Csor.00g244680 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g244680
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	trypsin inhibitor 1-like
Location	Csor_Chr16: 1543326 .. 1545223 (+)
RNA-Seq Expression	Csor.00g244680
Synteny	Csor.00g244680

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintronterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTGGAAAAGAGGTGCTTTAGTTGCAATGGTGGGGCTCTTGCTGATAGCCGCTTTTGCAGAGTCCACCGCCGTCGACGCCGCCGTGGATGAAGTGATTCAACTAGTTTCCGATGGAGGGAATGATTTCCCAAGAAAGATGATGAAGGGCGCCGAAGAGTGTCCTAGACAACTTGTTAGGTGCAAGAAGGATTCCGACTGCCATCGTGCTTGCAAATGCATGCCGTACCACTTCTGTGGTTAACTCGGCTGACTCCGACGAAGAAGCTCCGCCGTGTCGGATGTTCTACAATAATGTCGTTCCTTATTTCCCTCCATGTCTGTCTCTCTGTCTTTTTCTAATTATGTGAAAAATAAAAGTCCGTATGTCTCTTTCATCACTCTCTCGTCTGCGAGTGCATGTGATCAATTCGTGCATATGACGTTCGTCTACCATTTTGTTGTAATATTTTCTATGTCCTTATGAATCTATCTTCTTTTCGTTTTAAACTTTGGTATAATTAAATTTATCACGATGAATCGTTTAAATCTTTCGATTCTCAAATTATTCAACATGATATCAAAGTGGAGTTGTAACGGCCCAAGCCCTCCTTGCTAACGAACTAACACTCGGTTAAAGAGTGGAACAAAATATGTTTTAAAACCGTGAGGTTGCGGATGATACGTAATGGGCTAAAGCGAACTATATCTACTGGTAGTGGACTTGAGAACACTCGGTTGGAGAGTGGAACAAAGTATTTCTTATAAGGGTGTGGAAACATCTATCTACTAAGTGTGTTTTAAAACCACGAGGTTGAAAATGATGCGTAATGGGTTAAAGCAAACTATATGTACTAGCAGTGACTTGAGTGTTATAAATGGTATCAGAGCTAGATACTAGGTGGTGTGCTAGCGTGGACGCTGCCTTTAAGGGATGGATTGTGAGATCCCGCATGGGTTAGAGAATTGAATGAAACGTTCCTTAAAGACATAGAAACCTCTCCTAACATACGCGTTTTAAAACTTTGAGAAGAAGTTTGAAAAGAAAATTCCAAATAAGACCATATAAGCTCGAAGGATTATTTTGCAATTCGGTAATAATTCCCTCCAAATGAGTGGTTCATTATCGGGCTATACAATATCACAATGGATATATATATTTTTAATTTATTTAAAGAAAATATTTTATAGCAATTTCGTTTTCAAGCACCCTAACAAATGGCCACGTATGTCCTCGTTGGACCATTTCCCCGTTCATTGTCATCATGGTTGTCCTCTCTATGTACTATGAGACGAATTTATCAAATCTCAGCCACACAAATAGTATAGAGATCCCACTTAGTTAGATAGAAGAACGAAATATTCTTTATAAAAGTGTAGAAATGTATCTCTAGTAGACGCGTTTTAAAAACTTTGAGGAAAAGCTCAGAAGAGAAAGCCAAAAATGGACAATATCGGCTAGCGGTGGACTTCATGGTGGACTTCAGTTGTTATATACCCACTTGATGCTTTGCACTAGCTCACCATCGTCTAAGCAGAAAACAACGACACATCGATGGTATCCAGGATAGATTTGAATCCTGAGATTATAAGTAAGAAACACTATCGATAGTTTATAAATAAGGAAGTTCTGTATAGAGACGCTCGAGAGAGTTCGACGGAATATGGCATGGAACGGTAGTATGTACGGTTTAGAGCCAAGACATTTATAGGTCTCCTATCGGAGAGAAAATAGGCAAGAAAGAGAGAGAAAGAATGGAGTGGAAACGAGCTGGTTTAGCTGCAATGGTGGGGATGTTGCTGATGGCTGCTTTTACAGAGTCGGGGGACGTCGAGGTCGAGGTGGATGAAATGATTGAACTAGTTAGCGATAGAGGAGTGAATGATTTGGATGAAGCGTCCTCCAAGATGCCCTAG

mRNA sequence

ATGGAGTGGAAAAGAGGTGCTTTAGTTGCAATGGTGGGGCTCTTGCTGATAGCCGCTTTTGCAGAGTCCACCGCCGTCGACGCCGCCGTGGATGAAGTGATTCAACTAGTTTCCGATGGAGGGAATGATTTCCCAAGAAAGATGATGAAGGGCGCCGAAGAGTGTCCTAGACAACTTGTTAGGTGCAAGAAGGATTCCGACTGCCATCGTGCTTGCAAATGCATGCCGTACCACTTCTGTGGCAAGAAAGAGAGAGAAAGAATGGAGTGGAAACGAGCTGGTTTAGCTGCAATGGTGGGGATGTTGCTGATGGCTGCTTTTACAGAGTCGGGGGACGTCGAGGTCGAGGTGGATGAAATGATTGAACTAGTTAGCGATAGAGGAGTGAATGATTTGGATGAAGCGTCCTCCAAGATGCCCTAG

Coding sequence (CDS)

ATGGAGTGGAAAAGAGGTGCTTTAGTTGCAATGGTGGGGCTCTTGCTGATAGCCGCTTTTGCAGAGTCCACCGCCGTCGACGCCGCCGTGGATGAAGTGATTCAACTAGTTTCCGATGGAGGGAATGATTTCCCAAGAAAGATGATGAAGGGCGCCGAAGAGTGTCCTAGACAACTTGTTAGGTGCAAGAAGGATTCCGACTGCCATCGTGCTTGCAAATGCATGCCGTACCACTTCTGTGGCAAGAAAGAGAGAGAAAGAATGGAGTGGAAACGAGCTGGTTTAGCTGCAATGGTGGGGATGTTGCTGATGGCTGCTTTTACAGAGTCGGGGGACGTCGAGGTCGAGGTGGATGAAATGATTGAACTAGTTAGCGATAGAGGAGTGAATGATTTGGATGAAGCGTCCTCCAAGATGCCCTAG

Protein sequence

MEWKRGALVAMVGLLLIAAFAESTAVDAAVDEVIQLVSDGGNDFPRKMMKGAEECPRQLVRCKKDSDCHRACKCMPYHFCGKKERERMEWKRAGLAAMVGMLLMAAFTESGDVEVEVDEMIELVSDRGVNDLDEASSKMP

Homology

[Close]

BLAST of Csor.00g244680 vs. ExPASy Swiss-Prot
Match: Q43667 (Trypsin inhibitor 1 OS=Trichosanthes kirilowii OX=3677 PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 5.4e-13
Identity = 35/65 (53.85%), Postives = 43/65 (66.15%), Query Frame = 0


Query: 17 IAAFAESTAVDAAVDEVIQLVSDGGNDFPRKMMKGAEECPRQLVRCKKDSDCHRACKCMP 76
          +AAF ES    A  DEVIQLVSDG N++  KMM+G   CPR L+ CK + DC R CKC+ 
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKMMEGVVACPRILMPCKVNDDCLRGCKCLS 60

Query: 77 YHFCG 82
            +CG
Sbjct: 61 NGYCG 65

[Close]

BLAST of Csor.00g244680 vs. ExPASy Swiss-Prot
Match: P34950 (Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 5.6e-10
Identity = 31/61 (50.82%), Postives = 39/61 (63.93%), Query Frame = 0


Query: 23 STAVDAAVDEVIQLVSDGGNDFPRKMMKGAEE--CPRQLVRCKKDSDCHRACKCMPYHFC 82
          S A  + V EVI+L+SDGGND PRK+M G     CPR L+ CK D DC   C+C+   +C
Sbjct: 3  SVAESSGVVEVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCLSNGYC 62

[Close]

BLAST of Csor.00g244680 vs. NCBI nr
Match: KAG6576914.1 (hypothetical protein SDJN03_24488, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 276 bits (705), Expect = 2.47e-93
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 0


Query: 1   MEWKRGALVAMVGLLLIAAFAESTAVDAAVDEVIQLVSDGGNDFPRKMMKGAEECPRQLV 60
           MEWKRGALVAMVGLLLIAAFAESTAVDAAVDEVIQLVSDGGNDFPRKMMKGAEECPRQLV
Sbjct: 1   MEWKRGALVAMVGLLLIAAFAESTAVDAAVDEVIQLVSDGGNDFPRKMMKGAEECPRQLV 60

Query: 61  RCKKDSDCHRACKCMPYHFCGKKERERMEWKRAGLAAMVGMLLMAAFTESGDVEVEVDEM 120
           RCKKDSDCHRACKCMPYHFCGKKERERMEWKRAGLAAMVGMLLMAAFTESGDVEVEVDEM
Sbjct: 61  RCKKDSDCHRACKCMPYHFCGKKERERMEWKRAGLAAMVGMLLMAAFTESGDVEVEVDEM 120

Query: 121 IELVSDRGVNDLDEASSKMP 140
           IELVSDRGVNDLDEASSKMP
Sbjct: 121 IELVSDRGVNDLDEASSKMP 140

[Close]

BLAST of Csor.00g244680 vs. NCBI nr
Match: XP_023552845.1 (uncharacterized protein LOC111810370 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 190 bits (483), Expect = 3.58e-59
Identity = 106/132 (80.30%), Postives = 112/132 (84.85%), Query Frame = 0


Query: 1   MEWKRGALVAMVGLLLIAAFAESTAVDAAVDEVIQLVSDGGNDFPRKMMKGAEECPRQLV 60
           ME KRGALVAMVGLLLIAAFAEST    AVDEVIQLVSDGGNDFPRKMMKGAEECPRQLV
Sbjct: 1   MERKRGALVAMVGLLLIAAFAEST----AVDEVIQLVSDGGNDFPRKMMKGAEECPRQLV 60

Query: 61  RCKKDSDCHRACKCMPYHFCG-KKERERMEWKRAGLAAMVGMLLMAAFTESGDVEVEVDE 120
           RCKKDSDCHRACKCMPYHFCG   +RE+MEWKR  L AMVG+LLMAAF +S  V     E
Sbjct: 61  RCKKDSDCHRACKCMPYHFCGWATKREKMEWKRGALIAMVGLLLMAAFADSAAVH--GGE 120

Query: 121 MIELVSDRGVND 131
           +I+LVSD GVND
Sbjct: 121 VIQLVSD-GVND 125

[Close]

BLAST of Csor.00g244680 vs. NCBI nr
Match: KAG6576915.1 (hypothetical protein SDJN03_24489, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 141 bits (356), Expect = 6.64e-40
Identity = 83/126 (65.87%), Postives = 92/126 (73.02%), Query Frame = 0


Query: 11  MVGLLLIAAFAESTAVDAAVDEVIQLVSDGGNDFPRKMMKGAEECPRQLVRCKKDSDCHR 70
           MVG LL+AAFAES AVDAAVDEVIQLVSDGGNDFPRKMM GA+ CPR L+ CK DSDC  
Sbjct: 1   MVGFLLVAAFAESAAVDAAVDEVIQLVSDGGNDFPRKMMNGAQGCPRILMPCKVDSDCFP 60

Query: 71  ACKCMPYHFCGKK-----ERERMEWKRAGLAAMVGMLLMAAFTESGDVEVEVDEMIELVS 130
            CKC    +   K     +RE+MEWKR  L AMVG+LLMAAF ES  V     E+I+LVS
Sbjct: 61  TCKCRSNRYERVKGIMGNKREKMEWKRGALIAMVGLLLMAAFAESAAVH--GGEVIQLVS 120

[Close]

BLAST of Csor.00g244680 vs. NCBI nr
Match: KAG7014942.1 (hypothetical protein SDJN02_22573, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 140 bits (354), Expect = 1.34e-39
Identity = 83/126 (65.87%), Postives = 92/126 (73.02%), Query Frame = 0


Query: 11  MVGLLLIAAFAESTAVDAAVDEVIQLVSDGGNDFPRKMMKGAEECPRQLVRCKKDSDCHR 70
           MVG LL+AAFAES AVDAAVDEVIQLVSDGGNDFPRKMM GA+ CPR L+ CK DSDC  
Sbjct: 1   MVGFLLVAAFAESAAVDAAVDEVIQLVSDGGNDFPRKMMNGAQGCPRILMPCKVDSDCFP 60

Query: 71  ACKCMPYHFCGKK-----ERERMEWKRAGLAAMVGMLLMAAFTESGDVEVEVDEMIELVS 130
            CKC    +   K     +RE+MEWKR  L AMVG+LLMAAF ES  V     E+I+LVS
Sbjct: 61  TCKCRSNGYERVKGIMGNKREKMEWKRGALIAMVGLLLMAAFAESAAVH--GGEVIQLVS 120

[Close]

BLAST of Csor.00g244680 vs. NCBI nr
Match: XP_022984819.1 (trypsin inhibitor 1-like [Cucurbita maxima])

HSP 1 Score: 105 bits (261), Expect = 1.54e-26
Identity = 56/81 (69.14%), Postives = 61/81 (75.31%), Query Frame = 0


Query: 1  MEWKRGALVAMVGLLLIAAFAESTAVDAAVDEVIQLVSDGGNDFPRKMMKGAEECPRQLV 60
          MEWKRGAL+AMVGLLL+AAFAES AV     EVIQLVSDG NDFPRKMM G + CPR L+
Sbjct: 1  MEWKRGALIAMVGLLLMAAFAESAAVHGG--EVIQLVSDGVNDFPRKMMNGVQGCPRILM 60

Query: 61 RCKKDSDCHRACKCMPYHFCG 81
           C+ DSDC   C C P  FCG
Sbjct: 61 ECELDSDCLPGCICRPNGFCG 79

[Close]

BLAST of Csor.00g244680 vs. ExPASy TrEMBL
Match: A0A6J1JBL8 (trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482991 PE=3 SV=1)

HSP 1 Score: 105 bits (261), Expect = 7.45e-27
Identity = 56/81 (69.14%), Postives = 61/81 (75.31%), Query Frame = 0


Query: 1  MEWKRGALVAMVGLLLIAAFAESTAVDAAVDEVIQLVSDGGNDFPRKMMKGAEECPRQLV 60
          MEWKRGAL+AMVGLLL+AAFAES AV     EVIQLVSDG NDFPRKMM G + CPR L+
Sbjct: 1  MEWKRGALIAMVGLLLMAAFAESAAVHGG--EVIQLVSDGVNDFPRKMMNGVQGCPRILM 60

Query: 61 RCKKDSDCHRACKCMPYHFCG 81
           C+ DSDC   C C P  FCG
Sbjct: 61 ECELDSDCLPGCICRPNGFCG 79

[Close]

BLAST of Csor.00g244680 vs. ExPASy TrEMBL
Match: A0A0A0KY34 (Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 2.19e-16
Identity = 45/83 (54.22%), Postives = 58/83 (69.88%), Query Frame = 0


Query: 1  MEWKRGALVAMVGLLLIAAFAESTAVDAAVDE-VIQLVSDGGNDFPRKMMKG-AEECPRQ 60
          MEWK+ ALVAM+G+LL+A F ES  V   VDE +IQLVSDG N++ + + K  A  CPR 
Sbjct: 1  MEWKKIALVAMMGMLLMATFTES--VGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRI 60

Query: 61 LVRCKKDSDCHRACKCMPYHFCG 81
          L++CK DSDC+  C C P  +CG
Sbjct: 61 LMKCKTDSDCYPGCTCKPMGYCG 81

[Close]

BLAST of Csor.00g244680 vs. ExPASy TrEMBL
Match: A0A4P2SJP1 (Trypsin inhibitor I1 OS=Trichosanthes kirilowii OX=3677 PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 1.32e-14
Identity = 36/64 (56.25%), Postives = 42/64 (65.62%), Query Frame = 0


Query: 18 AAFAESTAVDAAVDEVIQLVSDGGNDFPRKMMKGAEECPRQLVRCKKDSDCHRACKCMPY 77
          AAF ES    A  DEVIQLVSDG N++  KMMKG   CPR L+ CK + DC R CKC+  
Sbjct: 2  AAFVESARAGAGADEVIQLVSDGVNEYSEKMMKGVVGCPRILMPCKVNDDCLRGCKCLSN 61

Query: 78 HFCG 81
           +CG
Sbjct: 62 GYCG 65

[Close]

BLAST of Csor.00g244680 vs. ExPASy TrEMBL
Match: A0A4P2SLY2 (Trypsin inhibitor I2 OS=Trichosanthes kirilowii OX=3677 PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 3.73e-14
Identity = 35/64 (54.69%), Postives = 42/64 (65.62%), Query Frame = 0


Query: 18 AAFAESTAVDAAVDEVIQLVSDGGNDFPRKMMKGAEECPRQLVRCKKDSDCHRACKCMPY 77
          AAF ES    A  DEVIQLVSDG N++  KMM+G   CPR L+ CK + DC R CKC+  
Sbjct: 2  AAFVESARAGAGADEVIQLVSDGVNEYSEKMMEGVVGCPRVLMPCKVNDDCLRGCKCLSN 61

Query: 78 HFCG 81
           +CG
Sbjct: 62 GYCG 65

[Close]

BLAST of Csor.00g244680 vs. ExPASy TrEMBL
Match: A0A5D3E0W0 (Trypsin inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00600 PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.54e-12
Identity = 42/82 (51.22%), Postives = 52/82 (63.41%), Query Frame = 0


Query: 1  MEWKRGALVAMVGLLLIAAFAESTAVDAAVDE-VIQLVSDGGNDFPRKMMKGAEECPRQL 60
          MEWK+ ALVAM+G+LL+A F ES  V   VDE +IQLVSDG N++      G   CPR L
Sbjct: 1  MEWKKIALVAMMGMLLMATFTES--VGFGVDEEIIQLVSDGVNEY-----SGHPGCPRIL 60

Query: 61 VRCKKDSDCHRACKCMPYHFCG 81
          ++CK D DC   C C    +CG
Sbjct: 61 MKCKTDRDCLAGCTCKRNGYCG 75

The following BLAST results are available for this feature:

BLAST of Csor.00g244680 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q43667	5.4e-13	53.85	Trypsin inhibitor 1 OS=Trichosanthes kirilowii OX=3677 PE=3 SV=1	[more]
P34950	5.6e-10	50.82	Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]

BLAST of Csor.00g244680 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6576914.1	2.47e-93	100.00	hypothetical protein SDJN03_24488, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023552845.1	3.58e-59	80.30	uncharacterized protein LOC111810370 [Cucurbita pepo subsp. pepo]	[more]
KAG6576915.1	6.64e-40	65.87	hypothetical protein SDJN03_24489, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7014942.1	1.34e-39	65.87	hypothetical protein SDJN02_22573, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022984819.1	1.54e-26	69.14	trypsin inhibitor 1-like [Cucurbita maxima]	[more]

BLAST of Csor.00g244680 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JBL8	7.45e-27	69.14	trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482991 PE=3 SV=1	[more]
A0A0A0KY34	2.19e-16	54.22	Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1	[more]
A0A4P2SJP1	1.32e-14	56.25	Trypsin inhibitor I1 OS=Trichosanthes kirilowii OX=3677 PE=2 SV=1	[more]
A0A4P2SLY2	3.73e-14	54.69	Trypsin inhibitor I2 OS=Trichosanthes kirilowii OX=3677 PE=2 SV=1	[more]
A0A5D3E0W0	1.54e-12	51.22	Trypsin inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G...	[more]

BLAST of Csor.00g244680 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00286	PTI_1	coord: 53..81 e-value: 3.6E-5 score: 33.2
None	No IPR available	GENE3D	4.10.75.20		coord: 29..81 e-value: 1.6E-13 score: 52.3
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 55..81 e-value: 6.6E-7 score: 29.2
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 55..74
IPR000737	Proteinase inhibitor I7, squash	CDD	cd00150	PlantTI	coord: 55..81 e-value: 3.96978E-8 score: 44.6759
IPR011052	Proteinase/amylase inhibitor domain superfamily	SUPERFAMILY	57027	Plant inhibitors of proteinases and amylases	coord: 55..81

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g244680.m01	Csor.00g244680.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
cellular_component	GO:0110165	cellular anatomical entity
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity