Csor.00g225840 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g225840
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionpalmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like
LocationCsor_Chr10: 5645862 .. 5647993 (-)
RNA-Seq ExpressionCsor.00g225840
SyntenyCsor.00g225840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACACAGCAAAGCCTCGAAGATCATCGGGGAAGGAAGAATGGGCTAAGATAGACAAACAAAAGGCCATTTTTATTTTTCTTATGCATGTGCTTTGTATTTTTGCACCATTTCAAGTCAATTCGAGAGCGCTTCGGATTGCTTTGGCATTACATTTTATCACCGGTTTCTTCGGCATAACTCTCTCATATCATAGAAATCTTTCCCACCGGAGTTTCAAGCTTCCGAAATGGCTCGAGTATTTGTTTGCTTTATGTGGAGCTCACACACTCCTGGTAAATTAAATGATAGATTCTTAAGCATGGTAAAATATTTACTATGTTTTGAAGTTTTTCGTTGTTTTTTATAGGGTGATCCGATCAATTGGGTCGGTATGCACAGGTATCACCACCAGGTAGCCGACACCGAAAAAGATCCCCAACGTACTCCTCGAAGGTTCTTGTGGCTTTCTAATTACTTTGCTTCAACTAAAAGAGTTGGTCCAAGGTACTTATTTATTCTTGAAGGGATAGAAAAGAACATATTTTTAATGATTAGGGAGTTTGTGAAACGAGATAATGTTGGCGACTTGGAGAATCAAAGCTTCTACAAGTTTATTCACAAAACATATCTTCTTCATCCAATTGGTCTTGCAATCTTGTTGTATGTTGTTGGAGGAATGCCTTTTCTTGTATGGGGAATGGTACGTATAGAAACGCTCTCATTTCATCGTGAAATTTCGAAATCTTATATTTTTATCAATGAATTTCAAGTTCAGCTTTTATAGTCCAATAGGCACTTGAATTTTAAAAAGGGTCAAATAGATATCTAAGTATTTAACTTTATGTCTAATAAACTTATAAACTTTTGAAACACTTATTAAGTTCGTACACTTTAAAGTTTTTGTCTACAAGATCGGTGAGATTTCACATCGATTGGGAAGGAGAACAAAACATTCTTTGTAAGGGTATGAAAACCTCTCTCTAGCAGACGCGTTTTAAAAATCTTGAGGGGAAACACGAAGGAGAAAACCCAAAGAGGACAATATCTATTAGCAGTGGACTTGGGCTGTTACAAATGGTATCGGGGTGGATTGTGAGATCCCACATCCTAGCATACGTGTTTTAAAAACCCGGTTGTTACAAATGGTATTAGGGTTGATTGTGAGATCCTACACCCTAGTAGACATGCTTTAAAAACTTTGGGCGGAAGCTCGAAAGGGAAAGTCTAAAGAGGACAATATGTGCTAGTGGTAGGCCTGGGCTGTTACAAGTTCTTTATTGTGAGACCCCACACCCTAGCAAACACATTTTAAAAATCTTGGGGGAAGCTGAAAAGACAAAATCCAAAGATAACAATATCTGCTGCTGATGGTGGGCTTGGGCTGTTACAAGTTCTTTATTGTGAGATCCTATAGTCTATCAGACATGTTTTAAAAACCTTGGAGTAAGCTCGAAAGGGAAAACCCAAAAAAGACAATATCTGTTGCCGATGGGCCTGGGCTGTTACAAGATCATTATTTGTATTTTAATAAAGCACATTTAATTTTTTTTATCAAACCGATCTCTAAACTTTATATTTTGTATCTAACATATGTGTGAATTTAAATAATAAATATCAAATATGTTAAGAACTTATAAGATACAAAATTAAACACATAATTTTATTTGATCATGTAGGGTGTGCGAACCGTACTAGTGTTACATCAATCATTAATGGTAAACACAATATGCCATAGATTCGGAAAACGACAATGGAAGACAAATGATTCGTCCAGAAACAACGGGTAAGTAGACATTATTGAATTAGTAAGCTTTGAAATATTTTAACCTTTCTTTTGTAGTAGAATTTACCAAACACGAACTCAACATGTTTGAAAACAAAGCATTAGGGTTTTGGTATGTACAATAATTGATTCTTGTTGTGTTGTAGGTTGATGAGTTTGATTAGCTTTGGAGAAGGTTGGCATAATAATCACCATGCCTTTCCTTACTCAGCTAGGCATGGATTTGAATGGTGGCAGGTCGACACTAGTTGGTACGCTATTAGGTTTCTTCAAGCCATTGGTGTTGCCACTGATGTCAAATTGCCTCCCCAGCAAAGCCGCAGAACCAAAAGCTTGATCATGGACAACCAGGTGCTGCAGCACTAA

mRNA sequence

ATGGACACAGCAAAGCCTCGAAGATCATCGGGGAAGGAAGAATGGGCTAAGATAGACAAACAAAAGGCCATTTTTATTTTTCTTATGCATGTGCTTTGTATTTTTGCACCATTTCAAGTCAATTCGAGAGCGCTTCGGATTGCTTTGGCATTACATTTTATCACCGGTTTCTTCGGCATAACTCTCTCATATCATAGAAATCTTTCCCACCGGAGTTTCAAGCTTCCGAAATGGCTCGAGTATTTGTTTGCTTTATGTGGAGCTCACACACTCCTGGGTGATCCGATCAATTGGGTCGGTATGCACAGGTATCACCACCAGGTAGCCGACACCGAAAAAGATCCCCAACGTACTCCTCGAAGGGAGTTTGTGAAACGAGATAATGTTGGCGACTTGGAGAATCAAAGCTTCTACAAGTTTATTCACAAAACATATCTTCTTCATCCAATTGGTCTTGCAATCTTGTTGTATGTTGTTGGAGGAATGCCTTTTCTTGTATGGGGAATGGGTGTGCGAACCGTACTAGTGTTACATCAATCATTAATGGTAAACACAATATGCCATAGATTCGGAAAACGACAATGGAAGACAAATGATTCGTCCAGAAACAACGGGTTGATGAGTTTGATTAGCTTTGGAGAAGGTTGGCATAATAATCACCATGCCTTTCCTTACTCAGCTAGGCATGGATTTGAATGGTGGCAGGTCGACACTAGTTGGTACGCTATTAGGTTTCTTCAAGCCATTGGTGTTGCCACTGATGTCAAATTGCCTCCCCAGCAAAGCCGCAGAACCAAAAGCTTGATCATGGACAACCAGGTGCTGCAGCACTAA

Coding sequence (CDS)

ATGGACACAGCAAAGCCTCGAAGATCATCGGGGAAGGAAGAATGGGCTAAGATAGACAAACAAAAGGCCATTTTTATTTTTCTTATGCATGTGCTTTGTATTTTTGCACCATTTCAAGTCAATTCGAGAGCGCTTCGGATTGCTTTGGCATTACATTTTATCACCGGTTTCTTCGGCATAACTCTCTCATATCATAGAAATCTTTCCCACCGGAGTTTCAAGCTTCCGAAATGGCTCGAGTATTTGTTTGCTTTATGTGGAGCTCACACACTCCTGGGTGATCCGATCAATTGGGTCGGTATGCACAGGTATCACCACCAGGTAGCCGACACCGAAAAAGATCCCCAACGTACTCCTCGAAGGGAGTTTGTGAAACGAGATAATGTTGGCGACTTGGAGAATCAAAGCTTCTACAAGTTTATTCACAAAACATATCTTCTTCATCCAATTGGTCTTGCAATCTTGTTGTATGTTGTTGGAGGAATGCCTTTTCTTGTATGGGGAATGGGTGTGCGAACCGTACTAGTGTTACATCAATCATTAATGGTAAACACAATATGCCATAGATTCGGAAAACGACAATGGAAGACAAATGATTCGTCCAGAAACAACGGGTTGATGAGTTTGATTAGCTTTGGAGAAGGTTGGCATAATAATCACCATGCCTTTCCTTACTCAGCTAGGCATGGATTTGAATGGTGGCAGGTCGACACTAGTTGGTACGCTATTAGGTTTCTTCAAGCCATTGGTGTTGCCACTGATGTCAAATTGCCTCCCCAGCAAAGCCGCAGAACCAAAAGCTTGATCATGGACAACCAGGTGCTGCAGCACTAA

Protein sequence

MDTAKPRRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRALRIALALHFITGFFGITLSYHRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVGMHRYHHQVADTEKDPQRTPRREFVKRDNVGDLENQSFYKFIHKTYLLHPIGLAILLYVVGGMPFLVWGMGVRTVLVLHQSLMVNTICHRFGKRQWKTNDSSRNNGLMSLISFGEGWHNNHHAFPYSARHGFEWWQVDTSWYAIRFLQAIGVATDVKLPPQQSRRTKSLIMDNQVLQH
Homology
BLAST of Csor.00g225840 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 316.2 bits (809), Expect = 3.6e-85
Identity = 142/277 (51.26%), Postives = 189/277 (68.23%), Query Frame = 0

Query: 12  KEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRALRIALALHFITGFFGITLSYHRNLSHR 71
           + EW  +D      +  MH+L + APFQ N RA+ +A  L+ +TG  GITLS+HRNLSH+
Sbjct: 95  EREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHK 154

Query: 72  SFKLPKWLEYLFALCGAHTLLGDPINWVGMHRYHHQVADTEKDPQ--------------- 131
           +FKLPKWLEYLFA CGA  L G+PI+WV  HRYHHQ  D+++DP                
Sbjct: 155 AFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMF 214

Query: 132 --RTPRREFVKRDNVGDLENQSFYKFIHKTYLLHPIGLAILLYVVGGMPFLVWGMGVRTV 191
              T  +   + +NVGDLE Q FY+F+  TY+LHP+ LA+ LY +GG PF+VWGMGVR V
Sbjct: 215 DTNTITQRCGEPNNVGDLEKQPFYRFLRTTYILHPLALAVALYAMGGFPFIVWGMGVRIV 274

Query: 192 LVLHQSLMVNTICHRFGKRQWKTNDSSRNNGLMSLISFGEGWHNNHHAFPYSARHGFEWW 251
            V H + +VN+ CH +GK+ W T D S+NN  ++ ++FGEGWHNNHHAF +SARHG EWW
Sbjct: 275 WVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAFGEGWHNNHHAFEFSARHGLEWW 334

Query: 252 QVDTSWYAIRFLQAIGVATDVKLPPQQSRRTKSLIMD 272
           Q+D +WY ++FLQAIG+ATDVKLP +  ++  +   D
Sbjct: 335 QLDMTWYVVKFLQAIGLATDVKLPSEAQKQRMAFTSD 371

BLAST of Csor.00g225840 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.0e-76
Identity = 134/286 (46.85%), Postives = 183/286 (63.99%), Query Frame = 0

Query: 2   DTAKPRRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRALRIALALHFITGFFGIT 61
           +  + +R+  + +W ++D  KA     +H LC+ APF     ALR+AL ++ + G  GIT
Sbjct: 21  EMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGG-LGIT 80

Query: 62  LSYHRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVGMHRYHHQVADTEKDPQRTPRR 121
           +SYHRNL+HRSFK+PKWLEY FA CG   + GDPI+WV  HRYHHQ  D+++DP  +P  
Sbjct: 81  VSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDP-HSPNE 140

Query: 122 EF------------------VKRDNVGDLENQSFYKFIHKTYLLHPIGLAILLYVVGGMP 181
            F                   +R NV DL+ Q +YKF+ +T L H +    LLY  GG+ 
Sbjct: 141 GFWFSHLLWLFDTGYLVEKCGRRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLS 200

Query: 182 FLVWGMGVRTVLVLHQSLMVNTICHRFGKRQWKTNDSSRNNGLMSLISFGEGWHNNHHAF 241
           FL WGMG+   +  H + ++N++CH +G R WKTND+SRN   +S+ SFGE WHNNHHAF
Sbjct: 201 FLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRNVWWLSVFSFGESWHNNHHAF 260

Query: 242 PYSARHGFEWWQVDTSWYAIRFLQAIGVATDVKLPPQQSRRTKSLI 270
             SAR G EWWQ+D SWY +RFL+ IG+ATDVKLP +  RR  +++
Sbjct: 261 ESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQRRRMAMV 304

BLAST of Csor.00g225840 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 2.6e-75
Identity = 133/281 (47.33%), Postives = 181/281 (64.41%), Query Frame = 0

Query: 5   KPRRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRALRIALALHFITGFFGITLSY 64
           K ++ +   +W   D    +FI  +HVLC+FAP   + ++  +  AL+ I G FG TLS+
Sbjct: 30  KTKKITWVRKWNSTDVFHILFIGGLHVLCLFAPSTFSWKSFWVCFALYAICGVFGTTLSF 89

Query: 65  HRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVGMHRYHHQVADTEKDPQRTPRREF- 124
           HRNL+HRSFKLPK+LEY FA  G H L GDP+ WV  HRYHH+  DT  DP  +P   F 
Sbjct: 90  HRNLTHRSFKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKYTDTYLDP-HSPIEGFW 149

Query: 125 -----------------VKRDNVGDLENQSFYKFIHKTYLLHPIGLAILLYVVGGMPFLV 184
                             + +N GDL  QS+Y+F+ +T++ H    A LLY+ GG PF+V
Sbjct: 150 FCHIFWLFDSKYIIEKCGRYENAGDLMKQSYYRFLERTFVYHVYLQAALLYLFGGFPFIV 209

Query: 185 WGMGVRTVLVLHQSLMVNTICHRFGKRQWKTNDSSRNNGLMSLISFGEGWHNNHHAFPYS 244
           WGM VRT+L  H S +VN++CHR+G R W T D S NN  +++++ GEGWHNNHHAF YS
Sbjct: 210 WGMAVRTILGFHLSWLVNSVCHRWGNRPWNTGDLSTNNWFIAMLTSGEGWHNNHHAFEYS 269

Query: 245 ARHGFEWWQVDTSWYAIRFLQAIGVATDVKLPPQQSRRTKS 268
           ARHG EWWQ+DT+WY I+ L+ +G+ATD+K+P +  +R  S
Sbjct: 270 ARHGIEWWQIDTTWYIIKLLEYLGLATDIKVPSEIHKRKMS 309

BLAST of Csor.00g225840 vs. ExPASy Swiss-Prot
Match: P0DOW3 (Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 2.2e-74
Identity = 134/289 (46.37%), Postives = 181/289 (62.63%), Query Frame = 0

Query: 5   KPRRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRALRIALALHFITGFFGITLSY 64
           K ++ +    W   D    I +  +HVLC+ APF  +  A  ++L L+ + G FG TLSY
Sbjct: 27  KTKKITWVRVWNSTDIFHIILVGGLHVLCLSAPFTFSWSAFWLSLTLYAVCGVFGTTLSY 86

Query: 65  HRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVGMHRYHHQVADTEKDP--------- 124
           HRNL+HRSFKLPK+LEY FA  G H L GDP+ WV  HRYHH+  DT  DP         
Sbjct: 87  HRNLTHRSFKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKFTDTYLDPHSPIEGFWF 146

Query: 125 --------QRTPRREFVKRDNVGDLENQSFYKFIHKTYLLHPIGLAILLYVVGGMPFLVW 184
                    +    E  + +N GDL  QS+Y+F+ +T++ H    A LLY+ GG PF+VW
Sbjct: 147 SHIFWLFDSKYILEECGRYENAGDLLKQSYYRFLERTFVFHVYLQAALLYMFGGFPFIVW 206

Query: 185 GMGVRTVLVLHQSLMVNTICHRFGKRQWKTNDSSRNNGLMSLISFGEGWHNNHHAFPYSA 244
           GM VRTV   H S +VN++CHR+G + W T D S NN  +++++ GEGWHNNHHAF YSA
Sbjct: 207 GMAVRTVWGFHASWLVNSVCHRYGHQAWDTGDLSTNNWFIAMLTSGEGWHNNHHAFEYSA 266

Query: 245 RHGFEWWQVDTSWYAIRFLQAIGVATDVKLPPQQSRRT---KSLIMDNQ 274
           RHG EWWQ+DT+WY I+ L+ +G+ATDVK+P +  +R    K+ + D Q
Sbjct: 267 RHGIEWWQIDTTWYVIKLLEYLGLATDVKVPSEVHKRKMSFKNCVQDKQ 315

BLAST of Csor.00g225840 vs. ExPASy Swiss-Prot
Match: Q9SID2 (Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=2)

HSP 1 Score: 275.0 bits (702), Expect = 9.2e-73
Identity = 131/273 (47.99%), Postives = 173/273 (63.37%), Query Frame = 0

Query: 15  WAKIDKQKAIFIFLMHVLCIFAPFQVNSRALRIALALHFITGFFGITLSYHRNLSHRSFK 74
           W ++D  K    F +H L + APF     AL +   L +  G  GIT+SYHRNL+HRSFK
Sbjct: 36  WRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTF-LFYTIGGLGITVSYHRNLAHRSFK 95

Query: 75  LPKWLEYLFALCGAHTLLGDPINWVGMHRYHHQVADTEKDPQRTPRREF----------- 134
           +PKWLEYL A C    + GDPI+WV  HRYHHQ  D+E+DP  +P+  F           
Sbjct: 96  VPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDP-HSPKEGFWFSHLLWIYDS 155

Query: 135 -------VKRDNVGDLENQSFYKFIHKTYLLHPIGLAILLYVVGGMPFLVWGMGVRTVLV 194
                   +R NV DL+ Q FY+F+ KT L H +GL   L+ +GGM F+ WGMGV   L 
Sbjct: 156 AYLVSKCGRRANVEDLKRQWFYRFLQKTVLFHILGLGFFLFYLGGMSFVTWGMGVGAALE 215

Query: 195 LHQSLMVNTICHRFGKRQWKTNDSSRNNGLMSLISFGEGWHNNHHAFPYSARHGFEWWQV 254
           +H + ++N++CH +G R WKTND+SRN   +S+ SFGE WHNNHHAF  SAR G EWWQ+
Sbjct: 216 VHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQI 275

Query: 255 DTSWYAIRFLQAIGVATDVKLPPQQSRRTKSLI 270
           D SWY +RF + IG+ATDVK+P +  RR  +++
Sbjct: 276 DISWYIVRFFEIIGLATDVKVPTEAQRRRMAIV 306

BLAST of Csor.00g225840 vs. NCBI nr
Match: KAG6590286.1 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 577 bits (1488), Expect = 3.58e-208
Identity = 277/277 (100.00%), Postives = 277/277 (100.00%), Query Frame = 0

Query: 1   MDTAKPRRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRALRIALALHFITGFFGI 60
           MDTAKPRRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRALRIALALHFITGFFGI
Sbjct: 1   MDTAKPRRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRALRIALALHFITGFFGI 60

Query: 61  TLSYHRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVGMHRYHHQVADTEKDPQRTPR 120
           TLSYHRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVGMHRYHHQVADTEKDPQRTPR
Sbjct: 61  TLSYHRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVGMHRYHHQVADTEKDPQRTPR 120

Query: 121 REFVKRDNVGDLENQSFYKFIHKTYLLHPIGLAILLYVVGGMPFLVWGMGVRTVLVLHQS 180
           REFVKRDNVGDLENQSFYKFIHKTYLLHPIGLAILLYVVGGMPFLVWGMGVRTVLVLHQS
Sbjct: 121 REFVKRDNVGDLENQSFYKFIHKTYLLHPIGLAILLYVVGGMPFLVWGMGVRTVLVLHQS 180

Query: 181 LMVNTICHRFGKRQWKTNDSSRNNGLMSLISFGEGWHNNHHAFPYSARHGFEWWQVDTSW 240
           LMVNTICHRFGKRQWKTNDSSRNNGLMSLISFGEGWHNNHHAFPYSARHGFEWWQVDTSW
Sbjct: 181 LMVNTICHRFGKRQWKTNDSSRNNGLMSLISFGEGWHNNHHAFPYSARHGFEWWQVDTSW 240

Query: 241 YAIRFLQAIGVATDVKLPPQQSRRTKSLIMDNQVLQH 277
           YAIRFLQAIGVATDVKLPPQQSRRTKSLIMDNQVLQH
Sbjct: 241 YAIRFLQAIGVATDVKLPPQQSRRTKSLIMDNQVLQH 277

BLAST of Csor.00g225840 vs. NCBI nr
Match: XP_022961086.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 548 bits (1411), Expect = 6.89e-196
Identity = 272/311 (87.46%), Postives = 272/311 (87.46%), Query Frame = 0

Query: 1   MDTAKPRRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRALRIALALHFITGFFGI 60
           M TAKPRRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSR LRIALALHFITGFFGI
Sbjct: 1   MYTAKPRRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRVLRIALALHFITGFFGI 60

Query: 61  TLSYHRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVGMHRYHHQVADTEKDPQRTPR 120
           TLSYHRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWV MHRYHHQVADTEKDPQRTPR
Sbjct: 61  TLSYHRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVSMHRYHHQVADTEKDPQRTPR 120

Query: 121 R----------------------------------EFVKRDNVGDLENQSFYKFIHKTYL 180
           R                                  EFVKRDNVGDLENQSFYKFIHKTYL
Sbjct: 121 RFLWLSNYFASTKRVGPRYLFILEGIEKNIFLMIREFVKRDNVGDLENQSFYKFIHKTYL 180

Query: 181 LHPIGLAILLYVVGGMPFLVWGMGVRTVLVLHQSLMVNTICHRFGKRQWKTNDSSRNNGL 240
           LHPIGLAILLYVVGGMPFLVWGMGVRTVLVLH SLMVNTICHRFGKRQWKTNDSSRNNGL
Sbjct: 181 LHPIGLAILLYVVGGMPFLVWGMGVRTVLVLHLSLMVNTICHRFGKRQWKTNDSSRNNGL 240

Query: 241 MSLISFGEGWHNNHHAFPYSARHGFEWWQVDTSWYAIRFLQAIGVATDVKLPPQQSRRTK 277
           MSLISFGEGWHNNHHAFPYSARHGFEWWQVDTSWYAIRFLQAIGVATDVKLPP QSRRTK
Sbjct: 241 MSLISFGEGWHNNHHAFPYSARHGFEWWQVDTSWYAIRFLQAIGVATDVKLPPPQSRRTK 300

BLAST of Csor.00g225840 vs. NCBI nr
Match: XP_022988176.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 536 bits (1380), Expect = 4.72e-191
Identity = 262/311 (84.24%), Postives = 267/311 (85.85%), Query Frame = 0

Query: 1   MDTAKPRRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRALRIALALHFITGFFGI 60
           MDTAKP RSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRA+R+ALALHFITGFFGI
Sbjct: 8   MDTAKPPRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRAIRVALALHFITGFFGI 67

Query: 61  TLSYHRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVGMHRYHHQVADTEKDPQRTPR 120
           TLSYHRNLSHRSFKLPKW EYLFALCG HTLLGDPINWV MHRYHHQVADTEKDPQRTPR
Sbjct: 68  TLSYHRNLSHRSFKLPKWFEYLFALCGVHTLLGDPINWVSMHRYHHQVADTEKDPQRTPR 127

Query: 121 R----------------------------------EFVKRDNVGDLENQSFYKFIHKTYL 180
           R                                  +FVKRDNVGDLENQSFYKFIHKTYL
Sbjct: 128 RFLWLSNYFASTKRVGPRYLCILQGIEKNIFLMIRKFVKRDNVGDLENQSFYKFIHKTYL 187

Query: 181 LHPIGLAILLYVVGGMPFLVWGMGVRTVLVLHQSLMVNTICHRFGKRQWKTNDSSRNNGL 240
           LHPIGLAILLYVVGGMPFLVWGMGVRTVLVLH SLMVNTICHRFGKR WKTNDSSRNNGL
Sbjct: 188 LHPIGLAILLYVVGGMPFLVWGMGVRTVLVLHLSLMVNTICHRFGKRSWKTNDSSRNNGL 247

Query: 241 MSLISFGEGWHNNHHAFPYSARHGFEWWQVDTSWYAIRFLQAIGVATDVKLPPQQSRRTK 277
           M LISFGEGWHNNHHAFPYSARHGFEWWQ+DTSWYAIRFLQAIGVATDVKLPP QSRRTK
Sbjct: 248 MGLISFGEGWHNNHHAFPYSARHGFEWWQIDTSWYAIRFLQAIGVATDVKLPPPQSRRTK 307

BLAST of Csor.00g225840 vs. NCBI nr
Match: XP_023533044.1 (acyl-CoA C20 Delta5-desaturase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 532 bits (1370), Expect = 2.87e-188
Identity = 264/311 (84.89%), Postives = 267/311 (85.85%), Query Frame = 0

Query: 1   MDTAKPRRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRALRIALALHFITGFFGI 60
           MDT  P RSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRAL+IALALHFITGFFG+
Sbjct: 88  MDTPNPPRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRALQIALALHFITGFFGV 147

Query: 61  TLSYHRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVGMHRYHHQVADTEKDPQRTPR 120
           TLSYHRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWV MHRYHHQVADTEKDPQRTPR
Sbjct: 148 TLSYHRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVSMHRYHHQVADTEKDPQRTPR 207

Query: 121 R----------------------------------EFVKRDNVGDLENQSFYKFIHKTYL 180
           R                                  EFVKRDNVGDLENQSFYKFIHKTYL
Sbjct: 208 RFLWLSNYFGSTKRVGPRYLCILEGIEKNIFLMIREFVKRDNVGDLENQSFYKFIHKTYL 267

Query: 181 LHPIGLAILLYVVGGMPFLVWGMGVRTVLVLHQSLMVNTICHRFGKRQWKTNDSSRNNGL 240
           LHPIGLAILLYVVGGMP LVWGMGVRTVLVLH SLMVNTICHRFGKRQWKTNDSSRNNGL
Sbjct: 268 LHPIGLAILLYVVGGMPSLVWGMGVRTVLVLHLSLMVNTICHRFGKRQWKTNDSSRNNGL 327

Query: 241 MSLISFGEGWHNNHHAFPYSARHGFEWWQVDTSWYAIRFLQAIGVATDVKLPPQQSRRTK 277
           MSLISFGEGWHNNHHAF YSARHGFEWWQVDTSWYAIRFLQAIGVA+DVKLPP QSRRTK
Sbjct: 328 MSLISFGEGWHNNHHAFLYSARHGFEWWQVDTSWYAIRFLQAIGVASDVKLPPPQSRRTK 387

BLAST of Csor.00g225840 vs. NCBI nr
Match: XP_038879870.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 390 bits (1001), Expect = 2.06e-133
Identity = 192/299 (64.21%), Postives = 220/299 (73.58%), Query Frame = 0

Query: 5   KPRRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRALRIALALHFITGFFGITLSY 64
           KP +SSGKEEW +IDK+ A  IFL+HVLCIFAPFQ N  AL +A  L+FI+G FG++LSY
Sbjct: 12  KPLKSSGKEEWIEIDKEMAFIIFLLHVLCIFAPFQFNWSALWVATMLYFISGLFGLSLSY 71

Query: 65  HRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVGMHRYHHQVADTEKDPQR------- 124
           HRNL+HRSFKLPKWLEYLFA CG   L G+PI+WV MHRYHH+VADT+KDPQR       
Sbjct: 72  HRNLAHRSFKLPKWLEYLFAYCGVQALEGNPIDWVSMHRYHHEVADTDKDPQRFDQRFSW 131

Query: 125 ------------TPR----------------REFVKRDNVGDLENQSFYKFIHKTYLLHP 184
                        PR                R+ V+RDNV DLE Q FYKFIHKTYLLHP
Sbjct: 132 FPNYYASTKRVNRPRYFVIFQEIEKNIFLMVRKQVRRDNVRDLEKQIFYKFIHKTYLLHP 191

Query: 185 IGLAILLYVVGGMPFLVWGMGVRTVLVLHQSLMVNTICHRFGKRQWKTNDSSRNNGLMSL 244
           I LAILLYVVGGMPFL+WGMGVR V+VLH +LMVN+ICH  GK+QWKT D S N G +SL
Sbjct: 192 IALAILLYVVGGMPFLIWGMGVRNVVVLHTTLMVNSICHGSGKQQWKTRDLSLNVGWVSL 251

Query: 245 ISFGEGWHNNHHAFPYSARHGFEWWQVDTSWYAIRFLQAIGVATDVKLPPQQSRRTKSL 268
           ISFGE WHNNHHAFPYSARHGF+WWQ+D SWY I FLQ IGVAT+VKL P ++   + L
Sbjct: 252 ISFGESWHNNHHAFPYSARHGFKWWQIDFSWYVINFLQVIGVATNVKLAPHKAIHKQKL 310

BLAST of Csor.00g225840 vs. ExPASy TrEMBL
Match: A0A6J1H9D0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111461702 PE=3 SV=1)

HSP 1 Score: 548 bits (1411), Expect = 3.34e-196
Identity = 272/311 (87.46%), Postives = 272/311 (87.46%), Query Frame = 0

Query: 1   MDTAKPRRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRALRIALALHFITGFFGI 60
           M TAKPRRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSR LRIALALHFITGFFGI
Sbjct: 1   MYTAKPRRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRVLRIALALHFITGFFGI 60

Query: 61  TLSYHRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVGMHRYHHQVADTEKDPQRTPR 120
           TLSYHRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWV MHRYHHQVADTEKDPQRTPR
Sbjct: 61  TLSYHRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVSMHRYHHQVADTEKDPQRTPR 120

Query: 121 R----------------------------------EFVKRDNVGDLENQSFYKFIHKTYL 180
           R                                  EFVKRDNVGDLENQSFYKFIHKTYL
Sbjct: 121 RFLWLSNYFASTKRVGPRYLFILEGIEKNIFLMIREFVKRDNVGDLENQSFYKFIHKTYL 180

Query: 181 LHPIGLAILLYVVGGMPFLVWGMGVRTVLVLHQSLMVNTICHRFGKRQWKTNDSSRNNGL 240
           LHPIGLAILLYVVGGMPFLVWGMGVRTVLVLH SLMVNTICHRFGKRQWKTNDSSRNNGL
Sbjct: 181 LHPIGLAILLYVVGGMPFLVWGMGVRTVLVLHLSLMVNTICHRFGKRQWKTNDSSRNNGL 240

Query: 241 MSLISFGEGWHNNHHAFPYSARHGFEWWQVDTSWYAIRFLQAIGVATDVKLPPQQSRRTK 277
           MSLISFGEGWHNNHHAFPYSARHGFEWWQVDTSWYAIRFLQAIGVATDVKLPP QSRRTK
Sbjct: 241 MSLISFGEGWHNNHHAFPYSARHGFEWWQVDTSWYAIRFLQAIGVATDVKLPPPQSRRTK 300

BLAST of Csor.00g225840 vs. ExPASy TrEMBL
Match: A0A6J1JGI0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111485493 PE=3 SV=1)

HSP 1 Score: 536 bits (1380), Expect = 2.29e-191
Identity = 262/311 (84.24%), Postives = 267/311 (85.85%), Query Frame = 0

Query: 1   MDTAKPRRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRALRIALALHFITGFFGI 60
           MDTAKP RSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRA+R+ALALHFITGFFGI
Sbjct: 8   MDTAKPPRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRAIRVALALHFITGFFGI 67

Query: 61  TLSYHRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVGMHRYHHQVADTEKDPQRTPR 120
           TLSYHRNLSHRSFKLPKW EYLFALCG HTLLGDPINWV MHRYHHQVADTEKDPQRTPR
Sbjct: 68  TLSYHRNLSHRSFKLPKWFEYLFALCGVHTLLGDPINWVSMHRYHHQVADTEKDPQRTPR 127

Query: 121 R----------------------------------EFVKRDNVGDLENQSFYKFIHKTYL 180
           R                                  +FVKRDNVGDLENQSFYKFIHKTYL
Sbjct: 128 RFLWLSNYFASTKRVGPRYLCILQGIEKNIFLMIRKFVKRDNVGDLENQSFYKFIHKTYL 187

Query: 181 LHPIGLAILLYVVGGMPFLVWGMGVRTVLVLHQSLMVNTICHRFGKRQWKTNDSSRNNGL 240
           LHPIGLAILLYVVGGMPFLVWGMGVRTVLVLH SLMVNTICHRFGKR WKTNDSSRNNGL
Sbjct: 188 LHPIGLAILLYVVGGMPFLVWGMGVRTVLVLHLSLMVNTICHRFGKRSWKTNDSSRNNGL 247

Query: 241 MSLISFGEGWHNNHHAFPYSARHGFEWWQVDTSWYAIRFLQAIGVATDVKLPPQQSRRTK 277
           M LISFGEGWHNNHHAFPYSARHGFEWWQ+DTSWYAIRFLQAIGVATDVKLPP QSRRTK
Sbjct: 248 MGLISFGEGWHNNHHAFPYSARHGFEWWQIDTSWYAIRFLQAIGVATDVKLPPPQSRRTK 307

BLAST of Csor.00g225840 vs. ExPASy TrEMBL
Match: A0A6J1GWB1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111458011 PE=3 SV=1)

HSP 1 Score: 355 bits (910), Expect = 3.90e-120
Identity = 168/280 (60.00%), Postives = 199/280 (71.07%), Query Frame = 0

Query: 5   KPRRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRALRIALALHFITGFFGITLSY 64
           KP  S GK++W KIDK+ A   F +H LCIFAPFQ+N  A+ I+  L+ I G FGIT+SY
Sbjct: 12  KPPMSFGKKKWTKIDKKVAFTFFFLHFLCIFAPFQINWSAISISFTLYIIIGLFGITISY 71

Query: 65  HRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVGMHRYHHQVADTEKDPQRTPRREFV 124
           HRNLSH+SFK+PKWLEYLFA CG H   GDPINWV  HRYHHQ  DTEKDP  +P + F 
Sbjct: 72  HRNLSHKSFKIPKWLEYLFAYCGVHAFQGDPINWVSTHRYHHQFVDTEKDPH-SPIKGFW 131

Query: 125 -----------------------KRDNVGDLENQSFYKFIHKTYLLHPIGLAILLYVVGG 184
                                  K DN  DLENQ+FY+FIHKTY LHPI LA++LY +GG
Sbjct: 132 SSHIIWLLYKGDGSLSEMLGRHRKWDNACDLENQTFYRFIHKTYFLHPIALALILYGIGG 191

Query: 185 MPFLVWGMGVRTVLVLHQSLMVNTICHRFGKRQWKTNDSSRNNGLMSLISFGEGWHNNHH 244
            P+ +WGM VR+V+ LH + MVN++CH +GK+QWKTND SRNN  +SL+ FGEGWHNNHH
Sbjct: 192 FPYFIWGMCVRSVIFLHGTFMVNSVCHIWGKQQWKTNDLSRNNWWISLLVFGEGWHNNHH 251

Query: 245 AFPYSARHGFEWWQVDTSWYAIRFLQAIGVATDVKLPPQQ 261
           AF YSAR G EWWQ D  WY IRFL+AIGVAT VKLP Q+
Sbjct: 252 AFEYSARLGIEWWQYDPGWYVIRFLEAIGVATHVKLPSQR 290

BLAST of Csor.00g225840 vs. ExPASy TrEMBL
Match: A0A6J1DIY2 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111020911 PE=3 SV=1)

HSP 1 Score: 349 bits (895), Expect = 2.70e-117
Identity = 170/296 (57.43%), Postives = 202/296 (68.24%), Query Frame = 0

Query: 9   SSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRALRIALALHFITGFFGITLSYHRNL 68
           S G+ +W K+DK  A  +  +HVLCIFAPFQ N  A  IA AL+ ITG FGIT+SYHRNL
Sbjct: 18  SFGRRKWTKMDKTNACILLFLHVLCIFAPFQFNWSAFWIAFALYIITGLFGITISYHRNL 77

Query: 69  SHRSFKLPKWLEYLFALCGAHTLLGDPINWVGMHRYHHQVADTEKDP------------- 128
           SHRSFKLPKWLEYLFA CG  +L GDPI WV  HRYHHQ  DTEKDP             
Sbjct: 78  SHRSFKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHQFVDTEKDPHSPIQGFWFSYIF 137

Query: 129 ---------------QRTPRR-------EFVKRDNVGDLENQSFYKFIHKTYLLHPIGLA 188
                          Q  P R       +  + DNVGDL+ Q+FY FIH TY+LHPI LA
Sbjct: 138 WIFDSNTLTNKMSNFQERPDRSTSVMIMKHNRLDNVGDLKKQTFYTFIHNTYILHPIALA 197

Query: 189 ILLYVVGGMPFLVWGMGVRTVLVLHQSLMVNTICHRFGKRQWKTNDSSRNNGLMSLISFG 248
           ++LY  GG+PFL+WGM VR+V++LH + +VN+ICH +GK+ WKT D SRNN L++LI+FG
Sbjct: 198 VILYAGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGKQPWKTGDLSRNNWLINLIAFG 257

Query: 249 EGWHNNHHAFPYSARHGFEWWQVDTSWYAIRFLQAIGVATDVKLPPQQSRRTKSLI 269
           EGWHNNHHAF YSAR GFEWWQ D  WY + FLQAIG+AT VKLP Q  ++  SLI
Sbjct: 258 EGWHNNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLATQVKLPSQSHKQKLSLI 313

BLAST of Csor.00g225840 vs. ExPASy TrEMBL
Match: A0A1S3BY39 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103494692 PE=3 SV=1)

HSP 1 Score: 343 bits (880), Expect = 1.46e-115
Identity = 167/283 (59.01%), Postives = 194/283 (68.55%), Query Frame = 0

Query: 1   MDTAKPRRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRALRIALALHFITGFFGI 60
           M T KP  S GK +  KIDK+ A    L+H+LCIFAPFQ+N  A  I L L  IT  FGI
Sbjct: 9   MKTVKPPMSFGKRKRNKIDKKVASVFLLLHLLCIFAPFQINWGAFSICLVLFTITSLFGI 68

Query: 61  TLSYHRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVGMHRYHHQVADTEKDPQRTP- 120
           T+SYHRNLSH+SF++PKWLEYLFA CG H L G+PINWV  HRYHHQ  DTE+DP  TP 
Sbjct: 69  TISYHRNLSHKSFQIPKWLEYLFAYCGVHALQGNPINWVSTHRYHHQFVDTEQDPH-TPI 128

Query: 121 ----------------------RREFVKRDNVGDLENQSFYKFIHKTYLLHPIGLAILLY 180
                                  R+  K DN  DLE Q FY+FI KTYLLHPI LAI+LY
Sbjct: 129 HGFWFGYIIWILEEKDGSIAGMNRKHRKLDNASDLEKQIFYRFIRKTYLLHPIALAIILY 188

Query: 181 VVGGMPFLVWGMGVRTVLVLHQSLMVNTICHRFGKRQWKTNDSSRNNGLMSLISFGEGWH 240
           V+GG PF +WG  VRTV++LH + MVN+ CH +G + WKTND S NN  +SL++FGEGWH
Sbjct: 189 VIGGFPFFIWGTCVRTVMLLHSTFMVNSFCHMWGNQPWKTNDFSANNWWVSLVTFGEGWH 248

Query: 241 NNHHAFPYSARHGFEWWQVDTSWYAIRFLQAIGVATDVKLPPQ 260
           NNHHAF YSAR G EWWQ D  WY I FLQAIG+AT+VKLP Q
Sbjct: 249 NNHHAFEYSARLGIEWWQFDIGWYVIMFLQAIGLATNVKLPSQ 290

BLAST of Csor.00g225840 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 316.2 bits (809), Expect = 2.6e-86
Identity = 142/277 (51.26%), Postives = 189/277 (68.23%), Query Frame = 0

Query: 12  KEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRALRIALALHFITGFFGITLSYHRNLSHR 71
           + EW  +D      +  MH+L + APFQ N RA+ +A  L+ +TG  GITLS+HRNLSH+
Sbjct: 95  EREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHK 154

Query: 72  SFKLPKWLEYLFALCGAHTLLGDPINWVGMHRYHHQVADTEKDPQ--------------- 131
           +FKLPKWLEYLFA CGA  L G+PI+WV  HRYHHQ  D+++DP                
Sbjct: 155 AFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMF 214

Query: 132 --RTPRREFVKRDNVGDLENQSFYKFIHKTYLLHPIGLAILLYVVGGMPFLVWGMGVRTV 191
              T  +   + +NVGDLE Q FY+F+  TY+LHP+ LA+ LY +GG PF+VWGMGVR V
Sbjct: 215 DTNTITQRCGEPNNVGDLEKQPFYRFLRTTYILHPLALAVALYAMGGFPFIVWGMGVRIV 274

Query: 192 LVLHQSLMVNTICHRFGKRQWKTNDSSRNNGLMSLISFGEGWHNNHHAFPYSARHGFEWW 251
            V H + +VN+ CH +GK+ W T D S+NN  ++ ++FGEGWHNNHHAF +SARHG EWW
Sbjct: 275 WVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAFGEGWHNNHHAFEFSARHGLEWW 334

Query: 252 QVDTSWYAIRFLQAIGVATDVKLPPQQSRRTKSLIMD 272
           Q+D +WY ++FLQAIG+ATDVKLP +  ++  +   D
Sbjct: 335 QLDMTWYVVKFLQAIGLATDVKLPSEAQKQRMAFTSD 371

BLAST of Csor.00g225840 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 288.1 bits (736), Expect = 7.4e-78
Identity = 134/286 (46.85%), Postives = 183/286 (63.99%), Query Frame = 0

Query: 2   DTAKPRRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRALRIALALHFITGFFGIT 61
           +  + +R+  + +W ++D  KA     +H LC+ APF     ALR+AL ++ + G  GIT
Sbjct: 21  EMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGG-LGIT 80

Query: 62  LSYHRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVGMHRYHHQVADTEKDPQRTPRR 121
           +SYHRNL+HRSFK+PKWLEY FA CG   + GDPI+WV  HRYHHQ  D+++DP  +P  
Sbjct: 81  VSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDP-HSPNE 140

Query: 122 EF------------------VKRDNVGDLENQSFYKFIHKTYLLHPIGLAILLYVVGGMP 181
            F                   +R NV DL+ Q +YKF+ +T L H +    LLY  GG+ 
Sbjct: 141 GFWFSHLLWLFDTGYLVEKCGRRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLS 200

Query: 182 FLVWGMGVRTVLVLHQSLMVNTICHRFGKRQWKTNDSSRNNGLMSLISFGEGWHNNHHAF 241
           FL WGMG+   +  H + ++N++CH +G R WKTND+SRN   +S+ SFGE WHNNHHAF
Sbjct: 201 FLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRNVWWLSVFSFGESWHNNHHAF 260

Query: 242 PYSARHGFEWWQVDTSWYAIRFLQAIGVATDVKLPPQQSRRTKSLI 270
             SAR G EWWQ+D SWY +RFL+ IG+ATDVKLP +  RR  +++
Sbjct: 261 ESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQRRRMAMV 304

BLAST of Csor.00g225840 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 275.0 bits (702), Expect = 6.5e-74
Identity = 131/273 (47.99%), Postives = 173/273 (63.37%), Query Frame = 0

Query: 15  WAKIDKQKAIFIFLMHVLCIFAPFQVNSRALRIALALHFITGFFGITLSYHRNLSHRSFK 74
           W ++D  K    F +H L + APF     AL +   L +  G  GIT+SYHRNL+HRSFK
Sbjct: 36  WRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTF-LFYTIGGLGITVSYHRNLAHRSFK 95

Query: 75  LPKWLEYLFALCGAHTLLGDPINWVGMHRYHHQVADTEKDPQRTPRREF----------- 134
           +PKWLEYL A C    + GDPI+WV  HRYHHQ  D+E+DP  +P+  F           
Sbjct: 96  VPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDP-HSPKEGFWFSHLLWIYDS 155

Query: 135 -------VKRDNVGDLENQSFYKFIHKTYLLHPIGLAILLYVVGGMPFLVWGMGVRTVLV 194
                   +R NV DL+ Q FY+F+ KT L H +GL   L+ +GGM F+ WGMGV   L 
Sbjct: 156 AYLVSKCGRRANVEDLKRQWFYRFLQKTVLFHILGLGFFLFYLGGMSFVTWGMGVGAALE 215

Query: 195 LHQSLMVNTICHRFGKRQWKTNDSSRNNGLMSLISFGEGWHNNHHAFPYSARHGFEWWQV 254
           +H + ++N++CH +G R WKTND+SRN   +S+ SFGE WHNNHHAF  SAR G EWWQ+
Sbjct: 216 VHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQI 275

Query: 255 DTSWYAIRFLQAIGVATDVKLPPQQSRRTKSLI 270
           D SWY +RF + IG+ATDVK+P +  RR  +++
Sbjct: 276 DISWYIVRFFEIIGLATDVKVPTEAQRRRMAIV 306

BLAST of Csor.00g225840 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 265.0 bits (676), Expect = 6.8e-71
Identity = 130/283 (45.94%), Postives = 175/283 (61.84%), Query Frame = 0

Query: 5   KPRRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRALRIALALHFITGFFGITLSY 64
           + +RS  +  W   D  K +     H+L + APF  +  A  +   L FI G   ITLSY
Sbjct: 81  RTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGIC-ITLSY 140

Query: 65  HRNLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVGMHRYHHQVADTEKDPQRTPRREF- 124
           HRNLSHRSF LPKWLEYLFA  G     GDPI WV  HRYHH+  +T++DP  +P + F 
Sbjct: 141 HRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDP-HSPTQGFW 200

Query: 125 -----------------VKRDNVGDLENQSFYKFIHKTYLLHPIGLAILLYVVGGMPFLV 184
                               +NV DL  Q FY+F+ +T LLH +  + L Y  GGMP LV
Sbjct: 201 FSHMAWIFDTSSILENCGGEENVDDLVRQPFYRFLQRTVLLHMMAYSFLFYFCGGMPLLV 260

Query: 185 WGMGVRTVLVLHQSLMVNTICHRFGKRQWKTNDSSRNNGLMSLISFGEGWHNNHHAFPYS 244
           WG+G+   + LH + +VN++CH +G R W T+D S+NN  +++++ GEGWHNNHHAF +S
Sbjct: 261 WGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFSKNNWWVAILTLGEGWHNNHHAFEFS 320

Query: 245 ARHGFEWWQVDTSWYAIRFLQAIGVATDVKLPPQQSRRTKSLI 270
           ARHG EWWQ+D +W  IRFL+AIG+AT+VKLP +   + K+L+
Sbjct: 321 ARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTETQMKGKALV 361

BLAST of Csor.00g225840 vs. TAIR 10
Match: AT1G06350.1 (Fatty acid desaturase family protein )

HSP 1 Score: 252.3 bits (643), Expect = 4.5e-67
Identity = 121/281 (43.06%), Postives = 174/281 (61.92%), Query Frame = 0

Query: 7   RRSSGKEEWAKIDKQKAIFIFLMHVLCIFAPFQVNSRALRIALALHFITGFFGITLSYHR 66
           +R+  + +W  +D  +A  + ++H LC+ APF     ALR  L L  +T    IT S+HR
Sbjct: 21  KRAYFQRQWPLVDVVRASVVVIVHFLCLLAPFNFKWEALRFGLVLFALT-TLSITFSFHR 80

Query: 67  NLSHRSFKLPKWLEYLFALCGAHTLLGDPINWVGMHRYHHQVADTEKDPQRTPRR----- 126
           NLSHRSFK+PKWLEY +A      L GDP++WV +HR+HHQ  D+++DP  +P+      
Sbjct: 81  NLSHRSFKIPKWLEYPWAYSAVFALQGDPMDWVSIHRFHHQFTDSDRDP-HSPKEGLLFS 140

Query: 127 --------EFVK-----RDNVGDLENQSFYKFIHKTYLLHPIGLAILLYVVGGMPFLVWG 186
                   +++K     RDNV DL+ Q FYKF+ +T  +H +    +LY+ GG+P+L  G
Sbjct: 141 HILWIFDTQYIKYKCGGRDNVLDLKKQWFYKFLRRTIAVHILMFWTILYLYGGLPYLTCG 200

Query: 187 MGVRTVLVLHQSLMVNTICHRFGKRQWKTNDSSRNNGLMSLISFGEGWHNNHHAFPYSAR 246
            GV   +  H + +VN+ CH +G R W T D+SRN   +SL + GE WHNNHHAF  SAR
Sbjct: 201 GGVGIFIGYHVTWLVNSACHIWGSRSWNTKDTSRNVWWLSLFTMGESWHNNHHAFESSAR 260

Query: 247 HGFEWWQVDTSWYAIRFLQAIGVATDVKLPPQQSRRTKSLI 270
            G EWWQ+D +WY IR  + +G+ATDVKLP +  ++  +L+
Sbjct: 261 QGLEWWQIDITWYLIRLFEVLGIATDVKLPSELQKQKMALV 299

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949X03.6e-8551.26Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab... [more]
O657971.0e-7646.85Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=... [more]
P0DOW22.6e-7547.33Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1[more]
P0DOW32.2e-7446.37Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1[more]
Q9SID29.2e-7347.99Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
KAG6590286.13.58e-208100.00Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic, partia... [more]
XP_022961086.16.89e-19687.46palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C... [more]
XP_022988176.14.72e-19184.24palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C... [more]
XP_023533044.12.87e-18884.89acyl-CoA C20 Delta5-desaturase-like [Cucurbita pepo subsp. pepo][more]
XP_038879870.12.06e-13364.21palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [B... [more]
Match NameE-valueIdentityDescription
A0A6J1H9D03.34e-19687.46palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1JGI02.29e-19184.24palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1GWB13.90e-12060.00palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1DIY22.70e-11757.43palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A1S3BY391.46e-11559.01palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
Match NameE-valueIdentityDescription
AT3G15850.12.6e-8651.26fatty acid desaturase 5 [more]
AT1G06080.17.4e-7846.85delta 9 desaturase 1 [more]
AT2G31360.16.5e-7447.9916:0delta9 desaturase 2 [more]
AT3G15870.16.8e-7145.94Fatty acid desaturase family protein [more]
AT1G06350.14.5e-6743.06Fatty acid desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 145..241
e-value: 1.9E-10
score: 41.0
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 13..116
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 126..266
IPR015876Acyl-CoA desaturaseCDDcd03505Delta9-FADS-likecoord: 47..256
e-value: 2.84896E-65
score: 200.09
NoneNo IPR availablePANTHERPTHR11351:SF94BNAC05G37460D PROTEINcoord: 13..116
coord: 126..266

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g225840.m01Csor.00g225840.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water