Csor.00g251940 (gene) Silver-seed gourd (wild; sororia) v1

BLAST of Csor.00g251940 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 553.9 bits (1426), Expect = 2.1e-156
Identity = 269/536 (50.19%), Postives = 373/536 (69.59%), Query Frame = 0

Query: 28  NGVSGGGEDPGSSNAAIKTLCEPTTYRETCEEALVKSNVDSKDPRELIKAGFHFAIEQLK 87
           +G S G  +  +S  A+K +C PT YR+TCE+ L+K+  ++ DP EL+K  F+  ++Q+ 
Sbjct: 42  DGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQIT 101

Query: 88  VALQNSPTLRAAAADPMAQKAVDACDELMDYAIDDLLITFDKMANSFSLHQINNYLGNIR 147
            A + S T+     D   + A+D C ELMDYA+D+L  +F+++   F  H ++  L N+R
Sbjct: 102 DAAKKSQTIMELQKDSRTRMALDQCKELMDYALDELSNSFEEL-GKFEFHLLDEALINLR 161

Query: 148 IWLSGALTYQETCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEITSFVSSLWQAL 207
           IWLS A++++ETCL+GF+   G+ GE MK  LKT+ E+T NGL ++ E+++FV  + Q  
Sbjct: 162 IWLSAAISHEETCLEGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQM-QIP 221

Query: 208 GLSKGRQLRIDESSEEFQDEEPSWVNDK-RGLLEATGA--NIKADVVVAKDGSGKYTTIT 267
           GL+  R L     +E F    PSWV+ + R LL+A  A  ++K D+VVA+DGSG+Y TI 
Sbjct: 222 GLNSRRLL-----AEGF----PSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTIN 281

Query: 268 EALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTMTYVMMIGDGPTKTKITASANVIDGTPT 327
           EALQ VPKK NTTFV+++K G+Y+E V V+KTM++++ IGDGP KT I+ + N  DG  T
Sbjct: 282 EALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITT 341

Query: 328 FKSATFAAVGTNFIGKDLWFENSAGPEKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAH 387
           +++AT A VG  FI K++ FEN+AG  KHQAVA+RVQSD SIF+NCR DGYQDTLYTH+H
Sbjct: 342 YRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSH 401

Query: 388 RQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRKPMKGQTCLVTAQGRTQRKEPTGIVLQN 447
           RQFFRDCTI+GT+DF+FG+AA V QNC + VRKP+  Q C +TA GR   +E TG V Q 
Sbjct: 402 RQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQG 461

Query: 448 CQISSDPDYYPVRHTIKSFLGRPWKLYSRTVVMQSQIDDLIQPEGWLAWEGTFALKTLFY 507
           C I+ +PDY  V+ T K++LGRPWK YSRT++M + I D +QP+GW  W G F LKTLFY
Sbjct: 462 CTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFY 521

Query: 508 AEFDNRGPGAATQNRVTWRGIKQITADHATKFAPGLFIGGNQWVKPTGIPYSGGLM 561
           +E  N GPG+A  NRVTW GIK ++ +   KF P  +I G+ W+   G+PY+ GL+
Sbjct: 522 SEVQNTGPGSALANRVTWAGIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

BLAST of Csor.00g251940 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 4.5e-151
Identity = 263/564 (46.63%), Postives = 375/564 (66.49%), Query Frame = 0

Query: 9   KNNKVALVGLLSLFLVALVNGV----------SGGGEDPGSSNAAIKTLCEPTTYRETCE 68
           +  +  ++ + S+ L+++V  V          + G E+  +S  AIK +C PT Y+ETCE
Sbjct: 12  RKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCE 71

Query: 69  EALVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPTLRAAAADPMAQKAVDACDELMDY 128
           + L K   D+ DP EL+K  F+  ++Q+    + S T+     DP A+ A+D C ELMDY
Sbjct: 72  DTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMALDQCKELMDY 131

Query: 129 AIDDLLITFDKMANSFSLHQINNYLGNIRIWLSGALTYQETCLDGFENVPGDTGEKMKNL 188
           AI +L  +F+++   F  H+++  L  +RIWLS  +++++TCLDGF+   G+ GE +K  
Sbjct: 132 AIGELSKSFEEL-GKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKA 191

Query: 189 LKTSKEMTANGLVMVGEITSFVSSLWQALGLSKGRQLRIDESSEEFQDEEPSWVNDK-RG 248
           LKT+ ++T NGL MV E+++++  + Q   ++  R L     S+EF    PSW++ + R 
Sbjct: 192 LKTAVQLTHNGLAMVTEMSNYLGQM-QIPEMNSRRLL-----SQEF----PSWMDARARR 251

Query: 249 LLEATGANIKADVVVAKDGSGKYTTITEALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTM 308
           LL A  + +K D+VVA+DGSG+Y TI EAL  VPKK NTTFV+++KEG+Y+E V V+++M
Sbjct: 252 LLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSM 311

Query: 309 TYVMMIGDGPTKTKITASANVIDGTPTFKSATFAAVGTNFIGKDLWFENSAGPEKHQAVA 368
           T+++ IGDGP KT I+ S +  DG  T+K+AT A VG +FI K++ FEN+AG  KHQAVA
Sbjct: 312 THLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVA 371

Query: 369 LRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRK 428
           +RV +D SIFYNC+ DGYQDTLY H+HRQF+RDCTI+GT+DF+FG+AA V QNC + VRK
Sbjct: 372 IRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRK 431

Query: 429 PMKGQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDYYPVRHTIKSFLGRPWKLYSRTVVM 488
           P+  Q C +TA GR   +E TG VLQ C I  +PDY  V+   K++LGRPWK YSRT++M
Sbjct: 432 PLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIM 491

Query: 489 QSQIDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRGIKQITADHATKFA 548
            + I D + PEGW  W G F L TLFY+E  N GPGAA   RVTW GIK+++ +   KF 
Sbjct: 492 NTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFT 551

Query: 549 PGLFIGGNQWVKPTGIPYSGGLMT 562
           P  +I G+ W+   G+PY  GL +
Sbjct: 552 PAQYIQGDAWIPGKGVPYILGLFS 564

BLAST of Csor.00g251940 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 6.1e-148
Identity = 259/524 (49.43%), Postives = 360/524 (68.70%), Query Frame = 0

Query: 39  SSNAAIKTLCEPTTYRETCEEALVKSNVDSKDPRELIKAGFHFAIEQLKVALQN-SPTLR 98
           ++  A++ +C PT Y+ETC  +L+K++ DS  P +LIK GF+  I  ++ +++  S  L 
Sbjct: 50  TATTAVEAVCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELT 109

Query: 99  AAAA-DPMAQKAVDACDELMDYAIDDLLITFDKMANSFSLHQINNYLGNIRIWLSGALTY 158
           A AA D   + A++ C++LM+ A DDL    D   + FS+ QI +++ ++R+WLSG++ Y
Sbjct: 110 AKAANDKDTKGALELCEKLMNDATDDLKKCLDNF-DGFSIPQIEDFVEDLRVWLSGSIAY 169

Query: 159 QETCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEITSFVSSLWQALGLS--KGRQ 218
           Q+TC+D FE       + M+ + KTS+E+T+NGL M+  I++ +   +   G++   G+ 
Sbjct: 170 QQTCMDTFEETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGE-FNVTGVTGDLGKY 229

Query: 219 LRIDESSEEFQDEEPSWVNDKRGLLEATGANIKADVVVAKDGSGKYTTITEALQEVPKKS 278
            R   S+E   D  PSWV      L AT   +KA+VVVA DGSG+Y TI EAL  VPK +
Sbjct: 230 ARKLLSAE---DGIPSWVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPKAN 289

Query: 279 NTTFVIYVKEGVYEEQVMVDKTMTYVMMIGDGPTKTKITASANVIDG-TPTFKSATFAAV 338
              FVIY+K+GVY E+V V K MT+V  IGDGPTKTKIT S N   G   T+ +AT A  
Sbjct: 290 QKPFVIYIKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAIN 349

Query: 339 GTNFIGKDLWFENSAGPEKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTI 398
           G NF  K++ FEN+AGPE HQAVALRV +D+++FYNC++DGYQDTLY H+HRQFFRDCT+
Sbjct: 350 GDNFTAKNIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTV 409

Query: 399 TGTVDFIFGNAAVVLQNCKIFVRKPMKGQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDY 458
           +GTVDFIFG+  VVLQNC I VRKPMK Q+C++TAQGR+ ++E TG+VLQNC I+ +P Y
Sbjct: 410 SGTVDFIFGDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAY 469

Query: 459 YPVRHTIKSFLGRPWKLYSRTVVMQSQIDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPG 518
            PV+   K++LGRPWK +SRT++M + IDD+I P GWL W G FAL TL+YAE++N GPG
Sbjct: 470 IPVKSINKAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPG 529

Query: 519 AATQNRVTWRGIKQITADHATKFAPGLFIGGNQWVKPTGIPYSG 558
           +    RV W GIK+++   A +F P  F+ GN W+ P  +PY G
Sbjct: 530 SNQAQRVKWPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPYMG 568

BLAST of Csor.00g251940 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 477.2 bits (1227), Expect = 2.5e-133
Identity = 250/572 (43.71%), Postives = 365/572 (63.81%), Query Frame = 0

Query: 1   MASDGCGCKNNKVALVGLLSLFLVALVNGVS------GGGEDPG---SSNAAIKTLCEPT 60
           M SD  G K  K  + G +S FLV +V  V+         +D      +  A++ +C PT
Sbjct: 1   MGSD--GDKKKKFIVAGSVSGFLVIMVVSVAVVTSKHSPKDDENHIRKTTKAVQAVCAPT 60

Query: 61  TYRETCEEALVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPTLRAAAAD--PMAQKAV 120
            +++TC  +L+ ++ DS DP +LIK GF   I+ +  +L+ +     A AD  P A+ A 
Sbjct: 61  DFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKGAF 120

Query: 121 DACDELMDYAIDDLLITFDKMANSFSLHQINNYLGNIRIWLSGALTYQETCLDGFENVPG 180
           + C++LM  AIDDL    D   + FS+ QI  ++ ++R+WLSG++ +Q+TC+D F  +  
Sbjct: 121 ELCEKLMIDAIDDLKKCMD---HGFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIKS 180

Query: 181 DTGEKMKNLLKTSKEMTANGLVMVGEITSFVSSLWQALGLSKGRQLRIDESSEEFQDEEP 240
           +  + M  + KTS+E+++N L MV  I++ + +     GL+ G   +        +D  P
Sbjct: 181 NLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNS-NLTGLT-GALAKYARKLLSTEDSIP 240

Query: 241 SWVN-DKRGLLEATGAN---IKADVVVAKDGSGKYTTITEALQEVPKKSNTTFVIYVKEG 300
           +WV  + R L+ A G     +KA+ VVA+DG+G++ TIT+AL  VPK +   F+I++KEG
Sbjct: 241 TWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFIIHIKEG 300

Query: 301 VYEEQVMVDKTMTYVMMIGDGPTKTKITASANV-IDGTPTFKSATFAAVGTNFIGKDLWF 360
           +Y+E+V V K M +V  IGDGP KT IT S N  I    TF +AT    G +F  K++  
Sbjct: 301 IYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNIGI 360

Query: 361 ENSAGPEKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNA 420
           EN+AGPE  QAVALRV +D ++F++C++DG+QDTLY H+HRQF+RDCT++GTVDFIFG+A
Sbjct: 361 ENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFGDA 420

Query: 421 AVVLQNCKIFVRKPMKGQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDYYPVRHTIKSFL 480
             +LQNCKI VRKP KGQTC+VTAQGR+  +E TG+VL  C I+ DP Y P++   K++L
Sbjct: 421 KCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKAYL 480

Query: 481 GRPWKLYSRTVVMQSQIDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRG 540
           GRPWK +SRT++M++ IDD+I P GWL W G FALKTL+YAE  N GPG+    RV W G
Sbjct: 481 GRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKWPG 540

Query: 541 IKQITADHATKFAPGLFIGGNQWVKPTGIPYS 557
           IK++T   A  +    F+ G+ W+  T +PY+
Sbjct: 541 IKKLTPQDALLYTGDRFLRGDTWIPQTQVPYT 565

BLAST of Csor.00g251940 vs. ExPASy Swiss-Prot
Match: O23447 (Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana OX=3702 GN=PME43 PE=2 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.7e-118
Identity = 249/701 (35.52%), Postives = 361/701 (51.50%), Query Frame = 0

Query: 11  NKVALVGLLSLFLVALVNGVSGGGEDPGS-------------SNAAIKTLCEPTTYRETC 70
           NK  L+G+ +L +  ++   +  G  P               +   +  +C  T Y++ C
Sbjct: 2   NKYVLLGVTALIMAMVICVEANDGNSPSRKMEEVHRESKLMITKTTVSIICASTDYKQDC 61

Query: 71  EEALVKSNVDSKDPRELIKAGFHFAIEQLKVALQNS--PTLRAAAADPMAQKAVDACDEL 130
             +L  + V S DPR LI++ F  AI  ++  +          A AD   ++A++ C EL
Sbjct: 62  TTSL--ATVRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTCREL 121

Query: 131 MDYAIDDLLITFDKMANSFSLHQINNYLGNIRIWLSGALTYQETCLDGFENVPGDTGEKM 190
           MD AIDDL  T DK    F   ++++++ ++ +WLSG++TYQ+TC+DGFE +  +    M
Sbjct: 122 MDDAIDDLRKTRDKF-RGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAVMM 181

Query: 191 KNLLKTSKEMTANGLVMVGEITSFV----------------------------------- 250
           + +++  + +T+NGL +   +   +                                   
Sbjct: 182 ERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSSQD 241

Query: 251 ---------------------------------SSLWQALGLSKGRQLR----------- 310
                                            SS  + L  SK +Q+            
Sbjct: 242 SPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKSAF 301

Query: 311 -----IDESSEEF----------------------------------------------- 370
                +D+SS++                                                
Sbjct: 302 SGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSLKD 361

Query: 371 -----QDEEPSWVN-DKRGLLEATGAN--IKADVVVAKDGSGKYTTITEALQEVPKKSNT 430
                + E P WV    R LL     N  IKA+VVVAKDGSGK  TI +AL  VP K+  
Sbjct: 362 RHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVPMKNTK 421

Query: 431 TFVIYVKEGVYEEQVMVDKTMTYVMMIGDGPTKTKITAS-ANVIDGTPTFKSATFAAVGT 490
            FVI++KEGVY+E+V V K M +VM +GDGPTKT IT   A + D   T+++A+ A  G 
Sbjct: 422 KFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAVNGD 481

Query: 491 NFIGKDLWFENSAGPEKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITG 550
            F+ KD+ FEN+AG  +HQAVALRV +D ++F+NC M+GYQDTLY H HRQF+R+C ++G
Sbjct: 482 YFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCRVSG 541

Query: 551 TVDFIFGNAAVVLQNCKIFVRKPMKGQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDYYP 557
           T+DF+FG+A  V QNC+  +R+PM+ Q C+VTAQGR  R+E TGIV+ N +I+ D  Y P
Sbjct: 542 TIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDASYLP 601

BLAST of Csor.00g251940 vs. NCBI nr
Match: KAG6597541.1 (putative pectinesterase/pectinesterase inhibitor 21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1135 bits (2937), Expect = 0.0
Identity = 562/562 (100.00%), Postives = 562/562 (100.00%), Query Frame = 0

Query: 1   MASDGCGCKNNKVALVGLLSLFLVALVNGVSGGGEDPGSSNAAIKTLCEPTTYRETCEEA 60
           MASDGCGCKNNKVALVGLLSLFLVALVNGVSGGGEDPGSSNAAIKTLCEPTTYRETCEEA
Sbjct: 1   MASDGCGCKNNKVALVGLLSLFLVALVNGVSGGGEDPGSSNAAIKTLCEPTTYRETCEEA 60

Query: 61  LVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPTLRAAAADPMAQKAVDACDELMDYAI 120
           LVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPTLRAAAADPMAQKAVDACDELMDYAI
Sbjct: 61  LVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPTLRAAAADPMAQKAVDACDELMDYAI 120

Query: 121 DDLLITFDKMANSFSLHQINNYLGNIRIWLSGALTYQETCLDGFENVPGDTGEKMKNLLK 180
           DDLLITFDKMANSFSLHQINNYLGNIRIWLSGALTYQETCLDGFENVPGDTGEKMKNLLK
Sbjct: 121 DDLLITFDKMANSFSLHQINNYLGNIRIWLSGALTYQETCLDGFENVPGDTGEKMKNLLK 180

Query: 181 TSKEMTANGLVMVGEITSFVSSLWQALGLSKGRQLRIDESSEEFQDEEPSWVNDKRGLLE 240
           TSKEMTANGLVMVGEITSFVSSLWQALGLSKGRQLRIDESSEEFQDEEPSWVNDKRGLLE
Sbjct: 181 TSKEMTANGLVMVGEITSFVSSLWQALGLSKGRQLRIDESSEEFQDEEPSWVNDKRGLLE 240

Query: 241 ATGANIKADVVVAKDGSGKYTTITEALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTMTYV 300
           ATGANIKADVVVAKDGSGKYTTITEALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTMTYV
Sbjct: 241 ATGANIKADVVVAKDGSGKYTTITEALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTMTYV 300

Query: 301 MMIGDGPTKTKITASANVIDGTPTFKSATFAAVGTNFIGKDLWFENSAGPEKHQAVALRV 360
           MMIGDGPTKTKITASANVIDGTPTFKSATFAAVGTNFIGKDLWFENSAGPEKHQAVALRV
Sbjct: 301 MMIGDGPTKTKITASANVIDGTPTFKSATFAAVGTNFIGKDLWFENSAGPEKHQAVALRV 360

Query: 361 QSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRKPMK 420
           QSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRKPMK
Sbjct: 361 QSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRKPMK 420

Query: 421 GQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDYYPVRHTIKSFLGRPWKLYSRTVVMQSQ 480
           GQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDYYPVRHTIKSFLGRPWKLYSRTVVMQSQ
Sbjct: 421 GQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDYYPVRHTIKSFLGRPWKLYSRTVVMQSQ 480

Query: 481 IDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRGIKQITADHATKFAPGL 540
           IDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRGIKQITADHATKFAPGL
Sbjct: 481 IDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRGIKQITADHATKFAPGL 540

Query: 541 FIGGNQWVKPTGIPYSGGLMTI 562
           FIGGNQWVKPTGIPYSGGLMTI
Sbjct: 541 FIGGNQWVKPTGIPYSGGLMTI 562

BLAST of Csor.00g251940 vs. NCBI nr
Match: XP_022949434.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 1134 bits (2932), Expect = 0.0
Identity = 561/562 (99.82%), Postives = 561/562 (99.82%), Query Frame = 0

Query: 1   MASDGCGCKNNKVALVGLLSLFLVALVNGVSGGGEDPGSSNAAIKTLCEPTTYRETCEEA 60
           MASDGCGCKNNKVALVGLLSLFLVALVNGVSGGGEDPGSSNAAIKTLCEPTTYRETCEEA
Sbjct: 1   MASDGCGCKNNKVALVGLLSLFLVALVNGVSGGGEDPGSSNAAIKTLCEPTTYRETCEEA 60

Query: 61  LVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPTLRAAAADPMAQKAVDACDELMDYAI 120
           LVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPTLRAAAADPMAQKAVDACDELMDYAI
Sbjct: 61  LVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPTLRAAAADPMAQKAVDACDELMDYAI 120

Query: 121 DDLLITFDKMANSFSLHQINNYLGNIRIWLSGALTYQETCLDGFENVPGDTGEKMKNLLK 180
           DDLLITFDKMANSFSLHQINNYLGNIRIWLSGALTYQETCLDGFENVPGDTGEKMKNLLK
Sbjct: 121 DDLLITFDKMANSFSLHQINNYLGNIRIWLSGALTYQETCLDGFENVPGDTGEKMKNLLK 180

Query: 181 TSKEMTANGLVMVGEITSFVSSLWQALGLSKGRQLRIDESSEEFQDEEPSWVNDKRGLLE 240
           TSKEMTANGLVMVGEITSFVSSLWQALGLSKGRQLR DESSEEFQDEEPSWVNDKRGLLE
Sbjct: 181 TSKEMTANGLVMVGEITSFVSSLWQALGLSKGRQLRTDESSEEFQDEEPSWVNDKRGLLE 240

Query: 241 ATGANIKADVVVAKDGSGKYTTITEALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTMTYV 300
           ATGANIKADVVVAKDGSGKYTTITEALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTMTYV
Sbjct: 241 ATGANIKADVVVAKDGSGKYTTITEALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTMTYV 300

Query: 301 MMIGDGPTKTKITASANVIDGTPTFKSATFAAVGTNFIGKDLWFENSAGPEKHQAVALRV 360
           MMIGDGPTKTKITASANVIDGTPTFKSATFAAVGTNFIGKDLWFENSAGPEKHQAVALRV
Sbjct: 301 MMIGDGPTKTKITASANVIDGTPTFKSATFAAVGTNFIGKDLWFENSAGPEKHQAVALRV 360

Query: 361 QSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRKPMK 420
           QSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRKPMK
Sbjct: 361 QSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRKPMK 420

Query: 421 GQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDYYPVRHTIKSFLGRPWKLYSRTVVMQSQ 480
           GQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDYYPVRHTIKSFLGRPWKLYSRTVVMQSQ
Sbjct: 421 GQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDYYPVRHTIKSFLGRPWKLYSRTVVMQSQ 480

Query: 481 IDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRGIKQITADHATKFAPGL 540
           IDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRGIKQITADHATKFAPGL
Sbjct: 481 IDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRGIKQITADHATKFAPGL 540

Query: 541 FIGGNQWVKPTGIPYSGGLMTI 562
           FIGGNQWVKPTGIPYSGGLMTI
Sbjct: 541 FIGGNQWVKPTGIPYSGGLMTI 562

BLAST of Csor.00g251940 vs. NCBI nr
Match: XP_023540666.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1093 bits (2827), Expect = 0.0
Identity = 541/562 (96.26%), Postives = 549/562 (97.69%), Query Frame = 0

Query: 1   MASDGCGCKNNKVALVGLLSLFLVALVNGVSGGGEDPGSSNAAIKTLCEPTTYRETCEEA 60
           MA D  GCKNNKVALVGLLSLFLVALVNGVSG GED GSSNA IKTLCEPTTYRETCEEA
Sbjct: 1   MAYDSGGCKNNKVALVGLLSLFLVALVNGVSGAGEDTGSSNADIKTLCEPTTYRETCEEA 60

Query: 61  LVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPTLRAAAADPMAQKAVDACDELMDYAI 120
           LVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPT+RAAAADPMAQKAVDACDELMDYAI
Sbjct: 61  LVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPTVRAAAADPMAQKAVDACDELMDYAI 120

Query: 121 DDLLITFDKMANSFSLHQINNYLGNIRIWLSGALTYQETCLDGFENVPGDTGEKMKNLLK 180
           DDLL+TFDKMANSFSLHQINNYLGNIRIWLSGALTYQETCLDGFENVPGDTGEKMKNLLK
Sbjct: 121 DDLLVTFDKMANSFSLHQINNYLGNIRIWLSGALTYQETCLDGFENVPGDTGEKMKNLLK 180

Query: 181 TSKEMTANGLVMVGEITSFVSSLWQALGLSKGRQLRIDESSEEFQDEEPSWVNDKRGLLE 240
           TSKEMTANGLVMVGEITS VSSLWQALGLSKGRQLR DESSEEFQD+EPSWV+++RGLLE
Sbjct: 181 TSKEMTANGLVMVGEITSLVSSLWQALGLSKGRQLRTDESSEEFQDDEPSWVHERRGLLE 240

Query: 241 ATGANIKADVVVAKDGSGKYTTITEALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTMTYV 300
           ATGANIKADVVVAKDGSGKYTTITEALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTMTYV
Sbjct: 241 ATGANIKADVVVAKDGSGKYTTITEALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTMTYV 300

Query: 301 MMIGDGPTKTKITASANVIDGTPTFKSATFAAVGTNFIGKDLWFENSAGPEKHQAVALRV 360
           MMIGDGPTKTKITAS NVIDGTPTFKSATFAAVGTNFIGKDLWFENSAGPEKHQAVALRV
Sbjct: 301 MMIGDGPTKTKITASKNVIDGTPTFKSATFAAVGTNFIGKDLWFENSAGPEKHQAVALRV 360

Query: 361 QSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRKPMK 420
           QSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRKPMK
Sbjct: 361 QSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRKPMK 420

Query: 421 GQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDYYPVRHTIKSFLGRPWKLYSRTVVMQSQ 480
           GQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDYYPVRHTIKSFLGRPWKLYSRTVVMQ Q
Sbjct: 421 GQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDYYPVRHTIKSFLGRPWKLYSRTVVMQCQ 480

Query: 481 IDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRGIKQITADHATKFAPGL 540
           IDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRGIKQITADHA K+AP L
Sbjct: 481 IDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRGIKQITADHAVKYAPAL 540

Query: 541 FIGGNQWVKPTGIPYSGGLMTI 562
           FIGGN+WVK TGIPYSGGLMTI
Sbjct: 541 FIGGNRWVKQTGIPYSGGLMTI 562

BLAST of Csor.00g251940 vs. NCBI nr
Match: XP_022973683.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 1070 bits (2767), Expect = 0.0
Identity = 531/562 (94.48%), Postives = 541/562 (96.26%), Query Frame = 0

Query: 1   MASDGCGCKNNKVALVGLLSLFLVALVNGVSGGGEDPGSSNAAIKTLCEPTTYRETCEEA 60
           MA DG     NK ALVGLLSLFLVALVNGV+G GED GSSNAAIKTLCEPTTYRETCEEA
Sbjct: 1   MAYDG-----NKAALVGLLSLFLVALVNGVNGAGEDHGSSNAAIKTLCEPTTYRETCEEA 60

Query: 61  LVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPTLRAAAADPMAQKAVDACDELMDYAI 120
           LVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPT+RAAA DPMAQKAVD CDELMDYAI
Sbjct: 61  LVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPTVRAAATDPMAQKAVDTCDELMDYAI 120

Query: 121 DDLLITFDKMANSFSLHQINNYLGNIRIWLSGALTYQETCLDGFENVPGDTGEKMKNLLK 180
           DDLL+TFDKMANSFS+HQINNYLGNIRIWLSGALTYQETCLDGFENV GDTGEKMKNLLK
Sbjct: 121 DDLLVTFDKMANSFSVHQINNYLGNIRIWLSGALTYQETCLDGFENVSGDTGEKMKNLLK 180

Query: 181 TSKEMTANGLVMVGEITSFVSSLWQALGLSKGRQLRIDESSEEFQDEEPSWVNDKRGLLE 240
           TSKEMTANGLVMVGEITS VSSLWQALGLSKGRQL+ +ESSEEFQDEEPSWVND+RGLLE
Sbjct: 181 TSKEMTANGLVMVGEITSLVSSLWQALGLSKGRQLQTEESSEEFQDEEPSWVNDRRGLLE 240

Query: 241 ATGANIKADVVVAKDGSGKYTTITEALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTMTYV 300
           ATGANIKADVVVAKDGSGKY TITEALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTMTYV
Sbjct: 241 ATGANIKADVVVAKDGSGKYKTITEALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTMTYV 300

Query: 301 MMIGDGPTKTKITASANVIDGTPTFKSATFAAVGTNFIGKDLWFENSAGPEKHQAVALRV 360
           MMIGDGPTKTKITAS NV+DGTPTFKSATFAAVGTNFIGKDLWFENSAGPEKHQAVALRV
Sbjct: 301 MMIGDGPTKTKITASKNVVDGTPTFKSATFAAVGTNFIGKDLWFENSAGPEKHQAVALRV 360

Query: 361 QSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRKPMK 420
           QSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRKPMK
Sbjct: 361 QSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRKPMK 420

Query: 421 GQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDYYPVRHTIKSFLGRPWKLYSRTVVMQSQ 480
           GQTCLVTAQGRTQRKEPTGI+LQNCQISSDPDYYPVRHTIKSFLGRPWK YSRTVVMQ Q
Sbjct: 421 GQTCLVTAQGRTQRKEPTGIILQNCQISSDPDYYPVRHTIKSFLGRPWKQYSRTVVMQCQ 480

Query: 481 IDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRGIKQITADHATKFAPGL 540
           IDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRGIKQITADHA KFAP L
Sbjct: 481 IDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRGIKQITADHAVKFAPAL 540

Query: 541 FIGGNQWVKPTGIPYSGGLMTI 562
           FIGGN+WVK TGIPYSGGLMTI
Sbjct: 541 FIGGNRWVKQTGIPYSGGLMTI 557

BLAST of Csor.00g251940 vs. NCBI nr
Match: XP_022973798.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 949 bits (2453), Expect = 0.0
Identity = 460/563 (81.71%), Postives = 505/563 (89.70%), Query Frame = 0

Query: 1   MASDGCGCKNNKVALVGLLSLFLVALVNGVSGGGEDPGSSNAAIKTLCEPTTYRETCEEA 60
           MA DG GCKNNKVALVGLLSLFLVALVNG SG GED GSSN AIKT+CEPTTYRETCEEA
Sbjct: 1   MAYDGGGCKNNKVALVGLLSLFLVALVNGASGAGEDSGSSNVAIKTICEPTTYRETCEEA 60

Query: 61  LVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPTLRAAAADPMAQKAVDACDELMDYAI 120
           LVKSNVDSKDPRELIKAGFHFAIEQLK A+ NSPTL+ AA DPMA++A+D CDEL+DYAI
Sbjct: 61  LVKSNVDSKDPRELIKAGFHFAIEQLKAAIANSPTLKQAATDPMAKEAIDTCDELIDYAI 120

Query: 121 DDLLITFDKMANSFSLHQINNYLGNIRIWLSGALTYQETCLDGFENVPGDTGEKMKNLLK 180
           DDLL TFD++ +SFSL++++NYL N+RIWLSGALTYQETCLD FENV GDTGEKM NLLK
Sbjct: 121 DDLLTTFDQITDSFSLNRVDNYLKNLRIWLSGALTYQETCLDAFENVTGDTGEKMTNLLK 180

Query: 181 TSKEMTANGLVMVGEITSFVSSLWQALGLSK-GRQLRIDESSEEFQDEEPSWVNDKRGLL 240
           TSKEMTANGLVMVGE+TS VSSLW+ALGL + GRQLR  E  EE ++EEPSWVND+RGLL
Sbjct: 181 TSKEMTANGLVMVGEVTSLVSSLWKALGLPETGRQLRAKELDEEDEEEEPSWVNDRRGLL 240

Query: 241 EATGANIKADVVVAKDGSGKYTTITEALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTMTY 300
           +ATGA I+A+VVVAKDGSGKY TIT+ALQ+VP+KSN TFVIYVKEGVY+EQVMVDKTMT+
Sbjct: 241 QATGAKIRANVVVAKDGSGKYKTITQALQDVPRKSNKTFVIYVKEGVYQEQVMVDKTMTW 300

Query: 301 VMMIGDGPTKTKITASANVIDGTPTFKSATFAAVGTNFIGKDLWFENSAGPEKHQAVALR 360
           VMMIGDGPTKTKITA  N  DGT TFK+ATFAAVGTNFIGKDLWFENSAGPEKHQAVALR
Sbjct: 301 VMMIGDGPTKTKITAGKNFKDGTSTFKTATFAAVGTNFIGKDLWFENSAGPEKHQAVALR 360

Query: 361 VQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRKPM 420
           VQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGT+DFIFGNAAV+LQNCKIFVRKPM
Sbjct: 361 VQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTIDFIFGNAAVILQNCKIFVRKPM 420

Query: 421 KGQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDYYPVRHTIKSFLGRPWKLYSRTVVMQS 480
           + Q C+VTAQGR +RKEPTGIVLQNC ISSDP ++PVRH  K++LGRPWK YSRT++MQ 
Sbjct: 421 ENQKCIVTAQGRLERKEPTGIVLQNCAISSDPGFFPVRHVFKTYLGRPWKQYSRTIIMQC 480

Query: 481 QIDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRGIKQITADHATKFAPG 540
           QIDDLIQPEGWL WEG FALKTLFY EFDNRGPGAA QNRV WRGIKQIT  HA  F PG
Sbjct: 481 QIDDLIQPEGWLPWEGDFALKTLFYTEFDNRGPGAAKQNRVKWRGIKQITPKHAIDFTPG 540

Query: 541 LFIGGNQWVKPTGIPYSGGLMTI 562
           LFI GN W++ TG+PY  G+ TI
Sbjct: 541 LFIRGNPWIRRTGVPYYSGMATI 563

BLAST of Csor.00g251940 vs. ExPASy TrEMBL
Match: A0A6J1GCT1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111452779 PE=3 SV=1)

HSP 1 Score: 1134 bits (2932), Expect = 0.0
Identity = 561/562 (99.82%), Postives = 561/562 (99.82%), Query Frame = 0

Query: 1   MASDGCGCKNNKVALVGLLSLFLVALVNGVSGGGEDPGSSNAAIKTLCEPTTYRETCEEA 60
           MASDGCGCKNNKVALVGLLSLFLVALVNGVSGGGEDPGSSNAAIKTLCEPTTYRETCEEA
Sbjct: 1   MASDGCGCKNNKVALVGLLSLFLVALVNGVSGGGEDPGSSNAAIKTLCEPTTYRETCEEA 60

Query: 61  LVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPTLRAAAADPMAQKAVDACDELMDYAI 120
           LVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPTLRAAAADPMAQKAVDACDELMDYAI
Sbjct: 61  LVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPTLRAAAADPMAQKAVDACDELMDYAI 120

Query: 121 DDLLITFDKMANSFSLHQINNYLGNIRIWLSGALTYQETCLDGFENVPGDTGEKMKNLLK 180
           DDLLITFDKMANSFSLHQINNYLGNIRIWLSGALTYQETCLDGFENVPGDTGEKMKNLLK
Sbjct: 121 DDLLITFDKMANSFSLHQINNYLGNIRIWLSGALTYQETCLDGFENVPGDTGEKMKNLLK 180

Query: 181 TSKEMTANGLVMVGEITSFVSSLWQALGLSKGRQLRIDESSEEFQDEEPSWVNDKRGLLE 240
           TSKEMTANGLVMVGEITSFVSSLWQALGLSKGRQLR DESSEEFQDEEPSWVNDKRGLLE
Sbjct: 181 TSKEMTANGLVMVGEITSFVSSLWQALGLSKGRQLRTDESSEEFQDEEPSWVNDKRGLLE 240

Query: 241 ATGANIKADVVVAKDGSGKYTTITEALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTMTYV 300
           ATGANIKADVVVAKDGSGKYTTITEALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTMTYV
Sbjct: 241 ATGANIKADVVVAKDGSGKYTTITEALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTMTYV 300

Query: 301 MMIGDGPTKTKITASANVIDGTPTFKSATFAAVGTNFIGKDLWFENSAGPEKHQAVALRV 360
           MMIGDGPTKTKITASANVIDGTPTFKSATFAAVGTNFIGKDLWFENSAGPEKHQAVALRV
Sbjct: 301 MMIGDGPTKTKITASANVIDGTPTFKSATFAAVGTNFIGKDLWFENSAGPEKHQAVALRV 360

Query: 361 QSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRKPMK 420
           QSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRKPMK
Sbjct: 361 QSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRKPMK 420

Query: 421 GQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDYYPVRHTIKSFLGRPWKLYSRTVVMQSQ 480
           GQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDYYPVRHTIKSFLGRPWKLYSRTVVMQSQ
Sbjct: 421 GQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDYYPVRHTIKSFLGRPWKLYSRTVVMQSQ 480

Query: 481 IDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRGIKQITADHATKFAPGL 540
           IDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRGIKQITADHATKFAPGL
Sbjct: 481 IDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRGIKQITADHATKFAPGL 540

Query: 541 FIGGNQWVKPTGIPYSGGLMTI 562
           FIGGNQWVKPTGIPYSGGLMTI
Sbjct: 541 FIGGNQWVKPTGIPYSGGLMTI 562

BLAST of Csor.00g251940 vs. ExPASy TrEMBL
Match: A0A6J1IDW2 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472268 PE=3 SV=1)

HSP 1 Score: 1070 bits (2767), Expect = 0.0
Identity = 531/562 (94.48%), Postives = 541/562 (96.26%), Query Frame = 0

Query: 1   MASDGCGCKNNKVALVGLLSLFLVALVNGVSGGGEDPGSSNAAIKTLCEPTTYRETCEEA 60
           MA DG     NK ALVGLLSLFLVALVNGV+G GED GSSNAAIKTLCEPTTYRETCEEA
Sbjct: 1   MAYDG-----NKAALVGLLSLFLVALVNGVNGAGEDHGSSNAAIKTLCEPTTYRETCEEA 60

Query: 61  LVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPTLRAAAADPMAQKAVDACDELMDYAI 120
           LVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPT+RAAA DPMAQKAVD CDELMDYAI
Sbjct: 61  LVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPTVRAAATDPMAQKAVDTCDELMDYAI 120

Query: 121 DDLLITFDKMANSFSLHQINNYLGNIRIWLSGALTYQETCLDGFENVPGDTGEKMKNLLK 180
           DDLL+TFDKMANSFS+HQINNYLGNIRIWLSGALTYQETCLDGFENV GDTGEKMKNLLK
Sbjct: 121 DDLLVTFDKMANSFSVHQINNYLGNIRIWLSGALTYQETCLDGFENVSGDTGEKMKNLLK 180

Query: 181 TSKEMTANGLVMVGEITSFVSSLWQALGLSKGRQLRIDESSEEFQDEEPSWVNDKRGLLE 240
           TSKEMTANGLVMVGEITS VSSLWQALGLSKGRQL+ +ESSEEFQDEEPSWVND+RGLLE
Sbjct: 181 TSKEMTANGLVMVGEITSLVSSLWQALGLSKGRQLQTEESSEEFQDEEPSWVNDRRGLLE 240

Query: 241 ATGANIKADVVVAKDGSGKYTTITEALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTMTYV 300
           ATGANIKADVVVAKDGSGKY TITEALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTMTYV
Sbjct: 241 ATGANIKADVVVAKDGSGKYKTITEALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTMTYV 300

Query: 301 MMIGDGPTKTKITASANVIDGTPTFKSATFAAVGTNFIGKDLWFENSAGPEKHQAVALRV 360
           MMIGDGPTKTKITAS NV+DGTPTFKSATFAAVGTNFIGKDLWFENSAGPEKHQAVALRV
Sbjct: 301 MMIGDGPTKTKITASKNVVDGTPTFKSATFAAVGTNFIGKDLWFENSAGPEKHQAVALRV 360

Query: 361 QSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRKPMK 420
           QSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRKPMK
Sbjct: 361 QSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRKPMK 420

Query: 421 GQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDYYPVRHTIKSFLGRPWKLYSRTVVMQSQ 480
           GQTCLVTAQGRTQRKEPTGI+LQNCQISSDPDYYPVRHTIKSFLGRPWK YSRTVVMQ Q
Sbjct: 421 GQTCLVTAQGRTQRKEPTGIILQNCQISSDPDYYPVRHTIKSFLGRPWKQYSRTVVMQCQ 480

Query: 481 IDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRGIKQITADHATKFAPGL 540
           IDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRGIKQITADHA KFAP L
Sbjct: 481 IDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRGIKQITADHAVKFAPAL 540

Query: 541 FIGGNQWVKPTGIPYSGGLMTI 562
           FIGGN+WVK TGIPYSGGLMTI
Sbjct: 541 FIGGNRWVKQTGIPYSGGLMTI 557

BLAST of Csor.00g251940 vs. ExPASy TrEMBL
Match: A0A6J1IE74 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472353 PE=3 SV=1)

HSP 1 Score: 949 bits (2453), Expect = 0.0
Identity = 460/563 (81.71%), Postives = 505/563 (89.70%), Query Frame = 0

Query: 1   MASDGCGCKNNKVALVGLLSLFLVALVNGVSGGGEDPGSSNAAIKTLCEPTTYRETCEEA 60
           MA DG GCKNNKVALVGLLSLFLVALVNG SG GED GSSN AIKT+CEPTTYRETCEEA
Sbjct: 1   MAYDGGGCKNNKVALVGLLSLFLVALVNGASGAGEDSGSSNVAIKTICEPTTYRETCEEA 60

Query: 61  LVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPTLRAAAADPMAQKAVDACDELMDYAI 120
           LVKSNVDSKDPRELIKAGFHFAIEQLK A+ NSPTL+ AA DPMA++A+D CDEL+DYAI
Sbjct: 61  LVKSNVDSKDPRELIKAGFHFAIEQLKAAIANSPTLKQAATDPMAKEAIDTCDELIDYAI 120

Query: 121 DDLLITFDKMANSFSLHQINNYLGNIRIWLSGALTYQETCLDGFENVPGDTGEKMKNLLK 180
           DDLL TFD++ +SFSL++++NYL N+RIWLSGALTYQETCLD FENV GDTGEKM NLLK
Sbjct: 121 DDLLTTFDQITDSFSLNRVDNYLKNLRIWLSGALTYQETCLDAFENVTGDTGEKMTNLLK 180

Query: 181 TSKEMTANGLVMVGEITSFVSSLWQALGLSK-GRQLRIDESSEEFQDEEPSWVNDKRGLL 240
           TSKEMTANGLVMVGE+TS VSSLW+ALGL + GRQLR  E  EE ++EEPSWVND+RGLL
Sbjct: 181 TSKEMTANGLVMVGEVTSLVSSLWKALGLPETGRQLRAKELDEEDEEEEPSWVNDRRGLL 240

Query: 241 EATGANIKADVVVAKDGSGKYTTITEALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTMTY 300
           +ATGA I+A+VVVAKDGSGKY TIT+ALQ+VP+KSN TFVIYVKEGVY+EQVMVDKTMT+
Sbjct: 241 QATGAKIRANVVVAKDGSGKYKTITQALQDVPRKSNKTFVIYVKEGVYQEQVMVDKTMTW 300

Query: 301 VMMIGDGPTKTKITASANVIDGTPTFKSATFAAVGTNFIGKDLWFENSAGPEKHQAVALR 360
           VMMIGDGPTKTKITA  N  DGT TFK+ATFAAVGTNFIGKDLWFENSAGPEKHQAVALR
Sbjct: 301 VMMIGDGPTKTKITAGKNFKDGTSTFKTATFAAVGTNFIGKDLWFENSAGPEKHQAVALR 360

Query: 361 VQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRKPM 420
           VQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGT+DFIFGNAAV+LQNCKIFVRKPM
Sbjct: 361 VQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTIDFIFGNAAVILQNCKIFVRKPM 420

Query: 421 KGQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDYYPVRHTIKSFLGRPWKLYSRTVVMQS 480
           + Q C+VTAQGR +RKEPTGIVLQNC ISSDP ++PVRH  K++LGRPWK YSRT++MQ 
Sbjct: 421 ENQKCIVTAQGRLERKEPTGIVLQNCAISSDPGFFPVRHVFKTYLGRPWKQYSRTIIMQC 480

Query: 481 QIDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRGIKQITADHATKFAPG 540
           QIDDLIQPEGWL WEG FALKTLFY EFDNRGPGAA QNRV WRGIKQIT  HA  F PG
Sbjct: 481 QIDDLIQPEGWLPWEGDFALKTLFYTEFDNRGPGAAKQNRVKWRGIKQITPKHAIDFTPG 540

Query: 541 LFIGGNQWVKPTGIPYSGGLMTI 562
           LFI GN W++ TG+PY  G+ TI
Sbjct: 541 LFIRGNPWIRRTGVPYYSGMATI 563

BLAST of Csor.00g251940 vs. ExPASy TrEMBL
Match: A0A6J1FTV0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111446779 PE=3 SV=1)

HSP 1 Score: 940 bits (2430), Expect = 0.0
Identity = 457/563 (81.17%), Postives = 501/563 (88.99%), Query Frame = 0

Query: 1   MASDGCGCKNNKVALVGLLSLFLVALVNGVSGGGEDPGSSNAAIKTLCEPTTYRETCEEA 60
           MA DG GCKNNK ALVGLLSLFLVALVNGVSG GED GSSN AIKTLCEPT YRETCEEA
Sbjct: 1   MAYDGGGCKNNKAALVGLLSLFLVALVNGVSGAGEDSGSSNVAIKTLCEPTKYRETCEEA 60

Query: 61  LVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPTLRAAAADPMAQKAVDACDELMDYAI 120
           LVKSNVDSKDPRELIKAGFHFAIEQLK A+ NSPTL+ A  DPMA++A+D CDELMDYAI
Sbjct: 61  LVKSNVDSKDPRELIKAGFHFAIEQLKAAIANSPTLKQATTDPMAKEAIDTCDELMDYAI 120

Query: 121 DDLLITFDKMANSFSLHQINNYLGNIRIWLSGALTYQETCLDGFENVPGDTGEKMKNLLK 180
           DDLL TFD++ +SFSL++++NYL N+RIWLSGALTYQETCLD FENV GDTGEKM NLLK
Sbjct: 121 DDLLTTFDQITDSFSLNRVDNYLKNLRIWLSGALTYQETCLDAFENVTGDTGEKMANLLK 180

Query: 181 TSKEMTANGLVMVGEITSFVSSLWQALGLSK-GRQLRIDESSEEFQDEEPSWVNDKRGLL 240
           TSKEMTANGLVMVGE+TS VSSLW+ALGL + GRQLR +E  EE + EEPSWVND+RGLL
Sbjct: 181 TSKEMTANGLVMVGEVTSLVSSLWKALGLPETGRQLRGEEPDEEDEGEEPSWVNDRRGLL 240

Query: 241 EATGANIKADVVVAKDGSGKYTTITEALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTMTY 300
           + TGA I+A+VVVAKDGSGKY TIT+ALQ+VP+KSN TFVIYVKEGVY+EQVMVDKTMT+
Sbjct: 241 QHTGAKIRANVVVAKDGSGKYKTITQALQDVPRKSNKTFVIYVKEGVYQEQVMVDKTMTW 300

Query: 301 VMMIGDGPTKTKITASANVIDGTPTFKSATFAAVGTNFIGKDLWFENSAGPEKHQAVALR 360
           VMMIGDGPTKTKIT   N  DGT TF++ATFAAVGTNFIGKDLWFENSAGPEKHQAVALR
Sbjct: 301 VMMIGDGPTKTKITGRKNFKDGTNTFQTATFAAVGTNFIGKDLWFENSAGPEKHQAVALR 360

Query: 361 VQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRKPM 420
           VQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGT+DFIFGNAAVVLQNCKIFVRKPM
Sbjct: 361 VQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTIDFIFGNAAVVLQNCKIFVRKPM 420

Query: 421 KGQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDYYPVRHTIKSFLGRPWKLYSRTVVMQS 480
           + Q C+VTAQGRT RKEPTGIVLQNC ISSDP ++PVRH  K++LGRPWK YSRT++MQ 
Sbjct: 421 ENQKCIVTAQGRTDRKEPTGIVLQNCAISSDPGFFPVRHVFKTYLGRPWKQYSRTIIMQC 480

Query: 481 QIDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRGIKQITADHATKFAPG 540
           QIDDLIQPEGWL WEG FAL+TLFY E+DNRGPGAA QNRV WRGIKQIT  HA  F PG
Sbjct: 481 QIDDLIQPEGWLPWEGDFALRTLFYTEYDNRGPGAAKQNRVKWRGIKQITPKHAIDFTPG 540

Query: 541 LFIGGNQWVKPTGIPYSGGLMTI 562
           LFI GN W++ TG+PY  G+ TI
Sbjct: 541 LFIRGNPWIRRTGVPYYSGMATI 563

BLAST of Csor.00g251940 vs. ExPASy TrEMBL
Match: A0A6J1GBF1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111452639 PE=3 SV=1)

HSP 1 Score: 925 bits (2390), Expect = 0.0
Identity = 452/564 (80.14%), Postives = 496/564 (87.94%), Query Frame = 0

Query: 1   MASDGCGCKNNKVALVGLLSLFLVALVNGVSGGGEDPGSSNAAIKTLCEPTTYRETCEEA 60
           MA DG GCKNNK ALVGLLSLF VALVNGVSG GE+PGSSNAAIKTLCEPTTYRETCEE 
Sbjct: 1   MAYDGGGCKNNKAALVGLLSLFFVALVNGVSGAGEEPGSSNAAIKTLCEPTTYRETCEEV 60

Query: 61  LVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPTLRAAAADPMAQKAVDACDELMDYAI 120
           LVKSNVDSKDPREL+KAGFHFAIEQLK A+ NSPTL+ AA DPMAQKAVDACDELMDYAI
Sbjct: 61  LVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMDYAI 120

Query: 121 DDLLITFDKMANSFSLHQINNYLGNIRIWLSGALTYQETCLDGFENVPGDTGEKMKNLLK 180
           DDLL +FDK+ +SF +H++N YL N+RIWLS ALTYQETCLDGFENVPGDTGEKMKNLLK
Sbjct: 121 DDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLK 180

Query: 181 TSKEMTANGLVMVGEITSFVSSLWQALGL--SKGRQLRIDESSEEFQDEEPSWVNDKRGL 240
           TS+EMTANGLVMVGE+TS VS+LW+ LGL    GRQLR +ES+E +Q  EPSWV+D+RGL
Sbjct: 181 TSREMTANGLVMVGEVTSLVSTLWEKLGLLPQSGRQLRTEESNE-YQAGEPSWVSDRRGL 240

Query: 241 LEATGANIKADVVVAKDGSGKYTTITEALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTMT 300
           LEATGANIKA+VVVAKDGSGKY TITEAL+EVP KSNTTFVIYVKEGVYEEQV+VDK MT
Sbjct: 241 LEATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVLVDKKMT 300

Query: 301 YVMMIGDGPTKTKITASANVIDGTPTFKSATFAAVGTNFIGKDLWFENSAGPEKHQAVAL 360
           YVM+IGDGPTKTKITAS NVIDGTPTFKSATFAA+G+NFIGKDLWF+NSAGP+KHQAVAL
Sbjct: 301 YVMVIGDGPTKTKITASKNVIDGTPTFKSATFAAIGSNFIGKDLWFDNSAGPKKHQAVAL 360

Query: 361 RVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRKP 420
           RVQSDMSIFYNCRMD YQDTLY    RQF+RDCTI+GTVDFIFGNAAVVLQNCKI VRKP
Sbjct: 361 RVQSDMSIFYNCRMDAYQDTLYPQTKRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKP 420

Query: 421 MKGQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDYYPVRHTIKSFLGRPWKLYSRTVVMQ 480
           M  QTC VTAQGR +  EPTGIVLQNC IS+DP++YP+RHT K FLGRPWK YSRTV+MQ
Sbjct: 421 MPNQTCFVTAQGRVEIDEPTGIVLQNCIISADPEFYPIRHTSKIFLGRPWKKYSRTVIMQ 480

Query: 481 SQIDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRGIKQITADHATKFAP 540
            Q+DDLI PEGW+ W     LKTLFY EFDNRGPGAA +NRV W GIKQITA  A  F P
Sbjct: 481 CQLDDLIHPEGWMRWSDDSTLKTLFYTEFDNRGPGAAKENRVKWEGIKQITAKQAINFTP 540

Query: 541 GLFIGGNQWVKPTGIPYSGGLMTI 562
            LFI G+ W+K +GIPY+GG+M I
Sbjct: 541 SLFIQGDDWIKHSGIPYTGGMMKI 563

BLAST of Csor.00g251940 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 553.9 bits (1426), Expect = 1.5e-157
Identity = 269/536 (50.19%), Postives = 373/536 (69.59%), Query Frame = 0

Query: 28  NGVSGGGEDPGSSNAAIKTLCEPTTYRETCEEALVKSNVDSKDPRELIKAGFHFAIEQLK 87
           +G S G  +  +S  A+K +C PT YR+TCE+ L+K+  ++ DP EL+K  F+  ++Q+ 
Sbjct: 42  DGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQIT 101

Query: 88  VALQNSPTLRAAAADPMAQKAVDACDELMDYAIDDLLITFDKMANSFSLHQINNYLGNIR 147
            A + S T+     D   + A+D C ELMDYA+D+L  +F+++   F  H ++  L N+R
Sbjct: 102 DAAKKSQTIMELQKDSRTRMALDQCKELMDYALDELSNSFEEL-GKFEFHLLDEALINLR 161

Query: 148 IWLSGALTYQETCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEITSFVSSLWQAL 207
           IWLS A++++ETCL+GF+   G+ GE MK  LKT+ E+T NGL ++ E+++FV  + Q  
Sbjct: 162 IWLSAAISHEETCLEGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQM-QIP 221

Query: 208 GLSKGRQLRIDESSEEFQDEEPSWVNDK-RGLLEATGA--NIKADVVVAKDGSGKYTTIT 267
           GL+  R L     +E F    PSWV+ + R LL+A  A  ++K D+VVA+DGSG+Y TI 
Sbjct: 222 GLNSRRLL-----AEGF----PSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTIN 281

Query: 268 EALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTMTYVMMIGDGPTKTKITASANVIDGTPT 327
           EALQ VPKK NTTFV+++K G+Y+E V V+KTM++++ IGDGP KT I+ + N  DG  T
Sbjct: 282 EALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITT 341

Query: 328 FKSATFAAVGTNFIGKDLWFENSAGPEKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAH 387
           +++AT A VG  FI K++ FEN+AG  KHQAVA+RVQSD SIF+NCR DGYQDTLYTH+H
Sbjct: 342 YRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSH 401

Query: 388 RQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRKPMKGQTCLVTAQGRTQRKEPTGIVLQN 447
           RQFFRDCTI+GT+DF+FG+AA V QNC + VRKP+  Q C +TA GR   +E TG V Q 
Sbjct: 402 RQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQG 461

Query: 448 CQISSDPDYYPVRHTIKSFLGRPWKLYSRTVVMQSQIDDLIQPEGWLAWEGTFALKTLFY 507
           C I+ +PDY  V+ T K++LGRPWK YSRT++M + I D +QP+GW  W G F LKTLFY
Sbjct: 462 CTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFY 521

Query: 508 AEFDNRGPGAATQNRVTWRGIKQITADHATKFAPGLFIGGNQWVKPTGIPYSGGLM 561
           +E  N GPG+A  NRVTW GIK ++ +   KF P  +I G+ W+   G+PY+ GL+
Sbjct: 522 SEVQNTGPGSALANRVTWAGIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

BLAST of Csor.00g251940 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 536.2 bits (1380), Expect = 3.2e-152
Identity = 263/564 (46.63%), Postives = 375/564 (66.49%), Query Frame = 0

Query: 9   KNNKVALVGLLSLFLVALVNGV----------SGGGEDPGSSNAAIKTLCEPTTYRETCE 68
           +  +  ++ + S+ L+++V  V          + G E+  +S  AIK +C PT Y+ETCE
Sbjct: 12  RKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCE 71

Query: 69  EALVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPTLRAAAADPMAQKAVDACDELMDY 128
           + L K   D+ DP EL+K  F+  ++Q+    + S T+     DP A+ A+D C ELMDY
Sbjct: 72  DTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMALDQCKELMDY 131

Query: 129 AIDDLLITFDKMANSFSLHQINNYLGNIRIWLSGALTYQETCLDGFENVPGDTGEKMKNL 188
           AI +L  +F+++   F  H+++  L  +RIWLS  +++++TCLDGF+   G+ GE +K  
Sbjct: 132 AIGELSKSFEEL-GKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKA 191

Query: 189 LKTSKEMTANGLVMVGEITSFVSSLWQALGLSKGRQLRIDESSEEFQDEEPSWVNDK-RG 248
           LKT+ ++T NGL MV E+++++  + Q   ++  R L     S+EF    PSW++ + R 
Sbjct: 192 LKTAVQLTHNGLAMVTEMSNYLGQM-QIPEMNSRRLL-----SQEF----PSWMDARARR 251

Query: 249 LLEATGANIKADVVVAKDGSGKYTTITEALQEVPKKSNTTFVIYVKEGVYEEQVMVDKTM 308
           LL A  + +K D+VVA+DGSG+Y TI EAL  VPKK NTTFV+++KEG+Y+E V V+++M
Sbjct: 252 LLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSM 311

Query: 309 TYVMMIGDGPTKTKITASANVIDGTPTFKSATFAAVGTNFIGKDLWFENSAGPEKHQAVA 368
           T+++ IGDGP KT I+ S +  DG  T+K+AT A VG +FI K++ FEN+AG  KHQAVA
Sbjct: 312 THLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVA 371

Query: 369 LRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNAAVVLQNCKIFVRK 428
           +RV +D SIFYNC+ DGYQDTLY H+HRQF+RDCTI+GT+DF+FG+AA V QNC + VRK
Sbjct: 372 IRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRK 431

Query: 429 PMKGQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDYYPVRHTIKSFLGRPWKLYSRTVVM 488
           P+  Q C +TA GR   +E TG VLQ C I  +PDY  V+   K++LGRPWK YSRT++M
Sbjct: 432 PLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIM 491

Query: 489 QSQIDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRGIKQITADHATKFA 548
            + I D + PEGW  W G F L TLFY+E  N GPGAA   RVTW GIK+++ +   KF 
Sbjct: 492 NTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFT 551

Query: 549 PGLFIGGNQWVKPTGIPYSGGLMT 562
           P  +I G+ W+   G+PY  GL +
Sbjct: 552 PAQYIQGDAWIPGKGVPYILGLFS 564

BLAST of Csor.00g251940 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 525.8 bits (1353), Expect = 4.3e-149
Identity = 259/524 (49.43%), Postives = 360/524 (68.70%), Query Frame = 0

Query: 39  SSNAAIKTLCEPTTYRETCEEALVKSNVDSKDPRELIKAGFHFAIEQLKVALQN-SPTLR 98
           ++  A++ +C PT Y+ETC  +L+K++ DS  P +LIK GF+  I  ++ +++  S  L 
Sbjct: 50  TATTAVEAVCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELT 109

Query: 99  AAAA-DPMAQKAVDACDELMDYAIDDLLITFDKMANSFSLHQINNYLGNIRIWLSGALTY 158
           A AA D   + A++ C++LM+ A DDL    D   + FS+ QI +++ ++R+WLSG++ Y
Sbjct: 110 AKAANDKDTKGALELCEKLMNDATDDLKKCLDNF-DGFSIPQIEDFVEDLRVWLSGSIAY 169

Query: 159 QETCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEITSFVSSLWQALGLS--KGRQ 218
           Q+TC+D FE       + M+ + KTS+E+T+NGL M+  I++ +   +   G++   G+ 
Sbjct: 170 QQTCMDTFEETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGE-FNVTGVTGDLGKY 229

Query: 219 LRIDESSEEFQDEEPSWVNDKRGLLEATGANIKADVVVAKDGSGKYTTITEALQEVPKKS 278
            R   S+E   D  PSWV      L AT   +KA+VVVA DGSG+Y TI EAL  VPK +
Sbjct: 230 ARKLLSAE---DGIPSWVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPKAN 289

Query: 279 NTTFVIYVKEGVYEEQVMVDKTMTYVMMIGDGPTKTKITASANVIDG-TPTFKSATFAAV 338
              FVIY+K+GVY E+V V K MT+V  IGDGPTKTKIT S N   G   T+ +AT A  
Sbjct: 290 QKPFVIYIKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAIN 349

Query: 339 GTNFIGKDLWFENSAGPEKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTI 398
           G NF  K++ FEN+AGPE HQAVALRV +D+++FYNC++DGYQDTLY H+HRQFFRDCT+
Sbjct: 350 GDNFTAKNIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTV 409

Query: 399 TGTVDFIFGNAAVVLQNCKIFVRKPMKGQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDY 458
           +GTVDFIFG+  VVLQNC I VRKPMK Q+C++TAQGR+ ++E TG+VLQNC I+ +P Y
Sbjct: 410 SGTVDFIFGDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAY 469

Query: 459 YPVRHTIKSFLGRPWKLYSRTVVMQSQIDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPG 518
            PV+   K++LGRPWK +SRT++M + IDD+I P GWL W G FAL TL+YAE++N GPG
Sbjct: 470 IPVKSINKAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPG 529

Query: 519 AATQNRVTWRGIKQITADHATKFAPGLFIGGNQWVKPTGIPYSG 558
           +    RV W GIK+++   A +F P  F+ GN W+ P  +PY G
Sbjct: 530 SNQAQRVKWPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPYMG 568

BLAST of Csor.00g251940 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 477.2 bits (1227), Expect = 1.8e-134
Identity = 250/572 (43.71%), Postives = 365/572 (63.81%), Query Frame = 0

Query: 1   MASDGCGCKNNKVALVGLLSLFLVALVNGVS------GGGEDPG---SSNAAIKTLCEPT 60
           M SD  G K  K  + G +S FLV +V  V+         +D      +  A++ +C PT
Sbjct: 1   MGSD--GDKKKKFIVAGSVSGFLVIMVVSVAVVTSKHSPKDDENHIRKTTKAVQAVCAPT 60

Query: 61  TYRETCEEALVKSNVDSKDPRELIKAGFHFAIEQLKVALQNSPTLRAAAAD--PMAQKAV 120
            +++TC  +L+ ++ DS DP +LIK GF   I+ +  +L+ +     A AD  P A+ A 
Sbjct: 61  DFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKGAF 120

Query: 121 DACDELMDYAIDDLLITFDKMANSFSLHQINNYLGNIRIWLSGALTYQETCLDGFENVPG 180
           + C++LM  AIDDL    D   + FS+ QI  ++ ++R+WLSG++ +Q+TC+D F  +  
Sbjct: 121 ELCEKLMIDAIDDLKKCMD---HGFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIKS 180

Query: 181 DTGEKMKNLLKTSKEMTANGLVMVGEITSFVSSLWQALGLSKGRQLRIDESSEEFQDEEP 240
           +  + M  + KTS+E+++N L MV  I++ + +     GL+ G   +        +D  P
Sbjct: 181 NLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNS-NLTGLT-GALAKYARKLLSTEDSIP 240

Query: 241 SWVN-DKRGLLEATGAN---IKADVVVAKDGSGKYTTITEALQEVPKKSNTTFVIYVKEG 300
           +WV  + R L+ A G     +KA+ VVA+DG+G++ TIT+AL  VPK +   F+I++KEG
Sbjct: 241 TWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFIIHIKEG 300

Query: 301 VYEEQVMVDKTMTYVMMIGDGPTKTKITASANV-IDGTPTFKSATFAAVGTNFIGKDLWF 360
           +Y+E+V V K M +V  IGDGP KT IT S N  I    TF +AT    G +F  K++  
Sbjct: 301 IYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNIGI 360

Query: 361 ENSAGPEKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTVDFIFGNA 420
           EN+AGPE  QAVALRV +D ++F++C++DG+QDTLY H+HRQF+RDCT++GTVDFIFG+A
Sbjct: 361 ENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFGDA 420

Query: 421 AVVLQNCKIFVRKPMKGQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDYYPVRHTIKSFL 480
             +LQNCKI VRKP KGQTC+VTAQGR+  +E TG+VL  C I+ DP Y P++   K++L
Sbjct: 421 KCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKAYL 480

Query: 481 GRPWKLYSRTVVMQSQIDDLIQPEGWLAWEGTFALKTLFYAEFDNRGPGAATQNRVTWRG 540
           GRPWK +SRT++M++ IDD+I P GWL W G FALKTL+YAE  N GPG+    RV W G
Sbjct: 481 GRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKWPG 540

Query: 541 IKQITADHATKFAPGLFIGGNQWVKPTGIPYS 557
           IK++T   A  +    F+ G+ W+  T +PY+
Sbjct: 541 IKKLTPQDALLYTGDRFLRGDTWIPQTQVPYT 565

BLAST of Csor.00g251940 vs. TAIR 10
Match: AT4G15980.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 427.9 bits (1099), Expect = 1.2e-119
Identity = 249/701 (35.52%), Postives = 361/701 (51.50%), Query Frame = 0

Query: 11  NKVALVGLLSLFLVALVNGVSGGGEDPGS-------------SNAAIKTLCEPTTYRETC 70
           NK  L+G+ +L +  ++   +  G  P               +   +  +C  T Y++ C
Sbjct: 2   NKYVLLGVTALIMAMVICVEANDGNSPSRKMEEVHRESKLMITKTTVSIICASTDYKQDC 61

Query: 71  EEALVKSNVDSKDPRELIKAGFHFAIEQLKVALQNS--PTLRAAAADPMAQKAVDACDEL 130
             +L  + V S DPR LI++ F  AI  ++  +          A AD   ++A++ C EL
Sbjct: 62  TTSL--ATVRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTCREL 121

Query: 131 MDYAIDDLLITFDKMANSFSLHQINNYLGNIRIWLSGALTYQETCLDGFENVPGDTGEKM 190
           MD AIDDL  T DK    F   ++++++ ++ +WLSG++TYQ+TC+DGFE +  +    M
Sbjct: 122 MDDAIDDLRKTRDKF-RGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAVMM 181

Query: 191 KNLLKTSKEMTANGLVMVGEITSFV----------------------------------- 250
           + +++  + +T+NGL +   +   +                                   
Sbjct: 182 ERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSSQD 241

Query: 251 ---------------------------------SSLWQALGLSKGRQLR----------- 310
                                            SS  + L  SK +Q+            
Sbjct: 242 SPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKSAF 301

Query: 311 -----IDESSEEF----------------------------------------------- 370
                +D+SS++                                                
Sbjct: 302 SGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSLKD 361

Query: 371 -----QDEEPSWVN-DKRGLLEATGAN--IKADVVVAKDGSGKYTTITEALQEVPKKSNT 430
                + E P WV    R LL     N  IKA+VVVAKDGSGK  TI +AL  VP K+  
Sbjct: 362 RHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVPMKNTK 421

Query: 431 TFVIYVKEGVYEEQVMVDKTMTYVMMIGDGPTKTKITAS-ANVIDGTPTFKSATFAAVGT 490
            FVI++KEGVY+E+V V K M +VM +GDGPTKT IT   A + D   T+++A+ A  G 
Sbjct: 422 KFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAVNGD 481

Query: 491 NFIGKDLWFENSAGPEKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITG 550
            F+ KD+ FEN+AG  +HQAVALRV +D ++F+NC M+GYQDTLY H HRQF+R+C ++G
Sbjct: 482 YFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCRVSG 541

Query: 551 TVDFIFGNAAVVLQNCKIFVRKPMKGQTCLVTAQGRTQRKEPTGIVLQNCQISSDPDYYP 557
           T+DF+FG+A  V QNC+  +R+PM+ Q C+VTAQGR  R+E TGIV+ N +I+ D  Y P
Sbjct: 542 TIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDASYLP 601