Csor.00g243180 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g243180
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description9-cis-epoxycarotenoid dioxygenase
LocationCsor_Chr16: 2350849 .. 2351871 (+)
RNA-Seq ExpressionCsor.00g243180
SyntenyCsor.00g243180
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSsinglestart_codonpolypeptidestop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTCTTGTTTCTTCTTCTTCTTCTTCTTCTTCTTCGTGGGTCAAATCTGGTTTTTCCTCTTCAATGTCGGAGTGTTTCTCTGTAAATATGGATTCCGGCAGGAACAGAGTCGCCTGTTCTCTTCATACTCCTTCCATAATTCAAATTCCTAAGCGTTCCCAGACTGCATTTCGGTCTCCGGTAAAGACCACGGTGCCGAAACCGGTGCCGGTTTCGGCTCCTTCGGTGTCGGAGGAGTGGAATTTCTTGCAGCGGGCTGCGGCGATGGCGCTTGATGCTGTTGAGGATGCTCTGGTTTCGGCGGAGAAGAAGCATTCGTTGCCGAAGACGGCGGACCCTTCTGTTCAAATTGCTGGGAATTTTGCTCCGGTGCCGGAGCAGTCGGTTCGGAAGGGGCTGCCGGTGATTGGGAAAGTTCCGGAGTGTATTCGGGGGGTTTATGTTCGAAACGGCGCGAACCCACTTCATGAACCGGTGTCCGGTCACCACTTGTTTGATGGTGATGGGATGGTTCATGCTGTGGAGTTCACTGAGGACGGCGGCGTTAGTTATGCTTGCCGGTTTACGGAGACTCAACGGCTGGTTCAGGAACGAGCTTATGGCCGGCCGGTTTTTCCGAAAGCCATTGGAGAACTTCATGGCCACTCTGGTATAGCTCGGCTGTTGCTTTTCTATGCGAGAGGTTTATTTGGGCTGGTCGATCACAGCCATGGAATTGGAGTCGCCAACGCCGGTTTGGTTTATTTCAACGGCCAGCTTCTCGCCATGTCGGAGGATGATTTGCCTTACGAAATCAGAGTTACTCCGGCAGGGGATTTGAAAACCGTTGGCCGGTTTGATTTCGACGGCCAATTGAAATCCACAATGATAGCCCACCCAAAACTCGACCCTGTTTCCGGCGAGATGTTTGCTTTAAGCTACGACGTAATCCAAAAGCCATATTTGAAATACTTCAAATTCTCGCCGGAGGGGAAAAGTCACCGGACGTTGAAATTCCCCTGCTTCAGCCAACGATGA

mRNA sequence

ATGGCTTCTCTTGTTTCTTCTTCTTCTTCTTCTTCTTCTTCGTGGGTCAAATCTGGTTTTTCCTCTTCAATGTCGGAGTGTTTCTCTGTAAATATGGATTCCGGCAGGAACAGAGTCGCCTGTTCTCTTCATACTCCTTCCATAATTCAAATTCCTAAGCGTTCCCAGACTGCATTTCGGTCTCCGGTAAAGACCACGGTGCCGAAACCGGTGCCGGTTTCGGCTCCTTCGGTGTCGGAGGAGTGGAATTTCTTGCAGCGGGCTGCGGCGATGGCGCTTGATGCTGTTGAGGATGCTCTGGTTTCGGCGGAGAAGAAGCATTCGTTGCCGAAGACGGCGGACCCTTCTGTTCAAATTGCTGGGAATTTTGCTCCGGTGCCGGAGCAGTCGGTTCGGAAGGGGCTGCCGGTGATTGGGAAAGTTCCGGAGTGTATTCGGGGGGTTTATGTTCGAAACGGCGCGAACCCACTTCATGAACCGGTGTCCGGTCACCACTTGTTTGATGGTGATGGGATGGTTCATGCTGTGGAGTTCACTGAGGACGGCGGCGTTAGTTATGCTTGCCGGTTTACGGAGACTCAACGGCTGGTTCAGGAACGAGCTTATGGCCGGCCGGTTTTTCCGAAAGCCATTGGAGAACTTCATGGCCACTCTGGTATAGCTCGGCTGTTGCTTTTCTATGCGAGAGGTTTATTTGGGCTGGTCGATCACAGCCATGGAATTGGAGTCGCCAACGCCGGTTTGGTTTATTTCAACGGCCAGCTTCTCGCCATGTCGGAGGATGATTTGCCTTACGAAATCAGAGTTACTCCGGCAGGGGATTTGAAAACCGTTGGCCGGTTTGATTTCGACGGCCAATTGAAATCCACAATGATAGCCCACCCAAAACTCGACCCTGTTTCCGGCGAGATGTTTGCTTTAAGCTACGACGTAATCCAAAAGCCATATTTGAAATACTTCAAATTCTCGCCGGAGGGGAAAAGTCACCGGACGTTGAAATTCCCCTGCTTCAGCCAACGATGA

Coding sequence (CDS)

ATGGCTTCTCTTGTTTCTTCTTCTTCTTCTTCTTCTTCTTCGTGGGTCAAATCTGGTTTTTCCTCTTCAATGTCGGAGTGTTTCTCTGTAAATATGGATTCCGGCAGGAACAGAGTCGCCTGTTCTCTTCATACTCCTTCCATAATTCAAATTCCTAAGCGTTCCCAGACTGCATTTCGGTCTCCGGTAAAGACCACGGTGCCGAAACCGGTGCCGGTTTCGGCTCCTTCGGTGTCGGAGGAGTGGAATTTCTTGCAGCGGGCTGCGGCGATGGCGCTTGATGCTGTTGAGGATGCTCTGGTTTCGGCGGAGAAGAAGCATTCGTTGCCGAAGACGGCGGACCCTTCTGTTCAAATTGCTGGGAATTTTGCTCCGGTGCCGGAGCAGTCGGTTCGGAAGGGGCTGCCGGTGATTGGGAAAGTTCCGGAGTGTATTCGGGGGGTTTATGTTCGAAACGGCGCGAACCCACTTCATGAACCGGTGTCCGGTCACCACTTGTTTGATGGTGATGGGATGGTTCATGCTGTGGAGTTCACTGAGGACGGCGGCGTTAGTTATGCTTGCCGGTTTACGGAGACTCAACGGCTGGTTCAGGAACGAGCTTATGGCCGGCCGGTTTTTCCGAAAGCCATTGGAGAACTTCATGGCCACTCTGGTATAGCTCGGCTGTTGCTTTTCTATGCGAGAGGTTTATTTGGGCTGGTCGATCACAGCCATGGAATTGGAGTCGCCAACGCCGGTTTGGTTTATTTCAACGGCCAGCTTCTCGCCATGTCGGAGGATGATTTGCCTTACGAAATCAGAGTTACTCCGGCAGGGGATTTGAAAACCGTTGGCCGGTTTGATTTCGACGGCCAATTGAAATCCACAATGATAGCCCACCCAAAACTCGACCCTGTTTCCGGCGAGATGTTTGCTTTAAGCTACGACGTAATCCAAAAGCCATATTTGAAATACTTCAAATTCTCGCCGGAGGGGAAAAGTCACCGGACGTTGAAATTCCCCTGCTTCAGCCAACGATGA

Protein sequence

MASLVSSSSSSSSSWVKSGFSSSMSECFSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFRSPVKTTVPKPVPVSAPSVSEEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIAGNFAPVPEQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGKSHRTLKFPCFSQR
Homology
BLAST of Csor.00g243180 vs. ExPASy Swiss-Prot
Match: O24023 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED1 PE=1 SV=2)

HSP 1 Score: 425.6 bits (1093), Expect = 5.2e-118
Identity = 218/345 (63.19%), Postives = 264/345 (76.52%), Query Frame = 0

Query: 4   LVSSSSSSSSSWVKSGFSSSMSECFSVNMDS------GRNR----VACSLHTPSIIQIPK 63
           + +++S ++++W+K+  S   S+ F    +S        NR    +  SL  P I+  PK
Sbjct: 1   MATTTSHATNTWIKTKLSMPSSKEFGFASNSISLLKNQHNRQSLNINSSLQAPPILHFPK 60

Query: 64  RSQTAFRSPVKTTVPKPVPV---SAPSVSEEWNFLQRAAAMALDAVEDALVSAEKKHSLP 123
           +S   +++P   T+  P      S+ S + +WN +Q+AAAMALDAVE AL   E +H LP
Sbjct: 61  QSSN-YQTPKNNTISHPKQENNNSSSSSTSKWNLVQKAAAMALDAVESALTKHELEHPLP 120

Query: 124 KTADPSVQIAGNFAPVPEQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGD 183
           KTADP VQI+GNFAPVPE  V + LPV GK+P+C++GVYVRNGANPL EP +GHH FDGD
Sbjct: 121 KTADPRVQISGNFAPVPENPVCQSLPVTGKIPKCVQGVYVRNGANPLFEPTAGHHFFDGD 180

Query: 184 GMVHAVEFTEDGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARG 243
           GMVHAV+F ++G  SYACRFTET+RLVQE+A GRPVFPKAIGELHGHSGIARL+LFYARG
Sbjct: 181 GMVHAVQF-KNGSASYACRFTETERLVQEKALGRPVFPKAIGELHGHSGIARLMLFYARG 240

Query: 244 LFGLVDHSHGIGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKST 303
           LFGLVDHS G GVANAGLVYFN +LLAMSEDDLPY ++VTP GDLKT GRFDFDGQLKST
Sbjct: 241 LFGLVDHSKGTGVANAGLVYFNNRLLAMSEDDLPYHVKVTPTGDLKTEGRFDFDGQLKST 300

Query: 304 MIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGKSHRTLKFP 336
           MIAHPKLDPVSGE+FALSYDVIQKPYLKYF+FS  G+    ++ P
Sbjct: 301 MIAHPKLDPVSGELFALSYDVIQKPYLKYFRFSKNGEKSNDVEIP 343

BLAST of Csor.00g243180 vs. ExPASy Swiss-Prot
Match: Q9LRR7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED3 PE=1 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 6.3e-116
Identity = 213/314 (67.83%), Postives = 247/314 (78.66%), Query Frame = 0

Query: 16  VKSGFSSSMSECFSVNMDSGRNR---VACSLHTPSIIQIPKRSQTAFRSPVKTTVPKPVP 75
           + S  SS +S C S+ M S   R   V+ +LHTP  +  PK+S     SP     PK   
Sbjct: 24  LSSSQSSDLSYCSSLPMASRVTRKLNVSSALHTPPALHFPKQSS---NSPAIVVKPK--- 83

Query: 76  VSAPSVSEEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIAGNFAPVPEQSVR 135
            +  S +++ N  QRAAA ALDA E  LVS EK H LPKTADPSVQIAGNFAPV EQ VR
Sbjct: 84  -AKESNTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQIAGNFAPVNEQPVR 143

Query: 136 KGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYACRFTE 195
           + LPV+GK+P+ I+GVYVRNGANPLHEPV+GHH FDGDGMVHAV+F E G  SYACRFT+
Sbjct: 144 RNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKF-EHGSASYACRFTQ 203

Query: 196 TQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFN 255
           T R VQER  GRPVFPKAIGELHGH+GIARL+LFYAR   G+VD +HG GVANAGLVYFN
Sbjct: 204 TNRFVQERQLGRPVFPKAIGELHGHTGIARLMLFYARAAAGIVDPAHGTGVANAGLVYFN 263

Query: 256 GQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVI 315
           G+LLAMSEDDLPY++++TP GDLKTVGRFDFDGQL+STMIAHPK+DP SGE+FALSYDV+
Sbjct: 264 GRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVDPESGELFALSYDVV 323

Query: 316 QKPYLKYFKFSPEG 327
            KPYLKYF+FSP+G
Sbjct: 324 SKPYLKYFRFSPDG 329

BLAST of Csor.00g243180 vs. ExPASy Swiss-Prot
Match: Q9M6E8 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=3885 GN=NCED1 PE=2 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 2.9e-113
Identity = 216/346 (62.43%), Postives = 251/346 (72.54%), Query Frame = 0

Query: 3   SLVSSSSSSSSSWVKSGFSSSMSECFSVNMDSGRNRVACS---LHTPSIIQIPKRSQTAF 62
           +L SS SS       +  S +    F     S  N + CS   LH P   Q    S T  
Sbjct: 13  TLPSSCSSPFKDLASTSSSPTTLLPFKKRSSSNTNTITCSLQTLHYPKQYQPTSTSTTTT 72

Query: 63  RSPVK------TTVP----KPVPVSAPSVSEEWNFLQRAAAMALDAVEDALVSAEKKHSL 122
            +P+K      TT P    KP+  +     ++WNFLQ+AAA  LD VE ALVS E KH L
Sbjct: 73  PTPIKPTTTTTTTTPHRETKPLSDTKQPFPQKWNFLQKAAATGLDMVETALVSHESKHPL 132

Query: 123 PKTADPSVQIAGNFAPVPEQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDG 182
           PKTADP VQIAGNFAPVPE +  + LPV+GK+P+CI GVYVRNGANPL+EPV+GHH FDG
Sbjct: 133 PKTADPKVQIAGNFAPVPEHAADQALPVVGKIPKCIDGVYVRNGANPLYEPVAGHHFFDG 192

Query: 183 DGMVHAVEFTEDGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYAR 242
           DGMVHAV+FT +G  SYACRFTETQRL QE++ GRPVFPKAIGELHGHSGIARLLLFYAR
Sbjct: 193 DGMVHAVKFT-NGAASYACRFTETQRLAQEKSLGRPVFPKAIGELHGHSGIARLLLFYAR 252

Query: 243 GLFGLVDHSHGIGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKS 302
            LF LVD SHG+GVANAGLVYFN  LLAMSEDDLPY +R+T  GDL TVGR+DF+GQL S
Sbjct: 253 SLFQLVDGSHGMGVANAGLVYFNNHLLAMSEDDLPYHVRITSNGDLTTVGRYDFNGQLNS 312

Query: 303 TMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGKSHRTLKFP 336
           TMIAHPKLDPV+G++ ALSYDV+QKPYLKYF+FS +G     ++ P
Sbjct: 313 TMIAHPKLDPVNGDLHALSYDVVQKPYLKYFRFSADGVKSPDVEIP 357

BLAST of Csor.00g243180 vs. ExPASy Swiss-Prot
Match: K4CJJ1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED2 PE=2 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 3.2e-112
Identity = 212/328 (64.63%), Postives = 250/328 (76.22%), Query Frame = 0

Query: 8   SSSSSSSWVKSGFSSSMSECFSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFRSPVKTTV 67
           +S+ ++  V   FS S S  +S++       ++   HT +  +I     T    P +   
Sbjct: 2   TSTIANYRVSHSFSPSTS--YSLDFTLPSKSISMKNHTTTKTKIHSALLTL---PKQNNT 61

Query: 68  PKPVPVSAPSVSEEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIAGNFAPVP 127
           PK  P    S    WNF Q+AAA ALD VE ALVS E ++ LPKTADP VQIAGNFAPVP
Sbjct: 62  PKNQPQFQTS---HWNFFQKAAAKALDIVESALVSRELQNPLPKTADPRVQIAGNFAPVP 121

Query: 128 EQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYA 187
           EQSVR  LPV G +P+CI GVYVRNGANPL EPV+GHHLFDGDGMVHAV   E+G VSY+
Sbjct: 122 EQSVRHNLPVTGTIPDCINGVYVRNGANPLFEPVAGHHLFDGDGMVHAVT-VENGSVSYS 181

Query: 188 CRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAG 247
           CRFTET+RLVQER  G PVFPKAIGELHGHSGIARLLLFYARG+FGLVDHSHG GVANAG
Sbjct: 182 CRFTETERLVQERELGHPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHSHGTGVANAG 241

Query: 248 LVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFAL 307
           LV+FN +LLAMSEDD+PY ++V P+GDL+TVGR++FD QLKSTMIAHPK+DPVSGE+FAL
Sbjct: 242 LVFFNNRLLAMSEDDVPYHVQVLPSGDLQTVGRYNFDDQLKSTMIAHPKIDPVSGELFAL 301

Query: 308 SYDVIQKPYLKYFKFSPEGKSHRTLKFP 336
           SYDV+QKPYLK FKFSP+G+    ++ P
Sbjct: 302 SYDVVQKPYLKSFKFSPDGEKSPDVEIP 320

BLAST of Csor.00g243180 vs. ExPASy Swiss-Prot
Match: Q9C6Z1 (Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED5 PE=1 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 1.4e-107
Identity = 186/271 (68.63%), Postives = 221/271 (81.55%), Query Frame = 0

Query: 68  PKPVPVSAPSVSEEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIAGNFAPVP 127
           P P P+     +  WN LQRAA+ ALD  E AL+  E+   LPKT DP  QI+GN+APVP
Sbjct: 62  PSPNPIIPEKDTSRWNPLQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVP 121

Query: 128 EQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYA 187
           EQSV+  L V GK+P+CI GVY+RNGANPL EPVSGHHLFDGDGMVHAV+ T +G  SY+
Sbjct: 122 EQSVKSSLSVDGKIPDCIDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKIT-NGDASYS 181

Query: 188 CRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAG 247
           CRFTET+RLVQE+  G P+FPKAIGELHGHSGIARL+LFYARGLFGL++H +G GVANAG
Sbjct: 182 CRFTETERLVQEKQLGSPIFPKAIGELHGHSGIARLMLFYARGLFGLLNHKNGTGVANAG 241

Query: 248 LVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFAL 307
           LVYF+ +LLAMSEDDLPY++RVT  GDL+T+GRFDFDGQL S MIAHPK+DPV+ E+FAL
Sbjct: 242 LVYFHDRLLAMSEDDLPYQVRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFAL 301

Query: 308 SYDVIQKPYLKYFKFSPEGKSHRTLKFPCFS 339
           SYDV++KPYLKYFKFSPEG+    ++ P  S
Sbjct: 302 SYDVVKKPYLKYFKFSPEGEKSPDVEIPLAS 331

BLAST of Csor.00g243180 vs. NCBI nr
Match: KAG6577061.1 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 668 bits (1724), Expect = 5.07e-242
Identity = 340/340 (100.00%), Postives = 340/340 (100.00%), Query Frame = 0

Query: 1   MASLVSSSSSSSSSWVKSGFSSSMSECFSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFR 60
           MASLVSSSSSSSSSWVKSGFSSSMSECFSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFR
Sbjct: 1   MASLVSSSSSSSSSWVKSGFSSSMSECFSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFR 60

Query: 61  SPVKTTVPKPVPVSAPSVSEEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIA 120
           SPVKTTVPKPVPVSAPSVSEEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIA
Sbjct: 61  SPVKTTVPKPVPVSAPSVSEEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIA 120

Query: 121 GNFAPVPEQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTE 180
           GNFAPVPEQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTE
Sbjct: 121 GNFAPVPEQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTE 180

Query: 181 DGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHG 240
           DGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHG
Sbjct: 181 DGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHG 240

Query: 241 IGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPV 300
           IGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPV
Sbjct: 241 IGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPV 300

Query: 301 SGEMFALSYDVIQKPYLKYFKFSPEGKSHRTLKFPCFSQR 340
           SGEMFALSYDVIQKPYLKYFKFSPEGKSHRTLKFPCFSQR
Sbjct: 301 SGEMFALSYDVIQKPYLKYFKFSPEGKSHRTLKFPCFSQR 340

BLAST of Csor.00g243180 vs. NCBI nr
Match: XP_022931264.1 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 633 bits (1632), Expect = 7.40e-224
Identity = 324/335 (96.72%), Postives = 327/335 (97.61%), Query Frame = 0

Query: 1   MASLVSSSSSSSSSWVKSGFSSSMSECFSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFR 60
           MASLVSSSSSS   WVKSGFSSSMSECFSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFR
Sbjct: 1   MASLVSSSSSS---WVKSGFSSSMSECFSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFR 60

Query: 61  SPVKTTVPKPVPVSAPSVSEEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIA 120
           SPVKTTVPKPVPVSAPSVSEEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIA
Sbjct: 61  SPVKTTVPKPVPVSAPSVSEEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIA 120

Query: 121 GNFAPVPEQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTE 180
           GNFAPVPEQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTE
Sbjct: 121 GNFAPVPEQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTE 180

Query: 181 DGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHG 240
           DGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHG
Sbjct: 181 DGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHG 240

Query: 241 IGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPV 300
           IGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPV
Sbjct: 241 IGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPV 300

Query: 301 SGEMFALSYDVIQKPYLKYFKFSPEGKSHRTLKFP 335
           SGEMFALSYDVIQKPYLKYFKFSPEG+    ++ P
Sbjct: 301 SGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIP 332

BLAST of Csor.00g243180 vs. NCBI nr
Match: XP_023551972.1 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 618 bits (1594), Expect = 2.71e-218
Identity = 312/324 (96.30%), Postives = 316/324 (97.53%), Query Frame = 0

Query: 12  SSSWVKSGFSSSMSECFSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFRSPVKTTVPKPV 71
           SSSWVKSGFSSSMSE FSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFRSPVKTTVPKPV
Sbjct: 6   SSSWVKSGFSSSMSERFSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFRSPVKTTVPKPV 65

Query: 72  PVSAPSVSEEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIAGNFAPVPEQSV 131
           PVS PSVSEEWNFLQRAAAMALDAVEDAL+SAEKKHSLPKTADPSVQIAGNFAPV EQSV
Sbjct: 66  PVSPPSVSEEWNFLQRAAAMALDAVEDALLSAEKKHSLPKTADPSVQIAGNFAPVTEQSV 125

Query: 132 RKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYACRFT 191
           RKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYACRFT
Sbjct: 126 RKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYACRFT 185

Query: 192 ETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYF 251
           ETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYF
Sbjct: 186 ETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYF 245

Query: 252 NGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDV 311
           NGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDV
Sbjct: 246 NGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDV 305

Query: 312 IQKPYLKYFKFSPEGKSHRTLKFP 335
           IQKPYLKYFKFSPEG+    ++ P
Sbjct: 306 IQKPYLKYFKFSPEGEKSPDVEIP 329

BLAST of Csor.00g243180 vs. NCBI nr
Match: XP_022985159.1 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 612 bits (1577), Expect = 1.19e-215
Identity = 315/335 (94.03%), Postives = 320/335 (95.52%), Query Frame = 0

Query: 1   MASLVSSSSSSSSSWVKSGFSSSMSECFSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFR 60
           MASL SSSSSSS  WVKSGFSS MSE FSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFR
Sbjct: 1   MASLASSSSSSS--WVKSGFSSLMSERFSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFR 60

Query: 61  SPVKTTVPKPVPVSAPSVSEEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIA 120
           SPVKTTVPKPVPV APSVSEEWNFLQRAAAMALDAVEDALVS E+KHSLPKTADPSVQIA
Sbjct: 61  SPVKTTVPKPVPVPAPSVSEEWNFLQRAAAMALDAVEDALVSVERKHSLPKTADPSVQIA 120

Query: 121 GNFAPVPEQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTE 180
           GNFAPVPEQ VRKGLPVIGKVPE IRG+YVRNGANPLHEPVSGHHLFDGDGMVHAVEFTE
Sbjct: 121 GNFAPVPEQPVRKGLPVIGKVPESIRGIYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTE 180

Query: 181 DGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHG 240
           DG VSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHG
Sbjct: 181 DGEVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHG 240

Query: 241 IGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPV 300
           IGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPV
Sbjct: 241 IGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPV 300

Query: 301 SGEMFALSYDVIQKPYLKYFKFSPEGKSHRTLKFP 335
           SGEMFALSYDVIQKPYLKYFKFSPEG+    ++ P
Sbjct: 301 SGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIP 333

BLAST of Csor.00g243180 vs. NCBI nr
Match: APO14273.1 (9-cis-epoxycarotenoid dioxygenase NCED1 [Luffa aegyptiaca])

HSP 1 Score: 575 bits (1483), Expect = 2.40e-201
Identity = 294/330 (89.09%), Postives = 308/330 (93.33%), Query Frame = 0

Query: 10  SSSSSWVKSGFSSSMSECFSVNMDSG-RNRVACSLHTPSIIQIPKRSQTAFRSPVKTTVP 69
           SSSSSWVKS FSSSM E  SVN + G +NRVACSLHTPSIIQIPK SQTAFRSPVKT VP
Sbjct: 6   SSSSSWVKSRFSSSMPELVSVNRNYGLQNRVACSLHTPSIIQIPKHSQTAFRSPVKTAVP 65

Query: 70  KPVPVSAPSVS---EEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIAGNFAP 129
           KPVPVS PSVS   EEWNFLQRAAA+ALDAVEDALVSAE+KHSLPKTADP+VQIAGNFAP
Sbjct: 66  KPVPVSPPSVSSGSEEWNFLQRAAAVALDAVEDALVSAERKHSLPKTADPAVQIAGNFAP 125

Query: 130 VPEQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVS 189
           VPEQ VRK LPV GKVP+CIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEF+E GGVS
Sbjct: 126 VPEQPVRKALPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEGGGVS 185

Query: 190 YACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVAN 249
           YACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDHSHG+GVAN
Sbjct: 186 YACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGLGVAN 245

Query: 250 AGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMF 309
           AGLVYFNG+LLAMSEDDLPY+IRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMF
Sbjct: 246 AGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMF 305

Query: 310 ALSYDVIQKPYLKYFKFSPEGKSHRTLKFP 335
           ALSYDVIQKPYLKYFKFSPEG+    ++ P
Sbjct: 306 ALSYDVIQKPYLKYFKFSPEGEKSPDVEIP 335

BLAST of Csor.00g243180 vs. ExPASy TrEMBL
Match: A0A6J1ETU1 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111437497 PE=3 SV=1)

HSP 1 Score: 633 bits (1632), Expect = 3.58e-224
Identity = 324/335 (96.72%), Postives = 327/335 (97.61%), Query Frame = 0

Query: 1   MASLVSSSSSSSSSWVKSGFSSSMSECFSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFR 60
           MASLVSSSSSS   WVKSGFSSSMSECFSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFR
Sbjct: 1   MASLVSSSSSS---WVKSGFSSSMSECFSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFR 60

Query: 61  SPVKTTVPKPVPVSAPSVSEEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIA 120
           SPVKTTVPKPVPVSAPSVSEEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIA
Sbjct: 61  SPVKTTVPKPVPVSAPSVSEEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIA 120

Query: 121 GNFAPVPEQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTE 180
           GNFAPVPEQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTE
Sbjct: 121 GNFAPVPEQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTE 180

Query: 181 DGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHG 240
           DGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHG
Sbjct: 181 DGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHG 240

Query: 241 IGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPV 300
           IGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPV
Sbjct: 241 IGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPV 300

Query: 301 SGEMFALSYDVIQKPYLKYFKFSPEGKSHRTLKFP 335
           SGEMFALSYDVIQKPYLKYFKFSPEG+    ++ P
Sbjct: 301 SGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIP 332

BLAST of Csor.00g243180 vs. ExPASy TrEMBL
Match: A0A6J1JCI7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111483246 PE=3 SV=1)

HSP 1 Score: 612 bits (1577), Expect = 5.76e-216
Identity = 315/335 (94.03%), Postives = 320/335 (95.52%), Query Frame = 0

Query: 1   MASLVSSSSSSSSSWVKSGFSSSMSECFSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFR 60
           MASL SSSSSSS  WVKSGFSS MSE FSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFR
Sbjct: 1   MASLASSSSSSS--WVKSGFSSLMSERFSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFR 60

Query: 61  SPVKTTVPKPVPVSAPSVSEEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIA 120
           SPVKTTVPKPVPV APSVSEEWNFLQRAAAMALDAVEDALVS E+KHSLPKTADPSVQIA
Sbjct: 61  SPVKTTVPKPVPVPAPSVSEEWNFLQRAAAMALDAVEDALVSVERKHSLPKTADPSVQIA 120

Query: 121 GNFAPVPEQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTE 180
           GNFAPVPEQ VRKGLPVIGKVPE IRG+YVRNGANPLHEPVSGHHLFDGDGMVHAVEFTE
Sbjct: 121 GNFAPVPEQPVRKGLPVIGKVPESIRGIYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTE 180

Query: 181 DGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHG 240
           DG VSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHG
Sbjct: 181 DGEVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHG 240

Query: 241 IGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPV 300
           IGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPV
Sbjct: 241 IGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPV 300

Query: 301 SGEMFALSYDVIQKPYLKYFKFSPEGKSHRTLKFP 335
           SGEMFALSYDVIQKPYLKYFKFSPEG+    ++ P
Sbjct: 301 SGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIP 333

BLAST of Csor.00g243180 vs. ExPASy TrEMBL
Match: A0A1L5JHV0 (9-cis-epoxycarotenoid dioxygenase NCED1 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 575 bits (1483), Expect = 1.16e-201
Identity = 294/330 (89.09%), Postives = 308/330 (93.33%), Query Frame = 0

Query: 10  SSSSSWVKSGFSSSMSECFSVNMDSG-RNRVACSLHTPSIIQIPKRSQTAFRSPVKTTVP 69
           SSSSSWVKS FSSSM E  SVN + G +NRVACSLHTPSIIQIPK SQTAFRSPVKT VP
Sbjct: 6   SSSSSWVKSRFSSSMPELVSVNRNYGLQNRVACSLHTPSIIQIPKHSQTAFRSPVKTAVP 65

Query: 70  KPVPVSAPSVS---EEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIAGNFAP 129
           KPVPVS PSVS   EEWNFLQRAAA+ALDAVEDALVSAE+KHSLPKTADP+VQIAGNFAP
Sbjct: 66  KPVPVSPPSVSSGSEEWNFLQRAAAVALDAVEDALVSAERKHSLPKTADPAVQIAGNFAP 125

Query: 130 VPEQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVS 189
           VPEQ VRK LPV GKVP+CIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEF+E GGVS
Sbjct: 126 VPEQPVRKALPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEGGGVS 185

Query: 190 YACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVAN 249
           YACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDHSHG+GVAN
Sbjct: 186 YACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGLGVAN 245

Query: 250 AGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMF 309
           AGLVYFNG+LLAMSEDDLPY+IRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMF
Sbjct: 246 AGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMF 305

Query: 310 ALSYDVIQKPYLKYFKFSPEGKSHRTLKFP 335
           ALSYDVIQKPYLKYFKFSPEG+    ++ P
Sbjct: 306 ALSYDVIQKPYLKYFKFSPEGEKSPDVEIP 335

BLAST of Csor.00g243180 vs. ExPASy TrEMBL
Match: A0A6J1FPE2 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111447037 PE=3 SV=1)

HSP 1 Score: 549 bits (1415), Expect = 1.57e-191
Identity = 279/319 (87.46%), Postives = 295/319 (92.48%), Query Frame = 0

Query: 21  SSSMSECFSVNMDSG-RNRVACSLHTPSIIQIPKRSQTAFRSPVKTTVPKPVPVSAPSVS 80
           SSSM +  SVN + G RNRV+CSLHTPSIIQIPKRSQTAFRSP+K  VPKP PV+APSVS
Sbjct: 4   SSSMPKILSVNRNYGVRNRVSCSLHTPSIIQIPKRSQTAFRSPIKIAVPKPEPVAAPSVS 63

Query: 81  ---EEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIAGNFAPVPEQSVRKGLP 140
              +EWNFLQ+AAAMALDAVEDALVS EKKHSLPKTADPSVQIAGNFAPVPEQ VRK LP
Sbjct: 64  SPAKEWNFLQQAAAMALDAVEDALVSMEKKHSLPKTADPSVQIAGNFAPVPEQPVRKVLP 123

Query: 141 VIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYACRFTETQRL 200
           V GKVP+CIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTE G VSYACRFTET+RL
Sbjct: 124 VTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTECGRVSYACRFTETERL 183

Query: 201 VQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGQLL 260
           VQERAYGRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDHSHGIGVANAGLVYFNG+LL
Sbjct: 184 VQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIGVANAGLVYFNGRLL 243

Query: 261 AMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPY 320
           AMSEDDLPY+IRVTP+GDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPY
Sbjct: 244 AMSEDDLPYQIRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPY 303

Query: 321 LKYFKFSPEGKSHRTLKFP 335
           LKYFKFS EG+    ++ P
Sbjct: 304 LKYFKFSAEGEKSPDVEIP 322

BLAST of Csor.00g243180 vs. ExPASy TrEMBL
Match: A0A346DA07 (9-cis-epoxycarotenoid dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111479551 PE=2 SV=1)

HSP 1 Score: 536 bits (1381), Expect = 1.98e-186
Identity = 271/318 (85.22%), Postives = 291/318 (91.51%), Query Frame = 0

Query: 21  SSSMSECFSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFRSPVKTTVPKPVPVSAPSVS- 80
           SSSM +   +N    RNR++CSLHTPSIIQIPKRSQTAFRSP+K  +PKP  V+APSVS 
Sbjct: 5   SSSMPKILPIN----RNRLSCSLHTPSIIQIPKRSQTAFRSPIKIAMPKPELVAAPSVSS 64

Query: 81  --EEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIAGNFAPVPEQSVRKGLPV 140
             +EWNFLQ+AAA+ALDAVEDALVS E+KHSLPKTADPSVQIAGNFAPVPEQ VRK LPV
Sbjct: 65  PTKEWNFLQQAAALALDAVEDALVSMERKHSLPKTADPSVQIAGNFAPVPEQPVRKVLPV 124

Query: 141 IGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYACRFTETQRLV 200
            GKVP+CIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTE G VSYACRFTET+RLV
Sbjct: 125 TGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTECGRVSYACRFTETERLV 184

Query: 201 QERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGQLLA 260
           QERAYGRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDHSHGIGVANAGLVYFNG+LLA
Sbjct: 185 QERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIGVANAGLVYFNGRLLA 244

Query: 261 MSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYL 320
           MSEDDLPY+IRVTP+GDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYL
Sbjct: 245 MSEDDLPYQIRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYL 304

Query: 321 KYFKFSPEGKSHRTLKFP 335
           KYFKFS EG+    ++ P
Sbjct: 305 KYFKFSAEGEKSPDVEIP 318

BLAST of Csor.00g243180 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 418.7 bits (1075), Expect = 4.5e-117
Identity = 213/314 (67.83%), Postives = 247/314 (78.66%), Query Frame = 0

Query: 16  VKSGFSSSMSECFSVNMDSGRNR---VACSLHTPSIIQIPKRSQTAFRSPVKTTVPKPVP 75
           + S  SS +S C S+ M S   R   V+ +LHTP  +  PK+S     SP     PK   
Sbjct: 24  LSSSQSSDLSYCSSLPMASRVTRKLNVSSALHTPPALHFPKQSS---NSPAIVVKPK--- 83

Query: 76  VSAPSVSEEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIAGNFAPVPEQSVR 135
            +  S +++ N  QRAAA ALDA E  LVS EK H LPKTADPSVQIAGNFAPV EQ VR
Sbjct: 84  -AKESNTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQIAGNFAPVNEQPVR 143

Query: 136 KGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYACRFTE 195
           + LPV+GK+P+ I+GVYVRNGANPLHEPV+GHH FDGDGMVHAV+F E G  SYACRFT+
Sbjct: 144 RNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKF-EHGSASYACRFTQ 203

Query: 196 TQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFN 255
           T R VQER  GRPVFPKAIGELHGH+GIARL+LFYAR   G+VD +HG GVANAGLVYFN
Sbjct: 204 TNRFVQERQLGRPVFPKAIGELHGHTGIARLMLFYARAAAGIVDPAHGTGVANAGLVYFN 263

Query: 256 GQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVI 315
           G+LLAMSEDDLPY++++TP GDLKTVGRFDFDGQL+STMIAHPK+DP SGE+FALSYDV+
Sbjct: 264 GRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVDPESGELFALSYDVV 323

Query: 316 QKPYLKYFKFSPEG 327
            KPYLKYF+FSP+G
Sbjct: 324 SKPYLKYFRFSPDG 329

BLAST of Csor.00g243180 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 391.0 bits (1003), Expect = 1.0e-108
Identity = 186/271 (68.63%), Postives = 221/271 (81.55%), Query Frame = 0

Query: 68  PKPVPVSAPSVSEEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIAGNFAPVP 127
           P P P+     +  WN LQRAA+ ALD  E AL+  E+   LPKT DP  QI+GN+APVP
Sbjct: 62  PSPNPIIPEKDTSRWNPLQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVP 121

Query: 128 EQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYA 187
           EQSV+  L V GK+P+CI GVY+RNGANPL EPVSGHHLFDGDGMVHAV+ T +G  SY+
Sbjct: 122 EQSVKSSLSVDGKIPDCIDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKIT-NGDASYS 181

Query: 188 CRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAG 247
           CRFTET+RLVQE+  G P+FPKAIGELHGHSGIARL+LFYARGLFGL++H +G GVANAG
Sbjct: 182 CRFTETERLVQEKQLGSPIFPKAIGELHGHSGIARLMLFYARGLFGLLNHKNGTGVANAG 241

Query: 248 LVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFAL 307
           LVYF+ +LLAMSEDDLPY++RVT  GDL+T+GRFDFDGQL S MIAHPK+DPV+ E+FAL
Sbjct: 242 LVYFHDRLLAMSEDDLPYQVRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFAL 301

Query: 308 SYDVIQKPYLKYFKFSPEGKSHRTLKFPCFS 339
           SYDV++KPYLKYFKFSPEG+    ++ P  S
Sbjct: 302 SYDVVKKPYLKYFKFSPEGEKSPDVEIPLAS 331

BLAST of Csor.00g243180 vs. TAIR 10
Match: AT4G18350.1 (nine-cis-epoxycarotenoid dioxygenase 2 )

HSP 1 Score: 382.1 bits (980), Expect = 4.7e-106
Identity = 178/253 (70.36%), Postives = 216/253 (85.38%), Query Frame = 0

Query: 83  NFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIAGNFAPVPEQSVRKGLPVIGKVP 142
           N  Q+AAA+A+DA E AL+S E+   LPKTADP VQIAGN++PVPE SVR+ L V G +P
Sbjct: 70  NIFQKAAAIAIDAAERALISHEQDSPLPKTADPRVQIAGNYSPVPESSVRRNLTVEGTIP 129

Query: 143 ECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYACRFTETQRLVQERAY 202
           +CI GVY+RNGANP+ EP +GHHLFDGDGMVHAV+ T +G  SYACRFT+T+RLVQE+  
Sbjct: 130 DCIDGVYIRNGANPMFEPTAGHHLFDGDGMVHAVKIT-NGSASYACRFTKTERLVQEKRL 189

Query: 203 GRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGQLLAMSEDD 262
           GRPVFPKAIGELHGHSGIARL+LFYARGL GL+++ +G+GVANAGLVYFN +LLAMSEDD
Sbjct: 190 GRPVFPKAIGELHGHSGIARLMLFYARGLCGLINNQNGVGVANAGLVYFNNRLLAMSEDD 249

Query: 263 LPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKF 322
           LPY++++T  GDL+TVGR+DFDGQLKS MIAHPKLDPV+ E+ ALSYDV++KPYLKYF+F
Sbjct: 250 LPYQLKITQTGDLQTVGRYDFDGQLKSAMIAHPKLDPVTKELHALSYDVVKKPYLKYFRF 309

Query: 323 SPEGKSHRTLKFP 336
           SP+G     L+ P
Sbjct: 310 SPDGVKSPELEIP 321

BLAST of Csor.00g243180 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 378.6 bits (971), Expect = 5.1e-105
Identity = 204/338 (60.36%), Postives = 252/338 (74.56%), Query Frame = 0

Query: 6   SSSSSSSSSWVKS-GFSSSMSE------CFSVNMDSGRNRVACSLHTPSIIQIPKRSQTA 65
           S+SSSSS   ++S  FSS++        C+  +  + ++ V+ SL +P+       S   
Sbjct: 70  STSSSSSRPKLQSLSFSSTLRNKKLVVPCYVSSSVNKKSSVSSSLQSPT---FKPPSWKK 129

Query: 66  FRSPVKTTVPKPVPVSAPSVSEEWNFLQRAAAMALDAVEDALVSAE-KKHSLPKTADPSV 125
             + V   +PK       + + + N +QR AAM LDAVE+A++S E ++H  PKTADP+V
Sbjct: 130 LCNDVTNLIPK-----TTNQNPKLNPVQRTAAMVLDAVENAMISHERRRHPHPKTADPAV 189

Query: 126 QIAGNFAPVPEQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVE 185
           QIAGNF PVPE+ V   LPV G VPECI+GVYVRNGANPLH+PVSGHHLFDGDGMVHAV 
Sbjct: 190 QIAGNFFPVPEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLFDGDGMVHAVR 249

Query: 186 FTEDGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDH 245
           F ++G VSYACRFTET RLVQER  GRPVFPKAIGELHGH GIA+L+LF  RGLFGLVD 
Sbjct: 250 F-DNGSVSYACRFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNTRGLFGLVDP 309

Query: 246 SHGIGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKL 305
           + G+GVANAGLVYFNG LLAMSEDDLPY ++VT  GDL+T GR+DFDGQLKSTMIAHPK+
Sbjct: 310 TGGLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTMIAHPKI 369

Query: 306 DPVSGEMFALSYDVIQKPYLKYFKFSPEGKSHRTLKFP 336
           DP + E+FALSYDV+ KPYLKYF+F+ +G+    ++ P
Sbjct: 370 DPETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEIP 398

BLAST of Csor.00g243180 vs. TAIR 10
Match: AT3G24220.1 (nine-cis-epoxycarotenoid dioxygenase 6 )

HSP 1 Score: 323.6 bits (828), Expect = 2.0e-88
Identity = 152/269 (56.51%), Postives = 201/269 (74.72%), Query Frame = 0

Query: 66  TVPKPVPVSAPSVSEEWNFLQRAAAMALDAVEDALV-SAEKKHSLPKTADPSVQIAGNFA 125
           T P P PV         N LQ+ AA  LD +E ++V   E+   LPK  DP+VQ++GNFA
Sbjct: 47  TSPVPSPVKLKPTYPNLNLLQKLAATMLDKIESSIVIPMEQNRPLPKPTDPAVQLSGNFA 106

Query: 126 PVPEQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGV 185
           PV E  V+ GL V+G++P C++GVY+RNGANP+  P++GHHLFDGDGM+HAV    D  V
Sbjct: 107 PVNECPVQNGLEVVGQIPSCLKGVYIRNGANPMFPPLAGHHLFDGDGMIHAVSIGFDNQV 166

Query: 186 SYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVA 245
           SY+CR+T+T RLVQE A GR VFPK IGELHGHSG+ARL LF AR   GLVD + G+GVA
Sbjct: 167 SYSCRYTKTNRLVQETALGRSVFPKPIGELHGHSGLARLALFTARAGIGLVDGTRGMGVA 226

Query: 246 NAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEM 305
           NAG+V+FNG+LLAMSEDDLPY++++   GDL+T+GRF FD Q+ S++IAHPK+D  +G++
Sbjct: 227 NAGVVFFNGRLLAMSEDDLPYQVKIDGQGDLETIGRFGFDDQIDSSVIAHPKVDATTGDL 286

Query: 306 FALSYDVIQKPYLKYFKFSPEGKSHRTLK 334
             LSY+V++KP+L+Y KF+  GK  R ++
Sbjct: 287 HTLSYNVLKKPHLRYLKFNTCGKKTRDVE 315

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O240235.2e-11863.199-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum O... [more]
Q9LRR76.3e-11667.839-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9M6E82.9e-11362.439-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=... [more]
K4CJJ13.2e-11264.639-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum O... [more]
Q9C6Z11.4e-10768.63Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis t... [more]
Match NameE-valueIdentityDescription
KAG6577061.15.07e-242100.009-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic, partial [Cucurbita argyr... [more]
XP_022931264.17.40e-22496.729-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucurbita moschata][more]
XP_023551972.12.71e-21896.309-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic-like [Cucurbita pepo subs... [more]
XP_022985159.11.19e-21594.039-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucurbita maxima][more]
APO14273.12.40e-20189.099-cis-epoxycarotenoid dioxygenase NCED1 [Luffa aegyptiaca][more]
Match NameE-valueIdentityDescription
A0A6J1ETU13.58e-22496.729-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschat... [more]
A0A6J1JCI75.76e-21694.039-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita maxima ... [more]
A0A1L5JHV01.16e-20189.099-cis-epoxycarotenoid dioxygenase NCED1 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1[more]
A0A6J1FPE21.57e-19187.469-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschat... [more]
A0A346DA071.98e-18685.229-cis-epoxycarotenoid dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111479551 PE... [more]
Match NameE-valueIdentityDescription
AT3G14440.14.5e-11767.83nine-cis-epoxycarotenoid dioxygenase 3 [more]
AT1G30100.11.0e-10868.63nine-cis-epoxycarotenoid dioxygenase 5 [more]
AT4G18350.14.7e-10670.36nine-cis-epoxycarotenoid dioxygenase 2 [more]
AT1G78390.15.1e-10560.36nine-cis-epoxycarotenoid dioxygenase 9 [more]
AT3G24220.12.0e-8856.51nine-cis-epoxycarotenoid dioxygenase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004294Carotenoid oxygenasePFAMPF03055RPE65coord: 123..332
e-value: 2.6E-46
score: 158.7
IPR004294Carotenoid oxygenasePANTHERPTHR10543BETA-CAROTENE DIOXYGENASEcoord: 69..332
NoneNo IPR availablePANTHERPTHR10543:SF1239-CIS-EPOXYCAROTENOID DIOXYGENASE NCED5, CHLOROPLASTIC-RELATEDcoord: 69..332

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g243180.m03Csor.00g243180.m03mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010162 seed dormancy process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0045549 9-cis-epoxycarotenoid dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen