Csor.00g232180 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g232180
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	mitotic checkpoint serine/threonine-protein kinase BUB1
Location	Csor_Chr11: 14475231 .. 14479046 (+)
RNA-Seq Expression	Csor.00g232180
Synteny	Csor.00g232180

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGATAATCATCCCTGATTCTCAAATCACCACCACTACAACATCTGCTCAGGACCCTTTGTTACCTTGGCTCTGGTATTCCTTCATTTCTTTTCTGTAATCGAGCTGATTCACAATCGTTCTTCCATCCAGTTTCAGTGAAATTGTTGATGCACATTTGGTTCCAATCGCAGGTCCATAAAGGATGCTCTTGAAAATTCGACCTCCGGCAATGTTTCTGGGCCAGGTCTCGCGGATCTCGTTTCCGACTGTATTAAAACCTTCGGCGGCACTGTCAAGTACAAAAACGATGTCCGATTTCTCAAGATTTGGTTTCTCTCTGTGCGTAACTATCTTTCTTTCACTGTTATATCTGTGGGTTTAAAAGTTTAATCATCTTTGTTTCGTAGATGCAGTTGATTAGATAACTTTGACAAATTTTTAGTAATTTCGGTTTATTGATTTTGAATAATAATTAAGATTATATTGTTCGTGTAAGTAGATTGGTATGAGGGAGGATTTTGAAACCGGTTTCAAGGAATTATTGGAGCAGGAGATATGCATTGGCAATTCTTTGCTTTATATTTGGTATGCAGTGTTCCTTGAATCCAAGGGGAGATTAAACGACGCTAACATGATTTACGAGCTTGGCTTGTTGAGGTATCTCTATCTCCCTCTCATGGGCATACTGCTGAACACATGTATGATTATGCTTGTTCCTTACATTTTTTAACTGATAACAGGAACGCTGAGCCCCTTGAGTGGCTGAAAAAGGCAAAAGTGTTGTTTATTGATAGGATGTCTGAGTTAGTCAATACTCATTCAATGAAATTGGTGCGTGTTCTTGAAGCACAGAGCAAATGAGATTATGTTCCTTCAAGTATATAACGTAGGAATTTTGAATTCTATAAATTTCGTTTTTGTAGAATGATGGTGGTGAATCCACTAAATTTGTAGAATCTTACGTGAATCCATGGTCTTCCTCTGTTATGAAAGATTTATTAAGCAAGATTAATCTCAAGATTTTGAAGTATGAGGTAAGAAAGTGCAGTTTTCCTTGTCCAATGATGTATGCAAAGCTAGTTATTACTCTCTTACTAACTTGACTATGAATCTAATGCATGTTTCAGGGATACCATTCAAGCATTAAACCTTACCCTAAAAAGATGGCTCTAAATTCATTGCGGAATTCATCCAGGAATAAAGTTATTGAGATTGGTAATGGCATAGTTAGATTTTTCACCTAAATAGATAGAACTACCTTCAGTCAATCCTTCAAAACAGCGAGATTTCTTGAAATATTTTGTAAGAAATGGAGGGAAAAATGTTCTTATTTCATTCATACTCTATTCATCATGATAATTCTAATTCATTTGCCAAAATTTACAGGCGGGAAGAAATATGAGATCAAAGGTTGTGCAGGAACTGGCGGGTTTGCCCAAGTATATAAATCATATCTTGACAGTAACCCAGACGATGTTGTCGCACTAAAGGTAGAAAATCTGCATATACCCTTTACTTTAAGCATTCATAATACGCCATACCTGAATGTGAGCTTGCTGGATGTCCTGATTCCCTTCAAATGCTTGTCAGATACAAACACCTCCTTTTCCTTGGGAATTCTACATTTATCGTCAGCTTGATCAACGTGTCACCGTGAAGGAAGTATGTTGTAGCCCTTTAGATTTTCAGTAAGCGCTTATTACTTCTGAATGTTAGCAACTCGATTTATGCTCATCCTCAATTTCTCACAATGTCAGAGGTCAAGTTTTGGTTGTGCTCATGAGATGTATCTCTATTCTGACTATAGCATACTTATTTGTGACTACTTGGCTCATGGCACATTACAGGTGAATTTTCAGATTCTGAACCTTTCTGTACTGTATGGTCCTTTAAAATACTCACCGTAAGTAACTGTGTGGTTAAAACCTGGTGCAGGATGCCATTAATTTATATGTAGTCCTTGGGAAGACAATGGAAGAAGTTTTGTGTATTTATTACACCATAGAAATGCTTTACATGCTAGAGACTTTGCACAACATTGGCATTATCCATGGGGATTTCAAACCTGACAATCTGCTTATTCGTTATGCAAGGTAACAGTTGATTTCATTAAATAGTTCACTCTAGTTTTCATTTGCAACTTTGGCCCACAAATAATGATCCGCATCGGTGAGATCCCATGTCGGTTGGAAAGGAGAATATAACATTCTTTATAAGGGTGTGGAAACCTCTCCCTAGCAAATGCGTTTTAGAAACCTTGAGGAGAAGCCCGAAAGGAAAAGCCTAAAGAGGACAATATCTACTAACGGTGGACTTGGACTATTACAAACGGTATCAGAGCTGGACACCGAATGATATGCATGTGAGGAGGCTGAGTCTTGAAAGGGGGTGGACACGAGGCAGTGTGCCAACAAGGACGCTAGACCCTAAAGGGGCATGAATTGGAGGGCCCCACATAGATTGGAGAAAGGAACGAGTGCCAGCGAGGATGCCGGGCCCCGAAAGGGGTGGATTGTGAGATCCCACATTGGTTGAGGAGGAGAACAAAACATTCTTTATAAGGATGTGAAAACCTCTCCCTAGCAGGCGCGTTTTAAAAACCTTGAGGAAAGTCCAGGGAAGCCCAAAAAGGACAATATCTGCTATTAGTGCACTTAGAGCCGTTCCGGCATCGGACTGAAAAATTTTCTACTCCCATCCTATAATTCTCTACCAATTGACTTTAGTTTGGTTAACCATTATGCAGAGGCAAACTAACGGAAGATGGATTTCGGGAACGTAGTGGTCCTTGGAATGATCAGGTATGTAGGTTCATTATTCCTTGTTTTTGTTCCCAGGACATTACATTAGAGGAGCAAGGAAGATTACCTGTAGCTTTATCTTGTAGGGGCTTTGCCTTGTTGATTGGGGAAGAGGTATAGATCTGCAACTTTTTCCTGAAAATGTAGAATTTGAAGGAGATTGCCGCACTTCTGGCTTTCGTTGTACAGAAATGCTAGAGAATAAACCTTGGAAATATCAGGTCAGTCTCTTCTGCAGTGTGGTTATTTATATCAACTCTAGTGCTGCAAAACCATCGTGAAGAGGAAAACAAAAGTTGTATCAGTAGCTTTCTGTGTTTGTACAGGTTGACATATATGGCTTCTGTGTAGTCGTCCACACTATGCTTCATGGTTCCTACATGGACGTTGAGAAAAAAGTATCATCTGATGGGAGCTTTGTTTATTTACCCAAAACTTCTTTTAAGCGGTTAGTAACTTTTCTGGCGTCTTTACTCTGTTTCCTTTTCCTGTCCTGTGCAAAATACTTAGCCGTCACGCTTTTCTTTACATTATTAGTTGTTGCACATCAATTGCTACATCAATTATGTGAATAATGGAAATGATATTATGTATGCCTTTCATTTTTAAACATTAGCCGTCACTTCAGCGTCCATCTTAGTTTGACGAAGAAGCTCTTCAAAGATCTTTTTGGAACAAACTTTCATAAGGGTAGAAAAGAAACCTTTTGAAGTCTAAAGACATAGTTGAAACTTATCCGTAGATAGATTCTTTGTAGAGTCTGAGTTAGAGTTTGAGTGAGCAACCCACCATCCTCTTCCCTTCCTCTCTCTGACTTGAACTCTCTGTTGTTCCGGTATAGGTATTGGAACGTTGAACTGTGGAAGAATCTGTTCACGAAGTTGCTTAATATCAAACTAGGTGATTACAACATGGAGTTGTTGCAGAGTATTAGGGAATCATTCCAGGATTACATGTGCTCCAACCCTCAGCTTATAAGGAAGTTAAAGGAATTACTAGCAAAACAGAGGGCTTCCTTGTGTACAGCTTAG

mRNA sequence

ATGGCGATAATCATCCCTGATTCTCAAATCACCACCACTACAACATCTGCTCAGGACCCTTTGTTACCTTGGCTCTGGTCCATAAAGGATGCTCTTGAAAATTCGACCTCCGGCAATGTTTCTGGGCCAGGTCTCGCGGATCTCGTTTCCGACTGTATTAAAACCTTCGGCGGCACTGTCAAGTACAAAAACGATGTCCGATTTCTCAAGATTTGGTTTCTCTCTATTGGTATGAGGGAGGATTTTGAAACCGGTTTCAAGGAATTATTGGAGCAGGAGATATGCATTGGCAATTCTTTGCTTTATATTTGGTATGCAGTGTTCCTTGAATCCAAGGGGAGATTAAACGACGCTAACATGATTTACGAGCTTGGCTTGTTGAGGAACGCTGAGCCCCTTGAGTGGCTGAAAAAGGCAAAAGTGTTGTTTATTGATAGGATGTCTGAGTTAGTCAATACTCATTCAATGAAATTGAATGATGGTGGTGAATCCACTAAATTTGTAGAATCTTACGTGAATCCATGGTCTTCCTCTGTTATGAAAGATTTATTAAGCAAGATTAATCTCAAGATTTTGAAGTATGAGGGATACCATTCAAGCATTAAACCTTACCCTAAAAAGATGGCTCTAAATTCATTGCGGAATTCATCCAGGAATAAAGTTATTGAGATTGGCGGGAAGAAATATGAGATCAAAGGTTGTGCAGGAACTGGCGGGTTTGCCCAAGTATATAAATCATATCTTGACAGTAACCCAGACGATGTTGTCGCACTAAAGATACAAACACCTCCTTTTCCTTGGGAATTCTACATTTATCGTCAGCTTGATCAACGTGTCACCGTGAAGGAAAGGTCAAGTTTTGGTTGTGCTCATGAGATGTATCTCTATTCTGACTATAGCATACTTATTTGTGACTACTTGGCTCATGGCACATTACAGGATGCCATTAATTTATATGTAGTCCTTGGGAAGACAATGGAAGAAGTTTTGTGTATTTATTACACCATAGAAATGCTTTACATGCTAGAGACTTTGCACAACATTGGCATTATCCATGGGGATTTCAAACCTGACAATCTGCTTATTCGTTATGCAAGAGGCAAACTAACGGAAGATGGATTTCGGGAACGTAGTGGTCCTTGGAATGATCAGGGGCTTTGCCTTGTTGATTGGGGAAGAGGTATAGATCTGCAACTTTTTCCTGAAAATGTAGAATTTGAAGGAGATTGCCGCACTTCTGGCTTTCGTTGTACAGAAATGCTAGAGAATAAACCTTGGAAATATCAGGTTGACATATATGGCTTCTGTGTAGTCGTCCACACTATGCTTCATGGTTCCTACATGGACGTTGAGAAAAAAGTATCATCTGATGGGAGCTTTGTTTATTTACCCAAAACTTCTTTTAAGCGGTATTGGAACGTTGAACTGTGGAAGAATCTGTTCACGAAGTTGCTTAATATCAAACTAGGTGATTACAACATGGAGTTGTTGCAGAGTATTAGGGAATCATTCCAGGATTACATGTGCTCCAACCCTCAGCTTATAAGGAAGTTAAAGGAATTACTAGCAAAACAGAGGGCTTCCTTGTGTACAGCTTAG

Coding sequence (CDS)

ATGGCGATAATCATCCCTGATTCTCAAATCACCACCACTACAACATCTGCTCAGGACCCTTTGTTACCTTGGCTCTGGTCCATAAAGGATGCTCTTGAAAATTCGACCTCCGGCAATGTTTCTGGGCCAGGTCTCGCGGATCTCGTTTCCGACTGTATTAAAACCTTCGGCGGCACTGTCAAGTACAAAAACGATGTCCGATTTCTCAAGATTTGGTTTCTCTCTATTGGTATGAGGGAGGATTTTGAAACCGGTTTCAAGGAATTATTGGAGCAGGAGATATGCATTGGCAATTCTTTGCTTTATATTTGGTATGCAGTGTTCCTTGAATCCAAGGGGAGATTAAACGACGCTAACATGATTTACGAGCTTGGCTTGTTGAGGAACGCTGAGCCCCTTGAGTGGCTGAAAAAGGCAAAAGTGTTGTTTATTGATAGGATGTCTGAGTTAGTCAATACTCATTCAATGAAATTGAATGATGGTGGTGAATCCACTAAATTTGTAGAATCTTACGTGAATCCATGGTCTTCCTCTGTTATGAAAGATTTATTAAGCAAGATTAATCTCAAGATTTTGAAGTATGAGGGATACCATTCAAGCATTAAACCTTACCCTAAAAAGATGGCTCTAAATTCATTGCGGAATTCATCCAGGAATAAAGTTATTGAGATTGGCGGGAAGAAATATGAGATCAAAGGTTGTGCAGGAACTGGCGGGTTTGCCCAAGTATATAAATCATATCTTGACAGTAACCCAGACGATGTTGTCGCACTAAAGATACAAACACCTCCTTTTCCTTGGGAATTCTACATTTATCGTCAGCTTGATCAACGTGTCACCGTGAAGGAAAGGTCAAGTTTTGGTTGTGCTCATGAGATGTATCTCTATTCTGACTATAGCATACTTATTTGTGACTACTTGGCTCATGGCACATTACAGGATGCCATTAATTTATATGTAGTCCTTGGGAAGACAATGGAAGAAGTTTTGTGTATTTATTACACCATAGAAATGCTTTACATGCTAGAGACTTTGCACAACATTGGCATTATCCATGGGGATTTCAAACCTGACAATCTGCTTATTCGTTATGCAAGAGGCAAACTAACGGAAGATGGATTTCGGGAACGTAGTGGTCCTTGGAATGATCAGGGGCTTTGCCTTGTTGATTGGGGAAGAGGTATAGATCTGCAACTTTTTCCTGAAAATGTAGAATTTGAAGGAGATTGCCGCACTTCTGGCTTTCGTTGTACAGAAATGCTAGAGAATAAACCTTGGAAATATCAGGTTGACATATATGGCTTCTGTGTAGTCGTCCACACTATGCTTCATGGTTCCTACATGGACGTTGAGAAAAAAGTATCATCTGATGGGAGCTTTGTTTATTTACCCAAAACTTCTTTTAAGCGGTATTGGAACGTTGAACTGTGGAAGAATCTGTTCACGAAGTTGCTTAATATCAAACTAGGTGATTACAACATGGAGTTGTTGCAGAGTATTAGGGAATCATTCCAGGATTACATGTGCTCCAACCCTCAGCTTATAAGGAAGTTAAAGGAATTACTAGCAAAACAGAGGGCTTCCTTGTGTACAGCTTAG

Protein sequence

MAIIIPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTVKYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANMIYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVMKDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYSILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNLLIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEMLENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNLFTKLLNIKLGDYNMELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA

Homology

[Close]

BLAST of Csor.00g232180 vs. ExPASy Swiss-Prot
Match: F4IVI0 (Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Arabidopsis thaliana OX=3702 GN=BUB1 PE=1 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 6.5e-192
Identity = 309/518 (59.65%), Postives = 402/518 (77.61%), Query Frame = 0


Query: 16  SAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTVKYKNDVRFLKIWFLS 75
           +A DPL PWL  IK+++E+  +G  SG  L  L+ DCI T+    +Y+ND+RFLKIWFL 
Sbjct: 8   AAGDPLFPWLMEIKNSMEDLYAGKNSGYDLDKLLFDCISTYKKDSRYRNDLRFLKIWFLY 67

Query: 76  IGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANMIYELGLLRNAEPLEW 135
           +  REDFE  ++E+ E EIC G+SLLY WYA+FLE KG    AN +Y+ GL R AEP + 
Sbjct: 68  LEGREDFERVYREIEETEICKGHSLLYEWYAIFLEVKGLWRRANSVYQTGLSRKAEPFDR 127

Query: 136 LKKAKVLFIDRMSELVNTHSM-KLNDGGESTKFVESYVNPWSSSVMKDLLSKINLKILKY 195
           LK+A  LF+ R+S+     S+ K+ D  ++T     +VNPW +S +  L+ KI  +++KY
Sbjct: 128 LKEAHSLFLQRISKRTKASSLEKVGDDAQATDLETGFVNPWETSTVNGLIHKIKPQLVKY 187

Query: 196 EGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDD 255
           +GYH S K +P K  L+SL+N SRNK+IEIGG+KY++KGCAG GGFAQV+K+++DSNPD+
Sbjct: 188 DGYHVSNKVFPGKANLSSLQNYSRNKIIEIGGRKYQMKGCAGQGGFAQVFKAFIDSNPDE 247

Query: 256 VVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYSILICDYLAHGTLQD 315
           VVALK+Q PPFPWEF++YRQLD R+   +RSSFG A  +++YSDYSIL+CDYL+HGTLQD
Sbjct: 248 VVALKVQKPPFPWEFHMYRQLDCRIPDSQRSSFGLAQRVHVYSDYSILVCDYLSHGTLQD 307

Query: 316 AINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNLLIRYARGKLTEDGF 375
            IN YVV+GK+MEEVLC+YYTIEML MLETLH++GIIHGDFKPDNLLIRY    LTE GF
Sbjct: 308 VINSYVVVGKSMEEVLCMYYTIEMLNMLETLHSVGIIHGDFKPDNLLIRYPPENLTETGF 367

Query: 376 RERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEMLENKPWKYQVDIYG 435
            E++G W+ +GLCLVDWGRGIDL LFP   EF GDCRTSGFRC EM E+KPWK+QVD YG
Sbjct: 368 HEKTGSWSKKGLCLVDWGRGIDLSLFPRTTEFTGDCRTSGFRCVEMKEDKPWKFQVDTYG 427

Query: 436 FCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNLFTKLLNIKLGDYNM 495
            CV+VH MLH  YM++EKK S DG ++ +P+TSFKRYW V+LWK LFTKLLN +  + + 
Sbjct: 428 LCVIVHMMLHNVYMEIEKKQSLDGGYINMPRTSFKRYWKVDLWKELFTKLLNRETCEDDT 487

Query: 496 ELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 533
           E L+++R+S ++Y+CS+P+L++KL ELLAKQR SLC++
Sbjct: 488 ETLRNLRKSMEEYICSDPKLMKKLNELLAKQRISLCSS 525

[Close]

BLAST of Csor.00g232180 vs. ExPASy Swiss-Prot
Match: O43683 (Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Homo sapiens OX=9606 GN=BUB1 PE=1 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 1.2e-47
Identity = 119/353 (33.71%), Postives = 184/353 (52.12%), Query Frame = 0


Query: 173  NPWSSSVMKDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKV-IEIGGKKYEI 232
            NPW   ++  LLS          G    +  YP          + + K   ++G K   +
Sbjct: 740  NPWDDKLIFKLLS----------GLSKPVSSYPNTFEWQCKLPAIKPKTEFQLGSKLVYV 799

Query: 233  KGCAGTGGFAQVYKS----YLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSF 292
                G G FAQVY++      D+       LK+Q P  PWEFYI  QL +R+    +  F
Sbjct: 800  HHLLGEGAFAQVYEATQGDLNDAKNKQKFVLKVQKPANPWEFYIGTQLMERLKPSMQHMF 859

Query: 293  GCAHEMYLYSDYSILICDYLAHGTLQDAINLYV-VLGKTMEEVLCIYYTIEMLYMLETLH 352
               +  +L+ + S+L+ +  ++GTL +AINLY     K M + L I + + MLYM+E +H
Sbjct: 860  MKFYSAHLFQNGSVLVGELYSYGTLLNAINLYKNTPEKVMPQGLVISFAMRMLYMIEQVH 919

Query: 353  NIGIIHGDFKPDNLLIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEF 412
            +  IIHGD KPDN ++    G L +D   + S      GL L+D G+ ID++LFP+   F
Sbjct: 920  DCEIIHGDIKPDNFIL--GNGFLEQDDEDDLSA-----GLALIDLGQSIDMKLFPKGTIF 979

Query: 413  EGDCRTSGFRCTEMLENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKT 472
               C TSGF+C EML NKPW YQ+D +G    V+ ML G+YM    KV ++G     P+ 
Sbjct: 980  TAKCETSGFQCVEMLSNKPWNYQIDYFGVAATVYCMLFGTYM----KVKNEGG-ECKPEG 1039

Query: 473  SFKRYWNVELWKNLFTKLLNIKLGDY--NMELL-QSIRESFQDYMCSNPQLIR 517
             F+R  ++++W   F  +LNI    +  +++LL Q +++ FQ +  +  + +R
Sbjct: 1040 LFRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKVFQQHYTNKIRALR 1070

[Close]

BLAST of Csor.00g232180 vs. ExPASy Swiss-Prot
Match: O08901 (Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Mus musculus OX=10090 GN=Bub1 PE=1 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 3.3e-47
Identity = 114/354 (32.20%), Postives = 187/354 (52.82%), Query Frame = 0


Query: 173  NPWSSSVMKDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIK 232
            NPW   ++  LLS ++  +  Y         +  +  L +++  +     ++G     + 
Sbjct: 714  NPWDDELILKLLSGLSKPVTSYS------NTFEWQSKLPAIKTKTE---YQLGSLLVYVN 773

Query: 233  GCAGTGGFAQVYKS----YLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFG 292
               G G FAQV+++      ++  +    LK+Q P   WEFYI  QL +R+  +    F 
Sbjct: 774  HLLGEGAFAQVFEAIHGDVRNAKSEQKCILKVQRPANSWEFYIGMQLMERLKPEVHHMFI 833

Query: 293  CAHEMYLYSDYSILICDYLAHGTLQDAINLYV-VLGKTMEEVLCIYYTIEMLYMLETLHN 352
              +  +L+ + SIL+ +  ++GTL + INLY     K M + L + + I MLYM+E +H+
Sbjct: 834  KFYSAHLFKNGSILVGELYSYGTLLNVINLYKNTSEKVMPQALVLTFAIRMLYMVEQVHS 893

Query: 353  IGIIHGDFKPDNLLIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFE 412
              IIHGD KPDN ++ +         F E++      GL L+D G+ ID++LFP+   F 
Sbjct: 894  CEIIHGDIKPDNFILGHR--------FLEQADEDLATGLALIDLGQSIDMKLFPKGTVFT 953

Query: 413  GDCRTSGFRCTEMLENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTS 472
            G C TSGF+C EML NKPW YQ+D +G    ++ ML GSYM    KV ++G  V+ P+  
Sbjct: 954  GKCETSGFQCPEMLSNKPWNYQIDYFGVAATIYCMLFGSYM----KVKNEGG-VWKPEGL 1013

Query: 473  FKRYWNVELWKNLFTKLLNIKLGDYNMELLQSIRESFQDYMCSNPQLIRKLKEL 522
            F+R  ++++W+  F  +LNI    +N+  L  +R++ +  +    Q   K+K L
Sbjct: 1014 FRRLPHLDMWEEFFHIMLNIP-DCHNLPSLDFLRQNMKKLL--EQQYSNKIKTL 1042

HSP 2 Score: 53.5 bits (127), Expect = 8.3e-06
Identity = 40/133 (30.08%), Postives = 59/133 (44.36%), Query Frame = 0


Query: 16  SAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTVKYKNDVRFLKIWFLS 75
           +  DPL  W   IK   EN          L  L+   +K F     Y ND RF+      
Sbjct: 19  TGNDPLGEWESFIKWVEENFPDNK---EYLMTLLEHLMKEFLHKKNYHNDSRFINYCLKF 78

Query: 76  IGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANMIYELGLLRNAEPLEW 135
                D    F+ L  Q I   +S +Y+ +A  LE++G L  A+ I++ G+   AEP E 
Sbjct: 79  AEYNSDRHQFFEFLYNQGIGTKSSYIYMSWAGHLEAQGELQHASAIFQTGIHNEAEPKEL 138

Query: 136 LKKAKVLFIDRMS 149
           L++   LF  R++
Sbjct: 139 LQQQYRLFQARLT 148

[Close]

BLAST of Csor.00g232180 vs. ExPASy Swiss-Prot
Match: P41695 (Checkpoint serine/threonine-protein kinase BUB1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=BUB1 PE=1 SV=2)

HSP 1 Score: 187.6 bits (475), Expect = 3.7e-46
Identity = 107/331 (32.33%), Postives = 178/331 (53.78%), Query Frame = 0


Query: 173 NPWSSSVMKDLLSKINLKILKYEGYHSSIKPYPKKMALNSL--------RNSSRNKVIEI 232
           NP S+++    LS+I+  + +Y  +++    Y +++ ++SL        RN ++N +++ 
Sbjct: 643 NPLSNNLRAKFLSEISPPLFQYNTFYN----YNQELKMSSLLKKIHRVSRNENKNPIVDF 702

Query: 233 --GGKKYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVK 292
              G  Y I+G  G GG+A VY +  +S+   + ALK++ P   WE+YI  Q++ R+   
Sbjct: 703 KKTGDLYCIRGELGEGGYATVYLA--ESSQGHLRALKVEKPASVWEYYIMSQVEFRLRKS 762

Query: 293 E-RSSFGCAHEMYLYSDYSILICDYLAHGTLQDAINLY----VVLGKTMEEVLCIYYTIE 352
               S   A  ++L+ D S L+ +Y + GT+ D INL     +     M+E LC++ T+E
Sbjct: 763 TILKSIINASALHLFLDESYLVLNYASQGTVLDLINLQREKAIDGNGIMDEYLCMFITVE 822

Query: 353 MLYMLETLHNIGIIHGDFKPDNLLIRYAR--GKLTEDGFRERSGPWNDQGLCLVDWGRGI 412
           ++ +LE +H +GIIHGD KPDN +IR  +    L     R     W ++G+ L+D+GR  
Sbjct: 823 LMKVLEKIHEVGIIHGDLKPDNCMIRLEKPGEPLGAHYMRNGEDGWENKGIYLIDFGRSF 882

Query: 413 DLQLFPENVEFEGDCRTSGFRCTEMLENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVS 472
           D+ L P   +F+ + +     C EM   KPW Y+ D YG   V+H+ML G +++  +  +
Sbjct: 883 DMTLLPPGTKFKSNWKADQQDCWEMRAGKPWSYEADYYGLAGVIHSMLFGKFIETIQLQN 942

Query: 473 SDGSFVYLPKTSFKRYWNVELWKNLFTKLLN 487
                    K  FKRYW  E+W  +F  LLN
Sbjct: 943 GRCKL----KNPFKRYWKKEIWGVIFDLLLN 963

[Close]

BLAST of Csor.00g232180 vs. ExPASy Swiss-Prot
Match: O94751 (Checkpoint serine/threonine-protein kinase bub1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=bub1 PE=1 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 3.2e-42
Identity = 110/313 (35.14%), Postives = 168/313 (53.67%), Query Frame = 0


Query: 236  GTGGFAQVY--KSYLDSNPDDV------------VALKIQTPPFPWEFYIYRQLDQRVT- 295
            G G FA VY  KS +++   DV             ALKI+TPP  +EFY+ RQ   R+  
Sbjct: 725  GQGAFAPVYLVKSKIETENGDVSQGGAENNESKLFALKIETPPSCFEFYLTRQAMTRLKG 784

Query: 296  VKERSSFGCAHEMYLYSDYSILICDYLAHGTLQDAINLY---VVLGKTMEEVLCIYYTIE 355
            ++E +S    H+++++ D S L+ DY   G++ D +N           M+E+L ++++IE
Sbjct: 785  LRETNSILPVHQLHMFHDTSHLLMDYRPQGSILDLVNSMHNSTFSSSGMDEILVVFFSIE 844

Query: 356  MLYMLETLHNIGIIHGDFKPDNLLIR---YARGKLTEDGFRERSGPWNDQGLCLVDWGRG 415
             L ++E LH   IIHGD K DN L+R    A  + +     E    W+ +G+ L+D+GRG
Sbjct: 845  FLRIIEALHTHKIIHGDLKADNALLRLETVADSEWSPIYSPEGLYGWSFKGIYLIDFGRG 904

Query: 416  IDLQLFPENVEFEGDCRTSGFRCTEMLENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKV 475
            IDL LF E V+F  D  T    C EM E +PW YQ+D +G   +++TML G Y++   +V
Sbjct: 905  IDLSLFEEKVKFIADWDTDLQDCIEMREGRPWTYQIDYHGLAAIIYTMLFGQYIETRIEV 964

Query: 476  SSDGSFVYLPKTSFKRYWNVELWKNLFTKLLN--IKLGDYNM---ELLQSIRESFQDYMC 523
              +G    +     KRYWN +LW  LF  LLN  + + + N+   E L  IR   ++++ 
Sbjct: 965  -INGQRRQVLTQRMKRYWNQDLWHRLFDLLLNPTLHVSEENLPMTEELSKIRIEMEEWLV 1024

[Close]

BLAST of Csor.00g232180 vs. NCBI nr
Match: KAG6589257.1 (Mitotic checkpoint serine/threonine-protein kinase BUB1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1094 bits (2830), Expect = 0.0
Identity = 532/532 (100.00%), Postives = 532/532 (100.00%), Query Frame = 0


Query: 1   MAIIIPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV 60
           MAIIIPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV
Sbjct: 1   MAIIIPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120

Query: 121 IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM 180
           IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM
Sbjct: 121 IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM 180

Query: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240
           KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF
Sbjct: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL
Sbjct: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360

Query: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420
           LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM
Sbjct: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480
           LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL
Sbjct: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480

Query: 481 FTKLLNIKLGDYNMELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 532
           FTKLLNIKLGDYNMELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA
Sbjct: 481 FTKLLNIKLGDYNMELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 532

[Close]

BLAST of Csor.00g232180 vs. NCBI nr
Match: XP_022930700.1 (mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucurbita moschata])

HSP 1 Score: 1092 bits (2824), Expect = 0.0
Identity = 531/532 (99.81%), Postives = 531/532 (99.81%), Query Frame = 0


Query: 1   MAIIIPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV 60
           MAIIIPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV
Sbjct: 58  MAIIIPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV 117

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM
Sbjct: 118 KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 177

Query: 121 IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM 180
           IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM
Sbjct: 178 IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM 237

Query: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240
           KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF
Sbjct: 238 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 297

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS
Sbjct: 298 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 357

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL
Sbjct: 358 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 417

Query: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420
           LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM
Sbjct: 418 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 477

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480
           LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL
Sbjct: 478 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 537

Query: 481 FTKLLNIKLGDYNMELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 532
           FTKLLNIKLGDYN ELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA
Sbjct: 538 FTKLLNIKLGDYNKELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 589

[Close]

BLAST of Csor.00g232180 vs. NCBI nr
Match: KAG7022957.1 (Mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1086 bits (2808), Expect = 0.0
Identity = 530/534 (99.25%), Postives = 531/534 (99.44%), Query Frame = 0


Query: 1   MAIIIPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV 60
           MAIIIPDSQITTTTTS+QDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV
Sbjct: 1   MAIIIPDSQITTTTTSSQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120

Query: 121 IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM 180
           IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM
Sbjct: 121 IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM 180

Query: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIG--GKKYEIKGCAGTG 240
           KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIG  GKKYEIKGCAGTG
Sbjct: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGNGGKKYEIKGCAGTG 240

Query: 241 GFAQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSD 300
           GFAQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSD
Sbjct: 241 GFAQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSD 300

Query: 301 YSILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPD 360
           YSILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPD
Sbjct: 301 YSILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPD 360

Query: 361 NLLIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCT 420
           NLLIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCT
Sbjct: 361 NLLIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCT 420

Query: 421 EMLENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWK 480
           EMLENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWK
Sbjct: 421 EMLENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWK 480

Query: 481 NLFTKLLNIKLGDYNMELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 532
           NLFTKLLNIKLGDYN ELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA
Sbjct: 481 NLFTKLLNIKLGDYNKELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 534

[Close]

BLAST of Csor.00g232180 vs. NCBI nr
Match: XP_022989217.1 (mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucurbita maxima])

HSP 1 Score: 1079 bits (2791), Expect = 0.0
Identity = 523/532 (98.31%), Postives = 527/532 (99.06%), Query Frame = 0


Query: 1   MAIIIPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV 60
           MAII PDSQIT+TTTS QDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFG TV
Sbjct: 1   MAIIFPDSQITSTTTSVQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGSTV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120

Query: 121 IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM 180
           IYE+GLLRNAEPLEWLKKAK+LFIDRMSELVNTHSMKLNDGGESTKFVE+YVNPWSSSVM
Sbjct: 121 IYEIGLLRNAEPLEWLKKAKMLFIDRMSELVNTHSMKLNDGGESTKFVETYVNPWSSSVM 180

Query: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240
           KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF
Sbjct: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL
Sbjct: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360

Query: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420
           LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM
Sbjct: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480
           LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL
Sbjct: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480

Query: 481 FTKLLNIKLGDYNMELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 532
           FTKLLNIKLGDYN ELLQSIR SFQDYMCSNPQLIRKLKELLAKQRASLCTA
Sbjct: 481 FTKLLNIKLGDYNKELLQSIRGSFQDYMCSNPQLIRKLKELLAKQRASLCTA 532

[Close]

BLAST of Csor.00g232180 vs. NCBI nr
Match: XP_023531077.1 (mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1077 bits (2784), Expect = 0.0
Identity = 523/532 (98.31%), Postives = 525/532 (98.68%), Query Frame = 0


Query: 1   MAIIIPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV 60
           MAII PDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGG V
Sbjct: 1   MAIIFPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGNV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120

Query: 121 IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM 180
           IYELGLLRNAEPLEWLKKAK+LFIDRMSELVNT SMKLNDGGESTK VE+YVNPWSSSVM
Sbjct: 121 IYELGLLRNAEPLEWLKKAKMLFIDRMSELVNTRSMKLNDGGESTKIVETYVNPWSSSVM 180

Query: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240
           KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF
Sbjct: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLD RVTVKERSSFGCAHEMYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDHRVTVKERSSFGCAHEMYLYSDYS 300

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNI IIHGDFKPDNL
Sbjct: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNISIIHGDFKPDNL 360

Query: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420
           LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM
Sbjct: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480
           LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL
Sbjct: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480

Query: 481 FTKLLNIKLGDYNMELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 532
           FTKLLNIKLGDYN ELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA
Sbjct: 481 FTKLLNIKLGDYNKELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 532

[Close]

BLAST of Csor.00g232180 vs. ExPASy TrEMBL
Match: A0A6J1EXJ7 (mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Cucurbita moschata OX=3662 GN=LOC111437097 PE=4 SV=1)

HSP 1 Score: 1092 bits (2824), Expect = 0.0
Identity = 531/532 (99.81%), Postives = 531/532 (99.81%), Query Frame = 0


Query: 1   MAIIIPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV 60
           MAIIIPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV
Sbjct: 58  MAIIIPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV 117

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM
Sbjct: 118 KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 177

Query: 121 IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM 180
           IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM
Sbjct: 178 IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM 237

Query: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240
           KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF
Sbjct: 238 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 297

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS
Sbjct: 298 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 357

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL
Sbjct: 358 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 417

Query: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420
           LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM
Sbjct: 418 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 477

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480
           LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL
Sbjct: 478 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 537

Query: 481 FTKLLNIKLGDYNMELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 532
           FTKLLNIKLGDYN ELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA
Sbjct: 538 FTKLLNIKLGDYNKELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 589

[Close]

BLAST of Csor.00g232180 vs. ExPASy TrEMBL
Match: A0A6J1JF74 (mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Cucurbita maxima OX=3661 GN=LOC111486358 PE=4 SV=1)

HSP 1 Score: 1079 bits (2791), Expect = 0.0
Identity = 523/532 (98.31%), Postives = 527/532 (99.06%), Query Frame = 0


Query: 1   MAIIIPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV 60
           MAII PDSQIT+TTTS QDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFG TV
Sbjct: 1   MAIIFPDSQITSTTTSVQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGSTV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120

Query: 121 IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM 180
           IYE+GLLRNAEPLEWLKKAK+LFIDRMSELVNTHSMKLNDGGESTKFVE+YVNPWSSSVM
Sbjct: 121 IYEIGLLRNAEPLEWLKKAKMLFIDRMSELVNTHSMKLNDGGESTKFVETYVNPWSSSVM 180

Query: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240
           KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF
Sbjct: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL
Sbjct: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360

Query: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420
           LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM
Sbjct: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480
           LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL
Sbjct: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480

Query: 481 FTKLLNIKLGDYNMELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 532
           FTKLLNIKLGDYN ELLQSIR SFQDYMCSNPQLIRKLKELLAKQRASLCTA
Sbjct: 481 FTKLLNIKLGDYNKELLQSIRGSFQDYMCSNPQLIRKLKELLAKQRASLCTA 532

[Close]

BLAST of Csor.00g232180 vs. ExPASy TrEMBL
Match: A0A6J1D2B6 (mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111016707 PE=4 SV=1)

HSP 1 Score: 975 bits (2521), Expect = 0.0
Identity = 466/532 (87.59%), Postives = 491/532 (92.29%), Query Frame = 0


Query: 1   MAIIIPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV 60
           MAII PDSQ  T+T SAQDPLLPWLWSIKDALENSTSGN SGP LA L+SDCIK FGG V
Sbjct: 1   MAIIFPDSQ-NTSTISAQDPLLPWLWSIKDALENSTSGNASGPDLAKLLSDCIKNFGGNV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120
           KYKND+RFLKIWFLSIG+REDFETGFKELLEQEICIGNSLLYIWYA FLESKG+L+DANM
Sbjct: 61  KYKNDIRFLKIWFLSIGLREDFETGFKELLEQEICIGNSLLYIWYAAFLESKGKLHDANM 120

Query: 121 IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM 180
           +YELGLLRNAEP EWLKKAK LFIDRMSELV+ HS ++NDGGES KF E+YVNPWS S+M
Sbjct: 121 VYELGLLRNAEPFEWLKKAKTLFIDRMSELVSIHSTQVNDGGESNKFAETYVNPWSPSIM 180

Query: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240
           KDLLSKIN KILKYEGYHSS K Y +KMALNSLRNSSRNK IEIGGK+YEIKGCAGTGGF
Sbjct: 181 KDLLSKINPKILKYEGYHSSSKAYSRKMALNSLRNSSRNKTIEIGGKRYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300
           AQVYK+Y+D NP+DVVALKIQTPPFPWEFY+YRQLDQ VT+KERSSFGCAH MY YSDYS
Sbjct: 241 AQVYKAYVDGNPEDVVALKIQTPPFPWEFYMYRQLDQHVTMKERSSFGCAHAMYHYSDYS 300

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLH  GIIHGDFKPDNL
Sbjct: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHGFGIIHGDFKPDNL 360

Query: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420
           LIRYAR  LTEDGFRERSGPW DQGLCLVDWGRGIDL LFPENVEF+GDCRTSGFRCTEM
Sbjct: 361 LIRYARDNLTEDGFRERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCTEM 420

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480
           LENKPWKYQVDIYG CVVVHTMLHGSYMDV+KK SS+G F+YLPKTSFKRYWN+ELWKNL
Sbjct: 421 LENKPWKYQVDIYGLCVVVHTMLHGSYMDVDKKASSNGGFIYLPKTSFKRYWNIELWKNL 480

Query: 481 FTKLLNIKLGDYNMELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 532
           FTKLLNI  GDYN +LLQSIR SFQDYMCSNP LIRKLKELL KQRASLCTA
Sbjct: 481 FTKLLNITPGDYNQKLLQSIRVSFQDYMCSNPHLIRKLKELLVKQRASLCTA 531

[Close]

BLAST of Csor.00g232180 vs. ExPASy TrEMBL
Match: A0A6J1D1C8 (mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016707 PE=4 SV=1)

HSP 1 Score: 975 bits (2521), Expect = 0.0
Identity = 466/532 (87.59%), Postives = 491/532 (92.29%), Query Frame = 0


Query: 1   MAIIIPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV 60
           MAII PDSQ  T+T SAQDPLLPWLWSIKDALENSTSGN SGP LA L+SDCIK FGG V
Sbjct: 143 MAIIFPDSQ-NTSTISAQDPLLPWLWSIKDALENSTSGNASGPDLAKLLSDCIKNFGGNV 202

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120
           KYKND+RFLKIWFLSIG+REDFETGFKELLEQEICIGNSLLYIWYA FLESKG+L+DANM
Sbjct: 203 KYKNDIRFLKIWFLSIGLREDFETGFKELLEQEICIGNSLLYIWYAAFLESKGKLHDANM 262

Query: 121 IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM 180
           +YELGLLRNAEP EWLKKAK LFIDRMSELV+ HS ++NDGGES KF E+YVNPWS S+M
Sbjct: 263 VYELGLLRNAEPFEWLKKAKTLFIDRMSELVSIHSTQVNDGGESNKFAETYVNPWSPSIM 322

Query: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240
           KDLLSKIN KILKYEGYHSS K Y +KMALNSLRNSSRNK IEIGGK+YEIKGCAGTGGF
Sbjct: 323 KDLLSKINPKILKYEGYHSSSKAYSRKMALNSLRNSSRNKTIEIGGKRYEIKGCAGTGGF 382

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300
           AQVYK+Y+D NP+DVVALKIQTPPFPWEFY+YRQLDQ VT+KERSSFGCAH MY YSDYS
Sbjct: 383 AQVYKAYVDGNPEDVVALKIQTPPFPWEFYMYRQLDQHVTMKERSSFGCAHAMYHYSDYS 442

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLH  GIIHGDFKPDNL
Sbjct: 443 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHGFGIIHGDFKPDNL 502

Query: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420
           LIRYAR  LTEDGFRERSGPW DQGLCLVDWGRGIDL LFPENVEF+GDCRTSGFRCTEM
Sbjct: 503 LIRYARDNLTEDGFRERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCTEM 562

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480
           LENKPWKYQVDIYG CVVVHTMLHGSYMDV+KK SS+G F+YLPKTSFKRYWN+ELWKNL
Sbjct: 563 LENKPWKYQVDIYGLCVVVHTMLHGSYMDVDKKASSNGGFIYLPKTSFKRYWNIELWKNL 622

Query: 481 FTKLLNIKLGDYNMELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 532
           FTKLLNI  GDYN +LLQSIR SFQDYMCSNP LIRKLKELL KQRASLCTA
Sbjct: 623 FTKLLNITPGDYNQKLLQSIRVSFQDYMCSNPHLIRKLKELLVKQRASLCTA 673

[Close]

BLAST of Csor.00g232180 vs. ExPASy TrEMBL
Match: A0A6J1D2I5 (mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016707 PE=4 SV=1)

HSP 1 Score: 975 bits (2521), Expect = 0.0
Identity = 466/532 (87.59%), Postives = 491/532 (92.29%), Query Frame = 0


Query: 1   MAIIIPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV 60
           MAII PDSQ  T+T SAQDPLLPWLWSIKDALENSTSGN SGP LA L+SDCIK FGG V
Sbjct: 131 MAIIFPDSQ-NTSTISAQDPLLPWLWSIKDALENSTSGNASGPDLAKLLSDCIKNFGGNV 190

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120
           KYKND+RFLKIWFLSIG+REDFETGFKELLEQEICIGNSLLYIWYA FLESKG+L+DANM
Sbjct: 191 KYKNDIRFLKIWFLSIGLREDFETGFKELLEQEICIGNSLLYIWYAAFLESKGKLHDANM 250

Query: 121 IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM 180
           +YELGLLRNAEP EWLKKAK LFIDRMSELV+ HS ++NDGGES KF E+YVNPWS S+M
Sbjct: 251 VYELGLLRNAEPFEWLKKAKTLFIDRMSELVSIHSTQVNDGGESNKFAETYVNPWSPSIM 310

Query: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240
           KDLLSKIN KILKYEGYHSS K Y +KMALNSLRNSSRNK IEIGGK+YEIKGCAGTGGF
Sbjct: 311 KDLLSKINPKILKYEGYHSSSKAYSRKMALNSLRNSSRNKTIEIGGKRYEIKGCAGTGGF 370

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300
           AQVYK+Y+D NP+DVVALKIQTPPFPWEFY+YRQLDQ VT+KERSSFGCAH MY YSDYS
Sbjct: 371 AQVYKAYVDGNPEDVVALKIQTPPFPWEFYMYRQLDQHVTMKERSSFGCAHAMYHYSDYS 430

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLH  GIIHGDFKPDNL
Sbjct: 431 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHGFGIIHGDFKPDNL 490

Query: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420
           LIRYAR  LTEDGFRERSGPW DQGLCLVDWGRGIDL LFPENVEF+GDCRTSGFRCTEM
Sbjct: 491 LIRYARDNLTEDGFRERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCTEM 550

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480
           LENKPWKYQVDIYG CVVVHTMLHGSYMDV+KK SS+G F+YLPKTSFKRYWN+ELWKNL
Sbjct: 551 LENKPWKYQVDIYGLCVVVHTMLHGSYMDVDKKASSNGGFIYLPKTSFKRYWNIELWKNL 610

Query: 481 FTKLLNIKLGDYNMELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 532
           FTKLLNI  GDYN +LLQSIR SFQDYMCSNP LIRKLKELL KQRASLCTA
Sbjct: 611 FTKLLNITPGDYNQKLLQSIRVSFQDYMCSNPHLIRKLKELLVKQRASLCTA 661

[Close]

BLAST of Csor.00g232180 vs. TAIR 10
Match: AT2G20635.1 (ATP binding;protein kinases;protein serine/threonine kinases )

HSP 1 Score: 671.8 bits (1732), Expect = 4.6e-193
Identity = 309/518 (59.65%), Postives = 402/518 (77.61%), Query Frame = 0


Query: 16  SAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTVKYKNDVRFLKIWFLS 75
           +A DPL PWL  IK+++E+  +G  SG  L  L+ DCI T+    +Y+ND+RFLKIWFL 
Sbjct: 8   AAGDPLFPWLMEIKNSMEDLYAGKNSGYDLDKLLFDCISTYKKDSRYRNDLRFLKIWFLY 67

Query: 76  IGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANMIYELGLLRNAEPLEW 135
           +  REDFE  ++E+ E EIC G+SLLY WYA+FLE KG    AN +Y+ GL R AEP + 
Sbjct: 68  LEGREDFERVYREIEETEICKGHSLLYEWYAIFLEVKGLWRRANSVYQTGLSRKAEPFDR 127

Query: 136 LKKAKVLFIDRMSELVNTHSM-KLNDGGESTKFVESYVNPWSSSVMKDLLSKINLKILKY 195
           LK+A  LF+ R+S+     S+ K+ D  ++T     +VNPW +S +  L+ KI  +++KY
Sbjct: 128 LKEAHSLFLQRISKRTKASSLEKVGDDAQATDLETGFVNPWETSTVNGLIHKIKPQLVKY 187

Query: 196 EGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDD 255
           +GYH S K +P K  L+SL+N SRNK+IEIGG+KY++KGCAG GGFAQV+K+++DSNPD+
Sbjct: 188 DGYHVSNKVFPGKANLSSLQNYSRNKIIEIGGRKYQMKGCAGQGGFAQVFKAFIDSNPDE 247

Query: 256 VVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYSILICDYLAHGTLQD 315
           VVALK+Q PPFPWEF++YRQLD R+   +RSSFG A  +++YSDYSIL+CDYL+HGTLQD
Sbjct: 248 VVALKVQKPPFPWEFHMYRQLDCRIPDSQRSSFGLAQRVHVYSDYSILVCDYLSHGTLQD 307

Query: 316 AINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNLLIRYARGKLTEDGF 375
            IN YVV+GK+MEEVLC+YYTIEML MLETLH++GIIHGDFKPDNLLIRY    LTE GF
Sbjct: 308 VINSYVVVGKSMEEVLCMYYTIEMLNMLETLHSVGIIHGDFKPDNLLIRYPPENLTETGF 367

Query: 376 RERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEMLENKPWKYQVDIYG 435
            E++G W+ +GLCLVDWGRGIDL LFP   EF GDCRTSGFRC EM E+KPWK+QVD YG
Sbjct: 368 HEKTGSWSKKGLCLVDWGRGIDLSLFPRTTEFTGDCRTSGFRCVEMKEDKPWKFQVDTYG 427

Query: 436 FCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNLFTKLLNIKLGDYNM 495
            CV+VH MLH  YM++EKK S DG ++ +P+TSFKRYW V+LWK LFTKLLN +  + + 
Sbjct: 428 LCVIVHMMLHNVYMEIEKKQSLDGGYINMPRTSFKRYWKVDLWKELFTKLLNRETCEDDT 487

Query: 496 ELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 533
           E L+++R+S ++Y+CS+P+L++KL ELLAKQR SLC++
Sbjct: 488 ETLRNLRKSMEEYICSDPKLMKKLNELLAKQRISLCSS 525

[Close]

BLAST of Csor.00g232180 vs. TAIR 10
Match: AT2G33560.1 (BUB1-related (BUB1: budding uninhibited by benzymidazol 1) )

HSP 1 Score: 79.7 bits (195), Expect = 7.7e-15
Identity = 53/187 (28.34%), Postives = 91/187 (48.66%), Query Frame = 0


Query: 19  DPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTVKYKNDVRFLKIWFLSIGM 78
           DPL PW+  IK   E    G     GL  +   C++ F  + +YK+D+R+LK+W      
Sbjct: 80  DPLSPWIECIKWVQEAFPPGGECS-GLLVIYEQCVRKFWHSERYKDDLRYLKVWLEYAEH 139

Query: 79  REDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANMIYELGLLRNAEPLEWLKK 138
             D E  +K L   EI   +++ YI YA+ +E K ++  AN I+ LG+ R+A+P+E L  
Sbjct: 140 CADAEVIYKFLEVNEIGKTHAVYYIAYALHIEFKNKVKTANEIFNLGISRDAKPVEKLND 199

Query: 139 AKVLFIDRMSELVNTHSMKLNDGGE-STKFVESYVNPWSSSVMKDLLSKINLKILKYEGY 198
           A   F+ R     NT   +  +  +  ++   + ++   ++  +  L   N +  K +  
Sbjct: 200 AYKKFMVRTMRRSNTADEEPKENNDLPSRSFGTLLSRGDNNARRQALGSSNPQAKKLKPN 259

Query: 199 HSSIKPY 205
            SS  P+
Sbjct: 260 QSSKTPF 265

[Close]

BLAST of Csor.00g232180 vs. TAIR 10
Match: AT2G33560.2 (BUB1-related (BUB1: budding uninhibited by benzymidazol 1) )

HSP 1 Score: 79.3 bits (194), Expect = 1.0e-14
Identity = 47/135 (34.81%), Postives = 71/135 (52.59%), Query Frame = 0


Query: 19  DPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTVKYKNDVRFLKIWFLSIGM 78
           DPL PW+  IK   E    G     GL  +   C++ F  + +YK+D+R+LK+W      
Sbjct: 80  DPLSPWIECIKWVQEAFPPGGECS-GLLVIYEQCVRKFWHSERYKDDLRYLKVWLEYAEH 139

Query: 79  REDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANMIYELGLLRNAEPLEWLKK 138
             D E  +K L   EI   +++ YI YA+ +E K ++  AN I+ LG+ R+A+P+E L  
Sbjct: 140 CADAEVIYKFLEVNEIGKTHAVYYIAYALHIEFKNKVKTANEIFNLGISRDAKPVEKLND 199

Query: 139 AKVLFIDRMSELVNT 154
           A   F+ R     NT
Sbjct: 200 AYKKFMVRTMRRSNT 213

[Close]

BLAST of Csor.00g232180 vs. TAIR 10
Match: AT5G05510.1 (Mad3/BUB1 homology region 1 )

HSP 1 Score: 75.5 bits (184), Expect = 1.5e-13
Identity = 39/132 (29.55%), Postives = 67/132 (50.76%), Query Frame = 0


Query: 16  SAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTVKYKNDVRFLKIWFLS 75
           S +D LLPW+  +K  ++ S    +    L   +  C ++F    +YKND R++++W   
Sbjct: 23  SGKDHLLPWIRGVK-KMKESLPSQILNEKLPRFLQKCAESFESDKRYKNDSRYIRVWLQL 82

Query: 76  IGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANMIYELGLLRNAEPLEW 135
           +   +D     + +  + I    SL Y  YA+  E   R  DA  +Y LG+   AEP++ 
Sbjct: 83  MDFVDDPRALLRTMEAKSIGTKRSLFYQAYALHYEKMKRFEDAEKMYRLGVQNLAEPMDE 142

Query: 136 LKKAKVLFIDRM 148
           L+K+ + F+ RM
Sbjct: 143 LQKSYLQFVTRM 153

[Close]

BLAST of Csor.00g232180 vs. TAIR 10
Match: AT3G03940.1 (Protein kinase family protein )

HSP 1 Score: 52.8 bits (125), Expect = 1.0e-06
Identity = 44/158 (27.85%), Postives = 66/158 (41.77%), Query Frame = 0


Query: 222 IEIGGKK-YEIKGCAGTGGFAQVYKSYLDSNPDD-------VVALKIQ---------TPP 281
           +++G    Y+ +   G GGF QVY     S   D        VALK++          PP
Sbjct: 132 VQVGNSPVYKTERKLGKGGFGQVYVGRRVSGGSDRIGADAIEVALKLEHRNSKGCNFGPP 191

Query: 282 FPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYSILICDYLAHGTLQDAINLYVVLGK 341
           + W+ Y         T+         H      D+ IL+ D L   +L D  N    L +
Sbjct: 192 YEWQVY--------NTLNSCYGIPAVHHKGRQGDFYILVMDMLG-PSLWDVWN---SLAQ 251

Query: 342 TMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNLLI 363
           +M   +     +E + +LE LH  G +HGD KP+N L+
Sbjct: 252 SMSPNMVACIAVEAISILEKLHMKGFVHGDVKPENFLL 277

The following BLAST results are available for this feature:

BLAST of Csor.00g232180 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4IVI0	6.5e-192	59.65	Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Arabidopsis thaliana ...	[more]
O43683	1.2e-47	33.71	Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Homo sapiens OX=9606 ...	[more]
O08901	3.3e-47	32.20	Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Mus musculus OX=10090...	[more]
P41695	3.7e-46	32.33	Checkpoint serine/threonine-protein kinase BUB1 OS=Saccharomyces cerevisiae (str...	[more]
O94751	3.2e-42	35.14	Checkpoint serine/threonine-protein kinase bub1 OS=Schizosaccharomyces pombe (st...	[more]

BLAST of Csor.00g232180 vs. NCBI nr


Match Name	Identity	Description
KAG6589257.1	100.00	Mitotic checkpoint serine/threonine-protein kinase BUB1, partial [Cucurbita argy...	[more]
XP_022930700.1	99.81	mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucurbita moschata]	[more]
KAG7022957.1	99.25	Mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucurbita argyrosperma ...	[more]
XP_022989217.1	98.31	mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucurbita maxima]	[more]
XP_023531077.1	98.31	mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucurbita pepo subsp. p...	[more]

BLAST of Csor.00g232180 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1EXJ7	99.81	mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Cucurbita moschata OX...	[more]
A0A6J1JF74	98.31	mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Cucurbita maxima OX=3...	[more]
A0A6J1D2B6	87.59	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X3 OS=Momordica ...	[more]
A0A6J1D1C8	87.59	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1 OS=Momordica ...	[more]
A0A6J1D2I5	87.59	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2 OS=Momordica ...	[more]

BLAST of Csor.00g232180 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G20635.1	4.6e-193	59.65	ATP binding;protein kinases;protein serine/threonine kinases	[more]
AT2G33560.1	7.7e-15	28.34	BUB1-related (BUB1: budding uninhibited by benzymidazol 1)	[more]
AT2G33560.2	1.0e-14	34.81	BUB1-related (BUB1: budding uninhibited by benzymidazol 1)	[more]
AT5G05510.1	1.5e-13	29.55	Mad3/BUB1 homology region 1	[more]
AT3G03940.1	1.0e-06	27.85	Protein kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013212	Mad3/Bub1 homology region 1	SMART	SM00777	mad3_bub1_i	coord: 4..126 e-value: 5.3E-10 score: 42.7
IPR013212	Mad3/Bub1 homology region 1	PFAM	PF08311	Mad3_BUB1_I	coord: 10..126 e-value: 8.3E-22 score: 77.5
IPR013212	Mad3/Bub1 homology region 1	PROSITE	PS51489	BUB1_N	coord: 6..164 score: 31.958305
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 229..497 e-value: 6.7E-9 score: -7.7
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 230..447 e-value: 1.7E-15 score: 57.2
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 229..532 score: 17.234306
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 227..529 e-value: 3.4E-98 score: 329.9
None	No IPR available	GENE3D	1.25.40.430		coord: 7..160 e-value: 2.0E-33 score: 117.3
None	No IPR available	PANTHER	PTHR14030:SF4	BUB1 KINASE, ISOFORM A-RELATED	coord: 15..148 coord: 169..531
IPR015661	Mitotic spindle checkpoint protein Bub1/Mad3	PANTHER	PTHR14030	MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1	coord: 169..531
IPR015661	Mitotic spindle checkpoint protein Bub1/Mad3	PANTHER	PTHR14030	MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1	coord: 15..148
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 350..362
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 226..455

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g232180.m01	Csor.00g232180.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032147	activation of protein kinase activity
biological_process	GO:0051754	meiotic sister chromatid cohesion, centromeric
biological_process	GO:0007094	mitotic spindle assembly checkpoint signaling
biological_process	GO:0060236	regulation of mitotic spindle organization
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0000776	kinetochore
cellular_component	GO:0005874	microtubule
cellular_component	GO:0005634	nucleus
cellular_component	GO:0005819	spindle
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity