDER 544

Query: 552 GDIERAKQPWKTLPSGHKIGTPQPLFKELKDEEVELYRDKFAGSQAQRIVKAEAEKLAAH 611
           G++  A +PW  LP  H+IGTPQPLFKEL+++EV  YR+KFAGSQ+ R  + EA  LA  
Sbjct: 545 GEVLLASRPWDILPPSHRIGTPQPLFKELENDEVARYREKFAGSQSDRRARDEAANLADQ 604

Query: 612 LKKTNVSGSEKKQQAKSSSGRKPKAAAGGGAVEAGITITRLDIRVGLITKAQKHPDADSL 671
           L KT +S + KKQ+A S  G KPK      A +  IT+ RLDIRVG I KA+KHP AD+L
Sbjct: 605 LNKTKLSDA-KKQKASSKGGGKPKPQP---AADREITMARLDIRVGKIVKAEKHPKADAL 664

Query: 672 YVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKPASMRGIKSQAMVLAASNSD 731
           YVEEIDVG  + RTVVSGLV YIPL+EMQNR VCVLCNLKPA MR I SQAMVLAAS+SD
Sbjct: 665 YVEEIDVGGGEIRTVVSGLVKYIPLEEMQNRMVCVLCNLKPAKMRDIVSQAMVLAASSSD 724

Query: 732 HTKVELVEPPKDANVGERIKFPGFEGEPEDVLNPKKKVWETLQVDLAINTEGVACFKDIP 758
            +KVELVEPPK AN+GER+ FPGFEGEP+DVLNPKKKVWETL VDL      VAC+KD+P
Sbjct: 725 GSKVELVEPPKTANIGERVTFPGFEGEPDDVLNPKKKVWETLLVDLNTKENLVACYKDVP 784

BLAST of Csor.00g257440 vs. TAIR 10
Match: AT2G40660.1 (Nucleic acid-binding, OB-fold-like protein )

HSP 1 Score: 156.4 bits (394), Expect = 9.3e-38
Identity = 92/206 (44.66%), Postives = 124/206 (60.19%), Query Frame = 0

Query: 550 KAEAEKLAAHLKKTNVSGSEKKQQAKSSSGRKPKAAAGGGAVEAGITITRLDIRVGLITK 609
           K E E+   +  K   +  EKK+ A+    +K          EA ++++ L+I+VGLI K
Sbjct: 192 KTEPEEPKKNAAKEKDAKKEKKKPAEPEPAKK----------EAELSVSLLNIQVGLIRK 251

Query: 610 AQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVLCNLKPASMRGIKSQ 669
           A KHP ADSL VEEIDVGE + R VVSGL  +   +++ NR V ++ N+KP  +R + SQ
Sbjct: 252 AWKHPSADSLLVEEIDVGEDKVRQVVSGLAKFCSPEDLTNRLVALITNVKPGKLRDVMSQ 311

Query: 670 AMVLAASNSDHTKVELVEPPKDANVGERIKFPGFEGEPEDVLNPKKKVWETLQVDLAINT 729
            +VL AS+ DH+ VE + PP  A  GER+ F G EG+PEDVLNPKKK  E +   L  + 
Sbjct: 312 GLVLCASSEDHSVVEPLLPPAGAKPGERVSFSGIEGKPEDVLNPKKKQLEKITPGLYTDE 371

Query: 730 EGVACFKDIPFNTSAGIC--KVPSIT 754
            GVA +K I F TSAG C   +P  T
Sbjct: 372 NGVATYKGIQFMTSAGPCTSSIPKAT 387

BLAST of Csor.00g257440 vs. TAIR 10
Match: AT3G59980.1 (Nucleic acid-binding, OB-fold-like protein )

HSP 1 Score: 140.2 bits (352), Expect = 6.9e-33
Identity = 77/169 (45.56%), Postives = 108/169 (63.91%), Query Frame = 0

Query: 596 TITRLDIRVGLITKAQKHPDADSLYVEEIDVGEAQPRTVVSGLVNYIPLQEMQNRKVCVL 655
           T   LDI+VG I KA +H +ADSLYVEE+D+GEA+PR + SGLV Y+PL  +Q   V VL
Sbjct: 105 TANLLDIKVGRIVKAWQHEEADSLYVEEVDIGEAEPRIICSGLVKYVPLDLLQGASVVVL 164

Query: 656 CNLKPASMRGIKSQAMVLAASNSDHTKVELVEPPKDANVGERIKFPGFEGEPEDVLNP-- 715
            NLKP +MRG+KS  M+LAAS++ H  VEL+ PP+ +  G+R+ F G E + E +  P  
Sbjct: 165 ANLKPRNMRGVKSCGMLLAASDAAHENVELLVPPEGSVPGDRVWF-GNEEDLEQLPEPAP 224

Query: 716 -----KKKVWETLQVDLAINTEGVACFKDIPFNTSAGICKVPSITSGSI 758
                KKK+WE +Q  L  +  GV+  K+    TS+G+    S+ + +I
Sbjct: 225 PNKVQKKKMWELVQPLLKTDASGVSMLKEHLMRTSSGLVTSKSLRNANI 272

BLAST of Csor.00g257440 vs. TAIR 10
Match: AT5G02680.1 (LOCATED IN: endomembrane system; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: methionine--tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS, putative (TAIR:AT4G13780.1); Has 252 Blast hits to 252 proteins in 89 species: Archae - 0; Bacteria - 71; Metazoa - 93; Fungi - 0; Plants - 74; Viruses - 0; Other Eukaryotes - 14 (source: NCBI BLink). )

HSP 1 Score: 62.8 bits (151), Expect = 1.4e-09
Identity = 29/36 (80.56%), Postives = 31/36 (86.11%), Query Frame = 0

Query: 338 VSDTLFTWADLQAKLNGELLNNLGNFIHRVLSFIAK 374
           VSDTLF W DLQAKLNGE LNNLGN ++R LSFIAK
Sbjct: 87  VSDTLFPWNDLQAKLNGEFLNNLGNSVNRALSFIAK 122

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZTS10.0e+0070.43Probable methionine--tRNA ligase OS=Oryza sativa subsp. japonica OX=39947 GN=Os0... [more]
Q9SVN50.0e+0070.68Methionine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At4g1378... [more]
Q209702.8e-18841.85Methionine--tRNA ligase, cytoplasmic OS=Caenorhabditis elegans OX=6239 GN=mars-1... [more]
Q54X956.2e-18043.34Probable methionine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=446... [more]
Q68FL69.9e-16247.97Methionine--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Mars1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6574842.10.0100.00Methionine--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_022958743.10.092.84probable methionine--tRNA ligase [Cucurbita moschata][more]
KAG7013416.10.090.66putative methionine--tRNA ligase [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023006265.10.091.65probable methionine--tRNA ligase [Cucurbita maxima][more]
XP_023548158.10.091.49methionine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1H3Y00.092.84Methionyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111459931 PE=3 SV=... [more]
A0A6J1KZN80.091.65Methionyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111499054 PE=3 SV=1[more]
A0A6J1KIY20.085.33Methionyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111494895 PE=3 SV=1[more]
A0A6J1CBJ90.084.83Methionyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111009713 PE=3 SV... [more]
A0A6J1D7S30.084.73Methionyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111017803 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT4G13780.10.0e+0070.68methionine--tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS,... [more]
AT2G40660.19.3e-3844.66Nucleic acid-binding, OB-fold-like protein [more]
AT3G59980.16.9e-3345.56Nucleic acid-binding, OB-fold-like protein [more]
AT5G02680.11.4e-0980.56LOCATED IN: endomembrane system; EXPRESSED IN: 21 plant structures; EXPRESSED DU... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR033911Methioninyl-tRNA synthetase core domainPRINTSPR01041TRNASYNTHMETcoord: 277..288
score: 68.52
coord: 109..120
score: 49.69
coord: 28..41
score: 58.99
coord: 312..327
score: 42.13
coord: 61..75
score: 50.62
IPR033911Methioninyl-tRNA synthetase core domainCDDcd00814MetRS_corecoord: 25..343
e-value: 4.04387E-130
score: 387.273
NoneNo IPR availableGENE3D2.40.50.140coord: 596..758
e-value: 9.4E-64
score: 216.3
NoneNo IPR availableGENE3D1.10.730.10coord: 346..539
e-value: 2.6E-45
score: 156.2
NoneNo IPR availableGENE3D2.170.220.10coord: 120..277
e-value: 3.9E-130
score: 435.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 560..588
NoneNo IPR availablePANTHERPTHR45765METHIONINE--TRNA LIGASEcoord: 13..324
NoneNo IPR availablePANTHERPTHR45765:SF4METHIONINE--TRNA LIGASE CYTOPLASMICcoord: 13..324
NoneNo IPR availablePANTHERPTHR45765:SF4METHIONINE--TRNA LIGASE CYTOPLASMICcoord: 325..757
NoneNo IPR availablePANTHERPTHR45765METHIONINE--TRNA LIGASEcoord: 325..757
NoneNo IPR availableCDDcd02799tRNA_bind_EMAP-II_likecoord: 595..698
e-value: 2.52062E-62
score: 202.073
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 23..353
IPR014758Methionyl-tRNA synthetaseTIGRFAMTIGR00398TIGR00398coord: 26..324
e-value: 1.6E-116
score: 388.1
IPR015413Methionyl/Leucyl tRNA synthetasePFAMPF09334tRNA-synt_1gcoord: 26..324
e-value: 3.2E-118
score: 394.9
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 27..332
e-value: 3.9E-130
score: 435.8
IPR002547tRNA-binding domainPFAMPF01588tRNA_bindcoord: 602..696
e-value: 7.8E-33
score: 112.5
IPR002547tRNA-binding domainPROSITEPS50886TRBDcoord: 596..699
score: 31.925245
IPR001412Aminoacyl-tRNA synthetase, class I, conserved sitePROSITEPS00178AA_TRNA_LIGASE_Icoord: 33..44
IPR041872Methionyl-tRNA synthetase, anticodon-binding domainCDDcd07957Anticodon_Ia_Metcoord: 352..485
e-value: 2.0572E-35
score: 128.376
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 598..756
IPR029038Methionyl-tRNA synthetase, Zn-domainSUPERFAMILY57770Methionyl-tRNA synthetase (MetRS), Zn-domaincoord: 161..197
IPR009080Aminoacyl-tRNA synthetase, class Ia, anticodon-bindingSUPERFAMILY47323Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetasescoord: 360..540

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g257440.m01Csor.00g257440.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006431 methionyl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005829 cytosol
molecular_function GO:0005524 ATP binding
molecular_function GO:0004825 methionine-tRNA ligase activity
molecular_function GO:0000049 tRNA binding
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding