Csor.00g244250 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g244250
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionVID27 domain-containing protein
LocationCsor_Chr16: 1742975 .. 1745237 (+)
RNA-Seq ExpressionCsor.00g244250
SyntenyCsor.00g244250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintronterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTACTGCGCAGAGCCGCGAGGACGCTGAACTTTCGGACTCCGATGAATACAAGGAAGAAGAGGAAGAGGATGATGAAGAAGAAGAATACGAAGACGCTGATGAAGTATTGAAGCCGCAATCGATCTCTAGCGCCAAATCGACCGGTACAGCATCTGCAGTTGATGATGTCGACGCCAAACTCAAAGCTCTTAAGCTCAAGTATGGCTCCTCTTCCCCTTCTCAGATACCCAATGCCAACAACGCTGTGAAACTTTACCTTCACATCGGTGGAAACACCCCCCGTGCAAAGTGGATCGTCTCTGAGAAACTCACTTTCTACGCTTTTCTCAAGACCTCGAATGTTGACGGAGATAGCGACGACGATCAAGAAGACGAAGAGGACGGTTATAAAGCCAATTCGTCGGGTGGGAGAGGCCAGTTACGTTGGATACTCAAAGTGGGGGGAAAGGTCAGAGCTCTGGTTTCGACAGAAATGCAATTGAAGATGTTTGGTGATCAGCGGCGTGTCGATTTTGTTAACAAAGGTGTGTGGGCTTTGAAGTTCCCGACGGATGAACAGTACCGGGACTTCGTGACCAAATTCCAAGATTACTTGTTTGAGAATGTTTATGGGCTTGAAGCGACCGAGGAGAACAAGGTCAAGATTTACGGGAAGGAGTTTATTGGGTGGTTGAAGCCAGAGGTGGCAGATGATTCGATTTGGGAAAACGCCGATATTGAGTTCGGGAAGAGCCCCAGCTCCTCAGTGAGGACGAAACAGGACTTGATAGAAGAGTTCGAGGAGGCTGCTAATGGAGGCGTACAGAGCTTGACATTAGGGGCATTGGATAACAGCTTCTTGGTGAACGACTCAGGGGTTCAGGTTTATAGGAATCTAAGCCATGGAATTCATGGGAAGGGGATTTCGTTGAAGTTTGGTGCAGGAAACTCACCGAACTCTAGTCGATCGACACCGAAGAAGGGTCTTCTCATGAAGGCTGAGACGAACATGCTTCTCATGAGTCCATTGAAGGAAGGAAAACCCCACGCATCAGGGCTGCAGCAGCTTGATATAGAGACGGGGAAAGTTGTTACAGAATGGAAGTTTGAAAAGGATGGTACAGATATTACAATGAGGGACATCACGAACGACACCAAAGGGTCACAATTGGATCCTTCAGAATCCACATTTTTGGGGTTGGACGACAATAGGCTTTGTCAATGGGACATGAGAGATCGTAGAGGCATGGTTCAGAACATTGGTGGAGCTGCTGATAATTCACCGGTTTTGAACTGGGCGCAGGGGCATCAGTTTTCAAGAGGGACAAACTTCCAATGCTTTGCTACAACAGGGGATGGGTCGATTGTTGTTGGGTCAGTTGATGGGAAGATAAGGCTGTATTCGAAGACATCGATGAGACAGGCGAAGACGGCCTTTCCCGGGCTCGGTTCGCCTATTACTCATGTCGATGTTACTCACGATGGAAAGTGGATTCTGGGGACGACTGATACTTATTTGATACTTATCTGCGTGTTATTTGTCGATAAAGATGGCAACACGAAGACTGGGTTCAGTGGTCGTATGGGCAACAAGATTCCCGCTCCGAGGTTGCTGAAGTTGACTCCTCTGGATTCCCATTTGGCTGGAACCGACAACAGATTCCATGGTGGCCACTTCTCTTGGGTAAGAAAACTTGTTCTGACTTTGAATGCTTGAATCTTGCTCTGTTTTGCGCTGTCTTGGTTGGTTTTCTTGTCCTTGACAGTCGTGATATCAATTTCTTAGTAACTTAGAGAAGTAAGGGGGTAGAAGAATCGGTGTCATTGAAGATCCATGACTAATGTTTTGGTTTATGTTCGTGTTATGGTGGTTATTCTATCATAGCTTCTGTTGTTCTTACAGGGAACTTCCCTCGTTTGAGCTTTCTTTCTTTTGCCACTTTAGACTGCAAGGTTCATACACAAACTTACGATCTTTTATCGACATTGAATTTCAGGTCACCGAGAGCGGGAAGCAAGAGCATCACCTCGTCGCAACGGTGGGAAAGTTCAGTGTGATATGGGACTTCCATCAGGTAAAGAACAGTGCACATGACTGCTACCGGAATCAGCAAGGACTCAAGAGCTGTTACTGCTACAAGATTGTGCTGAAAGATGAATCCATCGTCGAGAGCCGATTCATGCACGAAAAGTTTGCAGTCTCGGATTCTCCAGAAGCCCCACTGGTTGTGGCCACCCCCATGAAAGTCAGCTCCATCAGCCTATCTGGAAAGAGATGA

mRNA sequence

ATGGGTACTGCGCAGAGCCGCGAGGACGCTGAACTTTCGGACTCCGATGAATACAAGGAAGAAGAGGAAGAGGATGATGAAGAAGAAGAATACGAAGACGCTGATGAAGTATTGAAGCCGCAATCGATCTCTAGCGCCAAATCGACCGGTACAGCATCTGCAGTTGATGATGTCGACGCCAAACTCAAAGCTCTTAAGCTCAAGTATGGCTCCTCTTCCCCTTCTCAGATACCCAATGCCAACAACGCTGTGAAACTTTACCTTCACATCGGTGGAAACACCCCCCGTGCAAAGTGGATCGTCTCTGAGAAACTCACTTTCTACGCTTTTCTCAAGACCTCGAATGTTGACGGAGATAGCGACGACGATCAAGAAGACGAAGAGGACGGTTATAAAGCCAATTCGTCGGGTGGGAGAGGCCAGTTACGTTGGATACTCAAAGTGGGGGGAAAGGTCAGAGCTCTGGTTTCGACAGAAATGCAATTGAAGATGTTTGGTGATCAGCGGCGTGTCGATTTTGTTAACAAAGGTGTGTGGGCTTTGAAGTTCCCGACGGATGAACAGTACCGGGACTTCGTGACCAAATTCCAAGATTACTTGTTTGAGAATGTTTATGGGCTTGAAGCGACCGAGGAGAACAAGGTCAAGATTTACGGGAAGGAGTTTATTGGGTGGTTGAAGCCAGAGGTGGCAGATGATTCGATTTGGGAAAACGCCGATATTGAGTTCGGGAAGAGCCCCAGCTCCTCAGTGAGGACGAAACAGGACTTGATAGAAGAGTTCGAGGAGGCTGCTAATGGAGGCGTACAGAGCTTGACATTAGGGGCATTGGATAACAGCTTCTTGGTGAACGACTCAGGGGTTCAGGTTTATAGGAATCTAAGCCATGGAATTCATGGGAAGGGGATTTCGTTGAAGTTTGGTGCAGGAAACTCACCGAACTCTAGTCGATCGACACCGAAGAAGGGTCTTCTCATGAAGGCTGAGACGAACATGCTTCTCATGAGTCCATTGAAGGAAGGAAAACCCCACGCATCAGGGCTGCAGCAGCTTGATATAGAGACGGGGAAAGTTGTTACAGAATGGAAGTTTGAAAAGGATGGTACAGATATTACAATGAGGGACATCACGAACGACACCAAAGGGTCACAATTGGATCCTTCAGAATCCACATTTTTGGGGTTGGACGACAATAGGCTTTGTCAATGGGACATGAGAGATCGTAGAGGCATGGTTCAGAACATTGGTGGAGCTGCTGATAATTCACCGGTTTTGAACTGGGCGCAGGGGCATCAGTTTTCAAGAGGGACAAACTTCCAATGCTTTGCTACAACAGGGGATGGGTCGATTGTTGTTGGGTCAGTTGATGGGAAGATAAGGCTGTATTCGAAGACATCGATGAGACAGGCGAAGACGGCCTTTCCCGGGCTCGGTTCGCCTATTACTCATGTCGATGTTACTCACGATGGAAAGTGGATTCTGGGGACGACTGATACTTATTTGATACTTATCTGCGTGTTATTTGTCGATAAAGATGGCAACACGAAGACTGGGTTCAGTGGTCGTATGGGCAACAAGATTCCCGCTCCGAGGTTGCTGAAGTTGACTCCTCTGGATTCCCATTTGGCTGGAACCGACAACAGATTCCATGGTGGCCACTTCTCTTGGGTCACCGAGAGCGGGAAGCAAGAGCATCACCTCGTCGCAACGGTGGGAAAGTTCAGTGTGATATGGGACTTCCATCAGGTAAAGAACAGTGCACATGACTGCTACCGGAATCAGCAAGGACTCAAGAGCTGTTACTGCTACAAGATTGTGCTGAAAGATGAATCCATCGTCGAGAGCCGATTCATGCACGAAAAGTTTGCAGTCTCGGATTCTCCAGAAGCCCCACTGGTTGTGGCCACCCCCATGAAAGTCAGCTCCATCAGCCTATCTGGAAAGAGATGA

Coding sequence (CDS)

ATGGGTACTGCGCAGAGCCGCGAGGACGCTGAACTTTCGGACTCCGATGAATACAAGGAAGAAGAGGAAGAGGATGATGAAGAAGAAGAATACGAAGACGCTGATGAAGTATTGAAGCCGCAATCGATCTCTAGCGCCAAATCGACCGGTACAGCATCTGCAGTTGATGATGTCGACGCCAAACTCAAAGCTCTTAAGCTCAAGTATGGCTCCTCTTCCCCTTCTCAGATACCCAATGCCAACAACGCTGTGAAACTTTACCTTCACATCGGTGGAAACACCCCCCGTGCAAAGTGGATCGTCTCTGAGAAACTCACTTTCTACGCTTTTCTCAAGACCTCGAATGTTGACGGAGATAGCGACGACGATCAAGAAGACGAAGAGGACGGTTATAAAGCCAATTCGTCGGGTGGGAGAGGCCAGTTACGTTGGATACTCAAAGTGGGGGGAAAGGTCAGAGCTCTGGTTTCGACAGAAATGCAATTGAAGATGTTTGGTGATCAGCGGCGTGTCGATTTTGTTAACAAAGGTGTGTGGGCTTTGAAGTTCCCGACGGATGAACAGTACCGGGACTTCGTGACCAAATTCCAAGATTACTTGTTTGAGAATGTTTATGGGCTTGAAGCGACCGAGGAGAACAAGGTCAAGATTTACGGGAAGGAGTTTATTGGGTGGTTGAAGCCAGAGGTGGCAGATGATTCGATTTGGGAAAACGCCGATATTGAGTTCGGGAAGAGCCCCAGCTCCTCAGTGAGGACGAAACAGGACTTGATAGAAGAGTTCGAGGAGGCTGCTAATGGAGGCGTACAGAGCTTGACATTAGGGGCATTGGATAACAGCTTCTTGGTGAACGACTCAGGGGTTCAGGTTTATAGGAATCTAAGCCATGGAATTCATGGGAAGGGGATTTCGTTGAAGTTTGGTGCAGGAAACTCACCGAACTCTAGTCGATCGACACCGAAGAAGGGTCTTCTCATGAAGGCTGAGACGAACATGCTTCTCATGAGTCCATTGAAGGAAGGAAAACCCCACGCATCAGGGCTGCAGCAGCTTGATATAGAGACGGGGAAAGTTGTTACAGAATGGAAGTTTGAAAAGGATGGTACAGATATTACAATGAGGGACATCACGAACGACACCAAAGGGTCACAATTGGATCCTTCAGAATCCACATTTTTGGGGTTGGACGACAATAGGCTTTGTCAATGGGACATGAGAGATCGTAGAGGCATGGTTCAGAACATTGGTGGAGCTGCTGATAATTCACCGGTTTTGAACTGGGCGCAGGGGCATCAGTTTTCAAGAGGGACAAACTTCCAATGCTTTGCTACAACAGGGGATGGGTCGATTGTTGTTGGGTCAGTTGATGGGAAGATAAGGCTGTATTCGAAGACATCGATGAGACAGGCGAAGACGGCCTTTCCCGGGCTCGGTTCGCCTATTACTCATGTCGATGTTACTCACGATGGAAAGTGGATTCTGGGGACGACTGATACTTATTTGATACTTATCTGCGTGTTATTTGTCGATAAAGATGGCAACACGAAGACTGGGTTCAGTGGTCGTATGGGCAACAAGATTCCCGCTCCGAGGTTGCTGAAGTTGACTCCTCTGGATTCCCATTTGGCTGGAACCGACAACAGATTCCATGGTGGCCACTTCTCTTGGGTCACCGAGAGCGGGAAGCAAGAGCATCACCTCGTCGCAACGGTGGGAAAGTTCAGTGTGATATGGGACTTCCATCAGGTAAAGAACAGTGCACATGACTGCTACCGGAATCAGCAAGGACTCAAGAGCTGTTACTGCTACAAGATTGTGCTGAAAGATGAATCCATCGTCGAGAGCCGATTCATGCACGAAAAGTTTGCAGTCTCGGATTCTCCAGAAGCCCCACTGGTTGTGGCCACCCCCATGAAAGTCAGCTCCATCAGCCTATCTGGAAAGAGATGA

Protein sequence

MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDAKLKALKLKYGSSSPSQIPNANNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTSNVDGDSDDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRVDFVNKGVWALKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENADIEFGKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSHGIHGKGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKVVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIGGAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSPITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPAPRLLKLTPLDSHLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQGLKSCYCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR
Homology
BLAST of Csor.00g244250 vs. ExPASy Swiss-Prot
Match: P40781 (Protein CYPRO4 OS=Cynara cardunculus OX=4265 GN=CYPRO4 PE=2 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 4.1e-225
Identity = 382/494 (77.33%), Postives = 429/494 (86.84%), Query Frame = 0

Query: 164 MFGDQRRVDFVNKGVWALKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFI 223
           MFGDQRRVDFV+ GVWALKF  DE YR+FVT+FQ  LFENVYGL+A++ENKVK+YGK+FI
Sbjct: 1   MFGDQRRVDFVDSGVWALKFMRDEDYREFVTRFQSCLFENVYGLKASDENKVKVYGKDFI 60

Query: 224 GWLKPEVADDSIWENADIEFGKSPS--SSVRTKQDLIEEFEEAA-NGGVQSLTLGALDNS 283
           GW+KP+VADDS+WE  D E  +SPS  + VR   DL+EEFEEAA +GG+QS+ LGALDNS
Sbjct: 61  GWVKPDVADDSMWEKDDDELWRSPSKMTPVRGSNDLLEEFEEAASDGGIQSVALGALDNS 120

Query: 284 FLVNDSGVQVYRNLSHGIHGKGISLKF-------GAGNSPNSSRSTPKKGLLMKAETNML 343
           FLVNDSGVQV +N SHGIHGKG+ +KF       G  +S   S  TP+K LLM+ ETNML
Sbjct: 121 FLVNDSGVQVVKNFSHGIHGKGVYVKFDNGGKRVGGSSSGGYSNLTPQKALLMRGETNML 180

Query: 344 LMSPLKEGKPHASGLQQLDIETGKVVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFL 403
           LMSP K GKPH++G+ QLDIETGK+VTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFL
Sbjct: 181 LMSPAKAGKPHSTGVNQLDIETGKIVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFL 240

Query: 404 GLDDNRLCQWDMRDRRGMVQNIGGAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVG 463
           GLDDNRL QWDMRDRRGMVQNI  A  +SPVL+W QGHQFSRGTNFQ FATTGDGSIVVG
Sbjct: 241 GLDDNRLSQWDMRDRRGMVQNI--AHSDSPVLHWTQGHQFSRGTNFQSFATTGDGSIVVG 300

Query: 464 SVDGKIRLYSKTSMRQAKTAFPGLGSPITHVDVTHDGKWILGTTDTYLILICVLFVDKDG 523
           S+DGKIRLYS TSMR AKTAFPGLGSPITHVDVT+DGKWILGTTDTYLILIC LF DKDG
Sbjct: 301 SLDGKIRLYSTTSMRMAKTAFPGLGSPITHVDVTYDGKWILGTTDTYLILICSLFTDKDG 360

Query: 524 NTKTGFSGRMGNKIPAPRLLKLTPLDSHLAGTDNRFHGGHFSWVTESGKQEHHLVATVGK 583
            TKTGFSGRMGNKIPAPRLLKLTP+DSH AG +N+FHGG FSWVTESGKQE HLVATVGK
Sbjct: 361 KTKTGFSGRMGNKIPAPRLLKLTPVDSHTAGVNNKFHGGRFSWVTESGKQERHLVATVGK 420

Query: 584 FSVIWDFHQVKNSAHDCYRNQQGLKSCYCYKIVLKDESIVESRFMHEKF-AVSDSPEAPL 643
           FSVIWDF +VKNS H+CYRNQ+GLKSCYCYK++ KDESI+ES FM++K+ AV DSPEAPL
Sbjct: 421 FSVIWDFQRVKNSGHECYRNQEGLKSCYCYKLMTKDESIIESLFMNDKYAAVGDSPEAPL 480

Query: 644 VVATPMKVSSISLS 647
           V+ATP K++S S+S
Sbjct: 481 VMATPKKITSFSMS 492

BLAST of Csor.00g244250 vs. ExPASy Swiss-Prot
Match: Q1MTR3 (Vacuolar import and degradation protein 27 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=vid27 PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 1.2e-27
Identity = 126/468 (26.92%), Postives = 215/468 (45.94%), Query Frame = 0

Query: 144 WILKVGGKVRALVSTEMQLKMFGDQRRVDFVNKGVWALKFPTDEQYRDFVTKFQDYLFEN 203
           W+L+   +VR     E+ ++   +      +N+  W LK   DEQ        +DY+ E 
Sbjct: 326 WLLRFDSQVRMEQFQELLMRALWES-----LNQQRW-LKI-DDEQ--------RDYVMET 385

Query: 204 VYGLEATEENKVKIYGKEFIGWLKPEVADD----SIWENADIEFGKSPSSSVRTKQDLIE 263
            +  E  E+++ + + ++ +   K E  ++    S +E++  +F    +  +    D   
Sbjct: 386 FHEDEELEDSEDEEFARQQLLSRKEEEEEEDEEASDFEDSFADFSDGEADDL----DESR 445

Query: 264 EFEEAANGGVQSLTLG-ALDNSFLVNDSGVQVYRNLSHGIHGKGISLKFGAGN--SPNSS 323
             +EAA      L +G   D S++V ++ + V++++      KG+  +    N  +P   
Sbjct: 446 WRKEAAKEHNSLLAVGYKNDRSYVVRNNKIGVFKHVDE----KGLKFQTALNNLSTPKGK 505

Query: 324 RSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKVVTEWKFEKDGTDITMRDI 383
              P K +L   ++++L  +   E  PH+  L  +DIE GK+V EWK      D+ +   
Sbjct: 506 SLRPSKLMLHNQDSSILFQT---ENAPHS--LYHMDIEYGKIVDEWKVH---DDVPLVTF 565

Query: 384 TNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIGGAADNSPVLNWAQGHQFSRG 443
           T D K +Q+  +E T +GL +N + + D R              N  V    Q  Q++  
Sbjct: 566 TPDNKFAQM-TAEQTLIGLSNNSIFRIDPR-----------VEGNKLVAE--QFKQYATK 625

Query: 444 TNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSPITHVDVTHDGKWILGT 503
            +F   ATT +G I V S  G IRL+ +  +  AKTA P LG  I  VDVT  G ++L T
Sbjct: 626 NDFSSAATTENGYIAVASNKGDIRLFDRIGV-NAKTALPALGEAIIGVDVTASGDFVLAT 685

Query: 504 TDTYLILICVLFVDKDGNTKTG-FSGRMG-------NKIPAPRLLKLTPLDSHLAGTDNR 563
             TY++LI       D   K G ++GR+G       +K P P+ L+L+P   H+A     
Sbjct: 686 CKTYILLI-------DTRIKEGRYAGRLGFERNFAKDKKPKPKRLQLSP--QHIAMMQRE 738

Query: 564 FHGG----HFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYR 593
             GG       + T    +E  +V+++G F + W+  +VK    D Y+
Sbjct: 746 LKGGASFTPAKFNTGIDAKETTIVSSIGPFLISWNLDRVKRGFTDSYK 738

BLAST of Csor.00g244250 vs. ExPASy Swiss-Prot
Match: Q555V7 (VID27-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0274813 PE=3 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 4.7e-27
Identity = 126/509 (24.75%), Postives = 214/509 (42.04%), Query Frame = 0

Query: 116 VDGDSDDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRVDFVN 175
           +D +SDDD+EDEED      SG   +     + G +     S E Q+K          V 
Sbjct: 442 LDQESDDDEEDEED---EEESGEEEE-----ESGEEEEEESSEEEQVK--------QPVK 501

Query: 176 KGV-WALKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKE------FIGWLKP 235
           K +    K  ++E+  +   +  +   E   G E+ EE +     K+       +   KP
Sbjct: 502 KPIKQEPKEESEEEESEEEEEESEEEEEESEGEESEEEEEESEEEKDNGRKVFRVPLRKP 561

Query: 236 -----EVADDSIWENADIEFGKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLV 295
                E  +D   E +D +  +   S     +D  +  ++ +     SL +G  D S++V
Sbjct: 562 TTESEESDEDEDEEESDEDEDEESDSEENLSEDKKQISKDFSKSKNSSLIVGYKDKSYVV 621

Query: 296 NDSGVQVYRNLSHGIHGKGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKP 355
             S + V+     GI            +  N S   PKK +L + +  +L+++P K+   
Sbjct: 622 RGSTIGVFNTDDDGIKFNTAITNIKQKDGKNFS---PKKMMLQQQDQTLLMLNPEKK--- 681

Query: 356 HASGLQQLDIETGKVVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQW 415
             S + ++D+    +V EW             + +  K    + +   F+G + N +   
Sbjct: 682 --SNVYKMDLNRPDIVEEWDMNWRNQPTLTNSVVSQYKNDD-ETNNQLFVGYNGNNMFLV 741

Query: 416 DMRDRRGMVQ-NIGGAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLY 475
           D R+ +  V     G ++   V                C ATTG G I  G+  G+I+L+
Sbjct: 742 DPRESKNKVTVKFHGGSNPKSVTT--------------CAATTGSGQIAFGTSKGEIKLF 801

Query: 476 SKTSMRQ----------------AKTAFPGLGSPITHVDVTHDGKWILGTTDTYLILICV 535
           SKT   Q                ++T  PG+G PI  +DVT DGKWI+ T   Y+++I  
Sbjct: 802 SKTQFDQNKRSVTSSDPLGAIARSRTTLPGIGDPIVGIDVTKDGKWIVATCKQYIMVIPA 861

Query: 536 LFVDKDGNTKTGFSGRMGNKIPAPRLLKLTPLDSHLAGTDNRFHGGHFSWVTESGKQEHH 595
               KDG+  +GF  R+G + P+P+ L L P D    G    F    F+ V +  + E  
Sbjct: 862 SL--KDGS--SGFEDRLGARRPSPKRLILKPQDIKRMGGVVNFTPAKFNIVGDE-QSETS 906

BLAST of Csor.00g244250 vs. ExPASy Swiss-Prot
Match: P40157 (Vacuolar import and degradation protein 27 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=VID27 PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 7.5e-17
Identity = 107/403 (26.55%), Postives = 169/403 (41.94%), Query Frame = 0

Query: 229 EVADDSIWENADIEFGKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDN-SFLVNDSG 288
           E  +DS  E+ + +  K   SS     +  EE   A + G  SLT+   +N S++  D+ 
Sbjct: 385 ESENDSEDEDDENDHSKRIISS-----EAFEEPRRATSKGNSSLTVAFRNNRSYVTRDNR 444

Query: 289 VQVYRNLSHGIHGKGISLKFGAG----NSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKP 348
           + V++        +  SL+F A     ++       P K +L   + N++L     E K 
Sbjct: 445 IGVFKT-----DDEDDSLEFVAAIKNISNLGGKSIDPHKPMLYMEDRNLILTDGENENK- 504

Query: 349 HASGLQQLDIETGKVVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQW 408
               L ++DIE GKV+ EW       D  +       K  Q+ P E T +G+    + + 
Sbjct: 505 ----LYKMDIERGKVIEEW----STGDKNVVQYGPTKKFDQMTP-EQTIVGVSQKGVFKI 564

Query: 409 DMRDRRGMVQNIGGAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYS 468
           D R        I G   N   ++  +   +    NF    TT  G I +GS  G I+LY 
Sbjct: 565 DPR--------ING--KNKIAVD--ESKDYVGKYNFSSIGTTESGYIAIGSEKGDIKLYD 624

Query: 469 KTSMRQAKTAFPGLGSPITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRM 528
           +  +R AKTA P LG  I  +  + DGKW+L T ++ L+L+ +   D       GF    
Sbjct: 625 RLGIR-AKTAIPSLGQAIKFITTSADGKWLLATCESTLLLMDLKIKDGKNAGNIGFL--- 684

Query: 529 GNKIPAPR-----LLKLTPLDSHLAGTDN----RFHGGHFSWVTESGKQEHHLVATVGKF 588
               PA       +LK+ P  S    T      RF   +F+  T  G+QE  +V + G +
Sbjct: 685 -KSFPASENVKTYVLKIRPEHSASILTYTKKPIRFTKAYFN--TGIGQQEQTIVTSTGPY 739

Query: 589 SVIWDFHQVKNSAHDCYRNQQGLKSCYCYKIVLKDESIVESRF 618
           ++ W    +         NQ G  + Y Y+I   +  +V   F
Sbjct: 745 AISWSLKGI--------LNQDGSNN-YPYRIRRYNADVVADNF 739

BLAST of Csor.00g244250 vs. NCBI nr
Match: KAG6576955.1 (Protein CYPRO4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1290 bits (3339), Expect = 0.0
Identity = 649/649 (100.00%), Postives = 649/649 (100.00%), Query Frame = 0

Query: 1   MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA 60
           MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA
Sbjct: 1   MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA 60

Query: 61  KLKALKLKYGSSSPSQIPNANNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTSNVDGDS 120
           KLKALKLKYGSSSPSQIPNANNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTSNVDGDS
Sbjct: 61  KLKALKLKYGSSSPSQIPNANNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTSNVDGDS 120

Query: 121 DDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRVDFVNKGVWA 180
           DDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRVDFVNKGVWA
Sbjct: 121 DDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRVDFVNKGVWA 180

Query: 181 LKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENAD 240
           LKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENAD
Sbjct: 181 LKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENAD 240

Query: 241 IEFGKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSHGIHG 300
           IEFGKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSHGIHG
Sbjct: 241 IEFGKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSHGIHG 300

Query: 301 KGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKVVT 360
           KGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKVVT
Sbjct: 301 KGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKVVT 360

Query: 361 EWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIGGAAD 420
           EWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIGGAAD
Sbjct: 361 EWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIGGAAD 420

Query: 421 NSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSP 480
           NSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSP
Sbjct: 421 NSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSP 480

Query: 481 ITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPAPRLLKLTPLDS 540
           ITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPAPRLLKLTPLDS
Sbjct: 481 ITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPAPRLLKLTPLDS 540

Query: 541 HLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQGLKSC 600
           HLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQGLKSC
Sbjct: 541 HLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQGLKSC 600

Query: 601 YCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR 649
           YCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR
Sbjct: 601 YCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR 649

BLAST of Csor.00g244250 vs. NCBI nr
Match: KAG7014976.1 (Protein CYPRO4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1290 bits (3339), Expect = 0.0
Identity = 649/649 (100.00%), Postives = 649/649 (100.00%), Query Frame = 0

Query: 1   MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA 60
           MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA
Sbjct: 116 MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA 175

Query: 61  KLKALKLKYGSSSPSQIPNANNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTSNVDGDS 120
           KLKALKLKYGSSSPSQIPNANNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTSNVDGDS
Sbjct: 176 KLKALKLKYGSSSPSQIPNANNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTSNVDGDS 235

Query: 121 DDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRVDFVNKGVWA 180
           DDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRVDFVNKGVWA
Sbjct: 236 DDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRVDFVNKGVWA 295

Query: 181 LKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENAD 240
           LKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENAD
Sbjct: 296 LKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENAD 355

Query: 241 IEFGKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSHGIHG 300
           IEFGKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSHGIHG
Sbjct: 356 IEFGKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSHGIHG 415

Query: 301 KGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKVVT 360
           KGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKVVT
Sbjct: 416 KGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKVVT 475

Query: 361 EWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIGGAAD 420
           EWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIGGAAD
Sbjct: 476 EWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIGGAAD 535

Query: 421 NSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSP 480
           NSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSP
Sbjct: 536 NSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSP 595

Query: 481 ITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPAPRLLKLTPLDS 540
           ITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPAPRLLKLTPLDS
Sbjct: 596 ITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPAPRLLKLTPLDS 655

Query: 541 HLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQGLKSC 600
           HLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQGLKSC
Sbjct: 656 HLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQGLKSC 715

Query: 601 YCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR 649
           YCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR
Sbjct: 716 YCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR 764

BLAST of Csor.00g244250 vs. NCBI nr
Match: XP_022922790.1 (protein CYPRO4-like [Cucurbita moschata])

HSP 1 Score: 1286 bits (3329), Expect = 0.0
Identity = 647/649 (99.69%), Postives = 647/649 (99.69%), Query Frame = 0

Query: 1   MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA 60
           MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA
Sbjct: 1   MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA 60

Query: 61  KLKALKLKYGSSSPSQIPNANNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTSNVDGDS 120
           KLKALKLKYGSSSPSQIPNANNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTSNVDGDS
Sbjct: 61  KLKALKLKYGSSSPSQIPNANNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTSNVDGDS 120

Query: 121 DDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRVDFVNKGVWA 180
           DDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRVDFVNKGVWA
Sbjct: 121 DDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRVDFVNKGVWA 180

Query: 181 LKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENAD 240
           LKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENAD
Sbjct: 181 LKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENAD 240

Query: 241 IEFGKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSHGIHG 300
           IEFGKSP SSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSHGIHG
Sbjct: 241 IEFGKSPGSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSHGIHG 300

Query: 301 KGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKVVT 360
           KGISLKFGAGNSPNSSRSTPKKGLL KAETNMLLMSPLKEGKPHASGLQQLDIETGKVVT
Sbjct: 301 KGISLKFGAGNSPNSSRSTPKKGLLTKAETNMLLMSPLKEGKPHASGLQQLDIETGKVVT 360

Query: 361 EWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIGGAAD 420
           EWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIGGAAD
Sbjct: 361 EWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIGGAAD 420

Query: 421 NSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSP 480
           NSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSP
Sbjct: 421 NSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSP 480

Query: 481 ITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPAPRLLKLTPLDS 540
           ITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPAPRLLKLTPLDS
Sbjct: 481 ITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPAPRLLKLTPLDS 540

Query: 541 HLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQGLKSC 600
           HLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQGLKSC
Sbjct: 541 HLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQGLKSC 600

Query: 601 YCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR 649
           YCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR
Sbjct: 601 YCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR 649

BLAST of Csor.00g244250 vs. NCBI nr
Match: XP_023552532.1 (protein CYPRO4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1285 bits (3325), Expect = 0.0
Identity = 645/649 (99.38%), Postives = 648/649 (99.85%), Query Frame = 0

Query: 1   MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA 60
           MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA
Sbjct: 1   MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA 60

Query: 61  KLKALKLKYGSSSPSQIPNANNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTSNVDGDS 120
           KLKALKLKYGSSSPSQIPNA NAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKT+NVDGDS
Sbjct: 61  KLKALKLKYGSSSPSQIPNAKNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTANVDGDS 120

Query: 121 DDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRVDFVNKGVWA 180
           DDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRR+DFVNKGVWA
Sbjct: 121 DDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRIDFVNKGVWA 180

Query: 181 LKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENAD 240
           LKFP+DEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENAD
Sbjct: 181 LKFPSDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENAD 240

Query: 241 IEFGKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSHGIHG 300
           IEFGKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSHGIHG
Sbjct: 241 IEFGKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSHGIHG 300

Query: 301 KGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKVVT 360
           KGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKVVT
Sbjct: 301 KGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKVVT 360

Query: 361 EWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIGGAAD 420
           EWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIGGAAD
Sbjct: 361 EWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIGGAAD 420

Query: 421 NSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSP 480
           NSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSP
Sbjct: 421 NSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSP 480

Query: 481 ITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPAPRLLKLTPLDS 540
           ITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPAPRLLKLTPLDS
Sbjct: 481 ITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPAPRLLKLTPLDS 540

Query: 541 HLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQGLKSC 600
           HLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQGLKSC
Sbjct: 541 HLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQGLKSC 600

Query: 601 YCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR 649
           YCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR
Sbjct: 601 YCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR 649

BLAST of Csor.00g244250 vs. NCBI nr
Match: XP_022984689.1 (protein CYPRO4-like [Cucurbita maxima])

HSP 1 Score: 1277 bits (3304), Expect = 0.0
Identity = 641/649 (98.77%), Postives = 646/649 (99.54%), Query Frame = 0

Query: 1   MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA 60
           MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA
Sbjct: 1   MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA 60

Query: 61  KLKALKLKYGSSSPSQIPNANNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTSNVDGDS 120
           KLKALKLKYGSSSPSQIPNA+NAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKT+NVDGDS
Sbjct: 61  KLKALKLKYGSSSPSQIPNASNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTANVDGDS 120

Query: 121 DDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRVDFVNKGVWA 180
           DDDQED+ D YKANSSGGRGQLRWILKVG KVRALVSTEMQLKMFGDQRRVDFVNKGVWA
Sbjct: 121 DDDQEDDYDAYKANSSGGRGQLRWILKVGAKVRALVSTEMQLKMFGDQRRVDFVNKGVWA 180

Query: 181 LKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENAD 240
           LKFP+DEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENAD
Sbjct: 181 LKFPSDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENAD 240

Query: 241 IEFGKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSHGIHG 300
           IEFGKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSHGIHG
Sbjct: 241 IEFGKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSHGIHG 300

Query: 301 KGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKVVT 360
           KGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKVVT
Sbjct: 301 KGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKVVT 360

Query: 361 EWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIGGAAD 420
           EWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIGGAAD
Sbjct: 361 EWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIGGAAD 420

Query: 421 NSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSP 480
           NSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSP
Sbjct: 421 NSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSP 480

Query: 481 ITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPAPRLLKLTPLDS 540
           ITHVDVTHDGKWILGTTDTYLILICVLF+DKDGNTKTGFSGRMGNKIPAPRLLKLTPLDS
Sbjct: 481 ITHVDVTHDGKWILGTTDTYLILICVLFIDKDGNTKTGFSGRMGNKIPAPRLLKLTPLDS 540

Query: 541 HLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQGLKSC 600
           HLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQGLKSC
Sbjct: 541 HLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQGLKSC 600

Query: 601 YCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR 649
           YCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR
Sbjct: 601 YCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR 649

BLAST of Csor.00g244250 vs. ExPASy TrEMBL
Match: A0A6J1E9T2 (protein CYPRO4-like OS=Cucurbita moschata OX=3662 GN=LOC111430675 PE=4 SV=1)

HSP 1 Score: 1286 bits (3329), Expect = 0.0
Identity = 647/649 (99.69%), Postives = 647/649 (99.69%), Query Frame = 0

Query: 1   MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA 60
           MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA
Sbjct: 1   MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA 60

Query: 61  KLKALKLKYGSSSPSQIPNANNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTSNVDGDS 120
           KLKALKLKYGSSSPSQIPNANNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTSNVDGDS
Sbjct: 61  KLKALKLKYGSSSPSQIPNANNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTSNVDGDS 120

Query: 121 DDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRVDFVNKGVWA 180
           DDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRVDFVNKGVWA
Sbjct: 121 DDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRVDFVNKGVWA 180

Query: 181 LKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENAD 240
           LKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENAD
Sbjct: 181 LKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENAD 240

Query: 241 IEFGKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSHGIHG 300
           IEFGKSP SSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSHGIHG
Sbjct: 241 IEFGKSPGSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSHGIHG 300

Query: 301 KGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKVVT 360
           KGISLKFGAGNSPNSSRSTPKKGLL KAETNMLLMSPLKEGKPHASGLQQLDIETGKVVT
Sbjct: 301 KGISLKFGAGNSPNSSRSTPKKGLLTKAETNMLLMSPLKEGKPHASGLQQLDIETGKVVT 360

Query: 361 EWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIGGAAD 420
           EWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIGGAAD
Sbjct: 361 EWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIGGAAD 420

Query: 421 NSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSP 480
           NSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSP
Sbjct: 421 NSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSP 480

Query: 481 ITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPAPRLLKLTPLDS 540
           ITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPAPRLLKLTPLDS
Sbjct: 481 ITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPAPRLLKLTPLDS 540

Query: 541 HLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQGLKSC 600
           HLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQGLKSC
Sbjct: 541 HLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQGLKSC 600

Query: 601 YCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR 649
           YCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR
Sbjct: 601 YCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR 649

BLAST of Csor.00g244250 vs. ExPASy TrEMBL
Match: A0A6J1JB96 (protein CYPRO4-like OS=Cucurbita maxima OX=3661 GN=LOC111482893 PE=4 SV=1)

HSP 1 Score: 1277 bits (3304), Expect = 0.0
Identity = 641/649 (98.77%), Postives = 646/649 (99.54%), Query Frame = 0

Query: 1   MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA 60
           MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA
Sbjct: 1   MGTAQSREDAELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTGTASAVDDVDA 60

Query: 61  KLKALKLKYGSSSPSQIPNANNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTSNVDGDS 120
           KLKALKLKYGSSSPSQIPNA+NAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKT+NVDGDS
Sbjct: 61  KLKALKLKYGSSSPSQIPNASNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTANVDGDS 120

Query: 121 DDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRVDFVNKGVWA 180
           DDDQED+ D YKANSSGGRGQLRWILKVG KVRALVSTEMQLKMFGDQRRVDFVNKGVWA
Sbjct: 121 DDDQEDDYDAYKANSSGGRGQLRWILKVGAKVRALVSTEMQLKMFGDQRRVDFVNKGVWA 180

Query: 181 LKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENAD 240
           LKFP+DEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENAD
Sbjct: 181 LKFPSDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDSIWENAD 240

Query: 241 IEFGKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSHGIHG 300
           IEFGKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSHGIHG
Sbjct: 241 IEFGKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNLSHGIHG 300

Query: 301 KGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKVVT 360
           KGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKVVT
Sbjct: 301 KGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDIETGKVVT 360

Query: 361 EWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIGGAAD 420
           EWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIGGAAD
Sbjct: 361 EWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQNIGGAAD 420

Query: 421 NSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSP 480
           NSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSP
Sbjct: 421 NSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTAFPGLGSP 480

Query: 481 ITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPAPRLLKLTPLDS 540
           ITHVDVTHDGKWILGTTDTYLILICVLF+DKDGNTKTGFSGRMGNKIPAPRLLKLTPLDS
Sbjct: 481 ITHVDVTHDGKWILGTTDTYLILICVLFIDKDGNTKTGFSGRMGNKIPAPRLLKLTPLDS 540

Query: 541 HLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQGLKSC 600
           HLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQGLKSC
Sbjct: 541 HLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRNQQGLKSC 600

Query: 601 YCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR 649
           YCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR
Sbjct: 601 YCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR 649

BLAST of Csor.00g244250 vs. ExPASy TrEMBL
Match: A0A5D3DQK8 (Protein CYPRO4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1441G00020 PE=4 SV=1)

HSP 1 Score: 1165 bits (3013), Expect = 0.0
Identity = 588/660 (89.09%), Postives = 625/660 (94.70%), Query Frame = 0

Query: 1   MGTAQSREDAELSDSDEYKEEEEEDDE---EEEYEDADEVLKPQSISS-AKSTGTASAVD 60
           MG+ QSRED ELSDSD+YKEEEE+DD+   EEEY+DAD+ LKP S+S+ AKSTGT+SA+D
Sbjct: 1   MGSGQSREDVELSDSDDYKEEEEDDDDDDDEEEYQDADKELKPHSVSTTAKSTGTSSAID 60

Query: 61  DVDAKLKALKLKYGSSS------PSQIPNANNAVKLYLHIGGNTPRAKWIVSEKLTFYAF 120
           DVDAKLKALKLKYGSSS      PS+ PN+ NAVKLYLHIGGNTPRAKWIVSEKL+FYAF
Sbjct: 61  DVDAKLKALKLKYGSSSSSPSPSPSKTPNSKNAVKLYLHIGGNTPRAKWIVSEKLSFYAF 120

Query: 121 LKTSNVDGDSDDDQEDEED-GYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQR 180
           LKT+NVDG +DDD+E+++D GY ANSSGGR   RW+LKVG KVRALVSTEMQLKMFGDQR
Sbjct: 121 LKTANVDGHNDDDEEEDDDQGYNANSSGGR---RWVLKVGAKVRALVSTEMQLKMFGDQR 180

Query: 181 RVDFVNKGVWALKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPE 240
           RVDFVNKGVWALKFP+ EQYR+FVT+FQD LFENVYGL+AT+ENKVKIYGKEFIGWLKPE
Sbjct: 181 RVDFVNKGVWALKFPSGEQYRNFVTEFQDCLFENVYGLQATDENKVKIYGKEFIGWLKPE 240

Query: 241 VADDSIWENADIEFGKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQ 300
           VADDSIWENADIEF KSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQ
Sbjct: 241 VADDSIWENADIEFEKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQ 300

Query: 301 VYRNLSHGIHGKGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQ 360
           VYRNLSHGIHGKG+SLKFGAG+SPN  RSTPKKGLLMKAETNMLLMSPLKEGKPH +GL+
Sbjct: 301 VYRNLSHGIHGKGVSLKFGAGHSPNVWRSTPKKGLLMKAETNMLLMSPLKEGKPHTTGLE 360

Query: 361 QLDIETGKVVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRR 420
           QLDIETGK+VTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRR
Sbjct: 361 QLDIETGKIVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRR 420

Query: 421 GMVQNIGGAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQ 480
           GMVQNI G+ADNS VLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQ
Sbjct: 421 GMVQNISGSADNSTVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQ 480

Query: 481 AKTAFPGLGSPITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPA 540
           AKTAFPGLGSPITHVDVT+DGKWILGTTD+YLILIC LF DKDGNTKTGFSGRMGN+IPA
Sbjct: 481 AKTAFPGLGSPITHVDVTYDGKWILGTTDSYLILICTLFTDKDGNTKTGFSGRMGNRIPA 540

Query: 541 PRLLKLTPLDSHLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHD 600
           PRLLKLTPLDSHLAGTDN FHGGHFSWVTESGKQE HLVATVGKFSVIWDFH+VKNS+HD
Sbjct: 541 PRLLKLTPLDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHRVKNSSHD 600

Query: 601 CYRNQQGLKSCYCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR 649
           CYRNQQGLKSCYCYKIVLKDESIVESRFMH+KFAVSDSPEAPLVVATPMKVSSISLSGKR
Sbjct: 601 CYRNQQGLKSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLSGKR 657

BLAST of Csor.00g244250 vs. ExPASy TrEMBL
Match: A0A1S3C231 (protein CYPRO4 OS=Cucumis melo OX=3656 GN=LOC103496156 PE=4 SV=1)

HSP 1 Score: 1165 bits (3013), Expect = 0.0
Identity = 588/660 (89.09%), Postives = 625/660 (94.70%), Query Frame = 0

Query: 1   MGTAQSREDAELSDSDEYKEEEEEDDE---EEEYEDADEVLKPQSISS-AKSTGTASAVD 60
           MG+ QSRED ELSDSD+YKEEEE+DD+   EEEY+DAD+ LKP S+S+ AKSTGT+SA+D
Sbjct: 1   MGSGQSREDVELSDSDDYKEEEEDDDDDDDEEEYQDADKELKPHSVSTTAKSTGTSSAID 60

Query: 61  DVDAKLKALKLKYGSSS------PSQIPNANNAVKLYLHIGGNTPRAKWIVSEKLTFYAF 120
           DVDAKLKALKLKYGSSS      PS+ PN+ NAVKLYLHIGGNTPRAKWIVSEKL+FYAF
Sbjct: 61  DVDAKLKALKLKYGSSSSSPSPSPSKTPNSKNAVKLYLHIGGNTPRAKWIVSEKLSFYAF 120

Query: 121 LKTSNVDGDSDDDQEDEED-GYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQR 180
           LKT+NVDG +DDD+E+++D GY ANSSGGR   RW+LKVG KVRALVSTEMQLKMFGDQR
Sbjct: 121 LKTANVDGHNDDDEEEDDDQGYNANSSGGR---RWVLKVGAKVRALVSTEMQLKMFGDQR 180

Query: 181 RVDFVNKGVWALKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPE 240
           RVDFVNKGVWALKFP+ EQYR+FVT+FQD LFENVYGL+AT+ENKVKIYGKEFIGWLKPE
Sbjct: 181 RVDFVNKGVWALKFPSGEQYRNFVTEFQDCLFENVYGLQATDENKVKIYGKEFIGWLKPE 240

Query: 241 VADDSIWENADIEFGKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQ 300
           VADDSIWENADIEF KSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQ
Sbjct: 241 VADDSIWENADIEFEKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQ 300

Query: 301 VYRNLSHGIHGKGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQ 360
           VYRNLSHGIHGKG+SLKFGAG+SPN  RSTPKKGLLMKAETNMLLMSPLKEGKPH +GL+
Sbjct: 301 VYRNLSHGIHGKGVSLKFGAGHSPNVWRSTPKKGLLMKAETNMLLMSPLKEGKPHTTGLE 360

Query: 361 QLDIETGKVVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRR 420
           QLDIETGK+VTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRR
Sbjct: 361 QLDIETGKIVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRR 420

Query: 421 GMVQNIGGAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQ 480
           GMVQNI G+ADNS VLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQ
Sbjct: 421 GMVQNISGSADNSTVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQ 480

Query: 481 AKTAFPGLGSPITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPA 540
           AKTAFPGLGSPITHVDVT+DGKWILGTTD+YLILIC LF DKDGNTKTGFSGRMGN+IPA
Sbjct: 481 AKTAFPGLGSPITHVDVTYDGKWILGTTDSYLILICTLFTDKDGNTKTGFSGRMGNRIPA 540

Query: 541 PRLLKLTPLDSHLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHD 600
           PRLLKLTPLDSHLAGTDN FHGGHFSWVTESGKQE HLVATVGKFSVIWDFH+VKNS+HD
Sbjct: 541 PRLLKLTPLDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHRVKNSSHD 600

Query: 601 CYRNQQGLKSCYCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR 649
           CYRNQQGLKSCYCYKIVLKDESIVESRFMH+KFAVSDSPEAPLVVATPMKVSSISLSGKR
Sbjct: 601 CYRNQQGLKSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLSGKR 657

BLAST of Csor.00g244250 vs. ExPASy TrEMBL
Match: A0A0A0LAA1 (VID27 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G207380 PE=4 SV=1)

HSP 1 Score: 1162 bits (3006), Expect = 0.0
Identity = 585/656 (89.18%), Postives = 623/656 (94.97%), Query Frame = 0

Query: 1   MGTAQSREDAELSDSDEYKEEEEEDDE-EEEYEDADEVLKPQSISS-AKSTGTASAVDDV 60
           MG+ QSRED ELSDSD+YKEEEE+DD+ EEEYEDAD+ LKP SIS+ AKSTGT+SA+DDV
Sbjct: 1   MGSGQSREDVELSDSDDYKEEEEDDDDDEEEYEDADKELKPHSISTTAKSTGTSSAIDDV 60

Query: 61  DAKLKALKLKYGSSS----PSQIPNANNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTS 120
           DAKLKALKLKYGSSS    PSQ PN+ NAVKLYLHIGGNTPRAKWIVSEK TFY FLKT+
Sbjct: 61  DAKLKALKLKYGSSSSSPSPSQTPNSKNAVKLYLHIGGNTPRAKWIVSEKFTFYVFLKTA 120

Query: 121 NVDGDSDDDQEDEED-GYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRVDF 180
           NVDG +DDD+E+++D G+ ANSSGGR   RW+LKVG KVRALVSTEMQLKMFG+QRRVDF
Sbjct: 121 NVDGHNDDDEEEDDDEGHNANSSGGR---RWVLKVGAKVRALVSTEMQLKMFGEQRRVDF 180

Query: 181 VNKGVWALKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADD 240
           VNKGVWALKFP+ EQYR+FVT+FQD LFENVYGL+AT+ENKVKIYGKEFIGWLKPEVADD
Sbjct: 181 VNKGVWALKFPSGEQYRNFVTEFQDCLFENVYGLQATDENKVKIYGKEFIGWLKPEVADD 240

Query: 241 SIWENADIEFGKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRN 300
           SIWENADI+F KSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRN
Sbjct: 241 SIWENADIDFEKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRN 300

Query: 301 LSHGIHGKGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQLDI 360
           LSHGIHGKG+SLKFGAG+SPN  RSTPKKGLL+KAETNMLLMSPLKEGKPH +GL+QLDI
Sbjct: 301 LSHGIHGKGVSLKFGAGHSPNIGRSTPKKGLLVKAETNMLLMSPLKEGKPHTTGLEQLDI 360

Query: 361 ETGKVVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQ 420
           ETGK+VTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQ
Sbjct: 361 ETGKIVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGMVQ 420

Query: 421 NIGGAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAKTA 480
           NI G+ADNS VLNWAQGHQFSRGTNFQCFATTGDGSIVVGS+DGKIRLYSKTSMRQAKTA
Sbjct: 421 NISGSADNSMVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSIDGKIRLYSKTSMRQAKTA 480

Query: 481 FPGLGSPITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPAPRLL 540
           FPGLGSPITHVDVT+DGKWILGTTD+YLILIC LF DKDGNTKTGFSGRMGN+IPAPRLL
Sbjct: 481 FPGLGSPITHVDVTYDGKWILGTTDSYLILICTLFTDKDGNTKTGFSGRMGNRIPAPRLL 540

Query: 541 KLTPLDSHLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCYRN 600
           KLTPLDSHLAGTDN FHGGHFSWVTESGKQE HLVATVGKFSVIWDFH+VKNS+HDCYRN
Sbjct: 541 KLTPLDSHLAGTDNTFHGGHFSWVTESGKQERHLVATVGKFSVIWDFHRVKNSSHDCYRN 600

Query: 601 QQGLKSCYCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGKR 649
           QQGLKSCYCYKIVLKDESIVESRFMH+KFAVSDSPEAPLVVATPMKVSSISLSGKR
Sbjct: 601 QQGLKSCYCYKIVLKDESIVESRFMHDKFAVSDSPEAPLVVATPMKVSSISLSGKR 653

BLAST of Csor.00g244250 vs. TAIR 10
Match: AT3G19240.1 (Vacuolar import/degradation, Vid27-related protein )

HSP 1 Score: 926.0 bits (2392), Expect = 1.7e-269
Identity = 476/660 (72.12%), Postives = 548/660 (83.03%), Query Frame = 0

Query: 1   MGTAQSREDA-----ELSDSDEYKEEEEEDDEEEEYEDADEVLKPQSISSAKSTG-TASA 60
           MGT+QSRED        SDSD Y+EE+E+  ++ + + +  V   +   S+ S+G T S+
Sbjct: 1   MGTSQSREDRISESDTESDSDYYEEEDEDQYDDAKKQGSSSVSGTRPSDSSSSSGPTDSS 60

Query: 61  VDDVDAKLKALKLKYGSSSPSQIPNANNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLKTS 120
             D++ KLKALKLKY SSS S  P   NAVKLY HIGGNTP+AKWIVS+K+T Y F+KTS
Sbjct: 61  SLDIEQKLKALKLKYPSSS-SVTPKMKNAVKLYRHIGGNTPKAKWIVSDKMTSYKFVKTS 120

Query: 121 NVDGDSDDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRVDFV 180
           +VDG+  DD +D E+      SG  G+  W L VG KV+A VST+MQLKMFGDQRRVDFV
Sbjct: 121 SVDGEDIDDYDDCEE------SGEGGESFWFLGVGTKVKARVSTDMQLKMFGDQRRVDFV 180

Query: 181 NKGVWALKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVADDS 240
           + GVWALKF TDE YR FVT+FQDYLFENVY + A+EEN+VK+YGK+FIGW  PE ADDS
Sbjct: 181 SNGVWALKFLTDEDYRKFVTRFQDYLFENVYKIRASEENRVKVYGKDFIGWANPEAADDS 240

Query: 241 IWENADIEFGKSPSSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGVQVYRNL 300
           +WE+A+    +  +   R   DL EEFEE ANGGVQSLTLGALDNSFLVND GVQVYRN+
Sbjct: 241 MWEDAEAPPEEEETQGKR-NTDLTEEFEEVANGGVQSLTLGALDNSFLVNDYGVQVYRNM 300

Query: 301 SHGIHGKGISLKFGAGNS---PNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGLQQL 360
             GIHGKG+ ++F +GNS     SS++TP K LLM+AETNM+LMSP K+GKP+++G++QL
Sbjct: 301 ERGIHGKGVCVRFDSGNSKFGSGSSQTTPNKALLMRAETNMMLMSPAKQGKPNSTGVKQL 360

Query: 361 DIETGKVVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGM 420
           DIE+GK+VTEWKFEKDGT+ITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRG+
Sbjct: 361 DIESGKIVTEWKFEKDGTEITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDRRGI 420

Query: 421 VQNIGGAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMRQAK 480
           VQNI     +SP+L W QGHQFSRGTNFQCFATTGDGSIVVGS+DGKIRLYSKTSMR AK
Sbjct: 421 VQNI-----DSPILEWTQGHQFSRGTNFQCFATTGDGSIVVGSLDGKIRLYSKTSMRMAK 480

Query: 481 TAFPGLGSPITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIPAPR 540
           TAFPGLGSPITHVDV++DGKWILGTTDTYL+LIC LF DK+G TKTGFSGRMGNKIPAPR
Sbjct: 481 TAFPGLGSPITHVDVSYDGKWILGTTDTYLVLICTLFTDKNGLTKTGFSGRMGNKIPAPR 540

Query: 541 LLKLTPLDSHLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAHDCY 600
           LLKLTPLDSHLAG DN+FHGGHFSWVTESGKQE H+VATVGKFSVIWD  +VKNSAH+CY
Sbjct: 541 LLKLTPLDSHLAGKDNKFHGGHFSWVTESGKQERHIVATVGKFSVIWDLERVKNSAHECY 600

Query: 601 RNQQGLKSCYCYKIVLKDESIVESRFMHEKFAVS--DSPEAPLVVATPMKVSSISLSGKR 650
           RNQQGLKSCYCYKI+LKDESIVESRFMH+ F+ S   SPEAPLVVATP+KVSSISLSGKR
Sbjct: 601 RNQQGLKSCYCYKILLKDESIVESRFMHDNFSFSGNKSPEAPLVVATPLKVSSISLSGKR 647

BLAST of Csor.00g244250 vs. TAIR 10
Match: AT4G33400.1 (Vacuolar import/degradation, Vid27-related protein )

HSP 1 Score: 800.0 bits (2065), Expect = 1.4e-231
Identity = 412/661 (62.33%), Postives = 505/661 (76.40%), Query Frame = 0

Query: 1   MGTAQSREDAEL--SDSDEYKEEE---EEDDEEEEYEDA-DEVLKPQSISS--AKSTGTA 60
           MG + S ED E+  SD DEY+E E   E ++EEE ++D+ D+ L   S      +    +
Sbjct: 1   MGASHSHEDLEICSSDEDEYEEYEEGREGEEEEETFQDSRDDTLGISSSGGRRLRPKSPS 60

Query: 61  SAVDDVDAKLKALKLKYGSSSPSQIPNANNAVKLYLHIGGNTPRAKWIVSEKLTFYAFLK 120
           S++DDV+AKL+ALKLKY  +  +  P+  N+ +L+ +I GNTP+AKW+ +EKLT Y F+K
Sbjct: 61  SSLDDVEAKLQALKLKYPLTQSA--PSTQNSARLFRYINGNTPKAKWVTAEKLTAYCFVK 120

Query: 121 TSNVDGDSDDDQEDEEDGYKANSSGGRGQLRWILKVGGKVRALVSTEMQLKMFGDQRRVD 180
           T+  D   +DD++D+E+G   N         WILKVG K+R  VS EMQLK + DQRRVD
Sbjct: 121 TNKGD---EDDEDDDENGDVENE-------WWILKVGSKIREKVSDEMQLKAYKDQRRVD 180

Query: 181 FVNKGVWALKFPTDEQYRDFVTKFQDYLFENVYGLEATEENKVKIYGKEFIGWLKPEVAD 240
           FV K VWA+KF + E +  FV+ + + LFEN +G+E  E NK KIYGK+FIGW  PE AD
Sbjct: 181 FVAKAVWAMKFASSEDFSVFVSSYNNCLFENNHGVEFNEANKAKIYGKDFIGWANPEAAD 240

Query: 241 DSIWENADIEFGKSP----SSSVRTKQDLIEEFEEAANGGVQSLTLGALDNSFLVNDSGV 300
           DS+WE+AD    +SP    ++  R  QDL E FEEA + G+ SL LGALDNSFLV DSG+
Sbjct: 241 DSMWEDADDILLQSPGGGSATPARDTQDLTEAFEEATSEGIHSLALGALDNSFLVGDSGI 300

Query: 301 QVYRNLSHGIHGKGISLKFGAGNSPNSSRSTPKKGLLMKAETNMLLMSPLKEGKPHASGL 360
           QV++N+  GI GKG+ + F  G     S S PKK LLM+AETNMLLMSP+ +  PH+ G+
Sbjct: 301 QVFKNMRQGIQGKGVCVNFEPGYGRTHS-SAPKKALLMRAETNMLLMSPMSQ-TPHSRGI 360

Query: 361 QQLDIETGKVVTEWKFEKDGTDITMRDITNDTKGSQLDPSESTFLGLDDNRLCQWDMRDR 420
            QLDIETGK+++EWKFEKDG DI+M DITND KG+QLDPS STFLGLD+NRLC+WDMRDR
Sbjct: 361 HQLDIETGKIISEWKFEKDGVDISMSDITNDGKGAQLDPSASTFLGLDNNRLCRWDMRDR 420

Query: 421 RGMVQNIGGAADNSPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSVDGKIRLYSKTSMR 480
            GMVQ++  A  N+PVLNWAQGHQFSRGTNFQCFATTGDGSIVVGS+DGKIRLYS  +MR
Sbjct: 421 YGMVQDL--ATANTPVLNWAQGHQFSRGTNFQCFATTGDGSIVVGSLDGKIRLYSSNTMR 480

Query: 481 QAKTAFPGLGSPITHVDVTHDGKWILGTTDTYLILICVLFVDKDGNTKTGFSGRMGNKIP 540
           QAKTAFPGLG+P+THVD T DGKWI+GTTDTYLI+IC LF DK G TKTGF GRMGNKI 
Sbjct: 481 QAKTAFPGLGAPVTHVDATFDGKWIVGTTDTYLIVICTLFTDKSGKTKTGFEGRMGNKIA 540

Query: 541 APRLLKLTPLDSHLAGTDNRFHGGHFSWVTESGKQEHHLVATVGKFSVIWDFHQVKNSAH 600
           APRLLKL PLD+HLAG+DN+F    FSWVTE GKQE H+VATVGKFSVIW+F QVKN +H
Sbjct: 541 APRLLKLRPLDAHLAGSDNKFRNAQFSWVTEDGKQERHVVATVGKFSVIWNFQQVKNGSH 600

Query: 601 DCYRNQQGLKSCYCYKIVLKDESIVESRFMHEKFAVSDSPEAPLVVATPMKVSSISLSGK 650
           +CY +Q+GLK CYCYKIVL++ESIV+SRFM++ FA+S SPEAPLV+ATPMKVSS SLS K
Sbjct: 601 ECYHDQEGLKKCYCYKIVLRNESIVDSRFMNDNFAISGSPEAPLVIATPMKVSSFSLSSK 645

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P407814.1e-22577.33Protein CYPRO4 OS=Cynara cardunculus OX=4265 GN=CYPRO4 PE=2 SV=1[more]
Q1MTR31.2e-2726.92Vacuolar import and degradation protein 27 OS=Schizosaccharomyces pombe (strain ... [more]
Q555V74.7e-2724.75VID27-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0274813 PE=3 SV=... [more]
P401577.5e-1726.55Vacuolar import and degradation protein 27 OS=Saccharomyces cerevisiae (strain A... [more]
Match NameE-valueIdentityDescription
KAG6576955.10.0100.00Protein CYPRO4, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7014976.10.0100.00Protein CYPRO4, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022922790.10.099.69protein CYPRO4-like [Cucurbita moschata][more]
XP_023552532.10.099.38protein CYPRO4-like [Cucurbita pepo subsp. pepo][more]
XP_022984689.10.098.77protein CYPRO4-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1E9T20.099.69protein CYPRO4-like OS=Cucurbita moschata OX=3662 GN=LOC111430675 PE=4 SV=1[more]
A0A6J1JB960.098.77protein CYPRO4-like OS=Cucurbita maxima OX=3661 GN=LOC111482893 PE=4 SV=1[more]
A0A5D3DQK80.089.09Protein CYPRO4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1441G0002... [more]
A0A1S3C2310.089.09protein CYPRO4 OS=Cucumis melo OX=3656 GN=LOC103496156 PE=4 SV=1[more]
A0A0A0LAA10.089.18VID27 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G207380 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT3G19240.11.7e-26972.12Vacuolar import/degradation, Vid27-related protein [more]
AT4G33400.11.4e-23162.33Vacuolar import/degradation, Vid27-related protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013863Vacuolar import/degradation Vid27, C-terminalPFAMPF08553VID27coord: 277..592
e-value: 3.2E-42
score: 144.9
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 342..527
e-value: 7.3E-9
score: 37.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 14..35
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 118..137
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..54
NoneNo IPR availablePANTHERPTHR31913:SF7DEM PROTEINcoord: 1..649
NoneNo IPR availableSUPERFAMILY101908Putative isomerase YbhEcoord: 348..548
IPR040458Vacuolar import/degradation Vid27PANTHERPTHR31913VACUOLAR IMPORT AND DEGRADATION PROTEIN 27coord: 1..649

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g244250.m01Csor.00g244250.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding