Csor.00g238520 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g238520
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	RNA-dependent RNA polymerase
Location	Csor_Chr10: 6090722 .. 6091480 (+)
RNA-Seq Expression	Csor.00g238520
Synteny	Csor.00g238520

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codonsinglepolypeptidestop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCAAATTCAGCAGCAGTCGCTACCGTCTATGCAAAGAAGAACACGATTATCTTTGGATTCGGACAACAGAGTTTTCTTCTCTGAGATCAATAGGTCAGTCCACTTCCTTTTGTTGGGAAGTTGAAGAAGATTTGCAGGCTTCAGATATCTTTTCTAGTTTCCCACTTTACAGGGAAACTCCAAAAGATATTACATTAGAGGATGGTGGAGAATTTGGCTCTACCTCAGAGGCTGTTCCTCTGATAAAATCTGGGTTAGTTTCTAATCTAGCTTATGAGGTTCAATATCAGCTCAATTCCTTAGTTCATATGCAGAAAATTAGCCTTGCTGCAGCCAGCAATGATCTGATTGAATTCCTTAGCACTTTAGGTACTGACACTGCGTTGAATATACTTCAGAGGTTACATCAGCTGAAGTTCATATGTTATGATCCCTTGTCGTTTCTAAAGACCCAATTACATGTTCTGAAAAGGAACTGCAAAGTTCTTCCTTCATTGTCTCAAAAGAGGTTGTCCAATAATGTGATGAACTGCTATAGAGTTCTTGTCACCCCATCAAGAATTTATTGTTTGGGACCTGAGCTTGAAACTTCTAATTATGTTGTGAAAAACTTTGCATCATATGCTTCTGATTTTATGAGGGTTACTTTTGTTGAAGAGGACTGGAGTAAGCTTCCTGCAGGTGCCGTTACACGAGTATACAGCGTGGCTTACTCTCGAAACCTTATAGAACTGAAATCTATCACCGAATAG

mRNA sequence

ATGGCTTCCAAATTCAGCAGCAGTCGCTACCGTCTATGCAAAGAAGAACACGATTATCTTTGGATTCGGACAACAGAGTTTTCTTCTCTGAGATCAATAGGTCAGTCCACTTCCTTTTGTTGGGAAGTTGAAGAAGATTTGCAGGCTTCAGATATCTTTTCTAGTTTCCCACTTTACAGGGAAACTCCAAAAGATATTACATTAGAGGATGGTGGAGAATTTGGCTCTACCTCAGAGGCTGTTCCTCTGATAAAATCTGGGTTAGTTTCTAATCTAGCTTATGAGGTTCAATATCAGCTCAATTCCTTAGTTCATATGCAGAAAATTAGCCTTGCTGCAGCCAGCAATGATCTGATTGAATTCCTTAGCACTTTAGGTACTGACACTGCGTTGAATATACTTCAGAGGTTACATCAGCTGAAGTTCATATGTTATGATCCCTTGTCGTTTCTAAAGACCCAATTACATGTTCTGAAAAGGAACTGCAAAGTTCTTCCTTCATTGTCTCAAAAGAGGTTGTCCAATAATGTGATGAACTGCTATAGAGTTCTTGTCACCCCATCAAGAATTTATTGTTTGGGACCTGAGCTTGAAACTTCTAATTATGTTGTGAAAAACTTTGCATCATATGCTTCTGATTTTATGAGGGTTACTTTTGTTGAAGAGGACTGGAGTAAGCTTCCTGCAGGTGCCGTTACACGAGTATACAGCGTGGCTTACTCTCGAAACCTTATAGAACTGAAATCTATCACCGAATAG

Coding sequence (CDS)

ATGGCTTCCAAATTCAGCAGCAGTCGCTACCGTCTATGCAAAGAAGAACACGATTATCTTTGGATTCGGACAACAGAGTTTTCTTCTCTGAGATCAATAGGTCAGTCCACTTCCTTTTGTTGGGAAGTTGAAGAAGATTTGCAGGCTTCAGATATCTTTTCTAGTTTCCCACTTTACAGGGAAACTCCAAAAGATATTACATTAGAGGATGGTGGAGAATTTGGCTCTACCTCAGAGGCTGTTCCTCTGATAAAATCTGGGTTAGTTTCTAATCTAGCTTATGAGGTTCAATATCAGCTCAATTCCTTAGTTCATATGCAGAAAATTAGCCTTGCTGCAGCCAGCAATGATCTGATTGAATTCCTTAGCACTTTAGGTACTGACACTGCGTTGAATATACTTCAGAGGTTACATCAGCTGAAGTTCATATGTTATGATCCCTTGTCGTTTCTAAAGACCCAATTACATGTTCTGAAAAGGAACTGCAAAGTTCTTCCTTCATTGTCTCAAAAGAGGTTGTCCAATAATGTGATGAACTGCTATAGAGTTCTTGTCACCCCATCAAGAATTTATTGTTTGGGACCTGAGCTTGAAACTTCTAATTATGTTGTGAAAAACTTTGCATCATATGCTTCTGATTTTATGAGGGTTACTTTTGTTGAAGAGGACTGGAGTAAGCTTCCTGCAGGTGCCGTTACACGAGTATACAGCGTGGCTTACTCTCGAAACCTTATAGAACTGAAATCTATCACCGAATAG

Protein sequence

MASKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYRETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLIEFLSTLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNCYRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVTRVYSVAYSRNLIELKSITE

Homology

[Close]

BLAST of Csor.00g238520 vs. ExPASy Swiss-Prot
Match: O82504 (RNA-dependent RNA polymerase 2 OS=Arabidopsis thaliana OX=3702 GN=RDR2 PE=1 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 3.8e-65
Identity = 128/236 (54.24%), Postives = 167/236 (70.76%), Query Frame = 0


Query: 1   MASKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYR 60
           +A+KF S RYR CKE+ D++WIRTT+FS  +SIG ST FC EV       DIFS  P YR
Sbjct: 199 IATKFKSDRYRFCKEDFDFMWIRTTDFSGSKSIGTSTCFCLEVHNGSTMLDIFSGLPYYR 258

Query: 61  ETPKDITLEDGGEFGSTSEAVPLIKSGLVS-NLAYEVQYQLNSLVHMQKISLAAASN-DL 120
           E    +T  DG  F S ++ VPL+ + ++     YE+ +QLN+LVH QKISL AAS+ +L
Sbjct: 259 EDTLSLTYVDGKTFASAAQIVPLLNAAILGLEFPYEILFQLNALVHAQKISLFAASDMEL 318

Query: 121 IEFLSTLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLS-NNV 180
           I+ L  +  +TAL IL++LHQ   ICYDP+ F+KTQ+  + +  K  P+ + KRL+  N+
Sbjct: 319 IKILRGMSLETALVILKKLHQQSSICYDPVFFVKTQMQSVVKKMKHSPASAYKRLTEQNI 378

Query: 181 MNCYRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT 234
           M+C R  VTPS+IY LGPELET+NYVVKNFA + SDFMRVTFVEEDWSKLPA A++
Sbjct: 379 MSCQRAYVTPSKIYLLGPELETANYVVKNFAEHVSDFMRVTFVEEDWSKLPANALS 434

[Close]

BLAST of Csor.00g238520 vs. ExPASy Swiss-Prot
Match: Q7XM31 (Probable RNA-dependent RNA polymerase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=RDR2 PE=2 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 6.5e-41
Identity = 87/232 (37.50%), Postives = 140/232 (60.34%), Query Frame = 0


Query: 3   SKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYRET 62
           S+F   R+  CKE+  + W+R  +F+   S G+ ++   ++ +    SDI  S P +   
Sbjct: 199 SRFMDDRFHACKEDAKFSWVRALDFTPNYSFGRCSTLVLKLGKSALVSDILKSLP-FSGN 258

Query: 63  PKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLIEFL 122
             ++T+      G++S  VPL+      ++ YEV ++LNSL+HM KI     + DL + L
Sbjct: 259 LGELTMNSMDGVGASSNVVPLVHCPRDYSVPYEVLFRLNSLMHMGKIVAKHVNADLFKAL 318

Query: 123 STLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPS-LSQKRLSNNVMNCY 182
             L  D +  I +++H+L+  CY PL F++ + + +KR+  VL S   +      +M CY
Sbjct: 319 QELPVDVSRRIFEKMHKLESTCYGPLQFIQQEAYSMKRSHNVLLSNEGEGEGERKLMKCY 378

Query: 183 RVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT 234
           RV +TPS+I+C GPE E +NYVVK+ ++YASDF+RVTFV+EDWSKL + A++
Sbjct: 379 RVNITPSKIFCFGPEEEVTNYVVKHHSAYASDFVRVTFVDEDWSKLSSNAIS 429

[Close]

BLAST of Csor.00g238520 vs. ExPASy Swiss-Prot
Match: Q9LQV2 (RNA-dependent RNA polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=RDR1 PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.9e-25
Identity = 73/216 (33.80%), Postives = 119/216 (55.09%), Query Frame = 0


Query: 18  DYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYRE-TPKDITLEDGGEFGS 77
           D  WIRTT+F+S   IGQST+FC E+   L   D   +F  Y E       +E G  + S
Sbjct: 202 DEQWIRTTDFTSSSCIGQSTAFCLELPVHLNVPDFRENFANYAEHRASSFLIESGSSYSS 261

Query: 78  TSEA-VPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLIEFLSTLGTDTAL--NI 137
            +   VP++      +L +E+ ++LN+LV    +S  A   D    L+    D AL  + 
Sbjct: 262 NANTLVPVVDPPPGFSLPFEILFKLNTLVQNACLSGPALDLDFYRLLNQKKYDRALIDHC 321

Query: 138 LQRLHQLKFICYDPLSFLKTQLH--VLKRNCKVLPSLSQKRLSNNVMNCYRVLVTPSRIY 197
           L++L  L   CY+P  +L+ +    + K    + P++S   L + ++  YRV VTP+R+Y
Sbjct: 322 LEKLFHLGECCYEPAHWLRDEYKKWISKGKLPLSPTIS---LDDGLVYMYRVQVTPARVY 381

Query: 198 CLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKL 228
             GPE+  SN V+++++ Y ++F+RV+FV+ED  K+
Sbjct: 382 FSGPEVNVSNRVLRHYSKYINNFLRVSFVDEDLEKV 414

[Close]

BLAST of Csor.00g238520 vs. ExPASy Swiss-Prot
Match: Q9SG02 (RNA-dependent RNA polymerase 6 OS=Arabidopsis thaliana OX=3702 GN=RDR6 PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 7.0e-11
Identity = 62/227 (27.31%), Postives = 107/227 (47.14%), Query Frame = 0


Query: 16  EHDYLWIRTTEFSSLRSIGQSTSF----CWEVEEDLQ-ASDIFSSFPLYRET---PKDIT 75
           + D  WIRTT+F+ + +IG+  S+        E  L+ A D F    +  E    P  I 
Sbjct: 245 DDDDPWIRTTDFTQVGAIGRCHSYRVLISPRYENKLRTALDYFRMRRVQEERVRWPPRIR 304

Query: 76  LED-GGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLIEFLSTLG 135
            E   GE  S        K G    +++E+ + +NS++H    +    +    + L    
Sbjct: 305 NEPCFGEPVSDHFFCIHHKEG----ISFEIMFLVNSVLHRGVFNQFQLTERFFDLLRNQP 364

Query: 136 TDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNCYRVLVT 195
            D  +  L+ L   K   +D    LK     +++N K+L S  Q   S ++    R+++T
Sbjct: 365 KDVNIASLKHLCTYKRPVFDAYKRLKLVQEWIQKNPKLLGSHEQ---SEDISEIRRLVIT 424

Query: 196 PSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT 234
           P+R YCL PE+E SN V++ + + A  F+RVTF++E    + +  ++
Sbjct: 425 PTRAYCLPPEVELSNRVLRRYKAVAERFLRVTFMDESMQTINSNVLS 464

[Close]

BLAST of Csor.00g238520 vs. ExPASy Swiss-Prot
Match: Q8LHH9 (Probable RNA-dependent RNA polymerase SHL2 OS=Oryza sativa subsp. japonica OX=39947 GN=SHL2 PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.7e-09
Identity = 45/159 (28.30%), Postives = 80/159 (50.31%), Query Frame = 0


Query: 64  KDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLIEFLS 123
           + +T+ D  EFG   + V          L + + + +N+LVH   I+    + +    L 
Sbjct: 325 RGLTVRDELEFGEPMQDVFFCLQH-AEGLKFPLLFMVNALVHKGIINQHQLTPEFFSLLG 384

Query: 124 TLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNCYRV 183
               +  +  L+     KF  +D    LK  L+ + RN K+L S    ++ ++     R+
Sbjct: 385 RSEENVNVAALRDFWGDKFPVFDACGRLKKALNRVARNPKLLCS----KVGDDHAEVRRL 444

Query: 184 LVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEE 223
           ++TP+R YCL PE+E SN V++++   A  F+RVTF++E
Sbjct: 445 VITPTRAYCLPPEVERSNRVLRHYHEVADRFLRVTFMDE 478

[Close]

BLAST of Csor.00g238520 vs. NCBI nr
Match: KAG6590317.1 (RNA-dependent RNA polymerase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 496 bits (1276), Expect = 1.09e-176
Identity = 252/252 (100.00%), Postives = 252/252 (100.00%), Query Frame = 0


Query: 1   MASKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYR 60
           MASKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYR
Sbjct: 1   MASKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYR 60

Query: 61  ETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLIE 120
           ETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLIE
Sbjct: 61  ETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLIE 120

Query: 121 FLSTLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNC 180
           FLSTLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNC
Sbjct: 121 FLSTLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNC 180

Query: 181 YRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVTRVYSVAY 240
           YRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVTRVYSVAY
Sbjct: 181 YRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVTRVYSVAY 240

Query: 241 SRNLIELKSITE 252
           SRNLIELKSITE
Sbjct: 241 SRNLIELKSITE 252

[Close]

BLAST of Csor.00g238520 vs. NCBI nr
Match: KAG6589458.1 (RNA-dependent RNA polymerase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023142.1 RNA-dependent RNA polymerase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 461 bits (1186), Expect = 1.16e-151
Identity = 233/233 (100.00%), Postives = 233/233 (100.00%), Query Frame = 0


Query: 1   MASKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYR 60
           MASKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYR
Sbjct: 194 MASKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYR 253

Query: 61  ETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLIE 120
           ETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLIE
Sbjct: 254 ETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLIE 313

Query: 121 FLSTLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNC 180
           FLSTLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNC
Sbjct: 314 FLSTLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNC 373

Query: 181 YRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT 233
           YRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT
Sbjct: 374 YRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT 426

[Close]

BLAST of Csor.00g238520 vs. NCBI nr
Match: XP_023516327.1 (RNA-dependent RNA polymerase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 460 bits (1183), Expect = 3.24e-151
Identity = 232/233 (99.57%), Postives = 233/233 (100.00%), Query Frame = 0


Query: 1   MASKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYR 60
           MASKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYR
Sbjct: 194 MASKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYR 253

Query: 61  ETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLIE 120
           ETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYE+QYQLNSLVHMQKISLAAASNDLIE
Sbjct: 254 ETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYELQYQLNSLVHMQKISLAAASNDLIE 313

Query: 121 FLSTLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNC 180
           FLSTLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNC
Sbjct: 314 FLSTLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNC 373

Query: 181 YRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT 233
           YRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT
Sbjct: 374 YRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT 426

[Close]

BLAST of Csor.00g238520 vs. NCBI nr
Match: XP_022988330.1 (RNA-dependent RNA polymerase 2 [Cucurbita maxima])

HSP 1 Score: 457 bits (1177), Expect = 2.51e-150
Identity = 231/233 (99.14%), Postives = 232/233 (99.57%), Query Frame = 0


Query: 1   MASKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYR 60
           MASKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYR
Sbjct: 194 MASKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYR 253

Query: 61  ETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLIE 120
           ETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAA+NDLIE
Sbjct: 254 ETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAANNDLIE 313

Query: 121 FLSTLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNC 180
           FLSTLGTDTALNILQRLHQLK ICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNC
Sbjct: 314 FLSTLGTDTALNILQRLHQLKLICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNC 373

Query: 181 YRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT 233
           YRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT
Sbjct: 374 YRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT 426

[Close]

BLAST of Csor.00g238520 vs. NCBI nr
Match: XP_022921679.1 (RNA-dependent RNA polymerase 2 [Cucurbita moschata])

HSP 1 Score: 456 bits (1174), Expect = 7.00e-150
Identity = 230/233 (98.71%), Postives = 232/233 (99.57%), Query Frame = 0


Query: 1   MASKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYR 60
           MA KFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASD+FSSFPLYR
Sbjct: 194 MAYKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDMFSSFPLYR 253

Query: 61  ETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLIE 120
           ETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLI+
Sbjct: 254 ETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLID 313

Query: 121 FLSTLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNC 180
           FLSTLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNC
Sbjct: 314 FLSTLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNC 373

Query: 181 YRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT 233
           YRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT
Sbjct: 374 YRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT 426

[Close]

BLAST of Csor.00g238520 vs. ExPASy TrEMBL
Match: A0A6J1JJ96 (RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111485606 PE=3 SV=1)

HSP 1 Score: 457 bits (1177), Expect = 1.22e-150
Identity = 231/233 (99.14%), Postives = 232/233 (99.57%), Query Frame = 0


Query: 1   MASKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYR 60
           MASKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYR
Sbjct: 194 MASKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYR 253

Query: 61  ETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLIE 120
           ETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAA+NDLIE
Sbjct: 254 ETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAANNDLIE 313

Query: 121 FLSTLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNC 180
           FLSTLGTDTALNILQRLHQLK ICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNC
Sbjct: 314 FLSTLGTDTALNILQRLHQLKLICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNC 373

Query: 181 YRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT 233
           YRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT
Sbjct: 374 YRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT 426

[Close]

BLAST of Csor.00g238520 vs. ExPASy TrEMBL
Match: A0A6J1E171 (RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111429858 PE=3 SV=1)

HSP 1 Score: 456 bits (1174), Expect = 3.39e-150
Identity = 230/233 (98.71%), Postives = 232/233 (99.57%), Query Frame = 0


Query: 1   MASKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYR 60
           MA KFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASD+FSSFPLYR
Sbjct: 194 MAYKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDMFSSFPLYR 253

Query: 61  ETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLIE 120
           ETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLI+
Sbjct: 254 ETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLID 313

Query: 121 FLSTLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNC 180
           FLSTLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNC
Sbjct: 314 FLSTLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNC 373

Query: 181 YRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT 233
           YRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT
Sbjct: 374 YRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT 426

[Close]

BLAST of Csor.00g238520 vs. ExPASy TrEMBL
Match: A0A6J1C343 (RNA-dependent RNA polymerase OS=Momordica charantia OX=3673 GN=LOC111007004 PE=3 SV=1)

HSP 1 Score: 412 bits (1059), Expect = 3.39e-133
Identity = 202/233 (86.70%), Postives = 220/233 (94.42%), Query Frame = 0


Query: 1   MASKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYR 60
           MA+KFSS+RYRLCKE++DYLW+RTTEFS++RSIGQSTSFCWE+EEDLQASD+FSSFP Y+
Sbjct: 194 MATKFSSTRYRLCKEDYDYLWVRTTEFSAMRSIGQSTSFCWEIEEDLQASDVFSSFPFYK 253

Query: 61  ETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLIE 120
           E  KD  LE+G EFGSTSE VPLIKSGL SNLAYEVQYQLNSLVHMQKISLAAA+NDL++
Sbjct: 254 ENLKDFVLEEGEEFGSTSEIVPLIKSGLGSNLAYEVQYQLNSLVHMQKISLAAANNDLVD 313

Query: 121 FLSTLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNC 180
           FLSTL  +TALNILQ+LHQLKFICY+PLSFLKTQLHVLKRNCK LPS SQKRLSNN+MNC
Sbjct: 314 FLSTLSVETALNILQKLHQLKFICYEPLSFLKTQLHVLKRNCKNLPSSSQKRLSNNLMNC 373

Query: 181 YRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT 233
           YR LVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT
Sbjct: 374 YRALVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT 426

[Close]

BLAST of Csor.00g238520 vs. ExPASy TrEMBL
Match: A0A1S3BVE2 (RNA-dependent RNA polymerase OS=Cucumis melo OX=3656 GN=LOC103493922 PE=3 SV=1)

HSP 1 Score: 406 bits (1044), Expect = 4.96e-131
Identity = 203/233 (87.12%), Postives = 215/233 (92.27%), Query Frame = 0


Query: 1   MASKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYR 60
           MASKFSS+RYRLC E++DYLW+RTTEFS +RS+GQSTSFCWEVEEDLQASDIFS FP Y+
Sbjct: 194 MASKFSSTRYRLCVEDYDYLWVRTTEFSPMRSVGQSTSFCWEVEEDLQASDIFSYFPFYK 253

Query: 61  ETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLIE 120
           ET KDI LEDG EF STSE VPLIKSGL SNLAYEV+YQLNSLVHMQKISLAAA+ DLI+
Sbjct: 254 ETQKDIVLEDGEEFCSTSEIVPLIKSGLGSNLAYEVEYQLNSLVHMQKISLAAANQDLID 313

Query: 121 FLSTLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNC 180
           FL TL  DTALN+LQRLHQLKFICYDPLSFLKTQLHV KRNCK LP  SQKRLSNNVMNC
Sbjct: 314 FLCTLDIDTALNVLQRLHQLKFICYDPLSFLKTQLHVFKRNCKSLPLSSQKRLSNNVMNC 373

Query: 181 YRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT 233
           YRVLVTPSRIYCLGPELETSNYVVKNF+SYASDFMRVTFVEEDWSKLPAGAVT
Sbjct: 374 YRVLVTPSRIYCLGPELETSNYVVKNFSSYASDFMRVTFVEEDWSKLPAGAVT 426

[Close]

BLAST of Csor.00g238520 vs. ExPASy TrEMBL
Match: A0A5D3BHS8 (RNA-dependent RNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001880 PE=3 SV=1)

HSP 1 Score: 406 bits (1044), Expect = 6.63e-131
Identity = 203/233 (87.12%), Postives = 215/233 (92.27%), Query Frame = 0


Query: 1   MASKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYR 60
           MASKFSS+RYRLC E++DYLW+RTTEFS +RS+GQSTSFCWEVEEDLQASDIFS FP Y+
Sbjct: 209 MASKFSSTRYRLCVEDYDYLWVRTTEFSPMRSVGQSTSFCWEVEEDLQASDIFSYFPFYK 268

Query: 61  ETPKDITLEDGGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLIE 120
           ET KDI LEDG EF STSE VPLIKSGL SNLAYEV+YQLNSLVHMQKISLAAA+ DLI+
Sbjct: 269 ETQKDIVLEDGEEFCSTSEIVPLIKSGLGSNLAYEVEYQLNSLVHMQKISLAAANQDLID 328

Query: 121 FLSTLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNC 180
           FL TL  DTALN+LQRLHQLKFICYDPLSFLKTQLHV KRNCK LP  SQKRLSNNVMNC
Sbjct: 329 FLCTLDIDTALNVLQRLHQLKFICYDPLSFLKTQLHVFKRNCKSLPLSSQKRLSNNVMNC 388

Query: 181 YRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT 233
           YRVLVTPSRIYCLGPELETSNYVVKNF+SYASDFMRVTFVEEDWSKLPAGAVT
Sbjct: 389 YRVLVTPSRIYCLGPELETSNYVVKNFSSYASDFMRVTFVEEDWSKLPAGAVT 441

[Close]

BLAST of Csor.00g238520 vs. TAIR 10
Match: AT4G11130.1 (RNA-dependent RNA polymerase 2 )

HSP 1 Score: 249.6 bits (636), Expect = 2.7e-66
Identity = 128/236 (54.24%), Postives = 167/236 (70.76%), Query Frame = 0


Query: 1   MASKFSSSRYRLCKEEHDYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYR 60
           +A+KF S RYR CKE+ D++WIRTT+FS  +SIG ST FC EV       DIFS  P YR
Sbjct: 199 IATKFKSDRYRFCKEDFDFMWIRTTDFSGSKSIGTSTCFCLEVHNGSTMLDIFSGLPYYR 258

Query: 61  ETPKDITLEDGGEFGSTSEAVPLIKSGLVS-NLAYEVQYQLNSLVHMQKISLAAASN-DL 120
           E    +T  DG  F S ++ VPL+ + ++     YE+ +QLN+LVH QKISL AAS+ +L
Sbjct: 259 EDTLSLTYVDGKTFASAAQIVPLLNAAILGLEFPYEILFQLNALVHAQKISLFAASDMEL 318

Query: 121 IEFLSTLGTDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLS-NNV 180
           I+ L  +  +TAL IL++LHQ   ICYDP+ F+KTQ+  + +  K  P+ + KRL+  N+
Sbjct: 319 IKILRGMSLETALVILKKLHQQSSICYDPVFFVKTQMQSVVKKMKHSPASAYKRLTEQNI 378

Query: 181 MNCYRVLVTPSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT 234
           M+C R  VTPS+IY LGPELET+NYVVKNFA + SDFMRVTFVEEDWSKLPA A++
Sbjct: 379 MSCQRAYVTPSKIYLLGPELETANYVVKNFAEHVSDFMRVTFVEEDWSKLPANALS 434

[Close]

BLAST of Csor.00g238520 vs. TAIR 10
Match: AT1G14790.1 (RNA-dependent RNA polymerase 1 )

HSP 1 Score: 117.1 bits (292), Expect = 2.1e-26
Identity = 73/216 (33.80%), Postives = 119/216 (55.09%), Query Frame = 0


Query: 18  DYLWIRTTEFSSLRSIGQSTSFCWEVEEDLQASDIFSSFPLYRE-TPKDITLEDGGEFGS 77
           D  WIRTT+F+S   IGQST+FC E+   L   D   +F  Y E       +E G  + S
Sbjct: 202 DEQWIRTTDFTSSSCIGQSTAFCLELPVHLNVPDFRENFANYAEHRASSFLIESGSSYSS 261

Query: 78  TSEA-VPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLIEFLSTLGTDTAL--NI 137
            +   VP++      +L +E+ ++LN+LV    +S  A   D    L+    D AL  + 
Sbjct: 262 NANTLVPVVDPPPGFSLPFEILFKLNTLVQNACLSGPALDLDFYRLLNQKKYDRALIDHC 321

Query: 138 LQRLHQLKFICYDPLSFLKTQLH--VLKRNCKVLPSLSQKRLSNNVMNCYRVLVTPSRIY 197
           L++L  L   CY+P  +L+ +    + K    + P++S   L + ++  YRV VTP+R+Y
Sbjct: 322 LEKLFHLGECCYEPAHWLRDEYKKWISKGKLPLSPTIS---LDDGLVYMYRVQVTPARVY 381

Query: 198 CLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKL 228
             GPE+  SN V+++++ Y ++F+RV+FV+ED  K+
Sbjct: 382 FSGPEVNVSNRVLRHYSKYINNFLRVSFVDEDLEKV 414

[Close]

BLAST of Csor.00g238520 vs. TAIR 10
Match: AT3G49500.1 (RNA-dependent RNA polymerase 6 )

HSP 1 Score: 69.3 bits (168), Expect = 4.9e-12
Identity = 62/227 (27.31%), Postives = 107/227 (47.14%), Query Frame = 0


Query: 16  EHDYLWIRTTEFSSLRSIGQSTSF----CWEVEEDLQ-ASDIFSSFPLYRET---PKDIT 75
           + D  WIRTT+F+ + +IG+  S+        E  L+ A D F    +  E    P  I 
Sbjct: 245 DDDDPWIRTTDFTQVGAIGRCHSYRVLISPRYENKLRTALDYFRMRRVQEERVRWPPRIR 304

Query: 76  LED-GGEFGSTSEAVPLIKSGLVSNLAYEVQYQLNSLVHMQKISLAAASNDLIEFLSTLG 135
            E   GE  S        K G    +++E+ + +NS++H    +    +    + L    
Sbjct: 305 NEPCFGEPVSDHFFCIHHKEG----ISFEIMFLVNSVLHRGVFNQFQLTERFFDLLRNQP 364

Query: 136 TDTALNILQRLHQLKFICYDPLSFLKTQLHVLKRNCKVLPSLSQKRLSNNVMNCYRVLVT 195
            D  +  L+ L   K   +D    LK     +++N K+L S  Q   S ++    R+++T
Sbjct: 365 KDVNIASLKHLCTYKRPVFDAYKRLKLVQEWIQKNPKLLGSHEQ---SEDISEIRRLVIT 424

Query: 196 PSRIYCLGPELETSNYVVKNFASYASDFMRVTFVEEDWSKLPAGAVT 234
           P+R YCL PE+E SN V++ + + A  F+RVTF++E    + +  ++
Sbjct: 425 PTRAYCLPPEVELSNRVLRRYKAVAERFLRVTFMDESMQTINSNVLS 464

The following BLAST results are available for this feature:

BLAST of Csor.00g238520 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82504	3.8e-65	54.24	RNA-dependent RNA polymerase 2 OS=Arabidopsis thaliana OX=3702 GN=RDR2 PE=1 SV=1	[more]
Q7XM31	6.5e-41	37.50	Probable RNA-dependent RNA polymerase 2 OS=Oryza sativa subsp. japonica OX=39947...	[more]
Q9LQV2	2.9e-25	33.80	RNA-dependent RNA polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=RDR1 PE=2 SV=1	[more]
Q9SG02	7.0e-11	27.31	RNA-dependent RNA polymerase 6 OS=Arabidopsis thaliana OX=3702 GN=RDR6 PE=1 SV=1	[more]
Q8LHH9	1.7e-09	28.30	Probable RNA-dependent RNA polymerase SHL2 OS=Oryza sativa subsp. japonica OX=39...	[more]

BLAST of Csor.00g238520 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6590317.1	1.09e-176	100.00	RNA-dependent RNA polymerase 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG6589458.1	1.16e-151	100.00	RNA-dependent RNA polymerase 2, partial [Cucurbita argyrosperma subsp. sororia] ...	[more]
XP_023516327.1	3.24e-151	99.57	RNA-dependent RNA polymerase 2 [Cucurbita pepo subsp. pepo]	[more]
XP_022988330.1	2.51e-150	99.14	RNA-dependent RNA polymerase 2 [Cucurbita maxima]	[more]
XP_022921679.1	7.00e-150	98.71	RNA-dependent RNA polymerase 2 [Cucurbita moschata]	[more]

BLAST of Csor.00g238520 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JJ96	1.22e-150	99.14	RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111485606 PE=3 SV...	[more]
A0A6J1E171	3.39e-150	98.71	RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111429858 PE=3 ...	[more]
A0A6J1C343	3.39e-133	86.70	RNA-dependent RNA polymerase OS=Momordica charantia OX=3673 GN=LOC111007004 PE=3...	[more]
A0A1S3BVE2	4.96e-131	87.12	RNA-dependent RNA polymerase OS=Cucumis melo OX=3656 GN=LOC103493922 PE=3 SV=1	[more]
A0A5D3BHS8	6.63e-131	87.12	RNA-dependent RNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]

BLAST of Csor.00g238520 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G11130.1	2.7e-66	54.24	RNA-dependent RNA polymerase 2	[more]
AT1G14790.1	2.1e-26	33.80	RNA-dependent RNA polymerase 1	[more]
AT3G49500.1	4.9e-12	27.31	RNA-dependent RNA polymerase 6	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007855	RNA-dependent RNA polymerase, eukaryotic-type	PFAM	PF05183	RdRP	coord: 185..228 e-value: 4.2E-7 score: 29.0
IPR007855	RNA-dependent RNA polymerase, eukaryotic-type	PANTHER	PTHR23079	RNA-DEPENDENT RNA POLYMERASE	coord: 2..234
None	No IPR available	PANTHER	PTHR23079:SF5	RNA-DEPENDENT RNA POLYMERASE 2	coord: 2..234

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g238520.m01	Csor.00g238520.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050832	defense response to fungus
biological_process	GO:0010495	long-distance posttranscriptional gene silencing
biological_process	GO:0070919	production of siRNA involved in gene silencing by small RNA
biological_process	GO:0030422	production of siRNA involved in RNA interference
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:0001172	transcription, RNA-templated
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0031380	nuclear RNA-directed RNA polymerase complex
cellular_component	GO:0005730	nucleolus
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0003968	RNA-directed 5'-3' RNA polymerase activity