Csor.00g257320 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g257320
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationCsor_Chr17: 367865 .. 368983 (+)
RNA-Seq ExpressionCsor.00g257320
SyntenyCsor.00g257320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codonsinglepolypeptidestop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACTCCGTCGGAATCCCCGATCCCTCCCCGCCGTTCAATCCGTTCAACTTTAACGACTGTTCTCAAGAACTTTGCACAGCCTTCTGTCCGCAGTGGTGCGCCTTACCGTTCCCACCGCCGCCGCTCTTCGGTCTCGACGACAGTGACGATTCCGATACATTCTTCTCGCCTCTAATAGTCGCGGTGATCGGGATCTTAGCTAGCGCGTTTGTTCTGGTTACTTACTACGCGATCGTGTCGAAATATTGCCGGCGGAGAGTCGACGATGGCGGGGGAGCCGAACCGGTGGAGAACTTCGAGGCGGATCGAGTGGTTAACGATACTCGGCAGTCGAGTGCGGGACCGGGATTGAATGAGGCGCTGTTAAAATCGATCTCAGTTTTCAAGTTCAAGAAGGGCGAAGGATTGATCGAAGAAAGCGATTGCGCTATATGTTTGAGTGAATTTCAAGAGAACGAGAGTTTGCGATTGTTGCCTAAATGTAGTCACGCTTTTCATCTCGCGTGTATTGATACTTGGCTGAAATCCAATTCGAGTTGTCCTTTCTGTCGTTCCAATATAACGCCGACGAATCCTCCGCCTCCGGAAACTCCGCCGCCATCCGTGACGGTTCTAGGTTACCGCCACAGCAGCGACGCTGCCGTCGTCGTCGTCCAAGATTTGTCCGAAAACGTAAGCCAGGAGGTCGTCGTGGTCGAAATCGTAACCGATTCAACCAATCGAGATCAAGGAAGTCCGAATCCGAATCCGAATCCAAATCCGCCGAGTGTCGATCGAGGAGACGGAAGAACAGAGAGAAACAGAGAAGAATCCATCCGTTCGATTCGGTCAGTTTCAGAGGATCATCAGTCTTCTCCATGTCCAGTCCGCATTCTCTCTGTTGCAGATATATTGAGCATCAACGAGGAAGAAGACGAAGAAGTTGAAGCTATGAATTCATCCATGGCGGCCGGTACTGGTCCGTCAAACAACGACGGCAAAGAAACAGAAAAATCCAGAAACAGAAACAGCGTTTTGAATTCCGCCATGAGAATGTCGATTCCATCTGGAATTTTTTCGTTCCAAAGGTATGCAAAAGGGAAATGTTCGAATTTACATCAAAATTTTCAGAACTAA

mRNA sequence

ATGGACTCCGTCGGAATCCCCGATCCCTCCCCGCCGTTCAATCCGTTCAACTTTAACGACTGTTCTCAAGAACTTTGCACAGCCTTCTGTCCGCAGTGGTGCGCCTTACCGTTCCCACCGCCGCCGCTCTTCGGTCTCGACGACAGTGACGATTCCGATACATTCTTCTCGCCTCTAATAGTCGCGGTGATCGGGATCTTAGCTAGCGCGTTTGTTCTGGTTACTTACTACGCGATCGTGTCGAAATATTGCCGGCGGAGAGTCGACGATGGCGGGGGAGCCGAACCGGTGGAGAACTTCGAGGCGGATCGAGTGGTTAACGATACTCGGCAGTCGAGTGCGGGACCGGGATTGAATGAGGCGCTGTTAAAATCGATCTCAGTTTTCAAGTTCAAGAAGGGCGAAGGATTGATCGAAGAAAGCGATTGCGCTATATGTTTGAGTGAATTTCAAGAGAACGAGAGTTTGCGATTGTTGCCTAAATGTAGTCACGCTTTTCATCTCGCGTGTATTGATACTTGGCTGAAATCCAATTCGAGTTGTCCTTTCTGTCGTTCCAATATAACGCCGACGAATCCTCCGCCTCCGGAAACTCCGCCGCCATCCGTGACGGTTCTAGGTTACCGCCACAGCAGCGACGCTGCCGTCGTCGTCGTCCAAGATTTGTCCGAAAACGTAAGCCAGGAGGTCGTCGTGGTCGAAATCGTAACCGATTCAACCAATCGAGATCAAGGAAGTCCGAATCCGAATCCGAATCCAAATCCGCCGAGTGTCGATCGAGGAGACGGAAGAACAGAGAGAAACAGAGAAGAATCCATCCGTTCGATTCGGTCAGTTTCAGAGGATCATCAGTCTTCTCCATGTCCAGTCCGCATTCTCTCTGTTGCAGATATATTGAGCATCAACGAGGAAGAAGACGAAGAAGTTGAAGCTATGAATTCATCCATGGCGGCCGGTACTGGTCCGTCAAACAACGACGGCAAAGAAACAGAAAAATCCAGAAACAGAAACAGCGTTTTGAATTCCGCCATGAGAATGTCGATTCCATCTGGAATTTTTTCGTTCCAAAGGTATGCAAAAGGGAAATGTTCGAATTTACATCAAAATTTTCAGAACTAA

Coding sequence (CDS)

ATGGACTCCGTCGGAATCCCCGATCCCTCCCCGCCGTTCAATCCGTTCAACTTTAACGACTGTTCTCAAGAACTTTGCACAGCCTTCTGTCCGCAGTGGTGCGCCTTACCGTTCCCACCGCCGCCGCTCTTCGGTCTCGACGACAGTGACGATTCCGATACATTCTTCTCGCCTCTAATAGTCGCGGTGATCGGGATCTTAGCTAGCGCGTTTGTTCTGGTTACTTACTACGCGATCGTGTCGAAATATTGCCGGCGGAGAGTCGACGATGGCGGGGGAGCCGAACCGGTGGAGAACTTCGAGGCGGATCGAGTGGTTAACGATACTCGGCAGTCGAGTGCGGGACCGGGATTGAATGAGGCGCTGTTAAAATCGATCTCAGTTTTCAAGTTCAAGAAGGGCGAAGGATTGATCGAAGAAAGCGATTGCGCTATATGTTTGAGTGAATTTCAAGAGAACGAGAGTTTGCGATTGTTGCCTAAATGTAGTCACGCTTTTCATCTCGCGTGTATTGATACTTGGCTGAAATCCAATTCGAGTTGTCCTTTCTGTCGTTCCAATATAACGCCGACGAATCCTCCGCCTCCGGAAACTCCGCCGCCATCCGTGACGGTTCTAGGTTACCGCCACAGCAGCGACGCTGCCGTCGTCGTCGTCCAAGATTTGTCCGAAAACGTAAGCCAGGAGGTCGTCGTGGTCGAAATCGTAACCGATTCAACCAATCGAGATCAAGGAAGTCCGAATCCGAATCCGAATCCAAATCCGCCGAGTGTCGATCGAGGAGACGGAAGAACAGAGAGAAACAGAGAAGAATCCATCCGTTCGATTCGGTCAGTTTCAGAGGATCATCAGTCTTCTCCATGTCCAGTCCGCATTCTCTCTGTTGCAGATATATTGAGCATCAACGAGGAAGAAGACGAAGAAGTTGAAGCTATGAATTCATCCATGGCGGCCGGTACTGGTCCGTCAAACAACGACGGCAAAGAAACAGAAAAATCCAGAAACAGAAACAGCGTTTTGAATTCCGCCATGAGAATGTCGATTCCATCTGGAATTTTTTCGTTCCAAAGGTATGCAAAAGGGAAATGTTCGAATTTACATCAAAATTTTCAGAACTAA

Protein sequence

MDSVGIPDPSPPFNPFNFNDCSQELCTAFCPQWCALPFPPPPLFGLDDSDDSDTFFSPLIVAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVVNDTRQSSAGPGLNEALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSSCPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDSTNRDQGSPNPNPNPNPPSVDRGDGRTERNREESIRSIRSVSEDHQSSPCPVRILSVADILSINEEEDEEVEAMNSSMAAGTGPSNNDGKETEKSRNRNSVLNSAMRMSIPSGIFSFQRYAKGKCSNLHQNFQN
Homology
BLAST of Csor.00g257320 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 7.3e-57
Identity = 144/372 (38.71%), Postives = 210/372 (56.45%), Query Frame = 0

Query: 7   PDPSPPFNPFNFNDCSQELCTAFCPQWCALPFPPPP--LFGLDDSDDSDTFFSPLIVAVI 66
           P+P  P++  ++NDCSQ +C  +CPQWC L FPPPP   F  DDS  S + FSPL++A+I
Sbjct: 5   PNPWSPYD--SYNDCSQGICNIYCPQWCYLIFPPPPPSFFLDDDSSSSSSSFSPLLIALI 64

Query: 67  GILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVVNDT-RQSSAGPGLNEALL 126
           GIL SA +LV+YY ++SKYC R            N   +   + T R S+ G GLNE+++
Sbjct: 65  GILTSALILVSYYTLISKYCHRHHQTSSSETLNLNHNGEGFFSSTQRISTNGDGLNESMI 124

Query: 127 KSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSSCPF 186
           KSI+V+K+K G+G ++ SDC++CLSEF+ENESLRLLPKC+HAFHL CIDTWLKS+S+CP 
Sbjct: 125 KSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHSNCPL 184

Query: 187 CRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVS----------QEVVVV 246
           CR+ +T  N P          V+  + +S      V +++ N++            VV+ 
Sbjct: 185 CRAFVTGVNNPTASVGQNVSVVVANQSNSAHQTGSVSEINLNLAGYESQTGDFDSVVVIE 244

Query: 247 EIVTDSTNRDQGSPNPNPNPNPPSVDRGDGRTERNREESIRSIRSVSEDHQSSPCPVRIL 306
           ++   S N D  S    P          + R E   E+S+   RSVS +         ++
Sbjct: 245 DLEIGSRNSDARSELQLP----------EERRETKDEDSLPIRRSVSLNSGV------VV 304

Query: 307 SVADILSINEEEDEEVEAMNSSMAAGTGPSNNDGK---ETEKSRNRNSVLNSAMRMSIPS 363
           S+AD+L   E+E+ E   + +S     G  + DGK    TE ++    V    +R S+ +
Sbjct: 305 SIADVLREIEDEEGESGGVGTSQRREEG-EDGDGKTIPPTEANQRSGGVSGFFVR-SLST 356

BLAST of Csor.00g257320 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 211.1 bits (536), Expect = 2.2e-53
Identity = 139/367 (37.87%), Postives = 200/367 (54.50%), Query Frame = 0

Query: 1   MDSVGIPDPSPPFNPFNFNDCSQELCTAFCPQWCALPFPPPPLFGLDDSDDSDTF-FSPL 60
           M S G P+P       ++ DCSQ +C+ +CPQWC + FPPPP F LDD DDS +  FSPL
Sbjct: 1   MGSTGNPNPWGTTYD-SYRDCSQGVCSVYCPQWCYVIFPPPPSFYLDDEDDSSSSDFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRRVDDGG--GAEPVENFEADRV------------ 120
           ++A+IGILASAF+LV+YY ++SKYC RR  +     A  +    +D              
Sbjct: 61  LIALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNNNNNGA 120

Query: 121 --VNDTRQSSAGPGLNEALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCS 180
              N T     G GL+E+L+KSI+V+K++K +G +E SDC++CLSEFQENESLRLLPKC+
Sbjct: 121 TNPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCN 180

Query: 181 HAFHLACIDTWLKSNSSCPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLS 240
           HAFH+ CIDTWLKS+S+CP CR+ I  ++         +V ++     ++  +V   +  
Sbjct: 181 HAFHVPCIDTWLKSHSNCPLCRAFIVTSS---------AVEIVDL---TNQQIVTENNSI 240

Query: 241 ENVSQEVVVVEIVTDSTNRDQGSPNPNPNPNPPSV-DRGDGRTERNREESIRSIRSVSED 300
                 VVVV +  +++     + N    P PP + D  DG  E  R  S+ S   V   
Sbjct: 241 STGDDSVVVVNLDLENSRSRNETVNEGSTPKPPEMQDSRDG--EERRSASLNSGGGV--- 300

Query: 301 HQSSPCPVRILSVADILSINEEEDEEVEAMNSSMAAGTGPSNNDGKETEKSRNR-NSVLN 349
                     +S+ADIL    E+DEE   + +S     G        +  + N+ N + N
Sbjct: 301 ----------VSIADILR-EIEDDEESAGVGTSRWVEEGEGEKTPPPSGSAANQTNGISN 338

BLAST of Csor.00g257320 vs. ExPASy Swiss-Prot
Match: Q7XLY8 (E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0590900 PE=2 SV=2)

HSP 1 Score: 184.1 bits (466), Expect = 2.9e-45
Identity = 131/351 (37.32%), Postives = 178/351 (50.71%), Query Frame = 0

Query: 12  PFNPFNFNDCSQELCTAFCPQWCALPFPPPPLF---GLDDSDDSDTFFSPLIVAVIGILA 71
           P+ P    DCSQ LC+ +CPQWC   FPPPP F   G    D S   FSPL++A+IG+LA
Sbjct: 10  PYEP--TRDCSQGLCSMYCPQWCYFIFPPPPPFDVAGTSADDSSGPVFSPLVIAIIGVLA 69

Query: 72  SAFVLVTYYAIVSKYC------RRRV---------DDGGGAEPVENFEADRVVNDTRQSS 131
           SAF+LV+YY  +SKYC      R RV           GG      +       +++   S
Sbjct: 70  SAFLLVSYYTFISKYCGTVSSLRGRVFGSSSGGAAYGGGAGSGGRHGHGQSRSHESWNVS 129

Query: 132 AGPGLNEALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDT 191
              GL+E L+  I+V K+++G+G +  +DC++CL EF + ESLRLLP+CSHAFH  CIDT
Sbjct: 130 PPSGLDETLINKITVCKYRRGDGFVHTTDCSVCLGEFSDGESLRLLPRCSHAFHQQCIDT 189

Query: 192 WLKSNSSCPFCRSNITPTN----PPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQE 251
           WLKS+S+CP CR+NIT        P PE   P  T     H     VVVV D  EN+ +E
Sbjct: 190 WLKSHSNCPLCRANITFVTVGLASPEPEGCAPGETGGDNTHE----VVVVMDGLENLCEE 249

Query: 252 VVVVEIVTDSTNRDQGSPNPNPNPNPPSVDRGDGRTERNREESIRSIRSVSEDHQSSPCP 311
               E V+ ++  D          +  + D  +G  E N    IR   S  +   SS   
Sbjct: 250 --QQEAVSRASTADD---------DHDAKDVAEGMEEANGAAEIREEGSPPKRGASSFDL 309

Query: 312 VR--ILSVADILSINEEEDEEVEAMNSSMAAGTGPSNNDGKETEKSRNRNS 339
            R   + +AD+L  + E++      +  +A G G S     E  K R   S
Sbjct: 310 HRDNRMCIADVLQESMEDELTAARESGLLAGGAGTSRRCHGENSKGRGGRS 343

BLAST of Csor.00g257320 vs. ExPASy Swiss-Prot
Match: P0C041 (Putative RING-H2 finger protein ATL53 OS=Arabidopsis thaliana OX=3702 GN=ATL53 PE=3 SV=2)

HSP 1 Score: 160.2 bits (404), Expect = 4.4e-38
Identity = 99/243 (40.74%), Postives = 134/243 (55.14%), Query Frame = 0

Query: 7   PDPSPPFNPFNFNDCSQELCTAFCPQWCA-LPFPPPPLF---GLDDSDDSDTFFSPLIVA 66
           P+PS      N  DC+Q LC+ FCPQWC  + F PPP+     L+D   S+   SPL++A
Sbjct: 5   PNPSAWNQYINPRDCTQGLCSTFCPQWCTYINFSPPPISYEQFLNDGVASNPNLSPLVIA 64

Query: 67  VIGILASAFVLVTYYAIVSKYCRRRVDDGGGAE--------PVENFEADRVVNDTRQSSA 126
           + GI A+AF+L  YY +VSKYC     +   +E         V + E     +     S+
Sbjct: 65  IFGIFATAFLLAAYYTLVSKYCANDTTNEAASESGRSDIILDVNSPERGDQDDPFALESS 124

Query: 127 GPGLNEALLKSISVFKFKKGEG--LIEESDCAICLSEFQENESLRLLPKCSHAFHLACID 186
             GL++ L+K I  FK KK +    I  +DC+ICL EF E+ESLRLLPKC+H FH+ CID
Sbjct: 125 TAGLDDTLIKKIGFFKLKKHQNGFKINGTDCSICLGEFNEDESLRLLPKCNHTFHVVCID 184

Query: 187 TWLKSNSSCPFCRSNITPTNPPPPETPPPSVTVLGY-RHSSDAAV----VVVQDLSENVS 231
            WLKS+S+CP CR+ I     P  + P   V V+   R +S+       VVV D  E VS
Sbjct: 185 RWLKSHSNCPLCRAKII---VPTTQQPEHHVVVMNLDRFTSNVGSAEGNVVVDDHREEVS 244

BLAST of Csor.00g257320 vs. ExPASy Swiss-Prot
Match: Q9LSW9 (RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 1.0e-26
Identity = 77/188 (40.96%), Postives = 110/188 (58.51%), Query Frame = 0

Query: 37  PFPPPPLFGLDDSDDSDTFFSPLIVAVIGILASAFVLVTYYAIVSKYCR--RRVDDGGG- 96
           P PPPP+F    +  + T F  L VAVIGILA+AF+LV+YY  V K C    R+D  G  
Sbjct: 17  PPPPPPIF--HRASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRIDILGRF 76

Query: 97  --AEPVENFEADRVVNDTRQSSAGPGLNEALLKSISVFKFKK----------GEGLIEE- 156
             +    N +   +V      S   GL+E+++++I +FKFKK          GEG  EE 
Sbjct: 77  SLSRRRRNDQDPLMVYSPELRSR--GLDESVIRAIPIFKFKKRYDQNDGVFTGEGEEEEE 136

Query: 157 ---SDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSSCPFCRSNIT-PTNPPPP 205
               +C++CLSEFQ+ E LR++P CSH FH+ CID WL++N++CP CR+ ++  T+ PP 
Sbjct: 137 KRSQECSVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRVSCDTSFPPD 196

BLAST of Csor.00g257320 vs. NCBI nr
Match: KAG6574830.1 (RING-H2 finger protein ATL52, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 741 bits (1914), Expect = 6.72e-270
Identity = 372/372 (100.00%), Postives = 372/372 (100.00%), Query Frame = 0

Query: 1   MDSVGIPDPSPPFNPFNFNDCSQELCTAFCPQWCALPFPPPPLFGLDDSDDSDTFFSPLI 60
           MDSVGIPDPSPPFNPFNFNDCSQELCTAFCPQWCALPFPPPPLFGLDDSDDSDTFFSPLI
Sbjct: 1   MDSVGIPDPSPPFNPFNFNDCSQELCTAFCPQWCALPFPPPPLFGLDDSDDSDTFFSPLI 60

Query: 61  VAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVVNDTRQSSAGPGLNE 120
           VAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVVNDTRQSSAGPGLNE
Sbjct: 61  VAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVVNDTRQSSAGPGLNE 120

Query: 121 ALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSS 180
           ALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSS
Sbjct: 121 ALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSS 180

Query: 181 CPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDST 240
           CPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDST
Sbjct: 181 CPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDST 240

Query: 241 NRDQGSPNPNPNPNPPSVDRGDGRTERNREESIRSIRSVSEDHQSSPCPVRILSVADILS 300
           NRDQGSPNPNPNPNPPSVDRGDGRTERNREESIRSIRSVSEDHQSSPCPVRILSVADILS
Sbjct: 241 NRDQGSPNPNPNPNPPSVDRGDGRTERNREESIRSIRSVSEDHQSSPCPVRILSVADILS 300

Query: 301 INEEEDEEVEAMNSSMAAGTGPSNNDGKETEKSRNRNSVLNSAMRMSIPSGIFSFQRYAK 360
           INEEEDEEVEAMNSSMAAGTGPSNNDGKETEKSRNRNSVLNSAMRMSIPSGIFSFQRYAK
Sbjct: 301 INEEEDEEVEAMNSSMAAGTGPSNNDGKETEKSRNRNSVLNSAMRMSIPSGIFSFQRYAK 360

Query: 361 GKCSNLHQNFQN 372
           GKCSNLHQNFQN
Sbjct: 361 GKCSNLHQNFQN 372

BLAST of Csor.00g257320 vs. NCBI nr
Match: XP_022958842.1 (RING-H2 finger protein ATL52-like [Cucurbita moschata])

HSP 1 Score: 738 bits (1906), Expect = 1.11e-268
Identity = 370/372 (99.46%), Postives = 371/372 (99.73%), Query Frame = 0

Query: 1   MDSVGIPDPSPPFNPFNFNDCSQELCTAFCPQWCALPFPPPPLFGLDDSDDSDTFFSPLI 60
           MDSVGIPDPSPPFNPFNFNDCSQELCTAFCPQWCALPFPPPPLFGLDDSDDSDTFFSPLI
Sbjct: 1   MDSVGIPDPSPPFNPFNFNDCSQELCTAFCPQWCALPFPPPPLFGLDDSDDSDTFFSPLI 60

Query: 61  VAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVVNDTRQSSAGPGLNE 120
           VAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVVNDTRQSSAGPGLNE
Sbjct: 61  VAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVVNDTRQSSAGPGLNE 120

Query: 121 ALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSS 180
           ALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSS
Sbjct: 121 ALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSS 180

Query: 181 CPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDST 240
           CPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDST
Sbjct: 181 CPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDST 240

Query: 241 NRDQGSPNPNPNPNPPSVDRGDGRTERNREESIRSIRSVSEDHQSSPCPVRILSVADILS 300
           NRDQGSPNPNPNPNPPSVDRGDGRTERNREESIRSIRSVSEDHQSSPCPVRILSVADILS
Sbjct: 241 NRDQGSPNPNPNPNPPSVDRGDGRTERNREESIRSIRSVSEDHQSSPCPVRILSVADILS 300

Query: 301 INEEEDEEVEAMNSSMAAGTGPSNNDGKETEKSRNRNSVLNSAMRMSIPSGIFSFQRYAK 360
           INEEEDEEVEAMNSSMAAG+GPSNNDGKETEKSRNRNSVLNSAMRMSIPSGIFSFQRY K
Sbjct: 301 INEEEDEEVEAMNSSMAAGSGPSNNDGKETEKSRNRNSVLNSAMRMSIPSGIFSFQRYVK 360

Query: 361 GKCSNLHQNFQN 372
           GKCSNLHQNFQN
Sbjct: 361 GKCSNLHQNFQN 372

BLAST of Csor.00g257320 vs. NCBI nr
Match: XP_023547750.1 (RING-H2 finger protein ATL52-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 717 bits (1851), Expect = 2.32e-260
Identity = 363/372 (97.58%), Postives = 365/372 (98.12%), Query Frame = 0

Query: 1   MDSVGIPDPSPPFNPFNFNDCSQELCTAFCPQWCALPFPPPPLFGLDDSDDSDTFFSPLI 60
           MDSVGIPDPSPPFNPFNFNDCSQELCTAFCPQWCA PFPPPPLFGL+DSDDSDTFFSPLI
Sbjct: 1   MDSVGIPDPSPPFNPFNFNDCSQELCTAFCPQWCAFPFPPPPLFGLEDSDDSDTFFSPLI 60

Query: 61  VAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVVNDTRQSSAGPGLNE 120
           VAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVVNDTRQSSAGPGLNE
Sbjct: 61  VAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVVNDTRQSSAGPGLNE 120

Query: 121 ALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSS 180
           ALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSS
Sbjct: 121 ALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSS 180

Query: 181 CPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDST 240
           CPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDST
Sbjct: 181 CPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDST 240

Query: 241 NRDQGSPNPNPNPNPPSVDRGDGRTERNREESIRSIRSVSEDHQSSPCPVRILSVADILS 300
           NRDQGSPNPNP    PSVDRGDGRTER+REESIRSIRSVSEDHQSSPCPVRILSVADILS
Sbjct: 241 NRDQGSPNPNP----PSVDRGDGRTERSREESIRSIRSVSEDHQSSPCPVRILSVADILS 300

Query: 301 INEEEDEEVEAMNSSMAAGTGPSNNDGKETEKSRNRNSVLNSAMRMSIPSGIFSFQRYAK 360
           INEEEDEEVEAMNSSMAAGTGPSNND KETEKSRNRNSVLNSAMRMSIPSGIFSFQRYAK
Sbjct: 301 INEEEDEEVEAMNSSMAAGTGPSNNDDKETEKSRNRNSVLNSAMRMSIPSGIFSFQRYAK 360

Query: 361 GKCSNLHQNFQN 372
           GKCSNLHQ FQN
Sbjct: 361 GKCSNLHQTFQN 368

BLAST of Csor.00g257320 vs. NCBI nr
Match: XP_023006188.1 (RING-H2 finger protein ATL52-like [Cucurbita maxima])

HSP 1 Score: 716 bits (1847), Expect = 1.02e-259
Identity = 359/372 (96.51%), Postives = 365/372 (98.12%), Query Frame = 0

Query: 1   MDSVGIPDPSPPFNPFNFNDCSQELCTAFCPQWCALPFPPPPLFGLDDSDDSDTFFSPLI 60
           MDSVGIPDPSPPFNPFNFNDCSQ+LCTAFCPQWCA PFPPPPLFGLDDSD SDTFFSPLI
Sbjct: 1   MDSVGIPDPSPPFNPFNFNDCSQKLCTAFCPQWCAFPFPPPPLFGLDDSDKSDTFFSPLI 60

Query: 61  VAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVVNDTRQSSAGPGLNE 120
           VAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRV NDTRQSSAGPGLNE
Sbjct: 61  VAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVANDTRQSSAGPGLNE 120

Query: 121 ALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSS 180
           AL+KSISVFKFKKGEGL+EESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSS
Sbjct: 121 ALIKSISVFKFKKGEGLVEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSS 180

Query: 181 CPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDST 240
           CPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDST
Sbjct: 181 CPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDST 240

Query: 241 NRDQGSPNPNPNPNPPSVDRGDGRTERNREESIRSIRSVSEDHQSSPCPVRILSVADILS 300
           NRDQGSPNPNPNP  PSVDRGDGRTE+NREESIRSIRSVSEDHQSSPCPVRILSVADILS
Sbjct: 241 NRDQGSPNPNPNP--PSVDRGDGRTEKNREESIRSIRSVSEDHQSSPCPVRILSVADILS 300

Query: 301 INEEEDEEVEAMNSSMAAGTGPSNNDGKETEKSRNRNSVLNSAMRMSIPSGIFSFQRYAK 360
           I+EEEDEEVEAMNS MAA TGPSNNDGKETEKSRNRNSVLNSAMRMSIPSG+FSFQRYAK
Sbjct: 301 IDEEEDEEVEAMNSPMAADTGPSNNDGKETEKSRNRNSVLNSAMRMSIPSGVFSFQRYAK 360

Query: 361 GKCSNLHQNFQN 372
           GKCSNLHQNFQN
Sbjct: 361 GKCSNLHQNFQN 370

BLAST of Csor.00g257320 vs. NCBI nr
Match: XP_022964349.1 (RING-H2 finger protein ATL52-like [Cucurbita moschata])

HSP 1 Score: 563 bits (1452), Expect = 1.12e-199
Identity = 290/366 (79.23%), Postives = 317/366 (86.61%), Query Frame = 0

Query: 1   MDSVGIPDPSPPFNPFNFNDCSQELCTAFCPQWCALPFPPPP-LFGLDDSDDSDTFFSPL 60
           M+S+GI DPSPPFNPFNF DCSQ++CTAFCPQWCA  FPPPP  FGLDDSDDS TFFSPL
Sbjct: 1   MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVVNDTRQSSAGPGLN 120
           IVAVIGILASAFVLVTYYAIVSKYCRR V DGGG  P E+FE +R +N+TR+SSAG GL+
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120

Query: 121 EALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNS 180
           EAL+KSISV+KFKKGEGLIE SDC++CLSEFQENESLRLLPKCSHAFHL CIDTWLKSNS
Sbjct: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180

Query: 181 SCPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDS 240
           SCP CRSNI  TNPPP ETPPP VT LGYRHSSDAAVVVVQDLS NVSQE VVVEIV+DS
Sbjct: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240

Query: 241 TNRDQGSPNPNPNPNPPSVDRGDGRTERNREESIRSIRSVSEDHQSSPCPVRILSVADIL 300
           TN++Q   NPNP+PNP S D+GDGRTER REES+ SIRSVSEDH SSPCP   LSVAD+L
Sbjct: 241 TNQEQE--NPNPSPNPRSFDQGDGRTER-REESVHSIRSVSEDHHSSPCPGENLSVADVL 300

Query: 301 SINEEEDEEVEAMNSSMAAGTGPSNNDGKETEKSRNRNSVLNSAMRMSIPSGIFSFQRYA 360
           +IN EEDEE+EA++SSMAAG GPSN + KETEKSRN+N VL    RMSI SGIFSF RY 
Sbjct: 301 NINHEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVL----RMSISSGIFSFTRYT 359

Query: 361 KGKCSN 365
           KGK SN
Sbjct: 361 KGKNSN 359

BLAST of Csor.00g257320 vs. ExPASy TrEMBL
Match: A0A6J1H2X8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111459995 PE=3 SV=1)

HSP 1 Score: 738 bits (1906), Expect = 5.39e-269
Identity = 370/372 (99.46%), Postives = 371/372 (99.73%), Query Frame = 0

Query: 1   MDSVGIPDPSPPFNPFNFNDCSQELCTAFCPQWCALPFPPPPLFGLDDSDDSDTFFSPLI 60
           MDSVGIPDPSPPFNPFNFNDCSQELCTAFCPQWCALPFPPPPLFGLDDSDDSDTFFSPLI
Sbjct: 1   MDSVGIPDPSPPFNPFNFNDCSQELCTAFCPQWCALPFPPPPLFGLDDSDDSDTFFSPLI 60

Query: 61  VAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVVNDTRQSSAGPGLNE 120
           VAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVVNDTRQSSAGPGLNE
Sbjct: 61  VAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVVNDTRQSSAGPGLNE 120

Query: 121 ALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSS 180
           ALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSS
Sbjct: 121 ALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSS 180

Query: 181 CPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDST 240
           CPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDST
Sbjct: 181 CPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDST 240

Query: 241 NRDQGSPNPNPNPNPPSVDRGDGRTERNREESIRSIRSVSEDHQSSPCPVRILSVADILS 300
           NRDQGSPNPNPNPNPPSVDRGDGRTERNREESIRSIRSVSEDHQSSPCPVRILSVADILS
Sbjct: 241 NRDQGSPNPNPNPNPPSVDRGDGRTERNREESIRSIRSVSEDHQSSPCPVRILSVADILS 300

Query: 301 INEEEDEEVEAMNSSMAAGTGPSNNDGKETEKSRNRNSVLNSAMRMSIPSGIFSFQRYAK 360
           INEEEDEEVEAMNSSMAAG+GPSNNDGKETEKSRNRNSVLNSAMRMSIPSGIFSFQRY K
Sbjct: 301 INEEEDEEVEAMNSSMAAGSGPSNNDGKETEKSRNRNSVLNSAMRMSIPSGIFSFQRYVK 360

Query: 361 GKCSNLHQNFQN 372
           GKCSNLHQNFQN
Sbjct: 361 GKCSNLHQNFQN 372

BLAST of Csor.00g257320 vs. ExPASy TrEMBL
Match: A0A6J1L482 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111498996 PE=3 SV=1)

HSP 1 Score: 716 bits (1847), Expect = 4.92e-260
Identity = 359/372 (96.51%), Postives = 365/372 (98.12%), Query Frame = 0

Query: 1   MDSVGIPDPSPPFNPFNFNDCSQELCTAFCPQWCALPFPPPPLFGLDDSDDSDTFFSPLI 60
           MDSVGIPDPSPPFNPFNFNDCSQ+LCTAFCPQWCA PFPPPPLFGLDDSD SDTFFSPLI
Sbjct: 1   MDSVGIPDPSPPFNPFNFNDCSQKLCTAFCPQWCAFPFPPPPLFGLDDSDKSDTFFSPLI 60

Query: 61  VAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVVNDTRQSSAGPGLNE 120
           VAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRV NDTRQSSAGPGLNE
Sbjct: 61  VAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVANDTRQSSAGPGLNE 120

Query: 121 ALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSS 180
           AL+KSISVFKFKKGEGL+EESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSS
Sbjct: 121 ALIKSISVFKFKKGEGLVEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSS 180

Query: 181 CPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDST 240
           CPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDST
Sbjct: 181 CPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDST 240

Query: 241 NRDQGSPNPNPNPNPPSVDRGDGRTERNREESIRSIRSVSEDHQSSPCPVRILSVADILS 300
           NRDQGSPNPNPNP  PSVDRGDGRTE+NREESIRSIRSVSEDHQSSPCPVRILSVADILS
Sbjct: 241 NRDQGSPNPNPNP--PSVDRGDGRTEKNREESIRSIRSVSEDHQSSPCPVRILSVADILS 300

Query: 301 INEEEDEEVEAMNSSMAAGTGPSNNDGKETEKSRNRNSVLNSAMRMSIPSGIFSFQRYAK 360
           I+EEEDEEVEAMNS MAA TGPSNNDGKETEKSRNRNSVLNSAMRMSIPSG+FSFQRYAK
Sbjct: 301 IDEEEDEEVEAMNSPMAADTGPSNNDGKETEKSRNRNSVLNSAMRMSIPSGVFSFQRYAK 360

Query: 361 GKCSNLHQNFQN 372
           GKCSNLHQNFQN
Sbjct: 361 GKCSNLHQNFQN 370

BLAST of Csor.00g257320 vs. ExPASy TrEMBL
Match: A0A6J1HMW7 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111464384 PE=3 SV=1)

HSP 1 Score: 563 bits (1452), Expect = 5.42e-200
Identity = 290/366 (79.23%), Postives = 317/366 (86.61%), Query Frame = 0

Query: 1   MDSVGIPDPSPPFNPFNFNDCSQELCTAFCPQWCALPFPPPP-LFGLDDSDDSDTFFSPL 60
           M+S+GI DPSPPFNPFNF DCSQ++CTAFCPQWCA  FPPPP  FGLDDSDDS TFFSPL
Sbjct: 1   MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVVNDTRQSSAGPGLN 120
           IVAVIGILASAFVLVTYYAIVSKYCRR V DGGG  P E+FE +R +N+TR+SSAG GL+
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120

Query: 121 EALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNS 180
           EAL+KSISV+KFKKGEGLIE SDC++CLSEFQENESLRLLPKCSHAFHL CIDTWLKSNS
Sbjct: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180

Query: 181 SCPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDS 240
           SCP CRSNI  TNPPP ETPPP VT LGYRHSSDAAVVVVQDLS NVSQE VVVEIV+DS
Sbjct: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240

Query: 241 TNRDQGSPNPNPNPNPPSVDRGDGRTERNREESIRSIRSVSEDHQSSPCPVRILSVADIL 300
           TN++Q   NPNP+PNP S D+GDGRTER REES+ SIRSVSEDH SSPCP   LSVAD+L
Sbjct: 241 TNQEQE--NPNPSPNPRSFDQGDGRTER-REESVHSIRSVSEDHHSSPCPGENLSVADVL 300

Query: 301 SINEEEDEEVEAMNSSMAAGTGPSNNDGKETEKSRNRNSVLNSAMRMSIPSGIFSFQRYA 360
           +IN EEDEE+EA++SSMAAG GPSN + KETEKSRN+N VL    RMSI SGIFSF RY 
Sbjct: 301 NINHEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVL----RMSISSGIFSFTRYT 359

Query: 361 KGKCSN 365
           KGK SN
Sbjct: 361 KGKNSN 359

BLAST of Csor.00g257320 vs. ExPASy TrEMBL
Match: A0A6J1KJE3 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111494536 PE=3 SV=1)

HSP 1 Score: 557 bits (1435), Expect = 1.81e-197
Identity = 289/366 (78.96%), Postives = 312/366 (85.25%), Query Frame = 0

Query: 1   MDSVGIPDPSPPFNPFNFNDCSQELCTAFCPQWCALPFPPPP-LFGLDDSDDSDTFFSPL 60
           MDS+GI DPSPPFNPFNF DCSQ++CTAFCPQWCA  FPPPP  FGLDDSDDS TFFSPL
Sbjct: 1   MDSIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVVNDTRQSSAGPGLN 120
           IVAVIGILASAFVLVTYYAIVSKYCRR V DGGG  P E+FE +R +N+TR+SSAG GL+
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVSDGGGGGPEESFETNRAINETRRSSAGSGLD 120

Query: 121 EALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNS 180
           EAL+KSISV+KFKKGEGLIE SDC++CLSEFQENESLRLLPKCSHAFHL CIDTWLKSNS
Sbjct: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180

Query: 181 SCPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDS 240
           SCP CRSNI  TNPPP ETPPP VT LGYRHSSDAAVVVVQDLS NVSQE VVVEIV+DS
Sbjct: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240

Query: 241 TNRDQGSPNPNPNPNPPSVDRGDGRTERNREESIRSIRSVSEDHQSSPCPVRILSVADIL 300
           TN++Q       NPNP S D+GDGRTER REESI SIRSVSEDH SSPCP   LSVAD+L
Sbjct: 241 TNQEQE------NPNPRSFDQGDGRTER-REESIHSIRSVSEDHHSSPCPGENLSVADVL 300

Query: 301 SINEEEDEEVEAMNSSMAAGTGPSNNDGKETEKSRNRNSVLNSAMRMSIPSGIFSFQRYA 360
           SIN EEDEE+EA++SSMAAG GPSN + KETEKSRN+N VL    RMSI SGIFSF RY 
Sbjct: 301 SINHEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVL----RMSISSGIFSFTRYT 355

Query: 361 KGKCSN 365
           KGK  N
Sbjct: 361 KGKNPN 355

BLAST of Csor.00g257320 vs. ExPASy TrEMBL
Match: A0A6J1DSH1 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111022765 PE=3 SV=1)

HSP 1 Score: 540 bits (1391), Expect = 1.61e-190
Identity = 292/377 (77.45%), Postives = 314/377 (83.29%), Query Frame = 0

Query: 1   MDSVGIPDPSPPFNPFNFNDCSQELCTAFCPQWCALPFPPPP-LFGLDDSDDSDTFFSPL 60
           MDSVGIPDPSPPFNPFN  DCSQ +CTAFCPQWC   FPPPP  FGLDDSDDS TFFSPL
Sbjct: 1   MDSVGIPDPSPPFNPFNLKDCSQGICTAFCPQWCRYVFPPPPPFFGLDDSDDSGTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAE---PVENFEADRVVNDTRQ-SSAG 120
           IVAVIGILASAFVLVTYYAIVSKYCRRRVD GGG     P + FE DR +ND R+ S AG
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRRVDSGGGGAGGGPGDYFETDRAMNDARRPSGAG 120

Query: 121 PGLNEALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWL 180
           PGL+EAL+KSISVFKFKK EGLIE SDC++CLSEFQENESLRLLPKCSHAFHL CIDTWL
Sbjct: 121 PGLDEALIKSISVFKFKKNEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLVCIDTWL 180

Query: 181 KSNSSCPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEI 240
           KSNSSCP CR NI  TNP PPETPPPSVT LGYRH SDAAVVVVQD SENVSQE VVVEI
Sbjct: 181 KSNSSCPLCRCNIASTNPNPPETPPPSVTALGYRHRSDAAVVVVQDPSENVSQEAVVVEI 240

Query: 241 VTDS----TNRDQGSPNPNPNPNPPSVDRGDGRTERNREESIRSIRSVSEDHQSS--PCP 300
           +  S    +N+DQG  N NP    PS ++GDG TER REE++RSIRSVSED QSS  PC 
Sbjct: 241 IGHSISNPSNQDQGGSNSNP----PSSEQGDGGTER-REETVRSIRSVSEDQQSSDSPCL 300

Query: 301 VRILSVADILSINEEEDEEVEAMNSSMAAGTGPSNNDGKETEKSRNRNSVLNSAMRMSIP 360
            R LSVADILSI+ +EDEEVEA++SSMAAG GPSN+DGKET KSR RN VLNSAMRMSIP
Sbjct: 301 GRNLSVADILSISHDEDEEVEAVDSSMAAGIGPSNDDGKETGKSRIRNGVLNSAMRMSIP 360

Query: 361 SGIFSFQRYAKGKCSNL 366
           SGIFSF ++AKGK SNL
Sbjct: 361 SGIFSFAKHAKGKSSNL 372

BLAST of Csor.00g257320 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 222.6 bits (566), Expect = 5.2e-58
Identity = 144/372 (38.71%), Postives = 210/372 (56.45%), Query Frame = 0

Query: 7   PDPSPPFNPFNFNDCSQELCTAFCPQWCALPFPPPP--LFGLDDSDDSDTFFSPLIVAVI 66
           P+P  P++  ++NDCSQ +C  +CPQWC L FPPPP   F  DDS  S + FSPL++A+I
Sbjct: 5   PNPWSPYD--SYNDCSQGICNIYCPQWCYLIFPPPPPSFFLDDDSSSSSSSFSPLLIALI 64

Query: 67  GILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVVNDT-RQSSAGPGLNEALL 126
           GIL SA +LV+YY ++SKYC R            N   +   + T R S+ G GLNE+++
Sbjct: 65  GILTSALILVSYYTLISKYCHRHHQTSSSETLNLNHNGEGFFSSTQRISTNGDGLNESMI 124

Query: 127 KSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSSCPF 186
           KSI+V+K+K G+G ++ SDC++CLSEF+ENESLRLLPKC+HAFHL CIDTWLKS+S+CP 
Sbjct: 125 KSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHSNCPL 184

Query: 187 CRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVS----------QEVVVV 246
           CR+ +T  N P          V+  + +S      V +++ N++            VV+ 
Sbjct: 185 CRAFVTGVNNPTASVGQNVSVVVANQSNSAHQTGSVSEINLNLAGYESQTGDFDSVVVIE 244

Query: 247 EIVTDSTNRDQGSPNPNPNPNPPSVDRGDGRTERNREESIRSIRSVSEDHQSSPCPVRIL 306
           ++   S N D  S    P          + R E   E+S+   RSVS +         ++
Sbjct: 245 DLEIGSRNSDARSELQLP----------EERRETKDEDSLPIRRSVSLNSGV------VV 304

Query: 307 SVADILSINEEEDEEVEAMNSSMAAGTGPSNNDGK---ETEKSRNRNSVLNSAMRMSIPS 363
           S+AD+L   E+E+ E   + +S     G  + DGK    TE ++    V    +R S+ +
Sbjct: 305 SIADVLREIEDEEGESGGVGTSQRREEG-EDGDGKTIPPTEANQRSGGVSGFFVR-SLST 356

BLAST of Csor.00g257320 vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 211.1 bits (536), Expect = 1.6e-54
Identity = 139/367 (37.87%), Postives = 200/367 (54.50%), Query Frame = 0

Query: 1   MDSVGIPDPSPPFNPFNFNDCSQELCTAFCPQWCALPFPPPPLFGLDDSDDSDTF-FSPL 60
           M S G P+P       ++ DCSQ +C+ +CPQWC + FPPPP F LDD DDS +  FSPL
Sbjct: 1   MGSTGNPNPWGTTYD-SYRDCSQGVCSVYCPQWCYVIFPPPPSFYLDDEDDSSSSDFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRRVDDGG--GAEPVENFEADRV------------ 120
           ++A+IGILASAF+LV+YY ++SKYC RR  +     A  +    +D              
Sbjct: 61  LIALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNNNNNGA 120

Query: 121 --VNDTRQSSAGPGLNEALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCS 180
              N T     G GL+E+L+KSI+V+K++K +G +E SDC++CLSEFQENESLRLLPKC+
Sbjct: 121 TNPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCN 180

Query: 181 HAFHLACIDTWLKSNSSCPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLS 240
           HAFH+ CIDTWLKS+S+CP CR+ I  ++         +V ++     ++  +V   +  
Sbjct: 181 HAFHVPCIDTWLKSHSNCPLCRAFIVTSS---------AVEIVDL---TNQQIVTENNSI 240

Query: 241 ENVSQEVVVVEIVTDSTNRDQGSPNPNPNPNPPSV-DRGDGRTERNREESIRSIRSVSED 300
                 VVVV +  +++     + N    P PP + D  DG  E  R  S+ S   V   
Sbjct: 241 STGDDSVVVVNLDLENSRSRNETVNEGSTPKPPEMQDSRDG--EERRSASLNSGGGV--- 300

Query: 301 HQSSPCPVRILSVADILSINEEEDEEVEAMNSSMAAGTGPSNNDGKETEKSRNR-NSVLN 349
                     +S+ADIL    E+DEE   + +S     G        +  + N+ N + N
Sbjct: 301 ----------VSIADILR-EIEDDEESAGVGTSRWVEEGEGEKTPPPSGSAANQTNGISN 338

BLAST of Csor.00g257320 vs. TAIR 10
Match: AT4G17905.1 (RING/U-box superfamily protein )

HSP 1 Score: 160.2 bits (404), Expect = 3.1e-39
Identity = 99/243 (40.74%), Postives = 134/243 (55.14%), Query Frame = 0

Query: 7   PDPSPPFNPFNFNDCSQELCTAFCPQWCA-LPFPPPPLF---GLDDSDDSDTFFSPLIVA 66
           P+PS      N  DC+Q LC+ FCPQWC  + F PPP+     L+D   S+   SPL++A
Sbjct: 5   PNPSAWNQYINPRDCTQGLCSTFCPQWCTYINFSPPPISYEQFLNDGVASNPNLSPLVIA 64

Query: 67  VIGILASAFVLVTYYAIVSKYCRRRVDDGGGAE--------PVENFEADRVVNDTRQSSA 126
           + GI A+AF+L  YY +VSKYC     +   +E         V + E     +     S+
Sbjct: 65  IFGIFATAFLLAAYYTLVSKYCANDTTNEAASESGRSDIILDVNSPERGDQDDPFALESS 124

Query: 127 GPGLNEALLKSISVFKFKKGEG--LIEESDCAICLSEFQENESLRLLPKCSHAFHLACID 186
             GL++ L+K I  FK KK +    I  +DC+ICL EF E+ESLRLLPKC+H FH+ CID
Sbjct: 125 TAGLDDTLIKKIGFFKLKKHQNGFKINGTDCSICLGEFNEDESLRLLPKCNHTFHVVCID 184

Query: 187 TWLKSNSSCPFCRSNITPTNPPPPETPPPSVTVLGY-RHSSDAAV----VVVQDLSENVS 231
            WLKS+S+CP CR+ I     P  + P   V V+   R +S+       VVV D  E VS
Sbjct: 185 RWLKSHSNCPLCRAKII---VPTTQQPEHHVVVMNLDRFTSNVGSAEGNVVVDDHREEVS 244

BLAST of Csor.00g257320 vs. TAIR 10
Match: AT5G43420.1 (RING/U-box superfamily protein )

HSP 1 Score: 122.5 bits (306), Expect = 7.3e-28
Identity = 77/188 (40.96%), Postives = 110/188 (58.51%), Query Frame = 0

Query: 37  PFPPPPLFGLDDSDDSDTFFSPLIVAVIGILASAFVLVTYYAIVSKYCR--RRVDDGGG- 96
           P PPPP+F    +  + T F  L VAVIGILA+AF+LV+YY  V K C    R+D  G  
Sbjct: 17  PPPPPPIF--HRASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRIDILGRF 76

Query: 97  --AEPVENFEADRVVNDTRQSSAGPGLNEALLKSISVFKFKK----------GEGLIEE- 156
             +    N +   +V      S   GL+E+++++I +FKFKK          GEG  EE 
Sbjct: 77  SLSRRRRNDQDPLMVYSPELRSR--GLDESVIRAIPIFKFKKRYDQNDGVFTGEGEEEEE 136

Query: 157 ---SDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSSCPFCRSNIT-PTNPPPP 205
               +C++CLSEFQ+ E LR++P CSH FH+ CID WL++N++CP CR+ ++  T+ PP 
Sbjct: 137 KRSQECSVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRVSCDTSFPPD 196

BLAST of Csor.00g257320 vs. TAIR 10
Match: AT1G72220.1 (RING/U-box superfamily protein )

HSP 1 Score: 118.2 bits (295), Expect = 1.4e-26
Identity = 75/224 (33.48%), Postives = 120/224 (53.57%), Query Frame = 0

Query: 3   SVGIPDPSPPFNPF---NFNDCSQELCTAFCPQWCALPFPPP------PLFGLDDSDDSD 62
           S G+ DP  P+N +   ++   S +L     P W + P P P          L   D S 
Sbjct: 24  SNGVCDPICPYNCYPEPDYYTISPQL-----PPWSSSPQPSPCPSPSISAVYLPSQDSSS 83

Query: 63  TFFSPLIVAVIGILASAFVLVTYYAIVSKYCRRRVDD-GGGAEPVENFEADRVVNDTRQS 122
           +  +  I+ + G + +  +L+T + +V+K+    V+    G    +N + D V+ +  Q 
Sbjct: 84  SLDAISIITITGAVLA--ILLTGFFLVAKFFSDSVNRVNQGTYQSDNEDNDTVMEEEFQD 143

Query: 123 S----------AGPGLNEALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKC 182
                         GL ++++ SI++  +K+G+GLIE +DC +CL+EF+E+ESLRLLPKC
Sbjct: 144 REQVDHPIWLIRTTGLQQSIINSITICNYKRGDGLIERTDCPVCLNEFEEDESLRLLPKC 203

Query: 183 SHAFHLACIDTWLKSNSSCPFCRSNI------TPTNPPPPETPP 201
           +HAFH++CIDTWL S+++CP CR+ I      TP    P E  P
Sbjct: 204 NHAFHISCIDTWLSSHTNCPLCRAGIAMISVTTPRYSGPVEVTP 240

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LF647.3e-5738.71RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1[more]
Q9SRQ82.2e-5337.87RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2[more]
Q7XLY82.9e-4537.32E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=3994... [more]
P0C0414.4e-3840.74Putative RING-H2 finger protein ATL53 OS=Arabidopsis thaliana OX=3702 GN=ATL53 P... [more]
Q9LSW91.0e-2640.96RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6574830.16.72e-270100.00RING-H2 finger protein ATL52, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022958842.11.11e-26899.46RING-H2 finger protein ATL52-like [Cucurbita moschata][more]
XP_023547750.12.32e-26097.58RING-H2 finger protein ATL52-like [Cucurbita pepo subsp. pepo][more]
XP_023006188.11.02e-25996.51RING-H2 finger protein ATL52-like [Cucurbita maxima][more]
XP_022964349.11.12e-19979.23RING-H2 finger protein ATL52-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1H2X85.39e-26999.46RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111459995... [more]
A0A6J1L4824.92e-26096.51RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111498996 P... [more]
A0A6J1HMW75.42e-20079.23RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111464384... [more]
A0A6J1KJE31.81e-19778.96RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111494536 P... [more]
A0A6J1DSH11.61e-19077.45RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102276... [more]
Match NameE-valueIdentityDescription
AT5G17600.15.2e-5838.71RING/U-box superfamily protein [more]
AT3G03550.11.6e-5437.87RING/U-box superfamily protein [more]
AT4G17905.13.1e-3940.74RING/U-box superfamily protein [more]
AT5G43420.17.3e-2840.96RING/U-box superfamily protein [more]
AT1G72220.11.4e-2633.48RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 143..184
e-value: 2.0E-8
score: 44.0
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 142..185
e-value: 3.4E-14
score: 52.9
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 143..185
score: 13.123657
NoneNo IPR availableSMARTSM01197FANCL_C_2coord: 139..192
e-value: 0.0014
score: 5.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 305..339
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 238..288
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 260..278
NoneNo IPR availablePANTHERPTHR45676:SF108E3 UBIQUITIN-PROTEIN LIGASE OS04G0590900-LIKEcoord: 17..204
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 142..185
e-value: 1.26726E-17
score: 73.8753
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 137..193
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 118..192
e-value: 1.2E-22
score: 80.9
IPR044675E3 ubiquitin-protein ligase RING1-likePANTHERPTHR45676RING-H2 FINGER PROTEIN ATL51-RELATEDcoord: 17..204

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g257320.m01Csor.00g257320.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane