Csor.00g242960 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g242960
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptioncold shock domain-containing protein 3-like
LocationCsor_Chr16: 2482422 .. 2485415 (-)
RNA-Seq ExpressionCsor.00g242960
SyntenyCsor.00g242960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAATTCGAAGCTAAGTGGAATTTTGATTTCGATCTTGGAGATGACGACGACGAGGATTTTCTTTTGGAGGTGGCCGCGGCGGAAGCTCGTGCCCTAGCTAGTAAACGTAGAAAAATCCCCAATCATACCATCGATCCGCCCAAAAGAGCGATCGCGGAGGTTACGAGCAAAGTTTCCCCTAACGCAGATGCGGAAGGGATCTACATCGCCGCTTTGAAAGGAGACCCTATTATCCTCTCGCAACTTTCTGGTCGGATAGCCAATTCTGGAAATAAAGGGTTCATCTCTGCGAATACTACTGCTCATGCTCAATCGGAGGGTGGTGGCTCGTGTTTTAAATGCGGGAAATTAGGGCATTGGGCTAGAGACTGCGATGCTCCGGGAAGTGGAGGGTCTTTCAGCGCTTCAGGAAATGATACGTCTGTGGCCGAGAAATCATGCCCCTGCGGAATGGGAGTATGTGTAGTACTAACTGCGAATACGGAGAGGAATCGAGGTCGAAAATTTTACAAATGTCCCGTTCGACAGGTGAGGAGGCTGCGTTCTTTTTTTGGTATCCTTTCTTCCTCTTCCCCAATGGCCGCCATTGTTGAAGTCATGTAATGATGCCGCGCAAACTTGAGAAATGGAACAGCGATCTTAAGTTCTGATGAATTTATGTCCTTATTCATCGGATTGATGCTGGGTTTTGTCAAAAATATATATGCAGCTATTAAGAACCTTGAGGAAGATATCGGTGTTACTCAAACCTATGTAGCATTCATGTAGAAATTAAATTAGTAATGCGTAATGTAATATTGCCACTGATTCATATAATTTTTCTTGACTAAAACAGTGTTACTAAACATAAATTTTGCTATACCATTGTTAGCTCAAAGCTCATTAGATACTTTTTCTTTACGCAATAACTTTTTATCATCACATGGTTATATATCATGCTCCTACTATCTTTTGATATGCATTTAATTGCTCTCAGGAAAATGGTGGCTGCGGATTCTTTGAGTGGTGTGACAATGCCTCTGCAACTAATATAATGACTTATGGAAGCCAAAATCGTGCATCAAGTTCTTCATTTTCGGACCTTAAGTGCCCCTGTGGTGCTGGTTCTTGCATAACTCTAACAGCCAAAACAGGGGAAAATGTGGGGCAGCAATTCTATCGTTGCCCTTCATACCAGGTAATTTTATAGCAGAAATGTTTATGCAGCAGTTTTTATTGTCTTGTTTAGATTTCGGGTTGCTGAATTTTTATTTTCATATTAAGAAGAATGTCATGAACTATCCAATTATCTGATTGAACTGTGGCAAATCTTGAAATGTATACGCATTTGGTGCTTCATTCAGACTGATGGGAAGAAAGTAAAATTTGCTCTAATACCTTGGCTAAGTATGCTTTTCCTACATTTGTCTCATTTTTTCAAATTTACTTTATGGTCCCAATTTTATGACTCTTCATCTTTGATCAATTGATTCCGCTGGGAGTTGAAAATTATGGTCTTGAAGGTGGCCTAGATTATTGCAAGCAGTTGAGCTTATCGCCAACTACTCTGCATATTTCTCGTTGGCTTAAATGACTAAAGTGTATAAAGACGGATGTTATAGTTACATTATAATGAACTAAGGAAAGAAAGCAACCTTAAGGGGAAGGAACCATTGCGGACGAATGTAATAAGAATATGCTTAATACAAAAAAATTTAGCTTCATTATACCTTTATGTCAGGCTCATATATACCAATTTAAGCACAATCAAGAGAATATGAATGCCTACTCCAGGATGGGTAAGAAGGGCAGTAACCACCAGACAGTTAGTGGGAGATTATGTACGGTTGTTGGGCATGAGATTGAGCTAGGCCTTTAAGTGTCAGGTTGTGTTTTAGGCAGGGGCATGGAATCTTTTGTAATAGGGCAGCCTTCTAAGGTTTTGAAGAGTTTGCTACCCCATTGAATTGGGCGGTTTATGTTCTTCTTGTTTCTCAATGGTGTTAACTTGAATTCAATCAAATAGCGAGGGAATACCCTTATGGAGAATAAGTGAATTGCCGTTTTTAGTGTTCTCCTCGCCTTTTAGATGGTGGGGTTTGTTTCATCTGTATATCACTTGGTCATGACATCCACCCAAGGTTTGCTGTAACTTGTCATGAAGAATAGGTTCATGAATCTTTTAGAATCATGAACAATTGTGCTACATTCCCCTCTCCTATATATTGGAATTTATTTGGCTCATCTCCCATTCATTTGGTTCATCTCCAATTTTTGATACACGAATCATCTTGATTCATGTATGACACTAATGCTTTTATGCCTATATAAAAGGTTGTCTAAGATGGTTGAGAGACATTGAGTTCGATTGAGTTTGAGTGTCATTTTGGGAGGAACTTCTGTAAACACTTTGATTATTGAGATTGGTTGTTGTCCATGGATGTAGAGGAATGTCTTCTCTCCAAAACCACGTAAATCTTTGTGTCTCTTTGTTTAGTTTCTGTTTTGTGGATATCTGAGTGCAATCGATCTCGCTTCCGCTTCCGATACAACACCTCTATCTCTTTAAATTTTCTAGTTCTGTGGTAATGGTAATTTCCAGCACCAACATCATCTGAACATAGTTCTCTGGAAATGCTTCGATGCTTGCAAATTGCACAAGTTGTACAGTATATATATATGAATTGATCTTCCTGTTGTAATTCCTCATAAGCGGTATTCAAATCTGTTCTGCAGGCAACTTGCGGATTTTTCCAGTGGTGCAAGGAGGCATCCATGGCAGCCAAGAATCAAGTGAGTGGCTCTAAGGTCTACGGCAACACAACCGACAGAAGCAAAGGCATGCCTGCGACTGCAAGGGCGGGTTCTTCCTGTTATAAATGTGGGGGCGAAGGGCATTGGGCTAGAGATTGCTCGCAATCACTCGCACCATCAAACCAATCTTCTTCAGCTGGAAGCTGTTTCAAATGTGGGAAGCCTGGCCATTGGGCGAGGGACTGTTCTAATTGTTAA

mRNA sequence

ATGAAATTCGAAGCTAAGTGGAATTTTGATTTCGATCTTGGAGATGACGACGACGAGGATTTTCTTTTGGAGGTGGCCGCGGCGGAAGCTCGTGCCCTAGCTAGTAAACGTAGAAAAATCCCCAATCATACCATCGATCCGCCCAAAAGAGCGATCGCGGAGGTTACGAGCAAAGTTTCCCCTAACGCAGATGCGGAAGGGATCTACATCGCCGCTTTGAAAGGAGACCCTATTATCCTCTCGCAACTTTCTGGTCGGATAGCCAATTCTGGAAATAAAGGGTTCATCTCTGCGAATACTACTGCTCATGCTCAATCGGAGGGTGGTGGCTCGTGTTTTAAATGCGGGAAATTAGGGCATTGGGCTAGAGACTGCGATGCTCCGGGAAGTGGAGGGTCTTTCAGCGCTTCAGGAAATGATACGTCTGTGGCCGAGAAATCATGCCCCTGCGGAATGGGAGTATGTGTAGTACTAACTGCGAATACGGAGAGGAATCGAGGTCGAAAATTTTACAAATGTCCCGTTCGACAGGAAAATGGTGGCTGCGGATTCTTTGAGTGGTGTGACAATGCCTCTGCAACTAATATAATGACTTATGGAAGCCAAAATCGTGCATCAAGTTCTTCATTTTCGGACCTTAAGTGCCCCTGTGGTGCTGGTTCTTGCATAACTCTAACAGCCAAAACAGGGGAAAATGTGGGGCAGCAATTCTATCGTTGCCCTTCATACCAGGCAACTTGCGGATTTTTCCAGTGGTGCAAGGAGGCATCCATGGCAGCCAAGAATCAAGTGAGTGGCTCTAAGGTCTACGGCAACACAACCGACAGAAGCAAAGGCATGCCTGCGACTGCAAGGGCGGGTTCTTCCTGTTATAAATGTGGGGGCGAAGGGCATTGGGCTAGAGATTGCTCGCAATCACTCGCACCATCAAACCAATCTTCTTCAGCTGGAAGCTGTTTCAAATGTGGGAAGCCTGGCCATTGGGCGAGGGACTGTTCTAATTGTTAA

Coding sequence (CDS)

ATGAAATTCGAAGCTAAGTGGAATTTTGATTTCGATCTTGGAGATGACGACGACGAGGATTTTCTTTTGGAGGTGGCCGCGGCGGAAGCTCGTGCCCTAGCTAGTAAACGTAGAAAAATCCCCAATCATACCATCGATCCGCCCAAAAGAGCGATCGCGGAGGTTACGAGCAAAGTTTCCCCTAACGCAGATGCGGAAGGGATCTACATCGCCGCTTTGAAAGGAGACCCTATTATCCTCTCGCAACTTTCTGGTCGGATAGCCAATTCTGGAAATAAAGGGTTCATCTCTGCGAATACTACTGCTCATGCTCAATCGGAGGGTGGTGGCTCGTGTTTTAAATGCGGGAAATTAGGGCATTGGGCTAGAGACTGCGATGCTCCGGGAAGTGGAGGGTCTTTCAGCGCTTCAGGAAATGATACGTCTGTGGCCGAGAAATCATGCCCCTGCGGAATGGGAGTATGTGTAGTACTAACTGCGAATACGGAGAGGAATCGAGGTCGAAAATTTTACAAATGTCCCGTTCGACAGGAAAATGGTGGCTGCGGATTCTTTGAGTGGTGTGACAATGCCTCTGCAACTAATATAATGACTTATGGAAGCCAAAATCGTGCATCAAGTTCTTCATTTTCGGACCTTAAGTGCCCCTGTGGTGCTGGTTCTTGCATAACTCTAACAGCCAAAACAGGGGAAAATGTGGGGCAGCAATTCTATCGTTGCCCTTCATACCAGGCAACTTGCGGATTTTTCCAGTGGTGCAAGGAGGCATCCATGGCAGCCAAGAATCAAGTGAGTGGCTCTAAGGTCTACGGCAACACAACCGACAGAAGCAAAGGCATGCCTGCGACTGCAAGGGCGGGTTCTTCCTGTTATAAATGTGGGGGCGAAGGGCATTGGGCTAGAGATTGCTCGCAATCACTCGCACCATCAAACCAATCTTCTTCAGCTGGAAGCTGTTTCAAATGTGGGAAGCCTGGCCATTGGGCGAGGGACTGTTCTAATTGTTAA

Protein sequence

MKFEAKWNFDFDLGDDDDEDFLLEVAAAEARALASKRRKIPNHTIDPPKRAIAEVTSKVSPNADAEGIYIAALKGDPIILSQLSGRIANSGNKGFISANTTAHAQSEGGGSCFKCGKLGHWARDCDAPGSGGSFSASGNDTSVAEKSCPCGMGVCVVLTANTERNRGRKFYKCPVRQENGGCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYRCPSYQATCGFFQWCKEASMAAKNQVSGSKVYGNTTDRSKGMPATARAGSSCYKCGGEGHWARDCSQSLAPSNQSSSAGSCFKCGKPGHWARDCSNC
Homology
BLAST of Csor.00g242960 vs. ExPASy Swiss-Prot
Match: Q94C69 (Cold shock domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 GN=CSP3 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 1.0e-09
Identity = 72/258 (27.91%), Postives = 91/258 (35.27%), Query Frame = 0

Query: 97  SANTTAHAQSEGGGSCFKCGKLGHWARDCDAPGSGGSFSASGNDTSVAEKSC-PCGMGVC 156
           S N   ++    GG+CF CG++GH A+DCD    G SF   G   S  E  C  CG    
Sbjct: 81  SLNKKENSSRGSGGNCFNCGEVGHMAKDCDGGSGGKSFGGGGGRRSGGEGECYMCG---- 140

Query: 157 VVLTANTERNRGRKFYKCPVRQENGG--------------CG----FFEWCDNASATNIM 216
                    + G     C  RQ  GG              CG      + C   S  N  
Sbjct: 141 ---------DVGHFARDC--RQSGGGNSGGGGGGGRPCYSCGEVGHLAKDCRGGSGGN-- 200

Query: 217 TYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYRCPSYQATCGFFQWCKEAS 276
            YG      S       C              G NVG     C     TCG         
Sbjct: 201 RYGGGGGRGSGGDGCYMCGGVGHFARDCRQNGGGNVGGGGSTC----YTCG--------G 260

Query: 277 MAAKNQVSGSKVYGNTTDRSKGMPATARAGSSCYKCGGEGHWARDCS-QSLAPSNQSSSA 335
           +    +V  SK+                 G +CY+CGG GH ARDC  +    S     +
Sbjct: 261 VGHIAKVCTSKI----------PSGGGGGGRACYECGGTGHLARDCDRRGSGSSGGGGGS 299

BLAST of Csor.00g242960 vs. ExPASy Swiss-Prot
Match: O65639 (Cold shock protein 1 OS=Arabidopsis thaliana OX=3702 GN=CSP1 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.9e-08
Identity = 63/240 (26.25%), Postives = 90/240 (37.50%), Query Frame = 0

Query: 105 QSEGGGSCFKCGKLGHWARDCDAPGSGGSFSAS--GNDTSVAEKSCPCGMGVCVVLTANT 164
           +S GG  C+ CG  GH+ARDC + G+G    A+  GND         CG  V  V    T
Sbjct: 127 RSRGGEGCYNCGDTGHFARDCTSAGNGDQRGATKGGNDGCYT-----CG-DVGHVARDCT 186

Query: 165 ERNRGRKFYKCPVRQENGGC------GFF--EWCDNASATNIMTYGSQNRASSSSFSDLK 224
           +++ G    +  V+  N GC      G F  +     +A N+ + G              
Sbjct: 187 QKSVGNGDQRGAVKGGNDGCYTCGDVGHFARDCTQKVAAGNVRSGGG------------- 246

Query: 225 CPCGAGSCITLTAKTGENVGQQFYRCPSYQATCGFFQWCKEASMAAKNQVSGSKVYGNTT 284
              G+G+C               Y C                              G   
Sbjct: 247 ---GSGTC---------------YSC------------------------------GGVG 297

Query: 285 DRSKGMPATARAGSSCYKCGGEGHWARDCSQSLAPSNQSSSAGSCFKCGKPGHWARDCSN 335
             ++      +    CY+CGG GH ARDC Q    S    +  +C+KCGK GH+AR+CS+
Sbjct: 307 HIARDCATKRQPSRGCYQCGGSGHLARDCDQ--RGSGGGGNDNACYKCGKEGHFARECSS 297

BLAST of Csor.00g242960 vs. ExPASy Swiss-Prot
Match: Q04832 (DNA-binding protein HEXBP OS=Leishmania major OX=5664 GN=HEXBP PE=4 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 5.6e-08
Identity = 70/260 (26.92%), Postives = 97/260 (37.31%), Query Frame = 0

Query: 105 QSEGGGSCFKCGKLGHWARDCDAPGSGGSFSASGNDTSVAEKSCPCGMGVCVVLTANTER 164
           ++E   SC  CGK GH+AR+C    S G            E+S  C            E 
Sbjct: 11  RTESSTSCRNCGKEGHYARECPEADSKGD-----------ERSTTC-------FRCGEEG 70

Query: 165 NRGRKFYKCPVRQENGGCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKC-PCG----- 224
           +  R   +CP    +G  G    C        M+    N A   +    +C  CG     
Sbjct: 71  HMSR---ECPNEARSGAAGAMT-CFRCGEAGHMSRDCPNSAKPGAAKGFECYKCGQEGHL 130

Query: 225 --------AGSCITLTAKTGENVGQQFYRCPSYQATCGFFQWCKEASMAAKNQVSGSKVY 284
                    GS      K G +  Q  Y   S   TC     C +A   +++  +G   Y
Sbjct: 131 SRDCPSSQGGSRGGYGQKRGRSGAQGGY---SGDRTC---YKCGDAGHISRDCPNGQGGY 190

Query: 285 GNTTDRS---------------KGMPATARAGS-SCYKCGGEGHWARDCSQSLAPSNQSS 333
               DR+                G    + AG   CYKCG  GH +R+C     PS  S+
Sbjct: 191 SGAGDRTCYKCGDAGHISRDCPNGQGGYSGAGDRKCYKCGESGHMSREC-----PSAGST 237

BLAST of Csor.00g242960 vs. ExPASy Swiss-Prot
Match: Q9NG98 (DNA topoisomerase 3-alpha OS=Drosophila melanogaster OX=7227 GN=Top3alpha PE=2 SV=2)

HSP 1 Score: 56.2 bits (134), Expect = 8.1e-07
Identity = 47/193 (24.35%), Postives = 66/193 (34.20%), Query Frame = 0

Query: 165  NRGRKFYKCPVRQENGGCGFFEWCD----------------------------------- 224
            N GR +YKCP   E   C FF+W D                                   
Sbjct: 1053 NLGRLYYKCPKPDE---CNFFQWADEPPSSAKSKNSTGSAPQSTTSWGSNRVVTLPSIQQ 1112

Query: 225  ------------NASATNIMT-----YGSQNRASSSSFSDLKCPCGAGSCITLTAKTGEN 284
                        N+S+T  +T        +N A+     ++ C CG  +      K G N
Sbjct: 1113 SNSQRGQSSMRSNSSSTVTITQTKTKQQERNTATPGDGEEVMCNCGQLASQLTVRKDGPN 1172

Query: 285  VGQQFYRCPSYQATCGFFQWCKEASMAAKNQVSGSKVYGNTTDRSKGMPATARAGSS--C 304
             G+ FY CP+ + +CGFF+W  E      +  S      N   RS+    T+    +  C
Sbjct: 1173 QGRPFYACPTREKSCGFFKWGDEDQNQGASSTSWGSANRNPPGRSQPTAITSDGPKTRRC 1232

BLAST of Csor.00g242960 vs. ExPASy Swiss-Prot
Match: P27484 (Glycine-rich protein 2 OS=Nicotiana sylvestris OX=4096 GN=GRP-2 PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 1.4e-06
Identity = 25/56 (44.64%), Postives = 33/56 (58.93%), Query Frame = 0

Query: 287 GSSCYKCGGEGHWARDCSQS--------LAPSNQSSSAGSCFKCGKPGHWARDCSN 335
           GS C+KCG  GH+ARDCSQS                  G C+KCG+ GH+AR+C++
Sbjct: 156 GSGCFKCGESGHFARDCSQSGGGGGGGRFGGGGGGGGGGGCYKCGEDGHFARECTS 211

BLAST of Csor.00g242960 vs. NCBI nr
Match: KAG6577083.1 (DNA topoisomerase 3-alpha, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 693 bits (1788), Expect = 6.01e-252
Identity = 335/335 (100.00%), Postives = 335/335 (100.00%), Query Frame = 0

Query: 1   MKFEAKWNFDFDLGDDDDEDFLLEVAAAEARALASKRRKIPNHTIDPPKRAIAEVTSKVS 60
           MKFEAKWNFDFDLGDDDDEDFLLEVAAAEARALASKRRKIPNHTIDPPKRAIAEVTSKVS
Sbjct: 1   MKFEAKWNFDFDLGDDDDEDFLLEVAAAEARALASKRRKIPNHTIDPPKRAIAEVTSKVS 60

Query: 61  PNADAEGIYIAALKGDPIILSQLSGRIANSGNKGFISANTTAHAQSEGGGSCFKCGKLGH 120
           PNADAEGIYIAALKGDPIILSQLSGRIANSGNKGFISANTTAHAQSEGGGSCFKCGKLGH
Sbjct: 61  PNADAEGIYIAALKGDPIILSQLSGRIANSGNKGFISANTTAHAQSEGGGSCFKCGKLGH 120

Query: 121 WARDCDAPGSGGSFSASGNDTSVAEKSCPCGMGVCVVLTANTERNRGRKFYKCPVRQENG 180
           WARDCDAPGSGGSFSASGNDTSVAEKSCPCGMGVCVVLTANTERNRGRKFYKCPVRQENG
Sbjct: 121 WARDCDAPGSGGSFSASGNDTSVAEKSCPCGMGVCVVLTANTERNRGRKFYKCPVRQENG 180

Query: 181 GCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYRC 240
           GCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYRC
Sbjct: 181 GCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYRC 240

Query: 241 PSYQATCGFFQWCKEASMAAKNQVSGSKVYGNTTDRSKGMPATARAGSSCYKCGGEGHWA 300
           PSYQATCGFFQWCKEASMAAKNQVSGSKVYGNTTDRSKGMPATARAGSSCYKCGGEGHWA
Sbjct: 241 PSYQATCGFFQWCKEASMAAKNQVSGSKVYGNTTDRSKGMPATARAGSSCYKCGGEGHWA 300

Query: 301 RDCSQSLAPSNQSSSAGSCFKCGKPGHWARDCSNC 335
           RDCSQSLAPSNQSSSAGSCFKCGKPGHWARDCSNC
Sbjct: 301 RDCSQSLAPSNQSSSAGSCFKCGKPGHWARDCSNC 335

BLAST of Csor.00g242960 vs. NCBI nr
Match: KAG7015092.1 (DNA topoisomerase 3-alpha, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 682 bits (1760), Expect = 1.20e-247
Identity = 332/334 (99.40%), Postives = 332/334 (99.40%), Query Frame = 0

Query: 1   MKFEAKWNFDFDLGDDDDEDFLLEVAAAEARALASKRRKIPNHTIDPPKRAIAEVTSKVS 60
           MKFEAK NFDFDLGDDDDEDFL EVAAAEARALASKRRKIPNHTIDPPKRAIAEVTSKVS
Sbjct: 1   MKFEAKRNFDFDLGDDDDEDFLSEVAAAEARALASKRRKIPNHTIDPPKRAIAEVTSKVS 60

Query: 61  PNADAEGIYIAALKGDPIILSQLSGRIANSGNKGFISANTTAHAQSEGGGSCFKCGKLGH 120
           PNADAEGIYIAALKGDPIILSQLSGRIANSGNKGFISANTTAHAQSEGGGSCFKCGKLGH
Sbjct: 61  PNADAEGIYIAALKGDPIILSQLSGRIANSGNKGFISANTTAHAQSEGGGSCFKCGKLGH 120

Query: 121 WARDCDAPGSGGSFSASGNDTSVAEKSCPCGMGVCVVLTANTERNRGRKFYKCPVRQENG 180
           WARDCDAPGSGGSFSASGNDTSVAEKSCPCGMGVCVVLTANTERNRGRKFYKCPVRQENG
Sbjct: 121 WARDCDAPGSGGSFSASGNDTSVAEKSCPCGMGVCVVLTANTERNRGRKFYKCPVRQENG 180

Query: 181 GCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYRC 240
           GCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYRC
Sbjct: 181 GCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYRC 240

Query: 241 PSYQATCGFFQWCKEASMAAKNQVSGSKVYGNTTDRSKGMPATARAGSSCYKCGGEGHWA 300
           PSYQATCGFFQWCKEASMAAKNQVSGSKVYGNTTDRSKGMPATARAGSSCYKCGGEGHWA
Sbjct: 241 PSYQATCGFFQWCKEASMAAKNQVSGSKVYGNTTDRSKGMPATARAGSSCYKCGGEGHWA 300

Query: 301 RDCSQSLAPSNQSSSAGSCFKCGKPGHWARDCSN 334
           RDCSQSLAPSNQSSSAGSCFKCGKPGHWARDCSN
Sbjct: 301 RDCSQSLAPSNQSSSAGSCFKCGKPGHWARDCSN 334

BLAST of Csor.00g242960 vs. NCBI nr
Match: XP_022931285.1 (cold shock domain-containing protein 3-like [Cucurbita moschata])

HSP 1 Score: 676 bits (1744), Expect = 3.19e-245
Identity = 331/336 (98.51%), Postives = 331/336 (98.51%), Query Frame = 0

Query: 1   MKFEAKWNFDFDLGDDDD-EDFLLEVAAAEARALASKRRKIPNHTIDPPKRAIAEVTSKV 60
           MKFEAK NFDFDLGDDDD EDFL EVAAAEARALASKRRKIPNHTIDPPKRAIAEVTSKV
Sbjct: 1   MKFEAKRNFDFDLGDDDDDEDFLSEVAAAEARALASKRRKIPNHTIDPPKRAIAEVTSKV 60

Query: 61  SPNADAEGIYIAALKGDPIILSQLSGRIANSGNKGFISANTTAHAQSEGGGSCFKCGKLG 120
           SPNADAEGIYIAALKGDPIILSQLSGRIANSGNKGFISANTTAHAQSEGGGSCFKCGKLG
Sbjct: 61  SPNADAEGIYIAALKGDPIILSQLSGRIANSGNKGFISANTTAHAQSEGGGSCFKCGKLG 120

Query: 121 HWARDCDAPGSGGSFSASGNDTSVAEKSCPCGMGVCVVLTANTERNRGRKFYKCPVRQEN 180
           HWARDCDAPGSGGSFS SGNDTSVAEKSCPCGMGVCVVLTANTERNRGRKFYKCPVRQEN
Sbjct: 121 HWARDCDAPGSGGSFSFSGNDTSVAEKSCPCGMGVCVVLTANTERNRGRKFYKCPVRQEN 180

Query: 181 GGCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYR 240
           GGCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYR
Sbjct: 181 GGCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYR 240

Query: 241 CPSYQATCGFFQWCKEASMAAKNQVSGSKVYGNTTDRSKGMPATARAGSSCYKCGGEGHW 300
           CPSYQATCGFFQWCKEASMAAKNQVSGSKVYGNTTD SKGMPATARAGSSCYKCGGEGHW
Sbjct: 241 CPSYQATCGFFQWCKEASMAAKNQVSGSKVYGNTTDTSKGMPATARAGSSCYKCGGEGHW 300

Query: 301 ARDCSQSLAPSNQSSSAGSCFKCGKPGHWARDCSNC 335
           ARDCSQSLAPSNQSSSAGSCFKCGKPGHWARDCSNC
Sbjct: 301 ARDCSQSLAPSNQSSSAGSCFKCGKPGHWARDCSNC 336

BLAST of Csor.00g242960 vs. NCBI nr
Match: XP_023552737.1 (cold shock domain-containing protein 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 666 bits (1718), Expect = 3.95e-241
Identity = 326/344 (94.77%), Postives = 329/344 (95.64%), Query Frame = 0

Query: 1   MKFEAKWNFDFDLGDDDDEDFLLEVAAAEARALASKRRKIPNHTIDPPKRAIAEVTSKVS 60
           MKFEAK NFDFDLGDDDDEDFL EVAAAEARALASKRRKIPNHTIDPPKRA+AEVTSKVS
Sbjct: 1   MKFEAKRNFDFDLGDDDDEDFLSEVAAAEARALASKRRKIPNHTIDPPKRAVAEVTSKVS 60

Query: 61  PNADAEGIYIAALKGDPIILSQLSGRIANSGNKGFISANTTAHAQSEGGGSCFKCGKLGH 120
           PNADAEGIYIAALKGDPIILSQLSGRIANSGNKGFISANTTAHAQSEGGGSCFKCGKLGH
Sbjct: 61  PNADAEGIYIAALKGDPIILSQLSGRIANSGNKGFISANTTAHAQSEGGGSCFKCGKLGH 120

Query: 121 WARDCDAPGSGGSFSASGNDTSVAEKSCPCGMGVCVVLTANTERNRGRKFYKCPVRQENG 180
           WARDCDAPGSGGSFSASGNDTSVAEKSCPCGMGVCVVLTANTERNRGRKFYKC VRQENG
Sbjct: 121 WARDCDAPGSGGSFSASGNDTSVAEKSCPCGMGVCVVLTANTERNRGRKFYKCAVRQENG 180

Query: 181 GCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYRC 240
           GCGFFEWCDNAS+TNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTG+NVGQQFYRC
Sbjct: 181 GCGFFEWCDNASSTNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGDNVGQQFYRC 240

Query: 241 PSYQATCGFFQWCKEASMAAKNQVSGSKVYGNTTDRSKGMPATARAGSSCYKCGGEGHWA 300
           PSYQATCGFFQWCKE SMAAKNQ SGSKVYGNTTD SKGMPATARAGSSCYKCGGEGHWA
Sbjct: 241 PSYQATCGFFQWCKEPSMAAKNQASGSKVYGNTTDTSKGMPATARAGSSCYKCGGEGHWA 300

Query: 301 RDCSQSLAPSN---------QSSSAGSCFKCGKPGHWARDCSNC 335
           RDCSQSLAPSN         QSSSAGSCFKCGKPGHWARDCSNC
Sbjct: 301 RDCSQSLAPSNPPPAEFGRNQSSSAGSCFKCGKPGHWARDCSNC 344

BLAST of Csor.00g242960 vs. NCBI nr
Match: XP_022984959.1 (cold shock domain-containing protein 3-like [Cucurbita maxima])

HSP 1 Score: 655 bits (1690), Expect = 7.61e-237
Identity = 324/345 (93.91%), Postives = 327/345 (94.78%), Query Frame = 0

Query: 1   MKFEAKWNFDFDLGDDDD-EDFLLEVAAAEARALASKRRKIPNHTIDPPKRAIAEVTSKV 60
           MKFEAK NFDFDLGDDDD EDFL EVAAAEARALASKRRKIPNHTI+PPKRAIAEVT+KV
Sbjct: 1   MKFEAKRNFDFDLGDDDDDEDFLSEVAAAEARALASKRRKIPNHTINPPKRAIAEVTNKV 60

Query: 61  SPNADAEGIYIAALKGDPIILSQLSGRIANSGNKGFISANTTAHAQSEGGGSCFKCGKLG 120
           SPN DAEGIYIAALKGDPIILSQLSGRIANSGNKGFISANTTAHAQS+GGGSCFKCGKLG
Sbjct: 61  SPNVDAEGIYIAALKGDPIILSQLSGRIANSGNKGFISANTTAHAQSDGGGSCFKCGKLG 120

Query: 121 HWARDCDAPGSGGSFSASGNDTSVAEKSCPCGMGVCVVLTANTERNRGRKFYKCPVRQEN 180
           HWARDCDAPGSGGSFSAS NDTSVAEKSCPCG GVCVVLTANTERNRGRKFYKCPVRQEN
Sbjct: 121 HWARDCDAPGSGGSFSASENDTSVAEKSCPCGRGVCVVLTANTERNRGRKFYKCPVRQEN 180

Query: 181 GGCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYR 240
           GGCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYR
Sbjct: 181 GGCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYR 240

Query: 241 CPSYQATCGFFQWCKEASMAAKNQVSGSKVYGNTTDRSKGMPATARAGSSCYKCGGEGHW 300
           CPSYQATCGFFQWCKE SMAAKNQVS SKVYGNTTD SKGMPATARAGSSCYKCGGEGHW
Sbjct: 241 CPSYQATCGFFQWCKEPSMAAKNQVSASKVYGNTTDTSKGMPATARAGSSCYKCGGEGHW 300

Query: 301 ARDCSQSLAPSN---------QSSSAGSCFKCGKPGHWARDCSNC 335
           ARDCSQSLAPSN         QSSSAGSCFKCGKPGHWARDCSNC
Sbjct: 301 ARDCSQSLAPSNPPPAEFGRNQSSSAGSCFKCGKPGHWARDCSNC 345

BLAST of Csor.00g242960 vs. ExPASy TrEMBL
Match: A0A6J1EZ11 (cold shock domain-containing protein 3-like OS=Cucurbita moschata OX=3662 GN=LOC111437513 PE=4 SV=1)

HSP 1 Score: 676 bits (1744), Expect = 1.54e-245
Identity = 331/336 (98.51%), Postives = 331/336 (98.51%), Query Frame = 0

Query: 1   MKFEAKWNFDFDLGDDDD-EDFLLEVAAAEARALASKRRKIPNHTIDPPKRAIAEVTSKV 60
           MKFEAK NFDFDLGDDDD EDFL EVAAAEARALASKRRKIPNHTIDPPKRAIAEVTSKV
Sbjct: 1   MKFEAKRNFDFDLGDDDDDEDFLSEVAAAEARALASKRRKIPNHTIDPPKRAIAEVTSKV 60

Query: 61  SPNADAEGIYIAALKGDPIILSQLSGRIANSGNKGFISANTTAHAQSEGGGSCFKCGKLG 120
           SPNADAEGIYIAALKGDPIILSQLSGRIANSGNKGFISANTTAHAQSEGGGSCFKCGKLG
Sbjct: 61  SPNADAEGIYIAALKGDPIILSQLSGRIANSGNKGFISANTTAHAQSEGGGSCFKCGKLG 120

Query: 121 HWARDCDAPGSGGSFSASGNDTSVAEKSCPCGMGVCVVLTANTERNRGRKFYKCPVRQEN 180
           HWARDCDAPGSGGSFS SGNDTSVAEKSCPCGMGVCVVLTANTERNRGRKFYKCPVRQEN
Sbjct: 121 HWARDCDAPGSGGSFSFSGNDTSVAEKSCPCGMGVCVVLTANTERNRGRKFYKCPVRQEN 180

Query: 181 GGCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYR 240
           GGCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYR
Sbjct: 181 GGCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYR 240

Query: 241 CPSYQATCGFFQWCKEASMAAKNQVSGSKVYGNTTDRSKGMPATARAGSSCYKCGGEGHW 300
           CPSYQATCGFFQWCKEASMAAKNQVSGSKVYGNTTD SKGMPATARAGSSCYKCGGEGHW
Sbjct: 241 CPSYQATCGFFQWCKEASMAAKNQVSGSKVYGNTTDTSKGMPATARAGSSCYKCGGEGHW 300

Query: 301 ARDCSQSLAPSNQSSSAGSCFKCGKPGHWARDCSNC 335
           ARDCSQSLAPSNQSSSAGSCFKCGKPGHWARDCSNC
Sbjct: 301 ARDCSQSLAPSNQSSSAGSCFKCGKPGHWARDCSNC 336

BLAST of Csor.00g242960 vs. ExPASy TrEMBL
Match: A0A6J1JBZ0 (cold shock domain-containing protein 3-like OS=Cucurbita maxima OX=3661 GN=LOC111483073 PE=4 SV=1)

HSP 1 Score: 655 bits (1690), Expect = 3.68e-237
Identity = 324/345 (93.91%), Postives = 327/345 (94.78%), Query Frame = 0

Query: 1   MKFEAKWNFDFDLGDDDD-EDFLLEVAAAEARALASKRRKIPNHTIDPPKRAIAEVTSKV 60
           MKFEAK NFDFDLGDDDD EDFL EVAAAEARALASKRRKIPNHTI+PPKRAIAEVT+KV
Sbjct: 1   MKFEAKRNFDFDLGDDDDDEDFLSEVAAAEARALASKRRKIPNHTINPPKRAIAEVTNKV 60

Query: 61  SPNADAEGIYIAALKGDPIILSQLSGRIANSGNKGFISANTTAHAQSEGGGSCFKCGKLG 120
           SPN DAEGIYIAALKGDPIILSQLSGRIANSGNKGFISANTTAHAQS+GGGSCFKCGKLG
Sbjct: 61  SPNVDAEGIYIAALKGDPIILSQLSGRIANSGNKGFISANTTAHAQSDGGGSCFKCGKLG 120

Query: 121 HWARDCDAPGSGGSFSASGNDTSVAEKSCPCGMGVCVVLTANTERNRGRKFYKCPVRQEN 180
           HWARDCDAPGSGGSFSAS NDTSVAEKSCPCG GVCVVLTANTERNRGRKFYKCPVRQEN
Sbjct: 121 HWARDCDAPGSGGSFSASENDTSVAEKSCPCGRGVCVVLTANTERNRGRKFYKCPVRQEN 180

Query: 181 GGCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYR 240
           GGCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYR
Sbjct: 181 GGCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYR 240

Query: 241 CPSYQATCGFFQWCKEASMAAKNQVSGSKVYGNTTDRSKGMPATARAGSSCYKCGGEGHW 300
           CPSYQATCGFFQWCKE SMAAKNQVS SKVYGNTTD SKGMPATARAGSSCYKCGGEGHW
Sbjct: 241 CPSYQATCGFFQWCKEPSMAAKNQVSASKVYGNTTDTSKGMPATARAGSSCYKCGGEGHW 300

Query: 301 ARDCSQSLAPSN---------QSSSAGSCFKCGKPGHWARDCSNC 335
           ARDCSQSLAPSN         QSSSAGSCFKCGKPGHWARDCSNC
Sbjct: 301 ARDCSQSLAPSNPPPAEFGRNQSSSAGSCFKCGKPGHWARDCSNC 345

BLAST of Csor.00g242960 vs. ExPASy TrEMBL
Match: A0A6J1C4N6 (DNA-binding protein HEXBP-like OS=Momordica charantia OX=3673 GN=LOC111008238 PE=4 SV=1)

HSP 1 Score: 512 bits (1318), Expect = 2.28e-180
Identity = 257/364 (70.60%), Postives = 279/364 (76.65%), Query Frame = 0

Query: 1   MKFEAKWNFDFDLGDDDDEDFLLEVAAAEARALASKRRKIPNHTIDPPKRAIAEVTSKVS 60
           MK EAK NF+FDL DDDDEDFL  VAAAEARALASKRR+IPNHTIDPPK AIAE      
Sbjct: 1   MKIEAKRNFEFDLEDDDDEDFLSAVAAAEARALASKRRRIPNHTIDPPKMAIAE------ 60

Query: 61  PNADAEGIYIAALKGDPIILSQLSGRIANS-------GNKGFISANTTAHAQSEGGGSCF 120
              + EG+Y+AAL+G P + SQ+SG  ANS        N+GF+SAN TA  QSEGGGSCF
Sbjct: 61  ---EPEGLYMAALRGKPNLPSQISGGTANSRAKGVAAANRGFVSANPTA--QSEGGGSCF 120

Query: 121 KCGKLGHWARDCDAPGSGGSFSASGNDTSVAEKSCPCGMGVCVVLTANTERNRGRKFYKC 180
           KCGKLGHWARDCDAPG GGSF  +G D SVAEK+CPCG+GVC+VLTANTERNRGRKFYKC
Sbjct: 121 KCGKLGHWARDCDAPGGGGSFGTAGTDASVAEKACPCGLGVCIVLTANTERNRGRKFYKC 180

Query: 181 PVRQENGGCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENV 240
           PVRQENGGCGFFEWCD+AS TN+MTYGSQNRASSS FSDL CPCGAGSCI LTAKTGENV
Sbjct: 181 PVRQENGGCGFFEWCDSASGTNVMTYGSQNRASSSLFSDLMCPCGAGSCIILTAKTGENV 240

Query: 241 GQQFYRCPSYQATCGFFQWCKEASMAAKNQVSGSKVYGNTTDRSKGMPAT---------- 300
           GQQFYRCPSYQ +CGFFQWCKE  MA KNQV+  K+YGN  D SK    T          
Sbjct: 241 GQQFYRCPSYQESCGFFQWCKEPPMATKNQVNDYKIYGNANDTSKSFAPTPRTSSSIDAS 300

Query: 301 ----ARAGSSCYKCGGEGHWARDCSQSLAPSN--------QSSSAGSCFKCGKPGHWARD 335
                R GSSCYKCGGEGHWARDCSQS   SN          S++G+CFKC KPGHWARD
Sbjct: 301 RSFAVRTGSSCYKCGGEGHWARDCSQSHTSSNPPFAELGRNPSASGTCFKCSKPGHWARD 353

BLAST of Csor.00g242960 vs. ExPASy TrEMBL
Match: A0A0A0KVB6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G062410 PE=4 SV=1)

HSP 1 Score: 410 bits (1054), Expect = 1.28e-140
Identity = 214/318 (67.30%), Postives = 237/318 (74.53%), Query Frame = 0

Query: 27  AAEARALASKRRKIPNHTIDPPKRAIAEVTSKVSPNADAEGIYIAALKGDPIILSQLSGR 86
           AAE  +L  K R +PN TID PK A+AEV+ KV  NAD  G YIA  KG    LSQ+SG 
Sbjct: 11  AAEVVSLVKKVRNVPNPTIDLPKNAVAEVSHKVPLNADFLGTYIAPYKGSRA-LSQISGS 70

Query: 87  IANSGNKGFISANTTAHAQSEGGGSCFKCGKLGHWARDCDAPGSGGSFSASGNDTSVAEK 146
            A+S     I  NTTA  QSEG GSCFKCGKLGHWARDCDAPG GGSFS+SGND SV +K
Sbjct: 71  EASSDKNVSIYENTTA--QSEGDGSCFKCGKLGHWARDCDAPGGGGSFSSSGNDMSVPDK 130

Query: 147 SCPCGMGVCVVLTANTERNRGRKFYKCPVRQENGGCGFFEWCDNASATNIMTYGSQNRAS 206
           +CPCG G+C VLTANTERNRGRKFYKCPVRQENGGCGFFEWCD+AS  N+++YGSQN  S
Sbjct: 131 ACPCGSGICSVLTANTERNRGRKFYKCPVRQENGGCGFFEWCDSASVANVVSYGSQNPLS 190

Query: 207 SSSFSDLKCPCGAGSCITLTAKTGENVGQQFYRCPSYQATCGFFQWCKEASMAAKNQVSG 266
           SS FSDL+CPCGAGSC  LTAKTG NVG+QFY CPS QA+C FF+WC+E SM  KNQ S 
Sbjct: 191 SS-FSDLQCPCGAGSCKILTAKTGNNVGKQFYCCPSSQASCRFFRWCEEPSMETKNQEST 250

Query: 267 SKVYGNTTDRSKGMPATAR--AGSSCYKCGGEGHWARDCSQSLAPS--------NQSSSA 326
            KVYGNT D SK      +  + SS YK G EGHWARD SQS + S        +QSSSA
Sbjct: 251 PKVYGNTNDTSKSATLNVKTSSSSSSYKYGSEGHWARDWSQSPSSSTSPAEYGRSQSSSA 310

Query: 327 GSCFKCGKPGHWARDCSN 334
           GSCFKCG PGHWARDCSN
Sbjct: 311 GSCFKCGMPGHWARDCSN 324

BLAST of Csor.00g242960 vs. ExPASy TrEMBL
Match: A0A2P5AI83 (DNA topoisomerase, type IA OS=Parasponia andersonii OX=3476 GN=PanWU01x14_329900 PE=4 SV=1)

HSP 1 Score: 347 bits (891), Expect = 1.13e-115
Identity = 186/347 (53.60%), Postives = 230/347 (66.28%), Query Frame = 0

Query: 1   MKFEAKWNF-DFDLGDDDDEDFLLEVAAAEARALASKRRKIPNHTIDPPKRAIAEVTSKV 60
           MK E K +F + D  + DDEDFL  VAAAEA ALASKRR+I     +    A      +V
Sbjct: 1   MKMERKKSFIEVDEEEYDDEDFLSHVAAAEANALASKRRRITPSAANAIVFASPATAKQV 60

Query: 61  SPNADAEGIYIAALKGDPIILSQLSGRIANSGNKGFISANTTAHAQSEGGGSCFKCGKLG 120
           S +AD +G+Y+AALKG+  +   L    A+   +G +       A + GG SCFKCGK G
Sbjct: 61  SSSAD-DGVYLAALKGNQTL--PLRPTTASDPLRGRVPKGRDDEALAGGGDSCFKCGKSG 120

Query: 121 HWARDCDAPGSGGSFSAS---GNDTSVAEKSCPCGMGVCVVLTANTERNRGRKFYKCPVR 180
           HWARDCD  G GG    +   G D S+ EKSCPCG+G+C VLTANTE+NRGRKFYKCP+R
Sbjct: 121 HWARDCDGGGGGGGHHGNYGGGGDPSIPEKSCPCGLGICTVLTANTEKNRGRKFYKCPLR 180

Query: 181 QENGGCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQ 240
           QENGGCGFFEWCDN S  N M  GS    S+SS+  L+CPCGAG C+ LTAK+G NVGQQ
Sbjct: 181 QENGGCGFFEWCDNVSGANSMAGGSYT--SNSSYPGLQCPCGAGLCMILTAKSGNNVGQQ 240

Query: 241 FYRCPSYQ-ATCGFFQWCKEASMAAKNQVSGSKVYGNTTDRSKGMPATARAGSSCYKCGG 300
           FYRCP+ Q ++CGFF+WC E +++    V+ SKV  N +D +       R GSSC+KCG 
Sbjct: 241 FYRCPANQGSSCGFFKWCNEQTVSVGRPVTSSKVSHNLSDTNNSS-YNVRTGSSCFKCGK 300

Query: 301 EGHWARDCS-------QSLAPSNQSSSAGSCFKCGKPGHWARDCSNC 335
           EGHWARDCS         L   + S+SAG+C+KCGKPGHW+RDC+ C
Sbjct: 301 EGHWARDCSVPSSNPTAELGGRSGSASAGTCYKCGKPGHWSRDCTFC 341

BLAST of Csor.00g242960 vs. TAIR 10
Match: AT3G42860.1 (zinc knuckle (CCHC-type) family protein )

HSP 1 Score: 245.0 bits (624), Expect = 8.7e-65
Identity = 139/322 (43.17%), Postives = 182/322 (56.52%), Query Frame = 0

Query: 16  DDDEDFLLEVAAAEARALASKRRKIPNHTIDPPKRAIAEVTSKVSPNADAEGIYIAALKG 75
           D+++DFLL++AA EA A A+KR ++                     ++  EG Y+AALKG
Sbjct: 12  DEEDDFLLQLAAIEAEA-AAKRPRV---------------------SSIPEGPYMAALKG 71

Query: 76  DPIILSQLSGRIANSGNKGFISANTTAHAQSEGGGSCFKCGKLGHWARDCDAPGSGGSFS 135
                 Q      N  +K    A TT   Q   GG        G  A + D P       
Sbjct: 72  SK--SDQWQQSPLNPASKSRSVAVTTGGFQRSDGG--------GGVAGEQDFP------- 131

Query: 136 ASGNDTSVAEKSCPCGMGVCVVLTANTERNRGRKFYKCPVRQENGGCGFFEWCD--NASA 195
                    EKSCPCG+G+C++LT+NT +N GRKFYKCP R+ENGGCGFF+WCD   +S 
Sbjct: 132 ---------EKSCPCGVGICLILTSNTPKNPGRKFYKCPNREENGGCGFFQWCDAVQSSG 191

Query: 196 TNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYRCPSYQATCGFFQWC 255
           T+  T  S    + + F D +CPCGAG C  LTAKTGENVG+QFYRCP ++ +CGFF+WC
Sbjct: 192 TSTTTSNSYGNGNDTKFPDHQCPCGAGLCRVLTAKTGENVGRQFYRCPVFEGSCGFFKWC 251

Query: 256 KEASMAAKNQVSGSKVYGNTTDRSKGMPATARAGSSCYKCGGEGHWARDCS--QSLAPSN 315
            +  +++    S +K   N  D        A+ G+ CYKCG EGHWARDC+      P  
Sbjct: 252 NDNVVSSPTSYSVTK-NSNFGDSDTRGYQNAKTGTPCYKCGKEGHWARDCTVQSDTGPVK 284

Query: 316 QSSSAGSCFKCGKPGHWARDCS 334
            +S+AG CFKCGKPGHW+RDC+
Sbjct: 312 STSAAGDCFKCGKPGHWSRDCT 284

BLAST of Csor.00g242960 vs. TAIR 10
Match: AT5G13920.1 (GRF zinc finger / Zinc knuckle protein )

HSP 1 Score: 87.4 bits (215), Expect = 2.3e-17
Identity = 51/145 (35.17%), Postives = 65/145 (44.83%), Query Frame = 0

Query: 110 GSCFKCGKLGHWARDCDAPGSGGSFSASGNDTSVAEKSCPCGMGVCVVLTANTERNRGRK 169
           G CF+C + GHW  DC           S  D       CPCG G C +  ANT  N GRK
Sbjct: 9   GDCFRCRQAGHWINDCPL--------KSYTDDPPPAIQCPCGGGFCEIKVANTRENPGRK 68

Query: 170 FYKCPVRQENGGCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKT 229
           FYKCP  Q    C FF+WCD  +  +I            +F+   C CGAG C     + 
Sbjct: 69  FYKCPTAQ---NCCFFKWCDKVTDEDI--------KFRPAFTIPICSCGAGPC----RRV 128

Query: 230 GENVGQQFYRCPSYQ--ATCGFFQW 253
            +  G+ +  C   +    CGFF+W
Sbjct: 129 KDVSGRAYLICCIKKGFGACGFFKW 130

BLAST of Csor.00g242960 vs. TAIR 10
Match: AT2G17870.1 (cold shock domain protein 3 )

HSP 1 Score: 65.9 bits (159), Expect = 7.3e-11
Identity = 72/258 (27.91%), Postives = 91/258 (35.27%), Query Frame = 0

Query: 97  SANTTAHAQSEGGGSCFKCGKLGHWARDCDAPGSGGSFSASGNDTSVAEKSC-PCGMGVC 156
           S N   ++    GG+CF CG++GH A+DCD    G SF   G   S  E  C  CG    
Sbjct: 81  SLNKKENSSRGSGGNCFNCGEVGHMAKDCDGGSGGKSFGGGGGRRSGGEGECYMCG---- 140

Query: 157 VVLTANTERNRGRKFYKCPVRQENGG--------------CG----FFEWCDNASATNIM 216
                    + G     C  RQ  GG              CG      + C   S  N  
Sbjct: 141 ---------DVGHFARDC--RQSGGGNSGGGGGGGRPCYSCGEVGHLAKDCRGGSGGN-- 200

Query: 217 TYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYRCPSYQATCGFFQWCKEAS 276
            YG      S       C              G NVG     C     TCG         
Sbjct: 201 RYGGGGGRGSGGDGCYMCGGVGHFARDCRQNGGGNVGGGGSTC----YTCG--------G 260

Query: 277 MAAKNQVSGSKVYGNTTDRSKGMPATARAGSSCYKCGGEGHWARDCS-QSLAPSNQSSSA 335
           +    +V  SK+                 G +CY+CGG GH ARDC  +    S     +
Sbjct: 261 VGHIAKVCTSKI----------PSGGGGGGRACYECGGTGHLARDCDRRGSGSSGGGGGS 299

BLAST of Csor.00g242960 vs. TAIR 10
Match: AT4G36020.1 (cold shock domain protein 1 )

HSP 1 Score: 61.6 bits (148), Expect = 1.4e-09
Identity = 63/240 (26.25%), Postives = 90/240 (37.50%), Query Frame = 0

Query: 105 QSEGGGSCFKCGKLGHWARDCDAPGSGGSFSAS--GNDTSVAEKSCPCGMGVCVVLTANT 164
           +S GG  C+ CG  GH+ARDC + G+G    A+  GND         CG  V  V    T
Sbjct: 127 RSRGGEGCYNCGDTGHFARDCTSAGNGDQRGATKGGNDGCYT-----CG-DVGHVARDCT 186

Query: 165 ERNRGRKFYKCPVRQENGGC------GFF--EWCDNASATNIMTYGSQNRASSSSFSDLK 224
           +++ G    +  V+  N GC      G F  +     +A N+ + G              
Sbjct: 187 QKSVGNGDQRGAVKGGNDGCYTCGDVGHFARDCTQKVAAGNVRSGGG------------- 246

Query: 225 CPCGAGSCITLTAKTGENVGQQFYRCPSYQATCGFFQWCKEASMAAKNQVSGSKVYGNTT 284
              G+G+C               Y C                              G   
Sbjct: 247 ---GSGTC---------------YSC------------------------------GGVG 297

Query: 285 DRSKGMPATARAGSSCYKCGGEGHWARDCSQSLAPSNQSSSAGSCFKCGKPGHWARDCSN 335
             ++      +    CY+CGG GH ARDC Q    S    +  +C+KCGK GH+AR+CS+
Sbjct: 307 HIARDCATKRQPSRGCYQCGGSGHLARDCDQ--RGSGGGGNDNACYKCGKEGHFARECSS 297

BLAST of Csor.00g242960 vs. TAIR 10
Match: AT3G43590.1 (zinc knuckle (CCHC-type) family protein )

HSP 1 Score: 54.3 bits (129), Expect = 2.2e-07
Identity = 55/240 (22.92%), Postives = 85/240 (35.42%), Query Frame = 0

Query: 108 GGGSCFKCGKLGHWARDCDAPGSGGSFSASGNDTSVAEKSCPCGMGVCVVLTANTERNRG 167
           G  SC+ CG+ GH + +C  P                 K C  G   C +      +  G
Sbjct: 164 GWVSCYSCGEQGHTSFNCPTPTKRRKPCFICGSLEHGAKQCSKGHD-CYIC-----KKTG 223

Query: 168 RKFYKCPVRQENGGCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTA 227
            +   CP + +NG  G    C             +   S     D++C            
Sbjct: 224 HRAKDCPDKYKNGSKGAV--CLRCGDFGHDMILCKYEYSKEDLKDVQC------------ 283

Query: 228 KTGENVGQQFYRCPSYQATC-----GFFQW---CKEASMAAKNQVSGSKVYGNTTDRSKG 287
                     Y C S+   C         W   C        + ++  + Y  + +    
Sbjct: 284 ----------YICKSFGHLCCVEPGNSLSWAVSCYRCGQLGHSGLACGRHYEESNENDSA 343

Query: 288 MPA---TARAGSSCYKCGGEGHWARDC--SQSLAPSNQSSSAGSCFKCGKPGHWARDCSN 335
            P     +R  S CY+CG EGH+AR+C  S S++ S+   S   C++C   GH+AR+C N
Sbjct: 344 TPERLFNSREASECYRCGEEGHFARECPNSSSISTSHGRESQTLCYRCNGSGHFARECPN 373

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94C691.0e-0927.91Cold shock domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 GN=CSP3 P... [more]
O656391.9e-0826.25Cold shock protein 1 OS=Arabidopsis thaliana OX=3702 GN=CSP1 PE=1 SV=1[more]
Q048325.6e-0826.92DNA-binding protein HEXBP OS=Leishmania major OX=5664 GN=HEXBP PE=4 SV=1[more]
Q9NG988.1e-0724.35DNA topoisomerase 3-alpha OS=Drosophila melanogaster OX=7227 GN=Top3alpha PE=2 S... [more]
P274841.4e-0644.64Glycine-rich protein 2 OS=Nicotiana sylvestris OX=4096 GN=GRP-2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6577083.16.01e-252100.00DNA topoisomerase 3-alpha, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7015092.11.20e-24799.40DNA topoisomerase 3-alpha, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022931285.13.19e-24598.51cold shock domain-containing protein 3-like [Cucurbita moschata][more]
XP_023552737.13.95e-24194.77cold shock domain-containing protein 3-like [Cucurbita pepo subsp. pepo][more]
XP_022984959.17.61e-23793.91cold shock domain-containing protein 3-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1EZ111.54e-24598.51cold shock domain-containing protein 3-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1JBZ03.68e-23793.91cold shock domain-containing protein 3-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1C4N62.28e-18070.60DNA-binding protein HEXBP-like OS=Momordica charantia OX=3673 GN=LOC111008238 PE... [more]
A0A0A0KVB61.28e-14067.30Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G062410 PE=4 SV=1[more]
A0A2P5AI831.13e-11553.60DNA topoisomerase, type IA OS=Parasponia andersonii OX=3476 GN=PanWU01x14_329900... [more]
Match NameE-valueIdentityDescription
AT3G42860.18.7e-6543.17zinc knuckle (CCHC-type) family protein [more]
AT5G13920.12.3e-1735.17GRF zinc finger / Zinc knuckle protein [more]
AT2G17870.17.3e-1127.91cold shock domain protein 3 [more]
AT4G36020.11.4e-0926.25cold shock domain protein 1 [more]
AT3G43590.12.2e-0722.92zinc knuckle (CCHC-type) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001878Zinc finger, CCHC-typeSMARTSM00343c2hcfinal6coord: 289..305
e-value: 2.8E-5
score: 33.5
coord: 111..127
e-value: 5.7E-5
score: 32.5
coord: 318..334
e-value: 6.4E-6
score: 35.7
IPR001878Zinc finger, CCHC-typePFAMPF00098zf-CCHCcoord: 318..333
e-value: 9.9E-8
score: 31.7
coord: 289..305
e-value: 8.2E-8
score: 32.0
coord: 111..126
e-value: 2.0E-7
score: 30.8
IPR001878Zinc finger, CCHC-typePROSITEPS50158ZF_CCHCcoord: 319..334
score: 11.004309
IPR001878Zinc finger, CCHC-typePROSITEPS50158ZF_CCHCcoord: 290..305
score: 10.674652
IPR001878Zinc finger, CCHC-typePROSITEPS50158ZF_CCHCcoord: 112..125
score: 10.608721
NoneNo IPR availableGENE3D4.10.60.10coord: 314..334
e-value: 2.6E-7
score: 32.2
coord: 282..313
e-value: 5.0E-8
score: 34.5
coord: 104..134
e-value: 3.6E-7
score: 31.7
NoneNo IPR availablePANTHERPTHR33680:SF4ZINC KNUCKLE (CCHC-TYPE) FAMILY PROTEINcoord: 98..207
NoneNo IPR availablePANTHERPTHR33680:SF4ZINC KNUCKLE (CCHC-TYPE) FAMILY PROTEINcoord: 310..332
coord: 203..266
NoneNo IPR availablePANTHERPTHR33680OS07G0190500 PROTEINcoord: 310..332
coord: 203..266
NoneNo IPR availablePANTHERPTHR33680OS07G0190500 PROTEINcoord: 98..207
IPR010666Zinc finger, GRF-typePFAMPF06839zf-GRFcoord: 214..255
e-value: 6.3E-14
score: 51.8
coord: 147..190
e-value: 9.8E-16
score: 57.6
IPR036875Zinc finger, CCHC-type superfamilySUPERFAMILY57756Retrovirus zinc finger-like domainscoord: 105..129
IPR036875Zinc finger, CCHC-type superfamilySUPERFAMILY57756Retrovirus zinc finger-like domainscoord: 286..334

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g242960.m01Csor.00g242960.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding