Csor.00g256740 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g256740
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionPeroxisomal membrane protein PEX16
LocationCsor_Chr17: 35924 .. 39417 (-)
RNA-Seq ExpressionCsor.00g256740
SyntenyCsor.00g256740
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGCTTACAAGAAGTGGGTTAGGGATAACAGAGACTACCTTCACTCTTTTGAGTCTCTTGCAAATGTGAGCACTATAGATTTCTTATCATTACCCGCTGGTTTCTCTATTTGATTTCATCTGGGAATTTATTTGGTTGACTTGTTTGTTCTTTAATCTGTACGTAAACGGTGCTGCCCTTGGCTTTTATGGCGTTCTCTATTAAGTAATATTAGCATCTTTTTGCTAGTAGTTTATTTAAAGAACGGCTTTATGTTTCCACAGTTTATTTTCTGCAAATTTAAGATGGCATGCATGTCATGATGTTGATTTGATTTTTAAAAGAATAAGAGATATAGACAACAGATGGTATCCATATGTCTGATAGCAATGAGCATGAAGAGACCATTAAGTCAATTTATTCCTTGAAAATGAAGATTGATATACTATGGTCATTTTTTGTGAGTTTTGAATGTTCAACACTAGTCGATCGCCCGGCATTTTTATTGAAATCAGTTTGGGTAAACTGCACCTAAGTGCGTTTAATGTTATTAACATGTTCATTCTACATGATTATGTTCCTGTATGAGAGTACCCGCAAACTTTCTTATATACCATGAAACATATCGTCATTTGGTTATATGGATCTTATTATATTTTGATGTTTTTGATGGTCTGTGTATTGTTTAGGTATTGAAATTGTAACAGCCTAAGCCTATCGCTAGCCGATATTGTCTTTTTTGGACTTTCCCTTTTGGGTTTCCCTTCAAGGTTTTAAAACGTGTCTAGTAGGGATAGATTTCCACACTCTTGTAAAGAATGCTTCGTTCTCCTCTTCAACCAATGTGAGATCCCACTATCCACCCTCCTTTGGGGCCCAACATCCTTGTTGGCACTCGTTCGGATTGTGAGATCCCACGTCGGTTGGAGAGGGGAACAAAACATTCTTTATAAGGGTGTAAAAACTTCTCCCTAACATATGTGTTTTAAAACCTTGAGGGGAAACCCCGAAGGGAAAACCCAAAAAGGACAACATCTGTTATCGGTGGGCTTGGGCTGTTATAGAAATATAGAAAATACCGAACTATTTGCATGTCGGTGAATGAGCCTTTTTCACTGTTTGATCACAAGCAATTGTAACCCCCCTTGTAATAGATCTTTTACTGCTTGCTTGTGTTGGCCTCATTATAATGCGGAACAACCCATTCAAATTACCTTGCAATGAGAGAAATAGAGTAGATTGGCTGAAAAGTTGAACCACCTTACTTTGACAGACATGTTTAATCGATATTATTAGTACATAATCTCCTCTAGAGGTTAATCTTACGGCAATTGTAGAACAATTTTTTTGCCATTTGAAAGTAGAACTGGGATGGTGGAATCTTGTTCAAGGATCATGGAAATATCAACGTTGGATGGGTTTCCATGTGCTAATTTGGTGCATGATGTATTTTGTCATCTCATGTTTGTTTGTCATAAAACAGGTTAATTTCTCGATAGTTTTTGAGAACTGTTATATTACCATGATTAAAGTCAACTGTTAGTAGATCTGAGAGTATAAGTTTGTGGATTTCTACCTTTTATATGGTTCTTAATACTGTACAGGGCTTGACGTGGCTTCTTCCTGAACGATTTTCTGATTCAGAAATTGGACCTGAAGCAGGTTTTGTGAAACTTTGGTAACACTTGACTGCTTCTTCTGAGCTTGGAATTTGTAGTGCCCTTGGGTCCCCTTTGATGAATATGCATGTCTTCATATTTGTCTCACTTTCAAATGCTATAAATTTACAGTAGACCATATCAAAAGCATTCTGATAGCCAGCAAGGTTTTCTTAAGTTATTCTGTTTTCATTTTTCAACTTAGGGGTTCGAACCTCGTAACTTTGGAAGCTATCTCCCTACGTTCTAAGTTTGTACCAACAAACGTGAAAATTTAACTGCTATTTTGCTGTTTACTGGCGTTGTCTTGTCAAATGAACACGTTCCTGGCTTCTTTGTTCTAAAGTTTTGCCTTGCTCTGAGTGGATGATTTGCAACTTTGAAGCTATTATTAATTAGTTTAGGGATTTCTGCAGTTTCTGCCGCTGTTGGCATTGTCACTGCAGTCAATGAACACATAATCGAAACAGCTCCAACCCAGATGCATGTAAACGCAACTGTGCCATCTTCATTTCCTTATTCACTATGCATATCTGCTCTGAAAAACTTGGAAACATTGATTGAAGTTGCTGCACAACAATATTATGGTGATGATAAACGATGGAACTTCATTGCTGTTACGGAGGCTTTGAAGTAAGACACAGTTGATGAGTGTGGGAGTGCCATTTTTTAATCTTCTGATACATTTTTATTGCATAGTCTTTGCCTTTACATTAGTAGCCGTTGAATTTCAGGGTACTCTTTAGATTAGCTCTGTTCAAGAATTGTGGGTACAAGATCGTTCTTGATGGAGGAGAAACCATAAATAACGAAAGACATCTGAAAACTTCAACTTCTCAGCACAGACCTAATGCTTTCACAAAGCATGGTGGGCATAATGCATCTGGTTATTCAGGATATCTTAATGGACATGACCAATGGAATCTAGAAGGGAGGGCATTATCTGCATTAAGCAGGTTCGGGGAAAAGGCTCGGATGATTTCACCACCAGCATGGTCGTATGGGGTTCAGCACCAGCATGGAATGTTCAATCCTTCAGGTGCCTATATCGATCCCAAGTTCCTAGAGTTTTGTGGGGGGAATGTCTACTGACAATTAATTGTGCGTTTACAGCTACAACGCCTGAGAGGCCTACACTTTCTACTATTTTGTCTGAACAGGGTCATCGCGGGGCTCTTTTCGTGACTGGAGAAGTACTTTTCATAACACGACCGCTTATTTACGTTCTTCTAATCCGCAAATATGGGAGTCGGTCGTGGACTCCTTGGTTTCTTTCACTGGCTGTGGACCTCCTTGGGACAAGCTTTTTATCATATGCTACCTCAGCATCGACGAGTAGAAAAAACCAAAGATCATGCCTTTCCGACTCTGAGAAGGATGAGGTCGTGTACTCTAGTTCATCCTACATAGTAACTTTATTGGGTTAAAAACTTAACTGTCATTCTCATATTAACTTCAACATGTTTGACTTGGTTCAGCTGAGAAGACGGAAGATGCTTTGGGCATTTTACCTCATGAGAGACCCATTCTTCGAGAGATATACAAGGTGCATACATGCTTCAAGATTTAAACTTTTTGAGTCGAGAATTTCTCGACTTCAAACTTATAAGTCTTGGATTGAAACCGATTATGCTGAATGTTTTTTCAGGCGAAAACTTGAAGGGGCCGAAAAAGTTTTAGAACCTGTACCTTTTGTCGGATTCCTCACAGGTTCGAAGTGTATCCATTTGCGATAGTTGTAGATTTAAGATGATGTGGATTATAGCTGATATTTACTGTGTTGATGTGCAGCAAAAATTGTTGAGCTTATTATTGGAGCTCAGACAAGATATACTTACATGTCAGCGTCATGA

mRNA sequence

ATGGAAGCTTACAAGAAGTGGGTTAGGGATAACAGAGACTACCTTCACTCTTTTGAGTCTCTTGCAAATGGCTTGACGTGGCTTCTTCCTGAACGATTTTCTGATTCAGAAATTGGACCTGAAGCAGTTTCTGCCGCTGTTGGCATTGTCACTGCAGTCAATGAACACATAATCGAAACAGCTCCAACCCAGATGCATGTAAACGCAACTGTGCCATCTTCATTTCCTTATTCACTATGCATATCTGCTCTGAAAAACTTGGAAACATTGATTGAAGTTGCTGCACAACAATATTATGGTGATGATAAACGATGGAACTTCATTGCTGTTACGGAGGCTTTGAAGGTACTCTTTAGATTAGCTCTGTTCAAGAATTGTGGGTACAAGATCGTTCTTGATGGAGGAGAAACCATAAATAACGAAAGACATCTGAAAACTTCAACTTCTCAGCACAGACCTAATGCTTTCACAAAGCATGGTGGGCATAATGCATCTGGTTATTCAGGATATCTTAATGGACATGACCAATGGAATCTAGAAGGGAGGGCATTATCTGCATTAAGCAGGTTCGGGGAAAAGGCTCGGATGATTTCACCACCAGCATGGTCGTATGGGGTTCAGCACCAGCATGGAATGTTCAATCCTTCAGCTACAACGCCTGAGAGGCCTACACTTTCTACTATTTTGTCTGAACAGGGTCATCGCGGGGCTCTTTTCGTGACTGGAGAAGTACTTTTCATAACACGACCGCTTATTTACGTTCTTCTAATCCGCAAATATGGGAGTCGGTCGTGGACTCCTTGGTTTCTTTCACTGGCTGTGGACCTCCTTGGGACAAGCTTTTTATCATATGCTACCTCAGCATCGACGAGTAGAAAAAACCAAAGATCATGCCTTTCCGACTCTGAGAAGGATGAGCTGAGAAGACGGAAGATGCTTTGGGCATTTTACCTCATGAGAGACCCATTCTTCGAGAGATATACAAGGCGAAAACTTGAAGGGGCCGAAAAAGTTTTAGAACCTGTACCTTTTGTCGGATTCCTCACAGCAAAAATTGTTGAGCTTATTATTGGAGCTCAGACAAGATATACTTACATGTCAGCGTCATGA

Coding sequence (CDS)

ATGGAAGCTTACAAGAAGTGGGTTAGGGATAACAGAGACTACCTTCACTCTTTTGAGTCTCTTGCAAATGGCTTGACGTGGCTTCTTCCTGAACGATTTTCTGATTCAGAAATTGGACCTGAAGCAGTTTCTGCCGCTGTTGGCATTGTCACTGCAGTCAATGAACACATAATCGAAACAGCTCCAACCCAGATGCATGTAAACGCAACTGTGCCATCTTCATTTCCTTATTCACTATGCATATCTGCTCTGAAAAACTTGGAAACATTGATTGAAGTTGCTGCACAACAATATTATGGTGATGATAAACGATGGAACTTCATTGCTGTTACGGAGGCTTTGAAGGTACTCTTTAGATTAGCTCTGTTCAAGAATTGTGGGTACAAGATCGTTCTTGATGGAGGAGAAACCATAAATAACGAAAGACATCTGAAAACTTCAACTTCTCAGCACAGACCTAATGCTTTCACAAAGCATGGTGGGCATAATGCATCTGGTTATTCAGGATATCTTAATGGACATGACCAATGGAATCTAGAAGGGAGGGCATTATCTGCATTAAGCAGGTTCGGGGAAAAGGCTCGGATGATTTCACCACCAGCATGGTCGTATGGGGTTCAGCACCAGCATGGAATGTTCAATCCTTCAGCTACAACGCCTGAGAGGCCTACACTTTCTACTATTTTGTCTGAACAGGGTCATCGCGGGGCTCTTTTCGTGACTGGAGAAGTACTTTTCATAACACGACCGCTTATTTACGTTCTTCTAATCCGCAAATATGGGAGTCGGTCGTGGACTCCTTGGTTTCTTTCACTGGCTGTGGACCTCCTTGGGACAAGCTTTTTATCATATGCTACCTCAGCATCGACGAGTAGAAAAAACCAAAGATCATGCCTTTCCGACTCTGAGAAGGATGAGCTGAGAAGACGGAAGATGCTTTGGGCATTTTACCTCATGAGAGACCCATTCTTCGAGAGATATACAAGGCGAAAACTTGAAGGGGCCGAAAAAGTTTTAGAACCTGTACCTTTTGTCGGATTCCTCACAGCAAAAATTGTTGAGCTTATTATTGGAGCTCAGACAAGATATACTTACATGTCAGCGTCATGA

Protein sequence

MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIETAPTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRLALFKNCGYKIVLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLEGRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHRGALFVTGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSASTSRKNQRSCLSDSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQTRYTYMSAS
Homology
BLAST of Csor.00g256740 vs. ExPASy Swiss-Prot
Match: Q8S8S1 (Peroxisome biogenesis protein 16 OS=Arabidopsis thaliana OX=3702 GN=PEX16 PE=1 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 1.2e-107
Identity = 210/374 (56.15%), Postives = 265/374 (70.86%), Query Frame = 0

Query: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60
           MEAYK+WV  NR+Y+ SF S ANGLTWLLPE+FS SEIGPEAV+A +GI + +NEHIIE 
Sbjct: 1   MEAYKQWVWRNREYVQSFGSFANGLTWLLPEKFSASEIGPEAVTAFLGIFSTINEHIIEN 60

Query: 61  APT-QMHVNAT-VPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLF 120
           APT + HV ++    S  Y L I+ LK+LET++EVAA+ +YG DK+WN+I +TEA+K + 
Sbjct: 61  APTPRGHVGSSGNDPSLSYPLLIAILKDLETVVEVAAEHFYG-DKKWNYIILTEAMKAVI 120

Query: 121 RLALFKNCGYKIVLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYS-GYLNGHDQW 180
           RLALF+N GYK++L GGET N E+    S SQ+R      + G N   +  G  N H+ W
Sbjct: 121 RLALFRNSGYKMLLQGGETPNEEKDSNQSESQNRAG----NSGRNLGPHGLGNQNHHNPW 180

Query: 181 NLEGRALSALSRFGEKARMI--SPPAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHR 240
           NLEGRA+SALS FG+ AR    S P WS  +QHQ  +  P      R T+S +L+E+G  
Sbjct: 181 NLEGRAMSALSSFGQNARTTTSSTPGWSRRIQHQQAVIEPPMIKERRRTMSELLTEKGVN 240

Query: 241 GALFVTGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSASTSRKNQ 300
           GALF  GEVL+ITRPLIYVL IRKYG RSW PW +SL+VD LG   L  A S     K++
Sbjct: 241 GALFAIGEVLYITRPLIYVLFIRKYGVRSWIPWAISLSVDTLGMGLL--ANSKWWGEKSK 300

Query: 301 RSCLSDSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVEL 360
           +   S  EKDELRRRK++WA YLMRDPFF +YTR+KLE ++K LE +P +GFLT KIVEL
Sbjct: 301 QVHFSGPEKDELRRRKLIWALYLMRDPFFTKYTRQKLESSQKKLELIPLIGFLTEKIVEL 360

Query: 361 IIGAQTRYTYMSAS 370
           + GAQ+RYTY+S S
Sbjct: 361 LEGAQSRYTYISGS 367

BLAST of Csor.00g256740 vs. ExPASy Swiss-Prot
Match: Q91XC9 (Peroxisomal membrane protein PEX16 OS=Mus musculus OX=10090 GN=Pex16 PE=1 SV=2)

HSP 1 Score: 97.1 bits (240), Expect = 4.6e-19
Identity = 92/371 (24.80%), Postives = 159/371 (42.86%), Query Frame = 0

Query: 4   YKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIETAPT 63
           Y+++V  +       E+   GL++LL  RFSDS    E V +A  ++  +N+ I+     
Sbjct: 11  YQEYVTRHPAATAQLETAVRGLSYLLAGRFSDSHELSELVYSASNLLVLLNDGIL----- 70

Query: 64  QMHVNATVPSSFPYS---LCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 123
           +  +   +P S         +S L+ +E  +E+ A + +G+  RW  IA+ +  K + R+
Sbjct: 71  RKELRKKLPVSLSQQKLLTWLSVLECVEVFMEMGAAKVWGEVGRWLVIALIQLAKAVLRM 130

Query: 124 ALFKNCGYKIVLDGGETI---NNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQW 183
            L     +K  +     I   + E   +     H P                   G  + 
Sbjct: 131 LLL--IWFKAGIQTSPPIVPLDRETQAQPLDGDHNP-------------------GSQEP 190

Query: 184 NLEGRALSALSRFGEKARMISPPAWSYGVQHQ--HGMFNPSATTPERPTLSTILSEQGHR 243
           +  G+    + R  + +  +    W    Q +          +TP  P            
Sbjct: 191 SYVGKRSHRVVRTLQNSPSLHSRYWGAPQQREIRQKQQQEELSTPPTPL----------- 250

Query: 244 GALFVTGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSASTSRKNQ 303
           G      E L+I RPL+++L +  +G RSWTPW LS  VD+   S LS        RKN 
Sbjct: 251 GLQETIAESLYIARPLLHLLSLGLWGQRSWTPWLLSGVVDMTSLSLLS-------DRKN- 310

Query: 304 RSCLSDSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVL-EPVPFVGFLTAKIVE 363
              L+  E+ ELRRR +L  +YL+R PF++R++  K+    ++L + +P VG +   +++
Sbjct: 311 ---LTRRERLELRRRTILLLYYLLRSPFYDRFSEAKILFLLQLLTDHIPGVGLVARPLMD 333

Query: 364 LIIGAQTRYTY 366
            +   Q  Y Y
Sbjct: 371 YLPSWQKIYFY 333

BLAST of Csor.00g256740 vs. ExPASy Swiss-Prot
Match: B0JYZ2 (Peroxisomal membrane protein PEX16 OS=Xenopus tropicalis OX=8364 GN=pex16 PE=2 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 7.8e-19
Identity = 93/365 (25.48%), Postives = 152/365 (41.64%), Query Frame = 0

Query: 2   EAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIETA 61
           + YK +V  N       ES    L++L+  RF+DS    E V +A  ++  +N+ I+   
Sbjct: 14  QKYKDYVIQNPTGATQLESAVRMLSYLIAGRFADSHELSELVYSASNLLALLNDGILR-- 73

Query: 62  PTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRLA 121
              +    T  S       +  L++LE  IE+ A + +GD  RW  I + + LK   R+ 
Sbjct: 74  KELLAPPPTEGSRRRLLTWLGVLESLEVFIEIGAARAWGDRTRWAAILIIQLLKACLRIV 133

Query: 122 LFKNCGYKIVLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLEG 181
           L     Y+  +     +         T   R     +   ++ SG S ++          
Sbjct: 134 LL--FWYRAGIQSSPPV---------TPLDREGILNQAEDNSNSGSSCFVG-----RRSS 193

Query: 182 RALSALSRFGEKARMISPPAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHRGALFVT 241
           RA+ +L       R      W     H     N   T  ++       SE G  G L   
Sbjct: 194 RAVRSLDDSASSHRRF----WRSPQIHDGKQRNTGETESDKDG-----SELGTLGTL--- 253

Query: 242 GEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSASTSRKNQRSCLSD 301
            E + I RP+ ++L +  +G +SW PW ++ A+D+   S LS   +           LS 
Sbjct: 254 AEAIHILRPITHLLSLATWGQKSWKPWMVAAALDITSISLLSDVRN-----------LSH 313

Query: 302 SEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVL-EPVPFVGFLTAKIVELIIGAQ 361
            E+ ELRRR  L  +YL+R PF+  YT  +L    ++L + VP VG +   +++ +   Q
Sbjct: 314 RERAELRRRMFLLLYYLLRSPFYNHYTETRLLLLLRLLGDYVPGVGLVARPLMDYLPVWQ 337

Query: 362 TRYTY 366
             Y Y
Sbjct: 374 KIYFY 337

BLAST of Csor.00g256740 vs. ExPASy Swiss-Prot
Match: Q9Y5Y5 (Peroxisomal membrane protein PEX16 OS=Homo sapiens OX=9606 GN=PEX16 PE=1 SV=2)

HSP 1 Score: 95.1 bits (235), Expect = 1.7e-18
Identity = 92/369 (24.93%), Postives = 157/369 (42.55%), Query Frame = 0

Query: 4   YKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIETAPT 63
           Y+++V  +       E+   G ++LL  RF+DS    E V +A  ++  +N+ I+     
Sbjct: 11  YQEYVTRHPAATAQLETAVRGFSYLLAGRFADSHELSELVYSASNLLVLLNDGIL----- 70

Query: 64  QMHVNATVPSSFPYS---LCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 123
           +  +   +P S         +S L+ +E  +E+ A + +G+  RW  IA+ +  K + R+
Sbjct: 71  RKELRKKLPVSLSQQKLLTWLSVLECVEVFMEMGAAKVWGEVGRWLVIALVQLAKAVLRM 130

Query: 124 ALFKNCGYKIVLDGGETI---NNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQW 183
            L     +K  L     I   + E   +     H P       G++   Y G  +     
Sbjct: 131 LLL--LWFKAGLQTSPPIVPLDRETQAQPPDGDHSP-------GNHEQSYVGKRSNRVVR 190

Query: 184 NLEGRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHRGA 243
            L+         +G        P    G Q QH     +  TP               G 
Sbjct: 191 TLQNTPSLHSRHWG-------APQQREGRQQQHHEELSATPTP--------------LGL 250

Query: 244 LFVTGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSASTSRKNQRS 303
                E L+I RPL+++L +  +G RSW PW L+  VD+   S LS            R 
Sbjct: 251 QETIAEFLYIARPLLHLLSLGLWGQRSWKPWLLAGVVDVTSLSLLS-----------DRK 310

Query: 304 CLSDSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVL-EPVPFVGFLTAKIVELI 363
            L+  E+ ELRRR +L  +YL+R PF++R++  ++    ++L + VP VG +T  +++ +
Sbjct: 311 GLTRRERRELRRRTILLLYYLLRSPFYDRFSEARILFLLQLLADHVPGVGLVTRPLMDYL 333

Query: 364 IGAQTRYTY 366
              Q  Y Y
Sbjct: 371 PTWQKIYFY 333

BLAST of Csor.00g256740 vs. ExPASy Swiss-Prot
Match: Q2KII7 (Peroxisomal membrane protein PEX16 OS=Bos taurus OX=9913 GN=PEX16 PE=2 SV=2)

HSP 1 Score: 92.8 bits (229), Expect = 8.6e-18
Identity = 92/367 (25.07%), Postives = 160/367 (43.60%), Query Frame = 0

Query: 4   YKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIETAPT 63
           Y+++V  +       E+   GL++LL  RF+DS    E V +A  ++  +N+ I+     
Sbjct: 11  YQEYVTRHPAATAQLETAVRGLSYLLAGRFADSHELSELVYSASNLLVLLNDGIL----- 70

Query: 64  QMHVNATVPSSFPYS---LCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 123
           +  +   +P S         +S L+ +E  +E+ A + +G+  RW  IA+ +  K + R+
Sbjct: 71  RKELRKKLPMSLSQQRLLTWLSVLECVEVFMEMGATKVWGEVGRWLVIALIQLAKAVLRM 130

Query: 124 ALFKNCGYKIVLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 183
            L     +K  L     I                A ++ G H+        +G  + +  
Sbjct: 131 FLL--IWFKAGLQTSPPI--------VPLDREIQAQSRDGDHS--------SGSQEQSYV 190

Query: 184 GRALSALSRFGEKARMISPPAWSYGVQHQH-GMFNPSATTPERPTLSTILSEQGHRGALF 243
           G+  + + R  +    +    W    Q +  G+    A TP               G   
Sbjct: 191 GKRSNRVVRTLQNTPSLHSRHWGAPQQREELGV----APTP--------------LGLQE 250

Query: 244 VTGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSASTSRKNQRSCL 303
              E L I RPL+++L +  +G RSWTPW LS  VD+   S LS            R  L
Sbjct: 251 TIAESLHIARPLLHLLSLGLWGQRSWTPWLLSGVVDVTSLSLLS-----------DRKGL 310

Query: 304 SDSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVL-EPVPFVGFLTAKIVELIIG 363
           +  E+ ELRRR +L  +YL+R PF++R++  K+    ++L + VP +G +T  +++ +  
Sbjct: 311 TRRERLELRRRTILLLYYLLRSPFYDRFSEAKILFLLQLLADHVPGIGLVTRPLMDYLPN 325

Query: 364 AQTRYTY 366
            Q  Y Y
Sbjct: 371 WQKIYFY 325

BLAST of Csor.00g256740 vs. NCBI nr
Match: KAG6574772.1 (Peroxisome biogenesis protein 16, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 743 bits (1919), Expect = 9.18e-271
Identity = 369/369 (100.00%), Postives = 369/369 (100.00%), Query Frame = 0

Query: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60
           MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET
Sbjct: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60

Query: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120
           APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL
Sbjct: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120

Query: 121 ALFKNCGYKIVLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180
           ALFKNCGYKIVLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE
Sbjct: 121 ALFKNCGYKIVLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180

Query: 181 GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHRGALFV 240
           GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHRGALFV
Sbjct: 181 GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHRGALFV 240

Query: 241 TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSASTSRKNQRSCLS 300
           TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSASTSRKNQRSCLS
Sbjct: 241 TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSASTSRKNQRSCLS 300

Query: 301 DSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQ 360
           DSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQ
Sbjct: 301 DSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQ 360

Query: 361 TRYTYMSAS 369
           TRYTYMSAS
Sbjct: 361 TRYTYMSAS 369

BLAST of Csor.00g256740 vs. NCBI nr
Match: XP_022959429.1 (peroxisome biogenesis protein 16-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 737 bits (1903), Expect = 2.52e-268
Identity = 366/369 (99.19%), Postives = 367/369 (99.46%), Query Frame = 0

Query: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60
           MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET
Sbjct: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60

Query: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120
           APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL
Sbjct: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120

Query: 121 ALFKNCGYKIVLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180
           ALFKNCGYKI+LDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE
Sbjct: 121 ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180

Query: 181 GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHRGALFV 240
           GRALSALSRFGEKARMIS PAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHRGALFV
Sbjct: 181 GRALSALSRFGEKARMISAPAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHRGALFV 240

Query: 241 TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSASTSRKNQRSCLS 300
           TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGT FLSYATSASTSRKNQRSCLS
Sbjct: 241 TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTCFLSYATSASTSRKNQRSCLS 300

Query: 301 DSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQ 360
           DSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQ
Sbjct: 301 DSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQ 360

Query: 361 TRYTYMSAS 369
           TRYTYMSAS
Sbjct: 361 TRYTYMSAS 369

BLAST of Csor.00g256740 vs. NCBI nr
Match: XP_023006235.1 (peroxisome biogenesis protein 16-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 732 bits (1889), Expect = 3.44e-266
Identity = 362/369 (98.10%), Postives = 364/369 (98.64%), Query Frame = 0

Query: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60
           MEAYKKW RDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET
Sbjct: 1   MEAYKKWARDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60

Query: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120
           APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL
Sbjct: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120

Query: 121 ALFKNCGYKIVLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180
           ALFKNCGYKI+LDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHD WNLE
Sbjct: 121 ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDHWNLE 180

Query: 181 GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHRGALFV 240
           GRALSALSRFGE ARMISPPAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHRGALFV
Sbjct: 181 GRALSALSRFGENARMISPPAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHRGALFV 240

Query: 241 TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSASTSRKNQRSCLS 300
           TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSY TSASTSRKNQRSCLS
Sbjct: 241 TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYGTSASTSRKNQRSCLS 300

Query: 301 DSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQ 360
           DSEKDELR+RKMLWAFYLMRDPFFERYTRRKLEGAEKVL PVPFVGFLTAKIVELIIGAQ
Sbjct: 301 DSEKDELRKRKMLWAFYLMRDPFFERYTRRKLEGAEKVLAPVPFVGFLTAKIVELIIGAQ 360

Query: 361 TRYTYMSAS 369
           TRYTYMSAS
Sbjct: 361 TRYTYMSAS 369

BLAST of Csor.00g256740 vs. NCBI nr
Match: XP_023547844.1 (peroxisome biogenesis protein 16-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 731 bits (1887), Expect = 6.94e-266
Identity = 363/369 (98.37%), Postives = 364/369 (98.64%), Query Frame = 0

Query: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60
           MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET
Sbjct: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60

Query: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120
           APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL
Sbjct: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120

Query: 121 ALFKNCGYKIVLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180
           ALFKNCGYKI+LDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGY GYLNG D WNLE
Sbjct: 121 ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYPGYLNGQDHWNLE 180

Query: 181 GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHRGALFV 240
           GRALSALSRFGE ARMISPPAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHRGALFV
Sbjct: 181 GRALSALSRFGENARMISPPAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHRGALFV 240

Query: 241 TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSASTSRKNQRSCLS 300
           TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSASTSRKNQRSCLS
Sbjct: 241 TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSASTSRKNQRSCLS 300

Query: 301 DSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQ 360
           DSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVP VGFLTAKIVELIIGAQ
Sbjct: 301 DSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPLVGFLTAKIVELIIGAQ 360

Query: 361 TRYTYMSAS 369
           TRYTYMSAS
Sbjct: 361 TRYTYMSAS 369

BLAST of Csor.00g256740 vs. NCBI nr
Match: XP_038876429.1 (peroxisome biogenesis protein 16 isoform X1 [Benincasa hispida])

HSP 1 Score: 699 bits (1803), Expect = 4.41e-253
Identity = 345/369 (93.50%), Postives = 356/369 (96.48%), Query Frame = 0

Query: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60
           MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGI+TAVNEHIIET
Sbjct: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIITAVNEHIIET 60

Query: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120
            PTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEA+KVLFRL
Sbjct: 61  TPTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL 120

Query: 121 ALFKNCGYKIVLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180
           ALFKN GYKI+L+GGET NNERHL+TSTSQHR NAFTKHGGH+ASG+SGYLNGH+QWNLE
Sbjct: 121 ALFKNSGYKILLEGGETTNNERHLETSTSQHRANAFTKHGGHHASGFSGYLNGHNQWNLE 180

Query: 181 GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHRGALFV 240
           GRALSALSRFGE ARMISPPAWSY VQHQH + NP AT PERPTLSTILSEQGH GALFV
Sbjct: 181 GRALSALSRFGENARMISPPAWSYRVQHQHAILNPPATIPERPTLSTILSEQGHHGALFV 240

Query: 241 TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSASTSRKNQRSCLS 300
           TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSAS SRK +RSCLS
Sbjct: 241 TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSASASRKGRRSCLS 300

Query: 301 DSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQ 360
           DSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELI+GAQ
Sbjct: 301 DSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIVGAQ 360

Query: 361 TRYTYMSAS 369
           TRYTYMSAS
Sbjct: 361 TRYTYMSAS 369

BLAST of Csor.00g256740 vs. ExPASy TrEMBL
Match: A0A6J1H817 (Peroxisomal membrane protein PEX16 OS=Cucurbita moschata OX=3662 GN=LOC111460410 PE=3 SV=1)

HSP 1 Score: 737 bits (1903), Expect = 1.22e-268
Identity = 366/369 (99.19%), Postives = 367/369 (99.46%), Query Frame = 0

Query: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60
           MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET
Sbjct: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60

Query: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120
           APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL
Sbjct: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120

Query: 121 ALFKNCGYKIVLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180
           ALFKNCGYKI+LDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE
Sbjct: 121 ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180

Query: 181 GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHRGALFV 240
           GRALSALSRFGEKARMIS PAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHRGALFV
Sbjct: 181 GRALSALSRFGEKARMISAPAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHRGALFV 240

Query: 241 TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSASTSRKNQRSCLS 300
           TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGT FLSYATSASTSRKNQRSCLS
Sbjct: 241 TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTCFLSYATSASTSRKNQRSCLS 300

Query: 301 DSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQ 360
           DSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQ
Sbjct: 301 DSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQ 360

Query: 361 TRYTYMSAS 369
           TRYTYMSAS
Sbjct: 361 TRYTYMSAS 369

BLAST of Csor.00g256740 vs. ExPASy TrEMBL
Match: A0A6J1KZL2 (Peroxisomal membrane protein PEX16 OS=Cucurbita maxima OX=3661 GN=LOC111499027 PE=3 SV=1)

HSP 1 Score: 732 bits (1889), Expect = 1.66e-266
Identity = 362/369 (98.10%), Postives = 364/369 (98.64%), Query Frame = 0

Query: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60
           MEAYKKW RDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET
Sbjct: 1   MEAYKKWARDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60

Query: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120
           APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL
Sbjct: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120

Query: 121 ALFKNCGYKIVLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180
           ALFKNCGYKI+LDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHD WNLE
Sbjct: 121 ALFKNCGYKILLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDHWNLE 180

Query: 181 GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHRGALFV 240
           GRALSALSRFGE ARMISPPAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHRGALFV
Sbjct: 181 GRALSALSRFGENARMISPPAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHRGALFV 240

Query: 241 TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSASTSRKNQRSCLS 300
           TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSY TSASTSRKNQRSCLS
Sbjct: 241 TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYGTSASTSRKNQRSCLS 300

Query: 301 DSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQ 360
           DSEKDELR+RKMLWAFYLMRDPFFERYTRRKLEGAEKVL PVPFVGFLTAKIVELIIGAQ
Sbjct: 301 DSEKDELRKRKMLWAFYLMRDPFFERYTRRKLEGAEKVLAPVPFVGFLTAKIVELIIGAQ 360

Query: 361 TRYTYMSAS 369
           TRYTYMSAS
Sbjct: 361 TRYTYMSAS 369

BLAST of Csor.00g256740 vs. ExPASy TrEMBL
Match: A0A1S3CCB9 (Peroxisomal membrane protein PEX16 OS=Cucumis melo OX=3656 GN=LOC103499046 PE=3 SV=1)

HSP 1 Score: 686 bits (1771), Expect = 1.61e-248
Identity = 338/369 (91.60%), Postives = 356/369 (96.48%), Query Frame = 0

Query: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60
           MEAY+KWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGI+TAVNEHIIET
Sbjct: 1   MEAYRKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIITAVNEHIIET 60

Query: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120
           APTQMHVNAT+PSSFPYSLCI+ALKNLETLIEVAAQQYYGDDKRWNFIAVTEA+KVLFRL
Sbjct: 61  APTQMHVNATMPSSFPYSLCIAALKNLETLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL 120

Query: 121 ALFKNCGYKIVLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180
           ALFKN GYKI+L+GGET NNERHL+TSTSQH+ NAFTKHGGH+ASG+SGYLNGH+ WNLE
Sbjct: 121 ALFKNSGYKILLEGGETTNNERHLETSTSQHKANAFTKHGGHHASGFSGYLNGHNPWNLE 180

Query: 181 GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHRGALFV 240
           GRALSALSRFGE A+MISPPAWS+ VQHQH + NP AT PERPTLSTILSEQGHRGALF+
Sbjct: 181 GRALSALSRFGENAQMISPPAWSHRVQHQHAILNPPATIPERPTLSTILSEQGHRGALFI 240

Query: 241 TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSASTSRKNQRSCLS 300
           TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSAS SRK+QR  LS
Sbjct: 241 TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSASASRKDQRLRLS 300

Query: 301 DSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQ 360
           DSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELI+GAQ
Sbjct: 301 DSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIVGAQ 360

Query: 361 TRYTYMSAS 369
           TRYTYMSAS
Sbjct: 361 TRYTYMSAS 369

BLAST of Csor.00g256740 vs. ExPASy TrEMBL
Match: A0A0A0K8X7 (Peroxisomal membrane protein PEX16 OS=Cucumis sativus OX=3659 GN=Csa_6G075160 PE=3 SV=1)

HSP 1 Score: 684 bits (1764), Expect = 1.88e-247
Identity = 337/369 (91.33%), Postives = 352/369 (95.39%), Query Frame = 0

Query: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60
           MEAY+KWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGI+TAVNEHIIET
Sbjct: 1   MEAYRKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIITAVNEHIIET 60

Query: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRL 120
           APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEA+KVLFRL
Sbjct: 61  APTQMHVNATVPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRL 120

Query: 121 ALFKNCGYKIVLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLE 180
           ALFKN GYKI+L+GGET N+ERHL+TSTS H+ NAFTKHGGH+ASG+SG LNGH  WNLE
Sbjct: 121 ALFKNSGYKILLEGGETTNSERHLETSTSHHKANAFTKHGGHHASGFSGDLNGHSPWNLE 180

Query: 181 GRALSALSRFGEKARMISPPAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHRGALFV 240
           GRALSALSRFGE ARM SPPAWSY VQHQH + NP AT PERPTLSTILSEQGHRGALF+
Sbjct: 181 GRALSALSRFGENARMTSPPAWSYRVQHQHAILNPPATIPERPTLSTILSEQGHRGALFI 240

Query: 241 TGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSASTSRKNQRSCLS 300
           TGEVLFI RPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSAS SRK+QR CLS
Sbjct: 241 TGEVLFIARPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSASASRKDQRPCLS 300

Query: 301 DSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQ 360
           DSEKDELRRRKMLWAFYLMRDPFFERYTR+KLEGAEKVLEPVPFVGFLTAKIVELI+GAQ
Sbjct: 301 DSEKDELRRRKMLWAFYLMRDPFFERYTRQKLEGAEKVLEPVPFVGFLTAKIVELIVGAQ 360

Query: 361 TRYTYMSAS 369
           TRYTYMSAS
Sbjct: 361 TRYTYMSAS 369

BLAST of Csor.00g256740 vs. ExPASy TrEMBL
Match: A0A5D3DLT0 (Peroxisomal membrane protein PEX16 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00240 PE=3 SV=1)

HSP 1 Score: 639 bits (1647), Expect = 6.94e-230
Identity = 316/346 (91.33%), Postives = 333/346 (96.24%), Query Frame = 0

Query: 24  GLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIETAPTQMHVNATVPSSFPYSLCISA 83
           GLTWLLPERFSDSEIGPEAVSAAVGI+TAVNEHIIETAPTQMHVNAT+PSSFPYSLCI+A
Sbjct: 8   GLTWLLPERFSDSEIGPEAVSAAVGIITAVNEHIIETAPTQMHVNATMPSSFPYSLCIAA 67

Query: 84  LKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLFRLALFKNCGYKIVLDGGETINNERH 143
           LKNLETLIEVAAQQYYGDDKRWNFIAVTEA+KVLFRLALFKN GYKI+L+GGET NNERH
Sbjct: 68  LKNLETLIEVAAQQYYGDDKRWNFIAVTEAMKVLFRLALFKNSGYKILLEGGETTNNERH 127

Query: 144 LKTSTSQHRPNAFTKHGGHNASGYSGYLNGHDQWNLEGRALSALSRFGEKARMISPPAWS 203
           L+TSTSQH+ NAFTKHGGH+ASG+SGYLNGH+ WNLEGRALSALSRFGE A+MISPPAWS
Sbjct: 128 LETSTSQHKANAFTKHGGHHASGFSGYLNGHNPWNLEGRALSALSRFGENAQMISPPAWS 187

Query: 204 YGVQHQHGMFNPSATTPERPTLSTILSEQGHRGALFVTGEVLFITRPLIYVLLIRKYGSR 263
           + VQHQH + NP AT PERPTLSTILSEQGHRGALF+TGEVLFITRPLIYVLLIRKYGSR
Sbjct: 188 HRVQHQHAILNPPATIPERPTLSTILSEQGHRGALFITGEVLFITRPLIYVLLIRKYGSR 247

Query: 264 SWTPWFLSLAVDLLGTSFLSYATSASTSRKNQRSCLSDSEKDELRRRKMLWAFYLMRDPF 323
           SWTPWFLSLAVDLLGTSFLSYATSAS SRK+QR  LSDSEKDELRRRKMLWAFYLMRDPF
Sbjct: 248 SWTPWFLSLAVDLLGTSFLSYATSASASRKDQRLRLSDSEKDELRRRKMLWAFYLMRDPF 307

Query: 324 FERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIIGAQTRYTYMSAS 369
           FERYTRRKLEGAEKVLEPVPFVGFLTAKIVELI+GAQTRYTYMSAS
Sbjct: 308 FERYTRRKLEGAEKVLEPVPFVGFLTAKIVELIVGAQTRYTYMSAS 353

BLAST of Csor.00g256740 vs. TAIR 10
Match: AT2G45690.1 (shrunken seed protein (SSE1) )

HSP 1 Score: 391.3 bits (1004), Expect = 8.3e-109
Identity = 210/374 (56.15%), Postives = 265/374 (70.86%), Query Frame = 0

Query: 1   MEAYKKWVRDNRDYLHSFESLANGLTWLLPERFSDSEIGPEAVSAAVGIVTAVNEHIIET 60
           MEAYK+WV  NR+Y+ SF S ANGLTWLLPE+FS SEIGPEAV+A +GI + +NEHIIE 
Sbjct: 1   MEAYKQWVWRNREYVQSFGSFANGLTWLLPEKFSASEIGPEAVTAFLGIFSTINEHIIEN 60

Query: 61  APT-QMHVNAT-VPSSFPYSLCISALKNLETLIEVAAQQYYGDDKRWNFIAVTEALKVLF 120
           APT + HV ++    S  Y L I+ LK+LET++EVAA+ +YG DK+WN+I +TEA+K + 
Sbjct: 61  APTPRGHVGSSGNDPSLSYPLLIAILKDLETVVEVAAEHFYG-DKKWNYIILTEAMKAVI 120

Query: 121 RLALFKNCGYKIVLDGGETINNERHLKTSTSQHRPNAFTKHGGHNASGYS-GYLNGHDQW 180
           RLALF+N GYK++L GGET N E+    S SQ+R      + G N   +  G  N H+ W
Sbjct: 121 RLALFRNSGYKMLLQGGETPNEEKDSNQSESQNRAG----NSGRNLGPHGLGNQNHHNPW 180

Query: 181 NLEGRALSALSRFGEKARMI--SPPAWSYGVQHQHGMFNPSATTPERPTLSTILSEQGHR 240
           NLEGRA+SALS FG+ AR    S P WS  +QHQ  +  P      R T+S +L+E+G  
Sbjct: 181 NLEGRAMSALSSFGQNARTTTSSTPGWSRRIQHQQAVIEPPMIKERRRTMSELLTEKGVN 240

Query: 241 GALFVTGEVLFITRPLIYVLLIRKYGSRSWTPWFLSLAVDLLGTSFLSYATSASTSRKNQ 300
           GALF  GEVL+ITRPLIYVL IRKYG RSW PW +SL+VD LG   L  A S     K++
Sbjct: 241 GALFAIGEVLYITRPLIYVLFIRKYGVRSWIPWAISLSVDTLGMGLL--ANSKWWGEKSK 300

Query: 301 RSCLSDSEKDELRRRKMLWAFYLMRDPFFERYTRRKLEGAEKVLEPVPFVGFLTAKIVEL 360
           +   S  EKDELRRRK++WA YLMRDPFF +YTR+KLE ++K LE +P +GFLT KIVEL
Sbjct: 301 QVHFSGPEKDELRRRKLIWALYLMRDPFFTKYTRQKLESSQKKLELIPLIGFLTEKIVEL 360

Query: 361 IIGAQTRYTYMSAS 370
           + GAQ+RYTY+S S
Sbjct: 361 LEGAQSRYTYISGS 367

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8S8S11.2e-10756.15Peroxisome biogenesis protein 16 OS=Arabidopsis thaliana OX=3702 GN=PEX16 PE=1 S... [more]
Q91XC94.6e-1924.80Peroxisomal membrane protein PEX16 OS=Mus musculus OX=10090 GN=Pex16 PE=1 SV=2[more]
B0JYZ27.8e-1925.48Peroxisomal membrane protein PEX16 OS=Xenopus tropicalis OX=8364 GN=pex16 PE=2 S... [more]
Q9Y5Y51.7e-1824.93Peroxisomal membrane protein PEX16 OS=Homo sapiens OX=9606 GN=PEX16 PE=1 SV=2[more]
Q2KII78.6e-1825.07Peroxisomal membrane protein PEX16 OS=Bos taurus OX=9913 GN=PEX16 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
KAG6574772.19.18e-271100.00Peroxisome biogenesis protein 16, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022959429.12.52e-26899.19peroxisome biogenesis protein 16-like isoform X1 [Cucurbita moschata][more]
XP_023006235.13.44e-26698.10peroxisome biogenesis protein 16-like isoform X1 [Cucurbita maxima][more]
XP_023547844.16.94e-26698.37peroxisome biogenesis protein 16-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_038876429.14.41e-25393.50peroxisome biogenesis protein 16 isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1H8171.22e-26899.19Peroxisomal membrane protein PEX16 OS=Cucurbita moschata OX=3662 GN=LOC111460410... [more]
A0A6J1KZL21.66e-26698.10Peroxisomal membrane protein PEX16 OS=Cucurbita maxima OX=3661 GN=LOC111499027 P... [more]
A0A1S3CCB91.61e-24891.60Peroxisomal membrane protein PEX16 OS=Cucumis melo OX=3656 GN=LOC103499046 PE=3 ... [more]
A0A0A0K8X71.88e-24791.33Peroxisomal membrane protein PEX16 OS=Cucumis sativus OX=3659 GN=Csa_6G075160 PE... [more]
A0A5D3DLT06.94e-23091.33Peroxisomal membrane protein PEX16 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
Match NameE-valueIdentityDescription
AT2G45690.18.3e-10956.15shrunken seed protein (SSE1) [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013919Peroxisome membrane protein, Pex16PFAMPF08610Pex16coord: 2..360
e-value: 2.1E-78
score: 264.1
IPR013919Peroxisome membrane protein, Pex16PANTHERPTHR13299UNCHARACTERIZEDcoord: 1..369

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g256740.m01Csor.00g256740.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007031 peroxisome organization
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005778 peroxisomal membrane