Csor.00g241490 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g241490
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionEukaryotic aspartyl protease family protein
LocationCsor_Chr04: 13121720 .. 13123096 (-)
RNA-Seq ExpressionCsor.00g241490
SyntenyCsor.00g241490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSsinglepolypeptidestart_codonstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGTGTGTTTCCCAATTCCTCCATCTCACCTTCCTCCTCCTCCTCCTCCTCTCATCCCTCGCAGATCTCTCCAATGCCATTCCCTCTCAATACCTGAAGTTCCCATTACTCCACTCTAACCCCTTCTCCTCCCCTTCTCAAGCCCTCTCCTCCGACACCCACCGCCTCTCCCTCCTCTTCTCCGCCCACCGCCACAGCCCCATCCTCAAGTCCCCCCTCATCTCCGGCGCCTCCACCGGCTCTGGTCAGTACTTCGTCAATCTCCACCTCGGCACCCCTCCTCAAAGCCTCCTCCTAGTCGCCGATACCGGCAGCGACCTCGTCTGGGTCAAATGCTCCCCCTGCCGCAACTGCTCCCACCACCCTCCCTCCTCTGCCTTCTTCCCCCGCCACTCATCCTCCTTCTCCCCTTTCCACTGCTTCGACCCCCACTGCCGCCTCCTTCCCCACCCCCCTTCAAACCGCTGCAACCACACCCACCTCCACTCCCCCTGCCCCTTCCTCTACTCCTACGCCGACTCCTCCCTCTCCTCCGGCTTCTTCTCCAAAGATGTCACCACCTTCAACACCTTCTCCGGGACCCACACCCAGACCCGTCTCAACGACCTTTCCTTTGGATGTGGCTTTCGGATCTCGGGTCCTAGCGTTTCGGGCGCCCGATTCAATGGAGCACGTGGAGTCATGGGATTGGGCAGAGGCCCGATCTCCTTCTCCTCCCAACTCGGCCGCCGATTTGGCAACACTTTTTCTTATTGTCTTATGGATTATACACTCTCTCCGCCGCCCACCAGCTACCTCATGATCGGCGGCGGCCTCCGTAGCCTACCGGTGACAAACGCCTCAAAAATCAGCTACACCCCGTTGCAGATTAACCCTCTGTCCCCGACATTCTACTACATTGTCGTGAAGAGCATCACAGTGGACGGCGTGAAATTGCCCATCAATCCCAAGGTGTGGGCCATCGACGAGCAAGGCAACGGCGGCACGGTGGTGGATTCCGGCACGACATTGACGTATCTAGCTGAGGCAGCATACGAGGAAGTGTTGAAGGCCATGAGACGGCGAGTGAAGCTGCCGAGAGCGCTCCAGTTGAGTCCAGGGTTCGATCTGTGCGTGAACGCATCGGGCGAGTCGCGGATGCGGAGTCTGCCGCGAATAAGATTCCGGGTAGGCGGCGGAGGTGTGTTTGCGCCGCCGGCAAGGAACTATTTTGTGGAGACAGAGGAGGGGGTGATGTGCTTGGCGATCCGACCCGTGGATTCGGGAAATGGGTTTTCGGTGATTGGGAATCTGATGCAACAAGGATTCTTGTTGGAGTTCGATAGAGAGAAGTCGAGGATGGGGTTTTCAAGGCGTGGCTGTGGGCTTCCTTGA

mRNA sequence

ATGCCGTGTGTTTCCCAATTCCTCCATCTCACCTTCCTCCTCCTCCTCCTCCTCTCATCCCTCGCAGATCTCTCCAATGCCATTCCCTCTCAATACCTGAAGTTCCCATTACTCCACTCTAACCCCTTCTCCTCCCCTTCTCAAGCCCTCTCCTCCGACACCCACCGCCTCTCCCTCCTCTTCTCCGCCCACCGCCACAGCCCCATCCTCAAGTCCCCCCTCATCTCCGGCGCCTCCACCGGCTCTGGTCAGTACTTCGTCAATCTCCACCTCGGCACCCCTCCTCAAAGCCTCCTCCTAGTCGCCGATACCGGCAGCGACCTCGTCTGGGTCAAATGCTCCCCCTGCCGCAACTGCTCCCACCACCCTCCCTCCTCTGCCTTCTTCCCCCGCCACTCATCCTCCTTCTCCCCTTTCCACTGCTTCGACCCCCACTGCCGCCTCCTTCCCCACCCCCCTTCAAACCGCTGCAACCACACCCACCTCCACTCCCCCTGCCCCTTCCTCTACTCCTACGCCGACTCCTCCCTCTCCTCCGGCTTCTTCTCCAAAGATGTCACCACCTTCAACACCTTCTCCGGGACCCACACCCAGACCCGTCTCAACGACCTTTCCTTTGGATGTGGCTTTCGGATCTCGGGTCCTAGCGTTTCGGGCGCCCGATTCAATGGAGCACGTGGAGTCATGGGATTGGGCAGAGGCCCGATCTCCTTCTCCTCCCAACTCGGCCGCCGATTTGGCAACACTTTTTCTTATTGTCTTATGGATTATACACTCTCTCCGCCGCCCACCAGCTACCTCATGATCGGCGGCGGCCTCCGTAGCCTACCGGTGACAAACGCCTCAAAAATCAGCTACACCCCGTTGCAGATTAACCCTCTGTCCCCGACATTCTACTACATTGTCGTGAAGAGCATCACAGTGGACGGCGTGAAATTGCCCATCAATCCCAAGGTGTGGGCCATCGACGAGCAAGGCAACGGCGGCACGGTGGTGGATTCCGGCACGACATTGACGTATCTAGCTGAGGCAGCATACGAGGAAGTGTTGAAGGCCATGAGACGGCGAGTGAAGCTGCCGAGAGCGCTCCAGTTGAGTCCAGGGTTCGATCTGTGCGTGAACGCATCGGGCGAGTCGCGGATGCGGAGTCTGCCGCGAATAAGATTCCGGGTAGGCGGCGGAGGTGTGTTTGCGCCGCCGGCAAGGAACTATTTTGTGGAGACAGAGGAGGGGGTGATGTGCTTGGCGATCCGACCCGTGGATTCGGGAAATGGGTTTTCGGTGATTGGGAATCTGATGCAACAAGGATTCTTGTTGGAGTTCGATAGAGAGAAGTCGAGGATGGGGTTTTCAAGGCGTGGCTGTGGGCTTCCTTGA

Coding sequence (CDS)

ATGCCGTGTGTTTCCCAATTCCTCCATCTCACCTTCCTCCTCCTCCTCCTCCTCTCATCCCTCGCAGATCTCTCCAATGCCATTCCCTCTCAATACCTGAAGTTCCCATTACTCCACTCTAACCCCTTCTCCTCCCCTTCTCAAGCCCTCTCCTCCGACACCCACCGCCTCTCCCTCCTCTTCTCCGCCCACCGCCACAGCCCCATCCTCAAGTCCCCCCTCATCTCCGGCGCCTCCACCGGCTCTGGTCAGTACTTCGTCAATCTCCACCTCGGCACCCCTCCTCAAAGCCTCCTCCTAGTCGCCGATACCGGCAGCGACCTCGTCTGGGTCAAATGCTCCCCCTGCCGCAACTGCTCCCACCACCCTCCCTCCTCTGCCTTCTTCCCCCGCCACTCATCCTCCTTCTCCCCTTTCCACTGCTTCGACCCCCACTGCCGCCTCCTTCCCCACCCCCCTTCAAACCGCTGCAACCACACCCACCTCCACTCCCCCTGCCCCTTCCTCTACTCCTACGCCGACTCCTCCCTCTCCTCCGGCTTCTTCTCCAAAGATGTCACCACCTTCAACACCTTCTCCGGGACCCACACCCAGACCCGTCTCAACGACCTTTCCTTTGGATGTGGCTTTCGGATCTCGGGTCCTAGCGTTTCGGGCGCCCGATTCAATGGAGCACGTGGAGTCATGGGATTGGGCAGAGGCCCGATCTCCTTCTCCTCCCAACTCGGCCGCCGATTTGGCAACACTTTTTCTTATTGTCTTATGGATTATACACTCTCTCCGCCGCCCACCAGCTACCTCATGATCGGCGGCGGCCTCCGTAGCCTACCGGTGACAAACGCCTCAAAAATCAGCTACACCCCGTTGCAGATTAACCCTCTGTCCCCGACATTCTACTACATTGTCGTGAAGAGCATCACAGTGGACGGCGTGAAATTGCCCATCAATCCCAAGGTGTGGGCCATCGACGAGCAAGGCAACGGCGGCACGGTGGTGGATTCCGGCACGACATTGACGTATCTAGCTGAGGCAGCATACGAGGAAGTGTTGAAGGCCATGAGACGGCGAGTGAAGCTGCCGAGAGCGCTCCAGTTGAGTCCAGGGTTCGATCTGTGCGTGAACGCATCGGGCGAGTCGCGGATGCGGAGTCTGCCGCGAATAAGATTCCGGGTAGGCGGCGGAGGTGTGTTTGCGCCGCCGGCAAGGAACTATTTTGTGGAGACAGAGGAGGGGGTGATGTGCTTGGCGATCCGACCCGTGGATTCGGGAAATGGGTTTTCGGTGATTGGGAATCTGATGCAACAAGGATTCTTGTTGGAGTTCGATAGAGAGAAGTCGAGGATGGGGTTTTCAAGGCGTGGCTGTGGGCTTCCTTGA

Protein sequence

MPCVSQFLHLTFLLLLLLSSLADLSNAIPSQYLKFPLLHSNPFSSPSQALSSDTHRLSLLFSAHRHSPILKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSNRCNHTHLHSPCPFLYSYADSSLSSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVVKSITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLPRALQLSPGFDLCVNASGESRMRSLPRIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPVDSGNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP
Homology
BLAST of Csor.00g241490 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 4.0e-57
Identity = 139/387 (35.92%), Postives = 203/387 (52.45%), Query Frame = 0

Query: 72  SPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHPPSSAFFPR 131
           S ++SG S GSG+YF  L +GTP + + +V DTGSD+VW++C+PCR C +      F PR
Sbjct: 129 SSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRC-YSQSDPIFDPR 188

Query: 132 HSSSFSPFHCFDPHCRLLPHPPSNRCNHTHLHSPCPFLYSYADSSLSSGFFSKDVTTFNT 191
            S +++   C  PHCR L     N    T     C +  SY D S + G FS +  TF  
Sbjct: 189 KSKTYATIPCSSPHCRRLDSAGCNTRRKT-----CLYQVSYGDGSFTVGDFSTETLTFR- 248

Query: 192 FSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGRRFGNTFS 251
                 + R+  ++ GCG    G       F GA G++GLG+G +SF  Q G RF   FS
Sbjct: 249 ------RNRVKGVALGCGHDNEG------LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFS 308

Query: 252 YCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVVKSITVDGV 311
           YCL+D + S  P+S +     +  +         +TPL  NP   TFYY+ +  I+V G 
Sbjct: 309 YCLVDRSASSKPSSVVFGNAAVSRI-------ARFTPLLSNPKLDTFYYVGLLGISVGGT 368

Query: 312 KLP-INPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVK-LPRALQLSPGF 371
           ++P +   ++ +D+ GNGG ++DSGT++T L   AY  +  A R   K L RA   S  F
Sbjct: 369 RVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSL-F 428

Query: 372 DLCVNASGESRMRSLPRIRFRVGGGGVFAPPARNYFVETE-EGVMCLAIRPVDSGNGFSV 431
           D C + S  + ++ +P +     G  V + PA NY +  +  G  C A     +  G S+
Sbjct: 429 DTCFDLSNMNEVK-VPTVVLHFRGADV-SLPATNYLIPVDTNGKFCFAF--AGTMGGLSI 484

Query: 432 IGNLMQQGFLLEFDREKSRMGFSRRGC 456
           IGN+ QQGF + +D   SR+GF+  GC
Sbjct: 489 IGNIQQQGFRVVYDLASSRVGFAPGGC 484

BLAST of Csor.00g241490 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 1.0e-52
Identity = 132/376 (35.11%), Postives = 191/376 (50.80%), Query Frame = 0

Query: 81  GSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHPPSSAFFPRHSSSFSPFH 140
           G G+Y +N+ +GTP  S   + DTGSDL+W +C PC  C    P+  F P+ SSSFS   
Sbjct: 92  GDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQC-FSQPTPIFNPQDSSSFSTLP 151

Query: 141 CFDPHCRLLPHPPSNRCNHTHLHSPCPFLYSYADSSLSSGFFSKDVTTFNTFSGTHTQTR 200
           C   +C+ L   PS  CN    ++ C + Y Y D S + G+ + +  TF T S       
Sbjct: 152 CESQYCQDL---PSETCN----NNECQYTYGYGDGSTTQGYMATETFTFETSS------- 211

Query: 201 LNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGRRFGNTFSYCLMDYTLS 260
           + +++FGCG    G      + NGA G++G+G GP+S  SQLG      FSYC+  Y  S
Sbjct: 212 VPNIAFGCGEDNQG----FGQGNGA-GLIGMGWGPLSLPSQLG---VGQFSYCMTSYGSS 271

Query: 261 PPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVVKSITVDGVKLPINPKVW 320
            P T  L +G     +P  + S    T L  + L+PT+YYI ++ ITV G  L I    +
Sbjct: 272 SPST--LALGSAASGVPEGSPS----TTLIHSSLNPTYYYITLQGITVGGDNLGIPSSTF 331

Query: 321 AIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLPRALQLSPGFDLCVNASGESR 380
            + + G GG ++DSGTTLTYL + AY  V +A   ++ LP   + S G   C     +  
Sbjct: 332 QLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDESSSGLSTCFQQPSDGS 391

Query: 381 MRSLPRIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPVDSGNGFSVIGNLMQQGFLLE 440
              +P I  +   GGV     +N  +   EGV+CLA+    S  G S+ GN+ QQ   + 
Sbjct: 392 TVQVPEISMQF-DGGVLNLGEQNILISPAEGVICLAMGS-SSQLGISIFGNIQQQETQVL 436

Query: 441 FDREKSRMGFSRRGCG 457
           +D +   + F    CG
Sbjct: 452 YDLQNLAVSFVPTQCG 436

BLAST of Csor.00g241490 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 1.1e-51
Identity = 130/377 (34.48%), Postives = 191/377 (50.66%), Query Frame = 0

Query: 81  GSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHPPSSAFFPRHSSSFSPFH 140
           G G+Y +NL +GTP Q    + DTGSDL+W +C PC  C  +  +  F P+ SSSFS   
Sbjct: 91  GDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQC-FNQSTPIFNPQGSSSFSTLP 150

Query: 141 CFDPHCRLLPHPPSNRCNHTHLHSPCPFLYSYADSSLSSGFFSKDVTTFNTFSGTHTQTR 200
           C    C+ L  P       T  ++ C + Y Y D S + G    +  TF + S       
Sbjct: 151 CSSQLCQALSSP-------TCSNNFCQYTYGYGDGSETQGSMGTETLTFGSVS------- 210

Query: 201 LNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGRRFGNTFSYCLMDYTLS 260
           + +++FGCG    G      + NGA G++G+GRGP+S  SQL       FSYC+     S
Sbjct: 211 IPNITFGCGENNQG----FGQGNGA-GLVGMGRGPLSLPSQLD---VTKFSYCMTPIGSS 270

Query: 261 PPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVVKSITVDGVKLPINPKVW 320
            P  S L++G    S+    A   + T +Q + + PTFYYI +  ++V   +LPI+P  +
Sbjct: 271 TP--SNLLLGSLANSV---TAGSPNTTLIQSSQI-PTFYYITLNGLSVGSTRLPIDPSAF 330

Query: 321 AID-EQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLPRALQLSPGFDLCVNASGES 380
           A++   G GG ++DSGTTLTY    AY+ V +    ++ LP     S GFDLC     + 
Sbjct: 331 ALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSDP 390

Query: 381 RMRSLPRIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPVDSGNGFSVIGNLMQQGFLL 440
               +P       GG +   P+ NYF+    G++CLA+    S  G S+ GN+ QQ  L+
Sbjct: 391 SNLQIPTFVMHFDGGDL-ELPSENYFISPSNGLICLAMG--SSSQGMSIFGNIQQQNMLV 435

Query: 441 EFDREKSRMGFSRRGCG 457
            +D   S + F+   CG
Sbjct: 451 VYDTGNSVVSFASAQCG 435

BLAST of Csor.00g241490 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 4.0e-49
Identity = 131/387 (33.85%), Postives = 198/387 (51.16%), Query Frame = 0

Query: 72  SPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHPPSSAFFPR 131
           S ++SG   GSG+YFV + +G+PP+   +V D+GSD+VWV+C PC+ C +      F P 
Sbjct: 118 SDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLC-YKQSDPVFDPA 177

Query: 132 HSSSFSPFHCFDPHCRLLPHPPSNRCNHTHLHS-PCPFLYSYADSSLSSGFFSKDVTTFN 191
            S S++   C    C        +R  ++  HS  C +   Y D S + G  + +  TF 
Sbjct: 178 KSGSYTGVSCGSSVC--------DRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTF- 237

Query: 192 TFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGRRFGNTF 251
                  +T + +++ GCG R  G       F GA G++G+G G +SF  QL  + G  F
Sbjct: 238 ------AKTVVRNVAMGCGHRNRG------MFIGAAGLLGIGGGSMSFVGQLSGQTGGAF 297

Query: 252 SYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVVKSITVDG 311
            YCL+  +     T  L+   G  +LPV      S+ PL  NP +P+FYY+ +K + V G
Sbjct: 298 GYCLV--SRGTDSTGSLVF--GREALPV----GASWVPLVRNPRAPSFYYVGLKGLGVGG 357

Query: 312 VKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMR-RRVKLPRALQLSPGF 371
           V++P+   V+ + E G+GG V+D+GT +T L  AAY       + +   LPRA  +S  F
Sbjct: 358 VRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSI-F 417

Query: 372 DLCVNASGESRMRSLPRIRFRVGGGGVFAPPARNYFVETEE-GVMCLAIRPVDSGNGFSV 431
           D C + SG   +R +P + F    G V   PARN+ +  ++ G  C A     S  G S+
Sbjct: 418 DTCYDLSGFVSVR-VPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAF--AASPTGLSI 470

Query: 432 IGNLMQQGFLLEFDREKSRMGFSRRGC 456
           IGN+ Q+G  + FD     +GF    C
Sbjct: 478 IGNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of Csor.00g241490 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 2.0e-48
Identity = 125/391 (31.97%), Postives = 196/391 (50.13%), Query Frame = 0

Query: 70  LKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHPPSSAFF 129
           L +P++SGAS GSG+YF  + +GTP + + LV DTGSD+ W++C PC +C +      F 
Sbjct: 147 LTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADC-YQQSDPVFN 206

Query: 130 PRHSSSFSPFHCFDPHCRLLPHPPSNRCNHTHLHSPCPFLYSYADSSLSSGFFSKDVTTF 189
           P  SS++    C  P C LL    ++ C      + C +  SY D S + G  + D  TF
Sbjct: 207 PTSSSTYKSLTCSAPQCSLL---ETSACR----SNKCLYQVSYGDGSFTVGELATDTVTF 266

Query: 190 NTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGRRFGNT 249
              SG     ++N+++ GCG    G       F GA G++GLG G +S ++Q+      +
Sbjct: 267 GN-SG-----KINNVALGCGHDNEG------LFTGAAGLLGLGGGVLSITNQMK---ATS 326

Query: 250 FSYCLMDYTLSPPPT---SYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVVKSI 309
           FSYCL+D       +   + + +GGG  + P+    KI            TFYY+ +   
Sbjct: 327 FSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKID-----------TFYYVGLSGF 386

Query: 310 TVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKA-MRRRVKLPRALQL 369
           +V G K+ +   ++ +D  G+GG ++D GT +T L   AY  +  A ++  V L +    
Sbjct: 387 SVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSS 446

Query: 370 SPGFDLCVNASGESRMRSLPRIRFRVGGGGVFAPPARNYFVETEE-GVMCLAIRPVDSGN 429
              FD C + S  S ++ +P + F   GG     PA+NY +  ++ G  C A  P  S  
Sbjct: 447 ISLFDTCYDFSSLSTVK-VPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSS-- 500

Query: 430 GFSVIGNLMQQGFLLEFDREKSRMGFSRRGC 456
             S+IGN+ QQG  + +D  K+ +G S   C
Sbjct: 507 SLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of Csor.00g241490 vs. NCBI nr
Match: KAG6601756.1 (Aspartic proteinase nepenthesin-2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 928 bits (2399), Expect = 0.0
Identity = 458/458 (100.00%), Postives = 458/458 (100.00%), Query Frame = 0

Query: 1   MPCVSQFLHLTFLLLLLLSSLADLSNAIPSQYLKFPLLHSNPFSSPSQALSSDTHRLSLL 60
           MPCVSQFLHLTFLLLLLLSSLADLSNAIPSQYLKFPLLHSNPFSSPSQALSSDTHRLSLL
Sbjct: 1   MPCVSQFLHLTFLLLLLLSSLADLSNAIPSQYLKFPLLHSNPFSSPSQALSSDTHRLSLL 60

Query: 61  FSAHRHSPILKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCS 120
           FSAHRHSPILKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCS
Sbjct: 61  FSAHRHSPILKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCS 120

Query: 121 HHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSNRCNHTHLHSPCPFLYSYADSSLSSG 180
           HHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSNRCNHTHLHSPCPFLYSYADSSLSSG
Sbjct: 121 HHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSNRCNHTHLHSPCPFLYSYADSSLSSG 180

Query: 181 FFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSS 240
           FFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSS
Sbjct: 181 FFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSS 240

Query: 241 QLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYY 300
           QLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYY
Sbjct: 241 QLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYY 300

Query: 301 IVVKSITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLP 360
           IVVKSITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLP
Sbjct: 301 IVVKSITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLP 360

Query: 361 RALQLSPGFDLCVNASGESRMRSLPRIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPV 420
           RALQLSPGFDLCVNASGESRMRSLPRIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPV
Sbjct: 361 RALQLSPGFDLCVNASGESRMRSLPRIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPV 420

Query: 421 DSGNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP 458
           DSGNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP
Sbjct: 421 DSGNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP 458

BLAST of Csor.00g241490 vs. NCBI nr
Match: KAG7032480.1 (Aspartic proteinase nepenthesin-2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 917 bits (2371), Expect = 0.0
Identity = 453/458 (98.91%), Postives = 455/458 (99.34%), Query Frame = 0

Query: 1   MPCVSQFLHLTFLLLLLLSSLADLSNAIPSQYLKFPLLHSNPFSSPSQALSSDTHRLSLL 60
           M CVSQFLHLTFLLLLLLSSLADLSNAIPSQYLKFPLLHSNPFSSPSQALSSDTHRLSLL
Sbjct: 1   MACVSQFLHLTFLLLLLLSSLADLSNAIPSQYLKFPLLHSNPFSSPSQALSSDTHRLSLL 60

Query: 61  FSAHRHSPILKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCS 120
           FSAHRHSP LKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCS
Sbjct: 61  FSAHRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCS 120

Query: 121 HHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSNRCNHTHLHSPCPFLYSYADSSLSSG 180
           HHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPS+RCNHTHLHSPCPFLYSYADSSLSSG
Sbjct: 121 HHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCPFLYSYADSSLSSG 180

Query: 181 FFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSS 240
           FFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPS SGARFNGARGVMGLGRGPISFSS
Sbjct: 181 FFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSFSGARFNGARGVMGLGRGPISFSS 240

Query: 241 QLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYY 300
           QLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYY
Sbjct: 241 QLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYY 300

Query: 301 IVVKSITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLP 360
           IVVKSITV+GVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLP
Sbjct: 301 IVVKSITVNGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLP 360

Query: 361 RALQLSPGFDLCVNASGESRMRSLPRIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPV 420
           RALQLSPGFDLCVNASGESRMRSLPRIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPV
Sbjct: 361 RALQLSPGFDLCVNASGESRMRSLPRIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPV 420

Query: 421 DSGNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP 458
           DSGNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP
Sbjct: 421 DSGNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP 458

BLAST of Csor.00g241490 vs. NCBI nr
Match: XP_023549731.1 (aspartic proteinase nepenthesin-2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 906 bits (2342), Expect = 0.0
Identity = 448/463 (96.76%), Postives = 454/463 (98.06%), Query Frame = 0

Query: 1   MPCVSQFLHLTF-----LLLLLLSSLADLSNAIPSQYLKFPLLHSNPFSSPSQALSSDTH 60
           MPCVSQFLHLTF     LLLLLLSSL D SNAIPSQYLKFPLLH+NPFSSPSQALSSDTH
Sbjct: 1   MPCVSQFLHLTFFLLLLLLLLLLSSLTDPSNAIPSQYLKFPLLHTNPFSSPSQALSSDTH 60

Query: 61  RLSLLFSAHRHSPILKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSP 120
           RLSLLFS+HRHSP LKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSP
Sbjct: 61  RLSLLFSSHRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSP 120

Query: 121 CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSNRCNHTHLHSPCPFLYSYADS 180
           CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPS+RCNHTHLHSPCPFLYSYADS
Sbjct: 121 CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCPFLYSYADS 180

Query: 181 SLSSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGP 240
           SLSSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGP
Sbjct: 181 SLSSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGP 240

Query: 241 ISFSSQLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLS 300
           ISFSSQLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLS
Sbjct: 241 ISFSSQLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLS 300

Query: 301 PTFYYIVVKSITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRR 360
           PTFYYIVVKSITVDGVKLPINP VWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRR
Sbjct: 301 PTFYYIVVKSITVDGVKLPINPNVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRR 360

Query: 361 RVKLPRALQLSPGFDLCVNASGESRMRSLPRIRFRVGGGGVFAPPARNYFVETEEGVMCL 420
           RVKLPRALQLSPGFDLCVNASGESRMRSLP++RFRVGGGGVFAPPARNYFVETEEGVMCL
Sbjct: 361 RVKLPRALQLSPGFDLCVNASGESRMRSLPQLRFRVGGGGVFAPPARNYFVETEEGVMCL 420

Query: 421 AIRPVDSGNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP 458
           AIRPVDSGNGFSVIGNLMQQGFLLEFDR+KSRMGFSRRGCGLP
Sbjct: 421 AIRPVDSGNGFSVIGNLMQQGFLLEFDRDKSRMGFSRRGCGLP 463

BLAST of Csor.00g241490 vs. NCBI nr
Match: XP_022930101.1 (aspartyl protease family protein 2-like [Cucurbita moschata])

HSP 1 Score: 894 bits (2311), Expect = 0.0
Identity = 444/458 (96.94%), Postives = 447/458 (97.60%), Query Frame = 0

Query: 1   MPCVSQFLHLTFLLLLLLSSLADLSNAIPSQYLKFPLLHSNPFSSPSQALSSDTHRLSLL 60
           MPCV QFL    LLLLLLSSLADLSNAIPSQYLKFPLLH+NPFSSPSQALSSDTHRLSLL
Sbjct: 1   MPCVPQFL---LLLLLLLSSLADLSNAIPSQYLKFPLLHTNPFSSPSQALSSDTHRLSLL 60

Query: 61  FSAHRHSPILKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCS 120
           FSAHRHSP LKSPLISGASTGSGQYFVNLHLGTPPQSLLLV DTGSDLVWVKCSPCRNCS
Sbjct: 61  FSAHRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVVDTGSDLVWVKCSPCRNCS 120

Query: 121 HHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSNRCNHTHLHSPCPFLYSYADSSLSSG 180
           HHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPS+RCNHTHLHSPC FLYSYADSSLSSG
Sbjct: 121 HHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCSFLYSYADSSLSSG 180

Query: 181 FFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSS 240
           FFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARF GARGVMGLGRGPISFSS
Sbjct: 181 FFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFTGARGVMGLGRGPISFSS 240

Query: 241 QLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYY 300
           QLG RFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYY
Sbjct: 241 QLGHRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYY 300

Query: 301 IVVKSITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLP 360
           IVVKSITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLP
Sbjct: 301 IVVKSITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLP 360

Query: 361 RALQLSPGFDLCVNASGESRMRSLPRIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPV 420
           RALQLSPGFDLCVNAS ESRMRSLP+IRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPV
Sbjct: 361 RALQLSPGFDLCVNASSESRMRSLPQIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPV 420

Query: 421 DSGNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP 458
           DSGNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP
Sbjct: 421 DSGNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP 455

BLAST of Csor.00g241490 vs. NCBI nr
Match: XP_022969957.1 (aspartyl protease family protein 2 [Cucurbita maxima])

HSP 1 Score: 754 bits (1947), Expect = 5.14e-272
Identity = 382/461 (82.86%), Postives = 406/461 (88.07%), Query Frame = 0

Query: 1   MPCVSQFLHLTFLLLLLLSSLADLSNAIPS-QYLKFPLLHSNPFSSPSQALSSDTHRLSL 60
           M  VS F HL    +LLL SLADL NA  +  YLK PLLH NPFSSPSQALSSDTHRLSL
Sbjct: 5   MLSVSTFFHL----ILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSL 64

Query: 61  LFSA--HRHSPILKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCR 120
           LFSA   R +P LKSPLISGASTGSGQYFV+L +GTPPQSLLLVADTGSDLVWVKCS CR
Sbjct: 65  LFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACR 124

Query: 121 NCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSNRCNHTHLHSPCPFLYSYADSSL 180
           NCSHHPPSSAF PRHSSSFSPFHCFDPHCRLLPH P + CNHT LHSPC FLY+YAD S 
Sbjct: 125 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGST 184

Query: 181 SSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPIS 240
           SSGFFSK+ TT  T SG+  +TRL DLSFGCGFRISGPSVSGA+FNGARGVMGLGRGPIS
Sbjct: 185 SSGFFSKETTTLKTLSGS--ETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPIS 244

Query: 241 FSSQLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPT 300
           FS+QLGRRFGN FSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNA+KISYTPL INPLSPT
Sbjct: 245 FSTQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPT 304

Query: 301 FYYIVVKSITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRV 360
           FYYI VKSITVDGVKLPINP VWAIDEQGNGGTVVDSGTTLTYLAE AY+EVLKA+R+RV
Sbjct: 305 FYYIAVKSITVDGVKLPINPTVWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAVRQRV 364

Query: 361 KLPRALQLSPGFDLCVNASGESRMRSLPRIRFRVGGGGVFAPPARNYFVETEEGVMCLAI 420
           KLP A +L+PGFDLCVN S ES+  SLPR+RFRVG G VFAPPARNYF+ET EGVMCLAI
Sbjct: 365 KLPAAAELTPGFDLCVNVSKESQRPSLPRVRFRVGNGAVFAPPARNYFLETVEGVMCLAI 424

Query: 421 RPVDSGNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP 458
           R V+ GNGFSVIGNLMQQGFLLEFD+E SR+GFSRRGCGLP
Sbjct: 425 RAVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

BLAST of Csor.00g241490 vs. ExPASy TrEMBL
Match: A0A6J1EPZ3 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111436585 PE=3 SV=1)

HSP 1 Score: 894 bits (2311), Expect = 0.0
Identity = 444/458 (96.94%), Postives = 447/458 (97.60%), Query Frame = 0

Query: 1   MPCVSQFLHLTFLLLLLLSSLADLSNAIPSQYLKFPLLHSNPFSSPSQALSSDTHRLSLL 60
           MPCV QFL    LLLLLLSSLADLSNAIPSQYLKFPLLH+NPFSSPSQALSSDTHRLSLL
Sbjct: 1   MPCVPQFL---LLLLLLLSSLADLSNAIPSQYLKFPLLHTNPFSSPSQALSSDTHRLSLL 60

Query: 61  FSAHRHSPILKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCS 120
           FSAHRHSP LKSPLISGASTGSGQYFVNLHLGTPPQSLLLV DTGSDLVWVKCSPCRNCS
Sbjct: 61  FSAHRHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVVDTGSDLVWVKCSPCRNCS 120

Query: 121 HHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSNRCNHTHLHSPCPFLYSYADSSLSSG 180
           HHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPS+RCNHTHLHSPC FLYSYADSSLSSG
Sbjct: 121 HHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCSFLYSYADSSLSSG 180

Query: 181 FFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSS 240
           FFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARF GARGVMGLGRGPISFSS
Sbjct: 181 FFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFTGARGVMGLGRGPISFSS 240

Query: 241 QLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYY 300
           QLG RFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYY
Sbjct: 241 QLGHRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYY 300

Query: 301 IVVKSITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLP 360
           IVVKSITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLP
Sbjct: 301 IVVKSITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLP 360

Query: 361 RALQLSPGFDLCVNASGESRMRSLPRIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPV 420
           RALQLSPGFDLCVNAS ESRMRSLP+IRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPV
Sbjct: 361 RALQLSPGFDLCVNASSESRMRSLPQIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPV 420

Query: 421 DSGNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP 458
           DSGNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP
Sbjct: 421 DSGNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP 455

BLAST of Csor.00g241490 vs. ExPASy TrEMBL
Match: A0A6J1HXS2 (aspartyl protease family protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111469001 PE=3 SV=1)

HSP 1 Score: 754 bits (1947), Expect = 2.49e-272
Identity = 382/461 (82.86%), Postives = 406/461 (88.07%), Query Frame = 0

Query: 1   MPCVSQFLHLTFLLLLLLSSLADLSNAIPS-QYLKFPLLHSNPFSSPSQALSSDTHRLSL 60
           M  VS F HL    +LLL SLADL NA  +  YLK PLLH NPFSSPSQALSSDTHRLSL
Sbjct: 5   MLSVSTFFHL----ILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSL 64

Query: 61  LFSA--HRHSPILKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCR 120
           LFSA   R +P LKSPLISGASTGSGQYFV+L +GTPPQSLLLVADTGSDLVWVKCS CR
Sbjct: 65  LFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACR 124

Query: 121 NCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSNRCNHTHLHSPCPFLYSYADSSL 180
           NCSHHPPSSAF PRHSSSFSPFHCFDPHCRLLPH P + CNHT LHSPC FLY+YAD S 
Sbjct: 125 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGST 184

Query: 181 SSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPIS 240
           SSGFFSK+ TT  T SG+  +TRL DLSFGCGFRISGPSVSGA+FNGARGVMGLGRGPIS
Sbjct: 185 SSGFFSKETTTLKTLSGS--ETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPIS 244

Query: 241 FSSQLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPT 300
           FS+QLGRRFGN FSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNA+KISYTPL INPLSPT
Sbjct: 245 FSTQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPT 304

Query: 301 FYYIVVKSITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRV 360
           FYYI VKSITVDGVKLPINP VWAIDEQGNGGTVVDSGTTLTYLAE AY+EVLKA+R+RV
Sbjct: 305 FYYIAVKSITVDGVKLPINPTVWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAVRQRV 364

Query: 361 KLPRALQLSPGFDLCVNASGESRMRSLPRIRFRVGGGGVFAPPARNYFVETEEGVMCLAI 420
           KLP A +L+PGFDLCVN S ES+  SLPR+RFRVG G VFAPPARNYF+ET EGVMCLAI
Sbjct: 365 KLPAAAELTPGFDLCVNVSKESQRPSLPRVRFRVGNGAVFAPPARNYFLETVEGVMCLAI 424

Query: 421 RPVDSGNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP 458
           R V+ GNGFSVIGNLMQQGFLLEFD+E SR+GFSRRGCGLP
Sbjct: 425 RAVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

BLAST of Csor.00g241490 vs. ExPASy TrEMBL
Match: A0A6J1ELQ4 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111435697 PE=3 SV=1)

HSP 1 Score: 750 bits (1937), Expect = 8.29e-271
Identity = 378/461 (82.00%), Postives = 405/461 (87.85%), Query Frame = 0

Query: 1   MPCVSQFLHLTFLLLLLLSSLADLSNAIPS-QYLKFPLLHSNPFSSPSQALSSDTHRLSL 60
           M  VS F HL    +LLL SLADL NA  +  YLK PLLH NPFSSPSQALSSDTHRLSL
Sbjct: 5   MLSVSPFFHL----ILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSL 64

Query: 61  LFSA--HRHSPILKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCR 120
           LFSA   R +P LKSPLISGASTGSGQYFV+L +GTPPQSLLLVADTGSDLVWVKCS CR
Sbjct: 65  LFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACR 124

Query: 121 NCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHPPSNRCNHTHLHSPCPFLYSYADSSL 180
           NCSHHPPSSAF PRHSSSFSPFHCFDPHCRLLPH P + CNHT LHSPC FLY+YAD S 
Sbjct: 125 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGST 184

Query: 181 SSGFFSKDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPIS 240
           SSGFFSK+ TT  T +G+  +TRL DLSFGCGFRISGPSVSGA+FNGARGVMGLGRGPIS
Sbjct: 185 SSGFFSKETTTLKTLTGS--ETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPIS 244

Query: 241 FSSQLGRRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPT 300
           FS+QLGRRFGN FSYCLMDYTLSPPPTSYLMIGGGLR LPVTNA+KISYTPL INPLSPT
Sbjct: 245 FSTQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPT 304

Query: 301 FYYIVVKSITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRV 360
           FYYI VKSITVDGVKLPINP +WAIDEQGNGGTVVDSGTTLTYLAE AY+EVLKAMR+RV
Sbjct: 305 FYYIAVKSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRV 364

Query: 361 KLPRALQLSPGFDLCVNASGESRMRSLPRIRFRVGGGGVFAPPARNYFVETEEGVMCLAI 420
           KLP A +L+PGFDLCVN S ES+  SLPR+RF++G G VF PPARNYF+ETEEGVMCLAI
Sbjct: 365 KLPAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAI 424

Query: 421 RPVDSGNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP 458
           R V+ GNGFSVIGNLMQQGFLLEFD+E SR+GFSRRGCGLP
Sbjct: 425 RAVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

BLAST of Csor.00g241490 vs. ExPASy TrEMBL
Match: A0A0A0KNH6 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G174650 PE=3 SV=1)

HSP 1 Score: 733 bits (1893), Expect = 4.15e-264
Identity = 367/454 (80.84%), Postives = 398/454 (87.67%), Query Frame = 0

Query: 12  FLLLLLLSS-LADLSN------AIPSQYLKFPLLHSNPFSSPSQALSSDTHRLSLLFSAH 71
           FLL+LL S  L  L N      A P+ +LK PLLH  PFSSPSQ+LSSDTHRLSLLFS  
Sbjct: 10  FLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTHRLSLLFS-- 69

Query: 72  RHSPILKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHPP 131
           R +P LKSPLISGASTGSGQYFV++ LGTPPQSLLLVADTGSDLVWVKCS CRNCSHHPP
Sbjct: 70  RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPP 129

Query: 132 SSAFFPRHSSSFSPFHCFDPHCRLLPHPPSNRCNHTHLHSPCPFLYSYADSSLSSGFFSK 191
           SSAF PRHSSSFSPFHCFDPHCRLLPH P + CNHT LHSPC FLYSYAD SLSSGFFSK
Sbjct: 130 SSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSLSSGFFSK 189

Query: 192 DVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGR 251
           + TT  + SG+  +  L  LSFGCGFRISGPSVSGA+FNGARGVMGLGRG ISFSSQLGR
Sbjct: 190 ETTTLKSLSGS--EIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGR 249

Query: 252 RFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVVK 311
           RFGN FSYCLMDYTLSPPPTS+LMIGGGL SLP+TNA+KISYTPLQINPLSPTFYYI + 
Sbjct: 250 RFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYITIH 309

Query: 312 SITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLPRALQ 371
           SIT+DGVKLPINP VW IDEQGNGGTVVDSGTTLTYL + AYEEVLK++RRRVKLP A +
Sbjct: 310 SITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAAE 369

Query: 372 LSPGFDLCVNASGESRMRSLPRIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPVDSGN 431
           L+PGFDLCVNASGESR  SLPR+RFR+GGG VFAPP RNYF+ETEEGVMCLAIR V+SGN
Sbjct: 370 LTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVESGN 429

Query: 432 GFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP 458
           GFSVIGNLMQQGFLLEFD+E+SR+GF+RRGCGLP
Sbjct: 430 GFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

BLAST of Csor.00g241490 vs. ExPASy TrEMBL
Match: A0A1S3CSZ8 (aspartyl protease family protein 2 OS=Cucumis melo OX=3656 GN=LOC103504612 PE=3 SV=1)

HSP 1 Score: 733 bits (1892), Expect = 5.89e-264
Identity = 359/432 (83.10%), Postives = 387/432 (89.58%), Query Frame = 0

Query: 27  AIPSQYLKFPLLHSNPFSSPSQALSSDTHRLSLLFSAHRHSPILKSPLISGASTGSGQYF 86
           A  + +LK PLLH  PFSSPSQ+LSSDTHRLSLLFS  R +P LKSPLISGASTGSGQYF
Sbjct: 32  AAAADFLKLPLLHKPPFSSPSQSLSSDTHRLSLLFS--RPNPTLKSPLISGASTGSGQYF 91

Query: 87  VNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHPPSSAFFPRHSSSFSPFHCFDPHC 146
           V++ LGTPPQSLLLVADTGSDLVWVKCS CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHC
Sbjct: 92  VDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFFPRHSSSFSPFHCFDPHC 151

Query: 147 RLLPHPPSNRCNHTHLHSPCPFLYSYADSSLSSGFFSKDVTTFNTFSGTHTQTRLNDLSF 206
           RLLPH P + CNHT LHSPC FLYSYAD SLSSGFFSK+ TT  T SG+  +  L  LSF
Sbjct: 152 RLLPHAPPHHCNHTLLHSPCRFLYSYADGSLSSGFFSKETTTLKTLSGS--EIHLKGLSF 211

Query: 207 GCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGRRFGNTFSYCLMDYTLSPPPTSY 266
           GCGFRISGPSVSGA+FNGARGVMGLGRG ISFSSQLGRRFGN FSYCLMDYTLSPPPTS+
Sbjct: 212 GCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGRRFGNKFSYCLMDYTLSPPPTSF 271

Query: 267 LMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVVKSITVDGVKLPINPKVWAIDEQG 326
           LMIGGGL SLPV NA+KISYTPLQINPLSPTFYYI + SIT+DGVKLPINP VW IDEQG
Sbjct: 272 LMIGGGLHSLPVNNATKISYTPLQINPLSPTFYYITINSITIDGVKLPINPAVWEIDEQG 331

Query: 327 NGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLPRALQLSPGFDLCVNASGESRMRSLPR 386
           NGGTVVDSGTTLTYL + AYEEVLK++RRRVKLP A +L+PGFDLCVNASGESR  SLPR
Sbjct: 332 NGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAAELTPGFDLCVNASGESRRPSLPR 391

Query: 387 IRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPVDSGNGFSVIGNLMQQGFLLEFDREKS 446
           +RFR+GGG VFAPP RNYF+ETEEGVMCLAIR V+SGNGFSVIGNLMQQGFLLEFD+E+S
Sbjct: 392 LRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVESGNGFSVIGNLMQQGFLLEFDKEES 451

Query: 447 RMGFSRRGCGLP 458
           R+GF+RRGCGLP
Sbjct: 452 RLGFTRRGCGLP 459

BLAST of Csor.00g241490 vs. TAIR 10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 574.3 bits (1479), Expect = 8.6e-164
Identity = 289/456 (63.38%), Postives = 350/456 (76.75%), Query Frame = 0

Query: 7   FLHLTFLLLLLL--SSLADLSNAIPSQYLKFPLLHSNPFSSPSQALSSDTHRLSLLFSAH 66
           F   +FL L LL  S++A +SN   ++YLK PLL  +PF SP+QAL+ DT RL  L    
Sbjct: 6   FFLCSFLSLFLLPPSNIAAVSN--HNKYLKLPLLRKSPFPSPTQALALDTRRLHFLSLRR 65

Query: 67  RHSPILKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHPP 126
           +  P +KSP++SGA++GSGQYFV+L +G PPQSLLL+ADTGSDLVWVKCS CRNCSHH P
Sbjct: 66  KPIPFVKSPVVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKCSACRNCSHHSP 125

Query: 127 SSAFFPRHSSSFSPFHCFDPHCRLLPHPP-SNRCNHTHLHSPCPFLYSYADSSLSSGFFS 186
           ++ FFPRHSS+FSP HC+DP CRL+P P  +  CNHT +HS C + Y YAD SL+SG F+
Sbjct: 126 ATVFFPRHSSTFSPAHCYDPVCRLVPKPDRAPICNHTRIHSTCHYEYGYADGSLTSGLFA 185

Query: 187 KDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLG 246
           ++ T+  T SG   + RL  ++FGCGFRISG SVSG  FNGA GVMGLGRGPISF+SQLG
Sbjct: 186 RETTSLKTSSG--KEARLKSVAFGCGFRISGQSVSGTSFNGANGVMGLGRGPISFASQLG 245

Query: 247 RRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVV 306
           RRFGN FSYCLMDYTLSPPPTSYL+IG G   +     SK+ +TPL  NPLSPTFYY+ +
Sbjct: 246 RRFGNKFSYCLMDYTLSPPPTSYLIIGNGGDGI-----SKLFFTPLLTNPLSPTFYYVKL 305

Query: 307 KSITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLPRAL 366
           KS+ V+G KL I+P +W ID+ GNGGTVVDSGTTL +LAE AY  V+ A+RRRVKLP A 
Sbjct: 306 KSVFVNGAKLRIDPSIWEIDDSGNGGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLPIAD 365

Query: 367 QLSPGFDLCVNASGESR-MRSLPRIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPVDS 426
            L+PGFDLCVN SG ++  + LPR++F   GG VF PP RNYF+ETEE + CLAI+ VD 
Sbjct: 366 ALTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPPPRNYFIETEEQIQCLAIQSVDP 425

Query: 427 GNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP 459
             GFSVIGNLMQQGFL EFDR++SR+GFSRRGC LP
Sbjct: 426 KVGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGCALP 452

BLAST of Csor.00g241490 vs. TAIR 10
Match: AT3G25700.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 383.3 bits (983), Expect = 2.8e-106
Identity = 217/456 (47.59%), Postives = 266/456 (58.33%), Query Frame = 0

Query: 7   FLHLTFLLLLLL--SSLADLSNAIPSQYLKFPLLHSNPFSSPSQALSSDTHRLSLLFSAH 66
           F   +FL L LL  S++A +SN   ++YLK PLL  +PF SP+QAL+ DT RL  L    
Sbjct: 6   FFLCSFLSLFLLPPSNIAAVSN--HNKYLKLPLLRKSPFPSPTQALALDTRRLHFLSLRR 65

Query: 67  RHSPILKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHPP 126
           +  P +KSP++SGA++GSGQYFV+L +G PPQSLLL+ADTGSDLVWVKCS CRNCSHH P
Sbjct: 66  KPIPFVKSPVVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKCSACRNCSHHSP 125

Query: 127 SSAFFPRHSSSFSPFHCFDPHCRLLPHPP-SNRCNHTHLHSPCPFLYSYADSSLSSGFFS 186
           ++ FFPRHSS+FSP HC+DP CRL+P P  +  CNHT +HS C + Y YAD SL+SG F+
Sbjct: 126 ATVFFPRHSSTFSPAHCYDPVCRLVPKPDRAPICNHTRIHSTCHYEYGYADGSLTSGLFA 185

Query: 187 KDVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLG 246
           ++ T+  T SG   + RL  ++FGCGFRISG SVS                         
Sbjct: 186 RETTSLKTSSG--KEARLKSVAFGCGFRISGQSVS------------------------- 245

Query: 247 RRFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVV 306
                                                                       
Sbjct: 246 ------------------------------------------------------------ 305

Query: 307 KSITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLPRAL 366
                                 GNGGTVVDSGTTL +LAE AY  V+ A+RRRVKLP A 
Sbjct: 306 ----------------------GNGGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLPIAD 350

Query: 367 QLSPGFDLCVNASGESR-MRSLPRIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPVDS 426
            L+PGFDLCVN SG ++  + LPR++F   GG VF PP RNYF+ETEE + CLAI+ VD 
Sbjct: 366 ALTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPPPRNYFIETEEQIQCLAIQSVDP 350

Query: 427 GNGFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP 459
             GFSVIGNLMQQGFL EFDR++SR+GFSRRGC LP
Sbjct: 426 KVGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGCALP 350

BLAST of Csor.00g241490 vs. TAIR 10
Match: AT2G42980.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 247.3 bits (630), Expect = 2.4e-65
Identity = 152/392 (38.78%), Postives = 220/392 (56.12%), Query Frame = 0

Query: 70  LKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHPPSSAFF 129
           L + L SG + GSG+YF+++ +GTPP+   L+ DTGSDL W++C PC +C H   +  F+
Sbjct: 145 LIATLESGMTLGSGEYFMDVLVGTPPKHFSLILDTGSDLNWLQCLPCYDCFHQ--NGMFY 204

Query: 130 -PRHSSSFSPFHCFDPHCRLLPHP-PSNRCNHTHLHSPCPFLYSYADSSLSSGFFSKDVT 189
            P+ S+SF    C DP C L+  P P  +C     +  CP+ Y Y D S ++G F+ +  
Sbjct: 205 DPKTSASFKNITCNDPRCSLISSPDPPVQCESD--NQSCPYFYWYGDRSNTTGDFAVETF 264

Query: 190 TFN--TFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGRR 249
           T N  T  G  ++ ++ ++ FGCG    G       F+GA G++GLGRGP+SFSSQL   
Sbjct: 265 TVNLTTTEGGSSEYKVGNMMFGCGHWNRG------LFSGASGLLGLGRGPLSFSSQLQSL 324

Query: 250 FGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVVKS 309
           +G++FSYCL+D   +   +S L+ G     L  TN +  S+   + N +  TFYYI +KS
Sbjct: 325 YGHSFSYCLVDRNSNTNVSSKLIFGEDKDLLNHTNLNFTSFVNGKENSVE-TFYYIQIKS 384

Query: 310 ITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLPRAL-Q 369
           I V G  L I  + W I   G+GGT++DSGTTL+Y AE AYE +      ++K    + +
Sbjct: 385 ILVGGKALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPAYEIIKNKFAEKMKENYPIFR 444

Query: 370 LSPGFDLCVNASG-ESRMRSLPRIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPVDSG 429
             P  D C N SG E     LP +      G V+  PA N F+   E ++CLAI      
Sbjct: 445 DFPVLDPCFNVSGIEENNIHLPELGIAFVDGTVWNFPAENSFIWLSEDLVCLAILGTPKS 504

Query: 430 NGFSVIGNLMQQGFLLEFDREKSRMGFSRRGC 456
             FS+IGN  QQ F + +D ++SR+GF+   C
Sbjct: 505 T-FSIIGNYQQQNFHILYDTKRSRLGFTPTKC 524

BLAST of Csor.00g241490 vs. TAIR 10
Match: AT3G59080.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 233.8 bits (595), Expect = 2.8e-61
Identity = 145/391 (37.08%), Postives = 214/391 (54.73%), Query Frame = 0

Query: 70  LKSPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHPPSSAFF 129
           L + L SG + GSG+YF+++ +G+PP+   L+ DTGSDL W++C PC +C     + AF+
Sbjct: 155 LVATLESGMTLGSGEYFMDVLVGSPPKHFSLILDTGSDLNWIQCLPCYDCFQQ--NGAFY 214

Query: 130 -PRHSSSFSPFHCFDPHCRLLPHP-PSNRCNHTHLHSPCPFLYSYADSSLSSGFFSKDVT 189
            P+ S+S+    C D  C L+  P P   C     +  CP+ Y Y DSS ++G F+ +  
Sbjct: 215 DPKASASYKNITCNDQRCNLVSSPDPPMPCKSD--NQSCPYYYWYGDSSNTTGDFAVETF 274

Query: 190 TFN--TFSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGRR 249
           T N  T  G+     + ++ FGCG    G       F+GA G++GLGRGP+SFSSQL   
Sbjct: 275 TVNLTTNGGSSELYNVENMMFGCGHWNRG------LFHGAAGLLGLGRGPLSFSSQLQSL 334

Query: 250 FGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVVKS 309
           +G++FSYCL+D       +S L+ G     L   N +  S+   + N L  TFYY+ +KS
Sbjct: 335 YGHSFSYCLVDRNSDTNVSSKLIFGEDKDLLSHPNLNFTSFVAGKEN-LVDTFYYVQIKS 394

Query: 310 ITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLPRALQL 369
           I V G  L I  + W I   G GGT++DSGTTL+Y AE AYE +   +  + K    +  
Sbjct: 395 ILVAGEVLNIPEETWNISSDGAGGTIIDSGTTLSYFAEPAYEFIKNKIAEKAKGKYPVYR 454

Query: 370 S-PGFDLCVNASGESRMRSLPRIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPVDSGN 429
             P  D C N SG   ++ LP +      G V+  P  N F+   E ++CLA+      +
Sbjct: 455 DFPILDPCFNVSGIHNVQ-LPELGIAFADGAVWNFPTENSFIWLNEDLVCLAMLGTPK-S 514

Query: 430 GFSVIGNLMQQGFLLEFDREKSRMGFSRRGC 456
            FS+IGN  QQ F + +D ++SR+G++   C
Sbjct: 515 AFSIIGNYQQQNFHILYDTKRSRLGYAPTKC 532

BLAST of Csor.00g241490 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 223.8 bits (569), Expect = 2.9e-58
Identity = 139/387 (35.92%), Postives = 203/387 (52.45%), Query Frame = 0

Query: 72  SPLISGASTGSGQYFVNLHLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHPPSSAFFPR 131
           S ++SG S GSG+YF  L +GTP + + +V DTGSD+VW++C+PCR C +      F PR
Sbjct: 129 SSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRC-YSQSDPIFDPR 188

Query: 132 HSSSFSPFHCFDPHCRLLPHPPSNRCNHTHLHSPCPFLYSYADSSLSSGFFSKDVTTFNT 191
            S +++   C  PHCR L     N    T     C +  SY D S + G FS +  TF  
Sbjct: 189 KSKTYATIPCSSPHCRRLDSAGCNTRRKT-----CLYQVSYGDGSFTVGDFSTETLTFR- 248

Query: 192 FSGTHTQTRLNDLSFGCGFRISGPSVSGARFNGARGVMGLGRGPISFSSQLGRRFGNTFS 251
                 + R+  ++ GCG    G       F GA G++GLG+G +SF  Q G RF   FS
Sbjct: 249 ------RNRVKGVALGCGHDNEG------LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFS 308

Query: 252 YCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVVKSITVDGV 311
           YCL+D + S  P+S +     +  +         +TPL  NP   TFYY+ +  I+V G 
Sbjct: 309 YCLVDRSASSKPSSVVFGNAAVSRI-------ARFTPLLSNPKLDTFYYVGLLGISVGGT 368

Query: 312 KLP-INPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVK-LPRALQLSPGF 371
           ++P +   ++ +D+ GNGG ++DSGT++T L   AY  +  A R   K L RA   S  F
Sbjct: 369 RVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSL-F 428

Query: 372 DLCVNASGESRMRSLPRIRFRVGGGGVFAPPARNYFVETE-EGVMCLAIRPVDSGNGFSV 431
           D C + S  + ++ +P +     G  V + PA NY +  +  G  C A     +  G S+
Sbjct: 429 DTCFDLSNMNEVK-VPTVVLHFRGADV-SLPATNYLIPVDTNGKFCFAF--AGTMGGLSI 484

Query: 432 IGNLMQQGFLLEFDREKSRMGFSRRGC 456
           IGN+ QQGF + +D   SR+GF+  GC
Sbjct: 489 IGNIQQQGFRVVYDLASSRVGFAPGGC 484

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LNJ34.0e-5735.92Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q766C21.0e-5235.11Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Q766C31.1e-5134.48Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q9LHE34.0e-4933.85Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LS402.0e-4831.97Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Match NameE-valueIdentityDescription
KAG6601756.10.0100.00Aspartic proteinase nepenthesin-2, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7032480.10.098.91Aspartic proteinase nepenthesin-2, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_023549731.10.096.76aspartic proteinase nepenthesin-2-like [Cucurbita pepo subsp. pepo][more]
XP_022930101.10.096.94aspartyl protease family protein 2-like [Cucurbita moschata][more]
XP_022969957.15.14e-27282.86aspartyl protease family protein 2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1EPZ30.096.94aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1HXS22.49e-27282.86aspartyl protease family protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111469001 P... [more]
A0A6J1ELQ48.29e-27182.00aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A0A0KNH64.15e-26480.84Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G17465... [more]
A0A1S3CSZ85.89e-26483.10aspartyl protease family protein 2 OS=Cucumis melo OX=3656 GN=LOC103504612 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT3G25700.18.6e-16463.38Eukaryotic aspartyl protease family protein [more]
AT3G25700.22.8e-10647.59Eukaryotic aspartyl protease family protein [more]
AT2G42980.12.4e-6538.78Eukaryotic aspartyl protease family protein [more]
AT3G59080.12.8e-6137.08Eukaryotic aspartyl protease family protein [more]
AT1G01300.12.9e-5835.92Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 427..442
score: 18.99
coord: 91..111
score: 49.53
coord: 330..341
score: 41.24
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 62..270
e-value: 1.0E-46
score: 161.3
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 275..456
e-value: 7.9E-52
score: 177.6
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 79..455
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 298..451
e-value: 2.8E-36
score: 124.7
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 85..271
e-value: 1.0E-47
score: 162.6
NoneNo IPR availablePANTHERPTHR47967OS07G0603500 PROTEIN-RELATEDcoord: 11..456
NoneNo IPR availablePANTHERPTHR47967:SF72BNAA02G27600D PROTEINcoord: 11..456
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 100..111
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 85..451
score: 39.44825
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 84..455
e-value: 9.44008E-86
score: 261.814

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g241490.m01Csor.00g241490.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005576 extracellular region
molecular_function GO:0004190 aspartic-type endopeptidase activity