Csor.00g252850 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g252850
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionthermospermine synthase ACAULIS5-like
LocationCsor_Chr06: 9992900 .. 9995036 (+)
RNA-Seq ExpressionCsor.00g252850
SyntenyCsor.00g252850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGATGTTTCCTCTGCAAATTTGGTTAACAATGGTAATGAACTCATTGAGAAGGGTCATACCCTAAATGGGTATCGCAAGAGTTGTTGGTACGAGGAGGAGATCGAAGAGAATTTAAGATGGTCGTTTGCTCTTAATAGGTAGCTGTCCTGTTTTTTTGAATCCTCTGTTTATTTTCATTAATGTTCTTCAAGTTCTAAAAATTTTCGTTTTTTGAATATTTCAAGTGGGGTTCGTGAGACAAAATCATTAAACTATACTCTGTTGGTTCTTATGTTTGGATCTTTAAAAAGTTGAACACTTTTTTCCTTCCCACTTCAAGTCGAGCTCAATAAAATAATCCATTGGTCTTGGAAAAGACAGACAAGAAACCTTAAAACACAATAAAAACTTCAGTTTGGAGATTGTTTGTCTGCCAAAATCGACATACTTTGTAATCAACTGTTCTTGTTCTTTGTTGTTGTTATTGTTATCTCGTAGCATACTTCATACTGGCGCTTCCCAGTATCAAGACATCGCGCTACTCGACACAAAGCCGTTCGGGAAGGCGTTGGTGATCGACGGGAAGCTTCAAAGTGCAGAAACTGATGAGTTCATTTACCATGAATGCCTTGTTCATCCTTCACTTCTCCATCATCCCAAGTAATTTCATAGCTTAATGCTTCATTTTCTATAATCCGTAAGGAGAACGAAACATCCTTTATAAGGGTGTGGAAACTTTTCCCTGGCGTACGCGTTTTAAAGCCTTGAGGGGAAGTTTGAAAGGGAAAGCCCAAAGAAAACAATATCTACTAGCGGTGGGCCTGGGCCTAGGCCGTTACAGAATCAAACTATATAAAATGTTATTGAATCTTCTTGTTTGTAATGTTTTGAACTCAGTCCAAAGACAATCTTCATCATGGGAGGAGGTGAAGGCTCCACAGCAAGAGAGCTACTCCGACACAAGACAGTGGAGAAAGTTGTGATGTGCGATATCGACGAGGTTCGAATTCGTTTCGGTTTCTTCATCGTTTCCATTACTCAATACTAAGAATCTCTCTAACCCCATGATGGATTAACAATTGAACAGGAAGTTGTTGATTTCTGCAAGTCATATCTGATAGTAAACAAAGAAGCTTTCAGTGATCCAAGACTTGAACTTATCATAAACGATGCCAGGTTCGGTTTCGAGTAGCGGTTTTAACGACTCGTCTTGTGTTATGATTGCTGCTAATAAGTTCCATAATGTAGAGCGGAGCTCGAGAACCGAACGGAGCAATACGACGTGATCGTCGGCGATCTTGCTGACCCAATTGAGGGAGGTCCTTGTTATAAACTCTACACTAAGTCCTTCTATGAGTACACTGTGAAGCCTAGATTGAAACAAGATGGCATATTCATCACTCAGGTATTTGTGGGAGATGAAAATGGAAGAACTTAAGAGATTTTATATGAGATTTGAAGCTTAAATGCTTGTGGAAACGTATGCAGGCTGGCCCAGCTGGGATTTTCAGCCATACAGAAGTGTTTTCTTGCATCTATAACACCTTGAAGCAAGTTTTTAAATGTGAGCTAAACAAGGGTATCATTTGTATTGGTTTTAGGCTATGTTTCTTCAAGGAAACTAATGGATTGTTCATGTTTTTTGCTTCTAATTTGTAGATGTGGTGCCTTATTCAGCTCATATTCCTTCCTTTGCTGATACTTGGGGTTGGGTTATGGTAAGAAAAATGATAACTTTTCATGCTAAACGATTCGATCTCGAGGTTGTTCGGTTACATGGGAAAACGATAATTCGAACATGTTCTGTTCAGATCGGTTTCGAGTAAAGCTCTAAACTTTTGAATGTTTTAGGCATCCGACACTCCTTTTGTGCTGAATGCTGATGATTTAGACCAAAGAATCAAACAAAGAATCAAAGGGGAGAACAAGTACTTTGATGGAAAGACATTCTCATCGGCCTCGACGTTAAGCAAGGCGGTTCGGAAGTCGTAAGTGGTCGACATAGTTTTGGTTGTTGATGTTGTGATTGATTTGTGAATTTGACAAGAAGTTTTGTAGGCTCGACAACGAGACGCACGTGTACACGGAAGGAACGGCAAGGTTCATATATGGATATGGAGCTGCAGCAGCCTGCAAGCTGAACCAAGTTTGA

mRNA sequence

ATGGCGGATGTTTCCTCTGCAAATTTGGTTAACAATGGTAATGAACTCATTGAGAAGGGTCATACCCTAAATGGGTATCGCAAGAGTTGTTGGTACGAGGAGGAGATCGAAGAGAATTTAAGATGGTCGTTTGCTCTTAATAGCATACTTCATACTGGCGCTTCCCAGTATCAAGACATCGCGCTACTCGACACAAAGCCGTTCGGGAAGGCGTTGGTGATCGACGGGAAGCTTCAAAGTGCAGAAACTGATGAGTTCATTTACCATGAATGCCTTGTTCATCCTTCACTTCTCCATCATCCCAATCCAAAGACAATCTTCATCATGGGAGGAGGTGAAGGCTCCACAGCAAGAGAGCTACTCCGACACAAGACAGTGGAGAAAGTTGTGATGTGCGATATCGACGAGGAAGTTGTTGATTTCTGCAAGTCATATCTGATAGTAAACAAAGAAGCTTTCAGTGATCCAAGACTTGAACTTATCATAAACGATGCCAGAGCGGAGCTCGAGAACCGAACGGAGCAATACGACGTGATCGTCGGCGATCTTGCTGACCCAATTGAGGGAGGTCCTTGTTATAAACTCTACACTAAGTCCTTCTATGAGTACACTGTGAAGCCTAGATTGAAACAAGATGGCATATTCATCACTCAGGCTGGCCCAGCTGGGATTTTCAGCCATACAGAAGTGTTTTCTTGCATCTATAACACCTTGAAGCAAGTTTTTAAATATGTGGTGCCTTATTCAGCTCATATTCCTTCCTTTGCTGATACTTGGGGTTGGGTTATGGCATCCGACACTCCTTTTGTGCTGAATGCTGATGATTTAGACCAAAGAATCAAACAAAGAATCAAAGGGGAGAACAAGTACTTTGATGGAAAGACATTCTCATCGGCCTCGACGTTAAGCAAGGCGGTTCGGAAGTCGCTCGACAACGAGACGCACGTGTACACGGAAGGAACGGCAAGGTTCATATATGGATATGGAGCTGCAGCAGCCTGCAAGCTGAACCAAGTTTGA

Coding sequence (CDS)

ATGGCGGATGTTTCCTCTGCAAATTTGGTTAACAATGGTAATGAACTCATTGAGAAGGGTCATACCCTAAATGGGTATCGCAAGAGTTGTTGGTACGAGGAGGAGATCGAAGAGAATTTAAGATGGTCGTTTGCTCTTAATAGCATACTTCATACTGGCGCTTCCCAGTATCAAGACATCGCGCTACTCGACACAAAGCCGTTCGGGAAGGCGTTGGTGATCGACGGGAAGCTTCAAAGTGCAGAAACTGATGAGTTCATTTACCATGAATGCCTTGTTCATCCTTCACTTCTCCATCATCCCAATCCAAAGACAATCTTCATCATGGGAGGAGGTGAAGGCTCCACAGCAAGAGAGCTACTCCGACACAAGACAGTGGAGAAAGTTGTGATGTGCGATATCGACGAGGAAGTTGTTGATTTCTGCAAGTCATATCTGATAGTAAACAAAGAAGCTTTCAGTGATCCAAGACTTGAACTTATCATAAACGATGCCAGAGCGGAGCTCGAGAACCGAACGGAGCAATACGACGTGATCGTCGGCGATCTTGCTGACCCAATTGAGGGAGGTCCTTGTTATAAACTCTACACTAAGTCCTTCTATGAGTACACTGTGAAGCCTAGATTGAAACAAGATGGCATATTCATCACTCAGGCTGGCCCAGCTGGGATTTTCAGCCATACAGAAGTGTTTTCTTGCATCTATAACACCTTGAAGCAAGTTTTTAAATATGTGGTGCCTTATTCAGCTCATATTCCTTCCTTTGCTGATACTTGGGGTTGGGTTATGGCATCCGACACTCCTTTTGTGCTGAATGCTGATGATTTAGACCAAAGAATCAAACAAAGAATCAAAGGGGAGAACAAGTACTTTGATGGAAAGACATTCTCATCGGCCTCGACGTTAAGCAAGGCGGTTCGGAAGTCGCTCGACAACGAGACGCACGTGTACACGGAAGGAACGGCAAGGTTCATATATGGATATGGAGCTGCAGCAGCCTGCAAGCTGAACCAAGTTTGA

Protein sequence

MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSASTLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV
Homology
[Close]
BLAST of Csor.00g252850 vs. ExPASy Swiss-Prot
Match: Q9S7X6 (Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 1.4e-128
Identity = 206/311 (66.24%), Postives = 262/311 (84.24%), Query Frame = 0 

Query: 22  TLNGYRKSC-WYEEEIEENLRWSFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQS 81
           TL+  ++ C WYEE I+++L+WSFALNS+LH G S+YQDIALLDTK FGK LVIDGK+QS
Sbjct: 25  TLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDGKMQS 84

Query: 82  AETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVD 141
           AE DEFIYHECL+HP+LL HPNPKT+FIMGGGEGS ARE+L+H T+EKVVMCDID+EVVD
Sbjct: 85  AERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQEVVD 144

Query: 142 FCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIVGDLADPIEGGPCYKLYTKSF 201
           FC+ +L VN +AF + +LEL+I DA+AELE R E++D+IVGDLADP+EGGPCY+LYTKSF
Sbjct: 145 FCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGPCYQLYTKSF 204

Query: 202 YEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWG 261
           Y+  +KP+L  +GIF+TQAGPAGIF+H EVF+ IYNT+KQVFKYV  Y+AH+PSFADTWG
Sbjct: 205 YQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAHVPSFADTWG 264

Query: 262 WVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSASTLSKAVRKSLDNETHVYTEG 321
           WVMASD  F +  D++D+RI++R+ GE  Y +  +F SA+TL+K +  +L+ ET VY+E 
Sbjct: 265 WVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTISLALEKETEVYSEE 324

Query: 322 TARFIYGYGAA 332
            ARFI+G+G A
Sbjct: 325 NARFIHGHGVA 335

[Close]
BLAST of Csor.00g252850 vs. ExPASy Swiss-Prot
Match: Q975S5 (Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) OX=273063 GN=speE PE=3 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 6.0e-58
Identity = 118/277 (42.60%), Postives = 172/277 (62.09%), Query Frame = 0 

Query: 45  ALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPK 104
           A+  ++    + YQ   L++   FGKAL++DGK+QS  TDEFIYHE LVHP LL   NP+
Sbjct: 20  AITKVIAEEKTPYQRALLVELARFGKALILDGKIQSTITDEFIYHEALVHPLLLSINNPE 79

Query: 105 TIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIV-NKEAFSDPRLELIIN 164
            I I+GGGEG+T RE+L+HKT++ V M DID  V+DF K YL   ++ AF +P+ +L+I 
Sbjct: 80  KILILGGGEGATLREVLKHKTIKNVTMVDIDPVVIDFAKKYLQEWHQGAFDNPKSKLVIE 139

Query: 165 DARAELENRTEQYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAG 224
           D    ++   E++D +V DL DPI+  P   LYTK FYE     R+ + GI      P+ 
Sbjct: 140 DGYKFIKETKEKFDAVVIDLTDPIKDSPSQMLYTKEFYEEV--KRISKWGIVTQATSPS- 199

Query: 225 IFSHTEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWGWVMASD--TPFVLNADDLDQRIK 284
            FS  E FS IYNT+K VFK V     ++P+F   WG+V ASD   P   + ++++ RIK
Sbjct: 200 -FS-LETFSIIYNTIKHVFKKVSAGITYVPAFDGLWGFVYASDEVNPAEFSKEEINNRIK 259

Query: 285 QRIKGENKYFDGKTFSSASTLSKAVRKSLDNETHVYT 319
           +RI G  +++DG+T  +  ++ K +R+ L+ E  + T
Sbjct: 260 ERIDGSLRFYDGETHITMFSIPKHIREVLEKENKIST 291

[Close]
BLAST of Csor.00g252850 vs. ExPASy Swiss-Prot
Match: A2BJU2 (Polyamine aminopropyltransferase 2 OS=Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5) OX=415426 GN=speE2 PE=1 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 1.1e-56
Identity = 121/272 (44.49%), Postives = 170/272 (62.50%), Query Frame = 0 

Query: 49  ILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFI 108
           I   GA++YQ+  + +    GKALV+DGK+QS+  DE  YHE LVHP LL H  P+ + +
Sbjct: 27  IYAAGATRYQEYMIAELAGIGKALVLDGKVQSSILDEHWYHEALVHPILLAHQCPRKVLV 86

Query: 109 MGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIV-NKEAFSDPRLELIINDARA 168
           +GGGEG+T RE+LRH  VE V M DIDEE+V+  K +L   ++ AFS  +LEL+I D R 
Sbjct: 87  IGGGEGATVREVLRHSCVEHVTMVDIDEELVELAKKHLAEWHQGAFSSDKLELVIGDGRR 146

Query: 169 ELENRTEQYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSH 228
            +EN   +YD I+ DL DP+EGGP  +LYT  FY   VK  L+  G  +TQA    +   
Sbjct: 147 YVENCHRKYDAIILDLVDPMEGGPAARLYTLEFYR-AVKGCLRPGGAVVTQATSPTL--S 206

Query: 229 TEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWGWVMASDT--PFVLNADDLDQRIKQRIK 288
             V++ I NTL +VF  V PY +++ S+   WG+V ASDT  P  L+A ++D+ I  RI+
Sbjct: 207 PRVYAVIRNTLAKVFTIVRPYVSYVRSYNGLWGFVAASDTVDPAKLSAKEVDELIAARIR 266

Query: 289 GENKYFDGKTFSSASTLSKAVRKSLDNETHVY 318
           G+ +++DG+T      L   VR+ L +ET  Y
Sbjct: 267 GQLRFYDGETHEWMFRLPLPVRQVL-SETRDY 294

[Close]
BLAST of Csor.00g252850 vs. ExPASy Swiss-Prot
Match: Q9UXE4 (Polyamine aminopropyltransferase OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=speE PE=1 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.8e-54
Identity = 112/276 (40.58%), Postives = 165/276 (59.78%), Query Frame = 0 

Query: 46  LNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPN-PK 105
           +  +L    + YQ + L++   FGK L+IDGK+QS   DE IYHE LVHP LL     PK
Sbjct: 21  IEKVLAEEKTPYQHVTLVEFTRFGKGLIIDGKVQSTLYDEHIYHELLVHPLLLSLTKPPK 80

Query: 106 TIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIV-NKEAFSDPRLELIIN 165
           ++ I+GGGEG+T RE+L++K+VEK VM DIDE+V++F K YL   ++ AF D R  L+I 
Sbjct: 81  SVLILGGGEGATLREVLKYKSVEKAVMVDIDEKVIEFAKKYLYEWHQGAFEDKRTNLVIT 140

Query: 166 DARAELENRTEQYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAG 225
           D    ++   E+YD I+ DL DPI+    Y LYTK FYE  ++  L + G  +TQA    
Sbjct: 141 DGLKFIKETKEKYDAIILDLTDPIKNSTSYMLYTKEFYE-KLREILNERGGIVTQATSPS 200

Query: 226 IFSHTEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQR 285
                EV+  IYNT+K+VFK       ++ SF   WG+V     P +L+ D++D +I +R
Sbjct: 201 F--SLEVYVTIYNTIKEVFKEASASYTYMASFDGLWGFVYGGVRPDLLSEDEVDSKINER 260

Query: 286 IKGENKYFDGKTFSSASTLSKAVRKSLDNETHVYTE 320
           I G+ +++DG +   + +L K ++      T + TE
Sbjct: 261 IDGQLRFYDGYSHKISFSLPKNIKSEFQKITKISTE 293

[Close]
BLAST of Csor.00g252850 vs. ExPASy Swiss-Prot
Match: Q9YE02 (Polyamine aminopropyltransferase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) OX=272557 GN=speE PE=3 SV=2)

HSP 1 Score: 212.6 bits (540), Expect = 6.9e-54
Identity = 112/299 (37.46%), Postives = 184/299 (61.54%), Query Frame = 0 

Query: 26  YRKSCWYEEEIEENLRWSFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDE 85
           + K  W+ E +  +      +  ++  G S++Q+IA++     GK LV+DGK QS+E+DE
Sbjct: 10  FLKWSWFLEWLTPDRATLKHIEDVIFQGRSRFQEIAVVRVSGEGKVLVLDGKTQSSESDE 69

Query: 86  FIYHECLVHPSLLHHPNPKTIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSY 145
           F+YHE LVHP+++ H +P+ + I+GGGEG+T RE+L+H++VEK VM DIDE VV+  + +
Sbjct: 70  FMYHEALVHPAMILHGSPRKVLILGGGEGATLREVLKHRSVEKAVMVDIDETVVNVAREH 129

Query: 146 LIV-NKEAFSDPRLELIINDARAELENRTEQ-YDVIVGDLADPIEGGPCYKLYTKSFYEY 205
           L   ++ AF DPR E++I+DA   + ++ E  +DV++ DL DP+E GP  +LY++ +Y  
Sbjct: 130 LREWHRGAFDDPRAEVVIDDAWNYVASKAETGFDVVIADLVDPLEAGPATRLYSEEYYR- 189

Query: 206 TVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWGWVM 265
            VK  +   G+F+TQA    I   TE  + I NT+ +VFK+V  Y  ++PSF   WG+V+
Sbjct: 190 MVKDVMNPGGVFVTQA--VSISHLTEYHAIIRNTVARVFKHVESYGVYVPSFDSMWGFVV 249

Query: 266 ASD--TPFVL-NADDLDQRIKQRIKG-ENKYFDGKTFSSASTLSKAVRKSLDNETHVYT 319
           ASD   P +L +    + R+  +++G E ++ D  +      + K  R+++  E    T
Sbjct: 250 ASDDKDPRILGDRGFFETRLSHQLQGAELRFLDYASMLHMLNIPKMYREAIAREKRYAT 305

[Close]
BLAST of Csor.00g252850 vs. NCBI nr
Match: KAG6597450.1 (Thermospermine synthase ACAULIS5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 694 bits (1791), Expect = 2.87e-252
Identity = 339/339 (100.00%), Postives = 339/339 (100.00%), Query Frame = 0 

Query: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339
           TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV
Sbjct: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339

[Close]
BLAST of Csor.00g252850 vs. NCBI nr
Match: XP_022934423.1 (thermospermine synthase ACAULIS5-like [Cucurbita moschata])

HSP 1 Score: 692 bits (1786), Expect = 1.66e-251
Identity = 338/339 (99.71%), Postives = 339/339 (100.00%), Query Frame = 0 

Query: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYE+TVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEHTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339
           TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV
Sbjct: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339

[Close]
BLAST of Csor.00g252850 vs. NCBI nr
Match: XP_023539341.1 (thermospermine synthase ACAULIS5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 691 bits (1784), Expect = 3.35e-251
Identity = 337/339 (99.41%), Postives = 339/339 (100.00%), Query Frame = 0 

Query: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNP+TIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPRTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENR+EQYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRSEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339
           TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV
Sbjct: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339

[Close]
BLAST of Csor.00g252850 vs. NCBI nr
Match: XP_022973651.1 (thermospermine synthase ACAULIS5-like [Cucurbita maxima] >XP_022975154.1 thermospermine synthase ACAULIS5-like [Cucurbita maxima])

HSP 1 Score: 691 bits (1783), Expect = 4.75e-251
Identity = 338/339 (99.71%), Postives = 338/339 (99.71%), Query Frame = 0 

Query: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIK ENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKEENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339
           TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV
Sbjct: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339

[Close]
BLAST of Csor.00g252850 vs. NCBI nr
Match: XP_038880186.1 (thermospermine synthase ACAULIS5-like [Benincasa hispida])

HSP 1 Score: 676 bits (1744), Expect = 4.04e-245
Identity = 332/339 (97.94%), Postives = 334/339 (98.53%), Query Frame = 0 

Query: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHP+LLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAF DPRLELIINDARAELENRTE YDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRI GENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRITGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339
            LSKAVRKSLDNETHVYTEGTARFIYGYGAAA CKLNQV
Sbjct: 301 ILSKAVRKSLDNETHVYTEGTARFIYGYGAAA-CKLNQV 338

[Close]
BLAST of Csor.00g252850 vs. ExPASy TrEMBL
Match: A0A6J1F7M2 (thermospermine synthase ACAULIS5-like OS=Cucurbita moschata OX=3662 GN=LOC111441602 PE=3 SV=1)

HSP 1 Score: 692 bits (1786), Expect = 8.03e-252
Identity = 338/339 (99.71%), Postives = 339/339 (100.00%), Query Frame = 0 

Query: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYE+TVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEHTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339
           TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV
Sbjct: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339

[Close]
BLAST of Csor.00g252850 vs. ExPASy TrEMBL
Match: A0A6J1IJL1 (thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC111474176 PE=3 SV=1)

HSP 1 Score: 691 bits (1783), Expect = 2.30e-251
Identity = 338/339 (99.71%), Postives = 338/339 (99.71%), Query Frame = 0 

Query: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIK ENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKEENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339
           TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV
Sbjct: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339

[Close]
BLAST of Csor.00g252850 vs. ExPASy TrEMBL
Match: A0A6J1C9S2 (thermospermine synthase ACAULIS5-like OS=Momordica charantia OX=3673 GN=LOC111009509 PE=3 SV=1)

HSP 1 Score: 673 bits (1736), Expect = 3.24e-244
Identity = 329/339 (97.05%), Postives = 334/339 (98.53%), Query Frame = 0 

Query: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSS NLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSVNLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHP+LLHHPNPKTIFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKTIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAF DPRLEL+INDARAELE+R EQYDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELVINDARAELESREEQYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMAS+TPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASNTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339
           TLSKAVRKSLD ETHVYTEGTARFIYGYGAAA CKLNQV
Sbjct: 301 TLSKAVRKSLDTETHVYTEGTARFIYGYGAAA-CKLNQV 338

[Close]
BLAST of Csor.00g252850 vs. ExPASy TrEMBL
Match: A0A0A0L7M9 (Spermine synthase OS=Cucumis sativus OX=3659 GN=Csa_3G147770 PE=3 SV=1)

HSP 1 Score: 666 bits (1718), Expect = 1.80e-241
Identity = 328/339 (96.76%), Postives = 331/339 (97.64%), Query Frame = 0 

Query: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGH+LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHSLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHP+LLHHPNPK+IFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAF DPRLELIINDARAELENRTE YDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPF LNADDLDQRIKQRI GENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339
           TLSKAVRKSLDNET VYTE TARFIYGYGA A CKLNQV
Sbjct: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEA-CKLNQV 338

[Close]
BLAST of Csor.00g252850 vs. ExPASy TrEMBL
Match: A0A5D3D0P3 (Thermospermine synthase ACAULIS5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00350 PE=3 SV=1)

HSP 1 Score: 665 bits (1717), Expect = 2.55e-241
Identity = 328/339 (96.76%), Postives = 330/339 (97.35%), Query Frame = 0 

Query: 1   MADVSSANLVNNGNELIEKGHTLNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60
           MADVSSANLVNNGNELIEKGH LNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI
Sbjct: 1   MADVSSANLVNNGNELIEKGHALNGYRKSCWYEEEIEENLRWSFALNSILHTGASQYQDI 60

Query: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTAREL 120
           ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHP+LLHHPNPK+IFIMGGGEGSTAREL
Sbjct: 61  ALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPALLHHPNPKSIFIMGGGEGSTAREL 120

Query: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIV 180
           LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAF DPRLELIINDARAELENRTE YDVIV
Sbjct: 121 LRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFCDPRLELIINDARAELENRTELYDVIV 180

Query: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240
           GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ
Sbjct: 181 GDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQ 240

Query: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSAS 300
           VFKYVVPYSAHIPSFADTWGWVMASDTPF LNADDLDQRIKQRI GENKYFDGKTFSSAS
Sbjct: 241 VFKYVVPYSAHIPSFADTWGWVMASDTPFNLNADDLDQRIKQRITGENKYFDGKTFSSAS 300

Query: 301 TLSKAVRKSLDNETHVYTEGTARFIYGYGAAAACKLNQV 339
           TLSKAVRKSLDNET VYTE TARFIYGYGA A CKLNQV
Sbjct: 301 TLSKAVRKSLDNETQVYTEETARFIYGYGAEA-CKLNQV 338

[Close]
BLAST of Csor.00g252850 vs. TAIR 10
Match: AT5G19530.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 460.7 bits (1184), Expect = 1.0e-129
Identity = 206/311 (66.24%), Postives = 262/311 (84.24%), Query Frame = 0 

Query: 22  TLNGYRKSC-WYEEEIEENLRWSFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQS 81
           TL+  ++ C WYEE I+++L+WSFALNS+LH G S+YQDIALLDTK FGK LVIDGK+QS
Sbjct: 25  TLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDGKMQS 84

Query: 82  AETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVD 141
           AE DEFIYHECL+HP+LL HPNPKT+FIMGGGEGS ARE+L+H T+EKVVMCDID+EVVD
Sbjct: 85  AERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQEVVD 144

Query: 142 FCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIVGDLADPIEGGPCYKLYTKSF 201
           FC+ +L VN +AF + +LEL+I DA+AELE R E++D+IVGDLADP+EGGPCY+LYTKSF
Sbjct: 145 FCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGPCYQLYTKSF 204

Query: 202 YEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWG 261
           Y+  +KP+L  +GIF+TQAGPAGIF+H EVF+ IYNT+KQVFKYV  Y+AH+PSFADTWG
Sbjct: 205 YQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAHVPSFADTWG 264

Query: 262 WVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSASTLSKAVRKSLDNETHVYTEG 321
           WVMASD  F +  D++D+RI++R+ GE  Y +  +F SA+TL+K +  +L+ ET VY+E 
Sbjct: 265 WVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTISLALEKETEVYSEE 324

Query: 322 TARFIYGYGAA 332
            ARFI+G+G A
Sbjct: 325 NARFIHGHGVA 335

[Close]
BLAST of Csor.00g252850 vs. TAIR 10
Match: AT5G19530.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 460.7 bits (1184), Expect = 1.0e-129
Identity = 206/311 (66.24%), Postives = 262/311 (84.24%), Query Frame = 0 

Query: 22  TLNGYRKSC-WYEEEIEENLRWSFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQS 81
           TL+  ++ C WYEE I+++L+WSFALNS+LH G S+YQDIALLDTK FGK LVIDGK+QS
Sbjct: 18  TLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDGKMQS 77

Query: 82  AETDEFIYHECLVHPSLLHHPNPKTIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVD 141
           AE DEFIYHECL+HP+LL HPNPKT+FIMGGGEGS ARE+L+H T+EKVVMCDID+EVVD
Sbjct: 78  AERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQEVVD 137

Query: 142 FCKSYLIVNKEAFSDPRLELIINDARAELENRTEQYDVIVGDLADPIEGGPCYKLYTKSF 201
           FC+ +L VN +AF + +LEL+I DA+AELE R E++D+IVGDLADP+EGGPCY+LYTKSF
Sbjct: 138 FCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGPCYQLYTKSF 197

Query: 202 YEYTVKPRLKQDGIFITQAGPAGIFSHTEVFSCIYNTLKQVFKYVVPYSAHIPSFADTWG 261
           Y+  +KP+L  +GIF+TQAGPAGIF+H EVF+ IYNT+KQVFKYV  Y+AH+PSFADTWG
Sbjct: 198 YQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAHVPSFADTWG 257

Query: 262 WVMASDTPFVLNADDLDQRIKQRIKGENKYFDGKTFSSASTLSKAVRKSLDNETHVYTEG 321
           WVMASD  F +  D++D+RI++R+ GE  Y +  +F SA+TL+K +  +L+ ET VY+E 
Sbjct: 258 WVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTISLALEKETEVYSEE 317

Query: 322 TARFIYGYGAA 332
            ARFI+G+G A
Sbjct: 318 NARFIHGHGVA 328

[Close]
BLAST of Csor.00g252850 vs. TAIR 10
Match: AT1G23820.2 (spermidine synthase 1 )

HSP 1 Score: 157.5 bits (397), Expect = 1.9e-38
Identity = 80/225 (35.56%), Postives = 128/225 (56.89%), Query Frame = 0 

Query: 43  SFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPN 102
           S  +  +L  G S YQD+ +  +  +GK LV+DG +Q  E DE  Y E + H  L   PN
Sbjct: 105 SLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPN 164

Query: 103 PKTIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELII 162
           PK + ++GGG+G   RE+ RH ++E++ MC+ID+ VVD  K +       + DPR+ L+I
Sbjct: 165 PKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVI 224

Query: 163 NDARAELENRTE-QYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGP 222
            D  A L+N  E  YD ++ D +DPI  GP  +L+ K F++ +V   L+  G+  TQA  
Sbjct: 225 GDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKELFEKPFFQ-SVARALRPGGVVCTQA-- 284

Query: 223 AGIFSHTEVFSCIYNTLKQVFKYVVPYS-AHIPSFADTWG-WVMA 265
             ++ H ++   I +  +++FK  V Y+   +P++   WG W+ A
Sbjct: 285 ESLWLHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPRHWGHWIYA 324

[Close]
BLAST of Csor.00g252850 vs. TAIR 10
Match: AT1G23820.1 (spermidine synthase 1 )

HSP 1 Score: 157.1 bits (396), Expect = 2.4e-38
Identity = 87/280 (31.07%), Postives = 153/280 (54.64%), Query Frame = 0 

Query: 43  SFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPN 102
           S  +  +L  G S YQD+ +  +  +GK LV+DG +Q  E DE  Y E + H  L   PN
Sbjct: 105 SLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPN 164

Query: 103 PKTIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELII 162
           PK + ++GGG+G   RE+ RH ++E++ MC+ID+ VVD  K +       + DPR+ L+I
Sbjct: 165 PKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVI 224

Query: 163 NDARAELENRTE-QYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGP 222
            D  A L+N  E  YD ++ D +DPI  GP  +L+ K F++ +V   L+  G+  TQA  
Sbjct: 225 GDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKELFEKPFFQ-SVARALRPGGVVCTQA-- 284

Query: 223 AGIFSHTEVFSCIYNTLKQVFKYVVPYS-AHIPSF-ADTWGWVMAS----DTPFVLNADD 282
             ++ H ++   I +  +++FK  V Y+   +P++ +   G+++ S    D  F    + 
Sbjct: 285 ESLWLHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPSGVIGFMLCSTEGPDVDFKHPLNP 344

Query: 283 LDQRIKQRIKGENKYFDGKTFSSASTLSKAVRKSLDNETH 316
           +D+    +  G  K+++ +  S+A  L    +K ++++ +
Sbjct: 345 IDES-SSKSNGPLKFYNAEIHSAAFCLPSFAKKVIESKAN 378

[Close]
BLAST of Csor.00g252850 vs. TAIR 10
Match: AT1G70310.1 (spermidine synthase 2 )

HSP 1 Score: 153.7 bits (387), Expect = 2.7e-37
Identity = 93/283 (32.86%), Postives = 151/283 (53.36%), Query Frame = 0 

Query: 43  SFALNSILHTGASQYQDIALLDTKPFGKALVIDGKLQSAETDEFIYHECLVHPSLLHHPN 102
           S  +  IL  G S YQD+ +  +  +GK LV+DG +Q  E DE  Y E + H  L    N
Sbjct: 65  SLKVEKILFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSISN 124

Query: 103 PKTIFIMGGGEGSTARELLRHKTVEKVVMCDIDEEVVDFCKSYLIVNKEAFSDPRLELII 162
           PK + ++GGG+G   RE+ RH +VE++ +C+ID+ VVD  K Y       + DPR+ LII
Sbjct: 125 PKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDVAKQYFPNVAVGYEDPRVNLII 184

Query: 163 NDARAELENRTE-QYDVIVGDLADPIEGGPCYKLYTKSFYEYTVKPRLKQDGIFITQAGP 222
            D  A L+N  E  YD ++ D +DPI  GP  +L+ K F+E +V   L+  G+  TQA  
Sbjct: 185 GDGVAFLKNAAEGTYDAVIVDSSDPI--GPAKELFEKPFFE-SVNRALRPGGVVCTQA-- 244

Query: 223 AGIFSHTEVFSCIYNTLKQVFKYVVPYS-AHIPSF-ADTWGWVMAS------DTPFVLNA 282
             ++ H ++   I +  + +FK  V Y+   +P++ +   G+++ S      D    ++ 
Sbjct: 245 ESLWLHMDIIEDIVSNCRDIFKGSVNYAWTSVPTYPSGVIGFMLCSSEGPQVDFKKPVSL 304

Query: 283 DDLDQ-RIKQRIKGENKYFDGKTFSSASTLSKAVRKSLDNETH 316
            D D+  IK       KY++ +  S+A  L    +K +D++ +
Sbjct: 305 IDTDESSIKSHC--PLKYYNAEIHSAAFCLPSFAKKVIDSKAN 340

The following BLAST results are available for this feature:
BLAST of Csor.00g252850 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9S7X61.4e-12866.24Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV... [more]
Q975S56.0e-5842.60Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / ... [more]
A2BJU21.1e-5644.49Polyamine aminopropyltransferase 2 OS=Hyperthermus butylicus (strain DSM 5456 / ... [more]
Q9UXE41.8e-5440.58Polyamine aminopropyltransferase OS=Saccharolobus solfataricus (strain ATCC 3509... [more]
Q9YE026.9e-5437.46Polyamine aminopropyltransferase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 1... [more]
BLAST of Csor.00g252850 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG6597450.12.87e-252100.00Thermospermine synthase ACAULIS5, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022934423.11.66e-25199.71thermospermine synthase ACAULIS5-like [Cucurbita moschata][more]
XP_023539341.13.35e-25199.41thermospermine synthase ACAULIS5-like [Cucurbita pepo subsp. pepo][more]
XP_022973651.14.75e-25199.71thermospermine synthase ACAULIS5-like [Cucurbita maxima] >XP_022975154.1 thermos... [more]
XP_038880186.14.04e-24597.94thermospermine synthase ACAULIS5-like [Benincasa hispida][more]
BLAST of Csor.00g252850 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1F7M28.03e-25299.71thermospermine synthase ACAULIS5-like OS=Cucurbita moschata OX=3662 GN=LOC111441... [more]
A0A6J1IJL12.30e-25199.71thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC11147417... [more]
A0A6J1C9S23.24e-24497.05thermospermine synthase ACAULIS5-like OS=Momordica charantia OX=3673 GN=LOC11100... [more]
A0A0A0L7M91.80e-24196.76Spermine synthase OS=Cucumis sativus OX=3659 GN=Csa_3G147770 PE=3 SV=1[more]
A0A5D3D0P32.55e-24196.76Thermospermine synthase ACAULIS5-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
BLAST of Csor.00g252850 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G19530.11.0e-12966.24S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G19530.21.0e-12966.24S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G23820.21.9e-3835.56spermidine synthase 1 [more]
AT1G23820.12.4e-3831.07spermidine synthase 1 [more]
AT1G70310.12.7e-3732.86spermidine synthase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR037163Spermidine synthase, tetramerisation domain superfamilyGENE3D2.30.140.10Spermidine synthase, tetramerisation domaincoord: 29..81
e-value: 7.1E-15
score: 56.8
NoneNo IPR availablePFAMPF01564Spermine_synthcoord: 85..262
e-value: 2.1E-42
score: 144.6
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 82..334
e-value: 8.6E-78
score: 263.3
NoneNo IPR availablePANTHERPTHR43317:SF9SPERMINE SYNTHASEcoord: 1..331
NoneNo IPR availablePANTHERPTHR43317THERMOSPERMINE SYNTHASE ACAULIS5coord: 1..331
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 110..218
e-value: 6.80644E-7
score: 45.4987
IPR035246Spermidine synthase, tetramerisation domainPFAMPF17284Spermine_synt_Ncoord: 31..82
e-value: 2.5E-13
score: 49.7
IPR030374Polyamine biosynthesis domainPROSITEPS51006PABS_2coord: 29..266
score: 96.014542
IPR001045Spermidine/spermine synthasesHAMAPMF_00198Spermidine_synthcoord: 30..311
score: 31.273483
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 29..311

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g252850.m01Csor.00g252850.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006596 polyamine biosynthetic process
molecular_function GO:0010487 thermospermine synthase activity
molecular_function GO:0003824 catalytic activity