Csor.00g235450 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g235450
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1
Location	Csor_Chr08: 6453541 .. 6455914 (-)
RNA-Seq Expression	Csor.00g235450
Synteny	Csor.00g235450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAATAAAACACAGAAAACCTCAAAATTTGCCCCTTCTTCCCGTCACAAAACAGAGAAGGATGATGAACCAACCTGGGAAATGGGTGGCGCTGGTGGCGGCCATATGGATTCAGGCATTTACAGGCACGAATTTTGATTTCCCCTCTTATTCTTCCAATCTGAAGGCGGCTTTGGGGATGTCGCAGGTAGAGCTTAACTATCTGGCGGTGGCTTCTGACGTCGGTAAGGCCTTTGGGTGGTGCTCTGGCATCGCTCTCTTGTATTTTCCTTTGTGGGTGGTGATGCTCATGGCTGCTTCCATGGGGTTTCTTGGCTATGGCCTTCAATGGCTTCTCCTTCAAGGGATTGTATCTCTGCCTTACTCCATGGTTGGTTTCCCTTCTCATCTCTTCTCTATTGTTTACACCAAATCCACGTTGACCTGAATTGGATACATTTCCTGACAAATTGGGGAAAAGGGTTTTCTTAACTTATTAGCAGCATGAATAGATCCACACAAATATTCATAAATTGTCACATATTTCATACCCTTTTCATCGAAACTATCAAGAACGAAGATAGGTTTGAGGTTTGGGGCAGGCAAACAAATTGGAATGATTACTCTCATTTTCCTGCAGGTATATCTTCTCTGCTTGTTGGCTGGATGTAGTATTTGCTGGTTTAATACAATCTGCTATGTCTCCTGCATCCAAAAGTTTCCAACCAATCGCGCGCTTGCATTGTCCTTGATTGTCAGCTTCAATGGCGTCAGTGCAGCTCTTTACACTCTAATGGCCAATGCATTAGACCCTAATGATGCAAGCTTGTACCTTCTCCTAAATGCATTGGTTCCTCTCATAATCTCTGTCGTTACCTTCTTCCCAATCCTTCATCAGCCACCGGCTCAGCTGTCGTCTGCCGATGCCACCCGCAATGACTCCCTCACTTTCATCTGCTTATACATCACCGCAGTTATCACAGGCCTGTATCTAATCACCTTCAATTCCACGCCTTCCAGCAAGTACGGAGCCCAACTTTTGTTAGCTGGAGCTTTTGCCCTGTTACTAGTTCCTCTTTGTCTGCCAGGAATTTGGAGTACTCACAAGTGGTTGTTTCGGATCGCATCCACTGGCTTGAGAAGTGTTCTTCACTTTAGATTCAACCTTGTCAATCATGAGCTTCAGCAGGAGCTCATCCCTATTGAAACAGAAAGAAGTGGCACGAAGGTCATCACCCCTTTTGATTATAAGGAGAAGGAAAGCATTTCATGGAAGATGATGGGGAAAGAGAATTTGACAGTTCTTGAAGAAGAGCATTCAGCTAAAATGCTCGTACGACGCTTGGAGTTTTGGCTCTATTTTGTTGCATACTTTTGTGGTGGGACAATTGGGTTGGTTTACAGCAACAATCTTGGGCAGATCTCGCAGTCACTAGGATACAGTTCCTTAACAAGCTCACTTGTAACACTCTACTCTTCTTGCTCCTTCTTTGGGCGGCTAATCTCAGCAGGCCCAGATTTCATGCGTGAGTATGTAGTAGCAACAAACGGGTCAATCCTGCATATATATTCATTTTCCATGAATGATTTGACTTCAGATAACTCATAACTTTTCCATTTGCATTAGAAAGTATGGATTCATAATCTTTCTGCTAAACTTAGTGTAGCAAATGCAAAATTGGTTCTTCTGTTCAGATCAGAAACTAGGAAGTATGGGTTCATATTCCTTCTTCTAGATTTCATGTACTGACATCAAAAGGTTCATATCTAGATGCAGGATTCATCAGTCATTGAAACTTTCCATTTTGCATTAGAAATTTTGGCATCATATTCAATTCTTGTAAACTTCCAAAACACAATCAGTAATCAACAACAGAGAAAAACAATGCTAGTGAACTGATTAAACTTCGTACCTGTGCAGAAAGGTGCACTTCGCTAGAACTGGATGGCTAGCCCTAGCACTAGTGCCAACTCCAATCGCCTTCATTTTACTCGCTGCATCAGGCAGCGAGATCGCATTACAAATTGGCACTGGCTTGATCGGTCTAAGCTCGGGATTCATGTTCTCAGCCTCGGTCTCAATCACATCAGAGTTATTCGGACCAAACAGCTCAGGCGTCAATCACAACATCCTCATCACCAACATCCCCCTCGGCTCGTTCCTCTACGGCGTCCTGGCGGCAGTTTCATACGACTCAACTGCCGGAAGTTCAAACCAAACTGCGGCGGCGCTTGGGGACGTGGTGGTGTGCATCGGCCGGAAATGCTATCTACAAACCTTCATATGGTGGGCTTGCATCTCCATCATCGGCCTCGCTTCTTGCTTCTTGCTTTTCCGACGGACGAAACCGGCATACGACCGCCATTACGAGAGTAACCCAAACAAGACATAG

mRNA sequence

ATGAAAATAAAACACAGAAAACCTCAAAATTTGCCCCTTCTTCCCGTCACAAAACAGAGAAGGATGATGAACCAACCTGGGAAATGGGTGGCGCTGGTGGCGGCCATATGGATTCAGGCATTTACAGGCACGAATTTTGATTTCCCCTCTTATTCTTCCAATCTGAAGGCGGCTTTGGGGATGTCGCAGGTAGAGCTTAACTATCTGGCGGTGGCTTCTGACGTCGGTAAGGCCTTTGGGTGGTGCTCTGGCATCGCTCTCTTGTATTTTCCTTTGTGGGTGGTGATGCTCATGGCTGCTTCCATGGGGTTTCTTGGCTATGGCCTTCAATGGCTTCTCCTTCAAGGGATTGTATCTCTGCCTTACTCCATGGTATATCTTCTCTGCTTGTTGGCTGGATGTAGTATTTGCTGGTTTAATACAATCTGCTATGTCTCCTGCATCCAAAAGTTTCCAACCAATCGCGCGCTTGCATTGTCCTTGATTGTCAGCTTCAATGGCGTCAGTGCAGCTCTTTACACTCTAATGGCCAATGCATTAGACCCTAATGATGCAAGCTTGTACCTTCTCCTAAATGCATTGGTTCCTCTCATAATCTCTGTCGTTACCTTCTTCCCAATCCTTCATCAGCCACCGGCTCAGCTGTCGTCTGCCGATGCCACCCGCAATGACTCCCTCACTTTCATCTGCTTATACATCACCGCAGTTATCACAGGCCTGTATCTAATCACCTTCAATTCCACGCCTTCCAGCAAGTACGGAGCCCAACTTTTGTTAGCTGGAGCTTTTGCCCTGTTACTAGTTCCTCTTTGTCTGCCAGGAATTTGGAGTACTCACAAGTGGTTGTTTCGGATCGCATCCACTGGCTTGAGAAGTGTTCTTCACTTTAGATTCAACCTTGTCAATCATGAGCTTCAGCAGGAGCTCATCCCTATTGAAACAGAAAGAAGTGGCACGAAGGTCATCACCCCTTTTGATTATAAGGAGAAGGAAAGCATTTCATGGAAGATGATGGGGAAAGAGAATTTGACAGTTCTTGAAGAAGAGCATTCAGCTAAAATGCTCGTACGACGCTTGGAGTTTTGGCTCTATTTTGTTGCATACTTTTGTGGTGGGACAATTGGGTTGGTTTACAGCAACAATCTTGGGCAGATCTCGCAGTCACTAGGATACAGTTCCTTAACAAGCTCACTTGTAACACTCTACTCTTCTTGCTCCTTCTTTGGGCGGCTAATCTCAGCAGGCCCAGATTTCATGCGTGAAAAGGTGCACTTCGCTAGAACTGGATGGCTAGCCCTAGCACTAGTGCCAACTCCAATCGCCTTCATTTTACTCGCTGCATCAGGCAGCGAGATCGCATTACAAATTGGCACTGGCTTGATCGGTCTAAGCTCGGGATTCATGTTCTCAGCCTCGGTCTCAATCACATCAGAGTTATTCGGACCAAACAGCTCAGGCGTCAATCACAACATCCTCATCACCAACATCCCCCTCGGCTCGTTCCTCTACGGCGTCCTGGCGGCAGTTTCATACGACTCAACTGCCGGAAGTTCAAACCAAACTGCGGCGGCGCTTGGGGACGTGGTGGTGTGCATCGGCCGGAAATGCTATCTACAAACCTTCATATGGTGGGCTTGCATCTCCATCATCGGCCTCGCTTCTTGCTTCTTGCTTTTCCGACGGACGAAACCGGCATACGACCGCCATTACGAGAGTAACCCAAACAAGACATAG

Coding sequence (CDS)

ATGAAAATAAAACACAGAAAACCTCAAAATTTGCCCCTTCTTCCCGTCACAAAACAGAGAAGGATGATGAACCAACCTGGGAAATGGGTGGCGCTGGTGGCGGCCATATGGATTCAGGCATTTACAGGCACGAATTTTGATTTCCCCTCTTATTCTTCCAATCTGAAGGCGGCTTTGGGGATGTCGCAGGTAGAGCTTAACTATCTGGCGGTGGCTTCTGACGTCGGTAAGGCCTTTGGGTGGTGCTCTGGCATCGCTCTCTTGTATTTTCCTTTGTGGGTGGTGATGCTCATGGCTGCTTCCATGGGGTTTCTTGGCTATGGCCTTCAATGGCTTCTCCTTCAAGGGATTGTATCTCTGCCTTACTCCATGGTATATCTTCTCTGCTTGTTGGCTGGATGTAGTATTTGCTGGTTTAATACAATCTGCTATGTCTCCTGCATCCAAAAGTTTCCAACCAATCGCGCGCTTGCATTGTCCTTGATTGTCAGCTTCAATGGCGTCAGTGCAGCTCTTTACACTCTAATGGCCAATGCATTAGACCCTAATGATGCAAGCTTGTACCTTCTCCTAAATGCATTGGTTCCTCTCATAATCTCTGTCGTTACCTTCTTCCCAATCCTTCATCAGCCACCGGCTCAGCTGTCGTCTGCCGATGCCACCCGCAATGACTCCCTCACTTTCATCTGCTTATACATCACCGCAGTTATCACAGGCCTGTATCTAATCACCTTCAATTCCACGCCTTCCAGCAAGTACGGAGCCCAACTTTTGTTAGCTGGAGCTTTTGCCCTGTTACTAGTTCCTCTTTGTCTGCCAGGAATTTGGAGTACTCACAAGTGGTTGTTTCGGATCGCATCCACTGGCTTGAGAAGTGTTCTTCACTTTAGATTCAACCTTGTCAATCATGAGCTTCAGCAGGAGCTCATCCCTATTGAAACAGAAAGAAGTGGCACGAAGGTCATCACCCCTTTTGATTATAAGGAGAAGGAAAGCATTTCATGGAAGATGATGGGGAAAGAGAATTTGACAGTTCTTGAAGAAGAGCATTCAGCTAAAATGCTCGTACGACGCTTGGAGTTTTGGCTCTATTTTGTTGCATACTTTTGTGGTGGGACAATTGGGTTGGTTTACAGCAACAATCTTGGGCAGATCTCGCAGTCACTAGGATACAGTTCCTTAACAAGCTCACTTGTAACACTCTACTCTTCTTGCTCCTTCTTTGGGCGGCTAATCTCAGCAGGCCCAGATTTCATGCGTGAAAAGGTGCACTTCGCTAGAACTGGATGGCTAGCCCTAGCACTAGTGCCAACTCCAATCGCCTTCATTTTACTCGCTGCATCAGGCAGCGAGATCGCATTACAAATTGGCACTGGCTTGATCGGTCTAAGCTCGGGATTCATGTTCTCAGCCTCGGTCTCAATCACATCAGAGTTATTCGGACCAAACAGCTCAGGCGTCAATCACAACATCCTCATCACCAACATCCCCCTCGGCTCGTTCCTCTACGGCGTCCTGGCGGCAGTTTCATACGACTCAACTGCCGGAAGTTCAAACCAAACTGCGGCGGCGCTTGGGGACGTGGTGGTGTGCATCGGCCGGAAATGCTATCTACAAACCTTCATATGGTGGGCTTGCATCTCCATCATCGGCCTCGCTTCTTGCTTCTTGCTTTTCCGACGGACGAAACCGGCATACGACCGCCATTACGAGAGTAACCCAAACAAGACATAG

Protein sequence

MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALGMSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSLPYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANALDPNDASLYLLLNALVPLIISVVTFFPILHQPPAQLSSADATRNDSLTFICLYITAVITGLYLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLRSVLHFRFNLVNHELQQELIPIETERSGTKVITPFDYKEKESISWKMMGKENLTVLEEEHSAKMLVRRLEFWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMREKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGRKCYLQTFIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT

Homology

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BLAST of Csor.00g235450 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 3.6e-156
Identity = 316/574 (55.05%), Postives = 402/574 (70.03%), Query Frame = 0


Query: 5   HRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALGMSQV 64
           HRK  +LP L     R M  +  KW  LVAAIWIQA TGTNFDF +YSS+LK+ LG+SQV
Sbjct: 27  HRK--HLPPL-----RTMFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQV 86

Query: 65  ELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSLPYSM 124
            LNYLAVASD+GKAFGW SGIAL YFPL VV+  AA+MGF+GYG+QWL++  I++LPYS+
Sbjct: 87  RLNYLAVASDLGKAFGWSSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLPYSL 146

Query: 125 VYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANALDPND 184
           V+L CLLAG SICWFNT C++ CI+ FP NRALALSL VSFNG+SAALY+L  NA++P+ 
Sbjct: 147 VFLCCLLAGLSICWFNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSS 206

Query: 185 ASLYLLLNALVPLIISVVTFFPILHQPPAQLS-SADATRNDSLTFICLYITAVITGLYLI 244
           ++LYLLLN+LVPL++S    +P+L +P    +   D+ R+DS  F  L + AVIT  +L+
Sbjct: 207 SNLYLLLNSLVPLVVSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLL 266

Query: 245 TFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLRSVLHFRFNLVNH 304
             +S+ SS   A+L   GA  LL+ PLC P +     +   + +  L           NH
Sbjct: 267 LSSSSTSS---ARLNFIGAVVLLVFPLCAPLLVYARDYFLPVINARL-----------NH 326

Query: 305 ELQQELIPIETERSGTKVITPFDYK-EKESIS----WKMMG--KENLTV-LEEEHSAKML 364
                      E SG  ++   + K +K S+S    ++ MG  KE  TV L +EHS ++L
Sbjct: 327 -----------ESSGYVMLNIDELKNQKTSVSSKTGYEHMGTAKEGNTVRLGDEHSFRLL 386

Query: 365 VRRLEFWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAG 424
           + RLEFWLY++AYFCGGTIGLVYSNNLGQI+QSLG +S T  LVT+YSS SFFGRL+SA 
Sbjct: 387 ISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNSTT--LVTIYSSFSFFGRLLSAA 446

Query: 425 PDFMREKVHFARTGWLALALVPTPIAFILLAASGS-EIALQIGTGLIGLSSGFMFSASVS 484
           PDFM ++    RTGW A+AL+PTPIAF LLA S S + ALQ  T LIGLSSGF+F+A+VS
Sbjct: 447 PDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQTATALIGLSSGFIFAAAVS 506

Query: 485 ITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGR 544
           ITS+LFGPNS GVNHNILITNIP+GS LYG +AA  Y+  A +S      + D +VCIGR
Sbjct: 507 ITSDLFGPNSVGVNHNILITNIPIGSLLYGYIAASIYE--ANASPDITPIVSDSIVCIGR 564

Query: 545 KCYLQTFIWWACISIIGLASCFLLFRRTKPAYDR 569
            CY +TF++W C+SI+G+ S   L+ RTKP Y R
Sbjct: 567 DCYFKTFVFWGCLSILGVVSSLSLYIRTKPVYHR 564

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BLAST of Csor.00g235450 vs. ExPASy Swiss-Prot
Match: A0LNN5 (L-lactate transporter OS=Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) OX=335543 GN=Sfum_3364 PE=1 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 1.3e-04
Identity = 37/142 (26.06%), Postives = 65/142 (45.77%), Query Frame = 0


Query: 360 EFWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFM 419
           +FWL ++AYFCG   GL+   +L    +  G +++ ++     SS +F          + 
Sbjct: 229 KFWLLYLAYFCGSFAGLMVIGHLAGFGRDAGLTAMAAA--GAVSSLAFSNAATRILSGWF 288

Query: 420 REKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSEL 479
            +K+   R  + AL  + T     +    GS + L I   +IG + G MF+   +   + 
Sbjct: 289 VDKIGI-RVYFAALFALQTAAMIAIFQLGGSVVGLSIVAIVIGWNYGAMFTLFPATCLQF 348

Query: 480 FGPNSSGVNHNILITNIPLGSF 502
           +GP + G N+ +L T   L  F
Sbjct: 349 YGPTAQGSNYGLLFTACGLAGF 367

[Close]

BLAST of Csor.00g235450 vs. NCBI nr
Match: KAG6593740.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1132 bits (2927), Expect = 0.0
Identity = 577/577 (100.00%), Postives = 577/577 (100.00%), Query Frame = 0


Query: 1   MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG 60
           MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG
Sbjct: 1   MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG 60

Query: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL 120
           MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL
Sbjct: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL 120

Query: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL 180
           PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL
Sbjct: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL 180

Query: 181 DPNDASLYLLLNALVPLIISVVTFFPILHQPPAQLSSADATRNDSLTFICLYITAVITGL 240
           DPNDASLYLLLNALVPLIISVVTFFPILHQPPAQLSSADATRNDSLTFICLYITAVITGL
Sbjct: 181 DPNDASLYLLLNALVPLIISVVTFFPILHQPPAQLSSADATRNDSLTFICLYITAVITGL 240

Query: 241 YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLRSVLHFRFNL 300
           YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLRSVLHFRFNL
Sbjct: 241 YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLRSVLHFRFNL 300

Query: 301 VNHELQQELIPIETERSGTKVITPFDYKEKESISWKMMGKENLTVLEEEHSAKMLVRRLE 360
           VNHELQQELIPIETERSGTKVITPFDYKEKESISWKMMGKENLTVLEEEHSAKMLVRRLE
Sbjct: 301 VNHELQQELIPIETERSGTKVITPFDYKEKESISWKMMGKENLTVLEEEHSAKMLVRRLE 360

Query: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420
           FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR
Sbjct: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420

Query: 421 EKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480
           EKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF
Sbjct: 421 EKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480

Query: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGRKCYLQT 540
           GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGRKCYLQT
Sbjct: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGRKCYLQT 540

Query: 541 FIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT 577
           FIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT
Sbjct: 541 FIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT 577

[Close]

BLAST of Csor.00g235450 vs. NCBI nr
Match: KAG7026076.1 (Protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1123 bits (2906), Expect = 0.0
Identity = 572/577 (99.13%), Postives = 574/577 (99.48%), Query Frame = 0


Query: 1   MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG 60
           MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALV AIWIQAFTGTNFDFPSYSSNLKAALG
Sbjct: 1   MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVVAIWIQAFTGTNFDFPSYSSNLKAALG 60

Query: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL 120
           MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL
Sbjct: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL 120

Query: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL 180
           PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL
Sbjct: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL 180

Query: 181 DPNDASLYLLLNALVPLIISVVTFFPILHQPPAQLSSADATRNDSLTFICLYITAVITGL 240
           DPNDASLYLLLNALVPLIISVVTFFPILHQPPAQ SSADATRNDSLTFICLYITAV+TGL
Sbjct: 181 DPNDASLYLLLNALVPLIISVVTFFPILHQPPAQPSSADATRNDSLTFICLYITAVVTGL 240

Query: 241 YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLRSVLHFRFNL 300
           YLITFNSTPSSKYGAQLLLAGAF+LLLVPLCLPGIWSTHKWLFRIASTGL SVLHFRFNL
Sbjct: 241 YLITFNSTPSSKYGAQLLLAGAFSLLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300

Query: 301 VNHELQQELIPIETERSGTKVITPFDYKEKESISWKMMGKENLTVLEEEHSAKMLVRRLE 360
           VNHELQQELIPIETERSGTKVITPFDYKEKESISWKMMGKENLTVLEEEHSAKMLVRRLE
Sbjct: 301 VNHELQQELIPIETERSGTKVITPFDYKEKESISWKMMGKENLTVLEEEHSAKMLVRRLE 360

Query: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420
           FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR
Sbjct: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420

Query: 421 EKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480
           EKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF
Sbjct: 421 EKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480

Query: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGRKCYLQT 540
           GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGRKCYLQT
Sbjct: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGRKCYLQT 540

Query: 541 FIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT 577
           FIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT
Sbjct: 541 FIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT 577

[Close]

BLAST of Csor.00g235450 vs. NCBI nr
Match: XP_022964108.1 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1117 bits (2890), Expect = 0.0
Identity = 571/577 (98.96%), Postives = 573/577 (99.31%), Query Frame = 0


Query: 1   MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG 60
           MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG
Sbjct: 1   MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG 60

Query: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL 120
           MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL
Sbjct: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL 120

Query: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL 180
           PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL
Sbjct: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL 180

Query: 181 DPNDASLYLLLNALVPLIISVVTFFPILHQPPAQLSSADATRNDSLTFICLYITAVITGL 240
           DPNDASLYLLLNALVPLIISVVTF PILHQPPAQ SSADATRNDSLTFICLYITAVITGL
Sbjct: 181 DPNDASLYLLLNALVPLIISVVTFLPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240

Query: 241 YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLRSVLHFRFNL 300
           YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGL SVLHFRFNL
Sbjct: 241 YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300

Query: 301 VNHELQQELIPIETERSGTKVITPFDYKEKESISWKMMGKENLTVLEEEHSAKMLVRRLE 360
           VNHELQQELIPIETERSGTKVITPFDYKEKESIS KMMGKENLTVLEEEHSAKML+RRL+
Sbjct: 301 VNHELQQELIPIETERSGTKVITPFDYKEKESISRKMMGKENLTVLEEEHSAKMLIRRLD 360

Query: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420
           FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR
Sbjct: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420

Query: 421 EKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480
           EKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF
Sbjct: 421 EKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480

Query: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGRKCYLQT 540
           GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGRKCYLQT
Sbjct: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGRKCYLQT 540

Query: 541 FIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT 577
           FIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT
Sbjct: 541 FIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT 577

[Close]

BLAST of Csor.00g235450 vs. NCBI nr
Match: XP_022964107.1 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1110 bits (2872), Expect = 0.0
Identity = 571/584 (97.77%), Postives = 573/584 (98.12%), Query Frame = 0


Query: 1   MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG 60
           MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG
Sbjct: 1   MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG 60

Query: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL 120
           MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL
Sbjct: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL 120

Query: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL 180
           PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL
Sbjct: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL 180

Query: 181 DPNDASLYLLLNALVPLIISVVTFFPILHQPPAQLSSADATRNDSLTFICLYITAVITGL 240
           DPNDASLYLLLNALVPLIISVVTF PILHQPPAQ SSADATRNDSLTFICLYITAVITGL
Sbjct: 181 DPNDASLYLLLNALVPLIISVVTFLPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240

Query: 241 YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLRSVLHFRFNL 300
           YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGL SVLHFRFNL
Sbjct: 241 YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300

Query: 301 VNHELQQELIPIETERSGTKVITPFDYKEKESISWKMMGKENLTVLEEEHSAKMLVRRLE 360
           VNHELQQELIPIETERSGTKVITPFDYKEKESIS KMMGKENLTVLEEEHSAKML+RRL+
Sbjct: 301 VNHELQQELIPIETERSGTKVITPFDYKEKESISRKMMGKENLTVLEEEHSAKMLIRRLD 360

Query: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420
           FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR
Sbjct: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420

Query: 421 E-------KVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASV 480
           E       KVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASV
Sbjct: 421 EYVVATNGKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASV 480

Query: 481 SITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIG 540
           SITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIG
Sbjct: 481 SITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIG 540

Query: 541 RKCYLQTFIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT 577
           RKCYLQTFIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT
Sbjct: 541 RKCYLQTFIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT 584

[Close]

BLAST of Csor.00g235450 vs. NCBI nr
Match: XP_023515123.1 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1093 bits (2828), Expect = 0.0
Identity = 560/577 (97.05%), Postives = 567/577 (98.27%), Query Frame = 0


Query: 1   MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG 60
           MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG
Sbjct: 1   MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG 60

Query: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL 120
           MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL
Sbjct: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL 120

Query: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL 180
           PYSMVYLLCLLAGCSICWFNTICYVSCIQKFP NRALALSLIVSFNGVSAALYTLMANA+
Sbjct: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMANAV 180

Query: 181 DPNDASLYLLLNALVPLIISVVTFFPILHQPPAQLSSADATRNDSLTFICLYITAVITGL 240
           DPNDASLYLLLNALVPLIISVVTF PILHQPPAQ SS+DATRNDS TFICLYITAVITGL
Sbjct: 181 DPNDASLYLLLNALVPLIISVVTFLPILHQPPAQPSSSDATRNDSHTFICLYITAVITGL 240

Query: 241 YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLRSVLHFRFNL 300
           YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGL SVLHFRFNL
Sbjct: 241 YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300

Query: 301 VNHELQQELIPIETERSGTKVITPFDYKEKESISWKMMGKENLTVLEEEHSAKMLVRRLE 360
           VNHELQQELI +ETERSGT VITPFDYKEKESIS KMMGKENLTVLEEEHSAKMLVRRL+
Sbjct: 301 VNHELQQELINLETERSGTNVITPFDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD 360

Query: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420
           FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR
Sbjct: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420

Query: 421 EKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480
           EKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF
Sbjct: 421 EKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480

Query: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGRKCYLQT 540
           GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSS+QTAAALGDVVVCIGRKCYLQT
Sbjct: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSDQTAAALGDVVVCIGRKCYLQT 540

Query: 541 FIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT 577
           FIWWACISI+GLAS FLLFRRTKPAYDR YESNP+KT
Sbjct: 541 FIWWACISIVGLASSFLLFRRTKPAYDRQYESNPSKT 577

[Close]

BLAST of Csor.00g235450 vs. ExPASy TrEMBL
Match: A0A6J1HJV4 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111464237 PE=4 SV=1)

HSP 1 Score: 1117 bits (2890), Expect = 0.0
Identity = 571/577 (98.96%), Postives = 573/577 (99.31%), Query Frame = 0


Query: 1   MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG 60
           MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG
Sbjct: 1   MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG 60

Query: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL 120
           MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL
Sbjct: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL 120

Query: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL 180
           PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL
Sbjct: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL 180

Query: 181 DPNDASLYLLLNALVPLIISVVTFFPILHQPPAQLSSADATRNDSLTFICLYITAVITGL 240
           DPNDASLYLLLNALVPLIISVVTF PILHQPPAQ SSADATRNDSLTFICLYITAVITGL
Sbjct: 181 DPNDASLYLLLNALVPLIISVVTFLPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240

Query: 241 YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLRSVLHFRFNL 300
           YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGL SVLHFRFNL
Sbjct: 241 YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300

Query: 301 VNHELQQELIPIETERSGTKVITPFDYKEKESISWKMMGKENLTVLEEEHSAKMLVRRLE 360
           VNHELQQELIPIETERSGTKVITPFDYKEKESIS KMMGKENLTVLEEEHSAKML+RRL+
Sbjct: 301 VNHELQQELIPIETERSGTKVITPFDYKEKESISRKMMGKENLTVLEEEHSAKMLIRRLD 360

Query: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420
           FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR
Sbjct: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420

Query: 421 EKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480
           EKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF
Sbjct: 421 EKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480

Query: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGRKCYLQT 540
           GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGRKCYLQT
Sbjct: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGRKCYLQT 540

Query: 541 FIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT 577
           FIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT
Sbjct: 541 FIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT 577

[Close]

BLAST of Csor.00g235450 vs. ExPASy TrEMBL
Match: A0A6J1HGX4 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464237 PE=4 SV=1)

HSP 1 Score: 1110 bits (2872), Expect = 0.0
Identity = 571/584 (97.77%), Postives = 573/584 (98.12%), Query Frame = 0


Query: 1   MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG 60
           MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG
Sbjct: 1   MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG 60

Query: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL 120
           MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL
Sbjct: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL 120

Query: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL 180
           PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL
Sbjct: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL 180

Query: 181 DPNDASLYLLLNALVPLIISVVTFFPILHQPPAQLSSADATRNDSLTFICLYITAVITGL 240
           DPNDASLYLLLNALVPLIISVVTF PILHQPPAQ SSADATRNDSLTFICLYITAVITGL
Sbjct: 181 DPNDASLYLLLNALVPLIISVVTFLPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240

Query: 241 YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLRSVLHFRFNL 300
           YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGL SVLHFRFNL
Sbjct: 241 YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300

Query: 301 VNHELQQELIPIETERSGTKVITPFDYKEKESISWKMMGKENLTVLEEEHSAKMLVRRLE 360
           VNHELQQELIPIETERSGTKVITPFDYKEKESIS KMMGKENLTVLEEEHSAKML+RRL+
Sbjct: 301 VNHELQQELIPIETERSGTKVITPFDYKEKESISRKMMGKENLTVLEEEHSAKMLIRRLD 360

Query: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420
           FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR
Sbjct: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420

Query: 421 E-------KVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASV 480
           E       KVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASV
Sbjct: 421 EYVVATNGKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASV 480

Query: 481 SITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIG 540
           SITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIG
Sbjct: 481 SITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIG 540

Query: 541 RKCYLQTFIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT 577
           RKCYLQTFIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT
Sbjct: 541 RKCYLQTFIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT 584

[Close]

BLAST of Csor.00g235450 vs. ExPASy TrEMBL
Match: A0A6J1KEQ9 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111494334 PE=4 SV=1)

HSP 1 Score: 1091 bits (2821), Expect = 0.0
Identity = 556/577 (96.36%), Postives = 564/577 (97.75%), Query Frame = 0


Query: 1   MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG 60
           MKIKHRKPQN P LPVTKQRRMMNQPGKWVAL+AAIWIQAFTGTNFDFPSYSSNLKAALG
Sbjct: 1   MKIKHRKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALG 60

Query: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL 120
           MSQVELNYLAVASDVGKAFGWCSGIALLYFP WVVMLMAASMGFLGYGLQWLLLQGIVSL
Sbjct: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSL 120

Query: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL 180
           PYSMVYLLCLLAGCSICWFNTICYVSCIQKFP NRALALSLIVSFNGVSAALYTLM NA+
Sbjct: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAV 180

Query: 181 DPNDASLYLLLNALVPLIISVVTFFPILHQPPAQLSSADATRNDSLTFICLYITAVITGL 240
           DPNDASLYLLLNALVPLIISVV FFPILHQPPAQ SSADATRNDSLTFICLYITAVITGL
Sbjct: 181 DPNDASLYLLLNALVPLIISVVAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240

Query: 241 YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLRSVLHFRFNL 300
           YLITFNSTPSSKYGAQ+LLAGAFALLLVPLCLPGIWSTHKWLFRIASTGL SVLHFRFNL
Sbjct: 241 YLITFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300

Query: 301 VNHELQQELIPIETERSGTKVITPFDYKEKESISWKMMGKENLTVLEEEHSAKMLVRRLE 360
           VNHELQQELI +E+ERSGTKVITP DYKEKESIS KMMGKENLTVLEEEHSAKMLVRRL+
Sbjct: 301 VNHELQQELINVESERSGTKVITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD 360

Query: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420
           FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR
Sbjct: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420

Query: 421 EKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480
           EKVHFARTGWLALALVP PIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF
Sbjct: 421 EKVHFARTGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480

Query: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGRKCYLQT 540
           GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAA LGDVVVCIGRKCYLQT
Sbjct: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIGRKCYLQT 540

Query: 541 FIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT 577
           FIWWACISI+GLASCFLLFRRTKPAYDRHYESNP+KT
Sbjct: 541 FIWWACISIVGLASCFLLFRRTKPAYDRHYESNPSKT 577

[Close]

BLAST of Csor.00g235450 vs. ExPASy TrEMBL
Match: A0A6J1KH74 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111494334 PE=4 SV=1)

HSP 1 Score: 1084 bits (2803), Expect = 0.0
Identity = 556/584 (95.21%), Postives = 564/584 (96.58%), Query Frame = 0


Query: 1   MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG 60
           MKIKHRKPQN P LPVTKQRRMMNQPGKWVAL+AAIWIQAFTGTNFDFPSYSSNLKAALG
Sbjct: 1   MKIKHRKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALG 60

Query: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL 120
           MSQVELNYLAVASDVGKAFGWCSGIALLYFP WVVMLMAASMGFLGYGLQWLLLQGIVSL
Sbjct: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSL 120

Query: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL 180
           PYSMVYLLCLLAGCSICWFNTICYVSCIQKFP NRALALSLIVSFNGVSAALYTLM NA+
Sbjct: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAV 180

Query: 181 DPNDASLYLLLNALVPLIISVVTFFPILHQPPAQLSSADATRNDSLTFICLYITAVITGL 240
           DPNDASLYLLLNALVPLIISVV FFPILHQPPAQ SSADATRNDSLTFICLYITAVITGL
Sbjct: 181 DPNDASLYLLLNALVPLIISVVAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240

Query: 241 YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLRSVLHFRFNL 300
           YLITFNSTPSSKYGAQ+LLAGAFALLLVPLCLPGIWSTHKWLFRIASTGL SVLHFRFNL
Sbjct: 241 YLITFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300

Query: 301 VNHELQQELIPIETERSGTKVITPFDYKEKESISWKMMGKENLTVLEEEHSAKMLVRRLE 360
           VNHELQQELI +E+ERSGTKVITP DYKEKESIS KMMGKENLTVLEEEHSAKMLVRRL+
Sbjct: 301 VNHELQQELINVESERSGTKVITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD 360

Query: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420
           FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR
Sbjct: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420

Query: 421 E-------KVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASV 480
           E       KVHFARTGWLALALVP PIAFILLAASGSEIALQIGTGLIGLSSGFMFSASV
Sbjct: 421 EYVVAANGKVHFARTGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASV 480

Query: 481 SITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIG 540
           SITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAA LGDVVVCIG
Sbjct: 481 SITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIG 540

Query: 541 RKCYLQTFIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT 577
           RKCYLQTFIWWACISI+GLASCFLLFRRTKPAYDRHYESNP+KT
Sbjct: 541 RKCYLQTFIWWACISIVGLASCFLLFRRTKPAYDRHYESNPSKT 584

[Close]

BLAST of Csor.00g235450 vs. ExPASy TrEMBL
Match: A0A1S3CCZ1 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103499000 PE=4 SV=1)

HSP 1 Score: 942 bits (2435), Expect = 0.0
Identity = 480/576 (83.33%), Postives = 519/576 (90.10%), Query Frame = 0


Query: 1   MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG 60
           MKIKHRK Q +PLLP  KQ+RMMNQPGKWV LVAAIWIQAF GTNFDFPSYSS+LKAALG
Sbjct: 1   MKIKHRKTQQIPLLPFKKQKRMMNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALG 60

Query: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL 120
           MSQVELNYLAVASD+GKAFGWCSG+ALLYFPLWVVM MAASMGFLGYGLQWLLLQ I+SL
Sbjct: 61  MSQVELNYLAVASDLGKAFGWCSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRIISL 120

Query: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL 180
           PYS+VYLLCL+AGCSICWFNT+CYVSCIQ FP NRALALSLIVSFNGVSAALYTL+ANA+
Sbjct: 121 PYSVVYLLCLMAGCSICWFNTVCYVSCIQNFPANRALALSLIVSFNGVSAALYTLIANAI 180

Query: 181 DPNDASLYLLLNALVPLIISVVTFFPILHQPPAQLSSADATRNDSLTFICLYITAVITGL 240
           DP DASLYL LNALVPLIISVV   P+LHQP  Q SSADA R+DSL FICLYITAVITGL
Sbjct: 181 DPGDASLYLFLNALVPLIISVVALLPMLHQPQVQPSSADAIRHDSLIFICLYITAVITGL 240

Query: 241 YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLRSVLHFRFNL 300
           YLITFNS PS+KYG+Q+LLAGAFALLLVPLCLPGI STH+WL RI ST L  ++H RF+L
Sbjct: 241 YLITFNSMPSNKYGSQILLAGAFALLLVPLCLPGILSTHRWLVRIISTSLNCLIHSRFSL 300

Query: 301 VNHELQQELIPIETERSGTKVITPFDYKEKESISWKMMGKENLTVLEEEHSAKMLVRRLE 360
            +HEL QEL  I +ER+    I PF+ KEKESIS K+M KENL VLEEEHSAKML+R+L+
Sbjct: 301 ADHELHQELFTIGSERNSMMDIIPFESKEKESISRKVMEKENLVVLEEEHSAKMLMRQLD 360

Query: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420
           FWLY+ AYFCGGTIGLVYSNNLGQI+QSLGYSS TSSLVTLYSSCSFFGRLISA PDFMR
Sbjct: 361 FWLYYAAYFCGGTIGLVYSNNLGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMR 420

Query: 421 EKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480
           EK HFARTGWLA+ALVPTPIAF+LLAASGS+IALQ GT LIGLSSGF+FSASVSITSELF
Sbjct: 421 EKGHFARTGWLAIALVPTPIAFVLLAASGSKIALQAGTSLIGLSSGFIFSASVSITSELF 480

Query: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGRKCYLQT 540
           GPNSSGVNHNILITNIPLGSFLYGVLAAV+YDS AGSS+QTAA LGD VVCIG+ CYL T
Sbjct: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVAYDSNAGSSHQTAA-LGDAVVCIGQNCYLMT 540

Query: 541 FIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNK 576
           F+WWACISI GLAS FLLFRRTK AYDRHYESN +K
Sbjct: 541 FVWWACISIFGLASSFLLFRRTKSAYDRHYESNLSK 575

[Close]

BLAST of Csor.00g235450 vs. TAIR 10
Match: AT5G45275.1 (Major facilitator superfamily protein )

HSP 1 Score: 611.7 bits (1576), Expect = 6.1e-175
Identity = 326/562 (58.01%), Postives = 402/562 (71.53%), Query Frame = 0


Query: 22  MMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALGMSQVELNYLAVASDVGKAFGW 81
           M  Q  KW+ LVA IWIQAFTGTNFDF +YSSNLK+ LG+SQV+LNYLAVASD+GK FGW
Sbjct: 1   MAGQSRKWMILVATIWIQAFTGTNFDFSTYSSNLKSVLGISQVQLNYLAVASDLGKVFGW 60

Query: 82  CSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSLPYSMVYLLCLLAGCSICWFNT 141
            SG+ALLYFPLW V+  AA MGF+GYG+QWL++  ++SLPY +V+L CLLAG SICWFNT
Sbjct: 61  SSGLALLYFPLWTVLFAAAIMGFVGYGVQWLVITNVISLPYILVFLCCLLAGLSICWFNT 120

Query: 142 ICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANALDPNDASLYLLLNALVPLIISV 201
           +C+V CI+ FP NR+LALSL VSFNGVSAALYTL  NA++P    LYLLLNALVPL +S 
Sbjct: 121 VCFVLCIRNFPANRSLALSLTVSFNGVSAALYTLAYNAINPVSTELYLLLNALVPLFVSF 180

Query: 202 VTFFPILHQPPAQLSSADATRNDSLTFICLYITAVITGLYLITFNSTPSSKYGAQLLLAG 261
               PIL QPP +    D  R DSL F+ L I AV+ G+YL+ F S  S    A+LL  G
Sbjct: 181 AALIPILRQPPLEPLPPDGVRRDSLMFLLLNILAVLNGVYLLLFRSKTSDVTSARLLFGG 240

Query: 262 AFALLLVPLCLPGIWSTHKWL-------FRIASTGLRSV----LHFRFNLVNHELQQELI 321
           +  LL++PLCLPG+     W        FR+  +G   V    L     +V  E   E  
Sbjct: 241 SILLLILPLCLPGLVYARNWYLHNIHSSFRLEGSGFILVDVDELEMHKGMVTREASLEGY 300

Query: 322 PIETERSGTKVITP----FDYKEKESISWKMMGKENLTVLEEEHSAKMLVRRLEFWLYFV 381
            +  +     V TP    F   +      K++ +  L +L EEH    L+ R +FWLY++
Sbjct: 301 QLLNDDVVRAVNTPDQKSFIEDDDGCCCTKVITRNQLGMLGEEHPLSFLLCRSDFWLYYI 360

Query: 382 AYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMREKVHFA 441
           AYFCGGTIGLVYSNNLGQI+QSLG SS T++LVTLYSS SFFGRL+SA PD++R KV+FA
Sbjct: 361 AYFCGGTIGLVYSNNLGQIAQSLGQSSETTTLVTLYSSFSFFGRLLSATPDYIRAKVYFA 420

Query: 442 RTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELFGPNSSG 501
           RTGWLA+AL+PT IA  LLA+SGS  ALQ GT LIGLSSGF+F+A+VSITSELFGPNS G
Sbjct: 421 RTGWLAVALLPTTIALFLLASSGSLAALQAGTALIGLSSGFIFAAAVSITSELFGPNSVG 480

Query: 502 VNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGRKCYLQTFIWWAC 561
           VNHNILITNIP+GS +YG LAA+ Y+S + + ++T     + V+C+GR CYLQTF+WW C
Sbjct: 481 VNHNILITNIPIGSLVYGFLAALVYESHSVAGSKT-----ESVICMGRDCYLQTFMWWGC 540

Query: 562 ISIIGLASCFLLFRRTKPAYDR 569
           +S+IGLAS  +LF RT+ AY R
Sbjct: 541 LSVIGLASSVVLFLRTRRAYQR 557

[Close]

BLAST of Csor.00g235450 vs. TAIR 10
Match: AT4G19450.1 (Major facilitator superfamily protein )

HSP 1 Score: 606.3 bits (1562), Expect = 2.6e-173
Identity = 317/561 (56.51%), Postives = 396/561 (70.59%), Query Frame = 0


Query: 22  MMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALGMSQVELNYLAVASDVGKAFGW 81
           M  Q  KW+ LVA IWIQAFTGTNFDF +YSS+LK+ LG+SQV+LNYLAVASD+GK FGW
Sbjct: 1   MAGQSRKWMILVATIWIQAFTGTNFDFSAYSSDLKSVLGISQVQLNYLAVASDLGKVFGW 60

Query: 82  CSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSLPYSMVYLLCLLAGCSICWFNT 141
            SG+AL+YFPLW V+  AA MGF+GYG+QWL++   +SLPY MV+L CLLAG SICWFNT
Sbjct: 61  SSGLALMYFPLWTVLFAAAFMGFVGYGVQWLVITHFISLPYIMVFLCCLLAGLSICWFNT 120

Query: 142 ICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANALDPNDASLYLLLNALVPLIISV 201
           +C+V CI  FP NR+LALSL VSFNGVSAALYTL  NA++P    LYLLLNAL+PLI+S 
Sbjct: 121 VCFVLCISNFPANRSLALSLTVSFNGVSAALYTLAYNAINPTSPELYLLLNALIPLIVSF 180

Query: 202 VTFFPILHQPPAQLSSADATRNDSLTFICLYITAVITGLYLITFNSTPSSKYGAQLLLAG 261
               PIL QPP +    D  R DSL F+ L I A + G+YL+ F S  S    A+LL  G
Sbjct: 181 TAIIPILRQPPFEPLPPDGVRRDSLMFLLLNILAALNGVYLLLFGSNSSDLTSARLLFGG 240

Query: 262 AFALLLVPLCLPGIWSTHKWLFRIASTGLR-----------SVLHFRFNLVNHELQQE-- 321
           A  LL+ PLC+PG+     W  R   T  R             L     ++ HE  +E  
Sbjct: 241 AILLLVFPLCIPGLVIARNWYNRTIHTSFRLEGSGFILVDPDELELHKGMLAHEANREGY 300

Query: 322 -LIPIETERSGTKVITPFDYKEKESISWKMMGKENLTVLEEEHSAKMLVRRLEFWLYFVA 381
            L+  +  ++  K +   +    ES   K++ ++ L  L  EHS  +L+ R +FWLY++ 
Sbjct: 301 QLLSDDVVQNPVKSVAVEEEDSDESCCKKLITRDQLEGLGIEHSLSLLLTRSDFWLYYIT 360

Query: 382 YFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMREKVHFAR 441
           YFCGGTIGLVYSNNLGQI+QSLG SS T++LVTLYS+ SFFGRL+SA PD++R KV+FAR
Sbjct: 361 YFCGGTIGLVYSNNLGQIAQSLGQSSNTTTLVTLYSAFSFFGRLLSATPDYIRAKVYFAR 420

Query: 442 TGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELFGPNSSGV 501
           TGWLA+AL+PTP A  LLA+SG+  ALQ GT L+GLSSGF+F+A+VSITSELFGPNS GV
Sbjct: 421 TGWLAIALLPTPFALFLLASSGTASALQAGTALMGLSSGFIFAAAVSITSELFGPNSVGV 480

Query: 502 NHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGRKCYLQTFIWWACI 561
           NHNILITNIP+GS +YG LAA+ YDS   +   T +   + VVC+GR CY  TF+WW C+
Sbjct: 481 NHNILITNIPIGSLIYGFLAALVYDSHGFTG--TKSMTSESVVCMGRDCYYLTFVWWGCL 540

Query: 562 SIIGLASCFLLFRRTKPAYDR 569
           S++GL S  +LF RT+ AY R
Sbjct: 541 SLLGLGSSLVLFIRTRRAYQR 559

[Close]

BLAST of Csor.00g235450 vs. TAIR 10
Match: AT1G31470.1 (Major facilitator superfamily protein )

HSP 1 Score: 553.1 bits (1424), Expect = 2.6e-157
Identity = 316/574 (55.05%), Postives = 402/574 (70.03%), Query Frame = 0


Query: 5   HRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALGMSQV 64
           HRK  +LP L     R M  +  KW  LVAAIWIQA TGTNFDF +YSS+LK+ LG+SQV
Sbjct: 27  HRK--HLPPL-----RTMFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQV 86

Query: 65  ELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSLPYSM 124
            LNYLAVASD+GKAFGW SGIAL YFPL VV+  AA+MGF+GYG+QWL++  I++LPYS+
Sbjct: 87  RLNYLAVASDLGKAFGWSSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLPYSL 146

Query: 125 VYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANALDPND 184
           V+L CLLAG SICWFNT C++ CI+ FP NRALALSL VSFNG+SAALY+L  NA++P+ 
Sbjct: 147 VFLCCLLAGLSICWFNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSS 206

Query: 185 ASLYLLLNALVPLIISVVTFFPILHQPPAQLS-SADATRNDSLTFICLYITAVITGLYLI 244
           ++LYLLLN+LVPL++S    +P+L +P    +   D+ R+DS  F  L + AVIT  +L+
Sbjct: 207 SNLYLLLNSLVPLVVSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLL 266

Query: 245 TFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLRSVLHFRFNLVNH 304
             +S+ SS   A+L   GA  LL+ PLC P +     +   + +  L           NH
Sbjct: 267 LSSSSTSS---ARLNFIGAVVLLVFPLCAPLLVYARDYFLPVINARL-----------NH 326

Query: 305 ELQQELIPIETERSGTKVITPFDYK-EKESIS----WKMMG--KENLTV-LEEEHSAKML 364
                      E SG  ++   + K +K S+S    ++ MG  KE  TV L +EHS ++L
Sbjct: 327 -----------ESSGYVMLNIDELKNQKTSVSSKTGYEHMGTAKEGNTVRLGDEHSFRLL 386

Query: 365 VRRLEFWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAG 424
           + RLEFWLY++AYFCGGTIGLVYSNNLGQI+QSLG +S T  LVT+YSS SFFGRL+SA 
Sbjct: 387 ISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNSTT--LVTIYSSFSFFGRLLSAA 446

Query: 425 PDFMREKVHFARTGWLALALVPTPIAFILLAASGS-EIALQIGTGLIGLSSGFMFSASVS 484
           PDFM ++    RTGW A+AL+PTPIAF LLA S S + ALQ  T LIGLSSGF+F+A+VS
Sbjct: 447 PDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQTATALIGLSSGFIFAAAVS 506

Query: 485 ITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGR 544
           ITS+LFGPNS GVNHNILITNIP+GS LYG +AA  Y+  A +S      + D +VCIGR
Sbjct: 507 ITSDLFGPNSVGVNHNILITNIPIGSLLYGYIAASIYE--ANASPDITPIVSDSIVCIGR 564

Query: 545 KCYLQTFIWWACISIIGLASCFLLFRRTKPAYDR 569
            CY +TF++W C+SI+G+ S   L+ RTKP Y R
Sbjct: 567 DCYFKTFVFWGCLSILGVVSSLSLYIRTKPVYHR 564

[Close]

BLAST of Csor.00g235450 vs. TAIR 10
Match: AT3G01630.1 (Major facilitator superfamily protein )

HSP 1 Score: 485.7 bits (1249), Expect = 5.1e-137
Identity = 274/574 (47.74%), Postives = 377/574 (65.68%), Query Frame = 0


Query: 6   RKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALGMSQVE 65
           ++P + P  P  +   M+    +W  LVAAIWIQAFTGTNFDF +YSS++K+++G+SQ  
Sbjct: 3   KQPPSPPKPP--RVNSMLQWWRRWTVLVAAIWIQAFTGTNFDFSAYSSDMKSSMGVSQSR 62

Query: 66  LNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSLPYSMV 125
           LNY+AVASD+GKA GW SG A+ YFP+  V+  AA+MG +GYG+QWL +  ++ LPYS+V
Sbjct: 63  LNYMAVASDLGKALGWSSGFAIAYFPVSGVLFAAAAMGLVGYGVQWLSIADVIDLPYSLV 122

Query: 126 YLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANALDPNDA 185
            + C LAG SICWFNT+ ++ CI+ F  N +LALSL+VSFNG+SAALYTL   A+    +
Sbjct: 123 LVCCSLAGLSICWFNTVSFILCIRHFKANHSLALSLVVSFNGISAALYTLGHEAISGKSS 182

Query: 186 S---LYLLLNALVPLIISVVTFFPILHQPPAQLSSADATRNDSLTFICLYITAVITGLYL 245
           +   +YLLLN+L+PLI+SV+  +P+L  P +  +    T +++  F+   + A++T  YL
Sbjct: 183 ASSDIYLLLNSLIPLIVSVLALWPVLTNPNSSETDTTRTHDETRIFVVFNVLALVTCFYL 242

Query: 246 ITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLRSVLHFR---FN 305
           +  +S        +    GA  LLL PLC+P +   H+         L S  H     + 
Sbjct: 243 LLPSSGTYLASSPRWHFLGAIFLLLFPLCVPFLDYIHR--------ALESCFHHHSSGYA 302

Query: 306 LVNHELQQELIPIETERSGTKVITPFDYKEKESISWKMMGKENLTVLEEEHSAKMLVRRL 365
            VN E + +++ I++++S           E+ES         +   L +EHS  MLVR+L
Sbjct: 303 AVNIE-EPKILKIKSQKSNA---------EEES---------DQVRLGDEHSLGMLVRKL 362

Query: 366 EFWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTS-SLVTLYSSCSFFGRLISAGPDF 425
           EFWLY+VAYFCGGTIGLVYSNNLGQI+QSLG SS  + SLVTL+S+ SF GRL+S+ PDF
Sbjct: 363 EFWLYYVAYFCGGTIGLVYSNNLGQIAQSLGQSSSNAKSLVTLFSAFSFLGRLLSSAPDF 422

Query: 426 MREKV-HFARTGWLALALVPTPIAFILLAAS---GSEIALQIGTGLIGLSSGFMFSASVS 485
            R+K+ +  RTGW  ++L+PTP+AF +LA S        L++ T LIGLSSGF+F+A+VS
Sbjct: 423 TRKKLKYLTRTGWFTISLLPTPLAFFILAYSPKTNQTALLEVATALIGLSSGFVFAAAVS 482

Query: 486 ITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGR 545
           ITSELFG NS GVN NILITNIP+GS  YG +A   YD+ A    ++  A  D VVC+GR
Sbjct: 483 ITSELFGRNSVGVNQNILITNIPIGSLFYGYMAGSVYDTNASLGRKSVVA--DSVVCVGR 542

Query: 546 KCYLQTFIWWACISIIGLASCFLLFRRTKPAYDR 569
           KCY  TF++W C+S++G      LF RT+P Y R
Sbjct: 543 KCYFVTFLFWGCLSVLGFVCSLFLFIRTRPVYHR 545

[Close]

BLAST of Csor.00g235450 vs. TAIR 10
Match: AT2G30300.1 (Major facilitator superfamily protein )

HSP 1 Score: 293.9 bits (751), Expect = 2.8e-79
Identity = 203/546 (37.18%), Postives = 299/546 (54.76%), Query Frame = 0


Query: 29  WVALVAAIWIQAFTGTNFDFPSYSSNLKAALGMSQVELNYLAVASDVGKAFGWCSGIALL 88
           W++LVA +W+Q+F GTN  FP+YSS LK  L +SQ +LNYL+ ASD GK  G+ SGIA +
Sbjct: 8   WLSLVAILWLQSFNGTNLSFPAYSSQLKELLKISQFKLNYLSFASDAGKVLGFISGIAAV 67

Query: 89  YFPLWVVMLMAASMGFLGYGLQWLLLQGIVSLPYSMVYLLCLLAGCSICWFNTICYVSCI 148
           Y PL +V+L   S+GF GYGLQ+L          S++  +       ICW NT CY+  I
Sbjct: 68  YLPLPLVLLAGGSLGFAGYGLQYL----------SIIKKI-------ICWINTACYIVAI 127

Query: 149 QKFPTNRALALSLIVSFNGVSAALYTLMANAL----DPNDASLYLLLNALVPLIISVVTF 208
             FP NR +A+ +  S+ G+S  +YT M ++        +AS YLLLN+LVPL+  +VT 
Sbjct: 128 NSFPVNRQVAVGITASYQGLSGKIYTDMVHSFFHTSQREEASGYLLLNSLVPLVACLVT- 187

Query: 209 FPILHQPPAQLSSADATRNDSLTFICLYITAVITGLYLITFNSTPSSKYGAQLLLAGAFA 268
            P+L +      +   +++  + FI L++  + TG+Y +   +T      A L+L G   
Sbjct: 188 APMLMRHGGD-KTMSYSKDVKVGFIVLFVLTIATGIYAV---ATSLVSVPAVLVLVGIAL 247

Query: 269 LLLVPLCLPGIWSTHKWLFRIASTGLRSVLHFRFNLVNHELQQELIPIETERSGTKVITP 328
            LL PL +P               G + ++  R      + QQ++  +E        +  
Sbjct: 248 FLLAPLAIP------------IGVGFKELMSSR------KTQQKVHDLEAPVDKFYFVEE 307

Query: 329 FDYKEKESISWKMMGKENLTVLEEEHSAKMLVRRLEFWLYFVAYFCGGTIGLVYSNNLGQ 388
              KE+E     ++G      ++EE     L ++L+FW+YF  Y  G T+GLV++NNLGQ
Sbjct: 308 DHTKEEEEFEKAIIG------VKEEVEWTQLWKKLDFWIYFGLYLFGPTVGLVFTNNLGQ 367

Query: 389 ISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPD--FMREKVHFARTGWLALALVPTPIAF 448
           I++S G S+ TSSLV L SS  FFGRL+ +  D  F R K   +    +A +LV    +F
Sbjct: 368 IAESRG-STATSSLVALSSSFGFFGRLLPSLLDYFFSRNKYMPSSPVSMAGSLVAMVASF 427

Query: 449 ILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELFGPNSSGVNHNILITNIPLGSFL 508
           +LL    S+IAL IGT +IG+ SG + S SV++T+ELFG    GVNHNI++ +IPLGSF 
Sbjct: 428 LLLLID-SDIALYIGTAMIGIFSGALTSLSVTMTAELFGTKHFGVNHNIVVGSIPLGSFS 487

Query: 509 YGVLAAVSYDSTAGSSNQTAAALGDVVVCIGRKCYLQTFIWWACISIIGLASCFLLFRRT 568
           +G+LAA  Y          AA  GD   C G  C+  T ++W  +  I      +L+ R 
Sbjct: 488 FGLLAAKIY-------RDGAALYGDDGKCFGMHCFQTTLVFWGMLCSIAALLAAVLYIRN 498

The following BLAST results are available for this feature:

BLAST of Csor.00g235450 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I9E1	3.6e-156	55.05	Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ...	[more]
A0LNN5	1.3e-04	26.06	L-lactate transporter OS=Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) ...	[more]

BLAST of Csor.00g235450 vs. NCBI nr


Match Name	Identity	Description
KAG6593740.1	100.00	Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror...	[more]
KAG7026076.1	99.13	Protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022964108.1	98.96	protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 [Cucurbita moschata]	[more]
XP_022964107.1	97.77	protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 [Cucurbita moschata]	[more]
XP_023515123.1	97.05	protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Csor.00g235450 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1HJV4	98.96	protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1HGX4	97.77	protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1KEQ9	96.36	protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1KH74	95.21	protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 OS=Cucurbita maxima OX=3661 G...	[more]
A0A1S3CCZ1	83.33	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103499000 ...	[more]

BLAST of Csor.00g235450 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G45275.1	6.1e-175	58.01	Major facilitator superfamily protein	[more]
AT4G19450.1	2.6e-173	56.51	Major facilitator superfamily protein	[more]
AT1G31470.1	2.6e-157	55.05	Major facilitator superfamily protein	[more]
AT3G01630.1	5.1e-137	47.74	Major facilitator superfamily protein	[more]
AT2G30300.1	2.8e-79	37.18	Major facilitator superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 313..534 e-value: 4.7E-8 score: 34.2
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 4..207 e-value: 1.6E-5 score: 26.0
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 23..566
IPR010658	Nodulin-like	PFAM	PF06813	Nodulin-like	coord: 28..273 e-value: 1.9E-81 score: 273.2
None	No IPR available	PANTHER	PTHR21576	UNCHARACTERIZED NODULIN-LIKE PROTEIN	coord: 23..573
None	No IPR available	PANTHER	PTHR21576:SF134	NODULIN-LIKE/MFS TRANSPORTER	coord: 23..573

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g235450.m01	Csor.00g235450.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane