Csor.00g252240 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g252240
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationCsor_Chr06: 10324554 .. 10325872 (+)
RNA-Seq ExpressionCsor.00g252240
SyntenyCsor.00g252240
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCCAGTGCCTAGTTCTCCCGTCAACGTAGGACACATCGATGATGTGCAAGAACTCAGGAAGTTTAAGCCTAACACTATTCCCGAAAGATTTATCAGGGGCGTACAAGAGAGGCCAACACCAACAGCTTTAATCTCATCCTCTGATCTTCCCACTATTGATTTATCCAAACTTTTAAAAGGAAACAGAGATGAAATATTGCAGCTAGCCTCTGCCTGCGAGGAATGGGGATTTTTTCAGGTAAAGACTAATCGAATATTCTCTTTTCAGTCAAAGCTTAAGAACAGGCAATGGAAACTAAGGCCTTGGTAATCTTTTAGGTAATTAATCATGGAATTGCTCTAAACCTACTAGAAAGCATTGAGGGGGAGGCAATGGAATTCTTCGGGCTACCTTTGGAGGAAAAGCAGAAGTACCGAATGGCACCGGGCACAGTTCAAGGATACGGGCAAGCTTTTGTGTTCTCAGAGCACCAAAAGTTGGATTGGTGTAACATGTTTGCTCTGGGGGTTATACCCGACTCTATAAGAAACCCACTATTATGGCCAAAGAAGCCAACAAACTTCAGGTGGTTTTTTGTAATTCTAATGTCACCATTGAATTAACTGTTCAGCTACTTACAATCATACACCATTTTGCAGCAATACTGTTGAGATTTACTCTAAAGAAGCAAGAAAGCTTTGCCAGAATCTATTGAAGTACATAGCTTTAGGCCTTGGCCTAAAAGAGGACGTATTTGAAAAGGCGTTTGGGGTGGCCGTACAGGCAATAAGGATGAATTACTATCCACCATGTTCAAGACCTGATCTTGTTTTGGGGCTGAGCCCACACTCCGATGGAAGTGCTTTAACTGTTTTGCAGCAAGGAAAGGGCAACTCAGTAGGGCTTCAGATCCTTAAGGACGACAAGTGGGTTCCTGTTCAACCTCTCCCAAATGCACTTGTGATCAACATTGGCGATACAATGGAGGTGTGAATGAAACTTTGTCTAAATAAAGAAATGATTTTTTATCTTACAACAGAGACTTGACTCCTTTCTCATTGATTTATATGCAGGTTGTTACAAATGGCAGGTACAAAAGCGTGGAGCACCGAGCTGTGACTCACAAGCAGACTGACCGACTTTCGATAGTCACATTTTATGCACCTAGCTATGACATAGAGCTTGGACCAATGCCAGAGTTCGTGGATAAAAACAATCCATGCAAATATAGAAGATATAGTCATGGAGAATACAGTAAACACTATGTCACAAACAAGTTACAAGGAAAGAAAACCCTGGAGTTCGCGAAGATTTCAACCAAAAACTCCTACTAA

mRNA sequence

ATGGCTCCAGTGCCTAGTTCTCCCGTCAACGTAGGACACATCGATGATGTGCAAGAACTCAGGAAGTTTAAGCCTAACACTATTCCCGAAAGATTTATCAGGGGCGTACAAGAGAGGCCAACACCAACAGCTTTAATCTCATCCTCTGATCTTCCCACTATTGATTTATCCAAACTTTTAAAAGGAAACAGAGATGAAATATTGCAGCTAGCCTCTGCCTGCGAGGAATGGGGATTTTTTCAGGTAATTAATCATGGAATTGCTCTAAACCTACTAGAAAGCATTGAGGGGGAGGCAATGGAATTCTTCGGGCTACCTTTGGAGGAAAAGCAGAAGTACCGAATGGCACCGGGCACAGTTCAAGGATACGGGCAAGCTTTTGTGTTCTCAGAGCACCAAAAGTTGGATTGGTGTAACATGTTTGCTCTGGGGGTTATACCCGACTCTATAAGAAACCCACTATTATGGCCAAAGAAGCCAACAAACTTCAGCAATACTGTTGAGATTTACTCTAAAGAAGCAAGAAAGCTTTGCCAGAATCTATTGAAGTACATAGCTTTAGGCCTTGGCCTAAAAGAGGACGTATTTGAAAAGGCGTTTGGGGTGGCCGTACAGGCAATAAGGATGAATTACTATCCACCATGTTCAAGACCTGATCTTGTTTTGGGGCTGAGCCCACACTCCGATGGAAGTGCTTTAACTGTTTTGCAGCAAGGAAAGGGCAACTCAGTAGGGCTTCAGATCCTTAAGGACGACAAGTGGGTTCCTGTTCAACCTCTCCCAAATGCACTTGTGATCAACATTGGCGATACAATGGAGGTTGTTACAAATGGCAGGTACAAAAGCGTGGAGCACCGAGCTGTGACTCACAAGCAGACTGACCGACTTTCGATAGTCACATTTTATGCACCTAGCTATGACATAGAGCTTGGACCAATGCCAGAGTTCGTGGATAAAAACAATCCATGCAAATATAGAAGATATAGTCATGGAGAATACAGTAAACACTATGTCACAAACAAGTTACAAGGAAAGAAAACCCTGGAGTTCGCGAAGATTTCAACCAAAAACTCCTACTAA

Coding sequence (CDS)

ATGGCTCCAGTGCCTAGTTCTCCCGTCAACGTAGGACACATCGATGATGTGCAAGAACTCAGGAAGTTTAAGCCTAACACTATTCCCGAAAGATTTATCAGGGGCGTACAAGAGAGGCCAACACCAACAGCTTTAATCTCATCCTCTGATCTTCCCACTATTGATTTATCCAAACTTTTAAAAGGAAACAGAGATGAAATATTGCAGCTAGCCTCTGCCTGCGAGGAATGGGGATTTTTTCAGGTAATTAATCATGGAATTGCTCTAAACCTACTAGAAAGCATTGAGGGGGAGGCAATGGAATTCTTCGGGCTACCTTTGGAGGAAAAGCAGAAGTACCGAATGGCACCGGGCACAGTTCAAGGATACGGGCAAGCTTTTGTGTTCTCAGAGCACCAAAAGTTGGATTGGTGTAACATGTTTGCTCTGGGGGTTATACCCGACTCTATAAGAAACCCACTATTATGGCCAAAGAAGCCAACAAACTTCAGCAATACTGTTGAGATTTACTCTAAAGAAGCAAGAAAGCTTTGCCAGAATCTATTGAAGTACATAGCTTTAGGCCTTGGCCTAAAAGAGGACGTATTTGAAAAGGCGTTTGGGGTGGCCGTACAGGCAATAAGGATGAATTACTATCCACCATGTTCAAGACCTGATCTTGTTTTGGGGCTGAGCCCACACTCCGATGGAAGTGCTTTAACTGTTTTGCAGCAAGGAAAGGGCAACTCAGTAGGGCTTCAGATCCTTAAGGACGACAAGTGGGTTCCTGTTCAACCTCTCCCAAATGCACTTGTGATCAACATTGGCGATACAATGGAGGTTGTTACAAATGGCAGGTACAAAAGCGTGGAGCACCGAGCTGTGACTCACAAGCAGACTGACCGACTTTCGATAGTCACATTTTATGCACCTAGCTATGACATAGAGCTTGGACCAATGCCAGAGTTCGTGGATAAAAACAATCCATGCAAATATAGAAGATATAGTCATGGAGAATACAGTAAACACTATGTCACAAACAAGTTACAAGGAAAGAAAACCCTGGAGTTCGCGAAGATTTCAACCAAAAACTCCTACTAA

Protein sequence

MAPVPSSPVNVGHIDDVQELRKFKPNTIPERFIRGVQERPTPTALISSSDLPTIDLSKLLKGNRDEILQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGTVQGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQNLLKYIALGLGLKEDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGKGNSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIVTFYAPSYDIELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKISTKNSY
Homology
BLAST of Csor.00g252240 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 2.5e-78
Identity = 146/346 (42.20%), Postives = 217/346 (62.72%), Query Frame = 0

Query: 14  IDDVQELRKFKP-NTIPERFIRGVQER-PTPTALISSSDLPTIDLSKLLKGNR--DEILQ 73
           +  VQE+ K K   T+P R++R  Q++           ++P ID+ +L        E+ +
Sbjct: 14  VPSVQEMVKEKTITTVPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDSEVEK 73

Query: 74  LASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGTVQGYGQAFVF 133
           L  AC+EWGFFQ++NHGI  + L+ ++ E  +FF LP+EEK+K+   P  ++G+GQAFV 
Sbjct: 74  LDFACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQAFVV 133

Query: 134 SEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQNLLKYIALGL 193
           SE QKLDW ++F   V P  +R P L+PK P  F +T+E+YS E + + + L+  +A  L
Sbjct: 134 SEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKMARAL 193

Query: 194 GLKEDVFEKAFG--VAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGKGNSVGLQ 253
            +K +  EK F    +VQ++RMNYYPPC +PD V+GL+PHSD   LTVL Q   +  GLQ
Sbjct: 194 EIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQ-VNDVEGLQ 253

Query: 254 ILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIVTFYAPSYD 313
           I KD KWVPV+PLPNA ++NIGD +E++TNG Y+S+EHR V + + +RLSI TF+     
Sbjct: 254 IKKDGKWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHNVGMY 313

Query: 314 IELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKI 354
            E+GP    V++    +++R +  EY+    +  L GK  L+  +I
Sbjct: 314 KEVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

BLAST of Csor.00g252240 vs. ExPASy Swiss-Prot
Match: D4N501 (Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=3469 GN=DIOX2 PE=2 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 2.9e-71
Identity = 139/347 (40.06%), Postives = 204/347 (58.79%), Query Frame = 0

Query: 14  IDDVQELRKFKPNTIPERFIRGVQERPTPTALISSSD---LPTIDLSKLLKG----NRDE 73
           I  VQEL K     IP R+I  V+    P       D   +P ID+  L+       + E
Sbjct: 15  IPSVQELAKLTLAEIPSRYICTVENLQLPVGASVIDDHETVPVIDIENLISSEPVTEKLE 74

Query: 74  ILQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGTVQGYGQA 133
           + +L SAC+EWGFFQV+NHG+  +L+++++ +   FF L + EK KY    G V+G+GQA
Sbjct: 75  LDRLHSACKEWGFFQVVNHGVDTSLVDNVKSDIQGFFNLSMNEKIKYGQKDGDVEGFGQA 134

Query: 134 FVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQNLLKYIA 193
           FV SE Q LDW ++F +  +P  +R P L+ K P     T+E YS E +KL   L + + 
Sbjct: 135 FVASEDQTLDWADIFMILTLPLHLRKPHLFSKLPLPLRETIESYSSEMKKLSMVLFEKME 194

Query: 194 LGL---GLKEDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGKGNS 253
             L    ++     + F    Q +RMNYYPPC +P+L +GL+PHSD   LT+L Q     
Sbjct: 195 KALQVQAVEIKEISEVFKDMTQVMRMNYYPPCPQPELAIGLTPHSDFGGLTILLQ-LNEV 254

Query: 254 VGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIVTFYA 313
            GLQI  + +W+ V+PLPNA V+N+GD +E++TNG Y+SV+HRAV +   +RLSI TF+ 
Sbjct: 255 EGLQIKNEGRWISVKPLPNAFVVNVGDVLEIMTNGMYRSVDHRAVVNSTKERLSIATFHD 314

Query: 314 PSYDIELGPMPEFVDKNNPCKYRRYS-HGEYSKHYVTNKLQGKKTLE 350
           P+ + E+GP+   +  N P  +R  S +GE  + + + KL GK  L+
Sbjct: 315 PNLESEIGPISSLITPNTPALFRSGSTYGELVEEFHSRKLDGKSFLD 360

BLAST of Csor.00g252240 vs. ExPASy Swiss-Prot
Match: Q94LP4 (2-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD11 PE=1 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.1e-70
Identity = 134/346 (38.73%), Postives = 205/346 (59.25%), Query Frame = 0

Query: 11  VGHIDDVQELRKFKPNTIPERFIR-GVQERPTPTALISSSDLPTIDLSKLL--KGNRDEI 70
           V ++  + E+       IPER+IR                 +P IDL KLL  + + +E 
Sbjct: 12  VPNVQALAEICNDPDEHIPERYIRPEASSEEVINNYQGDMAIPIIDLKKLLCPQSSEEEC 71

Query: 71  LQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGTVQGYGQAF 130
           ++L SAC+ WGFF +INHG+   ++ +++ + ++FF  PL+ K++Y   P +++GYGQ+F
Sbjct: 72  VKLRSACQYWGFFLLINHGVPDEVIANLKRDIVDFFSQPLDTKKEYTQLPNSLEGYGQSF 131

Query: 131 VFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQNLLKYIAL 190
           VFSE QKLDW +M  L V P   R+   WP  P +F  +++ YS E + L   L +++A 
Sbjct: 132 VFSEDQKLDWADMLYLHVHPSDSRDLRFWPTSPASFRQSIDAYSSETKSLALCLFEFMAK 191

Query: 191 GLGLKEDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGKGNSVGLQ 250
            +G K +     F    + +RM YYPPC + D V+GLSPHSD   LT+L +   N  GLQ
Sbjct: 192 AVGAKPESLLDLFEEQPRGLRMAYYPPCRQADKVMGLSPHSDAGGLTLLLE-INNVQGLQ 251

Query: 251 ILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIVTFYAPSYD 310
           I KD KW  +     AL+ NIGDT+E+++NG+++SVEHRAV +   +R+S   F+ PS +
Sbjct: 252 IKKDGKWFSIDAPNGALIANIGDTLEILSNGKFRSVEHRAVINPNKERISAALFHYPSEN 311

Query: 311 IELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKI 354
           + + P+PEFV K+   KYR  S+ ++ K   T +L GK  +E  K+
Sbjct: 312 MVISPLPEFV-KDGKVKYRSISYLDFMKQIFTQQLDGKNRVEVLKL 355

BLAST of Csor.00g252240 vs. ExPASy Swiss-Prot
Match: O80449 (Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 2.5e-70
Identity = 128/325 (39.38%), Postives = 194/325 (59.69%), Query Frame = 0

Query: 14  IDDVQELRKFKPNTIPERFIRGVQERP--TPTALISSSDLPTIDLSKLLKGNRDEILQLA 73
           I  VQ L +    T+P R+++   +RP    T   +  ++P +D++ +  G  + +  + 
Sbjct: 9   IVSVQSLSQTGVPTVPNRYVKPAHQRPVFNTTQSDAGIEIPVLDMNDVW-GKPEGLRLVR 68

Query: 74  SACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGTVQGYGQAFVFSE 133
           SACEEWGFFQ++NHG+  +L+E + G   EFF LPLEEK+KY  +P T +GYG      +
Sbjct: 69  SACEEWGFFQMVNHGVTHSLMERVRGAWREFFELPLEEKRKYANSPDTYEGYGSRLGVVK 128

Query: 134 HQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQNLLKYIALGLGL 193
             KLDW + F L  +P SIRNP  WP +P      +E Y +E RKLC+ L + ++  LGL
Sbjct: 129 DAKLDWSDYFFLNYLPSSIRNPSKWPSQPPKIRELIEKYGEEVRKLCERLTETLSESLGL 188

Query: 194 KEDVFEKAFGVAVQ---AIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGKGNSVGLQI 253
           K +   +A G   +   ++R N+YP C +P L LGLS HSD   +T+L   +    GLQ+
Sbjct: 189 KPNKLMQALGGGDKVGASLRTNFYPKCPQPQLTLGLSSHSDPGGITILLPDE-KVAGLQV 248

Query: 254 LKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIVTFYAPSYDI 313
            + D WV ++ +PNAL++NIGD +++++NG YKSVEH+ + +   +R+S+  FY P  DI
Sbjct: 249 RRGDGWVTIKSVPNALIVNIGDQLQILSNGIYKSVEHQVIVNSGMERVSLAFFYNPRSDI 308

Query: 314 ELGPMPEFVDKNNPCKYRRYSHGEY 334
            +GP+ E V  N P  Y+     EY
Sbjct: 309 PVGPIEELVTANRPALYKPIRFDEY 331

BLAST of Csor.00g252240 vs. ExPASy Swiss-Prot
Match: D4N502 (Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 1.6e-69
Identity = 140/352 (39.77%), Postives = 209/352 (59.38%), Query Frame = 0

Query: 14  IDDVQELRKFKPNTIPERFIRGVQERPTPTALISSSD---LPTIDLSKLLKG----NRDE 73
           I  VQEL K     IP R+     E P      S +D   +P IDL  LL       + E
Sbjct: 15  IPSVQELAKLTLAEIPSRY-TCTGESPLNNIGASVTDDETVPVIDLQNLLSPEPVVGKLE 74

Query: 74  ILQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGTVQGYGQA 133
           + +L SAC+EWGFFQ++NHG+   L+++I+ E   FF LP+ EK KY    G  +G+GQ 
Sbjct: 75  LDKLHSACKEWGFFQLVNHGVDALLMDNIKSEIKGFFNLPMNEKTKYGQQDGDFEGFGQP 134

Query: 134 FVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQNLLKYIA 193
           ++ SE Q+LDW  +F++  +P  +R P L+P+ P  F  T+E Y  + +KL   + + + 
Sbjct: 135 YIESEDQRLDWTEVFSMLSLPLHLRKPHLFPELPLPFRETLESYLSKMKKLSTVVFEMLE 194

Query: 194 LGLGLKE-----DVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGKG 253
             L L E     D+FE      +Q +RMNYYPPC RP+LVLGL+ HSD S LT+L Q   
Sbjct: 195 KSLQLVEIKGMTDLFED----GLQTMRMNYYPPCPRPELVLGLTSHSDFSGLTILLQ-LN 254

Query: 254 NSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIVTF 313
              GLQI K+++W+ ++PLP+A ++N+GD +E++TNG Y+SVEHRAV +   +RLSI TF
Sbjct: 255 EVEGLQIRKEERWISIKPLPDAFIVNVGDILEIMTNGIYRSVEHRAVVNSTKERLSIATF 314

Query: 314 YAPSYDIELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKI 354
           +    + E+GP+   V    P  ++R  + +  K  ++ KL GK  L++ ++
Sbjct: 315 HDSKLESEIGPISSLVTPETPALFKRGRYEDILKENLSRKLDGKSFLDYMRM 360

BLAST of Csor.00g252240 vs. NCBI nr
Match: KAG6597511.1 (Protein SRG1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 733 bits (1891), Expect = 7.79e-267
Identity = 359/359 (100.00%), Postives = 359/359 (100.00%), Query Frame = 0

Query: 1   MAPVPSSPVNVGHIDDVQELRKFKPNTIPERFIRGVQERPTPTALISSSDLPTIDLSKLL 60
           MAPVPSSPVNVGHIDDVQELRKFKPNTIPERFIRGVQERPTPTALISSSDLPTIDLSKLL
Sbjct: 1   MAPVPSSPVNVGHIDDVQELRKFKPNTIPERFIRGVQERPTPTALISSSDLPTIDLSKLL 60

Query: 61  KGNRDEILQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGTV 120
           KGNRDEILQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGTV
Sbjct: 61  KGNRDEILQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGTV 120

Query: 121 QGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQN 180
           QGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQN
Sbjct: 121 QGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQN 180

Query: 181 LLKYIALGLGLKEDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGK 240
           LLKYIALGLGLKEDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGK
Sbjct: 181 LLKYIALGLGLKEDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGK 240

Query: 241 GNSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIVT 300
           GNSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIVT
Sbjct: 241 GNSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIVT 300

Query: 301 FYAPSYDIELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKISTKNSY 359
           FYAPSYDIELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKISTKNSY
Sbjct: 301 FYAPSYDIELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKISTKNSY 359

BLAST of Csor.00g252240 vs. NCBI nr
Match: XP_022936789.1 (protein SRG1-like [Cucurbita moschata] >KAG7028968.1 Protein SRG1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 731 bits (1886), Expect = 4.51e-266
Identity = 358/359 (99.72%), Postives = 358/359 (99.72%), Query Frame = 0

Query: 1   MAPVPSSPVNVGHIDDVQELRKFKPNTIPERFIRGVQERPTPTALISSSDLPTIDLSKLL 60
           MAPVPSSPVNVGHIDDVQELRKFKPNTIPERFIRGVQERPTPTALISSSDLPTIDLSKLL
Sbjct: 1   MAPVPSSPVNVGHIDDVQELRKFKPNTIPERFIRGVQERPTPTALISSSDLPTIDLSKLL 60

Query: 61  KGNRDEILQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGTV 120
           KGNRDEILQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGTV
Sbjct: 61  KGNRDEILQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGTV 120

Query: 121 QGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQN 180
           QGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQN
Sbjct: 121 QGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQN 180

Query: 181 LLKYIALGLGLKEDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGK 240
           LLKYIALGLGL EDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGK
Sbjct: 181 LLKYIALGLGLNEDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGK 240

Query: 241 GNSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIVT 300
           GNSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIVT
Sbjct: 241 GNSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIVT 300

Query: 301 FYAPSYDIELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKISTKNSY 359
           FYAPSYDIELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKISTKNSY
Sbjct: 301 FYAPSYDIELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKISTKNSY 359

BLAST of Csor.00g252240 vs. NCBI nr
Match: XP_022973772.1 (protein SRG1-like [Cucurbita maxima])

HSP 1 Score: 726 bits (1874), Expect = 3.16e-264
Identity = 358/360 (99.44%), Postives = 358/360 (99.44%), Query Frame = 0

Query: 1   MAPVPSSPVNVGHIDDVQELRKFKPNTIPERFIRGVQERPTP-TALISSSDLPTIDLSKL 60
           MAPVPSSPVNVGHIDDVQELRKFKPNTIPERFIRGVQERPTP TALISSSDLPTIDLSKL
Sbjct: 1   MAPVPSSPVNVGHIDDVQELRKFKPNTIPERFIRGVQERPTPPTALISSSDLPTIDLSKL 60

Query: 61  LKGNRDEILQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGT 120
           LKGNRDEILQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGT
Sbjct: 61  LKGNRDEILQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGT 120

Query: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQ 180
           VQGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQ
Sbjct: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQ 180

Query: 181 NLLKYIALGLGLKEDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240
           NLLKYIALGLGLKEDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG
Sbjct: 181 NLLKYIALGLGLKEDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240

Query: 241 KGNSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIV 300
           KGNSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIV
Sbjct: 241 KGNSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIV 300

Query: 301 TFYAPSYDIELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKISTKNSY 359
           TFYAPSYDIELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKI TKNSY
Sbjct: 301 TFYAPSYDIELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKIPTKNSY 360

BLAST of Csor.00g252240 vs. NCBI nr
Match: XP_023539447.1 (protein SRG1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 720 bits (1858), Expect = 8.68e-262
Identity = 355/360 (98.61%), Postives = 356/360 (98.89%), Query Frame = 0

Query: 1   MAPVPSSPVNVGHIDDVQELRKFKPNTIPERFIRGVQERPTP-TALISSSDLPTIDLSKL 60
           MAPVPSSPVNVGHIDDVQELRKFKPNTIPERFIRGVQERPTP TALISSSDLPTIDLSKL
Sbjct: 1   MAPVPSSPVNVGHIDDVQELRKFKPNTIPERFIRGVQERPTPPTALISSSDLPTIDLSKL 60

Query: 61  LKGNRDEILQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGT 120
           LKGNRDEILQLA ACEEWGFFQVINHGIA NLLESIEGEAMEFFGLPLEEKQKYRMAPGT
Sbjct: 61  LKGNRDEILQLAYACEEWGFFQVINHGIAQNLLESIEGEAMEFFGLPLEEKQKYRMAPGT 120

Query: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQ 180
           VQGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQ
Sbjct: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQ 180

Query: 181 NLLKYIALGLGLKEDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240
           NLLKYIALGLGLKEDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG
Sbjct: 181 NLLKYIALGLGLKEDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240

Query: 241 KGNSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIV 300
           KGNSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIV
Sbjct: 241 KGNSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIV 300

Query: 301 TFYAPSYDIELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKISTKNSY 359
           TFYAPSYDIELGPMP+FVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKI TKNSY
Sbjct: 301 TFYAPSYDIELGPMPKFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKIPTKNSY 360

BLAST of Csor.00g252240 vs. NCBI nr
Match: XP_038905272.1 (protein SRG1 [Benincasa hispida])

HSP 1 Score: 684 bits (1764), Expect = 1.85e-247
Identity = 331/359 (92.20%), Postives = 348/359 (96.94%), Query Frame = 0

Query: 1   MAPVPSSPVNVGHIDDVQELRKFKPNTIPERFIRGVQERPTP-TALISSSDLPTIDLSKL 60
           MAPVP SP+NVGHIDDVQELRKFKPNTIPERFIR +QERPTP T LISSSD+PTIDLSKL
Sbjct: 1   MAPVPISPINVGHIDDVQELRKFKPNTIPERFIRDIQERPTPATPLISSSDIPTIDLSKL 60

Query: 61  LKGNRDEILQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGT 120
           LKGNRDEILQL +ACEEWGFFQVINHGI+LNLLE+IEG+A+EFF LPLEEKQKY MAPGT
Sbjct: 61  LKGNRDEILQLTTACEEWGFFQVINHGISLNLLENIEGQALEFFRLPLEEKQKYAMAPGT 120

Query: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQ 180
           VQGYGQAFVFSEHQKLDWCNM ALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKE RKLC+
Sbjct: 121 VQGYGQAFVFSEHQKLDWCNMLALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEVRKLCK 180

Query: 181 NLLKYIALGLGLKEDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240
           NLLKYIALGLGLKE++FE+AFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG
Sbjct: 181 NLLKYIALGLGLKENLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240

Query: 241 KGNSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIV 300
           KG+SVGLQIL+DDKWVPVQP+PNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIV
Sbjct: 241 KGSSVGLQILRDDKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIV 300

Query: 301 TFYAPSYDIELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKISTKNS 358
           TFYAPSYDIELGPMPEFVDKNNPC YRRY+HGEYSKHYVTNKLQGK+TLEFAKI TKNS
Sbjct: 301 TFYAPSYDIELGPMPEFVDKNNPCMYRRYNHGEYSKHYVTNKLQGKRTLEFAKIPTKNS 359

BLAST of Csor.00g252240 vs. ExPASy TrEMBL
Match: A0A6J1F9A3 (protein SRG1-like OS=Cucurbita moschata OX=3662 GN=LOC111443268 PE=3 SV=1)

HSP 1 Score: 731 bits (1886), Expect = 2.18e-266
Identity = 358/359 (99.72%), Postives = 358/359 (99.72%), Query Frame = 0

Query: 1   MAPVPSSPVNVGHIDDVQELRKFKPNTIPERFIRGVQERPTPTALISSSDLPTIDLSKLL 60
           MAPVPSSPVNVGHIDDVQELRKFKPNTIPERFIRGVQERPTPTALISSSDLPTIDLSKLL
Sbjct: 1   MAPVPSSPVNVGHIDDVQELRKFKPNTIPERFIRGVQERPTPTALISSSDLPTIDLSKLL 60

Query: 61  KGNRDEILQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGTV 120
           KGNRDEILQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGTV
Sbjct: 61  KGNRDEILQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGTV 120

Query: 121 QGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQN 180
           QGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQN
Sbjct: 121 QGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQN 180

Query: 181 LLKYIALGLGLKEDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGK 240
           LLKYIALGLGL EDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGK
Sbjct: 181 LLKYIALGLGLNEDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGK 240

Query: 241 GNSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIVT 300
           GNSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIVT
Sbjct: 241 GNSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIVT 300

Query: 301 FYAPSYDIELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKISTKNSY 359
           FYAPSYDIELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKISTKNSY
Sbjct: 301 FYAPSYDIELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKISTKNSY 359

BLAST of Csor.00g252240 vs. ExPASy TrEMBL
Match: A0A6J1I8G0 (protein SRG1-like OS=Cucurbita maxima OX=3661 GN=LOC111472333 PE=3 SV=1)

HSP 1 Score: 726 bits (1874), Expect = 1.53e-264
Identity = 358/360 (99.44%), Postives = 358/360 (99.44%), Query Frame = 0

Query: 1   MAPVPSSPVNVGHIDDVQELRKFKPNTIPERFIRGVQERPTP-TALISSSDLPTIDLSKL 60
           MAPVPSSPVNVGHIDDVQELRKFKPNTIPERFIRGVQERPTP TALISSSDLPTIDLSKL
Sbjct: 1   MAPVPSSPVNVGHIDDVQELRKFKPNTIPERFIRGVQERPTPPTALISSSDLPTIDLSKL 60

Query: 61  LKGNRDEILQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGT 120
           LKGNRDEILQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGT
Sbjct: 61  LKGNRDEILQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGT 120

Query: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQ 180
           VQGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQ
Sbjct: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQ 180

Query: 181 NLLKYIALGLGLKEDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240
           NLLKYIALGLGLKEDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG
Sbjct: 181 NLLKYIALGLGLKEDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240

Query: 241 KGNSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIV 300
           KGNSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIV
Sbjct: 241 KGNSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIV 300

Query: 301 TFYAPSYDIELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKISTKNSY 359
           TFYAPSYDIELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKI TKNSY
Sbjct: 301 TFYAPSYDIELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKIPTKNSY 360

BLAST of Csor.00g252240 vs. ExPASy TrEMBL
Match: A0A5D3CXG4 (Protein SRG1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G00820 PE=3 SV=1)

HSP 1 Score: 674 bits (1739), Expect = 5.78e-244
Identity = 326/359 (90.81%), Postives = 342/359 (95.26%), Query Frame = 0

Query: 1   MAPVPSSPVNVGHIDDVQELRKFKPNTIPERFIRGVQERPTP-TALISSSDLPTIDLSKL 60
           MAPVP SP+ VGHIDDVQELRKFKPN IPERFIR +QERPTP T LISSSD+PTIDLSKL
Sbjct: 1   MAPVPISPIKVGHIDDVQELRKFKPNIIPERFIRDIQERPTPATPLISSSDIPTIDLSKL 60

Query: 61  LKGNRDEILQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGT 120
           LKGN+DEILQL +ACEEWGFFQVINHGIALNLLE+IEG+AMEFF LPLEEKQKY M PGT
Sbjct: 61  LKGNKDEILQLTTACEEWGFFQVINHGIALNLLENIEGQAMEFFRLPLEEKQKYAMVPGT 120

Query: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQ 180
           VQGYGQAFVFSEHQKLDWCNMFALGVIPD +RNPL WPKKP NFSNTVEIYSKE RKLC+
Sbjct: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDFLRNPLFWPKKPANFSNTVEIYSKEVRKLCK 180

Query: 181 NLLKYIALGLGLKEDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240
           NLLKYIALGLGLKED+FE+AFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG
Sbjct: 181 NLLKYIALGLGLKEDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240

Query: 241 KGNSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIV 300
           KG+SVGLQILKD KWVPVQP+PNALVINIGDTMEVVTNGRYKSVEHRAVTHK TDRLSIV
Sbjct: 241 KGSSVGLQILKDGKWVPVQPIPNALVINIGDTMEVVTNGRYKSVEHRAVTHKHTDRLSIV 300

Query: 301 TFYAPSYDIELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKISTKNS 358
           TFYAPSYDIELGPMPEFVDKNNPC+YRRY+HGEYSKHYVTNKLQGK+TLEFAKI TKNS
Sbjct: 301 TFYAPSYDIELGPMPEFVDKNNPCRYRRYNHGEYSKHYVTNKLQGKRTLEFAKIPTKNS 359

BLAST of Csor.00g252240 vs. ExPASy TrEMBL
Match: A0A6J1IXQ5 (protein SRG1-like OS=Cucurbita maxima OX=3661 GN=LOC111479417 PE=3 SV=1)

HSP 1 Score: 673 bits (1736), Expect = 1.66e-243
Identity = 327/359 (91.09%), Postives = 344/359 (95.82%), Query Frame = 0

Query: 1   MAPVPSSPVNVGHIDDVQELRKFKPNTIPERFIRGVQERPTP-TALISSSDLPTIDLSKL 60
           MAP P SP+NVGHIDDVQELRKFKPNTIP+RFIR +QERPT  TALISSSD+PTIDLSKL
Sbjct: 1   MAPAPISPINVGHIDDVQELRKFKPNTIPQRFIRDIQERPTQATALISSSDIPTIDLSKL 60

Query: 61  LKGNRDEILQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGT 120
           LKGNRDEIL L SACEEWGFFQVINHGIALNLLE+IEG+AMEFF LPLEEKQKY+MAPGT
Sbjct: 61  LKGNRDEILHLYSACEEWGFFQVINHGIALNLLENIEGKAMEFFRLPLEEKQKYQMAPGT 120

Query: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQ 180
           VQGYGQAFVFSEHQKLDWCNMFALGVIPDSIRN LLWPKKPTNFSNTVEIYSKE RKLCQ
Sbjct: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNNLLWPKKPTNFSNTVEIYSKEVRKLCQ 180

Query: 181 NLLKYIALGLGLKEDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240
           NLLKYIALGLGLK+D+FE+AFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGS LTVLQQG
Sbjct: 181 NLLKYIALGLGLKKDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSVLTVLQQG 240

Query: 241 KGNSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIV 300
           KG+SVGLQILKDDKWVPVQP+P+A VINIGDTMEVVTNGR+KSVEHRAVTHK +DRLSIV
Sbjct: 241 KGSSVGLQILKDDKWVPVQPIPDAFVINIGDTMEVVTNGRFKSVEHRAVTHKHSDRLSIV 300

Query: 301 TFYAPSYDIELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKISTKNS 358
           TFYAPSYDIELGPMPEFVDK NPCKYRRY+HGEYSKHYVTNKLQGK+TLEFAKI TKNS
Sbjct: 301 TFYAPSYDIELGPMPEFVDKKNPCKYRRYNHGEYSKHYVTNKLQGKRTLEFAKIPTKNS 359

BLAST of Csor.00g252240 vs. ExPASy TrEMBL
Match: A0A6J1GW69 (protein SRG1-like OS=Cucurbita moschata OX=3662 GN=LOC111457443 PE=3 SV=1)

HSP 1 Score: 670 bits (1729), Expect = 1.93e-242
Identity = 327/359 (91.09%), Postives = 344/359 (95.82%), Query Frame = 0

Query: 1   MAPVPSSPVNVGHIDDVQELRKFKPNTIPERFIRGVQERPTP-TALISSSDLPTIDLSKL 60
           MAPVP S +NVGHIDDVQELRKFKPNTIP+RFIR +QERPT  TALISSSD+PTIDLSKL
Sbjct: 1   MAPVPISTINVGHIDDVQELRKFKPNTIPQRFIRDIQERPTQATALISSSDIPTIDLSKL 60

Query: 61  LKGNRDEILQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGT 120
           LKGNRDEIL L SACEEWGFFQVINHGIALNLLE+IE +AMEFF LPLEEKQKY+MAPGT
Sbjct: 61  LKGNRDEILHLISACEEWGFFQVINHGIALNLLENIEEKAMEFFRLPLEEKQKYQMAPGT 120

Query: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQ 180
           VQGYGQAFVFSEHQKLDWCNMFALGVIPDSIRN LLWPKKPTNFSNTVEIYSKE RKLCQ
Sbjct: 121 VQGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNNLLWPKKPTNFSNTVEIYSKEVRKLCQ 180

Query: 181 NLLKYIALGLGLKEDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240
           NLLKYIALGLGLK+D+FE+AFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG
Sbjct: 181 NLLKYIALGLGLKKDLFEEAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQG 240

Query: 241 KGNSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIV 300
           KG+SVGLQILKDDKWVPVQP+P+A VINIGDTMEVVTNGR+KSVEHRAVTHK +DRLSIV
Sbjct: 241 KGSSVGLQILKDDKWVPVQPIPDAFVINIGDTMEVVTNGRFKSVEHRAVTHKHSDRLSIV 300

Query: 301 TFYAPSYDIELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKISTKNS 358
           TFYAPSYDIELGPMPEFVDK NPCKYRRY+HGEYSKHYVTNKLQGK+TLEFAKI TKNS
Sbjct: 301 TFYAPSYDIELGPMPEFVDKKNPCKYRRYNHGEYSKHYVTNKLQGKRTLEFAKIPTKNS 359

BLAST of Csor.00g252240 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 509.6 bits (1311), Expect = 2.0e-144
Identity = 247/362 (68.23%), Postives = 293/362 (80.94%), Query Frame = 0

Query: 1   MAPVPSSPVNVGHIDDVQELRKFKPNTIPERFIRGVQERPTPTALISSSDL----PTIDL 60
           MAP+P S + VG IDDVQEL K KPN +PERFIR   ER    + + +  L    P IDL
Sbjct: 1   MAPLPISSIRVGKIDDVQELIKSKPNKVPERFIREEYERGVVVSSLKTHHLHHQIPVIDL 60

Query: 61  SKLLKGNRD----EILQLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQK 120
           SKL K + D    EIL+L+ ACE+WGFFQVINHGI + ++E IE  A EFF +PLEEK+K
Sbjct: 61  SKLSKPDNDDFFFEILKLSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDMPLEEKKK 120

Query: 121 YRMAPGTVQGYGQAFVFSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSK 180
           Y M PGTVQGYGQAF+FSE QKLDWCNMFALGV P  IRNP LWP KP  FS ++E YSK
Sbjct: 121 YPMEPGTVQGYGQAFIFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARFSESLEGYSK 180

Query: 181 EARKLCQNLLKYIALGLGLKEDVFEKAFGVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSA 240
           E R+LC+ LLKYIA+ LGLKE+ FE+ FG AVQA+RMNYYPPCS PDLVLGLSPHSDGSA
Sbjct: 181 EIRELCKRLLKYIAISLGLKEERFEEMFGEAVQAVRMNYYPPCSSPDLVLGLSPHSDGSA 240

Query: 241 LTVLQQGKGNSVGLQILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQ 300
           LTVLQQ K + VGLQILKD+ WVPV+PLPNALVINIGDT+EV++NG+YKSVEHRAVT+++
Sbjct: 241 LTVLQQSKNSCVGLQILKDNTWVPVKPLPNALVINIGDTIEVLSNGKYKSVEHRAVTNRE 300

Query: 301 TDRLSIVTFYAPSYDIELGPMPEFV-DKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFA 354
            +RL+IVTFYAP+Y++E+ PM E V D+ NPCKYR Y+HG+YS HYV+NKLQGKK+L+FA
Sbjct: 301 KERLTIVTFYAPNYEVEIEPMSELVDDETNPCKYRSYNHGDYSYHYVSNKLQGKKSLDFA 360

BLAST of Csor.00g252240 vs. TAIR 10
Match: AT1G17020.1 (senescence-related gene 1 )

HSP 1 Score: 293.9 bits (751), Expect = 1.8e-79
Identity = 146/346 (42.20%), Postives = 217/346 (62.72%), Query Frame = 0

Query: 14  IDDVQELRKFKP-NTIPERFIRGVQER-PTPTALISSSDLPTIDLSKLLKGNR--DEILQ 73
           +  VQE+ K K   T+P R++R  Q++           ++P ID+ +L        E+ +
Sbjct: 14  VPSVQEMVKEKTITTVPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDSEVEK 73

Query: 74  LASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGTVQGYGQAFVF 133
           L  AC+EWGFFQ++NHGI  + L+ ++ E  +FF LP+EEK+K+   P  ++G+GQAFV 
Sbjct: 74  LDFACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQAFVV 133

Query: 134 SEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQNLLKYIALGL 193
           SE QKLDW ++F   V P  +R P L+PK P  F +T+E+YS E + + + L+  +A  L
Sbjct: 134 SEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKMARAL 193

Query: 194 GLKEDVFEKAFG--VAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGKGNSVGLQ 253
            +K +  EK F    +VQ++RMNYYPPC +PD V+GL+PHSD   LTVL Q   +  GLQ
Sbjct: 194 EIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQ-VNDVEGLQ 253

Query: 254 ILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIVTFYAPSYD 313
           I KD KWVPV+PLPNA ++NIGD +E++TNG Y+S+EHR V + + +RLSI TF+     
Sbjct: 254 IKKDGKWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHNVGMY 313

Query: 314 IELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKI 354
            E+GP    V++    +++R +  EY+    +  L GK  L+  +I
Sbjct: 314 KEVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

BLAST of Csor.00g252240 vs. TAIR 10
Match: AT4G25310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 285.8 bits (730), Expect = 4.8e-77
Identity = 145/345 (42.03%), Postives = 213/345 (61.74%), Query Frame = 0

Query: 14  IDDVQELRKFKPNT--IPERFIRGVQERPTPTALISSSDLPTIDLSKLLKGNR--DEILQ 73
           +  VQE+ K K  T  +P R++R  QE+         + +P ID+S L        EI +
Sbjct: 13  VPSVQEMVKEKVITTVLPPRYVRSDQEKGEAAIDSGENQIPIIDMSLLSSSTSMDSEIDK 72

Query: 74  LASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGTVQGYGQAFVF 133
           L  AC+EWGFFQ++NHG+ L+  +S   +  +FF LP+EEK+K    PG ++G+GQAFVF
Sbjct: 73  LDFACKEWGFFQLVNHGMDLDKFKS---DIQDFFNLPMEEKKKLWQQPGDIEGFGQAFVF 132

Query: 134 SEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQNLLKYIALGL 193
           SE QKLDW ++F L + P  +R P L+PK P  F +T++ YS E + + + L   +A  L
Sbjct: 133 SEEQKLDWADVFFLTMQPVPLRKPHLFPKLPLPFRDTLDTYSAELKSIAKVLFAKLASAL 192

Query: 194 GLKEDVFEKAFGVAV-QAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGKGNSVGLQI 253
            +K +  EK F   + Q IRMNYYPPC  PD  +GL+PHSD + LT+L Q      GLQI
Sbjct: 193 KIKPEEMEKLFDDELGQRIRMNYYPPCPEPDKAIGLTPHSDATGLTILLQ-VNEVEGLQI 252

Query: 254 LKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIVTFYAPSYDI 313
            KD KWV V+PLPNALV+N+GD +E++TNG Y+S+EHR V + + +RLS+ +F+   +  
Sbjct: 253 KKDGKWVSVKPLPNALVVNVGDILEIITNGTYRSIEHRGVVNSEKERLSVASFHNTGFGK 312

Query: 314 ELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKI 354
           E+GPM   V+++    ++  +  EY     + +L GK  L+  +I
Sbjct: 313 EIGPMRSLVERHKGALFKTLTTEEYFHGLFSRELDGKAYLDVMRI 353

BLAST of Csor.00g252240 vs. TAIR 10
Match: AT4G25300.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 279.3 bits (713), Expect = 4.5e-75
Identity = 144/347 (41.50%), Postives = 215/347 (61.96%), Query Frame = 0

Query: 14  IDDVQELRKFKP-NTIPERFIRGVQERPTPTALISS--SDLPTIDLSKLLKGNR--DEIL 73
           +  VQE+ K K   T+P R++R  Q+     A+ S   + +P ID+S L        EI 
Sbjct: 13  VPSVQEMVKEKMITTVPPRYVRSDQD-VAEIAVDSGLRNQIPIIDMSLLCSSTSMDSEID 72

Query: 74  QLASACEEWGFFQVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGTVQGYGQAFV 133
           +L SAC+EWGFFQ++NHG+  + L  ++ E  +FF LP+EEK+     P  ++G+GQ FV
Sbjct: 73  KLDSACKEWGFFQLVNHGMESSFLNKVKSEVQDFFNLPMEEKKNLWQQPDEIEGFGQVFV 132

Query: 134 FSEHQKLDWCNMFALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQNLLKYIALG 193
            SE QKLDW +MF L + P  +R P L+PK P  F +T+++YS E + + + LL  IA+ 
Sbjct: 133 VSEEQKLDWADMFFLTMQPVRLRKPHLFPKLPLPFRDTLDMYSAEVKSIAKILLGKIAVA 192

Query: 194 LGLKEDVFEKAFGVAV-QAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGKGNSV-GL 253
           L +K +  +K F   + Q IR+NYYP C  PD V+GL+PHSD + LT+L Q   N V GL
Sbjct: 193 LKIKPEEMDKLFDDELGQRIRLNYYPRCPEPDKVIGLTPHSDSTGLTILLQ--ANEVEGL 252

Query: 254 QILKDDKWVPVQPLPNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIVTFYAPSY 313
           QI K+ KWV V+PLPNALV+N+GD +E++TNG Y+S+EHR V + + +RLS+  F+    
Sbjct: 253 QIKKNAKWVSVKPLPNALVVNVGDILEIITNGTYRSIEHRGVVNSEKERLSVAAFHNIGL 312

Query: 314 DIELGPMPEFVDKNNPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKI 354
             E+GPM   V+++    ++  +  EY     + +L GK  L+  ++
Sbjct: 313 GKEIGPMRSLVERHKAAFFKSVTTEEYFNGLFSRELDGKAYLDVMRL 356

BLAST of Csor.00g252240 vs. TAIR 10
Match: AT1G78550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 277.3 bits (708), Expect = 1.7e-74
Identity = 139/333 (41.74%), Postives = 206/333 (61.86%), Query Frame = 0

Query: 27  TIPERFIRGVQERPTPTALIS----SSDLPTIDLSKL--LKGNRDEILQLASACEEWGFF 86
           TIP R++R  QE+   T +++    SS++P ID+++L  +     E+ +L  AC++WGFF
Sbjct: 28  TIPPRYVRVDQEK---TEILNDSSLSSEIPVIDMTRLCSVSAMDSELKKLDFACQDWGFF 87

Query: 87  QVINHGIALNLLESIEGEAMEFFGLPLEEKQKYRMAPGTVQGYGQAFVFSEHQKLDWCNM 146
           Q++NHGI  + LE +E E  EFF LP++EKQK     G  +G+GQ  + SE+QKLDW +M
Sbjct: 88  QLVNHGIDSSFLEKLETEVQEFFNLPMKEKQKLWQRSGEFEGFGQVNIVSENQKLDWGDM 147

Query: 147 FALGVIPDSIRNPLLWPKKPTNFSNTVEIYSKEARKLCQNLLKYIALGLGLKEDVFEKAF 206
           F L   P   R   L+ K P  F  T+E YS E + + + L   +A  L +K +  E  F
Sbjct: 148 FILTTEPIRSRKSHLFSKLPPPFRETLETYSSEVKSIAKILFAKMASVLEIKHEEMEDLF 207

Query: 207 GVAVQAIRMNYYPPCSRPDLVLGLSPHSDGSALTVLQQGKGNSVGLQILKDDKWVPVQPL 266
               Q+I++NYYPPC +PD V+GL+ HSD + LT+L Q      GLQI KD KWV V+PL
Sbjct: 208 DDVWQSIKINYYPPCPQPDQVMGLTQHSDAAGLTILLQ-VNQVEGLQIKKDGKWVVVKPL 267

Query: 267 PNALVINIGDTMEVVTNGRYKSVEHRAVTHKQTDRLSIVTFYAPSYDIELGPMPEFVDKN 326
            +ALV+N+G+ +E++TNGRY+S+EHRAV + + +RLS+  F++P  +  + P    VD+ 
Sbjct: 268 RDALVVNVGEILEIITNGRYRSIEHRAVVNSEKERLSVAMFHSPGKETIIRPAKSLVDRQ 327

Query: 327 NPCKYRRYSHGEYSKHYVTNKLQGKKTLEFAKI 354
             C ++  S  EY   + T KL GK  L+  +I
Sbjct: 328 KQCLFKSMSTQEYFDAFFTQKLNGKSHLDLMRI 356

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q392242.5e-7842.20Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1[more]
D4N5012.9e-7140.06Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=34... [more]
Q94LP41.1e-7038.732-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947... [more]
O804492.5e-7039.38Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1[more]
D4N5021.6e-6939.77Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6597511.17.79e-267100.00Protein SRG1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022936789.14.51e-26699.72protein SRG1-like [Cucurbita moschata] >KAG7028968.1 Protein SRG1 [Cucurbita arg... [more]
XP_022973772.13.16e-26499.44protein SRG1-like [Cucurbita maxima][more]
XP_023539447.18.68e-26298.61protein SRG1-like [Cucurbita pepo subsp. pepo][more]
XP_038905272.11.85e-24792.20protein SRG1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1F9A32.18e-26699.72protein SRG1-like OS=Cucurbita moschata OX=3662 GN=LOC111443268 PE=3 SV=1[more]
A0A6J1I8G01.53e-26499.44protein SRG1-like OS=Cucurbita maxima OX=3661 GN=LOC111472333 PE=3 SV=1[more]
A0A5D3CXG45.78e-24490.81Protein SRG1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G00820 P... [more]
A0A6J1IXQ51.66e-24391.09protein SRG1-like OS=Cucurbita maxima OX=3661 GN=LOC111479417 PE=3 SV=1[more]
A0A6J1GW691.93e-24291.09protein SRG1-like OS=Cucurbita moschata OX=3662 GN=LOC111457443 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G21420.12.0e-14468.232-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G17020.11.8e-7942.20senescence-related gene 1 [more]
AT4G25310.14.8e-7742.032-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G25300.14.5e-7541.502-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G78550.11.7e-7441.742-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 205..304
e-value: 7.8E-25
score: 87.3
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 51..158
e-value: 8.5E-27
score: 94.3
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 12..345
e-value: 7.0E-106
score: 356.3
NoneNo IPR availablePANTHERPTHR47991OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASEcoord: 10..355
NoneNo IPR availablePANTHERPTHR47991:SF9PROTEIN SRG1coord: 10..355
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 16..345
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 202..304
score: 13.08917

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g252240.m01Csor.00g252240.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1901601 strigolactone biosynthetic process
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding