Csor.00g248040 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g248040
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	bifunctional nuclease 2
Location	Csor_Chr06: 736406 .. 739240 (+)
RNA-Seq Expression	Csor.00g248040
Synteny	Csor.00g248040

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCACCCTTCTACCTCCTCTTCCCTCCACTTCTCTCTCCTTCGATCGCGATCCATTGCTTTTCGCTCTCGCCGCTCCAACTCGATACTCATTTCTTGCCACTCTTCTCCCGATAGATTCGCCCACACTGATGATGACTCCCGTCGGGATTATTTTGAAGCTTCGTTTCTCGTCACAGGTTCTTAATCTTTCACTTGGCTACTTTTTTGTTTTACTTTGATGTTTGTTGCAATTGCCGATCGAATCGATGGGGTGTTTTGTGATTTCTGTGGTTGTTTGTGTATTACTGACTGTTAGAGACTATCAATCACTATCGAATGTGGAAGAAGAGGTTTCAAGAGGATGCGAGTTTCAATCAGAGAGAATCGCGAGTCAATTCCCATTCACTTGGATTGGGGTTTTTTAGACGTTTTCGAAGCCCTACTGTGTTTCTCAAGATTTCTTGCAATGGTGACTTTCTACTGCCAATTGTCGTAGGTATGTTGGAGAAAAACTGCGACGTGATTCGGTTTTGTGTTTTCTTATGAATTTGTCAAAAAGAAGCTGCAAATTTTGGACTTGGTTTGCAGGGGAATATGCTATTGAGAAACTTATAGATTGTCAGCTTGGGATTGAAAATGGGGTGGGTTTTCTCTGGTTTTGTTCTTCATATATATATATTCATATATGTGTAGCTACCTTGTTGTTGGTATTAATTCTTAAATGGTCTCATATTACAGGATGCCCCTGATCTCTTCCAGTTTATACAAGACCTCATTGTGAAAGTTGGCTATCAAGTATGAACTGACTTCTAACATACTGAATAAATATATGCTTAGGATTTATTGTTTATCAGTCTTTGATGGGGAGGATTTATGAAACAAATGTTTTTTTCGAGAGTTCAATAGGGGTTTGAACTCTTGACCTCTTAGTCGAGGATATATGTCTTAACTAGTTGAGCGATTCTTGAACTGAGGTTTGTGACATTAATGTTAAGAACATTAGGATGGAATTTTTTTTTTTTTTTTCAGATTTTGATTCTTCAATAGCTTGAAAAGTGAATTGTTGCTTGCTACAATCATACATGGAATAATCAAGAAATATATACATGGCTTGTTAGTTTTTTCTATACAAGAACCAATGAGCTGCAAAAATCTGCTAGTTTTAATGTTGCTTTGCGATGTATGTTTACCTGATAGGACAAGCTTATGCTGGAGCAAGAATATTAGTGTCTTTGCCCAGTTGTGAGATCCCACATCCGTTGGAGAGGGGAACACAACATTCCTTATAAGGGTGTGGAAACCTCTCCCTAGCAGATGCGTTTTAGAATTGTGAGGCTGACAGCGATACGTAACGAGCCAAAACGGACATCGGGCAATGTGCCAGCGAGGACGTTGAGCCCCCAAGGGGGGTGGATTATGAGATTCCACATATGTTGGAGAGGGGAACGAAGCATTCCTTATAAGAGTGTGGAAACCTCTCCCTAGCAGATGCGTTTTAAAATTGTGAGGCTGACGGTGATACGCAACAGGCGAAAAGCGGACAATATCTGCTAGCGGTGGGCTTGGGCTGTTACACTTGCTTTCGAGGAATTATATAATTTGGGATTTAATTCTTCATCTAGTCATAGAATTATTTTCTCTAAGCTATATTATGGGTGGGCAGTGATTACTGATAGGAACCCTCCTTCCTTCACTTGTTTCTTTAATGTAATTGAGGATGAAAATCTAGAAGCACTGCACAGTTTCTGAATAAATCATTTGTACTTCTCATGTTCATTTCCTTTTTTGATAACCATGAAAAGGGGTTTAGAGCGATAATCTAATGCCATTTTATATTATCATGAGATAGGTCACCACAGCGAGAATTACTGAACGAGTGATGAACACTTACTTCGCCCGGCTCTTCTTGAAAAAGGTTAGCTCTTATAAACTCATTCACAATCAGCCAATACATTTTTAGAAGTTCTCAATACATGCCTTGTCTAACTATTATCATCTGCAGGATGGGGAAAATGAAACGTTAAGTGTGGATGCACGCCCCTCAGACGCCCTAAACGTTGCATACCGATGTAAGGTAGATATTCTACCATTAACTGCTGGGATCCTTGCAGAACTTCTACTAGTCGCGAGAATGACTTAGATTCTTTACTTTGCACCCTCTCCGCCCGATTTCATTCTCTTGACCGAAATAATTTCTTACTTCACTGAAAGTTTGCTGCTCACGGTGTTTTTCGGTATAACAAATGTGCAGATACCTGTACTTGTTAGTAAGCAGATTGTCTATGAGGATGCCATTAGAGTTAGTTATGGGTTTGGAAGAGTGCGTGAGAGGAAATCATGTTATGACGTTCTACTCGACAGGTAGAAGTGCATGTATTGTTTGCTATTTGATGAACATCTTTTCTTGATATCCAAAAACAATCATGTTCGTTTCGATCAGTGCTGCTGATGGTCCGGATTTCTTGTCCGAGGAACTCGATATGCTGAAGAATATGAAAATGGCTATTTACGAAGAACGGTACAAAGATGCAGGTATCTATCCAAACATTTTGTTCTTTATCATGTTTCTACAAATGAGTGTATTTATTTATAGCTCATGTTTGTTTCCTTCAGCCTTGTGGAGGGATAAGTTAACAAAGCTTCGCAAGTCCGAAGACGAGGAGTAACTCTGTGTAATCGACTGCTCTAAAAAGAGGAATCGTCGTGTACACGACGAAACATGTCTTAATTTTTCTGATACTGCACCCTAAACAGGGCGATGTTTCGGTCTCTTGGAACATACAATTCAGCATTGGATGAATTGGCAACGCTGAGCAAAGCGATGCGCATCCGTGCAGAAAAATTTCGATAG

mRNA sequence

ATGGATCACCCTTCTACCTCCTCTTCCCTCCACTTCTCTCTCCTTCGATCGCGATCCATTGCTTTTCGCTCTCGCCGCTCCAACTCGATACTCATTTCTTGCCACTCTTCTCCCGATAGATTCGCCCACACTGATGATGACTCCCGTCGGGATTATTTTGAAGCTTCGTTTCTCGTCACAGAGACTATCAATCACTATCGAATGTGGAAGAAGAGGTTTCAAGAGGATGCGAGTTTCAATCAGAGAGAATCGCGAGTCAATTCCCATTCACTTGGATTGGGGTTTTTTAGACGTTTTCGAAGCCCTACTGTGTTTCTCAAGATTTCTTGCAATGGTGACTTTCTACTGCCAATTGTCGTAGGGGAATATGCTATTGAGAAACTTATAGATTGTCAGCTTGGGATTGAAAATGGGGATGCCCCTGATCTCTTCCAGTTTATACAAGACCTCATTGTGAAAGTTGGCTATCAAGTCACCACAGCGAGAATTACTGAACGAGTGATGAACACTTACTTCGCCCGGCTCTTCTTGAAAAAGGATGGGGAAAATGAAACGTTAAGTGTGGATGCACGCCCCTCAGACGCCCTAAACGTTGCATACCGATGTAAGATACCTGTACTTGTTAGTAAGCAGATTGTCTATGAGGATGCCATTAGAGTTAGTTATGGGTTTGGAAGAGTGCGTGAGAGGAAATCATGTTATGACGTTCTACTCGACAGTGCTGCTGATGGTCCGGATTTCTTGTCCGAGGAACTCGATATGCTGAAGAATATGAAAATGGCTATTTACGAAGAACGGTACAAAGATGCAGCCTTGTGGAGGGATAAGTTAACAAAGCTTCGCAAGGCGATGTTTCGGTCTCTTGGAACATACAATTCAGCATTGGATGAATTGGCAACGCTGAGCAAAGCGATGCGCATCCGTGCAGAAAAATTTCGATAG

Coding sequence (CDS)

ATGGATCACCCTTCTACCTCCTCTTCCCTCCACTTCTCTCTCCTTCGATCGCGATCCATTGCTTTTCGCTCTCGCCGCTCCAACTCGATACTCATTTCTTGCCACTCTTCTCCCGATAGATTCGCCCACACTGATGATGACTCCCGTCGGGATTATTTTGAAGCTTCGTTTCTCGTCACAGAGACTATCAATCACTATCGAATGTGGAAGAAGAGGTTTCAAGAGGATGCGAGTTTCAATCAGAGAGAATCGCGAGTCAATTCCCATTCACTTGGATTGGGGTTTTTTAGACGTTTTCGAAGCCCTACTGTGTTTCTCAAGATTTCTTGCAATGGTGACTTTCTACTGCCAATTGTCGTAGGGGAATATGCTATTGAGAAACTTATAGATTGTCAGCTTGGGATTGAAAATGGGGATGCCCCTGATCTCTTCCAGTTTATACAAGACCTCATTGTGAAAGTTGGCTATCAAGTCACCACAGCGAGAATTACTGAACGAGTGATGAACACTTACTTCGCCCGGCTCTTCTTGAAAAAGGATGGGGAAAATGAAACGTTAAGTGTGGATGCACGCCCCTCAGACGCCCTAAACGTTGCATACCGATGTAAGATACCTGTACTTGTTAGTAAGCAGATTGTCTATGAGGATGCCATTAGAGTTAGTTATGGGTTTGGAAGAGTGCGTGAGAGGAAATCATGTTATGACGTTCTACTCGACAGTGCTGCTGATGGTCCGGATTTCTTGTCCGAGGAACTCGATATGCTGAAGAATATGAAAATGGCTATTTACGAAGAACGGTACAAAGATGCAGCCTTGTGGAGGGATAAGTTAACAAAGCTTCGCAAGGCGATGTTTCGGTCTCTTGGAACATACAATTCAGCATTGGATGAATTGGCAACGCTGAGCAAAGCGATGCGCATCCGTGCAGAAAAATTTCGATAG

Protein sequence

MDHPSTSSSLHFSLLRSRSIAFRSRRSNSILISCHSSPDRFAHTDDDSRRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLFLKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDVLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLRKAMFRSLGTYNSALDELATLSKAMRIRAEKFR

Homology

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BLAST of Csor.00g248040 vs. ExPASy Swiss-Prot
Match: Q93VH2 (Bifunctional nuclease 2 OS=Arabidopsis thaliana OX=3702 GN=BBD2 PE=2 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 5.4e-29
Identity = 70/183 (38.25%), Postives = 111/183 (60.66%), Query Frame = 0


Query: 104 VFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARI 163
           + LK+      LLPI+V E     L+     ++    P ++Q ++D++ K+GY+V   R+
Sbjct: 140 IVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIA-RPTMYQVVKDMVDKMGYEVRLVRV 199

Query: 164 TERVMNTYFARLFLKKDGE-NETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSY 223
           T RV   YFA L+L K G+ ++ +S D RPSDA+N+A RCK+P+ V+K + Y D +RV  
Sbjct: 200 TTRVHEAYFAELYLSKVGDKSDCVSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRV-I 259

Query: 224 GFGRVRERKSCYDVLLDSAADGPD----FLSEELDMLKNMKMAIYEERYKDAALWRDKLT 282
             G++ ++    D LL +  D P+    F ++E D+++NM  A+ EERY +AA WRDKL 
Sbjct: 260 DSGKLSKQTPASDGLLFTELDRPNGQPCFDTKEFDLVRNMMQAVDEERYDEAAEWRDKLG 319

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BLAST of Csor.00g248040 vs. ExPASy Swiss-Prot
Match: Q6YZM6 (Bifunctional nuclease 2 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD2 PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.6e-28
Identity = 75/201 (37.31%), Postives = 113/201 (56.22%), Query Frame = 0


Query: 86  VNSHSLGLGFFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQ 145
           V+++S G         P + L+I    + LLPI+V E     L+     +     P ++Q
Sbjct: 120 VHNNSQGRNIPESAPQPAIVLRIEDGSETLLPIIVLEMPSVLLMAAIRNVHIA-RPTIYQ 179

Query: 146 FIQDLIVKVGYQVTTARITERVMNTYFARLFLKKDGEN-ETLSVDARPSDALNVAYRCKI 205
            ++++I K+GY+V   RI +R+   Y A LFL K G++ E+++ D RPSDA+N+A RCK+
Sbjct: 180 VVKEMIDKMGYEVKLVRINKRIQEAYCAELFLTKVGDHTESITFDLRPSDAINIAVRCKV 239

Query: 206 PVLVSKQIVYEDAIRVSYGFGRVRERKSCYDVLLDSAADGPD----FLSEELDMLKNMKM 265
           P+ V + + Y D IR S    R+       D LL +  D PD      ++E  +++NM +
Sbjct: 240 PIQVHRSLAYSDGIR-SVEPARMAIAAGMSDGLLFTELDRPDGQPCVEAQEFGLIRNMLI 299

Query: 266 AIYEERYKDAALWRDKLTKLR 282
           A  EERYKDAA WRDKL  LR
Sbjct: 300 AAVEERYKDAATWRDKLMLLR 318

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BLAST of Csor.00g248040 vs. ExPASy Swiss-Prot
Match: Q9FWS6 (Bifunctional nuclease 1 OS=Arabidopsis thaliana OX=3702 GN=BBD1 PE=2 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 4.6e-28
Identity = 71/185 (38.38%), Postives = 108/185 (58.38%), Query Frame = 0


Query: 102 PTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTA 161
           P + LK+      LLPI+V E     L+     ++    P ++Q +++++ K+GY+V   
Sbjct: 134 PAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIA-RPTMYQVVKEMVDKMGYEVRLV 193

Query: 162 RITERVMNTYFARLFLKKDGE-NETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRV 221
           R+T+RV   YFA+LFL K G  +E +S D RPSDA+N+A RCKIP+ V+K + Y D +RV
Sbjct: 194 RVTKRVHEAYFAQLFLSKVGNASECVSFDLRPSDAINIAVRCKIPIQVNKYLAYSDGMRV 253

Query: 222 SYGFGRVRERKSCYDVLLDSAADGPD----FLSEELDMLKNMKMAIYEERYKDAALWRDK 281
               G++       D LL +  D P+      ++E ++L  M  A+ EERY +AA WRDK
Sbjct: 254 IES-GKISTPAPASDGLLFTEQDRPNGQACLDTKEFNILSKMMQAVDEERYDEAAEWRDK 313

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BLAST of Csor.00g248040 vs. ExPASy Swiss-Prot
Match: Q5N8J3 (Bifunctional nuclease 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD1 PE=2 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 6.6e-27
Identity = 69/182 (37.91%), Postives = 109/182 (59.89%), Query Frame = 0


Query: 104 VFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARI 163
           + LK+    D LLPI+V E     L+     I     P ++  ++++  ++GY V   RI
Sbjct: 145 IVLKMEDGSDLLLPIIVMETPSIMLLAALRNIRI-PRPTIYNVVKEMTERMGYAVRLVRI 204

Query: 164 TERVMNTYFARLFLKKDG-ENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRV-- 223
           TE V + Y++RL+L K G E ET+S+D +PSDA+N+A+RCK+P+ V+++I Y + ++V  
Sbjct: 205 TEMVHDAYYSRLYLAKIGNEEETISLDLKPSDAINIAFRCKVPIQVNRRIAYNNGLKVVQ 264

Query: 224 -SYGFGRVRERKSCYDVLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTK 282
            +     V   +  Y   LD   D P F ++E D+++NM +A  EERYKDAA +RD+L  
Sbjct: 265 PTPSESYVSSDQFQY-TRLDRPDDQPCFEAQEFDLVRNMLVAAVEERYKDAAQYRDQLFM 324

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BLAST of Csor.00g248040 vs. ExPASy Swiss-Prot
Match: Q09LL3 (Bifunctional nuclease OS=Oryza minuta OX=63629 GN=BBD PE=2 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 6.6e-27
Identity = 69/182 (37.91%), Postives = 109/182 (59.89%), Query Frame = 0


Query: 104 VFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARI 163
           + LK+    D LLPI+V E     L+     I     P ++  ++++  ++GY V   RI
Sbjct: 145 IVLKMEDGSDLLLPIIVMETPSIMLLAALRNIRI-PRPTIYNVVKEMTERMGYAVRLVRI 204

Query: 164 TERVMNTYFARLFLKKDG-ENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRV-- 223
           TE V + Y++RL+L K G E ET+S+D +PSDA+N+A+RCK+P+ V+++I Y + ++V  
Sbjct: 205 TEMVHDAYYSRLYLAKIGNEEETISLDLKPSDAINIAFRCKVPIQVNRRIAYNNGLKVVQ 264

Query: 224 -SYGFGRVRERKSCYDVLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTK 282
            +     V   +  Y   LD   D P F ++E D+++NM +A  EERYKDAA +RD+L  
Sbjct: 265 PTPSESYVSSDQFQY-TRLDRPDDQPCFEAQEFDLVRNMLVAAVEERYKDAAQYRDQLFM 324

[Close]

BLAST of Csor.00g248040 vs. NCBI nr
Match: KAG6596262.1 (Bifunctional nuclease 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 620 bits (1600), Expect = 4.95e-224
Identity = 313/313 (100.00%), Postives = 313/313 (100.00%), Query Frame = 0


Query: 1   MDHPSTSSSLHFSLLRSRSIAFRSRRSNSILISCHSSPDRFAHTDDDSRRDYFEASFLVT 60
           MDHPSTSSSLHFSLLRSRSIAFRSRRSNSILISCHSSPDRFAHTDDDSRRDYFEASFLVT
Sbjct: 1   MDHPSTSSSLHFSLLRSRSIAFRSRRSNSILISCHSSPDRFAHTDDDSRRDYFEASFLVT 60

Query: 61  ETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFRSPTVFLKISCNGDFLLPIVV 120
           ETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFRSPTVFLKISCNGDFLLPIVV
Sbjct: 61  ETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFRSPTVFLKISCNGDFLLPIVV 120

Query: 121 GEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLFLKKD 180
           GEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLFLKKD
Sbjct: 121 GEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLFLKKD 180

Query: 181 GENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDVLLDS 240
           GENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDVLLDS
Sbjct: 181 GENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDVLLDS 240

Query: 241 AADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLRKAMFRSLGTYNSALDELAT 300
           AADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLRKAMFRSLGTYNSALDELAT
Sbjct: 241 AADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLRKAMFRSLGTYNSALDELAT 300

Query: 301 LSKAMRIRAEKFR 313
           LSKAMRIRAEKFR
Sbjct: 301 LSKAMRIRAEKFR 313

[Close]

BLAST of Csor.00g248040 vs. NCBI nr
Match: XP_022951431.1 (bifunctional nuclease 2 [Cucurbita moschata] >KAG7027810.1 Bifunctional nuclease 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 564 bits (1454), Expect = 7.10e-202
Identity = 282/283 (99.65%), Postives = 283/283 (100.00%), Query Frame = 0


Query: 1   MDHPSTSSSLHFSLLRSRSIAFRSRRSNSILISCHSSPDRFAHTDDDSRRDYFEASFLVT 60
           MDHPSTSSSLHFSLLRSRSIAFRSRRSNSILISCHSSPDRFAHTDDDSRRDYFEASFLVT
Sbjct: 21  MDHPSTSSSLHFSLLRSRSIAFRSRRSNSILISCHSSPDRFAHTDDDSRRDYFEASFLVT 80

Query: 61  ETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFRSPTVFLKISCNGDFLLPIVV 120
           ETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFRSPTVFLKISCNGDFLLPIVV
Sbjct: 81  ETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFRSPTVFLKISCNGDFLLPIVV 140

Query: 121 GEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLFLKKD 180
           GEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLFLKKD
Sbjct: 141 GEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLFLKKD 200

Query: 181 GENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDVLLDS 240
           GENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDVLLDS
Sbjct: 201 GENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDVLLDS 260

Query: 241 AADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLRKA 283
           AADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLRK+
Sbjct: 261 AADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLRKS 303

[Close]

BLAST of Csor.00g248040 vs. NCBI nr
Match: XP_022971524.1 (bifunctional nuclease 2-like [Cucurbita maxima])

HSP 1 Score: 536 bits (1380), Expect = 1.55e-190
Identity = 271/287 (94.43%), Postives = 276/287 (96.17%), Query Frame = 0


Query: 1   MDHPSTS----SSLHFSLLRSRSIAFRSRRSNSILISCHSSPDRFAHTDDDSRRDYFEAS 60
           MDHPSTS    S LHFSL RS SIA RSRRSNSILISCHSSPD FAHTDDDS RDYFEAS
Sbjct: 21  MDHPSTSRPSSSFLHFSLPRSPSIALRSRRSNSILISCHSSPDGFAHTDDDSHRDYFEAS 80

Query: 61  FLVTETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFRSPTVFLKISCNGDFLL 120
           FLVTETINHYRMWKKRFQEDASFNQRES+VNSHSLGLGFFRRFRSPTVFLKISCNGDFLL
Sbjct: 81  FLVTETINHYRMWKKRFQEDASFNQRESQVNSHSLGLGFFRRFRSPTVFLKISCNGDFLL 140

Query: 121 PIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLF 180
           PIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLF
Sbjct: 141 PIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLF 200

Query: 181 LKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDV 240
           LKKDGE ETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYD+
Sbjct: 201 LKKDGEYETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDI 260

Query: 241 LLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLRKA 283
           LLDSAADGP+FLSEELDMLKNMKMAIYEERYKDAA+WRDKLTKLRK+
Sbjct: 261 LLDSAADGPNFLSEELDMLKNMKMAIYEERYKDAAVWRDKLTKLRKS 307

[Close]

BLAST of Csor.00g248040 vs. NCBI nr
Match: XP_038905904.1 (bifunctional nuclease 2 [Benincasa hispida] >XP_038905905.1 bifunctional nuclease 2 [Benincasa hispida] >XP_038905906.1 bifunctional nuclease 2 [Benincasa hispida])

HSP 1 Score: 496 bits (1277), Expect = 7.85e-175
Identity = 251/289 (86.85%), Postives = 268/289 (92.73%), Query Frame = 0


Query: 1   MDHPSTS---SSLHFSLLRSRSIAFRSRRSNSILISCHSSPDRFAHT-DDDSRRDYFEAS 60
           MDHPS S   SSLHFS+LRSR I  RSRR  SILISCHSS DR  H+ DDDS +DYFEAS
Sbjct: 21  MDHPSASRPSSSLHFSVLRSRPIPVRSRRPKSILISCHSSRDRSTHSHDDDSHQDYFEAS 80

Query: 61  FLVTETINHYRMWKKRFQEDASFNQRES-RVNSHSLGLGFFRRFRSPTVFLKISCNGDFL 120
           FLV+ETI HYRMWKKRFQEDA+FNQRES RVNSHSLGLGFFRRFRSPTVFLKISCNGDFL
Sbjct: 81  FLVSETIYHYRMWKKRFQEDATFNQRESSRVNSHSLGLGFFRRFRSPTVFLKISCNGDFL 140

Query: 121 LPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARL 180
           LPIVVGEYAIEKLIDCQLGIENG+APD+FQFIQDLIVKVGY+ TTARITERV+NTYFARL
Sbjct: 141 LPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVGYEATTARITERVVNTYFARL 200

Query: 181 FLKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYD 240
           FL+K+GENE LSVDARPSDALN+AYRCKIPVLVSKQIV+ED IRVSYGFGRV ERKSC+D
Sbjct: 201 FLRKEGENEMLSVDARPSDALNIAYRCKIPVLVSKQIVFEDGIRVSYGFGRVHERKSCFD 260

Query: 241 VLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLRKAM 284
           VLLD AADGPDFLSEELDMLKNMKMAIYEERYKDAA+WRDKLTKLRK++
Sbjct: 261 VLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKLTKLRKSV 309

[Close]

BLAST of Csor.00g248040 vs. NCBI nr
Match: XP_022965292.1 (bifunctional nuclease 2-like [Cucurbita maxima] >XP_022965293.1 bifunctional nuclease 2-like [Cucurbita maxima] >XP_022965294.1 bifunctional nuclease 2-like [Cucurbita maxima])

HSP 1 Score: 495 bits (1275), Expect = 1.47e-174
Identity = 248/287 (86.41%), Postives = 267/287 (93.03%), Query Frame = 0


Query: 1   MDHPSTS---SSLHFSLLRSRSIAFRSRRSNSILISCHSSPDRFAHTDDDSRRDYFEASF 60
           MDHPS S   SSLHFS+LRSR  A RSRR  SIL+SCHSS DR  H+DDDS +DYFEASF
Sbjct: 21  MDHPSASRPSSSLHFSVLRSRPNAVRSRRPKSILVSCHSSRDRSTHSDDDSHQDYFEASF 80

Query: 61  LVTETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFRSPTVFLKISCNGDFLLP 120
           LV+ETI HYRMWKKRFQED +FNQ ESRVNS SLGLGFFRRF+SPTVFLKISCNGDFLLP
Sbjct: 81  LVSETIYHYRMWKKRFQEDTNFNQIESRVNSPSLGLGFFRRFQSPTVFLKISCNGDFLLP 140

Query: 121 IVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLFL 180
           IVVGEYAIEKLID  LGIENG+APDLFQFIQDL+VKVGY+VTTARITERV+NTYFARLFL
Sbjct: 141 IVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYEVTTARITERVVNTYFARLFL 200

Query: 181 KKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDVL 240
           +KDGENETLSVDARPSDALN+AYRCKIPVLVSKQIVYEDAIRVS+GFGRVR+RKSC+DVL
Sbjct: 201 RKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDAIRVSFGFGRVRKRKSCFDVL 260

Query: 241 LDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLRKAM 284
           LD AADGPDFLSEELDMLKNMKMAIYEERYKDAA+WRDKLTKLRK++
Sbjct: 261 LDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKLTKLRKSV 307

[Close]

BLAST of Csor.00g248040 vs. ExPASy TrEMBL
Match: A0A6J1GHK4 (bifunctional nuclease 2 OS=Cucurbita moschata OX=3662 GN=LOC111454246 PE=3 SV=1)

HSP 1 Score: 564 bits (1454), Expect = 3.44e-202
Identity = 282/283 (99.65%), Postives = 283/283 (100.00%), Query Frame = 0


Query: 1   MDHPSTSSSLHFSLLRSRSIAFRSRRSNSILISCHSSPDRFAHTDDDSRRDYFEASFLVT 60
           MDHPSTSSSLHFSLLRSRSIAFRSRRSNSILISCHSSPDRFAHTDDDSRRDYFEASFLVT
Sbjct: 21  MDHPSTSSSLHFSLLRSRSIAFRSRRSNSILISCHSSPDRFAHTDDDSRRDYFEASFLVT 80

Query: 61  ETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFRSPTVFLKISCNGDFLLPIVV 120
           ETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFRSPTVFLKISCNGDFLLPIVV
Sbjct: 81  ETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFRSPTVFLKISCNGDFLLPIVV 140

Query: 121 GEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLFLKKD 180
           GEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLFLKKD
Sbjct: 141 GEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLFLKKD 200

Query: 181 GENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDVLLDS 240
           GENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDVLLDS
Sbjct: 201 GENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDVLLDS 260

Query: 241 AADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLRKA 283
           AADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLRK+
Sbjct: 261 AADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLRKS 303

[Close]

BLAST of Csor.00g248040 vs. ExPASy TrEMBL
Match: A0A6J1I8T8 (bifunctional nuclease 2-like OS=Cucurbita maxima OX=3661 GN=LOC111470219 PE=3 SV=1)

HSP 1 Score: 536 bits (1380), Expect = 7.51e-191
Identity = 271/287 (94.43%), Postives = 276/287 (96.17%), Query Frame = 0


Query: 1   MDHPSTS----SSLHFSLLRSRSIAFRSRRSNSILISCHSSPDRFAHTDDDSRRDYFEAS 60
           MDHPSTS    S LHFSL RS SIA RSRRSNSILISCHSSPD FAHTDDDS RDYFEAS
Sbjct: 21  MDHPSTSRPSSSFLHFSLPRSPSIALRSRRSNSILISCHSSPDGFAHTDDDSHRDYFEAS 80

Query: 61  FLVTETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFRSPTVFLKISCNGDFLL 120
           FLVTETINHYRMWKKRFQEDASFNQRES+VNSHSLGLGFFRRFRSPTVFLKISCNGDFLL
Sbjct: 81  FLVTETINHYRMWKKRFQEDASFNQRESQVNSHSLGLGFFRRFRSPTVFLKISCNGDFLL 140

Query: 121 PIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLF 180
           PIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLF
Sbjct: 141 PIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLF 200

Query: 181 LKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDV 240
           LKKDGE ETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYD+
Sbjct: 201 LKKDGEYETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDI 260

Query: 241 LLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLRKA 283
           LLDSAADGP+FLSEELDMLKNMKMAIYEERYKDAA+WRDKLTKLRK+
Sbjct: 261 LLDSAADGPNFLSEELDMLKNMKMAIYEERYKDAAVWRDKLTKLRKS 307

[Close]

BLAST of Csor.00g248040 vs. ExPASy TrEMBL
Match: A0A6J1HJX9 (bifunctional nuclease 2-like OS=Cucurbita maxima OX=3661 GN=LOC111465202 PE=3 SV=1)

HSP 1 Score: 495 bits (1275), Expect = 7.12e-175
Identity = 248/287 (86.41%), Postives = 267/287 (93.03%), Query Frame = 0


Query: 1   MDHPSTS---SSLHFSLLRSRSIAFRSRRSNSILISCHSSPDRFAHTDDDSRRDYFEASF 60
           MDHPS S   SSLHFS+LRSR  A RSRR  SIL+SCHSS DR  H+DDDS +DYFEASF
Sbjct: 21  MDHPSASRPSSSLHFSVLRSRPNAVRSRRPKSILVSCHSSRDRSTHSDDDSHQDYFEASF 80

Query: 61  LVTETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFRSPTVFLKISCNGDFLLP 120
           LV+ETI HYRMWKKRFQED +FNQ ESRVNS SLGLGFFRRF+SPTVFLKISCNGDFLLP
Sbjct: 81  LVSETIYHYRMWKKRFQEDTNFNQIESRVNSPSLGLGFFRRFQSPTVFLKISCNGDFLLP 140

Query: 121 IVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLFL 180
           IVVGEYAIEKLID  LGIENG+APDLFQFIQDL+VKVGY+VTTARITERV+NTYFARLFL
Sbjct: 141 IVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYEVTTARITERVVNTYFARLFL 200

Query: 181 KKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDVL 240
           +KDGENETLSVDARPSDALN+AYRCKIPVLVSKQIVYEDAIRVS+GFGRVR+RKSC+DVL
Sbjct: 201 RKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDAIRVSFGFGRVRKRKSCFDVL 260

Query: 241 LDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLRKAM 284
           LD AADGPDFLSEELDMLKNMKMAIYEERYKDAA+WRDKLTKLRK++
Sbjct: 261 LDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKLTKLRKSV 307

[Close]

BLAST of Csor.00g248040 vs. ExPASy TrEMBL
Match: A0A6J1E735 (bifunctional nuclease 2-like OS=Cucurbita moschata OX=3662 GN=LOC111431208 PE=3 SV=1)

HSP 1 Score: 493 bits (1268), Expect = 8.29e-174
Identity = 247/287 (86.06%), Postives = 266/287 (92.68%), Query Frame = 0


Query: 1   MDHPSTS---SSLHFSLLRSRSIAFRSRRSNSILISCHSSPDRFAHTDDDSRRDYFEASF 60
           MDHPS S   SSLHFS+LRSR  A  SRR  SIL+SCHSS DR  H+DDDS +DYFEASF
Sbjct: 21  MDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSSRDRSTHSDDDSHQDYFEASF 80

Query: 61  LVTETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFRSPTVFLKISCNGDFLLP 120
           LV+ETI HYRMWKKRFQEDA+FNQ ESRVNS  LGLGFFRRFRSPTVFLKISCNGDFLLP
Sbjct: 81  LVSETIYHYRMWKKRFQEDANFNQIESRVNSPLLGLGFFRRFRSPTVFLKISCNGDFLLP 140

Query: 121 IVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLFL 180
           IVVGEYAIEKLID  LGIENG+APDLFQFIQDL+VKVGY+VTTARITERV+NTYFARLFL
Sbjct: 141 IVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYEVTTARITERVVNTYFARLFL 200

Query: 181 KKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDVL 240
           +KDGENETLSVDARPSDALN+AYRCKIPVLVSKQIVYEDAIRVS+GFGRVR+RKSC+DVL
Sbjct: 201 RKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDAIRVSFGFGRVRKRKSCFDVL 260

Query: 241 LDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLRKAM 284
           LD AADGPDFLSEELDMLKNMKMAIYEERYKDAA+WRDKLT+LRK++
Sbjct: 261 LDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKLTRLRKSV 307

[Close]

BLAST of Csor.00g248040 vs. ExPASy TrEMBL
Match: A0A0A0L3L8 (BFN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002490 PE=3 SV=1)

HSP 1 Score: 488 bits (1255), Expect = 8.50e-172
Identity = 246/289 (85.12%), Postives = 265/289 (91.70%), Query Frame = 0


Query: 1   MDHPSTSS---SLHFSLLRSRSIAFRSRRSNSILISCHSSPDRFAHTDDD--SRRDYFEA 60
           MDHPS S    SLHFSL RSR  A RSRR  SILISCHSS DR  H+DDD  S +DYFEA
Sbjct: 21  MDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSSRDRSTHSDDDDNSHQDYFEA 80

Query: 61  SFLVTETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFRSPTVFLKISCNGDFL 120
           SFLV+ETI HYRMWKKRFQEDA+FNQRESR NS+SLGLGFFRRFRSPTVFLKISCNGDFL
Sbjct: 81  SFLVSETIYHYRMWKKRFQEDANFNQRESRANSNSLGLGFFRRFRSPTVFLKISCNGDFL 140

Query: 121 LPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARL 180
           LPIVVGEYAIEKLIDCQLGIENG+APD+FQFIQDLIVKVGY+  TARITERV+NTYFARL
Sbjct: 141 LPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVGYEAITARITERVVNTYFARL 200

Query: 181 FLKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYD 240
           FL+K+GE+E LSVDARPSDALN+AYRCKIPVLVSKQIV+EDAIRVSYGFGRV ERKSC+D
Sbjct: 201 FLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFEDAIRVSYGFGRVHERKSCFD 260

Query: 241 VLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLRKAM 284
           VLLD AADGPDFLSEELDMLKNMK+AIYEERYKDAA+WRDKLTKLRK++
Sbjct: 261 VLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRDKLTKLRKSV 309

[Close]

BLAST of Csor.00g248040 vs. TAIR 10
Match: AT5G66050.1 (Wound-responsive family protein )

HSP 1 Score: 235.0 bits (598), Expect = 8.5e-62
Identity = 124/287 (43.21%), Postives = 180/287 (62.72%), Query Frame = 0


Query: 21  AFRSRRSNSILISCHSSPDRFAHTDDDSRRDYFEASFLVTETINHYRMWKKRFQEDA--- 80
           + RS R   I   C            D   ++ +AS LV ET  HY+M +  F++D+   
Sbjct: 52  SLRSHRRPKISHICCDGSSISGSGLGDPDLEFLQASVLVAETSMHYKMRRHGFRQDSMWQ 111

Query: 81  --------SFNQRESRVNSHSLGLGFFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLI 140
                   S    ESRV    +GLGF R+F+ PT+FLKISC+GD+LLP++VG+ A+EKL+
Sbjct: 112 TSRPLPPFSIRASESRVGVLPIGLGFLRQFKQPTIFLKISCDGDYLLPVIVGDAAVEKLL 171

Query: 141 DCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLFLKKDGENETLSVD 200
           D  L     + PD FQF+  ++ K+GY+V   ++T R++NTY+A L L K G+ E + +D
Sbjct: 172 DVPLQGHTEECPDQFQFVSAVVDKLGYEVKMVKLTGRIVNTYYASLCLGKPGDIEAICID 231

Query: 201 ARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDVLLDSAADGPDFLS 260
           +RPSDA+NVA  C+ P+ V+K IV E+AI++ YG GR +  K  ++V+LDSA DGPD LS
Sbjct: 232 SRPSDAINVARACQAPIYVNKAIVLEEAIKIGYG-GRPQSAKPVFNVILDSAPDGPDPLS 291

Query: 261 EELDMLKNMKMAIYEERYKDAALWRDKLTKLRKAMFRSLGTYNSALD 297
           EEL +++NM +A  EERY DAA+WRD+L  L+ +   S   YN  ++
Sbjct: 292 EELKLVRNMDLASKEERYIDAAMWRDRLKNLQNS---SSVVYNKGVE 334

[Close]

BLAST of Csor.00g248040 vs. TAIR 10
Match: AT5G66050.2 (Wound-responsive family protein )

HSP 1 Score: 225.3 bits (573), Expect = 6.7e-59
Identity = 112/243 (46.09%), Postives = 164/243 (67.49%), Query Frame = 0


Query: 65  HYRMWKKRFQEDA-----------SFNQRESRVNSHSLGLGFFRRFRSPTVFLKISCNGD 124
           HY+M +  F++D+           S    ESRV    +GLGF R+F+ PT+FLKISC+GD
Sbjct: 2   HYKMRRHGFRQDSMWQTSRPLPPFSIRASESRVGVLPIGLGFLRQFKQPTIFLKISCDGD 61

Query: 125 FLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFA 184
           +LLP++VG+ A+EKL+D  L     + PD FQF+  ++ K+GY+V   ++T R++NTY+A
Sbjct: 62  YLLPVIVGDAAVEKLLDVPLQGHTEECPDQFQFVSAVVDKLGYEVKMVKLTGRIVNTYYA 121

Query: 185 RLFLKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSC 244
            L L K G+ E + +D+RPSDA+NVA  C+ P+ V+K IV E+AI++ YG GR +  K  
Sbjct: 122 SLCLGKPGDIEAICIDSRPSDAINVARACQAPIYVNKAIVLEEAIKIGYG-GRPQSAKPV 181

Query: 245 YDVLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLRKAMFRSLGTYNS 297
           ++V+LDSA DGPD LSEEL +++NM +A  EERY DAA+WRD+L  L+ +   S   YN 
Sbjct: 182 FNVILDSAPDGPDPLSEELKLVRNMDLASKEERYIDAAMWRDRLKNLQNS---SSVVYNK 240

[Close]

BLAST of Csor.00g248040 vs. TAIR 10
Match: AT1G19660.1 (Wound-responsive family protein )

HSP 1 Score: 129.8 bits (325), Expect = 3.8e-30
Identity = 70/183 (38.25%), Postives = 111/183 (60.66%), Query Frame = 0


Query: 104 VFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARI 163
           + LK+      LLPI+V E     L+     ++    P ++Q ++D++ K+GY+V   R+
Sbjct: 140 IVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIA-RPTMYQVVKDMVDKMGYEVRLVRV 199

Query: 164 TERVMNTYFARLFLKKDGE-NETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSY 223
           T RV   YFA L+L K G+ ++ +S D RPSDA+N+A RCK+P+ V+K + Y D +RV  
Sbjct: 200 TTRVHEAYFAELYLSKVGDKSDCVSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRV-I 259

Query: 224 GFGRVRERKSCYDVLLDSAADGPD----FLSEELDMLKNMKMAIYEERYKDAALWRDKLT 282
             G++ ++    D LL +  D P+    F ++E D+++NM  A+ EERY +AA WRDKL 
Sbjct: 260 DSGKLSKQTPASDGLLFTELDRPNGQPCFDTKEFDLVRNMMQAVDEERYDEAAEWRDKLG 319

[Close]

BLAST of Csor.00g248040 vs. TAIR 10
Match: AT1G19660.2 (Wound-responsive family protein )

HSP 1 Score: 129.8 bits (325), Expect = 3.8e-30
Identity = 70/183 (38.25%), Postives = 111/183 (60.66%), Query Frame = 0


Query: 104 VFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARI 163
           + LK+      LLPI+V E     L+     ++    P ++Q ++D++ K+GY+V   R+
Sbjct: 140 IVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIA-RPTMYQVVKDMVDKMGYEVRLVRV 199

Query: 164 TERVMNTYFARLFLKKDGE-NETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSY 223
           T RV   YFA L+L K G+ ++ +S D RPSDA+N+A RCK+P+ V+K + Y D +RV  
Sbjct: 200 TTRVHEAYFAELYLSKVGDKSDCVSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRV-I 259

Query: 224 GFGRVRERKSCYDVLLDSAADGPD----FLSEELDMLKNMKMAIYEERYKDAALWRDKLT 282
             G++ ++    D LL +  D P+    F ++E D+++NM  A+ EERY +AA WRDKL 
Sbjct: 260 DSGKLSKQTPASDGLLFTELDRPNGQPCFDTKEFDLVRNMMQAVDEERYDEAAEWRDKLG 319

[Close]

BLAST of Csor.00g248040 vs. TAIR 10
Match: AT1G75380.1 (bifunctional nuclease in basal defense response 1 )

HSP 1 Score: 126.7 bits (317), Expect = 3.2e-29
Identity = 71/185 (38.38%), Postives = 108/185 (58.38%), Query Frame = 0


Query: 102 PTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTA 161
           P + LK+      LLPI+V E     L+     ++    P ++Q +++++ K+GY+V   
Sbjct: 134 PAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIA-RPTMYQVVKEMVDKMGYEVRLV 193

Query: 162 RITERVMNTYFARLFLKKDGE-NETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRV 221
           R+T+RV   YFA+LFL K G  +E +S D RPSDA+N+A RCKIP+ V+K + Y D +RV
Sbjct: 194 RVTKRVHEAYFAQLFLSKVGNASECVSFDLRPSDAINIAVRCKIPIQVNKYLAYSDGMRV 253

Query: 222 SYGFGRVRERKSCYDVLLDSAADGPD----FLSEELDMLKNMKMAIYEERYKDAALWRDK 281
               G++       D LL +  D P+      ++E ++L  M  A+ EERY +AA WRDK
Sbjct: 254 IES-GKISTPAPASDGLLFTEQDRPNGQACLDTKEFNILSKMMQAVDEERYDEAAEWRDK 313

The following BLAST results are available for this feature:

BLAST of Csor.00g248040 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93VH2	5.4e-29	38.25	Bifunctional nuclease 2 OS=Arabidopsis thaliana OX=3702 GN=BBD2 PE=2 SV=1	[more]
Q6YZM6	1.6e-28	37.31	Bifunctional nuclease 2 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD2 PE=2 SV...	[more]
Q9FWS6	4.6e-28	38.38	Bifunctional nuclease 1 OS=Arabidopsis thaliana OX=3702 GN=BBD1 PE=2 SV=1	[more]
Q5N8J3	6.6e-27	37.91	Bifunctional nuclease 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD1 PE=2 SV...	[more]
Q09LL3	6.6e-27	37.91	Bifunctional nuclease OS=Oryza minuta OX=63629 GN=BBD PE=2 SV=1	[more]

BLAST of Csor.00g248040 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6596262.1	4.95e-224	100.00	Bifunctional nuclease 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022951431.1	7.10e-202	99.65	bifunctional nuclease 2 [Cucurbita moschata] >KAG7027810.1 Bifunctional nuclease...	[more]
XP_022971524.1	1.55e-190	94.43	bifunctional nuclease 2-like [Cucurbita maxima]	[more]
XP_038905904.1	7.85e-175	86.85	bifunctional nuclease 2 [Benincasa hispida] >XP_038905905.1 bifunctional nucleas...	[more]
XP_022965292.1	1.47e-174	86.41	bifunctional nuclease 2-like [Cucurbita maxima] >XP_022965293.1 bifunctional nuc...	[more]

BLAST of Csor.00g248040 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GHK4	3.44e-202	99.65	bifunctional nuclease 2 OS=Cucurbita moschata OX=3662 GN=LOC111454246 PE=3 SV=1	[more]
A0A6J1I8T8	7.51e-191	94.43	bifunctional nuclease 2-like OS=Cucurbita maxima OX=3661 GN=LOC111470219 PE=3 SV...	[more]
A0A6J1HJX9	7.12e-175	86.41	bifunctional nuclease 2-like OS=Cucurbita maxima OX=3661 GN=LOC111465202 PE=3 SV...	[more]
A0A6J1E735	8.29e-174	86.06	bifunctional nuclease 2-like OS=Cucurbita moschata OX=3662 GN=LOC111431208 PE=3 ...	[more]
A0A0A0L3L8	8.50e-172	85.12	BFN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002490 PE=3 SV...	[more]

BLAST of Csor.00g248040 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G66050.1	8.5e-62	43.21	Wound-responsive family protein	[more]
AT5G66050.2	6.7e-59	46.09	Wound-responsive family protein	[more]
AT1G19660.1	3.8e-30	38.25	Wound-responsive family protein	[more]
AT1G19660.2	3.8e-30	38.25	Wound-responsive family protein	[more]
AT1G75380.1	3.2e-29	38.38	bifunctional nuclease in basal defense response 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003729	Bifunctional nuclease domain	PFAM	PF02577	DUF151	coord: 113..220 e-value: 7.2E-17 score: 61.2
IPR003729	Bifunctional nuclease domain	PROSITE	PS51658	BFN	coord: 83..220 score: 27.090006
IPR036104	Bifunctional nuclease superfamily	GENE3D	3.10.690.10	Bifunctional nuclease domain	coord: 76..223 e-value: 3.7E-28 score: 99.9
IPR036104	Bifunctional nuclease superfamily	SUPERFAMILY	103256	Hypothetical protein TM0160	coord: 91..221
None	No IPR available	PANTHER	PTHR15160:SF1	VON HIPPEL-LINDAU DISEASE TUMOR SUPPRESSOR-RELATED	coord: 9..280
None	No IPR available	PANTHER	PTHR15160	VON HIPPEL-LINDAU PROTEIN	coord: 9..280

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g248040.m01	Csor.00g248040.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005634	nucleus
cellular_component	GO:0030891	VCB complex
molecular_function	GO:0004518	nuclease activity