Csor.00g245280 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g245280
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionRibonuclease T(2)
LocationCsor_Chr16: 1187568 .. 1190240 (+)
RNA-Seq ExpressionCsor.00g245280
SyntenyCsor.00g245280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGGTGGCGCCGGCGGTGTTTCTTCTTGTTCTTCAACTGGTGGCGGTGGTAATCGCGGCGGAGACTCCAAGCGGGGGCGAGCAGAGGGAATTTGACTACTTTGTGTTGGCTCTTCAATGGCCAGCTACTTCTTGCAAGAACACTGGAAAGTGTTGCCCGGCCAATGGTTGCTGCCGTGGGTGAGCTTTTGTCTCCGACATTAAGCTTGATCCAGACGTTGACTTTTGAAAGCTGACTTTTTTTGTGTTTTCTCTTGTGTTTGAATATTTTGTTTGTCAACTGTCTAGAGCTGACTCTCCCACTGAATTTACAATCCGTAAGTGGAAAACTAAGCCCTCTAAGGTTTGGAATTTGGATGTTTTGTGTATTAAGATCTGAAAAGTTTGTGTTTTGAATGTGACAGATGGGTTATGGCCTCAGTATAATGAGAAAGGATGGCCATCTTGTTGCACTGATGCAGTCTTCAATCAAAATGAGGTCTCTCTCTCGAACTTGAACTCGAATGAGTATAACAAATGATTCTACGATTGTTTGCTATGTTAAACGGCCTAAGTCCACTGCTAGTAGATATTGTCCATTTTGACTCATTACGTATCACTGTTACCTCACGGTTTTGAAACGTGTCTACTAGGGAGAGGTTTCCACACCCTTATAAATAATAATTCATTCTCCTCTCCAACCGATATGGGATCTCACAATCCATCTCTTTTGAGGGCCCAGGGTCCTCGCTGGCACACTACTCAGTGTCTAGCTTTGATTCCATTTGTAACCGTTCAAACTCACCGCTAGCAGATATTGTCTTCTTTAAACTTTCCTTTCTAGGCTTCTCCTCAAGATTTTAAAACGCATCTGTTAGGGGGAGGTTTCCACACCCTTATAAGAAATGCTTTATTCTCCTCTCCAACCAATGTGGAATCTCACAATCCACCCCCTTGAAGACCCAACGTCTTCACTGGCACACCGCTCGATGTCTAACTTTGATACCATTTGTAACAACTCAAGCCCACCATCGGAAGATATTGTCCTTTTTGGACTTTCCCTTATGGACTTCCTCTTAAAATTTTAAAACACGTAGGTTAGGAAAAGGTTTTCATACCCTTATAAGGAATGCAATCTCGAAACCAACACAATTTTCTCTTACCCGTTACTAAACACAATTACTTGAGTTCAACAATTATGTACAACAAATCAAACTCTTAATATTTAGAATGATAATTAGTGTCTTACCCATTTGCAGATTAGCTTGTTGAGGGAGGATATTGAAAAGTATTGGCCAACCTATAGATGTGGTACTACATCCAACTGCCATGGTACCAAAGGATCATTTTGGGCTCATGAGGTTCATATCACTCTCTCTTTGTTCTTCCTTAGCTCATCTGTCAAACTTTGAGACTAATCCCAAATTCCTTTCCTTTTTTTTCTTCCTCTTATATGATCGCCATATCAAATGCTACCATTGCTGCAGTATGGTCAGTTTCTTCTTCACTTCCAATCTTCTTGGAACTTGATATCTTGTTCTACTCAAATTCTAACCCAAACCCTCCATTTTCAGAGAAGCATGGGACCTGCGCAGCTCCTGTGATTGTCGGCGAATACGATTACTTCTTGACAACGCTCACCATCTTCTCCAAGTACAATGTCACGGTAAGTAATACATCTCTATTGGTACGAAGCCTTTTGGGGAAGCTCAAAGCAAAGCCACGAAAGCTTATACTGAAAATGAACAATATCATATCATTGTGGAGAGTCGTGATTACTAACTAGTATCAGAGTCATGTCTTAACTTAACCATGTCAATAGAATACTCAAATGTCGAACAAAGAAGTTGTAAGCCTCGAATGTGTAGTCAAAAGTGACTCAAGTGTCGAACAAAGGGTGTAGTTTGTTCGATGACTCCAGGGAAGGAGTCGAACTTCGATTAAGGGAAGGTTGTTTAAGGGCTCCATAGGCTTCAAGGGAGGCTCTATAGTGTACTTTGGTCGAAATACATCTTTGCTGGTATAAGGCTTTTTGGAAAGCCAGCCATAAAACTTAGGCTCAAAGTGAACAATATCATATACTATTGTGAAGAGTCGTAGTTTCTACGGGGTGAATATTAAATTTGGTGTCTTTTTCATTGTGGGTATTAAATTTGGTGTCTTTTTCTTTGTGGGTATCAAATTTGGTGTCTTTTTCGTCGTGGGTATCAAATTTGGTGTCATTTTCGTTGTGGGTATTCAACTTGGTGTCTTTGTGGGTATTAAATTTGGTGTCTTTTTCGTTATGGGTATTAAATTTGGTGTCTTTTTCAATTTGTTTATACCAGAAAGCGCTGAGCGATGCTGGGTTCGTAGCTTCCAATACTGAGAAGTATCCACTAGAAGATGTGGTGGATGCAGTCAAGAATGCTACCAATTCAACTCCAAAGATTAGCTGTGCTAAAAAGGGGGCTGTCAAGGAGCTTTGGATATGCTTTGACAAGAAATTTGAGGTGTTCTTTGTGTTCATAACAATCATATCTAATAAGCTTTTGGGTTGGATCAAATTGTTCATAGTTTTTTGTTGTTGCAGCCACGTGATTGTGATCTTGCCAATTCATGCCCCGAGTTTATTAAGCTCCCAACGTATGAGCCTCAGGATGTGGTGGAGGGAGCTATTCCATGGATTGCTGATGCTGAAGAACTTATTTAA

mRNA sequence

ATGGTGGTGGCGCCGGCGGTGTTTCTTCTTGTTCTTCAACTGGTGGCGGTGGTAATCGCGGCGGAGACTCCAAGCGGGGGCGAGCAGAGGGAATTTGACTACTTTGTGTTGGCTCTTCAATGGCCAGCTACTTCTTGCAAGAACACTGGAAAGTGTTGCCCGGCCAATGGTTGCTGCCGTGGAGCTGACTCTCCCACTGAATTTACAATCCATGGGTTATGGCCTCAGTATAATGAGAAAGGATGGCCATCTTGTTGCACTGATGCAGTCTTCAATCAAAATGAGATTAGCTTGTTGAGGGAGGATATTGAAAAGTATTGGCCAACCTATAGATGTGGTACTACATCCAACTGCCATGTATGGTCAGTTTCTTCTTCACTTCCAATCTTCTTGGAACTTGATATCTTGTTCTACTCAAATTCTAACCCAAACCCTCCATTTTCAGAGAAGCATGGGACCTGCGCAGCTCCTGTGATTGTCGGCGAATACGATTACTTCTTGACAACGCTCACCATCTTCTCCAAGTACAATAAAGCGCTGAGCGATGCTGGGTTCGTAGCTTCCAATACTGAGAAGTATCCACTAGAAGATGTGGTGGATGCAGTCAAGAATGCTACCAATTCAACTCCAAAGATTAGCTGTGCTAAAAAGGGGGCTGTCAAGGAGCTTTGGATATGCTTTGACAAGAAATTTGAGCCACGTGATTGTGATCTTGCCAATTCATGCCCCGAGTTTATTAAGCTCCCAACGTATGAGCCTCAGGATGTGGTGGAGGGAGCTATTCCATGGATTGCTGATGCTGAAGAACTTATTTAA

Coding sequence (CDS)

ATGGTGGTGGCGCCGGCGGTGTTTCTTCTTGTTCTTCAACTGGTGGCGGTGGTAATCGCGGCGGAGACTCCAAGCGGGGGCGAGCAGAGGGAATTTGACTACTTTGTGTTGGCTCTTCAATGGCCAGCTACTTCTTGCAAGAACACTGGAAAGTGTTGCCCGGCCAATGGTTGCTGCCGTGGAGCTGACTCTCCCACTGAATTTACAATCCATGGGTTATGGCCTCAGTATAATGAGAAAGGATGGCCATCTTGTTGCACTGATGCAGTCTTCAATCAAAATGAGATTAGCTTGTTGAGGGAGGATATTGAAAAGTATTGGCCAACCTATAGATGTGGTACTACATCCAACTGCCATGTATGGTCAGTTTCTTCTTCACTTCCAATCTTCTTGGAACTTGATATCTTGTTCTACTCAAATTCTAACCCAAACCCTCCATTTTCAGAGAAGCATGGGACCTGCGCAGCTCCTGTGATTGTCGGCGAATACGATTACTTCTTGACAACGCTCACCATCTTCTCCAAGTACAATAAAGCGCTGAGCGATGCTGGGTTCGTAGCTTCCAATACTGAGAAGTATCCACTAGAAGATGTGGTGGATGCAGTCAAGAATGCTACCAATTCAACTCCAAAGATTAGCTGTGCTAAAAAGGGGGCTGTCAAGGAGCTTTGGATATGCTTTGACAAGAAATTTGAGCCACGTGATTGTGATCTTGCCAATTCATGCCCCGAGTTTATTAAGCTCCCAACGTATGAGCCTCAGGATGTGGTGGAGGGAGCTATTCCATGGATTGCTGATGCTGAAGAACTTATTTAA

Protein sequence

MVVAPAVFLLVLQLVAVVIAAETPSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLREDIEKYWPTYRCGTTSNCHVWSVSSSLPIFLELDILFYSNSNPNPPFSEKHGTCAAPVIVGEYDYFLTTLTIFSKYNKALSDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFEPRDCDLANSCPEFIKLPTYEPQDVVEGAIPWIADAEELI
Homology
BLAST of Csor.00g245280 vs. ExPASy Swiss-Prot
Match: P42814 (Ribonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=RNS2 PE=2 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 8.4e-63
Identity = 119/256 (46.48%), Postives = 161/256 (62.89%), Query Frame = 0

Query: 9   LLVLQLVAVVIAAET-PSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCRGADSPTE 68
           LL++  +A   A +       QREFDYF L+LQWP T C+ T  CC  N CCRG+D+PT+
Sbjct: 8   LLLVACIAGAFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQ 67

Query: 69  FTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLREDIEKYWPTYRCGTTSNCHVWSVSSSL 128
           FTIHGLWP YN+  WPSCC  + F + EIS L + +EKYWP+  CG+ S+C+    S   
Sbjct: 68  FTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGS--- 127

Query: 129 PIFLELDILFYSNSNPNPPFSEKHGTCAAPVIVGEYDYFLTTLTIFSKYN--KALSDAGF 188
                    F+ +        EKHGTC++PV   EY+YFLTTL ++ K+N    L  AG+
Sbjct: 128 ---------FWGHE------WEKHGTCSSPVFHDEYNYFLTTLNLYLKHNVTDVLYQAGY 187

Query: 189 VASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFEPRDC----DLAN- 248
           VASN+EKYPL  +V A++NA + TP++ C K+ A+ E+ ICF K F+PRDC    DL + 
Sbjct: 188 VASNSEKYPLGGIVTAIQNAFHITPEVVC-KRDAIDEIRICFYKDFKPRDCVGSQDLTSR 244

Query: 249 -SCPEFIKLPTYEPQD 256
            SCP+++ LP Y P D
Sbjct: 248 KSCPKYVSLPEYTPLD 244

BLAST of Csor.00g245280 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.2e-21
Identity = 67/227 (29.52%), Postives = 104/227 (45.81%), Query Frame = 0

Query: 10  LVLQLVAVVIAAETPSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCRGADSPTEFT 69
           L L  + V++ +   +     +FD+F    QWP + C    KCC  N     AD    F 
Sbjct: 8   LCLISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAAD----FG 67

Query: 70  IHGLWPQYNEKGWPS-CCTDAVFNQNEISLLREDIEKYWPTYRCGTTSNCHVWSVSSSLP 129
           IHGLWP Y +  +PS C     F+ + IS L   ++K WPT  C + S    W       
Sbjct: 68  IHGLWPNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHE---- 127

Query: 130 IFLELDILFYSNSNPNPPFSEKHGTCAAPVIVGEYDYFLTTLTIFSKYN--KALSDAGFV 189
                               EKHGTC+  VI  +++YF T L +  K N   AL+ AG +
Sbjct: 128 -------------------WEKHGTCSESVI-DQHEYFQTALNLKQKTNLLGALTKAG-I 187

Query: 190 ASNTEKYPLEDVVDAVKNATNSTPKISCAKKGA----VKELWICFDK 230
             + + Y LE + D++K +   TP + C + G+    + ++++C D+
Sbjct: 188 NPDGKSYSLESIRDSIKESIGFTPWVECNRDGSGNSQLYQVYLCVDR 205

BLAST of Csor.00g245280 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 6.1e-21
Identity = 66/230 (28.70%), Postives = 103/230 (44.78%), Query Frame = 0

Query: 7   VFLLVLQLVAVVIAAETPSGGEQREFDYFVLALQWPATSCKNTGKCC-PANGCCRGADSP 66
           +F+L LQ + V   A+        +FD+F   LQWP   C +   CC P  G        
Sbjct: 5   IFILALQQLYVQSFAQ--------DFDFFYFVLQWPGAYCDSRHSCCYPQTG-----KPA 64

Query: 67  TEFTIHGLWPQYNEKGWP-SCCTDAVFNQNEISLLREDIEKYWPTYRCGTTSNCHVWSVS 126
            +F IHGLWP Y   GWP +C  D+ F+   +S L  D+++ WPT  C +      W+  
Sbjct: 65  ADFGIHGLWPNYKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHE 124

Query: 127 SSLPIFLELDILFYSNSNPNPPFSEKHGTCAAPVIVGEYDYFLTTLTIFSKYN--KALSD 186
                                   EKHGTCA   +  ++DYF   L +  K N   AL++
Sbjct: 125 -----------------------WEKHGTCAESEL-DQHDYFEAGLKLKQKANLLHALTN 184

Query: 187 AGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGA----VKELWICFD 229
           AG +  + + Y ++D+ + +K      P I C K  +    + ++++C D
Sbjct: 185 AG-IKPDDKFYEMKDIENTIKQVVGFAPGIECNKDSSHNSQLYQIYLCVD 196

BLAST of Csor.00g245280 vs. ExPASy Swiss-Prot
Match: P80196 (Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2)

HSP 1 Score: 102.1 bits (253), Expect = 1.0e-20
Identity = 64/238 (26.89%), Postives = 103/238 (43.28%), Query Frame = 0

Query: 30  REFDYFVLALQWPATSCKNTGKCC-PANGCCRGADSPTEFTIHGLWPQYNEKGWP-SCCT 89
           ++FD+F    QWPA+ C     CC P  G         +F+IHGLWP Y +  WP +C  
Sbjct: 26  QDFDFFYFVQQWPASYCDTRRSCCYPTTG-----KPDEDFSIHGLWPNYKDGKWPQNCDR 85

Query: 90  DAVFNQNEISLLREDIEKYWPTYRCGTTSNCHVWSVSSSLPIFLELDILFYSNSNPNPPF 149
           ++  +++E S L   +EK WP+  C ++     WS                         
Sbjct: 86  ESSLDESEFSDLISTMEKNWPSLACPSSDGLKFWSHEWL--------------------- 145

Query: 150 SEKHGTCAAPVIVGEYDYFLTTLTIFSKYN--KALSDAGFVASNTEKYPLEDVVDAVKNA 209
             KHGTC+A   + ++ YF T L   +K N  + L++AG    N + Y +E +  A++  
Sbjct: 146 --KHGTCSA---LNQHAYFQTALDFKTKSNLLQNLNNAGIKPRNGDYYGVESIKKAIEKG 205

Query: 210 TNSTPKISC----AKKGAVKELWICFDKKFEP-RDCDL---ANSCPEFIKLPTYEPQD 256
              TP I C         + ++++C D       DC +      C   I+ P++   D
Sbjct: 206 VGHTPFIECNVDSQGNHQLYQVYLCVDSSASKFIDCPIFPHGGKCGSKIEFPSFSTND 232

BLAST of Csor.00g245280 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 99.8 bits (247), Expect = 5.2e-20
Identity = 67/255 (26.27%), Postives = 115/255 (45.10%), Query Frame = 0

Query: 7   VFLLVLQLVAVVIAAETPSGGEQREFDYFVLALQWPATSCKNTGKCC-PANGCCRGADSP 66
           + L++ Q ++V+ AA        ++FD+F    QWP + C     CC P  G        
Sbjct: 12  ILLIITQCLSVLNAA--------KDFDFFYFVQQWPGSYCDTKQSCCYPTTG-----KPA 71

Query: 67  TEFTIHGLWPQYNEKGWPS-CCTDAVFNQNEISLLREDIEKYWPTYRCGTTSNCHVWSVS 126
            +F IHGLWP  N+  +PS C  ++ ++Q++IS L   +++ WPT  C + S    WS  
Sbjct: 72  ADFGIHGLWPNNNDGTYPSNCDPNSPYDQSQISDLISSMQQNWPTLACPSGSGSTFWSHE 131

Query: 127 SSLPIFLELDILFYSNSNPNPPFSEKHGTCAAPVIVGEYDYFLTTLTIFSKYN-KALSDA 186
                                   EKHGTCA  V+  ++ YF   L + ++ +  ++   
Sbjct: 132 -----------------------WEKHGTCAESVLTNQHAYFKKALDLKNQIDLLSILQG 191

Query: 187 GFVASNTEKYPLEDVVDAVKNATNSTPKISC----AKKGAVKELWICFDKKFEPR-DCDL 246
             +  + E Y L ++ +A+K+A   TP I C    +    + +++IC D       +C +
Sbjct: 192 ADIHPDGESYDLVNIRNAIKSAIGYTPWIQCNVDQSGNSQLYQVYICVDGSGSSLIECPI 230

Query: 247 --ANSCPEFIKLPTY 252
                C   I+ PT+
Sbjct: 252 FPGGKCGTSIEFPTF 230

BLAST of Csor.00g245280 vs. NCBI nr
Match: KAG6576854.1 (Ribonuclease 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 573 bits (1478), Expect = 7.50e-207
Identity = 271/271 (100.00%), Postives = 271/271 (100.00%), Query Frame = 0

Query: 1   MVVAPAVFLLVLQLVAVVIAAETPSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCR 60
           MVVAPAVFLLVLQLVAVVIAAETPSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCR
Sbjct: 1   MVVAPAVFLLVLQLVAVVIAAETPSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCR 60

Query: 61  GADSPTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLREDIEKYWPTYRCGTTSNCHV 120
           GADSPTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLREDIEKYWPTYRCGTTSNCHV
Sbjct: 61  GADSPTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLREDIEKYWPTYRCGTTSNCHV 120

Query: 121 WSVSSSLPIFLELDILFYSNSNPNPPFSEKHGTCAAPVIVGEYDYFLTTLTIFSKYNKAL 180
           WSVSSSLPIFLELDILFYSNSNPNPPFSEKHGTCAAPVIVGEYDYFLTTLTIFSKYNKAL
Sbjct: 121 WSVSSSLPIFLELDILFYSNSNPNPPFSEKHGTCAAPVIVGEYDYFLTTLTIFSKYNKAL 180

Query: 181 SDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFEPRDCDLAN 240
           SDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFEPRDCDLAN
Sbjct: 181 SDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFEPRDCDLAN 240

Query: 241 SCPEFIKLPTYEPQDVVEGAIPWIADAEELI 271
           SCPEFIKLPTYEPQDVVEGAIPWIADAEELI
Sbjct: 241 SCPEFIKLPTYEPQDVVEGAIPWIADAEELI 271

BLAST of Csor.00g245280 vs. NCBI nr
Match: XP_022922895.1 (ribonuclease 2-like [Cucurbita moschata])

HSP 1 Score: 489 bits (1259), Expect = 1.47e-173
Identity = 239/273 (87.55%), Postives = 247/273 (90.48%), Query Frame = 0

Query: 1   MVVAPAVFLLVLQLVAVVIAAETPSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCR 60
           MVVAPAVFLLVLQLVAVV+AAETPSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCR
Sbjct: 11  MVVAPAVFLLVLQLVAVVLAAETPSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCR 70

Query: 61  GADSPTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLREDIEKYWPTYRCGTTSNCHV 120
           GADSPTEFTIHGLWPQYNEKGWPSCCTDAVF+QNEI+LL+EDIEKYWPTYRCGTTSNCH 
Sbjct: 71  GADSPTEFTIHGLWPQYNEKGWPSCCTDAVFSQNEITLLKEDIEKYWPTYRCGTTSNCHG 130

Query: 121 WSVSSSLPIFLELDILFYSNSNPNPPFSEKHGTCAAPVIVGEYDYFLTTLTIFSKYN--K 180
              S            F+++        EKHGTCAAPVIVGE+DYFLTTLTIFSKYN  K
Sbjct: 131 TKGS------------FWAHEY------EKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTK 190

Query: 181 ALSDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFEPRDCDL 240
           ALSDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFEPRDCDL
Sbjct: 191 ALSDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFEPRDCDL 250

Query: 241 ANSCPEFIKLPTYEPQDVVEGAIPWIADAEELI 271
           ANSCPEFIKLPTYEPQDVVEGAIPWIADAEELI
Sbjct: 251 ANSCPEFIKLPTYEPQDVVEGAIPWIADAEELI 265

BLAST of Csor.00g245280 vs. NCBI nr
Match: XP_022984766.1 (ribonuclease 2-like [Cucurbita maxima])

HSP 1 Score: 471 bits (1212), Expect = 2.30e-166
Identity = 236/275 (85.82%), Postives = 242/275 (88.00%), Query Frame = 0

Query: 1   MVVAPAVFLLV-LQLVAVVIAAETPSGGE-QREFDYFVLALQWPATSCKNTGKCCPANGC 60
           MVVAPAVFLL+ LQLVAVV+AAETPSGG  Q+EFDYFVLALQWPATSCKNTGKCCPANGC
Sbjct: 11  MVVAPAVFLLLALQLVAVVLAAETPSGGGGQKEFDYFVLALQWPATSCKNTGKCCPANGC 70

Query: 61  CRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLREDIEKYWPTYRCGTTSNC 120
           CRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLL EDIEKYWPTYRCGTTSNC
Sbjct: 71  CRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLSEDIEKYWPTYRCGTTSNC 130

Query: 121 HVWSVSSSLPIFLELDILFYSNSNPNPPFSEKHGTCAAPVIVGEYDYFLTTLTIFSKYN- 180
           H    S            F+++        EKHGTCAAPVIVGEYDYFLTTLTIFSKYN 
Sbjct: 131 HGTKGS------------FWAHEY------EKHGTCAAPVIVGEYDYFLTTLTIFSKYNV 190

Query: 181 -KALSDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFEPRDC 240
            KALSDAGFVASNTEKYPL DVVDAVKNATNSTPKISCAKKGAVKELWICFDK FEPRDC
Sbjct: 191 TKALSDAGFVASNTEKYPLGDVVDAVKNATNSTPKISCAKKGAVKELWICFDKNFEPRDC 250

Query: 241 DLANSCPEFIKLPTYEPQDVVEGAIPWIADAEELI 271
           DLANSCPEFIKLPTYEPQ VVEGAIPWIADAEELI
Sbjct: 251 DLANSCPEFIKLPTYEPQGVVEGAIPWIADAEELI 267

BLAST of Csor.00g245280 vs. NCBI nr
Match: XP_023553229.1 (ribonuclease 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 466 bits (1199), Expect = 2.04e-164
Identity = 228/273 (83.52%), Postives = 238/273 (87.18%), Query Frame = 0

Query: 1   MVVAPAVFLLVLQLVAVVIAAETPSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCR 60
           MVVAPAVFLLVLQLVAVV+AAETPSGG QREFDYFVLALQWPATSCKNTGKCCPANGCCR
Sbjct: 11  MVVAPAVFLLVLQLVAVVLAAETPSGGGQREFDYFVLALQWPATSCKNTGKCCPANGCCR 70

Query: 61  GADSPTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLREDIEKYWPTYRCGTTSNCHV 120
           GADSPTEFTIHGLWPQYNE+GWPSCCTDAVFNQNEISLL EDI+KYWPTYRCGTT NCH 
Sbjct: 71  GADSPTEFTIHGLWPQYNERGWPSCCTDAVFNQNEISLLEEDIQKYWPTYRCGTTGNCHG 130

Query: 121 WSVSSSLPIFLELDILFYSNSNPNPPFSEKHGTCAAPVIVGEYDYFLTTLTIFSKYN--K 180
              S            F+++        EKHGTCAAPVIVGEYDYFLTTLTIFSKYN  K
Sbjct: 131 TKGS------------FWAHEY------EKHGTCAAPVIVGEYDYFLTTLTIFSKYNVTK 190

Query: 181 ALSDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFEPRDCDL 240
           ALSDAGFVASNTEKY L DVVDAVKNAT+STPKISCAKKGAVKELWICFDK FEPRDCDL
Sbjct: 191 ALSDAGFVASNTEKYALGDVVDAVKNATDSTPKISCAKKGAVKELWICFDKNFEPRDCDL 250

Query: 241 ANSCPEFIKLPTYEPQDVVEGAIPWIADAEELI 271
           ANSCP+FIKLPTYE Q V+EGAIPWIADAE L+
Sbjct: 251 ANSCPDFIKLPTYESQGVMEGAIPWIADAEALV 265

BLAST of Csor.00g245280 vs. NCBI nr
Match: XP_038876700.1 (ribonuclease 2-like [Benincasa hispida])

HSP 1 Score: 420 bits (1079), Expect = 4.17e-146
Identity = 205/274 (74.82%), Postives = 223/274 (81.39%), Query Frame = 0

Query: 4   APAVFLLVLQLVAVVIA--AETPSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCRG 63
           AP VFLL LQL+AV     A  PSG EQREFDYFVLALQWPATSCKN GKCCPANGCCRG
Sbjct: 13  APVVFLL-LQLLAVAFGFTAVAPSGSEQREFDYFVLALQWPATSCKNPGKCCPANGCCRG 72

Query: 64  ADSPTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLREDIEKYWPTYRCGTTSNCHVW 123
            DSPTEFTIHGLWPQYN++GWPSCCTDA FN+NEI++L EDI+KYWPTYRCGTTS CH  
Sbjct: 73  VDSPTEFTIHGLWPQYNDRGWPSCCTDANFNENEINILAEDIQKYWPTYRCGTTSTCHRT 132

Query: 124 SVSSSLPIFLELDILFYSNSNPNPPFSEKHGTCAAPVIVGEYDYFLTTLTIFSKYN--KA 183
             S            F+++        EKHGTCAAPVIVGEYDYFLTTLTIFSKYN  K 
Sbjct: 133 KGS------------FWAHEY------EKHGTCAAPVIVGEYDYFLTTLTIFSKYNVTKV 192

Query: 184 LSDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFEPRDCDLA 243
           L+DAGFVASNTEKYP+EDVV A+KN  NSTPKI+CAKKG VKELWICFDK FEPRDCDL 
Sbjct: 193 LNDAGFVASNTEKYPIEDVVAAIKNEFNSTPKITCAKKGVVKELWICFDKSFEPRDCDLQ 252

Query: 244 NSCPEFIKLPTYEPQDV--VEGAIPWIADAEELI 271
           NSCP+FIKLPTY+PQ+   VEGAIPWIADAEE+I
Sbjct: 253 NSCPDFIKLPTYDPQEPNKVEGAIPWIADAEEII 267

BLAST of Csor.00g245280 vs. ExPASy TrEMBL
Match: A0A6J1EA39 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111430737 PE=3 SV=1)

HSP 1 Score: 489 bits (1259), Expect = 7.11e-174
Identity = 239/273 (87.55%), Postives = 247/273 (90.48%), Query Frame = 0

Query: 1   MVVAPAVFLLVLQLVAVVIAAETPSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCR 60
           MVVAPAVFLLVLQLVAVV+AAETPSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCR
Sbjct: 11  MVVAPAVFLLVLQLVAVVLAAETPSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCR 70

Query: 61  GADSPTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLREDIEKYWPTYRCGTTSNCHV 120
           GADSPTEFTIHGLWPQYNEKGWPSCCTDAVF+QNEI+LL+EDIEKYWPTYRCGTTSNCH 
Sbjct: 71  GADSPTEFTIHGLWPQYNEKGWPSCCTDAVFSQNEITLLKEDIEKYWPTYRCGTTSNCHG 130

Query: 121 WSVSSSLPIFLELDILFYSNSNPNPPFSEKHGTCAAPVIVGEYDYFLTTLTIFSKYN--K 180
              S            F+++        EKHGTCAAPVIVGE+DYFLTTLTIFSKYN  K
Sbjct: 131 TKGS------------FWAHEY------EKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTK 190

Query: 181 ALSDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFEPRDCDL 240
           ALSDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFEPRDCDL
Sbjct: 191 ALSDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFEPRDCDL 250

Query: 241 ANSCPEFIKLPTYEPQDVVEGAIPWIADAEELI 271
           ANSCPEFIKLPTYEPQDVVEGAIPWIADAEELI
Sbjct: 251 ANSCPEFIKLPTYEPQDVVEGAIPWIADAEELI 265

BLAST of Csor.00g245280 vs. ExPASy TrEMBL
Match: A0A6J1J670 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111482949 PE=3 SV=1)

HSP 1 Score: 471 bits (1212), Expect = 1.11e-166
Identity = 236/275 (85.82%), Postives = 242/275 (88.00%), Query Frame = 0

Query: 1   MVVAPAVFLLV-LQLVAVVIAAETPSGGE-QREFDYFVLALQWPATSCKNTGKCCPANGC 60
           MVVAPAVFLL+ LQLVAVV+AAETPSGG  Q+EFDYFVLALQWPATSCKNTGKCCPANGC
Sbjct: 11  MVVAPAVFLLLALQLVAVVLAAETPSGGGGQKEFDYFVLALQWPATSCKNTGKCCPANGC 70

Query: 61  CRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLREDIEKYWPTYRCGTTSNC 120
           CRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLL EDIEKYWPTYRCGTTSNC
Sbjct: 71  CRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLSEDIEKYWPTYRCGTTSNC 130

Query: 121 HVWSVSSSLPIFLELDILFYSNSNPNPPFSEKHGTCAAPVIVGEYDYFLTTLTIFSKYN- 180
           H    S            F+++        EKHGTCAAPVIVGEYDYFLTTLTIFSKYN 
Sbjct: 131 HGTKGS------------FWAHEY------EKHGTCAAPVIVGEYDYFLTTLTIFSKYNV 190

Query: 181 -KALSDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFEPRDC 240
            KALSDAGFVASNTEKYPL DVVDAVKNATNSTPKISCAKKGAVKELWICFDK FEPRDC
Sbjct: 191 TKALSDAGFVASNTEKYPLGDVVDAVKNATNSTPKISCAKKGAVKELWICFDKNFEPRDC 250

Query: 241 DLANSCPEFIKLPTYEPQDVVEGAIPWIADAEELI 271
           DLANSCPEFIKLPTYEPQ VVEGAIPWIADAEELI
Sbjct: 251 DLANSCPEFIKLPTYEPQGVVEGAIPWIADAEELI 267

BLAST of Csor.00g245280 vs. ExPASy TrEMBL
Match: A0A0A0L0P9 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_4G109540 PE=3 SV=1)

HSP 1 Score: 407 bits (1045), Expect = 3.04e-141
Identity = 196/272 (72.06%), Postives = 220/272 (80.88%), Query Frame = 0

Query: 7   VFLLVLQLVAVVI--AAETPSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCRGADS 66
           V +++LQL+ VV   +AE PS  EQREFDYFVLALQWP +SCKN GKCCP+N CCRGA+S
Sbjct: 14  VVVVLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGKCCPSNACCRGAES 73

Query: 67  PTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLREDIEKYWPTYRCGTTSNCHVWSVS 126
           PTEFTIHGLWPQYNEKGWPSCCTDA FN+NEI++L EDI+KYWPTYRCGTTS CH    S
Sbjct: 74  PTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPTYRCGTTSTCHQTKGS 133

Query: 127 SSLPIFLELDILFYSNSNPNPPFSEKHGTCAAPVIVGEYDYFLTTLTIFSKYN--KALSD 186
                       F+++        EKHGTCAAPVIVGEYDYFLTT+TIFSKYN  K LSD
Sbjct: 134 ------------FWAHEY------EKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSD 193

Query: 187 AGFVASNTEKYPLEDVVDAVKNA-TNSTPKISCAKKGAVKELWICFDKKFEPRDCDLANS 246
           AGFVASNTEKYP+EDVV A+KN   N+TPKISCAKKGAVKELW+CFDK FEPRDC+L NS
Sbjct: 194 AGFVASNTEKYPIEDVVAAIKNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDCNLPNS 253

Query: 247 CPEFIKLPTYEPQDV--VEGAIPWIADAEELI 271
           CP+FIK PTYEPQ+   VEGAIPWIAD EE+I
Sbjct: 254 CPDFIKFPTYEPQEPNKVEGAIPWIADVEEII 267

BLAST of Csor.00g245280 vs. ExPASy TrEMBL
Match: A0A5A7V3T6 (Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold753G00520 PE=3 SV=1)

HSP 1 Score: 403 bits (1036), Expect = 6.40e-140
Identity = 195/275 (70.91%), Postives = 221/275 (80.36%), Query Frame = 0

Query: 5   PAVFL-LVLQLVAVVI--AAETPSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCRG 64
           P VFL L+++L+ VV   +AE PSG EQREFDYFVLALQWPATSCKN GKCCP+N CCRG
Sbjct: 10  PMVFLFLIVELLTVVFGFSAEAPSGSEQREFDYFVLALQWPATSCKNAGKCCPSNACCRG 69

Query: 65  ADSPTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLREDIEKYWPTYRCGTTSNCHVW 124
           ADSPTEFTIHGLWPQYNEKGWPSCCTDA FN+NEI +L EDI+KYWPTYRCGT+S CH  
Sbjct: 70  ADSPTEFTIHGLWPQYNEKGWPSCCTDASFNENEIGILAEDIQKYWPTYRCGTSSTCHRT 129

Query: 125 SVSSSLPIFLELDILFYSNSNPNPPFSEKHGTCAAPVIVGEYDYFLTTLTIFSKYN--KA 184
             S            F+++        EKHGTCAAPV+VGEYDYFLT LTIFSKYN  K 
Sbjct: 130 KGS------------FWAH--------EKHGTCAAPVLVGEYDYFLTALTIFSKYNVTKV 189

Query: 185 LSDAGFVASNTEKYPLEDVVDAVKNAT-NSTPKISCAKKGAVKELWICFDKKFEPRDCDL 244
           LS+ GFVASNTEKYP+EDV+ A+KN   N+TPKI+CAKKG VKELW+CFDK FEPRDC+L
Sbjct: 190 LSEGGFVASNTEKYPIEDVIAAIKNKFYNATPKITCAKKGVVKELWLCFDKSFEPRDCNL 249

Query: 245 ANSCPEFIKLPTYEPQDV--VEGAIPWIADAEELI 271
            +SCP+FIKLPTYEPQ+   VEGAIPWIAD EE+I
Sbjct: 250 QHSCPDFIKLPTYEPQEPNKVEGAIPWIADVEEII 264

BLAST of Csor.00g245280 vs. ExPASy TrEMBL
Match: A0A1S3CHX1 (Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103501148 PE=3 SV=1)

HSP 1 Score: 403 bits (1036), Expect = 6.87e-140
Identity = 195/275 (70.91%), Postives = 221/275 (80.36%), Query Frame = 0

Query: 5   PAVFL-LVLQLVAVVI--AAETPSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCRG 64
           P VFL L+++L+ VV   +AE PSG EQREFDYFVLALQWPATSCKN GKCCP+N CCRG
Sbjct: 10  PMVFLFLIVELLTVVFGFSAEAPSGSEQREFDYFVLALQWPATSCKNAGKCCPSNACCRG 69

Query: 65  ADSPTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLREDIEKYWPTYRCGTTSNCHVW 124
           ADSPTEFTIHGLWPQYNEKGWPSCCTDA FN+NEI +L EDI+KYWPTYRCGT+S CH  
Sbjct: 70  ADSPTEFTIHGLWPQYNEKGWPSCCTDASFNENEIGILAEDIQKYWPTYRCGTSSTCHRT 129

Query: 125 SVSSSLPIFLELDILFYSNSNPNPPFSEKHGTCAAPVIVGEYDYFLTTLTIFSKYN--KA 184
             S            F+++        EKHGTCAAPV+VGEYDYFLT LTIFSKYN  K 
Sbjct: 130 KGS------------FWAHEY------EKHGTCAAPVLVGEYDYFLTALTIFSKYNVTKV 189

Query: 185 LSDAGFVASNTEKYPLEDVVDAVKNAT-NSTPKISCAKKGAVKELWICFDKKFEPRDCDL 244
           LS+ GFVASNTEKYP+EDV+ A+KN   N+TPKI+CAKKG VKELW+CFDK FEPRDC+L
Sbjct: 190 LSEGGFVASNTEKYPIEDVIAAIKNKFYNATPKITCAKKGVVKELWLCFDKSFEPRDCNL 249

Query: 245 ANSCPEFIKLPTYEPQDV--VEGAIPWIADAEELI 271
            +SCP+FIKLPTYEPQ+   VEGAIPWIAD EE+I
Sbjct: 250 QHSCPDFIKLPTYEPQEPNKVEGAIPWIADVEEII 266

BLAST of Csor.00g245280 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 241.9 bits (616), Expect = 6.0e-64
Identity = 119/256 (46.48%), Postives = 161/256 (62.89%), Query Frame = 0

Query: 9   LLVLQLVAVVIAAET-PSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCRGADSPTE 68
           LL++  +A   A +       QREFDYF L+LQWP T C+ T  CC  N CCRG+D+PT+
Sbjct: 8   LLLVACIAGAFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQ 67

Query: 69  FTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLREDIEKYWPTYRCGTTSNCHVWSVSSSL 128
           FTIHGLWP YN+  WPSCC  + F + EIS L + +EKYWP+  CG+ S+C+    S   
Sbjct: 68  FTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGS--- 127

Query: 129 PIFLELDILFYSNSNPNPPFSEKHGTCAAPVIVGEYDYFLTTLTIFSKYN--KALSDAGF 188
                    F+ +        EKHGTC++PV   EY+YFLTTL ++ K+N    L  AG+
Sbjct: 128 ---------FWGHE------WEKHGTCSSPVFHDEYNYFLTTLNLYLKHNVTDVLYQAGY 187

Query: 189 VASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFEPRDC----DLAN- 248
           VASN+EKYPL  +V A++NA + TP++ C K+ A+ E+ ICF K F+PRDC    DL + 
Sbjct: 188 VASNSEKYPLGGIVTAIQNAFHITPEVVC-KRDAIDEIRICFYKDFKPRDCVGSQDLTSR 244

Query: 249 -SCPEFIKLPTYEPQD 256
            SCP+++ LP Y P D
Sbjct: 248 KSCPKYVSLPEYTPLD 244

BLAST of Csor.00g245280 vs. TAIR 10
Match: AT2G39780.2 (ribonuclease 2 )

HSP 1 Score: 196.1 bits (497), Expect = 3.8e-50
Identity = 101/254 (39.76%), Postives = 136/254 (53.54%), Query Frame = 0

Query: 9   LLVLQLVAVVIAAET-PSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCRGADSPTE 68
           LL++  +A   A +       QREFDYF L+LQWP T C+ T  CC  N CCRG+D+PT+
Sbjct: 8   LLLVACIAGAFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQ 67

Query: 69  FTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLREDIEKYWPTYRCGTTSNCHVWSVSSSL 128
           FTIHGLWP YN+  WPSCC  + F + EIS L + +EKYWP+  CG+ S+C+    S   
Sbjct: 68  FTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGS--- 127

Query: 129 PIFLELDILFYSNSNPNPPFSEKHGTCAAPVIVGEYDYFLTTLTIFSKYNKALSDAGFVA 188
                                                        F  +   L  AG+VA
Sbjct: 128 ---------------------------------------------FWGHEDVLYQAGYVA 187

Query: 189 SNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFEPRDC----DLAN--S 248
           SN+EKYPL  +V A++NA + TP++ C K+ A+ E+ ICF K F+PRDC    DL +  S
Sbjct: 188 SNSEKYPLGGIVTAIQNAFHITPEVVC-KRDAIDEIRICFYKDFKPRDCVGSQDLTSRKS 212

Query: 249 CPEFIKLPTYEPQD 256
           CP+++ LP Y P D
Sbjct: 248 CPKYVSLPEYTPLD 212

BLAST of Csor.00g245280 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 110.2 bits (274), Expect = 2.7e-24
Identity = 69/234 (29.49%), Postives = 107/234 (45.73%), Query Frame = 0

Query: 29  QREFDYFVLALQWPATSCKNTGKCC-PANGCCRGADSPTEFTIHGLWPQYNEKGWPS-CC 88
           Q +FD+F   LQWP   C  +  CC P +G         +F IHGLWP YN   WPS C 
Sbjct: 25  QEDFDFFYFVLQWPGAYCDTSRACCYPTSG-----KPAADFGIHGLWPNYNGGSWPSNCD 84

Query: 89  TDAVFNQNEISLLREDIEKYWPTYRCGTTSNCHVWSVSSSLPIFLELDILFYSNSNPNPP 148
            D+ F++++IS L   ++K WPT  C +    + W                         
Sbjct: 85  PDSQFDRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHE---------------------- 144

Query: 149 FSEKHGTCAAPVIVGEYDYFLTTLTIFSKYN--KALSDAGFVASNTEKYPLEDVVDAVKN 208
             EKHGTC+  V + +++YF   L +  K N  + L ++G +  +   Y L+ + +A+K+
Sbjct: 145 -WEKHGTCSESV-MDQHEYFENALKLKQKANLLQILKNSG-INPDDGFYNLDKITNAIKD 204

Query: 209 ATNSTPKISCAK----KGAVKELWICFDKK-FEPRDCDL--ANSCPEFIKLPTY 252
               TP I C K       + +++IC D    E  +C +    SCP  I+   +
Sbjct: 205 GIGFTPGIECNKDPERNAQLHQIYICVDTSGTEFIECPVLPRGSCPSQIQFSKF 228

BLAST of Csor.00g245280 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 105.1 bits (261), Expect = 8.7e-23
Identity = 67/227 (29.52%), Postives = 104/227 (45.81%), Query Frame = 0

Query: 10  LVLQLVAVVIAAETPSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCRGADSPTEFT 69
           L L  + V++ +   +     +FD+F    QWP + C    KCC  N     AD    F 
Sbjct: 8   LCLISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAAD----FG 67

Query: 70  IHGLWPQYNEKGWPS-CCTDAVFNQNEISLLREDIEKYWPTYRCGTTSNCHVWSVSSSLP 129
           IHGLWP Y +  +PS C     F+ + IS L   ++K WPT  C + S    W       
Sbjct: 68  IHGLWPNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHE---- 127

Query: 130 IFLELDILFYSNSNPNPPFSEKHGTCAAPVIVGEYDYFLTTLTIFSKYN--KALSDAGFV 189
                               EKHGTC+  VI  +++YF T L +  K N   AL+ AG +
Sbjct: 128 -------------------WEKHGTCSESVI-DQHEYFQTALNLKQKTNLLGALTKAG-I 187

Query: 190 ASNTEKYPLEDVVDAVKNATNSTPKISCAKKGA----VKELWICFDK 230
             + + Y LE + D++K +   TP + C + G+    + ++++C D+
Sbjct: 188 NPDGKSYSLESIRDSIKESIGFTPWVECNRDGSGNSQLYQVYLCVDR 205

BLAST of Csor.00g245280 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 102.8 bits (255), Expect = 4.3e-22
Identity = 66/230 (28.70%), Postives = 103/230 (44.78%), Query Frame = 0

Query: 7   VFLLVLQLVAVVIAAETPSGGEQREFDYFVLALQWPATSCKNTGKCC-PANGCCRGADSP 66
           +F+L LQ + V   A+        +FD+F   LQWP   C +   CC P  G        
Sbjct: 5   IFILALQQLYVQSFAQ--------DFDFFYFVLQWPGAYCDSRHSCCYPQTG-----KPA 64

Query: 67  TEFTIHGLWPQYNEKGWP-SCCTDAVFNQNEISLLREDIEKYWPTYRCGTTSNCHVWSVS 126
            +F IHGLWP Y   GWP +C  D+ F+   +S L  D+++ WPT  C +      W+  
Sbjct: 65  ADFGIHGLWPNYKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHE 124

Query: 127 SSLPIFLELDILFYSNSNPNPPFSEKHGTCAAPVIVGEYDYFLTTLTIFSKYN--KALSD 186
                                   EKHGTCA   +  ++DYF   L +  K N   AL++
Sbjct: 125 -----------------------WEKHGTCAESEL-DQHDYFEAGLKLKQKANLLHALTN 184

Query: 187 AGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGA----VKELWICFD 229
           AG +  + + Y ++D+ + +K      P I C K  +    + ++++C D
Sbjct: 185 AG-IKPDDKFYEMKDIENTIKQVVGFAPGIECNKDSSHNSQLYQIYLCVD 196

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P428148.4e-6346.48Ribonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=RNS2 PE=2 SV=1[more]
P428131.2e-2129.52Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1[more]
P428156.1e-2128.70Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1[more]
P801961.0e-2026.89Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2[more]
P800225.2e-2026.27Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
KAG6576854.17.50e-207100.00Ribonuclease 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022922895.11.47e-17387.55ribonuclease 2-like [Cucurbita moschata][more]
XP_022984766.12.30e-16685.82ribonuclease 2-like [Cucurbita maxima][more]
XP_023553229.12.04e-16483.52ribonuclease 2-like [Cucurbita pepo subsp. pepo][more]
XP_038876700.14.17e-14674.82ribonuclease 2-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1EA397.11e-17487.55Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111430737 PE=3 SV=1[more]
A0A6J1J6701.11e-16685.82Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111482949 PE=3 SV=1[more]
A0A0A0L0P93.04e-14172.06Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_4G109540 PE=3 SV=1[more]
A0A5A7V3T66.40e-14070.91Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold753G00... [more]
A0A1S3CHX16.87e-14070.91Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103501148 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G39780.16.0e-6446.48ribonuclease 2 [more]
AT2G39780.23.8e-5039.76ribonuclease 2 [more]
AT1G14220.12.7e-2429.49Ribonuclease T2 family protein [more]
AT2G02990.18.7e-2329.52ribonuclease 1 [more]
AT1G26820.14.3e-2228.70ribonuclease 3 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001568Ribonuclease T2-likePFAMPF00445Ribonuclease_T2coord: 32..122
e-value: 6.5E-16
score: 59.0
coord: 149..236
e-value: 1.5E-18
score: 67.6
IPR001568Ribonuclease T2-likePANTHERPTHR11240RIBONUCLEASE T2coord: 149..258
IPR001568Ribonuclease T2-likePANTHERPTHR11240RIBONUCLEASE T2coord: 23..115
IPR036430Ribonuclease T2-like superfamilyGENE3D3.90.730.10coord: 28..253
e-value: 2.0E-61
score: 209.6
IPR036430Ribonuclease T2-like superfamilySUPERFAMILY55895Ribonuclease Rh-likecoord: 31..243
NoneNo IPR availablePANTHERPTHR11240:SF66OS01G0897300 PROTEINcoord: 23..115
NoneNo IPR availablePANTHERPTHR11240:SF66OS01G0897300 PROTEINcoord: 149..258
IPR018188Ribonuclease T2, His active site 1PROSITEPS00530RNASE_T2_1coord: 68..75
IPR033697Ribonuclease T2, eukaryoticCDDcd01061RNase_T2_eukcoord: 32..243
e-value: 2.54284E-50
score: 162.116

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g245280.m01Csor.00g245280.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006401 RNA catabolic process
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component GO:0005576 extracellular region
molecular_function GO:0033897 ribonuclease T2 activity
molecular_function GO:0003723 RNA binding