Csor.00g237890 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g237890
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionPectinesterase
LocationCsor_Chr08: 7904583 .. 7906336 (-)
RNA-Seq ExpressionCsor.00g237890
SyntenyCsor.00g237890
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintronterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTTAGTAGGCGTCTCTTCTCTGTTCCTCGTGGCCATGGTCGTTGCCGTTACCGTTGGCGTTAACAACTCCTCTCACAAAGGTGAAGGTCTCGGCGACGGTCATGGTGACGGAGCCGGAGAAATTTCTTCATCGACAAAAGCAATTCAAACTATTTGTCGGCCAACCGATTACAAAGAAACATGCGAGGAGAGTTTAATGTCTGAAGGCAGTAACACAACTGACCCTAGAGAGCTTGTAAAAATCGGCTTCAAAGTTTGCATGGACCAACTTAACTCGGCGATTAGAAATTCCACCACGCTTCAAGCATTAGCCAAAGAACCAAGAACCAACCAAGCCCTTCAGAATTGTCGCGAGCTTCTAGAATATGCTATCGACGATATAACCGATTCATTCAACCGAATCGGCACCTTTCAAATGGCCAAACTCGACAAATTCGTCGAGGATTTGTCTGTCTGGTTAAGTGGGGCCCTTACTTATGAACAAACATGTTTGGATGGTTTTGAAAACACCACCGGTGATGCCGGTGAACAAATGAAGAAGTTTTTAAAGTCAGCTCAAGAAATGACAAGTAACGGTCTTGCAATTGTAACTGAATTGAAGAACACGTTGGGATCGTTGCAAGGATCCAAGGCCGGGCGTCGGTTGTTGGCGGGCGATGAAGTGCCGTCGTGGGTTAGCGACGAAAAGAGGCGGCTGATGCAGGTGCCTACGGCGGCGATCACACCGAATGTAGTGGTGGCGCAGGACGGAAGTGGAAAATACAAGACGATTAACGAAGCGTTGGTTGAGGTGCCGAAGAAGAGCAATATAACCTTTGTGATTTACGTTAAGGCTGGAATTTATAAGGAAACTGTTCTTATTCCTAAGGCTATGACTCATGTTACAATGTACGGTGATGGCCCGACCAAAACCGTCGTGTCTGGTAGTTTGAATTTCGTCGATGGCATTCAAACCTTCAAGACAGCAACATTCGGTAAGTCAATAAAAATCTTAATTTTGTTTATATTAAAAAATATTATCTTTTCTTTTAATGTTGTTTTTGTCGCATTTAATTAGCCGTTGTCGGAGCCAATTTCTTCTGTCGAGACATGGGGTTTGAGAACACCGCTGGCTCAGCGAAACATCAAGCCGTGGCACTTCGAGTCCAATCTGATAGATCAATTTTCTTCAACTGTCATATGGACGGCTACCAAGATACTCTCTACGCCCAAACTCACCGTCAGTTCTACCGTGACTGCAAAGTCAGCGGCACCATTGACTTTGTTTTTGGGAACTCAGCCGCCGTCTTCCAAAACTGCAATTTGGTGGTGCGTAAGCCATTGGCTAACCAACAATGCATTGTCACGGCTCAAGGCCGGCTCGAGAACCGCGAACCGACAGGTCTTGTTTTCCAAAACTGCCATTTCATGGGCGACCCGGCTTACTTGCCATTCAAAGCCGTGAACAAAGCCTACTTGGGCCGTCCCTGGAAGCAATTTTCACGAACCATAATTATGGGTTCTGAAATTGACGATTTAATTCAGCCTGAGGGATGGCTGCCGTGGATGGGCGATTTTGGGCTGAACACATTGTTCTACGCCGAGTTTCAGAACAAAGGACCGGGAGCTGACCTGACCAATAGAGTGAAGTGGCAAGGAATTAAAACTCTTACGCCGCAACAGGCCGCCGATTTCACTCCCAAGATTTTTATCGACGGCGATGATTGGATTTTGAACACGGGAGTTCCGTACTCTCCTGGAATGATGTGA

mRNA sequence

ATGGCCTTAGTAGGCGTCTCTTCTCTGTTCCTCGTGGCCATGGTCGTTGCCGTTACCGTTGGCGTTAACAACTCCTCTCACAAAGGTGAAGGTCTCGGCGACGGTCATGGTGACGGAGCCGGAGAAATTTCTTCATCGACAAAAGCAATTCAAACTATTTGTCGGCCAACCGATTACAAAGAAACATGCGAGGAGAGTTTAATGTCTGAAGGCAGTAACACAACTGACCCTAGAGAGCTTGTAAAAATCGGCTTCAAAGTTTGCATGGACCAACTTAACTCGGCGATTAGAAATTCCACCACGCTTCAAGCATTAGCCAAAGAACCAAGAACCAACCAAGCCCTTCAGAATTGTCGCGAGCTTCTAGAATATGCTATCGACGATATAACCGATTCATTCAACCGAATCGGCACCTTTCAAATGGCCAAACTCGACAAATTCGTCGAGGATTTGTCTGTCTGGTTAAGTGGGGCCCTTACTTATGAACAAACATGTTTGGATGGTTTTGAAAACACCACCGGTGATGCCGGTGAACAAATGAAGAAGTTTTTAAAGTCAGCTCAAGAAATGACAAGTAACGGTCTTGCAATTGTAACTGAATTGAAGAACACGTTGGGATCGTTGCAAGGATCCAAGGCCGGGCGTCGGTTGTTGGCGGGCGATGAAGTGCCGTCGTGGGTTAGCGACGAAAAGAGGCGGCTGATGCAGGTGCCTACGGCGGCGATCACACCGAATGTAGTGGTGGCGCAGGACGGAAGTGGAAAATACAAGACGATTAACGAAGCGTTGGTTGAGGTGCCGAAGAAGAGCAATATAACCTTTGTGATTTACGTTAAGGCTGGAATTTATAAGGAAACTGTTCTTATTCCTAAGGCTATGACTCATGTTACAATGTACGGTGATGGCCCGACCAAAACCGTCGTGTCTGGTAGTTTGAATTTCGTCGATGGCATTCAAACCTTCAAGACAGCAACATTCGCCGTTGTCGGAGCCAATTTCTTCTGTCGAGACATGGGGTTTGAGAACACCGCTGGCTCAGCGAAACATCAAGCCGTGGCACTTCGAGTCCAATCTGATAGATCAATTTTCTTCAACTGTCATATGGACGGCTACCAAGATACTCTCTACGCCCAAACTCACCGTCAGTTCTACCGTGACTGCAAAGTCAGCGGCACCATTGACTTTGTTTTTGGGAACTCAGCCGCCGTCTTCCAAAACTGCAATTTGGTGGTGCGTAAGCCATTGGCTAACCAACAATGCATTGTCACGGCTCAAGGCCGGCTCGAGAACCGCGAACCGACAGGTCTTGTTTTCCAAAACTGCCATTTCATGGGCGACCCGGCTTACTTGCCATTCAAAGCCGTGAACAAAGCCTACTTGGGCCGTCCCTGGAAGCAATTTTCACGAACCATAATTATGGGTTCTGAAATTGACGATTTAATTCAGCCTGAGGGATGGCTGCCGTGGATGGGCGATTTTGGGCTGAACACATTGTTCTACGCCGAGTTTCAGAACAAAGGACCGGGAGCTGACCTGACCAATAGAGTGAAGTGGCAAGGAATTAAAACTCTTACGCCGCAACAGGCCGCCGATTTCACTCCCAAGATTTTTATCGACGGCGATGATTGGATTTTGAACACGGGAGTTCCGTACTCTCCTGGAATGATGTGA

Coding sequence (CDS)

ATGGCCTTAGTAGGCGTCTCTTCTCTGTTCCTCGTGGCCATGGTCGTTGCCGTTACCGTTGGCGTTAACAACTCCTCTCACAAAGGTGAAGGTCTCGGCGACGGTCATGGTGACGGAGCCGGAGAAATTTCTTCATCGACAAAAGCAATTCAAACTATTTGTCGGCCAACCGATTACAAAGAAACATGCGAGGAGAGTTTAATGTCTGAAGGCAGTAACACAACTGACCCTAGAGAGCTTGTAAAAATCGGCTTCAAAGTTTGCATGGACCAACTTAACTCGGCGATTAGAAATTCCACCACGCTTCAAGCATTAGCCAAAGAACCAAGAACCAACCAAGCCCTTCAGAATTGTCGCGAGCTTCTAGAATATGCTATCGACGATATAACCGATTCATTCAACCGAATCGGCACCTTTCAAATGGCCAAACTCGACAAATTCGTCGAGGATTTGTCTGTCTGGTTAAGTGGGGCCCTTACTTATGAACAAACATGTTTGGATGGTTTTGAAAACACCACCGGTGATGCCGGTGAACAAATGAAGAAGTTTTTAAAGTCAGCTCAAGAAATGACAAGTAACGGTCTTGCAATTGTAACTGAATTGAAGAACACGTTGGGATCGTTGCAAGGATCCAAGGCCGGGCGTCGGTTGTTGGCGGGCGATGAAGTGCCGTCGTGGGTTAGCGACGAAAAGAGGCGGCTGATGCAGGTGCCTACGGCGGCGATCACACCGAATGTAGTGGTGGCGCAGGACGGAAGTGGAAAATACAAGACGATTAACGAAGCGTTGGTTGAGGTGCCGAAGAAGAGCAATATAACCTTTGTGATTTACGTTAAGGCTGGAATTTATAAGGAAACTGTTCTTATTCCTAAGGCTATGACTCATGTTACAATGTACGGTGATGGCCCGACCAAAACCGTCGTGTCTGGTAGTTTGAATTTCGTCGATGGCATTCAAACCTTCAAGACAGCAACATTCGCCGTTGTCGGAGCCAATTTCTTCTGTCGAGACATGGGGTTTGAGAACACCGCTGGCTCAGCGAAACATCAAGCCGTGGCACTTCGAGTCCAATCTGATAGATCAATTTTCTTCAACTGTCATATGGACGGCTACCAAGATACTCTCTACGCCCAAACTCACCGTCAGTTCTACCGTGACTGCAAAGTCAGCGGCACCATTGACTTTGTTTTTGGGAACTCAGCCGCCGTCTTCCAAAACTGCAATTTGGTGGTGCGTAAGCCATTGGCTAACCAACAATGCATTGTCACGGCTCAAGGCCGGCTCGAGAACCGCGAACCGACAGGTCTTGTTTTCCAAAACTGCCATTTCATGGGCGACCCGGCTTACTTGCCATTCAAAGCCGTGAACAAAGCCTACTTGGGCCGTCCCTGGAAGCAATTTTCACGAACCATAATTATGGGTTCTGAAATTGACGATTTAATTCAGCCTGAGGGATGGCTGCCGTGGATGGGCGATTTTGGGCTGAACACATTGTTCTACGCCGAGTTTCAGAACAAAGGACCGGGAGCTGACCTGACCAATAGAGTGAAGTGGCAAGGAATTAAAACTCTTACGCCGCAACAGGCCGCCGATTTCACTCCCAAGATTTTTATCGACGGCGATGATTGGATTTTGAACACGGGAGTTCCGTACTCTCCTGGAATGATGTGA

Protein sequence

MALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRTNQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQMKKFLKSAQEMTSNGLAIVTELKNTLGSLQGSKAGRRLLAGDEVPSWVSDEKRRLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLIPKAMTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAVALRVQSDRSIFFNCHMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIFIDGDDWILNTGVPYSPGMM
Homology
BLAST of Csor.00g237890 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 652.1 bits (1681), Expect = 5.5e-186
Identity = 304/556 (54.68%), Postives = 406/556 (73.02%), Query Frame = 0

Query: 3   LVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYKET 62
           ++ +SS+ L++MVVAVTVGV+ + H      DG   G  E+++S KA++ +C PTDY++T
Sbjct: 17  VITISSVLLISMVVAVTVGVSLNKH------DGDSKGKAEVNASVKAVKDVCAPTDYRKT 76

Query: 63  CEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRTNQALQNCRELL 122
           CE++L+  G NTTDP ELVK  F V M Q+  A + S T+  L K+ RT  AL  C+EL+
Sbjct: 77  CEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTRMALDQCKELM 136

Query: 123 EYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQMKK 182
           +YA+D++++SF  +G F+   LD+ + +L +WLS A+++E+TCL+GF+ T G+AGE MKK
Sbjct: 137 DYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKK 196

Query: 183 FLKSAQEMTSNGLAIVTELKNTLGSLQGSKAGRRLLAGDEVPSWVSDEKRRLMQVPTA-- 242
            LK+A E+T NGLAI++E+ N +G +Q      R L  +  PSWV    R+L+Q   A  
Sbjct: 197 ALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLAEGFPSWVDQRGRKLLQAAAAYS 256

Query: 243 AITPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLIPKAMTHVTMYG 302
            + P++VVAQDGSG+YKTINEAL  VPKK N TFV+++KAG+YKE V + K M+H+   G
Sbjct: 257 DVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMSHLVFIG 316

Query: 303 DGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAVALRVQSDR 362
           DGP KT++SG+ N+ DGI T++TAT A+VG  F  +++GFENTAG+ KHQAVA+RVQSD 
Sbjct: 317 DGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAVRVQSDE 376

Query: 363 SIFFNCHMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQC 422
           SIFFNC  DGYQDTLY  +HRQF+RDC +SGTIDF+FG++AAVFQNC L+VRKPL NQ C
Sbjct: 377 SIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLPNQAC 436

Query: 423 IVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEIDDL 482
            +TA GR + RE TG VFQ C   G+P YL  K  +KAYLGRPWK++SRTIIM + I D 
Sbjct: 437 PITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMNTFIPDF 496

Query: 483 IQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIFIDG 542
           +QP+GW PW+GDFGL TLFY+E QN GPG+ L NRV W GIKTL+ +    FTP  +I G
Sbjct: 497 VQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTPAQYIQG 556

Query: 543 DDWILNTGVPYSPGMM 557
           DDWI   GVPY+ G++
Sbjct: 557 DDWIPGKGVPYTTGLL 566

BLAST of Csor.00g237890 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 1.2e-180
Identity = 299/553 (54.07%), Postives = 398/553 (71.97%), Query Frame = 0

Query: 3   LVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYKET 62
           ++ +SS+ L++MVVAVT+GV  S +K +  GD       EI++S KAI+ +C PTDYKET
Sbjct: 18  IISISSVLLISMVVAVTIGV--SVNKSDNAGD------EEITTSVKAIKDVCAPTDYKET 77

Query: 63  CEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRTNQALQNCRELL 122
           CE++L  +  +T+DP ELVK  F   M Q++   + S T+  L K+PR   AL  C+EL+
Sbjct: 78  CEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMALDQCKELM 137

Query: 123 EYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQMKK 182
           +YAI +++ SF  +G F+  K+D+ +  L +WLS  +++EQTCLDGF+ T G+AGE +KK
Sbjct: 138 DYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKK 197

Query: 183 FLKSAQEMTSNGLAIVTELKNTLGSLQGSKAGRRLLAGDEVPSWVSDEKRRLMQVPTAAI 242
            LK+A ++T NGLA+VTE+ N LG +Q  +   R L   E PSW+    RRL+  P + +
Sbjct: 198 ALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLSQEFPSWMDARARRLLNAPMSEV 257

Query: 243 TPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLIPKAMTHVTMYGDG 302
            P++VVAQDGSG+YKTINEAL  VPKK N TFV+++K GIYKE V + ++MTH+   GDG
Sbjct: 258 KPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSMTHLVFIGDG 317

Query: 303 PTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAVALRVQSDRSI 362
           P KTV+SGS ++ DGI T+KTAT A+VG +F  +++ FENTAG+ KHQAVA+RV +D SI
Sbjct: 318 PDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVAIRVLADESI 377

Query: 363 FFNCHMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQCIV 422
           F+NC  DGYQDTLYA +HRQFYRDC +SGTIDF+FG++AAVFQNC L+VRKPL NQ C +
Sbjct: 378 FYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLLNQACPI 437

Query: 423 TAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEIDDLIQ 482
           TA GR + RE TG V Q C  +G+P YL  K  +K YLGRPWK++SRTIIM + I D + 
Sbjct: 438 TAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIMNTFIPDFVP 497

Query: 483 PEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIFIDGDD 542
           PEGW PW+G+FGLNTLFY+E QN GPGA +T RV W GIK L+ ++   FTP  +I GD 
Sbjct: 498 PEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFTPAQYIQGDA 557

Query: 543 WILNTGVPYSPGM 556
           WI   GVPY  G+
Sbjct: 558 WIPGKGVPYILGL 562

BLAST of Csor.00g237890 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 3.5e-164
Identity = 281/560 (50.18%), Postives = 378/560 (67.50%), Query Frame = 0

Query: 3   LVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYKET 62
           + GV +  LV MVVAV +  + ++   E +         +I ++T A++ +C PTDYKET
Sbjct: 14  IAGVITALLVLMVVAVGITTSRNTSHSEKI------VPVQIKTATTAVEAVCAPTDYKET 73

Query: 63  CEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTT--LQALAKEPRTNQALQNCRE 122
           C  SLM    ++T P +L+K+GF V +  +  +I+ ++       A +  T  AL+ C +
Sbjct: 74  CVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALELCEK 133

Query: 123 LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQM 182
           L+  A DD+    +    F + +++ FVEDL VWLSG++ Y+QTC+D FE T     + M
Sbjct: 134 LMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLSQDM 193

Query: 183 KKFLKSAQEMTSNGLAIVTELKNTLGSLQ--------GSKAGRRLLAGDEVPSWVSDEKR 242
           +K  K+++E+TSNGLA++T + N LG           G  A + L A D +PSWV    R
Sbjct: 194 QKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAEDGIPSWVGPNTR 253

Query: 243 RLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLIPKA 302
           RLM      +  NVVVA DGSG+YKTINEAL  VPK +   FVIY+K G+Y E V + K 
Sbjct: 254 RLM-ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEKVDVTKK 313

Query: 303 MTHVTMYGDGPTKTVVSGSLNFVDG-IQTFKTATFAVVGANFFCRDMGFENTAGSAKHQA 362
           MTHVT  GDGPTKT ++GSLN+  G ++T+ TAT A+ G NF  +++GFENTAG   HQA
Sbjct: 314 MTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAGPEGHQA 373

Query: 363 VALRVQSDRSIFFNCHMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVV 422
           VALRV +D ++F+NC +DGYQDTLY  +HRQF+RDC VSGT+DF+FG+   V QNCN+VV
Sbjct: 374 VALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQNCNIVV 433

Query: 423 RKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTI 482
           RKP+ +Q C++TAQGR + RE TGLV QNCH  G+PAY+P K++NKAYLGRPWK+FSRTI
Sbjct: 434 RKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWKEFSRTI 493

Query: 483 IMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAAD 542
           IMG+ IDD+I P GWLPW GDF LNTL+YAE++N GPG++   RVKW GIK L+P+QA  
Sbjct: 494 IMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQALR 553

Query: 543 FTPKIFIDGDDWILNTGVPY 552
           FTP  F+ G+ WI    VPY
Sbjct: 554 FTPARFLRGNLWIPPNRVPY 566

BLAST of Csor.00g237890 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 531.9 bits (1369), Expect = 8.4e-150
Identity = 273/564 (48.40%), Postives = 368/564 (65.25%), Query Frame = 0

Query: 3   LVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYKET 62
           + G  S FLV MVV+V V  +  S K         D    I  +TKA+Q +C PTD+K+T
Sbjct: 13  VAGSVSGFLVIMVVSVAVVTSKHSPK---------DDENHIRKTTKAVQAVCAPTDFKDT 72

Query: 63  CEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAI-RNSTTLQALA-KEPRTNQALQNCRE 122
           C  SLM    ++ DP +L+K+GFKV +  +N ++ + S  ++A A K P    A + C +
Sbjct: 73  CVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKGAFELCEK 132

Query: 123 LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQM 182
           L+  AIDD+    +    F + +++ FVEDL VWLSG++ ++QTC+D F     +  + M
Sbjct: 133 LMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIKSNLMQDM 192

Query: 183 KKFLKSAQEMTSNGLAIVTEL-----KNTLGSLQG--SKAGRRLLA-GDEVPSWVSDEKR 242
            K  K+++E++SN LA+VT +      + L  L G  +K  R+LL+  D +P+WV  E R
Sbjct: 193 LKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTEDSIPTWVGPEAR 252

Query: 243 RLMQVPTAAITP---NVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLI 302
           RLM        P   N VVAQDG+G++KTI +AL  VPK + + F+I++K GIYKE V +
Sbjct: 253 RLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFIIHIKEGIYKEKVTV 312

Query: 303 PKAMTHVTMYGDGPTKTVVSGSLNF-VDGIQTFKTATFAVVGANFFCRDMGFENTAGSAK 362
            K M HVT  GDGP KT+++GSLNF +  ++TF TAT  + G +F  +++G ENTAG   
Sbjct: 313 TKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNIGIENTAGPEG 372

Query: 363 HQAVALRVQSDRSIFFNCHMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCN 422
            QAVALRV +D ++F +C +DG+QDTLY  +HRQFYRDC VSGT+DF+FG++  + QNC 
Sbjct: 373 GQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFGDAKCILQNCK 432

Query: 423 LVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFS 482
           +VVRKP   Q C+VTAQGR   RE TGLV   CH  GDPAY+P K+VNKAYLGRPWK+FS
Sbjct: 433 IVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKAYLGRPWKEFS 492

Query: 483 RTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQ 542
           RTIIM + IDD+I P GWLPW GDF L TL+YAE  N GPG++   RVKW GIK LTPQ 
Sbjct: 493 RTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKWPGIKKLTPQD 552

Query: 543 AADFTPKIFIDGDDWILNTGVPYS 553
           A  +T   F+ GD WI  T VPY+
Sbjct: 553 ALLYTGDRFLRGDTWIPQTQVPYT 565

BLAST of Csor.00g237890 vs. ExPASy Swiss-Prot
Match: Q42920 (Pectinesterase/pectinesterase inhibitor OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 9.0e-136
Identity = 237/450 (52.67%), Postives = 306/450 (68.00%), Query Frame = 0

Query: 115 LQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTG 174
           ++ C E+L+YA+D I  S   +  F   KL ++  DL VWL+G L+++QTCLDGF NTT 
Sbjct: 1   MEICNEVLDYAVDGIHKSVGTLDQFDFHKLSEYAFDLKVWLTGTLSHQQTCLDGFANTTT 60

Query: 175 DAGEQMKKFLKSAQEMTSNGLAIVTELKNTLGSLQGSK--AGRRLLAGDEVPSWVSDEKR 234
            AGE M K LK++ E++SN + ++  +   L     S+    RRLL+ D +PSWV+D  R
Sbjct: 61  KAGETMTKVLKTSMELSSNAIDMMDAVSRILKGFDTSQYSVSRRLLSDDGIPSWVNDGHR 120

Query: 235 RLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLIPKA 294
           RL+      + PN VVAQDGSG++KT+ +AL  VP K+ + FVI+VKAG+YKETV + K 
Sbjct: 121 RLL--AGGNVQPNAVVAQDGSGQFKTLTDALKTVPPKNAVPFVIHVKAGVYKETVNVAKE 180

Query: 295 MTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAV 354
           M +VT+ GDGPTKT  +GSLN+ DGI T+ TATF V GANF  +D+GFENTAG+ KHQAV
Sbjct: 181 MNYVTVIGDGPTKTKFTGSLNYADGINTYNTATFGVNGANFMAKDIGFENTAGTGKHQAV 240

Query: 355 ALRVQSDRSIFFNCHMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVR 414
           ALRV +D++IF+NC MDG+QDTLY Q+ RQFYRDC +SGTIDFVFG    VFQNC LV R
Sbjct: 241 ALRVTADQAIFYNCQMDGFQDTLYVQSQRQFYRDCSISGTIDFVFGERFGVFQNCKLVCR 300

Query: 415 KPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTII 474
            P   QQC+VTA GR +    + LVFQ+ HF G+PA L       +YLGRPWKQ+S+ +I
Sbjct: 301 LPAKGQQCLVTAGGREKQNSASALVFQSSHFTGEPA-LTSVTPKVSYLGRPWKQYSKVVI 360

Query: 475 MGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADF 534
           M S ID +  PEG++PWMG     T  Y E+ NKGPGAD   RVKW G+K LT   AA++
Sbjct: 361 MDSTIDAIFVPEGYMPWMGSAFKETCTYYEYNNKGPGADTNLRVKWHGVKVLTSNVAAEY 420

Query: 535 TPKIFID------GDDWILNTGVPYSPGMM 557
            P  F +       D WI+ +GVPYS G M
Sbjct: 421 YPGKFFEIVNATARDTWIVKSGVPYSLGPM 447

BLAST of Csor.00g237890 vs. NCBI nr
Match: KAG6593983.1 (putative pectinesterase/pectinesterase inhibitor 21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1118 bits (2891), Expect = 0.0
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 0

Query: 1   MALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYK 60
           MALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYK
Sbjct: 1   MALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYK 60

Query: 61  ETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRTNQALQNCRE 120
           ETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRTNQALQNCRE
Sbjct: 61  ETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRTNQALQNCRE 120

Query: 121 LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQM 180
           LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQM
Sbjct: 121 LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQM 180

Query: 181 KKFLKSAQEMTSNGLAIVTELKNTLGSLQGSKAGRRLLAGDEVPSWVSDEKRRLMQVPTA 240
           KKFLKSAQEMTSNGLAIVTELKNTLGSLQGSKAGRRLLAGDEVPSWVSDEKRRLMQVPTA
Sbjct: 181 KKFLKSAQEMTSNGLAIVTELKNTLGSLQGSKAGRRLLAGDEVPSWVSDEKRRLMQVPTA 240

Query: 241 AITPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLIPKAMTHVTMYG 300
           AITPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLIPKAMTHVTMYG
Sbjct: 241 AITPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLIPKAMTHVTMYG 300

Query: 301 DGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAVALRVQSDR 360
           DGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAVALRVQSDR
Sbjct: 301 DGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAVALRVQSDR 360

Query: 361 SIFFNCHMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQC 420
           SIFFNCHMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQC
Sbjct: 361 SIFFNCHMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQC 420

Query: 421 IVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEIDDL 480
           IVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEIDDL
Sbjct: 421 IVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEIDDL 480

Query: 481 IQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIFIDG 540
           IQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIFIDG
Sbjct: 481 IQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIFIDG 540

Query: 541 DDWILNTGVPYSPGMM 556
           DDWILNTGVPYSPGMM
Sbjct: 541 DDWILNTGVPYSPGMM 556

BLAST of Csor.00g237890 vs. NCBI nr
Match: XP_022930359.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita moschata])

HSP 1 Score: 1103 bits (2853), Expect = 0.0
Identity = 549/556 (98.74%), Postives = 552/556 (99.28%), Query Frame = 0

Query: 1   MALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYK 60
           MALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLG+GHGDGAGEISSSTKAIQTICRPTDYK
Sbjct: 16  MALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEISSSTKAIQTICRPTDYK 75

Query: 61  ETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRTNQALQNCRE 120
           ETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPR NQALQNCRE
Sbjct: 76  ETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRANQALQNCRE 135

Query: 121 LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQM 180
           LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQM
Sbjct: 136 LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQM 195

Query: 181 KKFLKSAQEMTSNGLAIVTELKNTLGSLQGSKAGRRLLAGDEVPSWVSDEKRRLMQVPTA 240
           KKFLKSAQEMTSNGLAIVTELKN LGSLQGSKAGRRLLAGDEVPSWVSDEKRRLMQVPTA
Sbjct: 196 KKFLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRRLLAGDEVPSWVSDEKRRLMQVPTA 255

Query: 241 AITPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLIPKAMTHVTMYG 300
           AITPNVVVAQDGSGKYKTINEALVEV KKSNITFVIYVKAGIYKETVLIPKAMTHVTMYG
Sbjct: 256 AITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKAGIYKETVLIPKAMTHVTMYG 315

Query: 301 DGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAVALRVQSDR 360
           DGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAG+ KHQAVALRVQSDR
Sbjct: 316 DGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGATKHQAVALRVQSDR 375

Query: 361 SIFFNCHMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQC 420
           SIFFNCHMDG+QDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQC
Sbjct: 376 SIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQC 435

Query: 421 IVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEIDDL 480
           IVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEIDDL
Sbjct: 436 IVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEIDDL 495

Query: 481 IQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIFIDG 540
           IQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIFIDG
Sbjct: 496 IQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIFIDG 555

Query: 541 DDWILNTGVPYSPGMM 556
           DDWILNTGVPYSPGMM
Sbjct: 556 DDWILNTGVPYSPGMM 571

BLAST of Csor.00g237890 vs. NCBI nr
Match: XP_023515122.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1102 bits (2850), Expect = 0.0
Identity = 547/556 (98.38%), Postives = 552/556 (99.28%), Query Frame = 0

Query: 1   MALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYK 60
           MALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYK
Sbjct: 16  MALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYK 75

Query: 61  ETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRTNQALQNCRE 120
           ETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRTNQALQNCRE
Sbjct: 76  ETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRTNQALQNCRE 135

Query: 121 LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQM 180
           LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQM
Sbjct: 136 LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQM 195

Query: 181 KKFLKSAQEMTSNGLAIVTELKNTLGSLQGSKAGRRLLAGDEVPSWVSDEKRRLMQVPTA 240
           KKFLKSAQEMTSNGLAIVTELKN LGSLQGSK GRRLLAGDEVPSWV+DEKRRLMQ+PTA
Sbjct: 196 KKFLKSAQEMTSNGLAIVTELKNMLGSLQGSKKGRRLLAGDEVPSWVTDEKRRLMQLPTA 255

Query: 241 AITPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLIPKAMTHVTMYG 300
           AITPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLIPKA+THVTMYG
Sbjct: 256 AITPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLIPKALTHVTMYG 315

Query: 301 DGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAVALRVQSDR 360
           DGPTKTVVSG LNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAVALRVQSDR
Sbjct: 316 DGPTKTVVSGGLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAVALRVQSDR 375

Query: 361 SIFFNCHMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQC 420
           SIFFNC MDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNC+LVVRKPLANQQC
Sbjct: 376 SIFFNCQMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCSLVVRKPLANQQC 435

Query: 421 IVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEIDDL 480
           IVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEIDDL
Sbjct: 436 IVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEIDDL 495

Query: 481 IQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIFIDG 540
           IQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKW+GIKTLTPQQAADFTPKIFIDG
Sbjct: 496 IQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWKGIKTLTPQQAADFTPKIFIDG 555

Query: 541 DDWILNTGVPYSPGMM 556
           DDWILNTGVPYSPGMM
Sbjct: 556 DDWILNTGVPYSPGMM 571

BLAST of Csor.00g237890 vs. NCBI nr
Match: XP_023000149.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita maxima])

HSP 1 Score: 1076 bits (2782), Expect = 0.0
Identity = 532/556 (95.68%), Postives = 544/556 (97.84%), Query Frame = 0

Query: 1   MALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYK 60
           MA VGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYK
Sbjct: 16  MAFVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYK 75

Query: 61  ETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRTNQALQNCRE 120
           ETCE+SLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRTNQALQNCRE
Sbjct: 76  ETCEKSLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRTNQALQNCRE 135

Query: 121 LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQM 180
           LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLS+WLSGALTYEQTCLDGFENTTGDAGE+M
Sbjct: 136 LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSIWLSGALTYEQTCLDGFENTTGDAGERM 195

Query: 181 KKFLKSAQEMTSNGLAIVTELKNTLGSLQGSKAGRRLLAGDEVPSWVSDEKRRLMQVPTA 240
           K FLKSAQEMTSNGLAIVTELKN LGSLQGSKAGRRLLAGDEVPSWVS EKRRLMQ+P +
Sbjct: 196 KMFLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRRLLAGDEVPSWVSSEKRRLMQLPRS 255

Query: 241 AITPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLIPKAMTHVTMYG 300
           AITPNVVVAQDGSGKYKTINEALV+VPKKSNITFVIYVKAGIYKETVLIPKA+THVTMYG
Sbjct: 256 AITPNVVVAQDGSGKYKTINEALVDVPKKSNITFVIYVKAGIYKETVLIPKALTHVTMYG 315

Query: 301 DGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAVALRVQSDR 360
           DGPTKT+VSG+LNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAVALRVQSDR
Sbjct: 316 DGPTKTIVSGALNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAVALRVQSDR 375

Query: 361 SIFFNCHMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQC 420
           SIFFNC MDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQC
Sbjct: 376 SIFFNCQMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQC 435

Query: 421 IVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEIDDL 480
           IVTAQGRLE REPTG VFQ CH MGDPAYLPFKAVNKAYLGRPWKQ+SRTIIMGSEIDDL
Sbjct: 436 IVTAQGRLEAREPTGFVFQKCHLMGDPAYLPFKAVNKAYLGRPWKQYSRTIIMGSEIDDL 495

Query: 481 IQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIFIDG 540
           IQPEGWLPWMGDFGLN+LFYAEFQNKGPGADLTNRVKWQGIKTLT QQAADFTPK+FIDG
Sbjct: 496 IQPEGWLPWMGDFGLNSLFYAEFQNKGPGADLTNRVKWQGIKTLTAQQAADFTPKVFIDG 555

Query: 541 DDWILNTGVPYSPGMM 556
           D WILNTGVPYSPGMM
Sbjct: 556 DAWILNTGVPYSPGMM 571

BLAST of Csor.00g237890 vs. NCBI nr
Match: XP_008458409.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo])

HSP 1 Score: 868 bits (2242), Expect = 2.26e-313
Identity = 423/558 (75.81%), Postives = 478/558 (85.66%), Query Frame = 0

Query: 2   ALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYKE 61
           A++ VSSL LVAMVVAVTVG+NN+ ++  G GD       EISSSTKAIQ IC+PTDYKE
Sbjct: 18  AIISVSSLILVAMVVAVTVGINNAPYEEHGEGDQGSKTTPEISSSTKAIQAICQPTDYKE 77

Query: 62  TCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRTNQALQNCREL 121
           TCE+SLM+E  NTTDP+ELVK+GFK+    LN AIRNSTTLQ L+K+PRTNQALQNCREL
Sbjct: 78  TCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQELSKDPRTNQALQNCREL 137

Query: 122 LEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQMK 181
           LEYAIDD+ +SF RIGTFQM+KLD+FV DL +WLSGALTYEQTCLDGFENTTGDAG +M+
Sbjct: 138 LEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQTCLDGFENTTGDAGTRMQ 197

Query: 182 KFLKSAQEMTSNGLAIVTELKNTLGSLQ-GSKAGRRLLAGD--EVPSWVSDEKRRLMQVP 241
           +FLKSAQEMT+NGL IV EL   LGSLQ     GRRLL  D  E+PSW+SD KRRLMQ  
Sbjct: 198 EFLKSAQEMTTNGLGIVNELSTILGSLQIPGMTGRRLLEDDDHEMPSWISDGKRRLMQAG 257

Query: 242 TAAITPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLIPKAMTHVTM 301
            A + P+++VAQDGSGKYKTIN AL +VP KSN TFVIYVKAGIYKE V+IPK MTH+TM
Sbjct: 258 AAGMKPDLIVAQDGSGKYKTINAALADVPLKSNKTFVIYVKAGIYKEIVVIPKHMTHLTM 317

Query: 302 YGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAVALRVQS 361
           YGDGPTKTVV+GSLNF+DGIQTFKTATF+ +GANF+ RDMGFENTAGSAKHQAVALRVQS
Sbjct: 318 YGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFENTAGSAKHQAVALRVQS 377

Query: 362 DRSIFFNCHMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQ 421
           DRSIF+NC +DGYQDTLYA  HRQFYRDC +SGTIDFVFGN+A  FQNC LVVRKP+ NQ
Sbjct: 378 DRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAATNFQNCKLVVRKPMDNQ 437

Query: 422 QCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEID 481
           QCIVTA GR+  +EPT L+FQ CHFMGDPAYLPFK++NKAYLGRPWKQ+SRTII+GS+ID
Sbjct: 438 QCIVTAHGRMNRKEPTALIFQGCHFMGDPAYLPFKSINKAYLGRPWKQYSRTIIIGSQID 497

Query: 482 DLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIFI 541
           D+IQPEGWLPWMGDFGLNTLFYAE QNKGPGA  T RVKW+GIK +TPQ AADFTP+ FI
Sbjct: 498 DIIQPEGWLPWMGDFGLNTLFYAEVQNKGPGAVETKRVKWRGIKHITPQHAADFTPRRFI 557

Query: 542 DGDDWILNTGVPYSPGMM 556
           DGD WI   G+PYS GMM
Sbjct: 558 DGDAWIPAKGIPYSSGMM 575

BLAST of Csor.00g237890 vs. ExPASy TrEMBL
Match: A0A6J1ER80 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111436828 PE=3 SV=1)

HSP 1 Score: 1103 bits (2853), Expect = 0.0
Identity = 549/556 (98.74%), Postives = 552/556 (99.28%), Query Frame = 0

Query: 1   MALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYK 60
           MALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLG+GHGDGAGEISSSTKAIQTICRPTDYK
Sbjct: 16  MALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGNGHGDGAGEISSSTKAIQTICRPTDYK 75

Query: 61  ETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRTNQALQNCRE 120
           ETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPR NQALQNCRE
Sbjct: 76  ETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRANQALQNCRE 135

Query: 121 LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQM 180
           LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQM
Sbjct: 136 LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQM 195

Query: 181 KKFLKSAQEMTSNGLAIVTELKNTLGSLQGSKAGRRLLAGDEVPSWVSDEKRRLMQVPTA 240
           KKFLKSAQEMTSNGLAIVTELKN LGSLQGSKAGRRLLAGDEVPSWVSDEKRRLMQVPTA
Sbjct: 196 KKFLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRRLLAGDEVPSWVSDEKRRLMQVPTA 255

Query: 241 AITPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLIPKAMTHVTMYG 300
           AITPNVVVAQDGSGKYKTINEALVEV KKSNITFVIYVKAGIYKETVLIPKAMTHVTMYG
Sbjct: 256 AITPNVVVAQDGSGKYKTINEALVEVLKKSNITFVIYVKAGIYKETVLIPKAMTHVTMYG 315

Query: 301 DGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAVALRVQSDR 360
           DGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAG+ KHQAVALRVQSDR
Sbjct: 316 DGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGATKHQAVALRVQSDR 375

Query: 361 SIFFNCHMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQC 420
           SIFFNCHMDG+QDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQC
Sbjct: 376 SIFFNCHMDGHQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQC 435

Query: 421 IVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEIDDL 480
           IVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEIDDL
Sbjct: 436 IVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEIDDL 495

Query: 481 IQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIFIDG 540
           IQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIFIDG
Sbjct: 496 IQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIFIDG 555

Query: 541 DDWILNTGVPYSPGMM 556
           DDWILNTGVPYSPGMM
Sbjct: 556 DDWILNTGVPYSPGMM 571

BLAST of Csor.00g237890 vs. ExPASy TrEMBL
Match: A0A6J1KJ31 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111494433 PE=3 SV=1)

HSP 1 Score: 1076 bits (2782), Expect = 0.0
Identity = 532/556 (95.68%), Postives = 544/556 (97.84%), Query Frame = 0

Query: 1   MALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYK 60
           MA VGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYK
Sbjct: 16  MAFVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYK 75

Query: 61  ETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRTNQALQNCRE 120
           ETCE+SLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRTNQALQNCRE
Sbjct: 76  ETCEKSLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRTNQALQNCRE 135

Query: 121 LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQM 180
           LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLS+WLSGALTYEQTCLDGFENTTGDAGE+M
Sbjct: 136 LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSIWLSGALTYEQTCLDGFENTTGDAGERM 195

Query: 181 KKFLKSAQEMTSNGLAIVTELKNTLGSLQGSKAGRRLLAGDEVPSWVSDEKRRLMQVPTA 240
           K FLKSAQEMTSNGLAIVTELKN LGSLQGSKAGRRLLAGDEVPSWVS EKRRLMQ+P +
Sbjct: 196 KMFLKSAQEMTSNGLAIVTELKNMLGSLQGSKAGRRLLAGDEVPSWVSSEKRRLMQLPRS 255

Query: 241 AITPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLIPKAMTHVTMYG 300
           AITPNVVVAQDGSGKYKTINEALV+VPKKSNITFVIYVKAGIYKETVLIPKA+THVTMYG
Sbjct: 256 AITPNVVVAQDGSGKYKTINEALVDVPKKSNITFVIYVKAGIYKETVLIPKALTHVTMYG 315

Query: 301 DGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAVALRVQSDR 360
           DGPTKT+VSG+LNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAVALRVQSDR
Sbjct: 316 DGPTKTIVSGALNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAVALRVQSDR 375

Query: 361 SIFFNCHMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQC 420
           SIFFNC MDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQC
Sbjct: 376 SIFFNCQMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQC 435

Query: 421 IVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEIDDL 480
           IVTAQGRLE REPTG VFQ CH MGDPAYLPFKAVNKAYLGRPWKQ+SRTIIMGSEIDDL
Sbjct: 436 IVTAQGRLEAREPTGFVFQKCHLMGDPAYLPFKAVNKAYLGRPWKQYSRTIIMGSEIDDL 495

Query: 481 IQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIFIDG 540
           IQPEGWLPWMGDFGLN+LFYAEFQNKGPGADLTNRVKWQGIKTLT QQAADFTPK+FIDG
Sbjct: 496 IQPEGWLPWMGDFGLNSLFYAEFQNKGPGADLTNRVKWQGIKTLTAQQAADFTPKVFIDG 555

Query: 541 DDWILNTGVPYSPGMM 556
           D WILNTGVPYSPGMM
Sbjct: 556 DAWILNTGVPYSPGMM 571

BLAST of Csor.00g237890 vs. ExPASy TrEMBL
Match: A0A1S3C7B6 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497833 PE=3 SV=1)

HSP 1 Score: 868 bits (2242), Expect = 1.09e-313
Identity = 423/558 (75.81%), Postives = 478/558 (85.66%), Query Frame = 0

Query: 2   ALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYKE 61
           A++ VSSL LVAMVVAVTVG+NN+ ++  G GD       EISSSTKAIQ IC+PTDYKE
Sbjct: 18  AIISVSSLILVAMVVAVTVGINNAPYEEHGEGDQGSKTTPEISSSTKAIQAICQPTDYKE 77

Query: 62  TCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRTNQALQNCREL 121
           TCE+SLM+E  NTTDP+ELVK+GFK+    LN AIRNSTTLQ L+K+PRTNQALQNCREL
Sbjct: 78  TCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQELSKDPRTNQALQNCREL 137

Query: 122 LEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQMK 181
           LEYAIDD+ +SF RIGTFQM+KLD+FV DL +WLSGALTYEQTCLDGFENTTGDAG +M+
Sbjct: 138 LEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQTCLDGFENTTGDAGTRMQ 197

Query: 182 KFLKSAQEMTSNGLAIVTELKNTLGSLQ-GSKAGRRLLAGD--EVPSWVSDEKRRLMQVP 241
           +FLKSAQEMT+NGL IV EL   LGSLQ     GRRLL  D  E+PSW+SD KRRLMQ  
Sbjct: 198 EFLKSAQEMTTNGLGIVNELSTILGSLQIPGMTGRRLLEDDDHEMPSWISDGKRRLMQAG 257

Query: 242 TAAITPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLIPKAMTHVTM 301
            A + P+++VAQDGSGKYKTIN AL +VP KSN TFVIYVKAGIYKE V+IPK MTH+TM
Sbjct: 258 AAGMKPDLIVAQDGSGKYKTINAALADVPLKSNKTFVIYVKAGIYKEIVVIPKHMTHLTM 317

Query: 302 YGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAVALRVQS 361
           YGDGPTKTVV+GSLNF+DGIQTFKTATF+ +GANF+ RDMGFENTAGSAKHQAVALRVQS
Sbjct: 318 YGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFENTAGSAKHQAVALRVQS 377

Query: 362 DRSIFFNCHMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQ 421
           DRSIF+NC +DGYQDTLYA  HRQFYRDC +SGTIDFVFGN+A  FQNC LVVRKP+ NQ
Sbjct: 378 DRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAATNFQNCKLVVRKPMDNQ 437

Query: 422 QCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEID 481
           QCIVTA GR+  +EPT L+FQ CHFMGDPAYLPFK++NKAYLGRPWKQ+SRTII+GS+ID
Sbjct: 438 QCIVTAHGRMNRKEPTALIFQGCHFMGDPAYLPFKSINKAYLGRPWKQYSRTIIIGSQID 497

Query: 482 DLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIFI 541
           D+IQPEGWLPWMGDFGLNTLFYAE QNKGPGA  T RVKW+GIK +TPQ AADFTP+ FI
Sbjct: 498 DIIQPEGWLPWMGDFGLNTLFYAEVQNKGPGAVETKRVKWRGIKHITPQHAADFTPRRFI 557

Query: 542 DGDDWILNTGVPYSPGMM 556
           DGD WI   G+PYS GMM
Sbjct: 558 DGDAWIPAKGIPYSSGMM 575

BLAST of Csor.00g237890 vs. ExPASy TrEMBL
Match: A0A0A0KD24 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G318700 PE=3 SV=1)

HSP 1 Score: 860 bits (2223), Expect = 8.83e-311
Identity = 425/559 (76.03%), Postives = 478/559 (85.51%), Query Frame = 0

Query: 2   ALVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHG-DGAGEISSSTKAIQTICRPTDYK 61
           A++ VSSL LVAMVVAVTVGVNN+ H  E   D HG +   EI+SSTKAIQ IC+PTDYK
Sbjct: 18  AIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEITSSTKAIQAICQPTDYK 77

Query: 62  ETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRTNQALQNCRE 121
           ETCE+SL +E  NTTDP+ELVK+GFK+    LN AI+NSTTL+ LAK+PRTNQALQNCRE
Sbjct: 78  ETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLKELAKDPRTNQALQNCRE 137

Query: 122 LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQM 181
           LLEYAIDD+  SF RIGTFQM+KLD FV DL +WLSGALTYEQTCLDGFENTTGDAG +M
Sbjct: 138 LLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQTCLDGFENTTGDAGVRM 197

Query: 182 KKFLKSAQEMTSNGLAIVTELKNTLGSLQ-GSKAGRRLLAGD--EVPSWVSDEKRRLMQV 241
           ++FLKSAQ+MT+NGL IV EL   LGSLQ    +GRRLL  D  E+PSWVSD KRRLMQ 
Sbjct: 198 QEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSGRRLLEDDDREMPSWVSDGKRRLMQA 257

Query: 242 PTAAITPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLIPKAMTHVT 301
             AA+ P++VVAQDGSGKYKTIN AL +VP KSN TFVI+VKAGIYKE V+IPK MTH+T
Sbjct: 258 GAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVKAGIYKEIVVIPKHMTHLT 317

Query: 302 MYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAVALRVQ 361
           MYGDGPTKTVV+GSLNF+DGIQTFKTATF+ +GANF+ RDMGFENTAG+AKHQAVALRVQ
Sbjct: 318 MYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFENTAGAAKHQAVALRVQ 377

Query: 362 SDRSIFFNCHMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLAN 421
           SDRSIFFNC +DGYQDTLYA  HRQFYRDC +SGTIDFVFGN+A  FQNC LVVRKPL N
Sbjct: 378 SDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAATNFQNCKLVVRKPLDN 437

Query: 422 QQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEI 481
           QQCIVTA GRL  +EPT L+FQ+CHFMGDPAYLPFKA+NKAYLGRPWK++SRTII+GS I
Sbjct: 438 QQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKAYLGRPWKEYSRTIIIGSTI 497

Query: 482 DDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIF 541
           DD+IQPEGWLPWMGDFGLNTLFYAE QNKG GAD + RVKW+GIK +TPQ AADFTP+ F
Sbjct: 498 DDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKWRGIKHITPQHAADFTPRRF 557

Query: 542 IDGDDWILNTGVPYSPGMM 556
           IDGD WI   G+PYS GMM
Sbjct: 558 IDGDAWIPAKGIPYSSGMM 576

BLAST of Csor.00g237890 vs. ExPASy TrEMBL
Match: A0A6J1H4H8 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460411 PE=3 SV=1)

HSP 1 Score: 852 bits (2200), Expect = 3.12e-307
Identity = 425/564 (75.35%), Postives = 481/564 (85.28%), Query Frame = 0

Query: 2   ALVGVSSLFLVAMVVAVTVGVNNSSHK------GEGLGDGH--GDGAGEISSSTKAIQTI 61
           A++ VSSL LVAMVVAVTVGVNN+  +      G G GD H  GD A +ISSSTKAI+TI
Sbjct: 17  AVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHGDDAEKISSSTKAIKTI 76

Query: 62  CRPTDYKETCEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRTNQ 121
           C+PTDYKETCEESLM E  NTTDP+ELVK GFK+ +++LN+AIRNSTTLQ LA++PR NQ
Sbjct: 77  CQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIRNSTTLQELAQDPRANQ 136

Query: 122 ALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTT 181
           ALQNCRELLEYAIDD+ DS++RIGTF ++K ++FV DL +WLS ALTYEQTCLDGFENTT
Sbjct: 137 ALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSAALTYEQTCLDGFENTT 196

Query: 182 GDAGEQMKKFLKSAQEMTSNGLAIVTELKNTLGSLQGSKA-GRRLLAGDEVPSWVSDEKR 241
           GD+GE+MK+FLKSAQEMT+NGL IV+EL   LG+L    A GRRLL  D +P WVSD KR
Sbjct: 197 GDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLDAPAATGRRLLDTD-IPLWVSDGKR 256

Query: 242 RLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLIPKA 301
           RLMQV T AI P++VVAQDGSGKYKTINEALV VP KSN TFVIYVKAGIYKE V+IPK 
Sbjct: 257 RLMQVGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKTFVIYVKAGIYKEIVVIPKH 316

Query: 302 MTHVTMYGDGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAV 361
           MTH+TMYGDGPTK+VV+G+LNFVDGIQTFKTATFA +GANFF RDMGF NTAG  KHQAV
Sbjct: 317 MTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVRDMGFVNTAGPTKHQAV 376

Query: 362 ALRVQSDRSIFFNCHMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVR 421
           ALRVQSDR+I +NC +DGYQDTLYA  +RQFYRDC +SGTIDFVFGNSAA+FQNC LVVR
Sbjct: 377 ALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFVFGNSAAIFQNCKLVVR 436

Query: 422 KPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTII 481
           KPL NQQCIVTA GRL  REPT L+FQ CHFMGDP+YLPFK VNKAYLGRPWKQ+SRTII
Sbjct: 437 KPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPFKKVNKAYLGRPWKQYSRTII 496

Query: 482 MGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADF 541
           MGS+IDDLIQPEGWLPWMGDF LNTLFYAE QN+GPGAD ++RVKW+GI+ +TPQ AA +
Sbjct: 497 MGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRVKWRGIRHITPQHAAAY 556

Query: 542 TPKIFIDGDDWILNTGVPYSPGMM 556
           T + FIDGD WI   GVPYS GMM
Sbjct: 557 TARRFIDGDAWIPVRGVPYSSGMM 579

BLAST of Csor.00g237890 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 652.1 bits (1681), Expect = 3.9e-187
Identity = 304/556 (54.68%), Postives = 406/556 (73.02%), Query Frame = 0

Query: 3   LVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYKET 62
           ++ +SS+ L++MVVAVTVGV+ + H      DG   G  E+++S KA++ +C PTDY++T
Sbjct: 17  VITISSVLLISMVVAVTVGVSLNKH------DGDSKGKAEVNASVKAVKDVCAPTDYRKT 76

Query: 63  CEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRTNQALQNCRELL 122
           CE++L+  G NTTDP ELVK  F V M Q+  A + S T+  L K+ RT  AL  C+EL+
Sbjct: 77  CEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTRMALDQCKELM 136

Query: 123 EYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQMKK 182
           +YA+D++++SF  +G F+   LD+ + +L +WLS A+++E+TCL+GF+ T G+AGE MKK
Sbjct: 137 DYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKK 196

Query: 183 FLKSAQEMTSNGLAIVTELKNTLGSLQGSKAGRRLLAGDEVPSWVSDEKRRLMQVPTA-- 242
            LK+A E+T NGLAI++E+ N +G +Q      R L  +  PSWV    R+L+Q   A  
Sbjct: 197 ALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLAEGFPSWVDQRGRKLLQAAAAYS 256

Query: 243 AITPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLIPKAMTHVTMYG 302
            + P++VVAQDGSG+YKTINEAL  VPKK N TFV+++KAG+YKE V + K M+H+   G
Sbjct: 257 DVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMSHLVFIG 316

Query: 303 DGPTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAVALRVQSDR 362
           DGP KT++SG+ N+ DGI T++TAT A+VG  F  +++GFENTAG+ KHQAVA+RVQSD 
Sbjct: 317 DGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAVRVQSDE 376

Query: 363 SIFFNCHMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQC 422
           SIFFNC  DGYQDTLY  +HRQF+RDC +SGTIDF+FG++AAVFQNC L+VRKPL NQ C
Sbjct: 377 SIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLPNQAC 436

Query: 423 IVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEIDDL 482
            +TA GR + RE TG VFQ C   G+P YL  K  +KAYLGRPWK++SRTIIM + I D 
Sbjct: 437 PITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMNTFIPDF 496

Query: 483 IQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIFIDG 542
           +QP+GW PW+GDFGL TLFY+E QN GPG+ L NRV W GIKTL+ +    FTP  +I G
Sbjct: 497 VQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTPAQYIQG 556

Query: 543 DDWILNTGVPYSPGMM 557
           DDWI   GVPY+ G++
Sbjct: 557 DDWIPGKGVPYTTGLL 566

BLAST of Csor.00g237890 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 634.4 bits (1635), Expect = 8.5e-182
Identity = 299/553 (54.07%), Postives = 398/553 (71.97%), Query Frame = 0

Query: 3   LVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYKET 62
           ++ +SS+ L++MVVAVT+GV  S +K +  GD       EI++S KAI+ +C PTDYKET
Sbjct: 18  IISISSVLLISMVVAVTIGV--SVNKSDNAGD------EEITTSVKAIKDVCAPTDYKET 77

Query: 63  CEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAKEPRTNQALQNCRELL 122
           CE++L  +  +T+DP ELVK  F   M Q++   + S T+  L K+PR   AL  C+EL+
Sbjct: 78  CEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMALDQCKELM 137

Query: 123 EYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQMKK 182
           +YAI +++ SF  +G F+  K+D+ +  L +WLS  +++EQTCLDGF+ T G+AGE +KK
Sbjct: 138 DYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKK 197

Query: 183 FLKSAQEMTSNGLAIVTELKNTLGSLQGSKAGRRLLAGDEVPSWVSDEKRRLMQVPTAAI 242
            LK+A ++T NGLA+VTE+ N LG +Q  +   R L   E PSW+    RRL+  P + +
Sbjct: 198 ALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLSQEFPSWMDARARRLLNAPMSEV 257

Query: 243 TPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLIPKAMTHVTMYGDG 302
            P++VVAQDGSG+YKTINEAL  VPKK N TFV+++K GIYKE V + ++MTH+   GDG
Sbjct: 258 KPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSMTHLVFIGDG 317

Query: 303 PTKTVVSGSLNFVDGIQTFKTATFAVVGANFFCRDMGFENTAGSAKHQAVALRVQSDRSI 362
           P KTV+SGS ++ DGI T+KTAT A+VG +F  +++ FENTAG+ KHQAVA+RV +D SI
Sbjct: 318 PDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVAIRVLADESI 377

Query: 363 FFNCHMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVVRKPLANQQCIV 422
           F+NC  DGYQDTLYA +HRQFYRDC +SGTIDF+FG++AAVFQNC L+VRKPL NQ C +
Sbjct: 378 FYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLLNQACPI 437

Query: 423 TAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTIIMGSEIDDLIQ 482
           TA GR + RE TG V Q C  +G+P YL  K  +K YLGRPWK++SRTIIM + I D + 
Sbjct: 438 TAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIMNTFIPDFVP 497

Query: 483 PEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAADFTPKIFIDGDD 542
           PEGW PW+G+FGLNTLFY+E QN GPGA +T RV W GIK L+ ++   FTP  +I GD 
Sbjct: 498 PEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFTPAQYIQGDA 557

Query: 543 WILNTGVPYSPGM 556
           WI   GVPY  G+
Sbjct: 558 WIPGKGVPYILGL 562

BLAST of Csor.00g237890 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 579.7 bits (1493), Expect = 2.5e-165
Identity = 281/560 (50.18%), Postives = 378/560 (67.50%), Query Frame = 0

Query: 3   LVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYKET 62
           + GV +  LV MVVAV +  + ++   E +         +I ++T A++ +C PTDYKET
Sbjct: 14  IAGVITALLVLMVVAVGITTSRNTSHSEKI------VPVQIKTATTAVEAVCAPTDYKET 73

Query: 63  CEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTT--LQALAKEPRTNQALQNCRE 122
           C  SLM    ++T P +L+K+GF V +  +  +I+ ++       A +  T  AL+ C +
Sbjct: 74  CVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALELCEK 133

Query: 123 LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQM 182
           L+  A DD+    +    F + +++ FVEDL VWLSG++ Y+QTC+D FE T     + M
Sbjct: 134 LMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLSQDM 193

Query: 183 KKFLKSAQEMTSNGLAIVTELKNTLGSLQ--------GSKAGRRLLAGDEVPSWVSDEKR 242
           +K  K+++E+TSNGLA++T + N LG           G  A + L A D +PSWV    R
Sbjct: 194 QKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAEDGIPSWVGPNTR 253

Query: 243 RLMQVPTAAITPNVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLIPKA 302
           RLM      +  NVVVA DGSG+YKTINEAL  VPK +   FVIY+K G+Y E V + K 
Sbjct: 254 RLM-ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEKVDVTKK 313

Query: 303 MTHVTMYGDGPTKTVVSGSLNFVDG-IQTFKTATFAVVGANFFCRDMGFENTAGSAKHQA 362
           MTHVT  GDGPTKT ++GSLN+  G ++T+ TAT A+ G NF  +++GFENTAG   HQA
Sbjct: 314 MTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAGPEGHQA 373

Query: 363 VALRVQSDRSIFFNCHMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCNLVV 422
           VALRV +D ++F+NC +DGYQDTLY  +HRQF+RDC VSGT+DF+FG+   V QNCN+VV
Sbjct: 374 VALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQNCNIVV 433

Query: 423 RKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFSRTI 482
           RKP+ +Q C++TAQGR + RE TGLV QNCH  G+PAY+P K++NKAYLGRPWK+FSRTI
Sbjct: 434 RKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWKEFSRTI 493

Query: 483 IMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQAAD 542
           IMG+ IDD+I P GWLPW GDF LNTL+YAE++N GPG++   RVKW GIK L+P+QA  
Sbjct: 494 IMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQALR 553

Query: 543 FTPKIFIDGDDWILNTGVPY 552
           FTP  F+ G+ WI    VPY
Sbjct: 554 FTPARFLRGNLWIPPNRVPY 566

BLAST of Csor.00g237890 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 531.9 bits (1369), Expect = 5.9e-151
Identity = 273/564 (48.40%), Postives = 368/564 (65.25%), Query Frame = 0

Query: 3   LVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYKET 62
           + G  S FLV MVV+V V  +  S K         D    I  +TKA+Q +C PTD+K+T
Sbjct: 13  VAGSVSGFLVIMVVSVAVVTSKHSPK---------DDENHIRKTTKAVQAVCAPTDFKDT 72

Query: 63  CEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAI-RNSTTLQALA-KEPRTNQALQNCRE 122
           C  SLM    ++ DP +L+K+GFKV +  +N ++ + S  ++A A K P    A + C +
Sbjct: 73  CVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKGAFELCEK 132

Query: 123 LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQM 182
           L+  AIDD+    +    F + +++ FVEDL VWLSG++ ++QTC+D F     +  + M
Sbjct: 133 LMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIKSNLMQDM 192

Query: 183 KKFLKSAQEMTSNGLAIVTEL-----KNTLGSLQG--SKAGRRLLA-GDEVPSWVSDEKR 242
            K  K+++E++SN LA+VT +      + L  L G  +K  R+LL+  D +P+WV  E R
Sbjct: 193 LKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTEDSIPTWVGPEAR 252

Query: 243 RLMQVPTAAITP---NVVVAQDGSGKYKTINEALVEVPKKSNITFVIYVKAGIYKETVLI 302
           RLM        P   N VVAQDG+G++KTI +AL  VPK + + F+I++K GIYKE V +
Sbjct: 253 RLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFIIHIKEGIYKEKVTV 312

Query: 303 PKAMTHVTMYGDGPTKTVVSGSLNF-VDGIQTFKTATFAVVGANFFCRDMGFENTAGSAK 362
            K M HVT  GDGP KT+++GSLNF +  ++TF TAT  + G +F  +++G ENTAG   
Sbjct: 313 TKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNIGIENTAGPEG 372

Query: 363 HQAVALRVQSDRSIFFNCHMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSAAVFQNCN 422
            QAVALRV +D ++F +C +DG+QDTLY  +HRQFYRDC VSGT+DF+FG++  + QNC 
Sbjct: 373 GQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFGDAKCILQNCK 432

Query: 423 LVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLPFKAVNKAYLGRPWKQFS 482
           +VVRKP   Q C+VTAQGR   RE TGLV   CH  GDPAY+P K+VNKAYLGRPWK+FS
Sbjct: 433 IVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKAYLGRPWKEFS 492

Query: 483 RTIIMGSEIDDLIQPEGWLPWMGDFGLNTLFYAEFQNKGPGADLTNRVKWQGIKTLTPQQ 542
           RTIIM + IDD+I P GWLPW GDF L TL+YAE  N GPG++   RVKW GIK LTPQ 
Sbjct: 493 RTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKWPGIKKLTPQD 552

Query: 543 AADFTPKIFIDGDDWILNTGVPYS 553
           A  +T   F+ GD WI  T VPY+
Sbjct: 553 ALLYTGDRFLRGDTWIPQTQVPYT 565

BLAST of Csor.00g237890 vs. TAIR 10
Match: AT4G15980.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 469.9 bits (1208), Expect = 2.8e-132
Identity = 275/701 (39.23%), Postives = 372/701 (53.07%), Query Frame = 0

Query: 3   LVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHGDGAGEISSSTKAIQTICRPTDYKET 62
           L+GV++L ++AMV+ V     NS  +   + + H +    I+ +T +I  IC  TDYK+ 
Sbjct: 6   LLGVTAL-IMAMVICVEANDGNSPSR--KMEEVHRESKLMITKTTVSI--ICASTDYKQD 65

Query: 63  CEESLMSEGSNTTDPRELVKIGFKVCMDQLNSAI-RNSTTLQALA-KEPRTNQALQNCRE 122
           C  SL +  S   DPR L++  F + +  + S I R    L++ A  +  T +AL  CRE
Sbjct: 66  CTTSLATVRS--PDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTCRE 125

Query: 123 LLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSVWLSGALTYEQTCLDGFENTTGDAGEQM 182
           L++ AIDD+  + ++   F   +L  FVEDL VWLSG++TY+QTC+DGFE    +A   M
Sbjct: 126 LMDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAVMM 185

Query: 183 KKFLKSAQEMTSNGLAIVTELKNTL------------------------------GSLQG 242
           ++ ++  Q +TSNGLAI   L   L                              GS Q 
Sbjct: 186 ERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSSQD 245

Query: 243 SKAG-------------------------------------------------------- 302
           S                                                           
Sbjct: 246 SPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKSAF 305

Query: 303 ------------------------------------------------------------ 362
                                                                       
Sbjct: 306 SGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSLKD 365

Query: 363 RRLLAGDEVPSWVSDEKRRLM--QVPTAAITPNVVVAQDGSGKYKTINEALVEVPKKSNI 422
           R L    E P WV+   RRL+  +     I  NVVVA+DGSGK KTI +AL  VP K+  
Sbjct: 366 RHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVPMKNTK 425

Query: 423 TFVIYVKAGIYKETVLIPKAMTHVTMYGDGPTKTVVSGSLNFV-DGIQTFKTATFAVVGA 482
            FVI++K G+YKE V + K M HV   GDGPTKTV++G + F+ D + T++TA+ AV G 
Sbjct: 426 KFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAVNGD 485

Query: 483 NFFCRDMGFENTAGSAKHQAVALRVQSDRSIFFNCHMDGYQDTLYAQTHRQFYRDCKVSG 542
            F  +D+GFENTAG+A+HQAVALRV +D ++FFNCHM+GYQDTLY  THRQFYR+C+VSG
Sbjct: 486 YFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCRVSG 545

Query: 543 TIDFVFGNSAAVFQNCNLVVRKPLANQQCIVTAQGRLENREPTGLVFQNCHFMGDPAYLP 553
           TIDFVFG++ AVFQNC  V+R+P+ +QQCIVTAQGR + RE TG+V  N    GD +YLP
Sbjct: 546 TIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDASYLP 605

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GX865.5e-18654.68Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3... [more]
Q3E8Z81.2e-18054.07Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3... [more]
Q9FJ213.5e-16450.18Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3... [more]
Q8GXA18.4e-15048.40Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3... [more]
Q429209.0e-13652.67Pectinesterase/pectinesterase inhibitor OS=Medicago sativa OX=3879 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6593983.10.0100.00putative pectinesterase/pectinesterase inhibitor 21, partial [Cucurbita argyrosp... [more]
XP_022930359.10.098.74probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita moschata][more]
XP_023515122.10.098.38probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita pepo subsp. pepo][more]
XP_023000149.10.095.68probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita maxima][more]
XP_008458409.12.26e-31375.81PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1ER800.098.74Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111436828 PE=3 SV=1[more]
A0A6J1KJ310.095.68Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111494433 PE=3 SV=1[more]
A0A1S3C7B61.09e-31375.81Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497833 PE=3 SV=1[more]
A0A0A0KD248.83e-31176.03Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G318700 PE=3 SV=1[more]
A0A6J1H4H83.12e-30775.35Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460411 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G05610.13.9e-18754.68Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G27870.18.5e-18254.07Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G49180.12.5e-16550.18Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G06830.15.9e-15148.40Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G15980.12.8e-13239.23Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 45..197
e-value: 1.5E-38
score: 144.1
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 46..201
e-value: 3.6E-25
score: 86.3
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 49..197
e-value: 1.2E-28
score: 100.4
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 44..203
e-value: 3.0E-36
score: 126.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 48..201
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 239..555
e-value: 9.5E-117
score: 391.9
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 245..541
e-value: 2.1E-140
score: 466.6
NoneNo IPR availablePANTHERPTHR31707:SF337PECTINESTERASEcoord: 4..555
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 4..555
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 52..201
e-value: 5.89219E-47
score: 159.526
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 389..398
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 270..289
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 240..554

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g237890.m01Csor.00g237890.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity