Csor.00g238380 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g238380
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionPectin lyase-like superfamily protein
LocationCsor_Chr08: 8144001 .. 8145885 (+)
RNA-Seq ExpressionCsor.00g238380
SyntenyCsor.00g238380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTTCAATCGAGTTCCTGCTGTGATGCTTTGGATCACGGCCATTTTGGTGGCGGAGAACTTGAGCAATGTGCAAGGAAGATACCATTTTCACAAGGGACAAAAGAAACAGAACCCTGATGAACAGCCCTCTCTTCCATCCCCTGTTTATTCACCACCACCGGAAGACTCGGTTCCTTCAACTCCAGCTGACCCAGCTCCCTCAGTGCCATCTGATCCCTACCCTGATGATCCAGGAAACACCACTTCTGACTGTGTTTTTGATGTCACTAATTATGGGGCGGTTGGAGATGGTTGTGCTGATGACACTGCTGCCTTCAGGGCAGCATGGAAAGCAGCCTGTGCTGTTGAATCTGCCACTCTTTTAGTTCCCTCTGATCGTTGCTTTAAAATCACTTCAACTATCTTCTCAGGACCTTGCAAACCAGGACTTGTGTTTAAGGTCAGCCACATTTTTTTAGAATTTGAATCTCTTGTTGTTCGTGTTGAAGTTGGAACACTTATAAGTAAACCTGTTAATGCAACAAATCAGGTGGATGGAACCCTGATGCCACCGGACGGACCAGAGTCGTGGCCGAAGGCGGACAGTTTGAGGCAATGGCTCGTGTTTTACAGGCTGGATCAAATGACATTGACTGGAACTGGAACCATCGAAGGGAATGGCCAAAAATGGTGGGAGTTGCCATGCAAACCACATAGGGTACCTACATTGTCCTCTTAATAGCTCTTGAATTTCTCTGTTTATTTCAGTTTCAACTTCCTATCCAAGGCTTAATTGTCCTTTTTATTTTAGGGTCCAAATGGGTCGACTCTGCCAGGACCATGTGACAGCCCTGCGGTAAGAAGGAAACCCACCATTTTGCCCACCAATTCAACCAACTTATATGAATAAAATATGCTGACTGGTTCTGATATCTGGGGTTTTCAGTTAATAAGGTTCTTCATGAGCAGTAATTTGGCAGTGAGTGGGCTGAGAATTCAGAACAGTCCACAGTTCCATATGAAATTCGATGGGTGTGAAGGAGTTCTGATAGACAAGCTGTCAATATCTTCCCCAAAGCTCAGTCCCAACACTGACGGAATCCATATAGAGAATACCAAAGGAGTAGGAATATACAACTCCATGATAGGCAATGGTATGAAACTGAAATAATGGATTGTTCTTGTCCACTCATTTTTATCAACATCTTGGCTGATCTATGATTTGGAAACATAGGTGATGACTGCATTTCAATCGGGCCCGGCTGTGCCAACGTAGACATTGAAGGCATCACTTGTGGGCCGAGTCATGGAATCAGGTACAAACAACGTCGATTGCGATCTCCATTTATTTGTTTATCACTAGTCAACGCAGTGAATAATTCATAGTTGTATGTAGCAGCATTGGGAGTCTGGGAGTCCACAATTCACAGGCATGCGTTTCCAACATAACAGTGAGAAATGCAGTGATTAGGGAATCAGACAATGGAGTGAGGATAAAGACATGGCAAGGAGGGTCGGGTACAGTGAGCGACATCTTATTCGAGAACATACAGATGGAGAATGTTAGAAACTGCATCATAGTTGACCAGTACTACTGCTTGTCCAAGGATTGTCGTAACCAGACATCGGCTGTGTTTGTGAACCAAGTCTTGTACAAGAACATCAAGGGAACTTACGACGTTAGGAACACTCCAATTCACTTCGCGTGCAGCGACACAGTGGCCTGCACGAATATTACAATGTCTGAAGTAGAATTGCTACCACATGAGGGTGAATTGGTGGAAGATCCTTTCTGTTGGAATGCATTTGGGATTCAGGAGACTTTGACCATTCCTCCCATTGATTGCTTGCAAGAAGGAGCACCTCAGAATGTCGCAGAGACCTATGAATATAGCTGCTGA

mRNA sequence

ATGGAGTTCAATCGAGTTCCTGCTGTGATGCTTTGGATCACGGCCATTTTGGTGGCGGAGAACTTGAGCAATGTGCAAGGAAGATACCATTTTCACAAGGGACAAAAGAAACAGAACCCTGATGAACAGCCCTCTCTTCCATCCCCTGTTTATTCACCACCACCGGAAGACTCGGTTCCTTCAACTCCAGCTGACCCAGCTCCCTCAGTGCCATCTGATCCCTACCCTGATGATCCAGGAAACACCACTTCTGACTGTGTTTTTGATGTCACTAATTATGGGGCGGTTGGAGATGGTTGTGCTGATGACACTGCTGCCTTCAGGGCAGCATGGAAAGCAGCCTGTGCTGTTGAATCTGCCACTCTTTTAGTTCCCTCTGATCGTTGCTTTAAAATCACTTCAACTATCTTCTCAGGACCTTGCAAACCAGGACTTGTGTTTAAGGTGGATGGAACCCTGATGCCACCGGACGGACCAGAGTCGTGGCCGAAGGCGGACAGTTTGAGGCAATGGCTCGTGTTTTACAGGCTGGATCAAATGACATTGACTGGAACTGGAACCATCGAAGGGAATGGCCAAAAATGGTGGGAGTTGCCATGCAAACCACATAGGGGTCCAAATGGGTCGACTCTGCCAGGACCATGTGACAGCCCTGCGTTAATAAGGTTCTTCATGAGCAGTAATTTGGCAGTGAGTGGGCTGAGAATTCAGAACAGTCCACAGTTCCATATGAAATTCGATGGGTGTGAAGGAGTTCTGATAGACAAGCTGTCAATATCTTCCCCAAAGCTCAGTCCCAACACTGACGGAATCCATATAGAGAATACCAAAGGAGTAGGAATATACAACTCCATGATAGGCAATGTGAGAAATGCAGTGATTAGGGAATCAGACAATGGAGTGAGGATAAAGACATGGCAAGGAGGGTCGGGTACAGTGAGCGACATCTTATTCGAGAACATACAGATGGAGAATGTTAGAAACTGCATCATAGTTGACCAGTACTACTGCTTGTCCAAGGATTGTCGTAACCAGACATCGGCTGTGTTTGTGAACCAAGTCTTGTACAAGAACATCAAGGGAACTTACGACGTTAGGAACACTCCAATTCACTTCGCGTGCAGCGACACAGTGGCCTGCACGAATATTACAATGTCTGAAGTAGAATTGCTACCACATGAGGGTGAATTGGTGGAAGATCCTTTCTGTTGGAATGCATTTGGGATTCAGGAGACTTTGACCATTCCTCCCATTGATTGCTTGCAAGAAGGAGCACCTCAGAATGTCGCAGAGACCTATGAATATAGCTGCTGA

Coding sequence (CDS)

ATGGAGTTCAATCGAGTTCCTGCTGTGATGCTTTGGATCACGGCCATTTTGGTGGCGGAGAACTTGAGCAATGTGCAAGGAAGATACCATTTTCACAAGGGACAAAAGAAACAGAACCCTGATGAACAGCCCTCTCTTCCATCCCCTGTTTATTCACCACCACCGGAAGACTCGGTTCCTTCAACTCCAGCTGACCCAGCTCCCTCAGTGCCATCTGATCCCTACCCTGATGATCCAGGAAACACCACTTCTGACTGTGTTTTTGATGTCACTAATTATGGGGCGGTTGGAGATGGTTGTGCTGATGACACTGCTGCCTTCAGGGCAGCATGGAAAGCAGCCTGTGCTGTTGAATCTGCCACTCTTTTAGTTCCCTCTGATCGTTGCTTTAAAATCACTTCAACTATCTTCTCAGGACCTTGCAAACCAGGACTTGTGTTTAAGGTGGATGGAACCCTGATGCCACCGGACGGACCAGAGTCGTGGCCGAAGGCGGACAGTTTGAGGCAATGGCTCGTGTTTTACAGGCTGGATCAAATGACATTGACTGGAACTGGAACCATCGAAGGGAATGGCCAAAAATGGTGGGAGTTGCCATGCAAACCACATAGGGGTCCAAATGGGTCGACTCTGCCAGGACCATGTGACAGCCCTGCGTTAATAAGGTTCTTCATGAGCAGTAATTTGGCAGTGAGTGGGCTGAGAATTCAGAACAGTCCACAGTTCCATATGAAATTCGATGGGTGTGAAGGAGTTCTGATAGACAAGCTGTCAATATCTTCCCCAAAGCTCAGTCCCAACACTGACGGAATCCATATAGAGAATACCAAAGGAGTAGGAATATACAACTCCATGATAGGCAATGTGAGAAATGCAGTGATTAGGGAATCAGACAATGGAGTGAGGATAAAGACATGGCAAGGAGGGTCGGGTACAGTGAGCGACATCTTATTCGAGAACATACAGATGGAGAATGTTAGAAACTGCATCATAGTTGACCAGTACTACTGCTTGTCCAAGGATTGTCGTAACCAGACATCGGCTGTGTTTGTGAACCAAGTCTTGTACAAGAACATCAAGGGAACTTACGACGTTAGGAACACTCCAATTCACTTCGCGTGCAGCGACACAGTGGCCTGCACGAATATTACAATGTCTGAAGTAGAATTGCTACCACATGAGGGTGAATTGGTGGAAGATCCTTTCTGTTGGAATGCATTTGGGATTCAGGAGACTTTGACCATTCCTCCCATTGATTGCTTGCAAGAAGGAGCACCTCAGAATGTCGCAGAGACCTATGAATATAGCTGCTGA

Protein sequence

MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVPSTPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESATLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNVRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYSC
Homology
BLAST of Csor.00g238380 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 2.7e-167
Identity = 294/480 (61.25%), Postives = 350/480 (72.92%), Query Frame = 0

Query: 9   VMLWITAILVAENLSNV-QGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVPSTPADPA 68
           ++++I + ++  + +NV + RYH+HKG+          +  P+  PPP    P   A+P 
Sbjct: 8   LVIYIISTVLFGHFTNVARARYHYHKGRH--------GVTHPLPPPPPP---PLETANPP 67

Query: 69  PSVPSDPYPDD---PGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESATLLV 128
             VPSDPYP     PG++ S CVFDVT++GAVGDG  DDTAAF+ AWKAACAVES  +L 
Sbjct: 68  DQVPSDPYPSPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLA 127

Query: 129 PSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQMTLTG 188
           P    FKITSTIFSGPCKPGLVF++DG LMPPDGPE WP+ D+  QWLVFYRLD  T +G
Sbjct: 128 PEGGVFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSG 187

Query: 189 TGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHM 248
            GT+EGNGQKWW+LPCKPHRGP+GS+  GPC SP +IRFFMS+N+ V GLRIQNSPQFHM
Sbjct: 188 KGTVEGNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHM 247

Query: 249 KFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN---------------- 308
           KFDGC+GVLI+++ ISSPKLSPNTDGIH+ NT+ VGIYNS++ N                
Sbjct: 248 KFDGCQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDI 307

Query: 309 -----------------------------VRNAVIRESDNGVRIKTWQGGSGTVSDILFE 368
                                        VRN VIR+SDNG+R+KTWQGG+G+VS++LFE
Sbjct: 308 QGVTCGPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFE 367

Query: 369 NIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVA 428
           NIQMENV NCIIVDQYYC SKDCRN+TSAV V  V Y+NIKGTYDVR+ PIHFACSDTVA
Sbjct: 368 NIQMENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVA 427

Query: 429 CTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYE--YSC 438
           CTNITMSEVELLP EGELV+DPFCWNA+G QETLTIPPIDCL +G+P  V E Y+  Y C
Sbjct: 428 CTNITMSEVELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSPV-VEEAYDSNYGC 475

BLAST of Csor.00g238380 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 1.0e-65
Identity = 140/388 (36.08%), Postives = 200/388 (51.55%), Query Frame = 0

Query: 84  SDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESAT-LLVPSDRCFKITSTIFSGPCK 143
           S+ +  V N+GA G+G  DDT AF  AWK AC+ +  T +LVP +    +     SGPCK
Sbjct: 49  SERLLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCK 108

Query: 144 PGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKP 203
             L  ++ GT++ P+ P+ W +  + R+WL F+ L ++T+ G GT+ G GQ+WWE  CK 
Sbjct: 109 ARLTLQISGTIIAPNDPDVW-EGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCK- 168

Query: 204 HRGPNGSTLPGPC-DSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIDKLSISS 263
           H   N      PC  +P  + F    N+ V  L + +S Q H+    C  V I  L + +
Sbjct: 169 HNHSN------PCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIA 228

Query: 264 PKLSPNTDGIHIENTKGVGIYNSMIG---------------------------------- 323
           P  SPNTDGIHI  ++G+ I N+ +                                   
Sbjct: 229 PATSPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLG 288

Query: 324 -----------NVRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYY 383
                       V  A+I ++ NGVRIKTWQGGSG VS I+F NI+M NV N II+DQYY
Sbjct: 289 KSKSWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYY 348

Query: 384 CLS-KDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEG 424
           C S K C NQTSA+ +  + + +++GT       I  +CSD+  C NI + +++L P  G
Sbjct: 349 CDSRKPCANQTSAISIENISFVHVRGT-SASKEAIKISCSDSSPCRNILLQDIDLEPSNG 408

BLAST of Csor.00g238380 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.7e-65
Identity = 145/397 (36.52%), Postives = 205/397 (51.64%), Query Frame = 0

Query: 87  VFDVTNYGAVGDGCADDTAAFRAAWKAACAVESATLLVPSDRCFKITSTIFSGPCKPGLV 146
           +F+V  YGAVGDG  D T AF  AW+AAC   SA LLVP ++ F + +  F+GPC+P   
Sbjct: 58  IFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPSAMLLVPGNKKFVVNNLFFNGPCQPHFT 117

Query: 147 FKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGP 206
           FKVDG +     P SW    + R WL F +L   TL G G I+G G++WW   CK     
Sbjct: 118 FKVDGIIAAYQNPASW---KNNRIWLQFAKLTGFTLMGKGVIDGQGKQWWAGQCK---WV 177

Query: 207 NGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIDKLSISSPKLSP 266
           NG  +    D P  I+F  S+ L + GL++ NSP+FH+ F  CEGV I  +SI++P+ SP
Sbjct: 178 NGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPRDSP 237

Query: 267 NTDGIHIENTKGVGIYNSMIG--------------------------------------- 326
           NTDGI I  +K   +  + IG                                       
Sbjct: 238 NTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGRENSR 297

Query: 327 ------NVRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYCLSKD 386
                 +V  A   ++ NG+RIKTWQGGSG  S I++EN++M N  N I+++Q+YC S  
Sbjct: 298 AEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCTSAS 357

Query: 387 -CRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVED 436
            C+NQ SAV +  V YKNI+GT       I   CSD++ C +I +S++ L    G++   
Sbjct: 358 ACQNQRSAVQIQDVTYKNIRGT-SATAAAIQLKCSDSMPCKDIKLSDISLKLTSGKIAS- 417

BLAST of Csor.00g238380 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 1.0e-62
Identity = 139/350 (39.71%), Postives = 181/350 (51.71%), Query Frame = 0

Query: 87  VFDVTNYGAVGDGCADDTAAFRAAWKAACAVESATLLVPSDRCFKITSTIFSGPCKPGLV 146
           VF+V  YGAVGDG  D T AF   W AAC   SA LLVP+++ F + + +F GPC+P L 
Sbjct: 58  VFNVEQYGAVGDGKHDSTEAFATTWNAACKKASAVLLVPANKKFFVNNLVFRGPCQPHLS 117

Query: 147 FKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGP 206
           FKVDGT++    P  W  +   + WL F +L    L GTG I+G GQ+WW   CK     
Sbjct: 118 FKVDGTIVAQPDPARWKNS---KIWLQFAQLTDFNLMGTGVIDGQGQQWWAGQCKV---V 177

Query: 207 NGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIDKLSISSPKLSP 266
           NG T+    + P  I+   S ++ V  L + NSP+FH+ F  CEGV I  L I +P+ SP
Sbjct: 178 NGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIKAPRDSP 237

Query: 267 NTDGIHIENTKGVGIYNSMIG--------------------------------------- 326
           NTDGI I  +K   I   +IG                                       
Sbjct: 238 NTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISIGSLGRDNSR 297

Query: 327 ------NVRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYCLSKD 386
                 +V  A   ++ NG+RIKTWQGGSG  S I +EN++M N  N I+++Q+YC S  
Sbjct: 298 AEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTSAS 357

Query: 387 -CRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVEL 391
            C+NQ SAV +  V YKNI GT       I   CSD+V CT I +S V L
Sbjct: 358 ACQNQRSAVQIQGVTYKNIHGT-SATAAAIQLMCSDSVPCTGIQLSNVSL 400

BLAST of Csor.00g238380 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 2.8e-60
Identity = 137/357 (38.38%), Postives = 183/357 (51.26%), Query Frame = 0

Query: 87  VFDVTNYGAVGDGCADDTAAFRAAWKAACAVESATLLVPSDRCFKITSTIFSGPCKPGLV 146
           VF+V +YGAVGDG  D T AF   W AAC   SA  LVP+++ F + + +F GPC+P   
Sbjct: 59  VFNVEHYGAVGDGKHDSTDAFEKTWNAACNKLSAVFLVPANKKFVVNNLVFYGPCQPHFS 118

Query: 147 FKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGP 206
           FKVDGT+     P  W  +   + W+ F RL    L GTG I+G G +WW   CK     
Sbjct: 119 FKVDGTIAAYPDPAKWKNS---KIWMHFARLTDFNLMGTGVIDGQGNRWWSDQCKT---I 178

Query: 207 NGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIDKLSISSPKLSP 266
           NG T+      P  I+   S ++ V  L + NSP+FH+ F  C+GV I  + I +P+ SP
Sbjct: 179 NGRTVCNDKGRPTAIKIDFSKSVTVKELTLTNSPEFHLVFGECDGVKIQGIKIKAPRDSP 238

Query: 267 NTDGIHIENTK------------------GVGIYNSMI------------------GNVR 326
           NTDGI I  +K                  G G  N  I                  GN R
Sbjct: 239 NTDGIDIFASKRFEIEKCTIGTGDDCVAVGTGSSNITIKDLTCGPGHGMSIGSLGKGNSR 298

Query: 327 NAV---------IRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYCLS-K 386
           + V           ++ NG+RIKTWQGGSG  S I +EN++M N  N I+++Q+YC S  
Sbjct: 299 SEVSFVHLDGAKFIDTQNGLRIKTWQGGSGLASHITYENVEMINAENPILINQFYCTSAA 358

Query: 387 DCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGEL 398
            C+NQ SAV +  V +KNI GT       I   CSD+V C+NI +S V L    G++
Sbjct: 359 ACKNQRSAVKIQDVTFKNIHGT-SATTAAIQLMCSDSVPCSNIKLSNVFLKLTSGKV 408

BLAST of Csor.00g238380 vs. NCBI nr
Match: KAG6594032.1 (Polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 902 bits (2331), Expect = 0.0
Identity = 437/437 (100.00%), Postives = 437/437 (100.00%), Query Frame = 0

Query: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60
           MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60

Query: 61  STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120
           STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA
Sbjct: 61  STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120

Query: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180
           TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM
Sbjct: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180

Query: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240
           TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP
Sbjct: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240

Query: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNVRNAVIRESDNG 300
           QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNVRNAVIRESDNG
Sbjct: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNVRNAVIRESDNG 300

Query: 301 VRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIK 360
           VRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIK
Sbjct: 301 VRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIK 360

Query: 361 GTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDC 420
           GTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDC
Sbjct: 361 GTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDC 420

Query: 421 LQEGAPQNVAETYEYSC 437
           LQEGAPQNVAETYEYSC
Sbjct: 421 LQEGAPQNVAETYEYSC 437

BLAST of Csor.00g238380 vs. NCBI nr
Match: KAG7024752.1 (Polygalacturonase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 878 bits (2269), Expect = 0.0
Identity = 436/483 (90.27%), Postives = 436/483 (90.27%), Query Frame = 0

Query: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60
           MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60

Query: 61  STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120
           STPADPAP VPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA
Sbjct: 61  STPADPAPPVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120

Query: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180
           TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM
Sbjct: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180

Query: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240
           TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP
Sbjct: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240

Query: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN------------ 300
           QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN            
Sbjct: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPGCA 300

Query: 301 ----------------------------------VRNAVIRESDNGVRIKTWQGGSGTVS 360
                                             VRNAVIRESDNGVRIKTWQGGSGTVS
Sbjct: 301 NVDIEGVTCGPSHGISSIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVS 360

Query: 361 DILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFAC 420
           DILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFAC
Sbjct: 361 DILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFAC 420

Query: 421 SDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYE 437
           SDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYE
Sbjct: 421 SDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYE 480

BLAST of Csor.00g238380 vs. NCBI nr
Match: XP_022930280.1 (polygalacturonase At1g48100-like [Cucurbita moschata])

HSP 1 Score: 877 bits (2265), Expect = 0.0
Identity = 435/482 (90.25%), Postives = 436/482 (90.46%), Query Frame = 0

Query: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60
           MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60

Query: 61  STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120
           STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA
Sbjct: 61  STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120

Query: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180
           TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADS RQWLVFYRLDQM
Sbjct: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSSRQWLVFYRLDQM 180

Query: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240
           TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP
Sbjct: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240

Query: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN------------ 300
           QFHMKFDGCEGVLI+KLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN            
Sbjct: 241 QFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPGCA 300

Query: 301 ---------------------------------VRNAVIRESDNGVRIKTWQGGSGTVSD 360
                                            VRNAVIRESDNGVRIKTWQGGSGTVSD
Sbjct: 301 NVDIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSD 360

Query: 361 ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACS 420
           ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACS
Sbjct: 361 ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACS 420

Query: 421 DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY 437
           DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY
Sbjct: 421 DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY 480

BLAST of Csor.00g238380 vs. NCBI nr
Match: XP_023000015.1 (polygalacturonase At1g48100-like [Cucurbita maxima])

HSP 1 Score: 875 bits (2262), Expect = 0.0
Identity = 434/482 (90.04%), Postives = 435/482 (90.25%), Query Frame = 0

Query: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60
           MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDS P
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSAP 60

Query: 61  STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120
           STPAD APSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA
Sbjct: 61  STPADQAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120

Query: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180
           TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM
Sbjct: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180

Query: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240
           TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP
Sbjct: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240

Query: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN------------ 300
           QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN            
Sbjct: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPGCA 300

Query: 301 ---------------------------------VRNAVIRESDNGVRIKTWQGGSGTVSD 360
                                            VRNAVIRESDNGVRIKTWQGGSGTVSD
Sbjct: 301 NVDIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSD 360

Query: 361 ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACS 420
           ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQ+LYKNIKGTYDVRNTPIHFACS
Sbjct: 361 ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQILYKNIKGTYDVRNTPIHFACS 420

Query: 421 DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY 437
           DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY
Sbjct: 421 DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY 480

BLAST of Csor.00g238380 vs. NCBI nr
Match: XP_023514293.1 (polygalacturonase At1g48100-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 874 bits (2258), Expect = 0.0
Identity = 433/482 (89.83%), Postives = 435/482 (90.25%), Query Frame = 0

Query: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60
           MEFNRVPAVMLW TAILVA+NLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPP+DSVP
Sbjct: 1   MEFNRVPAVMLWFTAILVADNLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPQDSVP 60

Query: 61  STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120
           STPA PAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA
Sbjct: 61  STPAHPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120

Query: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180
           TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM
Sbjct: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180

Query: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240
           TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP
Sbjct: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240

Query: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN------------ 300
           QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN            
Sbjct: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPGCA 300

Query: 301 ---------------------------------VRNAVIRESDNGVRIKTWQGGSGTVSD 360
                                            VRNAVIRESDNGVRIKTWQGGSGTVSD
Sbjct: 301 NVDIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSD 360

Query: 361 ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACS 420
           ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACS
Sbjct: 361 ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACS 420

Query: 421 DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY 437
           DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY
Sbjct: 421 DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY 480

BLAST of Csor.00g238380 vs. ExPASy TrEMBL
Match: A0A6J1EWI8 (polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111436782 PE=3 SV=1)

HSP 1 Score: 877 bits (2265), Expect = 0.0
Identity = 435/482 (90.25%), Postives = 436/482 (90.46%), Query Frame = 0

Query: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60
           MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60

Query: 61  STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120
           STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA
Sbjct: 61  STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120

Query: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180
           TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADS RQWLVFYRLDQM
Sbjct: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSSRQWLVFYRLDQM 180

Query: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240
           TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP
Sbjct: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240

Query: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN------------ 300
           QFHMKFDGCEGVLI+KLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN            
Sbjct: 241 QFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPGCA 300

Query: 301 ---------------------------------VRNAVIRESDNGVRIKTWQGGSGTVSD 360
                                            VRNAVIRESDNGVRIKTWQGGSGTVSD
Sbjct: 301 NVDIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSD 360

Query: 361 ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACS 420
           ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACS
Sbjct: 361 ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACS 420

Query: 421 DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY 437
           DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY
Sbjct: 421 DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY 480

BLAST of Csor.00g238380 vs. ExPASy TrEMBL
Match: A0A6J1KH63 (polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111494324 PE=3 SV=1)

HSP 1 Score: 875 bits (2262), Expect = 0.0
Identity = 434/482 (90.04%), Postives = 435/482 (90.25%), Query Frame = 0

Query: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60
           MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDS P
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSAP 60

Query: 61  STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120
           STPAD APSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA
Sbjct: 61  STPADQAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120

Query: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180
           TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM
Sbjct: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180

Query: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240
           TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP
Sbjct: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240

Query: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN------------ 300
           QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN            
Sbjct: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPGCA 300

Query: 301 ---------------------------------VRNAVIRESDNGVRIKTWQGGSGTVSD 360
                                            VRNAVIRESDNGVRIKTWQGGSGTVSD
Sbjct: 301 NVDIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSD 360

Query: 361 ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACS 420
           ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQ+LYKNIKGTYDVRNTPIHFACS
Sbjct: 361 ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQILYKNIKGTYDVRNTPIHFACS 420

Query: 421 DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY 437
           DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY
Sbjct: 421 DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY 480

BLAST of Csor.00g238380 vs. ExPASy TrEMBL
Match: A0A6J1CB48 (polygalacturonase At1g48100 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111009879 PE=3 SV=1)

HSP 1 Score: 806 bits (2081), Expect = 1.34e-292
Identity = 400/490 (81.63%), Postives = 421/490 (85.92%), Query Frame = 0

Query: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60
           MEFNRV A+++ ITAIL+ EN SNVQGRYHFHKGQKKQ PDE PS+PSPVYSPPPE++VP
Sbjct: 1   MEFNRVLAILVCITAILLVENSSNVQGRYHFHKGQKKQKPDEDPSVPSPVYSPPPEEAVP 60

Query: 61  STPADPAPS--------VPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWK 120
           S+PA+PAPS        VPSDPYPDDP N+TSDCVFDVT+YGAVGDGC DDTAAFRAAWK
Sbjct: 61  SSPAEPAPSPPAEPAPSVPSDPYPDDPRNSTSDCVFDVTDYGAVGDGCTDDTAAFRAAWK 120

Query: 121 AACAVESATLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWL 180
           AACAVESAT+LVPSD CF ITSTIFSGPCKPGLVFKVDG+LMPPDGPESWPKADS+RQWL
Sbjct: 121 AACAVESATILVPSDHCFIITSTIFSGPCKPGLVFKVDGSLMPPDGPESWPKADSVRQWL 180

Query: 181 VFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVS 240
           VFYRLDQMT+TGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNL VS
Sbjct: 181 VFYRLDQMTMTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLVVS 240

Query: 241 GLRIQNSPQFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN---- 300
           GLRIQNSPQFHMKFDGCEGVLI+KLSISSPKLSPNTDGIHIENTKGVGIYNSMI N    
Sbjct: 241 GLRIQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDC 300

Query: 301 -----------------------------------------VRNAVIRESDNGVRIKTWQ 360
                                                    VRNAVIR+SDNGVRIKTWQ
Sbjct: 301 ISIGPGCANVDIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGVRIKTWQ 360

Query: 361 GGSGTVSDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRN 420
           GGSG+VSDILFENIQMENVRNCIIVDQYYCLSK C NQTSAVFVNQVLY+NIKGTYDVRN
Sbjct: 361 GGSGSVSDILFENIQMENVRNCIIVDQYYCLSKGCLNQTSAVFVNQVLYRNIKGTYDVRN 420

Query: 421 TPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQ 437
           TPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNA+GIQETLTIPPIDCLQ+G PQ
Sbjct: 421 TPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAYGIQETLTIPPIDCLQDGQPQ 480

BLAST of Csor.00g238380 vs. ExPASy TrEMBL
Match: A0A6J1CE60 (polygalacturonase At1g48100 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009879 PE=3 SV=1)

HSP 1 Score: 791 bits (2043), Expect = 2.25e-286
Identity = 400/517 (77.37%), Postives = 421/517 (81.43%), Query Frame = 0

Query: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60
           MEFNRV A+++ ITAIL+ EN SNVQGRYHFHKGQKKQ PDE PS+PSPVYSPPPE++VP
Sbjct: 1   MEFNRVLAILVCITAILLVENSSNVQGRYHFHKGQKKQKPDEDPSVPSPVYSPPPEEAVP 60

Query: 61  STPADPAPS--------VPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWK 120
           S+PA+PAPS        VPSDPYPDDP N+TSDCVFDVT+YGAVGDGC DDTAAFRAAWK
Sbjct: 61  SSPAEPAPSPPAEPAPSVPSDPYPDDPRNSTSDCVFDVTDYGAVGDGCTDDTAAFRAAWK 120

Query: 121 AACAVESATLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWL 180
           AACAVESAT+LVPSD CF ITSTIFSGPCKPGLVFKVDG+LMPPDGPESWPKADS+RQWL
Sbjct: 121 AACAVESATILVPSDHCFIITSTIFSGPCKPGLVFKVDGSLMPPDGPESWPKADSVRQWL 180

Query: 181 VFYRLDQMTLTGTGTIEGNGQKWWELPCKPHR---------------------------G 240
           VFYRLDQMT+TGTGTIEGNGQKWWELPCKPHR                           G
Sbjct: 181 VFYRLDQMTMTGTGTIEGNGQKWWELPCKPHRVGYPHYIYPLHYHSFSFNQRLNYFSLQG 240

Query: 241 PNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIDKLSISSPKLS 300
           PNGSTLPGPCDSPALIRFFMSSNL VSGLRIQNSPQFHMKFDGCEGVLI+KLSISSPKLS
Sbjct: 241 PNGSTLPGPCDSPALIRFFMSSNLVVSGLRIQNSPQFHMKFDGCEGVLIEKLSISSPKLS 300

Query: 301 PNTDGIHIENTKGVGIYNSMIGN------------------------------------- 360
           PNTDGIHIENTKGVGIYNSMI N                                     
Sbjct: 301 PNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVDIEGVTCGPSHGISIGSLGVHNS 360

Query: 361 --------VRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYCLSK 420
                   VRNAVIR+SDNGVRIKTWQGGSG+VSDILFENIQMENVRNCIIVDQYYCLSK
Sbjct: 361 QACVSNITVRNAVIRDSDNGVRIKTWQGGSGSVSDILFENIQMENVRNCIIVDQYYCLSK 420

Query: 421 DCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVED 437
            C NQTSAVFVNQVLY+NIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVED
Sbjct: 421 GCLNQTSAVFVNQVLYRNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVED 480

BLAST of Csor.00g238380 vs. ExPASy TrEMBL
Match: A0A0A0KB54 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G000120 PE=3 SV=1)

HSP 1 Score: 771 bits (1990), Expect = 2.95e-279
Identity = 386/467 (82.66%), Postives = 405/467 (86.72%), Query Frame = 0

Query: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60
           ME N V  VM+W+ AIL  EN SNV GRYHFHKG+K    DE  S+ SP     PE++VP
Sbjct: 1   MELNGVFIVMIWVVAILFVENSSNVNGRYHFHKGKK----DEDSSVSSP-----PENAVP 60

Query: 61  STPADPAPSVPSDPYPDDPG--NTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVE 120
           STPA P PSVPSDPYP+DPG  N+TSDCVFDVT++GAVGDGC DDTAAF+AAWKAACAVE
Sbjct: 61  STPAQPLPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVE 120

Query: 121 SATLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLD 180
           SATLLVPS+ CFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADS RQWLVFYRLD
Sbjct: 121 SATLLVPSNLCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLD 180

Query: 181 QMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQN 240
           QMTLTG+GTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAV+ LRIQN
Sbjct: 181 QMTLTGSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQN 240

Query: 241 SPQFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN---------- 300
           SP FHMKFDGCEGVLI+KLSISSPKLSPNTDGIHIENTKGVGIYNSMI N          
Sbjct: 241 SPMFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGVTCGPSHGI 300

Query: 301 ------------------VRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCI 360
                             VRNAVIR+SDNG+RIKTWQGGSG+VSDILFENIQMENVRNCI
Sbjct: 301 SIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVRNCI 360

Query: 361 IVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVEL 420
           IVDQYYCLSKDC NQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSD+VACTNITMSEVEL
Sbjct: 361 IVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSEVEL 420

Query: 421 LPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYSC 437
           LPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEG PQNVAET EYSC
Sbjct: 421 LPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 458

BLAST of Csor.00g238380 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 589.7 bits (1519), Expect = 1.9e-168
Identity = 294/480 (61.25%), Postives = 350/480 (72.92%), Query Frame = 0

Query: 9   VMLWITAILVAENLSNV-QGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVPSTPADPA 68
           ++++I + ++  + +NV + RYH+HKG+          +  P+  PPP    P   A+P 
Sbjct: 8   LVIYIISTVLFGHFTNVARARYHYHKGRH--------GVTHPLPPPPPP---PLETANPP 67

Query: 69  PSVPSDPYPDD---PGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESATLLV 128
             VPSDPYP     PG++ S CVFDVT++GAVGDG  DDTAAF+ AWKAACAVES  +L 
Sbjct: 68  DQVPSDPYPSPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLA 127

Query: 129 PSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQMTLTG 188
           P    FKITSTIFSGPCKPGLVF++DG LMPPDGPE WP+ D+  QWLVFYRLD  T +G
Sbjct: 128 PEGGVFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSG 187

Query: 189 TGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHM 248
            GT+EGNGQKWW+LPCKPHRGP+GS+  GPC SP +IRFFMS+N+ V GLRIQNSPQFHM
Sbjct: 188 KGTVEGNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHM 247

Query: 249 KFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN---------------- 308
           KFDGC+GVLI+++ ISSPKLSPNTDGIH+ NT+ VGIYNS++ N                
Sbjct: 248 KFDGCQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDI 307

Query: 309 -----------------------------VRNAVIRESDNGVRIKTWQGGSGTVSDILFE 368
                                        VRN VIR+SDNG+R+KTWQGG+G+VS++LFE
Sbjct: 308 QGVTCGPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFE 367

Query: 369 NIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVA 428
           NIQMENV NCIIVDQYYC SKDCRN+TSAV V  V Y+NIKGTYDVR+ PIHFACSDTVA
Sbjct: 368 NIQMENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVA 427

Query: 429 CTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYE--YSC 438
           CTNITMSEVELLP EGELV+DPFCWNA+G QETLTIPPIDCL +G+P  V E Y+  Y C
Sbjct: 428 CTNITMSEVELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSPV-VEEAYDSNYGC 475

BLAST of Csor.00g238380 vs. TAIR 10
Match: AT1G02460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 466.1 bits (1198), Expect = 3.1e-131
Identity = 240/481 (49.90%), Postives = 309/481 (64.24%), Query Frame = 0

Query: 9   VMLWITAILVAENLSNVQGRYH--FHKGQKKQNPDEQPSLPSPVYSPPPEDSVPSTPADP 68
           V+ ++T IL   +LS  +  +H   HK     +   +P  PS   S PP    P  P  P
Sbjct: 11  VLGFMTLILTLMSLSEARNHHHKEKHKHNNHNHHSSKPKPPSSSISQPPTPP-PGPPDSP 70

Query: 69  APSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESAT---LL 128
           APS+P  P  DDP +  +  +++V  YGAVGDG  DDT AF+ AW ++C  E+ T   LL
Sbjct: 71  APSLPPSP-SDDPADDNNG-IYNVRKYGAVGDGETDDTEAFKTAWDSSCNNENNTDSVLL 130

Query: 129 VPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQMTLT 188
           VP    F I STIF+GPC+    F+VDGT++ PDGPESWP   S RQWLVFYR++ M L 
Sbjct: 131 VPYGYTFMIQSTIFTGPCRSYQFFQVDGTIVTPDGPESWPSNISKRQWLVFYRVNGMALK 190

Query: 189 GTGTIEGNGQKWWELPCKPHRGPNGSTL-PGPCDSPALIRFFMSSNLAVSGLRIQNSPQF 248
           G G I+G GQKWW+LPCKPHR  N S +  GPCDSP  +RFFMSSNL V GL+I+NSPQF
Sbjct: 191 GEGVIDGRGQKWWDLPCKPHRSVNKSAIVTGPCDSPIALRFFMSSNLRVEGLQIKNSPQF 250

Query: 249 HMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN-------------- 308
           H +FDGC+GV ++ L I++P LSPNTDGIHIEN+  V IYNS+I N              
Sbjct: 251 HFRFDGCQGVHVESLHITAPPLSPNTDGIHIENSNSVTIYNSIISNGDDCVSIGSGSYDV 310

Query: 309 --------------------------------VRNAVIRESDNGVRIKTWQGGSGTVSDI 368
                                           VR++VI+ SDNGVRIKTWQGGSG+VS +
Sbjct: 311 DIRNLTCGPGGHGISIGSLGNHNSRACVSNITVRDSVIKYSDNGVRIKTWQGGSGSVSGV 370

Query: 369 LFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSD 428
            F NI +++VRN II+DQYYC++KDC N+TSAVFV+ + Y+ IKGTYD+R+ P+HF CSD
Sbjct: 371 TFNNIHVDSVRNPIIIDQYYCMTKDCANKTSAVFVSDIAYQGIKGTYDIRSPPMHFGCSD 430

Query: 429 TVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYS 438
            V CTN+T+S++ELLP +GE+V DPFCWNA+GI E L+IPP+ CL    P+ +  +    
Sbjct: 431 AVPCTNLTLSDIELLPAKGEIVLDPFCWNAYGIAEELSIPPVWCLMSDPPKGLQGSLVDK 488

BLAST of Csor.00g238380 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 411.4 bits (1056), Expect = 9.2e-115
Identity = 200/409 (48.90%), Postives = 266/409 (65.04%), Query Frame = 0

Query: 66  PAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAAC---AVESATL 125
           P+P+ P+  Y D+  +  +  VFDVT++GA+GD   DDT+AF+ AW AAC     +SA L
Sbjct: 29  PSPA-PNPAYNDN--DNIAPTVFDVTSFGAIGDCSTDDTSAFKMAWDAACMSTGPKSALL 88

Query: 126 LVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQMTL 185
           LVP   CF +  T F+GPC+  LV ++DG ++ PDGP SWP ++  RQW++FYR++ +++
Sbjct: 89  LVPYTFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWP-SNYQRQWMMFYRVNGLSI 148

Query: 186 TGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSPQF 245
            G+G I G GQKWW LPCKPH+G NG+T  GPCDSP  IR F SS + + G+   NS QF
Sbjct: 149 QGSGVINGRGQKWWNLPCKPHKGLNGTTQTGPCDSPVAIRLFQSSKVRIQGINFMNSAQF 208

Query: 246 HMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN-------------- 305
           H++FD C  V++D + I +P  SPNTDGIHIENT  V I NSMI N              
Sbjct: 209 HVRFDNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISNGDDCISIGAGCFNV 268

Query: 306 -------------------------------VRNAVIRESDNGVRIKTWQGGSGTVSDIL 365
                                          V N+ I  SDNGVRIKTWQGGSG+VS I+
Sbjct: 269 DIKNVTCGPSHGISIGSLGVHNSQAYVSNITVTNSTIWNSDNGVRIKTWQGGSGSVSRIV 328

Query: 366 FENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDT 425
           F NI M NVRN I++DQYYC + +C NQTSAV ++ VLY NIKGTYD+R+ PIHF CSD+
Sbjct: 329 FSNILMVNVRNPIMIDQYYCQTNNCANQTSAVIISDVLYANIKGTYDLRSPPIHFGCSDS 388

Query: 426 VACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAP 427
           V CTN+T++EV+L P +G+ +E+PFCWNA+G  + +T+PP+ CL +  P
Sbjct: 389 VPCTNLTLTEVDLFPSKGQHLENPFCWNAYGSMK-ITVPPVYCLLDAPP 432

BLAST of Csor.00g238380 vs. TAIR 10
Match: AT4G01890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 409.5 bits (1051), Expect = 3.5e-114
Identity = 203/415 (48.92%), Postives = 269/415 (64.82%), Query Frame = 0

Query: 61  STPADPAPSVPSDP--YPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVE 120
           S P  P+P+ P+DP   P    +   + V+DV  YGAVG+G ADDT +F+ AW +AC+  
Sbjct: 37  SQPPSPSPN-PNDPSKSPSRSQDLDHEVVYDVRKYGAVGNGVADDTVSFKTAWDSACSNN 96

Query: 121 ----SATLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVF 180
               ++ L VP    F I STIF+GPC+    F+VDGT++P DGP+SWP + + RQWL F
Sbjct: 97  KNNTASVLHVPYGFTFMIRSTIFTGPCRSYQYFQVDGTIVPRDGPKSWPSSLNKRQWLAF 156

Query: 181 YRLDQMTLTGTGTIEGNGQKWWELPCKPH-RGPNGSTLPGPCDSPALIRFFMSSNLAVSG 240
           YR++ M L G G I+G GQ WW+LPCKPH +  N + L GPC+SPA +RFFMSSN+ V G
Sbjct: 157 YRINGMALQGAGVIDGRGQNWWDLPCKPHQQNVNKTKLAGPCESPAALRFFMSSNVIVKG 216

Query: 241 LRIQNSPQFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN----- 300
           L I+NSPQ H+K DGC  V I+ L I SP  SPNTDGIHIEN+  V IYNS+I N     
Sbjct: 217 LSIKNSPQVHLKLDGCHVVHINSLRIISPPASPNTDGIHIENSNSVEIYNSVISNGDDCV 276

Query: 301 -----------------------------------------VRNAVIRESDNGVRIKTWQ 360
                                                    VR++ I+ S+NGVRIKTWQ
Sbjct: 277 SIGPGAYDIDIRNITCGPGGHGISIGSLGEKNSHACVSNVTVRDSFIKFSENGVRIKTWQ 336

Query: 361 GGSGTVSDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRN 420
           GGSG+VS + F+NI ++ VRN II+DQYYC +K C N+TSAVFVN ++Y++IKGTYD+R+
Sbjct: 337 GGSGSVSGVTFDNIHVDTVRNPIIIDQYYCTTKSCANKTSAVFVNDIVYQSIKGTYDIRS 396

Query: 421 TPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQ 423
            P+HF CS+ V CTN+T+S +ELLP + ++V  PFCWNA+GI +  ++P I CL+
Sbjct: 397 PPMHFGCSNNVPCTNLTLSNIELLPSKEDIVVGPFCWNAYGITDEFSVPLISCLK 450

BLAST of Csor.00g238380 vs. TAIR 10
Match: AT5G14650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 288.9 bits (738), Expect = 6.9e-78
Identity = 156/402 (38.81%), Postives = 218/402 (54.23%), Query Frame = 0

Query: 84  SDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESATLLVPSDRCFKITSTIFSG-PCK 143
           S    +V ++GA GDG +DDT AF  AW+ AC V ++TLLVPS   F +    F G  CK
Sbjct: 47  SSGTINVLDHGAKGDGTSDDTKAFEDAWQVACKVAASTLLVPSGSTFLVGPVSFLGKECK 106

Query: 144 PGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKP 203
             +VF+++G ++ P    +W     L QW+ F  L  +T+ G G I+G G  WW      
Sbjct: 107 EKIVFQLEGKIIAPTSASAW--GSGLLQWIEFKALQGITIKGKGIIDGRGSVWW------ 166

Query: 204 HRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIDKLSISSP 263
                G+ +P     P  +RF+ S+ + VSG+ IQNSPQ H+KFD C  + +   + SSP
Sbjct: 167 -NDMMGTKMPR--TKPTALRFYGSNGVTVSGITIQNSPQTHLKFDNCISIQVSDFTTSSP 226

Query: 264 KLSPNTDGIHIENTKGVGIYNSMIG----------------------------------- 323
             SPNTDGIH++N++   IY S +                                    
Sbjct: 227 GDSPNTDGIHLQNSQDAVIYRSTLACGDDCISIQTGCSNINIHDVDCGPGHGISIGGLGK 286

Query: 324 ----------NVRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYC 383
                      VR+  + E+ NGVRIK+WQGGSG+V  ++F NIQ+ NV N II+DQYYC
Sbjct: 287 DNTKACVSNITVRDVTMHETTNGVRIKSWQGGSGSVKQVMFSNIQVSNVANPIIIDQYYC 346

Query: 384 LSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGEL 438
               C N+TSAV V+ + Y NIKGTY     P+ FACSD++ CT I++S +EL P  G+ 
Sbjct: 347 DGGGCHNETSAVAVSNINYINIKGTY--TKEPVRFACSDSLPCTGISLSTIELKPATGKA 406

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949Z12.7e-16761.25Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV... [more]
Q94AJ51.0e-6536.08Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
P432121.7e-6536.52Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
Q7M1E71.0e-6239.71Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
Q9FY192.8e-6038.38Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6594032.10.0100.00Polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7024752.10.090.27Polygalacturonase [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022930280.10.090.25polygalacturonase At1g48100-like [Cucurbita moschata][more]
XP_023000015.10.090.04polygalacturonase At1g48100-like [Cucurbita maxima][more]
XP_023514293.10.089.83polygalacturonase At1g48100-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1EWI80.090.25polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111436782 P... [more]
A0A6J1KH630.090.04polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111494324 PE=... [more]
A0A6J1CB481.34e-29281.63polygalacturonase At1g48100 isoform X2 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1CE602.25e-28677.37polygalacturonase At1g48100 isoform X1 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A0A0KB542.95e-27982.66Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G000120 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G48100.11.9e-16861.25Pectin lyase-like superfamily protein [more]
AT1G02460.13.1e-13149.90Pectin lyase-like superfamily protein [more]
AT1G56710.19.2e-11548.90Pectin lyase-like superfamily protein [more]
AT4G01890.13.5e-11448.92Pectin lyase-like superfamily protein [more]
AT5G14650.16.9e-7838.81Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 80..434
e-value: 4.1E-88
score: 298.1
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 288..390
e-value: 2.6E-28
score: 99.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 46..75
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 31..83
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 8..288
NoneNo IPR availablePANTHERPTHR31375:SF142POLYGALACTURONASE PLANT-LIKE PROTEINcoord: 8..288
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 289..428
NoneNo IPR availablePANTHERPTHR31375:SF142POLYGALACTURONASE PLANT-LIKE PROTEINcoord: 289..428
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 86..430

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g238380.m01Csor.00g238380.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity