Csor.00g227030 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g227030
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionaspartic proteinase Asp1-like isoform X2
LocationCsor_Chr09: 3976500 .. 3979646 (-)
RNA-Seq ExpressionCsor.00g227030
SyntenyCsor.00g227030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGATAAGAAATCGAGTCTTGTTTCAGGTTCACTAGAGATCCGTATGCCGGTTCTGTTGTTTGTCTCGATTCTCTTTGCGATTTTCGCCGTTTCCTTGTCTGACAAGTTTCTCACCGCCGACTGGGAGCAGTTGAGATCCGTTGGGTTTGGTCCGTCGATTTTGTTTCCTGTTAAAGGAAATGTTTATCCTCTAGGGTTTGTGTTTTGTGTTTTGATTTTCTTGTATTGTTTGGATTATTGAAATTTCCTTGATTATGTGTTGGAATCTGCAGTCATTTTACGGTTTCTGTCAAGATTGGTAACCCGCCGAAGGCTTTTGAACTGGATATTGACACTGGGAGTGATCTCACTTGGGTTCAATGCGATGTTCAGTGTATTGGTTGCACTCTGGTATGTTGGATCTTGATCGATTTTGACTGGATTGTTGTGTTTGTTACCAAGTTCTAGCGTAATTCTGTGTTATTCATTACTATTGTAGCCCCGTGATATGCTCTATAAACCGCATAATAACGCTGTGAGTCGTGAAGACCCATTGTGTGCGGCGCTTTCTTCGCCAGGCAATGCCCCTTGCAAGAAATCAAATGATCAATGTGACTACGAGGTCGAGTATGCTGACCATGGATCATCCATTGGTGTCTTGGTCAAAGATCTTGCTCCCATGCGACTCACGAATGGCCAACTCATCAATCCCAATTTGGGCTTTGGGTTTGTATACAATATATCAGTCTCCTTTGACAATTCTGCTTTATTACTGCCTTTTCATTGTTCGTGTCTTGTTTTTAAATATTATGCTGGTTTCTAGTAGTTTTTAGTTGTGCCTTTTCATTTTCTAAAATCATGATAGAATTCATGACTTAGATTGTGAAAATGTTTTAAAATGAAGATAATAAAACAAAGAATACTTAACTTTATTTCTAGAAACAAAAAGTTTAGGCCCTTCTTGAAATATGCTTTGTGTTCTTCCTCATTTATATACAAGATTTTTCATTTCTTTATTTAAATAAAAAACACTCTAGTTCTTAGTTTATGAGATTTCACATCGATTGGAGAGAAAAACAAGACATTTTTTTATAAGGGTGTGAAAACCTTTTCTTAGCGCACACGTTTTAAAAAACTTGAGGACAATATTTGTTTCGGGTGTACTAACTACTTTTTTGGGTTTTAAAGAAATGGTTAAAAAGTAGATCGTAAACAAAGGAACTTTTGATTTTGAGTGGAAGTGGCAGTAGTGTTTGTAAGTAAATTATGAAAGGGTTATAAAAGAGGCCAAAAACATCTTTTTGAGTGATTACTTAGTTTGGCCAGCCTAACACAGTACTACTTTTTGGCCTTTGTTCTTGGAAACTTAATACCAATCTTCCTTTCCTCTGATTCCTGTTGCCTGCAGTTGTGGATATGATCAGGACGATGGTGGCATGCAACCGCCTCCTTCAGTAGCTGGTGTTCTTGGCCTTAGTAGTAGTAAAGCAACCATTGTATCACAGCTAAGTGACCTCGGCCATGTAAGCAATGTCATTGGACATTGCCTGGCCGGACTTGGGGGAGGATTTCTATTCTTTGGAGGAGGCCTCGTCCCATCTTCCGGGATGTCGTGGACACCGATATTACGCAATTCAGAAGGGTCAGATAAATTTTGACATTGATCATTTGCTTCTTATACCTCCTCCATGTAGTTGACTTGGCATGTATTACTATTTGATCTCATTCATTCTCAGGAAGTACTCATCTGGACCTGCAGAGGTCTACTTTGGTGGGAAGGCTGGTGGAATTAGGGGCCTTACCCTGACTTTTGACAGTGGAAGCTCTTACACTTACTTCAACTCCCAAGTCTATGGAGCTGTAGAGAAACTGGTGAGAATATGGGAACAAGAACTTCTTTTATAGTTATAAATGTCTTGATTAGAACGATATCATTAGAAAAATAATGATCTGTCTTGACAATGATATCCCAGTTGAAGAATGATTTGAAAGGACAACCTCTAAAACTTGCTCCTGATGACAAAACCCTTGAACTGTGCTGGAAAGGCCCAAAAGCTTTCAAATCTGTGAGTGATGTGAGAGACTTCTTCAAGCCCCTGGCCTTAAACTTTAAGAACTCCAAGAACGCTCAATTTCAAATACCTCCTGAAGCTTATCTCATAATCAGCGTAAGTTCCTCAAACACCTCTCATTTATAACCCACAAGGGGTCGTGTCTTTCATTCAATGGATCGCTTAGCTCTATTTCTCCGTCAATCCCCTTTCTTTTTTCATGCTTTTCATTCATTTTTAGTAATGCTTTTTAGCTTTTCTTTTCTGATGAATGATATGTTAGATGAACACGATTCTCCACAATGATATGATATTGTCCACTTTGAACATTAGCTCTCATAGTATTCCTTAATTATAAACCCACGGTCATTCCCTAAATTAGTCGATGTGGGACTTTCATCCAACACCTCTCCTCGAACAAAGTATGCCTCCCCTTAATCGAGGCTCGACTTCTTTTCTTTTGGAGTCTTAGTCATTTTTTACTATGCCTTTGAGGCTCACAATACTAAGTTTAGGGCATGGCTCTGGTACCATGTTAGATCAACACGACTCTCCACAATGGTATGATATTGTCCACTTTGAGCATAACCTCTCGTGGCTTTGCTTTGGGTTTCTCCAAAAAGTCTTCATACCAATGGAGATAGTATTCCTTAATTATAAACCCATGATCATTCCCTAAATTAGCCGAGGTGAGACTTTCATCCAACATGATATTGAGGAACTGACGGTACGAATCTCTTCATTCATTTACAGGAATTTGGTAATGTGTGCTTGGGGATTCTGGATGGAACCAAAGAAGGATTGGGGAATGTCAACCTGATTGGAGGTAAGATTAGAGAGGTGTTGGGTGATGAACAAAGAACCATTTCAGATTCTTTTCTTTTACATGCTTTCTTCCAACCTTCCTCAAATTCTTGTACAGATATTTCTATGCTCAATAAGATTGTAGTCTATGACAACCAGAGACGGCAGATTGGCTGGGCTCCTCAAAACTGCAATCCACCTCCCAGAAATGAAGCTGCATATGGTATGTGCCATTTTCATTTATGAACATCAACTTTGCTTTTCATCATCTTATTTTAAGAAATCTTCCCATTTTAGGTGATCAATGA

mRNA sequence

ATGGAGGATAAGAAATCGAGTCTTGTTTCAGGTTCACTAGAGATCCGTATGCCGGTTCTGTTGTTTGTCTCGATTCTCTTTGCGATTTTCGCCGTTTCCTTGTCTGACAAGTTTCTCACCGCCGACTGGGAGCAGTTGAGATCCGTTGGGTTTGGTCCGTCGATTTTGTTTCCTGTTAAAGGAAATGTTTATCCTCTAGGTCATTTTACGGTTTCTGTCAAGATTGGTAACCCGCCGAAGGCTTTTGAACTGGATATTGACACTGGGAGTGATCTCACTTGGGTTCAATGCGATGTTCAGTGTATTGGTTGCACTCTGCCCCGTGATATGCTCTATAAACCGCATAATAACGCTGTGAGTCGTGAAGACCCATTGTGTGCGGCGCTTTCTTCGCCAGGCAATGCCCCTTGCAAGAAATCAAATGATCAATGTGACTACGAGGTCGAGTATGCTGACCATGGATCATCCATTGGTGTCTTGGTCAAAGATCTTGCTCCCATGCGACTCACGAATGGCCAACTCATCAATCCCAATTTGGGCTTTGGTTGTGGATATGATCAGGACGATGGTGGCATGCAACCGCCTCCTTCAGTAGCTGGTGTTCTTGGCCTTAGTAGTAGTAAAGCAACCATTGTATCACAGCTAAGTGACCTCGGCCATGTAAGCAATGTCATTGGACATTGCCTGGCCGGACTTGGGGGAGGATTTCTATTCTTTGGAGGAGGCCTCGTCCCATCTTCCGGGATGTCGTGGACACCGATATTACGCAATTCAGAAGGGAAGTACTCATCTGGACCTGCAGAGGTCTACTTTGGTGGGAAGGCTGGTGGAATTAGGGGCCTTACCCTGACTTTTGACAGTGGAAGCTCTTACACTTACTTCAACTCCCAAGTCTATGGAGCTGTAGAGAAACTGTTGAAGAATGATTTGAAAGGACAACCTCTAAAACTTGCTCCTGATGACAAAACCCTTGAACTGTGCTGGAAAGGCCCAAAAGCTTTCAAATCTGTGAGTGATGTGAGAGACTTCTTCAAGCCCCTGGCCTTAAACTTTAAGAACTCCAAGAACGCTCAATTTCAAATACCTCCTGAAGCTTATCTCATAATCAGCGAATTTGGTAATGTGTGCTTGGGGATTCTGGATGGAACCAAAGAAGGATTGGGGAATGTCAACCTGATTGGAGATATTTCTATGCTCAATAAGATTGTAGTCTATGACAACCAGAGACGGCAGATTGGCTGGGCTCCTCAAAACTGCAATCCACCTCCCAGAAATGAAGCTGCATATGGTGATCAATGA

Coding sequence (CDS)

ATGGAGGATAAGAAATCGAGTCTTGTTTCAGGTTCACTAGAGATCCGTATGCCGGTTCTGTTGTTTGTCTCGATTCTCTTTGCGATTTTCGCCGTTTCCTTGTCTGACAAGTTTCTCACCGCCGACTGGGAGCAGTTGAGATCCGTTGGGTTTGGTCCGTCGATTTTGTTTCCTGTTAAAGGAAATGTTTATCCTCTAGGTCATTTTACGGTTTCTGTCAAGATTGGTAACCCGCCGAAGGCTTTTGAACTGGATATTGACACTGGGAGTGATCTCACTTGGGTTCAATGCGATGTTCAGTGTATTGGTTGCACTCTGCCCCGTGATATGCTCTATAAACCGCATAATAACGCTGTGAGTCGTGAAGACCCATTGTGTGCGGCGCTTTCTTCGCCAGGCAATGCCCCTTGCAAGAAATCAAATGATCAATGTGACTACGAGGTCGAGTATGCTGACCATGGATCATCCATTGGTGTCTTGGTCAAAGATCTTGCTCCCATGCGACTCACGAATGGCCAACTCATCAATCCCAATTTGGGCTTTGGTTGTGGATATGATCAGGACGATGGTGGCATGCAACCGCCTCCTTCAGTAGCTGGTGTTCTTGGCCTTAGTAGTAGTAAAGCAACCATTGTATCACAGCTAAGTGACCTCGGCCATGTAAGCAATGTCATTGGACATTGCCTGGCCGGACTTGGGGGAGGATTTCTATTCTTTGGAGGAGGCCTCGTCCCATCTTCCGGGATGTCGTGGACACCGATATTACGCAATTCAGAAGGGAAGTACTCATCTGGACCTGCAGAGGTCTACTTTGGTGGGAAGGCTGGTGGAATTAGGGGCCTTACCCTGACTTTTGACAGTGGAAGCTCTTACACTTACTTCAACTCCCAAGTCTATGGAGCTGTAGAGAAACTGTTGAAGAATGATTTGAAAGGACAACCTCTAAAACTTGCTCCTGATGACAAAACCCTTGAACTGTGCTGGAAAGGCCCAAAAGCTTTCAAATCTGTGAGTGATGTGAGAGACTTCTTCAAGCCCCTGGCCTTAAACTTTAAGAACTCCAAGAACGCTCAATTTCAAATACCTCCTGAAGCTTATCTCATAATCAGCGAATTTGGTAATGTGTGCTTGGGGATTCTGGATGGAACCAAAGAAGGATTGGGGAATGTCAACCTGATTGGAGATATTTCTATGCTCAATAAGATTGTAGTCTATGACAACCAGAGACGGCAGATTGGCTGGGCTCCTCAAAACTGCAATCCACCTCCCAGAAATGAAGCTGCATATGGTGATCAATGA

Protein sequence

MEDKKSSLVSGSLEIRMPVLLFVSILFAIFAVSLSDKFLTADWEQLRSVGFGPSILFPVKGNVYPLGHFTVSVKIGNPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVSREDPLCAALSSPGNAPCKKSNDQCDYEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLGFGCGYDQDDGGMQPPPSVAGVLGLSSSKATIVSQLSDLGHVSNVIGHCLAGLGGGFLFFGGGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYGAVEKLLKNDLKGQPLKLAPDDKTLELCWKGPKAFKSVSDVRDFFKPLALNFKNSKNAQFQIPPEAYLIISEFGNVCLGILDGTKEGLGNVNLIGDISMLNKIVVYDNQRRQIGWAPQNCNPPPRNEAAYGDQ
Homology
BLAST of Csor.00g227030 vs. ExPASy Swiss-Prot
Match: Q0IU52 (Aspartic proteinase Asp1 OS=Oryza sativa subsp. japonica OX=39947 GN=ASP1 PE=2 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.7e-81
Identity = 165/387 (42.64%), Postives = 239/387 (61.76%), Query Frame = 0

Query: 54  SILFPVKGNVYPLGHFTVSVKIGNPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYK 113
           +++  + GNVYP+GHF +++ IG+P K++ LDIDTGS LTW+QCD  C  C +   +LYK
Sbjct: 23  AVVLELHGNVYPIGHFFITMNIGDPAKSYFLDIDTGSTLTWLQCDAPCTNCNIVPHVLYK 82

Query: 114 P-HNNAVSREDPLCAALSSPGNAPCK-KSNDQCDYEVEYADHGSSIGVLVKDLAPMRLTN 173
           P     V+  D LC  L +    P +  S  QCDY ++Y D  SS+GVLV D   +  +N
Sbjct: 83  PTPKKLVTCADSLCTDLYTDLGKPKRCGSQKQCDYVIQYVD-SSSMGVLVIDRFSLSASN 142

Query: 174 GQLINP-NLGFGCGYDQDDGGMQPPPSVAGVLGLSSSKATIVSQLSDLGHVS-NVIGHCL 233
           G   NP  + FGCGYDQ       P  V  +LGLS  K T++SQL   G ++ +V+GHC+
Sbjct: 143 G--TNPTTIAFGCGYDQGKKNRNVPIPVDSILGLSRGKVTLLSQLKSQGVITKHVLGHCI 202

Query: 234 AGLGGGFLFFGGGLVPSSGMSWTPILRNSEGKYSSGPAEVYF--GGKAGGIRGLTLTFDS 293
           +  GGGFLFFG   VP+SG++WTP+ R  +  YS G   ++F    KA     + + FDS
Sbjct: 203 SSKGGGFLFFGDAQVPTSGVTWTPMNREHK-YYSPGHGTLHFDSNSKAISAAPMAVIFDS 262

Query: 294 GSSYTYFNSQVYGAVEKLLKNDLKGQ---PLKLAPDDKTLELCWKGPKAFKSVSDVRDFF 353
           G++YTYF +Q Y A   ++K+ L  +     ++   D+ L +CWKG     ++ +V+  F
Sbjct: 263 GATYTYFAAQPYQATLSVVKSTLNSECKFLTEVTEKDRALTVCWKGKDKIVTIDEVKKCF 322

Query: 354 KPLALNFKN-SKNAQFQIPPEAYLIISEFGNVCLGILDGTKE--GLGNVNLIGDISMLNK 413
           + L+L F +  K A  +IPPE YLIIS+ G+VCLGILDG+KE   L   NLIG I+ML++
Sbjct: 323 RSLSLEFADGDKKATLEIPPEHYLIISQEGHVCLGILDGSKEHLSLAGTNLIGGITMLDQ 382

Query: 414 IVVYDNQRRQIGWAPQNCNPPPRNEAA 429
           +V+YD++R  +GW    C+  PR+E+A
Sbjct: 383 MVIYDSERSLLGWVNYQCDRIPRSESA 405

BLAST of Csor.00g227030 vs. ExPASy Swiss-Prot
Match: A2ZC67 (Aspartic proteinase Asp1 OS=Oryza sativa subsp. indica OX=39946 GN=ASP1 PE=2 SV=2)

HSP 1 Score: 288.9 bits (738), Expect = 9.6e-77
Identity = 160/387 (41.34%), Postives = 233/387 (60.21%), Query Frame = 0

Query: 54  SILFPVKGNVYPLGHFTVSVKIGNPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYK 113
           +++  + GNVYP+GHF V++ IG+P K + LDIDTGS LTW+QCD  CI C      LYK
Sbjct: 23  AVVLELHGNVYPIGHFFVTMNIGDPAKPYFLDIDTGSTLTWLQCDYPCINCNKVPHGLYK 82

Query: 114 PH-NNAVSREDPLCAALSSPGNAPCK-KSNDQCDYEVEYADHGSSIGVLVKDLAPMRLTN 173
           P    AV   +  CA L +    P K    +QC Y ++Y   GSSIGVL+ D   +  +N
Sbjct: 83  PELKYAVKCTEQRCADLYADLRKPMKCGPKNQCHYGIQYVG-GSSIGVLIVDSFSLPASN 142

Query: 174 GQLINP-NLGFGCGYDQDDGGMQPPPSVAGVLGLSSSKATIVSQLSDLGHVS-NVIGHCL 233
           G   NP ++ FGCGY+Q       P  V G+LGL   K T++SQL   G ++ +V+GHC+
Sbjct: 143 G--TNPTSIAFGCGYNQGKNNHNVPTPVNGILGLGRGKVTLLSQLKSQGVITKHVLGHCI 202

Query: 234 AGLGGGFLFFGGGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIRG--LTLTFDS 293
           +  G GFLFFG   VP+SG++W+P+ R  +  YS     + F   +  I    + + FDS
Sbjct: 203 SSKGKGFLFFGDAKVPTSGVTWSPMNREHK-HYSPRQGTLQFNSNSKPISAAPMEVIFDS 262

Query: 294 GSSYTYFNSQVYGAVEKLLKNDLKGQ---PLKLAPDDKTLELCWKGPKAFKSVSDVRDFF 353
           G++YTYF  Q Y A   ++K+ L  +     ++   D+ L +CWKG    +++ +V+  F
Sbjct: 263 GATYTYFALQPYHATLSVVKSTLSKECKFLTEVKEKDRALTVCWKGKDKIRTIDEVKKCF 322

Query: 354 KPLALNFKN-SKNAQFQIPPEAYLIISEFGNVCLGILDGTKE--GLGNVNLIGDISMLNK 413
           + L+L F +  K A  +IPPE YLIIS+ G+VCLGILDG+KE   L   NLIG I+ML++
Sbjct: 323 RSLSLKFADGDKKATLEIPPEHYLIISQEGHVCLGILDGSKEHPSLAGTNLIGGITMLDQ 382

Query: 414 IVVYDNQRRQIGWAPQNCNPPPRNEAA 429
           +V+YD++R  +GW    C+  PR+ +A
Sbjct: 383 MVIYDSERSLLGWVNYQCDRIPRSASA 405

BLAST of Csor.00g227030 vs. ExPASy Swiss-Prot
Match: Q9M9A8 (Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 1.3e-76
Identity = 159/385 (41.30%), Postives = 221/385 (57.40%), Query Frame = 0

Query: 56  LFPVKGNVYPLGHFTVSVKIGNPP--KAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYK 115
           +FPV GNVYP G +   + +G P   + + LDIDTGS+LTW+QCD  C  C    + LYK
Sbjct: 190 IFPVGGNVYPDGLYYTRILVGKPEDGQYYHLDIDTGSELTWIQCDAPCTSCAKGANQLYK 249

Query: 116 PH-NNAVSREDPLCAALSSPGNAPCKKSNDQCDYEVEYADHGSSIGVLVKDLAPMRLTNG 175
           P  +N V   +  C  +         ++  QCDYE+EYADH  S+GVL KD   ++L NG
Sbjct: 250 PRKDNLVRSSEAFCVEVQRNQLTEHCENCHQCDYEIEYADHSYSMGVLTKDKFHLKLHNG 309

Query: 176 QLINPNLGFGCGYDQDDGGMQPPPSVAGVLGLSSSKATIVSQLSDLGHVSNVIGHCLAG- 235
            L   ++ FGCGYDQ    +       G+LGLS +K ++ SQL+  G +SNV+GHCLA  
Sbjct: 310 SLAESDIVFGCGYDQQGLLLNTLLKTDGILGLSRAKISLPSQLASRGIISNVVGHCLASD 369

Query: 236 -LGGGFLFFGGGLVPSSGMSWTPILRNSE--------GKYSSGPAEVYFGGKAGGIRGLT 295
             G G++F G  LVPS GM+W P+L +S          K S G   +   G+ G +    
Sbjct: 370 LNGEGYIFMGSDLVPSHGMTWVPMLHDSRLDAYQMQVTKMSYGQGMLSLDGENGRVG--K 429

Query: 296 LTFDSGSSYTYFNSQVYGAVEKLLKNDLKGQPLKLAPDDKTLELCWKGPK--AFKSVSDV 355
           + FD+GSSYTYF +Q Y  +   L+ ++ G  L     D+TL +CW+      F S+SDV
Sbjct: 430 VLFDTGSSYTYFPNQAYSQLVTSLQ-EVSGLELTRDDSDETLPICWRAKTNFPFSSLSDV 489

Query: 356 RDFFKPLALNFKNS---KNAQFQIPPEAYLIISEFGNVCLGILDGTKEGLGNVNLIGDIS 415
           + FF+P+ L   +     + +  I PE YLIIS  GNVCLGILDG+    G+  ++GDIS
Sbjct: 490 KKFFRPITLQIGSKWLIISRKLLIQPEDYLIISNKGNVCLGILDGSSVHDGSTIILGDIS 549

Query: 416 MLNKIVVYDNQRRQIGWAPQNCNPP 423
           M   ++VYDN +R+IGW   +C  P
Sbjct: 550 MRGHLIVYDNVKRRIGWMKSDCVRP 571

BLAST of Csor.00g227030 vs. ExPASy Swiss-Prot
Match: Q9S9K4 (Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2)

HSP 1 Score: 151.4 bits (381), Expect = 2.4e-35
Identity = 113/396 (28.54%), Postives = 178/396 (44.95%), Query Frame = 0

Query: 54  SILFPVKGN--VYPLGHFTVSVKIGNPPKAFELDIDTGSDLTWVQCDVQCIGCTLPR--- 113
           SI  P+ G+  V  +G +   +K+G+PPK + + +DTGSD+ W+ C   C  C       
Sbjct: 57  SIDLPLGGDSRVDSVGLYFTKIKLGSPPKEYHVQVDTGSDILWINCK-PCPKCPTKTNLN 116

Query: 114 ------DMLYKPHNNAVSREDPLCAALSSPGNAPCKKSNDQCDYEVEYADHGSSIGVLVK 173
                 DM     +  V  +D  C+ +S   +  C+ +   C Y + YAD  +S G  ++
Sbjct: 117 FRLSLFDMNASSTSKKVGCDDDFCSFISQSDS--CQPALG-CSYHIVYADESTSDGKFIR 176

Query: 174 DLAPMRLTNGQLINPNLG----FGCGYDQDDGGMQPPPSVAGVLGLSSSKATIVSQLSDL 233
           D+  +    G L    LG    FGCG DQ         +V GV+G   S  +++SQL+  
Sbjct: 177 DMLTLEQVTGDLKTGPLGQEVVFGCGSDQSGQLGNGDSAVDGVMGFGQSNTSVLSQLAAT 236

Query: 234 GHVSNVIGHCLAGLGGGFLFFGGGLVPSSGMSWTPILRNS------------EGKYSSGP 293
           G    V  HCL  + GG + F  G+V S  +  TP++ N             +G     P
Sbjct: 237 GDAKRVFSHCLDNVKGGGI-FAVGVVDSPKVKTTPMVPNQMHYNVMLMGMDVDGTSLDLP 296

Query: 294 AEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYGAVEKLLKNDLKGQPLKLAPDDKTLEL 353
             +        +R      DSG++  YF   +Y   + L++  L  QP+KL   ++T + 
Sbjct: 297 RSI--------VRNGGTIVDSGTTLAYFPKVLY---DSLIETILARQPVKLHIVEETFQ- 356

Query: 354 CWKGPKAFKSVSDVRDFFKPLALNFKNSKNAQFQIPPEAYLIISEFGNVCLGILDG--TK 413
                  F   ++V + F P++  F++S   +  + P  YL   E    C G   G  T 
Sbjct: 357 ------CFSFSTNVDEAFPPVSFEFEDS--VKLTVYPHDYLFTLEEELYCFGWQAGGLTT 416

Query: 414 EGLGNVNLIGDISMLNKIVVYDNQRRQIGWAPQNCN 421
           +    V L+GD+ + NK+VVYD     IGWA  NC+
Sbjct: 417 DERSEVILLGDLVLSNKLVVYDLDNEVIGWADHNCS 427

BLAST of Csor.00g227030 vs. ExPASy Swiss-Prot
Match: Q4V3D2 (Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 4.2e-32
Identity = 110/383 (28.72%), Postives = 166/383 (43.34%), Query Frame = 0

Query: 66  LGHFTVSVKIGNPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDM-----LYKPHNNAVS 125
           +G +   +K+G+PPK + + +DTGSD+ WV C   C  C +  D+     LY    ++ S
Sbjct: 75  IGLYFTKIKLGSPPKEYYVQVDTGSDILWVNC-APCPKCPVKTDLGIPLSLYDSKTSSTS 134

Query: 126 R----EDPLCAALSSPGNAPCKKSNDQCDYEVEYADHGSSIGVLVKDLAPMRLTNGQLIN 185
           +    ED  C+ +        KK    C Y V Y D  +S G  +KD   +    G L  
Sbjct: 135 KNVGCEDDFCSFIMQSETCGAKK---PCSYHVVYGDGSTSDGDFIKDNITLEQVTGNLRT 194

Query: 186 PNLG----FGCGYDQDDGGMQPPPSVAGVLGLSSSKATIVSQLSDLGHVSNVIGHCLAGL 245
             L     FGCG +Q     Q   +V G++G   S  +I+SQL+  G    +  HCL  +
Sbjct: 195 APLAQEVVFGCGKNQSGQLGQTDSAVDGIMGFGQSNTSIISQLAAGGSTKRIFSHCLDNM 254

Query: 246 GGGFLFFGGGLVPSSGMSWTPILRNS-------EGKYSSG------PAEVYFGGKAGGIR 305
            GG + F  G V S  +  TPI+ N        +G    G      P+     G  G I 
Sbjct: 255 NGGGI-FAVGEVESPVVKTTPIVPNQVHYNVILKGMDVDGDPIDLPPSLASTNGDGGTI- 314

Query: 306 GLTLTFDSGSSYTYFNSQVYGAVEKLLKNDLKGQPLKLAPDDKTLELCWKGPKAFKSVSD 365
                 DSG++  Y    +Y +   L++     Q +KL    +T          F   S+
Sbjct: 315 -----IDSGTTLAYLPQNLYNS---LIEKITAKQQVKLHMVQETF-------ACFSFTSN 374

Query: 366 VRDFFKPLALNFKNSKNAQFQIPPEAYLIISEFGNVCLGILDG--TKEGLGNVNLIGDIS 421
               F  + L+F++S   +  + P  YL        C G   G  T +   +V L+GD+ 
Sbjct: 375 TDKAFPVVNLHFEDS--LKLSVYPHDYLFSLREDMYCFGWQSGGMTTQDGADVILLGDLV 434

BLAST of Csor.00g227030 vs. NCBI nr
Match: KAG6591879.1 (Aspartic proteinase Asp1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 884 bits (2283), Expect = 0.0
Identity = 432/432 (100.00%), Postives = 432/432 (100.00%), Query Frame = 0

Query: 1   MEDKKSSLVSGSLEIRMPVLLFVSILFAIFAVSLSDKFLTADWEQLRSVGFGPSILFPVK 60
           MEDKKSSLVSGSLEIRMPVLLFVSILFAIFAVSLSDKFLTADWEQLRSVGFGPSILFPVK
Sbjct: 1   MEDKKSSLVSGSLEIRMPVLLFVSILFAIFAVSLSDKFLTADWEQLRSVGFGPSILFPVK 60

Query: 61  GNVYPLGHFTVSVKIGNPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVS 120
           GNVYPLGHFTVSVKIGNPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVS
Sbjct: 61  GNVYPLGHFTVSVKIGNPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVS 120

Query: 121 REDPLCAALSSPGNAPCKKSNDQCDYEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG 180
           REDPLCAALSSPGNAPCKKSNDQCDYEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG
Sbjct: 121 REDPLCAALSSPGNAPCKKSNDQCDYEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG 180

Query: 181 FGCGYDQDDGGMQPPPSVAGVLGLSSSKATIVSQLSDLGHVSNVIGHCLAGLGGGFLFFG 240
           FGCGYDQDDGGMQPPPSVAGVLGLSSSKATIVSQLSDLGHVSNVIGHCLAGLGGGFLFFG
Sbjct: 181 FGCGYDQDDGGMQPPPSVAGVLGLSSSKATIVSQLSDLGHVSNVIGHCLAGLGGGFLFFG 240

Query: 241 GGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYG 300
           GGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYG
Sbjct: 241 GGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYG 300

Query: 301 AVEKLLKNDLKGQPLKLAPDDKTLELCWKGPKAFKSVSDVRDFFKPLALNFKNSKNAQFQ 360
           AVEKLLKNDLKGQPLKLAPDDKTLELCWKGPKAFKSVSDVRDFFKPLALNFKNSKNAQFQ
Sbjct: 301 AVEKLLKNDLKGQPLKLAPDDKTLELCWKGPKAFKSVSDVRDFFKPLALNFKNSKNAQFQ 360

Query: 361 IPPEAYLIISEFGNVCLGILDGTKEGLGNVNLIGDISMLNKIVVYDNQRRQIGWAPQNCN 420
           IPPEAYLIISEFGNVCLGILDGTKEGLGNVNLIGDISMLNKIVVYDNQRRQIGWAPQNCN
Sbjct: 361 IPPEAYLIISEFGNVCLGILDGTKEGLGNVNLIGDISMLNKIVVYDNQRRQIGWAPQNCN 420

Query: 421 PPPRNEAAYGDQ 432
           PPPRNEAAYGDQ
Sbjct: 421 PPPRNEAAYGDQ 432

BLAST of Csor.00g227030 vs. NCBI nr
Match: XP_022936138.1 (aspartic proteinase Asp1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 861 bits (2224), Expect = 3.56e-315
Identity = 421/432 (97.45%), Postives = 426/432 (98.61%), Query Frame = 0

Query: 1   MEDKKSSLVSGSLEIRMPVLLFVSILFAIFAVSLSDKFLTADWEQLRSVGFGPSILFPVK 60
           MEDKKSSLVSGSLEIRMPVLLFVSILFAIFAVSLSD+FLTADWEQLRSVGFG SILFPVK
Sbjct: 1   MEDKKSSLVSGSLEIRMPVLLFVSILFAIFAVSLSDQFLTADWEQLRSVGFGSSILFPVK 60

Query: 61  GNVYPLGHFTVSVKIGNPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVS 120
           GNVYPLGHFTVSV IGNPPKAFELDIDTGSDLTWVQCDVQC GCTLPRDMLYKPHNNAVS
Sbjct: 61  GNVYPLGHFTVSVTIGNPPKAFELDIDTGSDLTWVQCDVQCTGCTLPRDMLYKPHNNAVS 120

Query: 121 REDPLCAALSSPGNAPCKKSNDQCDYEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG 180
           REDPLCAALSSPGNAPCKKSNDQCDY+VEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG
Sbjct: 121 REDPLCAALSSPGNAPCKKSNDQCDYDVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG 180

Query: 181 FGCGYDQDDGGMQPPPSVAGVLGLSSSKATIVSQLSDLGHVSNVIGHCLAGLGGGFLFFG 240
           FGCGYDQD GGMQPPP VAGVLGLSSSKATIVSQL+DLGHVSNVIGHCLAGLGGGFLFFG
Sbjct: 181 FGCGYDQDYGGMQPPPLVAGVLGLSSSKATIVSQLNDLGHVSNVIGHCLAGLGGGFLFFG 240

Query: 241 GGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYG 300
           GGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGI+GLTLTFDSGSSYTYFNSQVYG
Sbjct: 241 GGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIKGLTLTFDSGSSYTYFNSQVYG 300

Query: 301 AVEKLLKNDLKGQPLKLAPDDKTLELCWKGPKAFKSVSDVRDFFKPLALNFKNSKNAQFQ 360
           AVEKLLKNDLKGQPLKLAPDDKTLELCWKG KAFKSVSDVRDFFKPLALNFKNSKNAQFQ
Sbjct: 301 AVEKLLKNDLKGQPLKLAPDDKTLELCWKGRKAFKSVSDVRDFFKPLALNFKNSKNAQFQ 360

Query: 361 IPPEAYLIISEFGNVCLGILDGTKEGLGNVNLIGDISMLNKIVVYDNQRRQIGWAPQNCN 420
           IPPEAYLIISEFGNVCLGILDGTKEGLGNVNLIGDISMLNKIVVYDNQRRQIGWAPQNCN
Sbjct: 361 IPPEAYLIISEFGNVCLGILDGTKEGLGNVNLIGDISMLNKIVVYDNQRRQIGWAPQNCN 420

Query: 421 PPPRNEAAYGDQ 432
           PPPRNEA+YGDQ
Sbjct: 421 PPPRNEASYGDQ 432

BLAST of Csor.00g227030 vs. NCBI nr
Match: XP_022976721.1 (aspartic proteinase Asp1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 859 bits (2219), Expect = 2.31e-314
Identity = 422/432 (97.69%), Postives = 423/432 (97.92%), Query Frame = 0

Query: 1   MEDKKSSLVSGSLEIRMPVLLFVSILFAIFAVSLSDKFLTADWEQLRSVGFGPSILFPVK 60
           MEDKKSS VSGSLEIRMPVLLFVSILFAIFAVSLSDKFLTADWEQLRSVGFG SILFPVK
Sbjct: 1   MEDKKSSFVSGSLEIRMPVLLFVSILFAIFAVSLSDKFLTADWEQLRSVGFGSSILFPVK 60

Query: 61  GNVYPLGHFTVSVKIGNPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVS 120
           GNVYPLGHFTVSVKIG PPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVS
Sbjct: 61  GNVYPLGHFTVSVKIGKPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVS 120

Query: 121 REDPLCAALSSPGNAPCKKSNDQCDYEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG 180
           REDPLCAALSSPGNAPCKKSNDQC YEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG
Sbjct: 121 REDPLCAALSSPGNAPCKKSNDQCVYEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG 180

Query: 181 FGCGYDQDDGGMQPPPSVAGVLGLSSSKATIVSQLSDLGHVSNVIGHCLAGLGGGFLFFG 240
           FGCGYDQDDGG QPPPSVAGVLGLS SKATIVSQLSDLGHVSNVIGHCLA LGGGFLFFG
Sbjct: 181 FGCGYDQDDGGTQPPPSVAGVLGLSCSKATIVSQLSDLGHVSNVIGHCLAELGGGFLFFG 240

Query: 241 GGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYG 300
            GLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYG
Sbjct: 241 AGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYG 300

Query: 301 AVEKLLKNDLKGQPLKLAPDDKTLELCWKGPKAFKSVSDVRDFFKPLALNFKNSKNAQFQ 360
           AVEKLLKNDLKGQPLKLAPDDKTLELCWKG KAFKSVSDVRDFFKPLALNFKNSKNAQFQ
Sbjct: 301 AVEKLLKNDLKGQPLKLAPDDKTLELCWKGRKAFKSVSDVRDFFKPLALNFKNSKNAQFQ 360

Query: 361 IPPEAYLIISEFGNVCLGILDGTKEGLGNVNLIGDISMLNKIVVYDNQRRQIGWAPQNCN 420
           IPPEAYLIISEFGNVCLGILDGTKEGLGNVNLIGDISMLNKIVVYDNQRRQIGWAPQNCN
Sbjct: 361 IPPEAYLIISEFGNVCLGILDGTKEGLGNVNLIGDISMLNKIVVYDNQRRQIGWAPQNCN 420

Query: 421 PPPRNEAAYGDQ 432
           PPPRNEA+YGDQ
Sbjct: 421 PPPRNEASYGDQ 432

BLAST of Csor.00g227030 vs. NCBI nr
Match: XP_022936137.1 (aspartic proteinase Asp1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 730 bits (1884), Expect = 2.91e-259
Identity = 360/370 (97.30%), Postives = 364/370 (98.38%), Query Frame = 0

Query: 1   MEDKKSSLVSGSLEIRMPVLLFVSILFAIFAVSLSDKFLTADWEQLRSVGFGPSILFPVK 60
           MEDKKSSLVSGSLEIRMPVLLFVSILFAIFAVSLSD+FLTADWEQLRSVGFG SILFPVK
Sbjct: 1   MEDKKSSLVSGSLEIRMPVLLFVSILFAIFAVSLSDQFLTADWEQLRSVGFGSSILFPVK 60

Query: 61  GNVYPLGHFTVSVKIGNPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVS 120
           GNVYPLGHFTVSV IGNPPKAFELDIDTGSDLTWVQCDVQC GCTLPRDMLYKPHNNAVS
Sbjct: 61  GNVYPLGHFTVSVTIGNPPKAFELDIDTGSDLTWVQCDVQCTGCTLPRDMLYKPHNNAVS 120

Query: 121 REDPLCAALSSPGNAPCKKSNDQCDYEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG 180
           REDPLCAALSSPGNAPCKKSNDQCDY+VEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG
Sbjct: 121 REDPLCAALSSPGNAPCKKSNDQCDYDVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG 180

Query: 181 FGCGYDQDDGGMQPPPSVAGVLGLSSSKATIVSQLSDLGHVSNVIGHCLAGLGGGFLFFG 240
           FGCGYDQD GGMQPPP VAGVLGLSSSKATIVSQL+DLGHVSNVIGHCLAGLGGGFLFFG
Sbjct: 181 FGCGYDQDYGGMQPPPLVAGVLGLSSSKATIVSQLNDLGHVSNVIGHCLAGLGGGFLFFG 240

Query: 241 GGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYG 300
           GGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGI+GLTLTFDSGSSYTYFNSQVYG
Sbjct: 241 GGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIKGLTLTFDSGSSYTYFNSQVYG 300

Query: 301 AVEKLLKNDLKGQPLKLAPDDKTLELCWKGPKAFKSVSDVRDFFKPLALNFKNSKNAQFQ 360
           AVEKLLKNDLKGQPLKLAPDDKTLELCWKG KAFKSVSDVRDFFKPLALNFKNSKNAQFQ
Sbjct: 301 AVEKLLKNDLKGQPLKLAPDDKTLELCWKGRKAFKSVSDVRDFFKPLALNFKNSKNAQFQ 360

Query: 361 IPPEAYLIIS 370
           IPPEAYLIIS
Sbjct: 361 IPPEAYLIIS 370

BLAST of Csor.00g227030 vs. NCBI nr
Match: XP_022976720.1 (aspartic proteinase Asp1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 728 bits (1879), Expect = 1.67e-258
Identity = 361/370 (97.57%), Postives = 361/370 (97.57%), Query Frame = 0

Query: 1   MEDKKSSLVSGSLEIRMPVLLFVSILFAIFAVSLSDKFLTADWEQLRSVGFGPSILFPVK 60
           MEDKKSS VSGSLEIRMPVLLFVSILFAIFAVSLSDKFLTADWEQLRSVGFG SILFPVK
Sbjct: 1   MEDKKSSFVSGSLEIRMPVLLFVSILFAIFAVSLSDKFLTADWEQLRSVGFGSSILFPVK 60

Query: 61  GNVYPLGHFTVSVKIGNPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVS 120
           GNVYPLGHFTVSVKIG PPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVS
Sbjct: 61  GNVYPLGHFTVSVKIGKPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVS 120

Query: 121 REDPLCAALSSPGNAPCKKSNDQCDYEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG 180
           REDPLCAALSSPGNAPCKKSNDQC YEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG
Sbjct: 121 REDPLCAALSSPGNAPCKKSNDQCVYEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG 180

Query: 181 FGCGYDQDDGGMQPPPSVAGVLGLSSSKATIVSQLSDLGHVSNVIGHCLAGLGGGFLFFG 240
           FGCGYDQDDGG QPPPSVAGVLGLS SKATIVSQLSDLGHVSNVIGHCLA LGGGFLFFG
Sbjct: 181 FGCGYDQDDGGTQPPPSVAGVLGLSCSKATIVSQLSDLGHVSNVIGHCLAELGGGFLFFG 240

Query: 241 GGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYG 300
            GLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYG
Sbjct: 241 AGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYG 300

Query: 301 AVEKLLKNDLKGQPLKLAPDDKTLELCWKGPKAFKSVSDVRDFFKPLALNFKNSKNAQFQ 360
           AVEKLLKNDLKGQPLKLAPDDKTLELCWKG KAFKSVSDVRDFFKPLALNFKNSKNAQFQ
Sbjct: 301 AVEKLLKNDLKGQPLKLAPDDKTLELCWKGRKAFKSVSDVRDFFKPLALNFKNSKNAQFQ 360

Query: 361 IPPEAYLIIS 370
           IPPEAYLIIS
Sbjct: 361 IPPEAYLIIS 370

BLAST of Csor.00g227030 vs. ExPASy TrEMBL
Match: A0A6J1FCF0 (aspartic proteinase Asp1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442825 PE=3 SV=1)

HSP 1 Score: 861 bits (2224), Expect = 1.72e-315
Identity = 421/432 (97.45%), Postives = 426/432 (98.61%), Query Frame = 0

Query: 1   MEDKKSSLVSGSLEIRMPVLLFVSILFAIFAVSLSDKFLTADWEQLRSVGFGPSILFPVK 60
           MEDKKSSLVSGSLEIRMPVLLFVSILFAIFAVSLSD+FLTADWEQLRSVGFG SILFPVK
Sbjct: 1   MEDKKSSLVSGSLEIRMPVLLFVSILFAIFAVSLSDQFLTADWEQLRSVGFGSSILFPVK 60

Query: 61  GNVYPLGHFTVSVKIGNPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVS 120
           GNVYPLGHFTVSV IGNPPKAFELDIDTGSDLTWVQCDVQC GCTLPRDMLYKPHNNAVS
Sbjct: 61  GNVYPLGHFTVSVTIGNPPKAFELDIDTGSDLTWVQCDVQCTGCTLPRDMLYKPHNNAVS 120

Query: 121 REDPLCAALSSPGNAPCKKSNDQCDYEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG 180
           REDPLCAALSSPGNAPCKKSNDQCDY+VEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG
Sbjct: 121 REDPLCAALSSPGNAPCKKSNDQCDYDVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG 180

Query: 181 FGCGYDQDDGGMQPPPSVAGVLGLSSSKATIVSQLSDLGHVSNVIGHCLAGLGGGFLFFG 240
           FGCGYDQD GGMQPPP VAGVLGLSSSKATIVSQL+DLGHVSNVIGHCLAGLGGGFLFFG
Sbjct: 181 FGCGYDQDYGGMQPPPLVAGVLGLSSSKATIVSQLNDLGHVSNVIGHCLAGLGGGFLFFG 240

Query: 241 GGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYG 300
           GGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGI+GLTLTFDSGSSYTYFNSQVYG
Sbjct: 241 GGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIKGLTLTFDSGSSYTYFNSQVYG 300

Query: 301 AVEKLLKNDLKGQPLKLAPDDKTLELCWKGPKAFKSVSDVRDFFKPLALNFKNSKNAQFQ 360
           AVEKLLKNDLKGQPLKLAPDDKTLELCWKG KAFKSVSDVRDFFKPLALNFKNSKNAQFQ
Sbjct: 301 AVEKLLKNDLKGQPLKLAPDDKTLELCWKGRKAFKSVSDVRDFFKPLALNFKNSKNAQFQ 360

Query: 361 IPPEAYLIISEFGNVCLGILDGTKEGLGNVNLIGDISMLNKIVVYDNQRRQIGWAPQNCN 420
           IPPEAYLIISEFGNVCLGILDGTKEGLGNVNLIGDISMLNKIVVYDNQRRQIGWAPQNCN
Sbjct: 361 IPPEAYLIISEFGNVCLGILDGTKEGLGNVNLIGDISMLNKIVVYDNQRRQIGWAPQNCN 420

Query: 421 PPPRNEAAYGDQ 432
           PPPRNEA+YGDQ
Sbjct: 421 PPPRNEASYGDQ 432

BLAST of Csor.00g227030 vs. ExPASy TrEMBL
Match: A0A6J1IHP1 (aspartic proteinase Asp1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111477022 PE=4 SV=1)

HSP 1 Score: 859 bits (2219), Expect = 1.12e-314
Identity = 422/432 (97.69%), Postives = 423/432 (97.92%), Query Frame = 0

Query: 1   MEDKKSSLVSGSLEIRMPVLLFVSILFAIFAVSLSDKFLTADWEQLRSVGFGPSILFPVK 60
           MEDKKSS VSGSLEIRMPVLLFVSILFAIFAVSLSDKFLTADWEQLRSVGFG SILFPVK
Sbjct: 1   MEDKKSSFVSGSLEIRMPVLLFVSILFAIFAVSLSDKFLTADWEQLRSVGFGSSILFPVK 60

Query: 61  GNVYPLGHFTVSVKIGNPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVS 120
           GNVYPLGHFTVSVKIG PPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVS
Sbjct: 61  GNVYPLGHFTVSVKIGKPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVS 120

Query: 121 REDPLCAALSSPGNAPCKKSNDQCDYEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG 180
           REDPLCAALSSPGNAPCKKSNDQC YEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG
Sbjct: 121 REDPLCAALSSPGNAPCKKSNDQCVYEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG 180

Query: 181 FGCGYDQDDGGMQPPPSVAGVLGLSSSKATIVSQLSDLGHVSNVIGHCLAGLGGGFLFFG 240
           FGCGYDQDDGG QPPPSVAGVLGLS SKATIVSQLSDLGHVSNVIGHCLA LGGGFLFFG
Sbjct: 181 FGCGYDQDDGGTQPPPSVAGVLGLSCSKATIVSQLSDLGHVSNVIGHCLAELGGGFLFFG 240

Query: 241 GGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYG 300
            GLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYG
Sbjct: 241 AGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYG 300

Query: 301 AVEKLLKNDLKGQPLKLAPDDKTLELCWKGPKAFKSVSDVRDFFKPLALNFKNSKNAQFQ 360
           AVEKLLKNDLKGQPLKLAPDDKTLELCWKG KAFKSVSDVRDFFKPLALNFKNSKNAQFQ
Sbjct: 301 AVEKLLKNDLKGQPLKLAPDDKTLELCWKGRKAFKSVSDVRDFFKPLALNFKNSKNAQFQ 360

Query: 361 IPPEAYLIISEFGNVCLGILDGTKEGLGNVNLIGDISMLNKIVVYDNQRRQIGWAPQNCN 420
           IPPEAYLIISEFGNVCLGILDGTKEGLGNVNLIGDISMLNKIVVYDNQRRQIGWAPQNCN
Sbjct: 361 IPPEAYLIISEFGNVCLGILDGTKEGLGNVNLIGDISMLNKIVVYDNQRRQIGWAPQNCN 420

Query: 421 PPPRNEAAYGDQ 432
           PPPRNEA+YGDQ
Sbjct: 421 PPPRNEASYGDQ 432

BLAST of Csor.00g227030 vs. ExPASy TrEMBL
Match: A0A6J1F7L0 (aspartic proteinase Asp1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442825 PE=3 SV=1)

HSP 1 Score: 730 bits (1884), Expect = 1.41e-259
Identity = 360/370 (97.30%), Postives = 364/370 (98.38%), Query Frame = 0

Query: 1   MEDKKSSLVSGSLEIRMPVLLFVSILFAIFAVSLSDKFLTADWEQLRSVGFGPSILFPVK 60
           MEDKKSSLVSGSLEIRMPVLLFVSILFAIFAVSLSD+FLTADWEQLRSVGFG SILFPVK
Sbjct: 1   MEDKKSSLVSGSLEIRMPVLLFVSILFAIFAVSLSDQFLTADWEQLRSVGFGSSILFPVK 60

Query: 61  GNVYPLGHFTVSVKIGNPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVS 120
           GNVYPLGHFTVSV IGNPPKAFELDIDTGSDLTWVQCDVQC GCTLPRDMLYKPHNNAVS
Sbjct: 61  GNVYPLGHFTVSVTIGNPPKAFELDIDTGSDLTWVQCDVQCTGCTLPRDMLYKPHNNAVS 120

Query: 121 REDPLCAALSSPGNAPCKKSNDQCDYEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG 180
           REDPLCAALSSPGNAPCKKSNDQCDY+VEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG
Sbjct: 121 REDPLCAALSSPGNAPCKKSNDQCDYDVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG 180

Query: 181 FGCGYDQDDGGMQPPPSVAGVLGLSSSKATIVSQLSDLGHVSNVIGHCLAGLGGGFLFFG 240
           FGCGYDQD GGMQPPP VAGVLGLSSSKATIVSQL+DLGHVSNVIGHCLAGLGGGFLFFG
Sbjct: 181 FGCGYDQDYGGMQPPPLVAGVLGLSSSKATIVSQLNDLGHVSNVIGHCLAGLGGGFLFFG 240

Query: 241 GGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYG 300
           GGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGI+GLTLTFDSGSSYTYFNSQVYG
Sbjct: 241 GGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIKGLTLTFDSGSSYTYFNSQVYG 300

Query: 301 AVEKLLKNDLKGQPLKLAPDDKTLELCWKGPKAFKSVSDVRDFFKPLALNFKNSKNAQFQ 360
           AVEKLLKNDLKGQPLKLAPDDKTLELCWKG KAFKSVSDVRDFFKPLALNFKNSKNAQFQ
Sbjct: 301 AVEKLLKNDLKGQPLKLAPDDKTLELCWKGRKAFKSVSDVRDFFKPLALNFKNSKNAQFQ 360

Query: 361 IPPEAYLIIS 370
           IPPEAYLIIS
Sbjct: 361 IPPEAYLIIS 370

BLAST of Csor.00g227030 vs. ExPASy TrEMBL
Match: A0A6J1IK95 (aspartic proteinase Asp1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477022 PE=4 SV=1)

HSP 1 Score: 728 bits (1879), Expect = 8.10e-259
Identity = 361/370 (97.57%), Postives = 361/370 (97.57%), Query Frame = 0

Query: 1   MEDKKSSLVSGSLEIRMPVLLFVSILFAIFAVSLSDKFLTADWEQLRSVGFGPSILFPVK 60
           MEDKKSS VSGSLEIRMPVLLFVSILFAIFAVSLSDKFLTADWEQLRSVGFG SILFPVK
Sbjct: 1   MEDKKSSFVSGSLEIRMPVLLFVSILFAIFAVSLSDKFLTADWEQLRSVGFGSSILFPVK 60

Query: 61  GNVYPLGHFTVSVKIGNPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVS 120
           GNVYPLGHFTVSVKIG PPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVS
Sbjct: 61  GNVYPLGHFTVSVKIGKPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVS 120

Query: 121 REDPLCAALSSPGNAPCKKSNDQCDYEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG 180
           REDPLCAALSSPGNAPCKKSNDQC YEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG
Sbjct: 121 REDPLCAALSSPGNAPCKKSNDQCVYEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLG 180

Query: 181 FGCGYDQDDGGMQPPPSVAGVLGLSSSKATIVSQLSDLGHVSNVIGHCLAGLGGGFLFFG 240
           FGCGYDQDDGG QPPPSVAGVLGLS SKATIVSQLSDLGHVSNVIGHCLA LGGGFLFFG
Sbjct: 181 FGCGYDQDDGGTQPPPSVAGVLGLSCSKATIVSQLSDLGHVSNVIGHCLAELGGGFLFFG 240

Query: 241 GGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYG 300
            GLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYG
Sbjct: 241 AGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYG 300

Query: 301 AVEKLLKNDLKGQPLKLAPDDKTLELCWKGPKAFKSVSDVRDFFKPLALNFKNSKNAQFQ 360
           AVEKLLKNDLKGQPLKLAPDDKTLELCWKG KAFKSVSDVRDFFKPLALNFKNSKNAQFQ
Sbjct: 301 AVEKLLKNDLKGQPLKLAPDDKTLELCWKGRKAFKSVSDVRDFFKPLALNFKNSKNAQFQ 360

Query: 361 IPPEAYLIIS 370
           IPPEAYLIIS
Sbjct: 361 IPPEAYLIIS 370

BLAST of Csor.00g227030 vs. ExPASy TrEMBL
Match: A0A0A0L1V4 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G361860 PE=4 SV=1)

HSP 1 Score: 707 bits (1826), Expect = 4.28e-255
Identity = 342/413 (82.81%), Postives = 373/413 (90.31%), Query Frame = 0

Query: 17  MPVLLFVSILFAIFAVSLSDKFLTADWEQLRSVGFGPSILFPVKGNVYPLGHFTVSVKIG 76
           MP LL VSILFA FAVSLSDKFL AD EQ++++ FG S+LFPV+GNVYPLGHFTV + IG
Sbjct: 1   MPTLLLVSILFASFAVSLSDKFLFADSEQVKTLRFGSSVLFPVRGNVYPLGHFTVLLNIG 60

Query: 77  NPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVSREDPLCAALSSPGNAP 136
           NP K FELDIDTGSDLTWVQCDV+CIGCTLPRDMLY+PHNNAVSREDPLCAALSS G   
Sbjct: 61  NPSKVFELDIDTGSDLTWVQCDVECIGCTLPRDMLYRPHNNAVSREDPLCAALSSLGKFI 120

Query: 137 CKKSNDQCDYEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLGFGCGYDQDDGGMQPPP 196
            K  NDQC YEVEYADHGSS+GVLVKDL PMRLTNG+ I+PNLGFGCGYDQ++G +Q PP
Sbjct: 121 FKNPNDQCAYEVEYADHGSSVGVLVKDLVPMRLTNGKRISPNLGFGCGYDQENGDLQQPP 180

Query: 197 SVAGVLGLSSSKATIVSQLSDLGHVSNVIGHCLAGLGGGFLFFGGGLVPSSGMSWTPILR 256
           S+AGVLGLSSSKATIVSQLSDLGHVSNV+GHCL G GGGFLFFGG +VPSSGMSWTPILR
Sbjct: 181 SIAGVLGLSSSKATIVSQLSDLGHVSNVVGHCLTGRGGGFLFFGGDVVPSSGMSWTPILR 240

Query: 257 NSEGKYSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYGAVEKLLKNDLKGQPLK 316
           NSEGKYSSGPAEVYF G+A GI GLTLTFDSGSSYTYFNSQVY A+EKLLKNDLKG PLK
Sbjct: 241 NSEGKYSSGPAEVYFNGRAVGIGGLTLTFDSGSSYTYFNSQVYRAIEKLLKNDLKGNPLK 300

Query: 317 LAPDDKTLELCWKGPKAFKSVSDVRDFFKPLALNFKNSKNAQFQIPPEAYLIISEFGNVC 376
           LA DDKTLELCWKGPK F+SV DVR+FFKPLA++FKNSKN QFQIPPEAYLIISEFGNVC
Sbjct: 301 LASDDKTLELCWKGPKPFESVVDVRNFFKPLAMSFKNSKNVQFQIPPEAYLIISEFGNVC 360

Query: 377 LGILDGTKEGLGNVNLIGDISMLNKIVVYDNQRRQIGWAPQNCNPPPRNEAAY 429
           LGILDG+KEG+GNVN+IGDISMLNKIVVYDN+R +IGWA  NCN  PRNEA++
Sbjct: 361 LGILDGSKEGMGNVNIIGDISMLNKIVVYDNERERIGWASSNCNRSPRNEASW 413

BLAST of Csor.00g227030 vs. TAIR 10
Match: AT1G44130.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 441.0 bits (1133), Expect = 1.1e-123
Identity = 198/372 (53.23%), Postives = 268/372 (72.04%), Query Frame = 0

Query: 54  SILFPVKGNVYPLGHFTVSVKIGNPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYK 113
           S++FP+ GNV+PLG+++V ++IG+PPKAF+ DIDTGSDLTWVQCD  C GCTLP ++ YK
Sbjct: 34  SVVFPLSGNVFPLGYYSVLMQIGSPPKAFQFDIDTGSDLTWVQCDAPCSGCTLPPNLQYK 93

Query: 114 PHNNAVSREDPLCAALSSPGNAPCKKSNDQCDYEVEYADHGSSIGVLVKDLAPMRLTNGQ 173
           P  N +   +P+C AL  P    C    +QCDYEV+YAD GSS+G LV D  P++L NG 
Sbjct: 94  PKGNIIPCSNPICTALHWPNKPHCPNPQEQCDYEVKYADQGSSMGALVTDQFPLKLVNGS 153

Query: 174 LINPNLGFGCGYDQDDGGMQPPPSVAGVLGLSSSKATIVSQLSDLGHVSNVIGHCLAGLG 233
            + P + FGCGYDQ      PPP+ AGVLGL   K  +++QL   G   NV+GHCL+  G
Sbjct: 154 FMQPPVAFGCGYDQSYPSAHPPPATAGVLGLGRGKIGLLTQLVSAGLTRNVVGHCLSSKG 213

Query: 234 GGFLFFGGGLVPSSGMSWTPILRNSEGKYSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTY 293
           GGFLFFG  LVPS G++WTP+L + +  Y++GPA++ F GK  G++GL L FD+GSSYTY
Sbjct: 214 GGFLFFGDNLVPSIGVAWTPLL-SQDNHYTTGPADLLFNGKPTGLKGLKLIFDTGSSYTY 273

Query: 294 FNSQVYGAVEKLLKNDLKGQPLKLAPDDKTLELCWKGPKAFKSVSDVRDFFKPLALNFKN 353
           FNS+ Y  +  L+ NDLK  PLK+A +DKTL +CWKG K FKSV +V++FFK + +NF N
Sbjct: 274 FNSKAYQTIINLIGNDLKVSPLKVAKEDKTLPICWKGAKPFKSVLEVKNFFKTITINFTN 333

Query: 354 S-KNAQFQIPPEAYLIISEFGNVCLGILDGTKEGLGNVNLIGDISMLNKIVVYDNQRRQI 413
             +N Q  + PE YLI+S+ GNVCLG+L+G++ GL N N+IGDISM   +++YDN+++Q+
Sbjct: 334 GRRNTQLYLAPELYLIVSKTGNVCLGLLNGSEVGLQNSNVIGDISMQGLMMIYDNEKQQL 393

Query: 414 GWAPQNCNPPPR 425
           GW   +CN  P+
Sbjct: 394 GWVSSDCNKLPK 404

BLAST of Csor.00g227030 vs. TAIR 10
Match: AT1G77480.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 432.6 bits (1111), Expect = 3.8e-121
Identity = 208/412 (50.49%), Postives = 277/412 (67.23%), Query Frame = 0

Query: 20  LLFVSILFAIFAVSLSDKFLTADWEQLRSVGFGPSILFPVKGNVYPLGHFTVSVKIGNPP 79
           + +V IL A F  S + K  +A   +L++     +++FPV GNVYPLG++ V + IGNPP
Sbjct: 19  VFYVFILCARFQTSEATKDSSAQ-VKLQNRRLSSTVVFPVSGNVYPLGYYYVLLNIGNPP 78

Query: 80  KAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVSREDPLCAALSSPGNAPCKK 139
           K F+LDIDTGSDLTWVQCD  C GCT PR   YKP++N +     LC+ L  P + PC  
Sbjct: 79  KLFDLDIDTGSDLTWVQCDAPCNGCTKPRAKQYKPNHNTLPCSHILCSGLDLPQDRPCAD 138

Query: 140 SNDQCDYEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLGFGCGYDQDDGGMQPPPSVA 199
             DQCDYE+ Y+DH SSIG LV D  P++L NG ++N  L FGCGYDQ + G  PPP  A
Sbjct: 139 PEDQCDYEIGYSDHASSIGALVTDEVPLKLANGSIMNLRLTFGCGYDQQNPGPHPPPPTA 198

Query: 200 GVLGLSSSKATIVSQLSDLGHVSNVIGHCLAGLGGGFLFFGGGLVPSSGMSWTPILRNSE 259
           G+LGL   K  + +QL  LG   NVI HCL+  G GFL  G  LVPSSG++WT +  NS 
Sbjct: 199 GILGLGRGKVGLSTQLKSLGITKNVIVHCLSHTGKGFLSIGDELVPSSGVTWTSLATNSP 258

Query: 260 GK-YSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYGAVEKLLKNDLKGQPLKLA 319
            K Y +GPAE+ F  K  G++G+ + FDSGSSYTYFN++ Y A+  L++ DL G+PL   
Sbjct: 259 SKNYMAGPAELLFNDKTTGVKGINVVFDSGSSYTYFNAEAYQAILDLIRKDLNGKPLTDT 318

Query: 320 PDDKTLELCWKGPKAFKSVSDVRDFFKPLALNFKNSKNAQ-FQIPPEAYLIISEFGNVCL 379
            DDK+L +CWKG K  KS+ +V+ +FK + L F N KN Q FQ+PPE+YLII+E G VCL
Sbjct: 319 KDDKSLPVCWKGKKPLKSLDEVKKYFKTITLRFGNQKNGQLFQVPPESYLIITEKGRVCL 378

Query: 380 GILDGTKEGLGNVNLIGDISMLNKIVVYDNQRRQIGWAPQNCNPPPRNEAAY 430
           GIL+GT+ GL   N+IGDIS    +V+YDN++++IGW   +C+  P++E  +
Sbjct: 379 GILNGTEIGLEGYNIIGDISFQGIMVIYDNEKQRIGWISSDCDKLPKSEPLF 429

BLAST of Csor.00g227030 vs. TAIR 10
Match: AT1G77480.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 431.0 bits (1107), Expect = 1.1e-120
Identity = 209/413 (50.61%), Postives = 275/413 (66.59%), Query Frame = 0

Query: 20  LLFVSILFAIFAVSLSDKFLTADWEQLRSVGFGPSILFPVKGNVYPLGHFTVSVKIGNPP 79
           + +V IL A F  S + K  +A   +L++     +++FPV GNVYPLG++ V + IGNPP
Sbjct: 19  VFYVFILCARFQTSEATKDSSAQ-VKLQNRRLSSTVVFPVSGNVYPLGYYYVLLNIGNPP 78

Query: 80  KAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVSREDPLCAALSSPGNAPCKK 139
           K F+LDIDTGSDLTWVQCD  C GCT PR   YKP++N +     LC+ L  P + PC  
Sbjct: 79  KLFDLDIDTGSDLTWVQCDAPCNGCTKPRAKQYKPNHNTLPCSHILCSGLDLPQDRPCAD 138

Query: 140 SNDQCDYEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLGFGCGYDQDDGGMQPPPSVA 199
             DQCDYE+ Y+DH SSIG LV D  P++L NG ++N  L FGCGYDQ + G  PPP  A
Sbjct: 139 PEDQCDYEIGYSDHASSIGALVTDEVPLKLANGSIMNLRLTFGCGYDQQNPGPHPPPPTA 198

Query: 200 GVLGLSSSKATIVSQLSDLGHVSNVIGHCLAGLGGGFLFFGGGLVPSSGMSWTPILRNSE 259
           G+LGL   K  + +QL  LG   NVI HCL+  G GFL  G  LVPSSG++WT +  NS 
Sbjct: 199 GILGLGRGKVGLSTQLKSLGITKNVIVHCLSHTGKGFLSIGDELVPSSGVTWTSLATNSP 258

Query: 260 GK-YSSGPAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYGAVEKLLKNDLKGQPLKLA 319
            K Y +GPAE+ F  K  G++G+ + FDSGSSYTYFN++ Y A+  L++ DL G+PL   
Sbjct: 259 SKNYMAGPAELLFNDKTTGVKGINVVFDSGSSYTYFNAEAYQAILDLIRKDLNGKPLTDT 318

Query: 320 PDDKTLELCWKGPKAFKSVSDVRDFFKPLALNFKNSKNAQ-FQIPPEAYLIISEFGNVCL 379
            DDK+L +CWKG K  KS+ +V+ +FK + L F N KN Q FQ+PPE+YLII+E G VCL
Sbjct: 319 KDDKSLPVCWKGKKPLKSLDEVKKYFKTITLRFGNQKNGQLFQVPPESYLIITEKGRVCL 378

Query: 380 GILDGTKEGLGNVNLIGDISMLNKIVVYDNQRRQIGWAPQNCNPPPRNEAAYG 431
           GIL+GT+ GL   N+IGDIS    +V+YDN++++IGW   +C+  P     YG
Sbjct: 379 GILNGTEIGLEGYNIIGDISFQGIMVIYDNEKQRIGWISSDCDKLPNVNHDYG 430

BLAST of Csor.00g227030 vs. TAIR 10
Match: AT4G33490.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 409.8 bits (1052), Expect = 2.6e-114
Identity = 207/409 (50.61%), Postives = 273/409 (66.75%), Query Frame = 0

Query: 23  VSILFAIFAVSLSDKFLTA-DWEQLRSVGFG-------PSILFPVKGNVYPLGHFTVSVK 82
           V  +  +  +SL   F +A D+   ++ GF         S++FPV GNVYPLG++ V++ 
Sbjct: 6   VRFMIVLMVMSLVLGFSSAVDFRWRKTAGFSDRFTRAVSSVVFPVHGNVYPLGYYNVTIN 65

Query: 83  IGNPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVSREDPLCAALSSPGN 142
           IG PP+ + LD+DTGSDLTW+QCD  C+ C      LY+P ++ +   DPLC AL    N
Sbjct: 66  IGQPPRPYYLDLDTGSDLTWLQCDAPCVRCLEAPHPLYQPSSDLIPCNDPLCKALHLNSN 125

Query: 143 APCKKSNDQCDYEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLGFGCGYDQDDGGMQP 202
             C ++ +QCDYEVEYAD GSS+GVLV+D+  M  T G  + P L  GCGYDQ  G    
Sbjct: 126 QRC-ETPEQCDYEVEYADGGSSLGVLVRDVFSMNYTQGLRLTPRLALGCGYDQIPGASSH 185

Query: 203 PPSVAGVLGLSSSKATIVSQLSDLGHVSNVIGHCLAGLGGGFLFFGGGLVPSSGMSWTPI 262
            P + GVLGL   K +I+SQL   G+V NVIGHCL+ LGGG LFFG  L  SS +SWTP+
Sbjct: 186 HP-LDGVLGLGRGKVSILSQLHSQGYVKNVIGHCLSSLGGGILFFGDDLYDSSRVSWTPM 245

Query: 263 LRNSEGKYSSG-PAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYGAVEKLLKNDLKGQ 322
            R     YS     E+ FGG+  G++ L   FDSGSSYTYFNS+ Y AV  LLK +L G+
Sbjct: 246 SREYSKHYSPAMGGELLFGGRTTGLKNLLTVFDSGSSYTYFNSKAYQAVTYLLKRELSGK 305

Query: 323 PLKLAPDDKTLELCWKGPKAFKSVSDVRDFFKPLALNFKNSKNAQ--FQIPPEAYLIISE 382
           PLK A DD TL LCW+G + F S+ +V+ +FKPLAL+FK    ++  F+IPPEAYLIIS 
Sbjct: 306 PLKEARDDHTLPLCWQGRRPFMSIEEVKKYFKPLALSFKTGWRSKTLFEIPPEAYLIISM 365

Query: 383 FGNVCLGILDGTKEGLGNVNLIGDISMLNKIVVYDNQRRQIGWAPQNCN 421
            GNVCLGIL+GT+ GL N+NLIGDISM +++++YDN+++ IGW P +C+
Sbjct: 366 KGNVCLGILNGTEIGLQNLNLIGDISMQDQMIIYDNEKQSIGWMPVDCD 412

BLAST of Csor.00g227030 vs. TAIR 10
Match: AT4G33490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 375.9 bits (964), Expect = 4.2e-104
Identity = 195/383 (50.91%), Postives = 251/383 (65.54%), Query Frame = 0

Query: 23  VSILFAIFAVSLSDKFLTA-DWEQLRSVGFG-------PSILFPVKGNVYPLGHFTVSVK 82
           V  +  +  +SL   F +A D+   ++ GF         S++FPV GNVYPLG++ V++ 
Sbjct: 3   VRFMIVLMVMSLVLGFSSAVDFRWRKTAGFSDRFTRAVSSVVFPVHGNVYPLGYYNVTIN 62

Query: 83  IGNPPKAFELDIDTGSDLTWVQCDVQCIGCTLPRDMLYKPHNNAVSREDPLCAALSSPGN 142
           IG PP+ + LD+DTGSDLTW+QCD  C+ C      LY+P ++ +   DPLC AL    N
Sbjct: 63  IGQPPRPYYLDLDTGSDLTWLQCDAPCVRCLEAPHPLYQPSSDLIPCNDPLCKALHLNSN 122

Query: 143 APCKKSNDQCDYEVEYADHGSSIGVLVKDLAPMRLTNGQLINPNLGFGCGYDQDDGGMQP 202
             C ++ +QCDYEVEYAD GSS+GVLV+D+  M  T G  + P L  GCGYDQ  G    
Sbjct: 123 QRC-ETPEQCDYEVEYADGGSSLGVLVRDVFSMNYTQGLRLTPRLALGCGYDQIPGASSH 182

Query: 203 PPSVAGVLGLSSSKATIVSQLSDLGHVSNVIGHCLAGLGGGFLFFGGGLVPSSGMSWTPI 262
            P + GVLGL   K +I+SQL   G+V NVIGHCL+ LGGG LFFG  L  SS +SWTP+
Sbjct: 183 HP-LDGVLGLGRGKVSILSQLHSQGYVKNVIGHCLSSLGGGILFFGDDLYDSSRVSWTPM 242

Query: 263 LRNSEGKYSSG-PAEVYFGGKAGGIRGLTLTFDSGSSYTYFNSQVYGAVEKLLKNDLKGQ 322
            R     YS     E+ FGG+  G++ L   FDSGSSYTYFNS+ Y AV  LLK +L G+
Sbjct: 243 SREYSKHYSPAMGGELLFGGRTTGLKNLLTVFDSGSSYTYFNSKAYQAVTYLLKRELSGK 302

Query: 323 PLKLAPDDKTLELCWKGPKAFKSVSDVRDFFKPLALNFKNSKNAQ--FQIPPEAYLIISE 382
           PLK A DD TL LCW+G + F S+ +V+ +FKPLAL+FK    ++  F+IPPEAYLIIS 
Sbjct: 303 PLKEARDDHTLPLCWQGRRPFMSIEEVKKYFKPLALSFKTGWRSKTLFEIPPEAYLIISM 362

Query: 383 FGNVCLGILDGTKEGLGNVNLIG 395
            GNVCLGIL+GT+ GL N+NLIG
Sbjct: 363 KGNVCLGILNGTEIGLQNLNLIG 383

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0IU521.7e-8142.64Aspartic proteinase Asp1 OS=Oryza sativa subsp. japonica OX=39947 GN=ASP1 PE=2 S... [more]
A2ZC679.6e-7741.34Aspartic proteinase Asp1 OS=Oryza sativa subsp. indica OX=39946 GN=ASP1 PE=2 SV=... [more]
Q9M9A81.3e-7641.30Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1[more]
Q9S9K42.4e-3528.54Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2[more]
Q4V3D24.2e-3228.72Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6591879.10.0100.00Aspartic proteinase Asp1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022936138.13.56e-31597.45aspartic proteinase Asp1-like isoform X2 [Cucurbita moschata][more]
XP_022976721.12.31e-31497.69aspartic proteinase Asp1-like isoform X2 [Cucurbita maxima][more]
XP_022936137.12.91e-25997.30aspartic proteinase Asp1-like isoform X1 [Cucurbita moschata][more]
XP_022976720.11.67e-25897.57aspartic proteinase Asp1-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1FCF01.72e-31597.45aspartic proteinase Asp1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1IHP11.12e-31497.69aspartic proteinase Asp1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11147... [more]
A0A6J1F7L01.41e-25997.30aspartic proteinase Asp1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1IK958.10e-25997.57aspartic proteinase Asp1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11147... [more]
A0A0A0L1V44.28e-25582.81Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G36186... [more]
Match NameE-valueIdentityDescription
AT1G44130.11.1e-12353.23Eukaryotic aspartyl protease family protein [more]
AT1G77480.23.8e-12150.49Eukaryotic aspartyl protease family protein [more]
AT1G77480.11.1e-12050.61Eukaryotic aspartyl protease family protein [more]
AT4G33490.22.6e-11450.61Eukaryotic aspartyl protease family protein [more]
AT4G33490.14.2e-10450.91Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 391..406
score: 27.39
coord: 75..95
score: 46.1
coord: 283..294
score: 27.34
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 21..422
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 244..421
e-value: 8.5E-31
score: 108.7
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 52..240
e-value: 3.7E-39
score: 136.6
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 61..419
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 284..415
e-value: 8.9E-16
score: 58.1
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 69..241
e-value: 7.5E-40
score: 137.0
NoneNo IPR availablePANTHERPTHR13683:SF227EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEINcoord: 21..422
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 84..95
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 69..415
score: 33.938034

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g227030.m01Csor.00g227030.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity