Csor.00g224620 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g224620
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionnon-specific phospholipase C2-like
LocationCsor_Chr10: 4602828 .. 4605625 (+)
RNA-Seq ExpressionCsor.00g224620
SyntenyCsor.00g224620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCCAAATCCACTGCCTTCTTCCTTCTCCTATTGGCCTTCTACACTCCCCTCCTTCATGGCTCCTCCGTCAAGACCATTATCGTTCTCATCATGGAGAATTGCTTCTTCGATCACATGCTCGGCTGGATGAAGAAACTCAACCTTAAAATCAATGGCGTCGATGGCACTGAATCCAATTTCCTCAACGCCAGTGATCAGAAATTGAAACAGTTTTTATTCAAGGACCAGGCTCACTATGTCGACCCCGACCCTGTCCAATCCTTTCAAGTCATACGAGAGAAGATTTTGGGCTCTGATAACACCTCTGCTAATCCCCCGCGGATGAACAGATTCGCTCAGAAGGCATTCTCCATGGACAACACCGCCGCCATGTCTGCCGATGTAATGAATGAATTTCAACCCGATTTGGTCGTCGTCTACAAGTCTCTCGTCTCCGTTTTTGCGGTTTTCGACAGGTGATTTTGTCGTAATTTCCTTTTCGATTGTAGATTGGTATTTGTGTGTTCTGAAATTGAAAGATCTGGAAATTGCAATTATCAGTCGATTAGTTTGATTGTAAACGAAAATCGGAATGTGCAAACTGATTTTATAGGTGGTTTGCATCGGCACCGACCTCAACGATGTCGAATCGGCTCTACATTCACTCAGGAACCTCCGCTGGAGCCACGAGTAACATTCCGTCATTCCTTGGCAAAAGCTATCCACAGCGAAAGATACGATGCCGGAACGTCCTTCGGAATCTACTTCCAGAACCTTCCGTCGACGCTGGCCTACGGAAATCTACGAAAACTGAAATACCTAAACAAATTTCATCTGTTTGATTTGGACTTCAAGAGGCACGCAAATCAGGGGAAATTACCGAACTACGTGGTGCTGGAGCCGCAGTACTTCAATTTACCGCTCGAGCTAGGGCACGATGATCATCCATCGCACGATGTGTACCAAGGACAAATGCTCATCAAGGAGGTTTACGAGACGCTCAGATCTTCGCCGCAGTGGATCGAAACTCTGTTCATCATCACCTACGATGAGCACGGTGGATTCTACGACCACGTTCCAATGCCGGTCACCGACGTTCCTAGCCCCGACGGAATCGTCGAGCCGAAGCTATTTCTTTTTCGTTTTGATCGGCTTGGAATTCGAGTTTCGACCATCATGATCTCGCCGTGGATAGAGAAGGGCCCTAGTATGAATTCTTTATTAGTTCCTTTTATTATTATCATTTTTTTTTTTTCACAATTTAGAAATTTGTTTCACAATATGTTTGAAATTTAAATCACAATTTTTTATTTATTTATCAAATTTCCTTGTTAATTTTTTAAAAACTATTTCAAAACTAGTTTACAAACAAAATATTATTTATAATTTTTTTTCAATGAGGACATTCATTGTATTTTTCAATTTTATCTAATAATATTAATAATTATAAATTTAATTTCAATAATTTCGATAAATCTTATAATATTCAATTTCTTTTATTGTCGTATCTATAACTCTATATTTTTCTTTGTACATCTCCAATAGTATATATACAATAGGAAAACTTGAAGGGTACAATAGGAAAATTGAAGGGTAGGGAAAATGTAAGTTAGAAAAAAATAAATTTAGGTCTAAAGAGGGAAAATTGTGACAATGGAGTAAAGGACGATAACGATTATAGGATTCTCCTTTTTCGGTCCCAAATCTAAGCCCACTGCTAGCAGATATCGTCTTCTTTTAGCTTTTCTTTTTGGGTTTTCCCTCGAGATTTTTAAAATGCGTGCTAGGGAGAGGTTTAACACAGTTATAAAGAATGTTTTGTTCCCCTCTCCAACCAATATGGGATCTCACGATTCACCCCCTTGAGTCTCAGTGTCCTCGCTGGCACACTACCCGATGTTTGGCTCTAATACCATTTGTAACAATTCAAGCCCACCACTTGCCGATATTGTTTGATTGTGAGATCTCACATCGATGAGAGAGAAGAAACGAAACATTCTTTATAAGGATGTGAAAACCTTTCCCTAGGAAATGCATTTTAAAAACCTTGAGAGAAAACCCGAAAAGAAAAACCTAAAGAGGACAATATCTACTAGCGGTGGCTTAAGCTGTTACAAATTTTATGTTGGGATGCCTTTTAAAAATTTTCTTTAGATGCACGTGAACGAGATAAAACATGCTAGAAGAAGTTGTGTTGGTTTATGGGGAAAGTTTTAAATCTTAAGAGGTGAGGAATATGTAACATGCCCATATCACAGAAGATACAGAGAAATGTCGGAACTATCATATATTGTATCTGTATTCCAAATCCTAGACATGGATGGTTACAATATTTAGGTTTATGAAAATATGGCTAAGGGTTGAGAGGTTAACAACATGTTTTATGAGTACGAACACTCCTCCATTCCCGCCACCCCCATCACCGTCAAGAAGATCTTCAACCTCCCATCTCCCTTCCTCACCAAGAGGGACAAGTGGGCCAGATCCTTCAAGTTCATCCTCCCAAACCCGACCTCCCCGAGAACCGACTGTCCCGGTGAGTCCAAAACATCAATCTTACTATAAATCAAACCGGACGTATCATTTTTTATGATCTATTTAAACTTACAAATCTATCTATCAATTTCGATATAGAACAACTCCCAGCACCAGTAAACATCAGGAACAGCCCGGCGAACAAATCAGCCATGCTCACCGAGTTTCAGCAGGAGCTGATGCAGTTGGCTATAGCGTTGAAAGGAGAGTATATCCTCAGTAGTTACCCTGAACCCTTGGGAAATGATATGACTGTGAAGGAAGGTAGGAATATATAA

mRNA sequence

ATGGCGGCCAAATCCACTGCCTTCTTCCTTCTCCTATTGGCCTTCTACACTCCCCTCCTTCATGGCTCCTCCGTCAAGACCATTATCGTTCTCATCATGGAGAATTGCTTCTTCGATCACATGCTCGGCTGGATGAAGAAACTCAACCTTAAAATCAATGGCGTCGATGGCACTGAATCCAATTTCCTCAACGCCAGTGATCAGAAATTGAAACAGTTTTTATTCAAGGACCAGGCTCACTATGTCGACCCCGACCCTGTCCAATCCTTTCAAGTCATACGAGAGAAGATTTTGGGCTCTGATAACACCTCTGCTAATCCCCCGCGGATGAACAGATTCGCTCAGAAGGCATTCTCCATGGACAACACCGCCGCCATGTCTGCCGATGTAATGAATGAATTTCAACCCGATTTGGTCGTCGTCTACAAGTCTCTCGTCTCCGTTTTTGCGGTTTTCGACAGATCTGGAAATTGCAATTATCAGTCGATTAGTTTGATTGTAAACGAAAATCGGAATGTGCAAACTGATTTTATAGGTGGTTTGCATCGGCACCGACCTCAACGATGTCGAATCGGCTCTACATTCACTCAGGAACCTCCGCTGGAGCCACGAGTAACATTCCGTCATTCCTTGGCAAAAGCTATCCACAGCGAAAGATACGATGCCGGAACGTCCTTCGGAATCTACTTCCAGAACCTTCCGTCGACGCTGGCCTACGGAAATCTACGAAAACTGAAATACCTAAACAAATTTCATCTGTTTGATTTGGACTTCAAGAGGCACGCAAATCAGGGGAAATTACCGAACTACGTGGTGCTGGAGCCGCAGTACTTCAATTTACCGCTCGAGCTAGGGCACGATGATCATCCATCGCACGATGTGTACCAAGGACAAATGCTCATCAAGGAGGTTTACGAGACGCTCAGATCTTCGCCGCAGTGGATCGAAACTCTGTTCATCATCACCTACGATGAGCACGGTGGATTCTACGACCACGTTCCAATGCCGGTCACCGACGTTCCTAGCCCCGACGGAATCGTCGAGCCGAAGCTATTTCTTTTTCGTTTTGATCGGCTTGGAATTCGAGTTTCGACCATCATGATCTCGCCGTGGATAGAGAAGGGCCCTAAACAACTCCCAGCACCAGTAAACATCAGGAACAGCCCGGCGAACAAATCAGCCATGCTCACCGAGTTTCAGCAGGAGCTGATGCAGTTGGCTATAGCGTTGAAAGGAGAGTATATCCTCAGTAGTTACCCTGAACCCTTGGGAAATGATATGACTGTGAAGGAAGGTAGGAATATATAA

Coding sequence (CDS)

ATGGCGGCCAAATCCACTGCCTTCTTCCTTCTCCTATTGGCCTTCTACACTCCCCTCCTTCATGGCTCCTCCGTCAAGACCATTATCGTTCTCATCATGGAGAATTGCTTCTTCGATCACATGCTCGGCTGGATGAAGAAACTCAACCTTAAAATCAATGGCGTCGATGGCACTGAATCCAATTTCCTCAACGCCAGTGATCAGAAATTGAAACAGTTTTTATTCAAGGACCAGGCTCACTATGTCGACCCCGACCCTGTCCAATCCTTTCAAGTCATACGAGAGAAGATTTTGGGCTCTGATAACACCTCTGCTAATCCCCCGCGGATGAACAGATTCGCTCAGAAGGCATTCTCCATGGACAACACCGCCGCCATGTCTGCCGATGTAATGAATGAATTTCAACCCGATTTGGTCGTCGTCTACAAGTCTCTCGTCTCCGTTTTTGCGGTTTTCGACAGATCTGGAAATTGCAATTATCAGTCGATTAGTTTGATTGTAAACGAAAATCGGAATGTGCAAACTGATTTTATAGGTGGTTTGCATCGGCACCGACCTCAACGATGTCGAATCGGCTCTACATTCACTCAGGAACCTCCGCTGGAGCCACGAGTAACATTCCGTCATTCCTTGGCAAAAGCTATCCACAGCGAAAGATACGATGCCGGAACGTCCTTCGGAATCTACTTCCAGAACCTTCCGTCGACGCTGGCCTACGGAAATCTACGAAAACTGAAATACCTAAACAAATTTCATCTGTTTGATTTGGACTTCAAGAGGCACGCAAATCAGGGGAAATTACCGAACTACGTGGTGCTGGAGCCGCAGTACTTCAATTTACCGCTCGAGCTAGGGCACGATGATCATCCATCGCACGATGTGTACCAAGGACAAATGCTCATCAAGGAGGTTTACGAGACGCTCAGATCTTCGCCGCAGTGGATCGAAACTCTGTTCATCATCACCTACGATGAGCACGGTGGATTCTACGACCACGTTCCAATGCCGGTCACCGACGTTCCTAGCCCCGACGGAATCGTCGAGCCGAAGCTATTTCTTTTTCGTTTTGATCGGCTTGGAATTCGAGTTTCGACCATCATGATCTCGCCGTGGATAGAGAAGGGCCCTAAACAACTCCCAGCACCAGTAAACATCAGGAACAGCCCGGCGAACAAATCAGCCATGCTCACCGAGTTTCAGCAGGAGCTGATGCAGTTGGCTATAGCGTTGAAAGGAGAGTATATCCTCAGTAGTTACCCTGAACCCTTGGGAAATGATATGACTGTGAAGGAAGGTAGGAATATATAA

Protein sequence

MAAKSTAFFLLLLAFYTPLLHGSSVKTIIVLIMENCFFDHMLGWMKKLNLKINGVDGTESNFLNASDQKLKQFLFKDQAHYVDPDPVQSFQVIREKILGSDNTSANPPRMNRFAQKAFSMDNTAAMSADVMNEFQPDLVVVYKSLVSVFAVFDRSGNCNYQSISLIVNENRNVQTDFIGGLHRHRPQRCRIGSTFTQEPPLEPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTLAYGNLRKLKYLNKFHLFDLDFKRHANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQGQMLIKEVYETLRSSPQWIETLFIITYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFDRLGIRVSTIMISPWIEKGPKQLPAPVNIRNSPANKSAMLTEFQQELMQLAIALKGEYILSSYPEPLGNDMTVKEGRNI
Homology
BLAST of Csor.00g224620 vs. ExPASy Swiss-Prot
Match: O81020 (Non-specific phospholipase C2 OS=Arabidopsis thaliana OX=3702 GN=NPC2 PE=2 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 1.6e-111
Identity = 232/496 (46.77%), Postives = 290/496 (58.47%), Query Frame = 0

Query: 1   MAAKSTAFFLLL--LAFYTPLLHGSSVKTIIVLIMENCFFDHMLGWMKKLNLKINGVDGT 60
           M+ K+ A   LL     Y  +   S +KTI+V++MEN  FDHMLGWMKKLN +INGVDG+
Sbjct: 1   MSIKAFALIQLLSVTILYNHVHATSPIKTIVVVVMENRSFDHMLGWMKKLNPEINGVDGS 60

Query: 61  ESNFLNASDQKLKQFLFKDQAHYVDPDPVQSFQVIREKILGSDNTSANPPRMNRFAQKAF 120
           ESN ++ SD   ++  F   +HYVDPDP  SFQ IRE++ GS++TS +PP MN F Q+A+
Sbjct: 61  ESNPVSVSDPSSRKIKFGSGSHYVDPDPGHSFQAIREQVFGSNDTSMDPPPMNGFVQQAY 120

Query: 121 SMDNTAAMSADVMNEFQPDLVVVYKSLVSVFAVFDRSGNCNYQSISLIVNENRN-VQTDF 180
           S D +  MSA VMN F+PD V VYKSLVS FAVFDR     + S+      NR  V +  
Sbjct: 121 SEDPSGNMSASVMNGFEPDKVPVYKSLVSEFAVFDRW----FASVPSSTQPNRMFVHSGT 180

Query: 181 IGGLHRHRPQRCRIGSTFTQEPPLEPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTL 240
             G   + P    +   + Q               + I     D   SFGIY+QN+P+ L
Sbjct: 181 SAGATSNNP--ISLAKGYPQ---------------RTIFDNLDDEEFSFGIYYQNIPAVL 240

Query: 241 AYGNLRKLKYLNKFHLFDLDFKRHANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQG 300
            Y +LRKLKY+ KFH +   FK HA QGKLP Y V+E +Y +  LE   DDHPSHDVYQG
Sbjct: 241 FYQSLRKLKYVFKFHSYGNSFKDHAKQGKLPAYTVIEQRYMDTLLEPASDDHPSHDVYQG 300

Query: 301 QMLIKEVYETLRSSPQWIETLFIITYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFD 360
           Q  IKEVYETLR+SPQW ETL IITYDEHGG++DHVP PV +VPSPDGIV P  FLF+F+
Sbjct: 301 QKFIKEVYETLRASPQWNETLLIITYDEHGGYFDHVPTPVRNVPSPDGIVGPDPFLFQFN 360

Query: 361 RLGIRVSTIMISPWIEKG------------------------------------------ 420
           RLGIRV TI +SPWIEKG                                          
Sbjct: 361 RLGIRVPTIAVSPWIEKGTVVHGPNGSPFPSSEYEHSSIPATVKKLFNLSSPFLTKRDEW 420

Query: 421 ------------------PKQLPAPVNIRNSPANKSAMLTEFQQELMQLAIALKGEYILS 434
                             P+ LP PV IR   AN+ A+LTEFQQEL+QLA  LKG+ +L+
Sbjct: 421 AGTFENILQIRKEPRTDCPETLPEPVKIRMGEANEKALLTEFQQELVQLAAVLKGDNMLT 475

BLAST of Csor.00g224620 vs. ExPASy Swiss-Prot
Match: Q8L7Y9 (Non-specific phospholipase C1 OS=Arabidopsis thaliana OX=3702 GN=NPC1 PE=2 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 2.6e-90
Identity = 201/481 (41.79%), Postives = 265/481 (55.09%), Query Frame = 0

Query: 25  VKTIIVLIMENCFFDHMLGWMKKLNLKINGVDGTESNFLNASDQKLKQFLFKDQAHYVDP 84
           +KTI+V++MEN  FDH+LGW+K    +I+G+ G ESN LN SD   K+    D A +VD 
Sbjct: 38  IKTIVVVVMENRSFDHILGWLKSTRPEIDGLTGKESNPLNVSDPNSKKIFVSDDAVFVDM 97

Query: 85  DPVQSFQVIREKILGSDNTSANPPRMNRFAQKAFSMDNTAAMSADVMNEFQPDLVVVYKS 144
           DP  SFQ IRE+I GS++TS + P+MN FAQ++ SM+    M+ +VM+ F+P+++ VY  
Sbjct: 98  DPGHSFQAIREQIFGSNDTSGD-PKMNGFAQQSESME--PGMAKNVMSGFKPEVLPVYTE 157

Query: 145 LVSVFAVFDRSGNCNYQSISLIVNENR-------------NVQTDFIGGLHRHRPQRCRI 204
           L + F VFDR     + S+      NR             NV+ D + G     PQ    
Sbjct: 158 LANEFGVFDRW----FASVPTSTQPNRFYVHSATSHGCSSNVKKDLVKGF----PQ---- 217

Query: 205 GSTFTQEPPLEPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTLAYGNLRKLKYLNKF 264
                                K I     + G SFGIY+QN+P+T  + +LR+LK+L KF
Sbjct: 218 ---------------------KTIFDSLDENGLSFGIYYQNIPATFFFKSLRRLKHLVKF 277

Query: 265 HLFDLDFKRHANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQGQMLIKEVYETLRSS 324
           H + L FK  A  GKLPNY V+E +YF++ L   +DDHPSHDV  GQ  +KEVYETLRSS
Sbjct: 278 HSYALKFKLDAKLGKLPNYSVVEQRYFDIDLFPANDDHPSHDVAAGQRFVKEVYETLRSS 337

Query: 325 PQWIETLFIITYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFDRLGIRVSTIMISPW 384
           PQW E   +ITYDEHGGFYDHVP PV  VP+PDGI+ P  F F FDRLG+RV T +ISPW
Sbjct: 338 PQWKEMALLITYDEHGGFYDHVPTPVKGVPNPDGIIGPDPFYFGFDRLGVRVPTFLISPW 397

Query: 385 IEKGP----------------KQLPAPV-------------------------NIRNSP- 433
           IEKG                   +PA V                          IR+SP 
Sbjct: 398 IEKGTVIHEPEGPTPHSQFEHSSIPATVKKLFNLKSHFLTKRDAWAGTFEKYFRIRDSPR 457

BLAST of Csor.00g224620 vs. ExPASy Swiss-Prot
Match: Q8H965 (Non-specific phospholipase C6 OS=Arabidopsis thaliana OX=3702 GN=NPC6 PE=2 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.8e-84
Identity = 191/469 (40.72%), Postives = 250/469 (53.30%), Query Frame = 0

Query: 23  SSVKTIIVLIMENCFFDHMLGWMK-KLNLKINGVDGTESNFLNASDQKLKQFLFKDQAHY 82
           S +KT++VL++EN  FDH+LGWMK  +N  INGV G E N +  S Q +    F   A +
Sbjct: 37  SPIKTVVVLVLENRSFDHLLGWMKNSVNPTINGVTGQECNPVPNSTQTI---CFTSDAEF 96

Query: 83  VDPDPVQSFQVIREKILGSDNTSANPPRMNRFAQKAFSMDNTAAMSADVMNEFQPDLVVV 142
           VDPDP  SF+ + +++ GS       P M  F ++A SM     +S  VM  F+P+ V V
Sbjct: 97  VDPDPGHSFEAVEQQVFGSG--PGQIPSMMGFVEQALSMPGN--LSETVMKGFRPEAVPV 156

Query: 143 YKSLVSVFAVFDRSGNCNYQSISLIVNENRNVQTDFIGGLHRHRPQRCRIGSTFTQEPPL 202
           Y  LV  FAVFDR     + SI      NR     F+     H       GST   +  L
Sbjct: 157 YAELVKEFAVFDRW----FSSIPGPTQPNRL----FVYSATSH-------GSTSHVKKQL 216

Query: 203 EPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTLAYGNLRKLKYLNKFHLFDLDFKRH 262
                 + ++  ++HS   D    FGIYFQN+P+TL Y NLR+LKY+   H +DL FK+ 
Sbjct: 217 AQGYP-QKTIFDSLHSNDID----FGIYFQNIPTTLFYRNLRQLKYIFNLHQYDLKFKKD 276

Query: 263 ANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQGQMLIKEVYETLRSSPQWIETLFII 322
           A +GKLP+  V+EP+YF+L     +DDHPSHDV  GQ L+KEVYE LRSSPQW ETL +I
Sbjct: 277 AAKGKLPSLTVIEPRYFDLKGLPANDDHPSHDVANGQKLVKEVYEALRSSPQWNETLLVI 336

Query: 323 TYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFDRLGIRVSTIMISPWIEKG------ 382
           TYDEHGGFYDHV  P   +P+PDG   P    F+FDRLG+RV TIM+SPWI+KG      
Sbjct: 337 TYDEHGGFYDHVKTPYVGIPNPDGNTGPAPGFFKFDRLGVRVPTIMVSPWIQKGTVVSEA 396

Query: 383 -----------------------------------------------------PKQLPAP 432
                                                                P  LP  
Sbjct: 397 KGPTESSEYEHSSIPATIKKLFNLSSNFLTHRDAWAATFEDVVSHLTTPRTDCPMTLPEV 456

BLAST of Csor.00g224620 vs. ExPASy Swiss-Prot
Match: Q9SRQ7 (Non-specific phospholipase C4 OS=Arabidopsis thaliana OX=3702 GN=NPC4 PE=1 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 1.4e-83
Identity = 186/455 (40.88%), Postives = 246/455 (54.07%), Query Frame = 0

Query: 25  VKTIIVLIMENCFFDHMLGWMKKLNLKINGVDGTE--SNFLNASDQKLKQFLFKDQAHYV 84
           +KTI+VL+ EN  FDH LGW K+LN +I+GV  ++  SN +++SD    + +F DQ+ YV
Sbjct: 14  IKTIVVLVQENRSFDHTLGWFKELNREIDGVTKSDPKSNTVSSSDTNSLRVVFGDQSQYV 73

Query: 85  DPDPVQSFQVIREKILGSDNTSANP------PRMNRFAQKAFSMDNTAAMSADVMNEFQP 144
           +PDP  S Q I E++ G    S  P      P M+ FAQ A    N   MS+ VMN F+P
Sbjct: 74  NPDPGHSIQDIYEQVFGKPWDSGKPDPNPGHPNMSGFAQNA--ERNKKGMSSAVMNGFKP 133

Query: 145 DLVVVYKSLVSVFAVFDRSGNCNYQSISLIVNENRNVQTDFIGGLHRHRPQRCRIGSTFT 204
           + + VYK LV  FA+ DR     + S+      NR         L+ H        +T  
Sbjct: 134 NALPVYKELVQNFAICDRW----FASVPASTQPNR---------LYVH-------SATSH 193

Query: 205 QEPPLEPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTLAYGNLRKLKYLNKFHLFDL 264
                + ++       K I     +AG SFGIY+Q  PSTL Y NLRKLKYL  FH + +
Sbjct: 194 GATSNDKKLLLEGFPQKTIFESLDEAGFSFGIYYQFPPSTLFYRNLRKLKYLTHFHQYGI 253

Query: 265 DFKRHANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQGQMLIKEVYETLRSSPQWIE 324
            FK+   +GKLPNYVV+E ++F+L     +DDHPSHDV +GQ L+KEVYE LRSSPQW E
Sbjct: 254 QFKKDCKEGKLPNYVVVEQRWFDLLSTPANDDHPSHDVSEGQKLVKEVYEALRSSPQWNE 313

Query: 325 TLFIITYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFDRLGIRVSTIMISPWIEKG- 384
            LFIITYDEHGGFYDHVP PV  VP+PDGI+ P  + F F+RLG+RV T  ISPWIE G 
Sbjct: 314 ILFIITYDEHGGFYDHVPTPVDGVPNPDGILGPPPYNFEFNRLGVRVPTFFISPWIEPGT 373

Query: 385 --------------------------------------------------------PKQL 415
                                                                   P+ L
Sbjct: 374 VIHGPNGPYPRSQYEHSSIPATVKTIFKLKDFLSKRDSWAGTFESVITRDSPRQDCPETL 433

BLAST of Csor.00g224620 vs. ExPASy Swiss-Prot
Match: Q9SRQ6 (Non-specific phospholipase C3 OS=Arabidopsis thaliana OX=3702 GN=NPC3 PE=1 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 4.9e-81
Identity = 183/473 (38.69%), Postives = 249/473 (52.64%), Query Frame = 0

Query: 23  SSVKTIIVLIMENCFFDHMLGWMKKLNLKINGVDGTE--SNFLNASDQKLKQFLFKDQAH 82
           S +KTI+VL+ EN  FDHMLGW K+LN +I+GV  +E  SN L+ SD    Q  F  ++ 
Sbjct: 14  SPIKTIVVLVQENRSFDHMLGWFKELNPEIDGVSESEPRSNPLSTSDPNSAQIFFGKESQ 73

Query: 83  YVDPDPVQSFQVIREKILG---SDNTSANPPRMNRFAQKAFSMDNTAAMSAD-VMNEFQP 142
            +DPDP  SFQ I E++ G   SD +    P+MN F Q A ++  T  MS   VM  F P
Sbjct: 74  NIDPDPGHSFQAIYEQVFGKPFSDESPYPDPKMNGFVQNAEAI--TKGMSEKVVMQGFPP 133

Query: 143 DLVVVYKSLVSVFAVFDRSGNCNYQSISLIVNENRNVQTDFIGGLHRHRPQRCRIGSTFT 202
           + + V+K LV  FAV DR     + S+      NR         L+ H        +T  
Sbjct: 134 EKLPVFKELVQEFAVCDRW----FSSLPSSTQPNR---------LYVH-------AATSN 193

Query: 203 QEPPLEPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTLAYGNLRKLKYLNKFHLFDL 262
                +     R    + +     ++G +FGIY+Q+ P+ L Y N+RKLKY++ FH + L
Sbjct: 194 GAFSNDTNTLVRGFPQRTVFESLEESGFTFGIYYQSFPNCLFYRNMRKLKYVDNFHQYHL 253

Query: 263 DFKRHANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQGQMLIKEVYETLRSSPQWIE 322
            FKRH  +GKLPNYVV+EP+YF +     +DDHP +DV +GQ L+KE+YE LR+SPQW E
Sbjct: 254 SFKRHCKEGKLPNYVVIEPRYFKILSAPANDDHPKNDVVEGQNLVKEIYEALRASPQWNE 313

Query: 323 TLFIITYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFDRLGIRVSTIMISPWIEKGP 382
            LF++ YDEHGG+YDHVP PV  VP+PDG+V P+ + F+FDRLG+RV  ++ISPWIE G 
Sbjct: 314 ILFVVVYDEHGGYYDHVPTPVIGVPNPDGLVGPEPYNFKFDRLGVRVPALLISPWIEPGT 373

Query: 383 ----------------KQLPA-----------------------------------PVNI 430
                             +PA                                   PV +
Sbjct: 374 VLHEPNGPEPTSQFEHSSIPATLKKIFNLKSFLTKRDEWAGTLDAVINRTSPRTDCPVTL 433

BLAST of Csor.00g224620 vs. NCBI nr
Match: KAG6590165.1 (Non-specific phospholipase C2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 886 bits (2290), Expect = 0.0
Identity = 435/435 (100.00%), Postives = 435/435 (100.00%), Query Frame = 0

Query: 1   MAAKSTAFFLLLLAFYTPLLHGSSVKTIIVLIMENCFFDHMLGWMKKLNLKINGVDGTES 60
           MAAKSTAFFLLLLAFYTPLLHGSSVKTIIVLIMENCFFDHMLGWMKKLNLKINGVDGTES
Sbjct: 1   MAAKSTAFFLLLLAFYTPLLHGSSVKTIIVLIMENCFFDHMLGWMKKLNLKINGVDGTES 60

Query: 61  NFLNASDQKLKQFLFKDQAHYVDPDPVQSFQVIREKILGSDNTSANPPRMNRFAQKAFSM 120
           NFLNASDQKLKQFLFKDQAHYVDPDPVQSFQVIREKILGSDNTSANPPRMNRFAQKAFSM
Sbjct: 61  NFLNASDQKLKQFLFKDQAHYVDPDPVQSFQVIREKILGSDNTSANPPRMNRFAQKAFSM 120

Query: 121 DNTAAMSADVMNEFQPDLVVVYKSLVSVFAVFDRSGNCNYQSISLIVNENRNVQTDFIGG 180
           DNTAAMSADVMNEFQPDLVVVYKSLVSVFAVFDRSGNCNYQSISLIVNENRNVQTDFIGG
Sbjct: 121 DNTAAMSADVMNEFQPDLVVVYKSLVSVFAVFDRSGNCNYQSISLIVNENRNVQTDFIGG 180

Query: 181 LHRHRPQRCRIGSTFTQEPPLEPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTLAYG 240
           LHRHRPQRCRIGSTFTQEPPLEPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTLAYG
Sbjct: 181 LHRHRPQRCRIGSTFTQEPPLEPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTLAYG 240

Query: 241 NLRKLKYLNKFHLFDLDFKRHANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQGQML 300
           NLRKLKYLNKFHLFDLDFKRHANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQGQML
Sbjct: 241 NLRKLKYLNKFHLFDLDFKRHANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQGQML 300

Query: 301 IKEVYETLRSSPQWIETLFIITYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFDRLG 360
           IKEVYETLRSSPQWIETLFIITYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFDRLG
Sbjct: 301 IKEVYETLRSSPQWIETLFIITYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFDRLG 360

Query: 361 IRVSTIMISPWIEKGPKQLPAPVNIRNSPANKSAMLTEFQQELMQLAIALKGEYILSSYP 420
           IRVSTIMISPWIEKGPKQLPAPVNIRNSPANKSAMLTEFQQELMQLAIALKGEYILSSYP
Sbjct: 361 IRVSTIMISPWIEKGPKQLPAPVNIRNSPANKSAMLTEFQQELMQLAIALKGEYILSSYP 420

Query: 421 EPLGNDMTVKEGRNI 435
           EPLGNDMTVKEGRNI
Sbjct: 421 EPLGNDMTVKEGRNI 435

BLAST of Csor.00g224620 vs. NCBI nr
Match: KAG7023827.1 (Non-specific phospholipase C2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 758 bits (1958), Expect = 7.22e-275
Identity = 368/370 (99.46%), Postives = 369/370 (99.73%), Query Frame = 0

Query: 7   AFFLLLLAFYTPLLHGSSVKTIIVLIMENCFFDHMLGWMKKLNLKINGVDGTESNFLNAS 66
           +FFLLLLAFYTPLLHGSSVKTIIVLIMENCFFDHMLGWMKKLNLKINGVDGTESNFLNAS
Sbjct: 15  SFFLLLLAFYTPLLHGSSVKTIIVLIMENCFFDHMLGWMKKLNLKINGVDGTESNFLNAS 74

Query: 67  DQKLKQFLFKDQAHYVDPDPVQSFQVIREKILGSDNTSANPPRMNRFAQKAFSMDNTAAM 126
           DQKLKQFLFKDQAHYVDPDPVQSFQVIREKILGSDNTSANPPRMNRFAQKAFSMDNTAAM
Sbjct: 75  DQKLKQFLFKDQAHYVDPDPVQSFQVIREKILGSDNTSANPPRMNRFAQKAFSMDNTAAM 134

Query: 127 SADVMNEFQPDLVVVYKSLVSVFAVFDRSGNCNYQSISLIVNENRNVQTDFIGGLHRHRP 186
           SADVMNEFQPDLVVVYKSLVSVFAVFDRSGNCNYQSISLIVNENRNVQTDFIGGLHRHRP
Sbjct: 135 SADVMNEFQPDLVVVYKSLVSVFAVFDRSGNCNYQSISLIVNENRNVQTDFIGGLHRHRP 194

Query: 187 QRCRIGSTFTQEPPLEPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTLAYGNLRKLK 246
           QRCRIGSTFTQEPPLEPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTLAYGNLRKLK
Sbjct: 195 QRCRIGSTFTQEPPLEPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTLAYGNLRKLK 254

Query: 247 YLNKFHLFDLDFKRHANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQGQMLIKEVYE 306
           YLNKFHLFDLDFKRHANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQGQMLIKEVYE
Sbjct: 255 YLNKFHLFDLDFKRHANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQGQMLIKEVYE 314

Query: 307 TLRSSPQWIETLFIITYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFDRLGIRVSTI 366
           TLRSSPQW ETLFIITYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFDRLGIRVSTI
Sbjct: 315 TLRSSPQWNETLFIITYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFDRLGIRVSTI 374

Query: 367 MISPWIEKGP 376
           MISPWIEKGP
Sbjct: 375 MISPWIEKGP 384

BLAST of Csor.00g224620 vs. NCBI nr
Match: XP_022988071.1 (non-specific phospholipase C2-like [Cucurbita maxima])

HSP 1 Score: 553 bits (1426), Expect = 3.93e-192
Identity = 305/493 (61.87%), Postives = 337/493 (68.36%), Query Frame = 0

Query: 1   MAAKSTAFFLLLLAFYTPLLHGSSVKTIIVLIMENCFFDHMLGWMKKLNLKINGVDGTES 60
           MA KSTAFFLLLLAFYTPLLHGS +KTI+VL+MEN  FDHMLGWMKKLN +INGVDG+ES
Sbjct: 10  MATKSTAFFLLLLAFYTPLLHGSPIKTIVVLVMENRSFDHMLGWMKKLNPEINGVDGSES 69

Query: 61  NFLNASDQKLKQFLFKDQAHYVDPDPVQSFQVIREKILGSDNTSANPPRMNRFAQKAFSM 120
           NFLNA+D K KQF FKDQAHYVDPDP  SFQ IRE+I GS++TSANPP MN FAQ+AFSM
Sbjct: 70  NFLNAADPKSKQFFFKDQAHYVDPDPGHSFQAIREQIFGSNDTSANPPPMNGFAQQAFSM 129

Query: 121 DNTAAMSADVMNEFQPDLVVVYKSLVSVFAVFDRSGNCNYQSISLIVNENRNVQTDFIGG 180
           DNT+AMSADVMN FQPD V VYK+LVS FAVFDR     + S+      NR         
Sbjct: 130 DNTSAMSADVMNGFQPDKVAVYKALVSEFAVFDRW----FASVPASTQPNR--------- 189

Query: 181 LHRHRPQRCRIGSTFTQEPPLEPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTLAYG 240
           L+ H       G+T      L      R      I    YDAG SFGIY+QN+PST+ Y 
Sbjct: 190 LYVHSGTSA--GATSNVASLLAKGYPQR-----TIFENVYDAGLSFGIYYQNVPSTMFYR 249

Query: 241 NLRKLKYLNKFHLFDLDFKRHANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQGQML 300
           NLRKLKYLNKFHLF   FK HA+QGKLPNYVVLEP+YF+LPLE G+DDHPSHDVYQGQM 
Sbjct: 250 NLRKLKYLNKFHLFG-QFKLHASQGKLPNYVVLEPRYFDLPLEPGNDDHPSHDVYQGQMF 309

Query: 301 IKEVYETLRSSPQWIETLFIITYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFDRLG 360
           +KEVYETLR+SPQW ETLFIITYDEHGG+YDHVP PV  VPSPDGIV  + FLF FDRLG
Sbjct: 310 VKEVYETLRASPQWNETLFIITYDEHGGYYDHVPTPVN-VPSPDGIVGQEPFLFNFDRLG 369

Query: 361 IRVSTIMISPWIEKG--------------------------------------------- 420
           +RV TIMISPWIEKG                                             
Sbjct: 370 VRVPTIMISPWIEKGIVVHGPNGSPSPTSEYEHSSIPATVKNIFNLSSPFLTKRDEWAGS 429

Query: 421 ---------------PKQLPAPVNIRNSPANKSAMLTEFQQELMQLAIALKGEYILSSYP 433
                          P+QLP PV IR+SPAN+SA LTEFQQELMQLA  LKGE+ILSSY 
Sbjct: 430 FEFILQTLTSPRSDCPEQLPTPVKIRDSPANESAKLTEFQQELMQLAAVLKGEHILSSYS 480

BLAST of Csor.00g224620 vs. NCBI nr
Match: XP_023516593.1 (non-specific phospholipase C2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 546 bits (1407), Expect = 2.20e-189
Identity = 300/494 (60.73%), Postives = 334/494 (67.61%), Query Frame = 0

Query: 1   MAAKSTAFFLLLLAFYTPLLHGSSVKTIIVLIMENCFFDHMLGWMKKLNLKINGVDGTES 60
           MAAKST FFLLLL FYTPLLH S + TI+VL+MEN  FDHMLGWMKKLN +INGVDG+ES
Sbjct: 1   MAAKSTTFFLLLLTFYTPLLHASPINTIVVLVMENRSFDHMLGWMKKLNPQINGVDGSES 60

Query: 61  NFLNASDQKLKQFLFKDQAHYVDPDPVQSFQVIREKILGSDNTSANPPRMNRFAQKAFSM 120
           NFLNA+D K KQF FKDQAHYVDPDP  SFQ IRE++ GSDNTSANPP MN FAQ+AFSM
Sbjct: 61  NFLNATDPKSKQFFFKDQAHYVDPDPGHSFQAIREQVFGSDNTSANPPPMNGFAQQAFSM 120

Query: 121 DNTAAMSADVMNEFQPDLVVVYKSLVSVFAVFDRSGNCNYQSISLIVNENR-NVQTDFIG 180
           DNT+AMSADVMN FQPD V VYK+LVS FAVFDR     + S+      NR  V +    
Sbjct: 121 DNTSAMSADVMNGFQPDKVAVYKALVSEFAVFDRW----FASLPASTQPNRLYVHSGTSH 180

Query: 181 GLHRHRPQRCRIGSTFTQEPPLEPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTLAY 240
           G   +      I S   +  P            + I    +DAG SFGIY+QN+PST+ Y
Sbjct: 181 GATSN------IASLLAKGYP-----------QRTIFENVHDAGLSFGIYYQNIPSTMFY 240

Query: 241 GNLRKLKYLNKFHLFDLDFKRHANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQGQM 300
            NLRKLKYLNKFHLF   FK+ A QGKLPNYVVLEP+YF+LP E G+DDHPSHDVYQGQM
Sbjct: 241 RNLRKLKYLNKFHLFG-RFKQDARQGKLPNYVVLEPRYFDLPREPGNDDHPSHDVYQGQM 300

Query: 301 LIKEVYETLRSSPQWIETLFIITYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFDRL 360
            +KEVYETLR+SPQW ETLFIITYDEHGG+YDHVP PV  VPSPDGIV P+ FLF FDRL
Sbjct: 301 FVKEVYETLRASPQWNETLFIITYDEHGGYYDHVPTPVN-VPSPDGIVGPEPFLFHFDRL 360

Query: 361 GIRVSTIMISPWIEKG-------------------------------------------- 420
           G+RV TIMISPWIEKG                                            
Sbjct: 361 GVRVPTIMISPWIEKGMVVHGPNGSPSPSSEYEHSSIPATVKNIFNLPSPFLTKRDEWAG 420

Query: 421 ----------------PKQLPAPVNIRNSPANKSAMLTEFQQELMQLAIALKGEYILSSY 433
                           P+QLP PV IR+SPAN+SA LTEFQQELMQLA  LKGE+ILSSY
Sbjct: 421 SFESIVQKLTSPRSDCPEQLPTPVKIRDSPANESAKLTEFQQELMQLAAVLKGEHILSSY 471

BLAST of Csor.00g224620 vs. NCBI nr
Match: XP_023516504.1 (non-specific phospholipase C2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 545 bits (1405), Expect = 4.42e-189
Identity = 300/494 (60.73%), Postives = 334/494 (67.61%), Query Frame = 0

Query: 1   MAAKSTAFFLLLLAFYTPLLHGSSVKTIIVLIMENCFFDHMLGWMKKLNLKINGVDGTES 60
           MAAKST FFLLLL FYTPLLH S + TI+VL+MEN  FDHMLGWMKKLN +INGVDG+ES
Sbjct: 1   MAAKSTTFFLLLLTFYTPLLHASPINTIVVLVMENRSFDHMLGWMKKLNPQINGVDGSES 60

Query: 61  NFLNASDQKLKQFLFKDQAHYVDPDPVQSFQVIREKILGSDNTSANPPRMNRFAQKAFSM 120
           NFLNA+D K KQF FKDQAHYVDPDP  SFQ IRE++ GSDNTSANPP MN FAQ+AFSM
Sbjct: 61  NFLNATDPKSKQFFFKDQAHYVDPDPGHSFQAIREQVFGSDNTSANPPPMNGFAQQAFSM 120

Query: 121 DNTAAMSADVMNEFQPDLVVVYKSLVSVFAVFDRSGNCNYQSISLIVNENR-NVQTDFIG 180
           DNT+AMSADVMN FQPD V VYK+LVS FAVFDR     + S+      NR  V +    
Sbjct: 121 DNTSAMSADVMNGFQPDKVAVYKALVSEFAVFDRW----FASLPASTQPNRLYVHSGTSH 180

Query: 181 GLHRHRPQRCRIGSTFTQEPPLEPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTLAY 240
           G   +      I S   +  P            + I    +DAG SFGIY+QN+PST+ Y
Sbjct: 181 GATSN------IASLLAKGYP-----------QRTIFENVHDAGLSFGIYYQNIPSTMFY 240

Query: 241 GNLRKLKYLNKFHLFDLDFKRHANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQGQM 300
            NLRKLKYLNKFHLF   FK+ A QGKLPNYVVLEP+YF+LPLE G+DDHPSHDVYQGQM
Sbjct: 241 RNLRKLKYLNKFHLFG-RFKQDARQGKLPNYVVLEPRYFDLPLEPGNDDHPSHDVYQGQM 300

Query: 301 LIKEVYETLRSSPQWIETLFIITYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFDRL 360
            +KEVYETLR+SPQW ETLFIITYDEHGG+YDHVP PV  VPSPDGIV  + FLF FDRL
Sbjct: 301 FVKEVYETLRASPQWNETLFIITYDEHGGYYDHVPTPVN-VPSPDGIVGQEPFLFNFDRL 360

Query: 361 GIRVSTIMISPWIEKG-------------------------------------------- 420
           G+RV TIMISPWIEKG                                            
Sbjct: 361 GVRVPTIMISPWIEKGIVVHGPNGSPSPSSEYEHSSIPATVKNIFNLPSPFLTKRDEWAG 420

Query: 421 ----------------PKQLPAPVNIRNSPANKSAMLTEFQQELMQLAIALKGEYILSSY 433
                           P+QLP PV IR+SPAN+SA LTEFQQELMQLA  LKGE+ILSSY
Sbjct: 421 SFESIVQKLTSPRSDCPEQLPTPVKIRDSPANESAKLTEFQQELMQLAAVLKGEHILSSY 471

BLAST of Csor.00g224620 vs. ExPASy TrEMBL
Match: A0A6J1JG63 (non-specific phospholipase C2-like OS=Cucurbita maxima OX=3661 GN=LOC111485424 PE=4 SV=1)

HSP 1 Score: 553 bits (1426), Expect = 1.90e-192
Identity = 305/493 (61.87%), Postives = 337/493 (68.36%), Query Frame = 0

Query: 1   MAAKSTAFFLLLLAFYTPLLHGSSVKTIIVLIMENCFFDHMLGWMKKLNLKINGVDGTES 60
           MA KSTAFFLLLLAFYTPLLHGS +KTI+VL+MEN  FDHMLGWMKKLN +INGVDG+ES
Sbjct: 10  MATKSTAFFLLLLAFYTPLLHGSPIKTIVVLVMENRSFDHMLGWMKKLNPEINGVDGSES 69

Query: 61  NFLNASDQKLKQFLFKDQAHYVDPDPVQSFQVIREKILGSDNTSANPPRMNRFAQKAFSM 120
           NFLNA+D K KQF FKDQAHYVDPDP  SFQ IRE+I GS++TSANPP MN FAQ+AFSM
Sbjct: 70  NFLNAADPKSKQFFFKDQAHYVDPDPGHSFQAIREQIFGSNDTSANPPPMNGFAQQAFSM 129

Query: 121 DNTAAMSADVMNEFQPDLVVVYKSLVSVFAVFDRSGNCNYQSISLIVNENRNVQTDFIGG 180
           DNT+AMSADVMN FQPD V VYK+LVS FAVFDR     + S+      NR         
Sbjct: 130 DNTSAMSADVMNGFQPDKVAVYKALVSEFAVFDRW----FASVPASTQPNR--------- 189

Query: 181 LHRHRPQRCRIGSTFTQEPPLEPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTLAYG 240
           L+ H       G+T      L      R      I    YDAG SFGIY+QN+PST+ Y 
Sbjct: 190 LYVHSGTSA--GATSNVASLLAKGYPQR-----TIFENVYDAGLSFGIYYQNVPSTMFYR 249

Query: 241 NLRKLKYLNKFHLFDLDFKRHANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQGQML 300
           NLRKLKYLNKFHLF   FK HA+QGKLPNYVVLEP+YF+LPLE G+DDHPSHDVYQGQM 
Sbjct: 250 NLRKLKYLNKFHLFG-QFKLHASQGKLPNYVVLEPRYFDLPLEPGNDDHPSHDVYQGQMF 309

Query: 301 IKEVYETLRSSPQWIETLFIITYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFDRLG 360
           +KEVYETLR+SPQW ETLFIITYDEHGG+YDHVP PV  VPSPDGIV  + FLF FDRLG
Sbjct: 310 VKEVYETLRASPQWNETLFIITYDEHGGYYDHVPTPVN-VPSPDGIVGQEPFLFNFDRLG 369

Query: 361 IRVSTIMISPWIEKG--------------------------------------------- 420
           +RV TIMISPWIEKG                                             
Sbjct: 370 VRVPTIMISPWIEKGIVVHGPNGSPSPTSEYEHSSIPATVKNIFNLSSPFLTKRDEWAGS 429

Query: 421 ---------------PKQLPAPVNIRNSPANKSAMLTEFQQELMQLAIALKGEYILSSYP 433
                          P+QLP PV IR+SPAN+SA LTEFQQELMQLA  LKGE+ILSSY 
Sbjct: 430 FEFILQTLTSPRSDCPEQLPTPVKIRDSPANESAKLTEFQQELMQLAAVLKGEHILSSYS 480

BLAST of Csor.00g224620 vs. ExPASy TrEMBL
Match: A0A6J1HAN0 (non-specific phospholipase C2 OS=Cucurbita moschata OX=3662 GN=LOC111461642 PE=4 SV=1)

HSP 1 Score: 540 bits (1392), Expect = 2.84e-187
Identity = 301/494 (60.93%), Postives = 337/494 (68.22%), Query Frame = 0

Query: 1   MAAKSTAFFLL-LLAFYTPLLHGSSVKTIIVLIMENCFFDHMLGWMKKLNLKINGVDGTE 60
           MAAKSTAFFLL LLAF+TPLLH S +KTI+VL+MEN  FDHMLGWMKKLN +INGVDG+E
Sbjct: 10  MAAKSTAFFLLFLLAFHTPLLHASPIKTIVVLVMENRSFDHMLGWMKKLNPEINGVDGSE 69

Query: 61  SNFLNASDQKLKQFLFKDQAHYVDPDPVQSFQVIREKILGSDNTSANPPRMNRFAQKAFS 120
           SNFLNA+D K +QF FKDQAHYVDPDP  SFQ IRE+I GS++TSANPP MN FAQ+AFS
Sbjct: 70  SNFLNAADPKSQQFFFKDQAHYVDPDPGHSFQAIREQIFGSNDTSANPPPMNGFAQQAFS 129

Query: 121 MDNTAAMSADVMNEFQPDLVVVYKSLVSVFAVFDRSGNCNYQSISLIVNENRNVQTDFIG 180
           MDNT+AMSADVMN FQPD V VYK+LVS FAVFDR     + S+      NR        
Sbjct: 130 MDNTSAMSADVMNGFQPDKVAVYKALVSEFAVFDRW----FASVPASTQPNR-------- 189

Query: 181 GLHRHRPQRCRIGSTFTQEPPLEPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTLAY 240
            L+ H       G+T      L      R      I    YDAG SFGIY+QN+PST+ Y
Sbjct: 190 -LYVHSGTSA--GATSNVASLLAKGYPQR-----TIFENVYDAGLSFGIYYQNVPSTMFY 249

Query: 241 GNLRKLKYLNKFHLFDLDFKRHANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQGQM 300
            NLRKLKYLNKFHLF   FKR A++GKLPNYVVLEP+YF+LPLE G+DDHPSHDVYQGQM
Sbjct: 250 RNLRKLKYLNKFHLFG-QFKRDASEGKLPNYVVLEPRYFDLPLEPGNDDHPSHDVYQGQM 309

Query: 301 LIKEVYETLRSSPQWIETLFIITYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFDRL 360
            +KEVYETLR+SPQW ETLFIITYDEHGG+YDHVP PV  VPSPDGIV  + FLF FDRL
Sbjct: 310 FVKEVYETLRASPQWNETLFIITYDEHGGYYDHVPTPVN-VPSPDGIVGQEPFLFNFDRL 369

Query: 361 GIRVSTIMISPWIEKG-------------------------------------------- 420
           G+RV TIMISPWIEKG                                            
Sbjct: 370 GVRVPTIMISPWIEKGIVVHGPNGSPSPTSEYEHSSIPATVKNIFNLPSPFLTKRDEWAG 429

Query: 421 ----------------PKQLPAPVNIRNSPANKSAMLTEFQQELMQLAIALKGEYILSSY 433
                           P+QLP PV IR+S AN+SA L+EFQQELMQLA  LKGE+ILSSY
Sbjct: 430 SFDFIVQNLTSPRTDCPEQLPTPVKIRDSLANESAKLSEFQQELMQLAAVLKGEHILSSY 481

BLAST of Csor.00g224620 vs. ExPASy TrEMBL
Match: A0A0A0LXS4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G629050 PE=4 SV=1)

HSP 1 Score: 506 bits (1303), Expect = 6.75e-174
Identity = 273/493 (55.38%), Postives = 315/493 (63.89%), Query Frame = 0

Query: 1   MAAKSTAFFLLLLAFYTPLLHGSSVKTIIVLIMENCFFDHMLGWMKKLNLKINGVDGTES 60
           MA KS  FF       TP LH S + TI+VL+MEN  FDHMLGWMKKLN +INGVDG+ES
Sbjct: 1   MAPKSIPFFFFFFFIITPFLHASPITTIVVLVMENRSFDHMLGWMKKLNPQINGVDGSES 60

Query: 61  NFLNASDQKLKQFLFKDQAHYVDPDPVQSFQVIREKILGSDNTSANPPRMNRFAQKAFSM 120
           N L+ +D   K+F F+DQ+HYVDPDP  SFQ IRE+I GSDNTSANPP MN FAQ+AFSM
Sbjct: 61  NLLSTTDPNSKRFFFQDQSHYVDPDPGHSFQAIREQIFGSDNTSANPPPMNGFAQQAFSM 120

Query: 121 DNTAAMSADVMNEFQPDLVVVYKSLVSVFAVFDRSGNCNYQSISLIVNENRNVQTDFIGG 180
           DNT+AMS DVMN F PD V VYK+LVS FAVFDR     + S+      NR         
Sbjct: 121 DNTSAMSGDVMNGFLPDKVAVYKTLVSEFAVFDRW----FASVPASTQPNR--------- 180

Query: 181 LHRHRPQRCRIGSTFTQEPPLEPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTLAYG 240
           L+ H        S         P +  +    + I     DAG SFGIY+QN+P+TL Y 
Sbjct: 181 LYVHSATSAGATSNI-------PALLAKGYPQRTIFENLDDAGMSFGIYYQNIPATLFYR 240

Query: 241 NLRKLKYLNKFHLFDLDFKRHANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQGQML 300
           NLRKLKY+NKFH + L+FK+ A QGKLPNYVV+E +Y +LPLE  +DDHPSHDVYQGQM 
Sbjct: 241 NLRKLKYVNKFHDYGLNFKKDAKQGKLPNYVVVEQRYIDLPLEPANDDHPSHDVYQGQMF 300

Query: 301 IKEVYETLRSSPQWIETLFIITYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFDRLG 360
           IKEVYETLRSSPQW ETLFIITYDEHGGF+DHVP PVT VPSPDGIV P+ FLF F+RLG
Sbjct: 301 IKEVYETLRSSPQWNETLFIITYDEHGGFFDHVPTPVTGVPSPDGIVGPEPFLFGFNRLG 360

Query: 361 IRVSTIMISPWIEKG--------------------------------------------- 420
           +RV TIMISPWIEKG                                             
Sbjct: 361 VRVPTIMISPWIEKGTVVHSPKGSPFQTSEFEHSSIPATVKKLFNLSSPFLTKRDEWAGS 420

Query: 421 ---------------PKQLPAPVNIRNSPANKSAMLTEFQQELMQLAIALKGEYILSSYP 433
                          P+QLP PV IR +PAN+ A LTEFQQELMQLA  +KG+ I +SYP
Sbjct: 421 FEFIVQTRTEPRTDCPEQLPTPVKIRETPANEKANLTEFQQELMQLAAVMKGDNIFTSYP 473

BLAST of Csor.00g224620 vs. ExPASy TrEMBL
Match: A0A1S3BP25 (non-specific phospholipase C2 OS=Cucumis melo OX=3656 GN=LOC103491977 PE=4 SV=1)

HSP 1 Score: 500 bits (1287), Expect = 1.80e-171
Identity = 270/493 (54.77%), Postives = 314/493 (63.69%), Query Frame = 0

Query: 1   MAAKSTAFFLLLLAFYTPLLHGSSVKTIIVLIMENCFFDHMLGWMKKLNLKINGVDGTES 60
           MA KS +FF     F+ P LH S + TI+VL+MEN  FDHMLGWMKKLN +INGV+G+ES
Sbjct: 1   MAPKSISFFFFFFFFFAPFLHASPINTIVVLVMENRSFDHMLGWMKKLNPQINGVNGSES 60

Query: 61  NFLNASDQKLKQFLFKDQAHYVDPDPVQSFQVIREKILGSDNTSANPPRMNRFAQKAFSM 120
           N L+ +D    +F F+DQ+HYVDPDP  SFQ IRE+I GSDNTSANPP MN FAQ+AFSM
Sbjct: 61  NLLSTTDPNSTRFFFQDQSHYVDPDPGHSFQAIREQIFGSDNTSANPPPMNGFAQQAFSM 120

Query: 121 DNTAAMSADVMNEFQPDLVVVYKSLVSVFAVFDRSGNCNYQSISLIVNENRNVQTDFIGG 180
           DNT+AMS DVMN F PD V VYK+LVS FAVFDR     + S+      NR         
Sbjct: 121 DNTSAMSRDVMNGFLPDKVAVYKTLVSEFAVFDRW----FASVPSSTQPNR--------- 180

Query: 181 LHRHRPQRCRIGSTFTQEPPLEPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTLAYG 240
           L+ H        S         P +  +    + I     DAG SFGIY+QN+P+TL Y 
Sbjct: 181 LYVHSATSAGATSNI-------PALLAKGYPQRTIFENLDDAGMSFGIYYQNIPATLFYR 240

Query: 241 NLRKLKYLNKFHLFDLDFKRHANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQGQML 300
           NLRKLKY+NKFH + L+FK+ A QGKLPNYVV+E +Y +LPLE  +DDHPSHDVYQGQM 
Sbjct: 241 NLRKLKYVNKFHEYGLNFKKDAAQGKLPNYVVVEQRYVDLPLEPANDDHPSHDVYQGQMF 300

Query: 301 IKEVYETLRSSPQWIETLFIITYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFDRLG 360
           IKEVYETLRSSPQW ETLFIITYDEHGGFYDHVP PVT VPSPDGIV P+ FLF FDRLG
Sbjct: 301 IKEVYETLRSSPQWNETLFIITYDEHGGFYDHVPTPVTGVPSPDGIVGPEPFLFGFDRLG 360

Query: 361 IRVSTIMISPWIEKG--------------------------------------------- 420
           +RV TIMISPWIEKG                                             
Sbjct: 361 VRVPTIMISPWIEKGTVVHSPEGSPFQTSEFEHSSIPATVKKLFNLSSPFLTKRDEWAGS 420

Query: 421 ---------------PKQLPAPVNIRNSPANKSAMLTEFQQELMQLAIALKGEYILSSYP 433
                          P+QLP P  IR + AN++A LTEFQQELMQLA  + G+ I +SYP
Sbjct: 421 FEFIVQTRTEPRTDCPEQLPTPEKIRETQANENAKLTEFQQELMQLAAVMNGDDIFTSYP 473

BLAST of Csor.00g224620 vs. ExPASy TrEMBL
Match: A0A5D3DX36 (Non-specific phospholipase C2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold289G001120 PE=4 SV=1)

HSP 1 Score: 496 bits (1276), Expect = 8.06e-170
Identity = 269/493 (54.56%), Postives = 314/493 (63.69%), Query Frame = 0

Query: 1   MAAKSTAFFLLLLAFYTPLLHGSSVKTIIVLIMENCFFDHMLGWMKKLNLKINGVDGTES 60
           MA KS +FF     F+ P LH S + TI+VL+MEN  FDHMLGWMKKLN +INGV+G+ES
Sbjct: 1   MAPKSISFFFFFF-FFAPFLHASPINTIVVLVMENRSFDHMLGWMKKLNPQINGVNGSES 60

Query: 61  NFLNASDQKLKQFLFKDQAHYVDPDPVQSFQVIREKILGSDNTSANPPRMNRFAQKAFSM 120
           N L+ +D    +F F+DQ+HYVDPDP  SFQ IRE+I GSDNTSANPP MN FAQ+AFSM
Sbjct: 61  NLLSTTDPNSTRFFFQDQSHYVDPDPGHSFQAIREQIFGSDNTSANPPPMNGFAQQAFSM 120

Query: 121 DNTAAMSADVMNEFQPDLVVVYKSLVSVFAVFDRSGNCNYQSISLIVNENRNVQTDFIGG 180
           DNT+AMS DVMN F PD V VYK+LVS FAVFDR     + S+      NR         
Sbjct: 121 DNTSAMSRDVMNGFLPDKVAVYKTLVSEFAVFDRW----FASVPSSTQPNR--------- 180

Query: 181 LHRHRPQRCRIGSTFTQEPPLEPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTLAYG 240
           L+ H        S         P +  +    + I     DAG SFGIY+QN+P+TL Y 
Sbjct: 181 LYVHSATSAGATSNI-------PALLAKGYPQRTIFENLDDAGMSFGIYYQNIPATLFYR 240

Query: 241 NLRKLKYLNKFHLFDLDFKRHANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQGQML 300
           NLRKLKY+NKFH + L+FK+ A QGKLPNYVV+E +Y +LPLE  +DDHPSHDVYQGQM 
Sbjct: 241 NLRKLKYVNKFHEYGLNFKKDAAQGKLPNYVVVEQRYVDLPLEPANDDHPSHDVYQGQMF 300

Query: 301 IKEVYETLRSSPQWIETLFIITYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFDRLG 360
           +KEVYETLRSSPQW ETLFIITYDEHGGFYDHVP PVT VPSPDGIV P+ FLF FDRLG
Sbjct: 301 VKEVYETLRSSPQWNETLFIITYDEHGGFYDHVPTPVTGVPSPDGIVGPEPFLFGFDRLG 360

Query: 361 IRVSTIMISPWIEKG--------------------------------------------- 420
           +RV TIMISPWIEKG                                             
Sbjct: 361 VRVPTIMISPWIEKGTVVHSPEGSPFQTSEFEHSSIPATVKKLFNLSSPFLTKRDEWAGS 420

Query: 421 ---------------PKQLPAPVNIRNSPANKSAMLTEFQQELMQLAIALKGEYILSSYP 433
                          P+QLP P  IR + AN++A LTEFQQELMQLA  + G+ I +SYP
Sbjct: 421 FEFIVQTRTEPRTDCPEQLPTPEKIRETQANENAKLTEFQQELMQLAAVMNGDDIFTSYP 472

BLAST of Csor.00g224620 vs. TAIR 10
Match: AT2G26870.1 (non-specific phospholipase C2 )

HSP 1 Score: 404.4 bits (1038), Expect = 1.1e-112
Identity = 232/496 (46.77%), Postives = 290/496 (58.47%), Query Frame = 0

Query: 1   MAAKSTAFFLLL--LAFYTPLLHGSSVKTIIVLIMENCFFDHMLGWMKKLNLKINGVDGT 60
           M+ K+ A   LL     Y  +   S +KTI+V++MEN  FDHMLGWMKKLN +INGVDG+
Sbjct: 1   MSIKAFALIQLLSVTILYNHVHATSPIKTIVVVVMENRSFDHMLGWMKKLNPEINGVDGS 60

Query: 61  ESNFLNASDQKLKQFLFKDQAHYVDPDPVQSFQVIREKILGSDNTSANPPRMNRFAQKAF 120
           ESN ++ SD   ++  F   +HYVDPDP  SFQ IRE++ GS++TS +PP MN F Q+A+
Sbjct: 61  ESNPVSVSDPSSRKIKFGSGSHYVDPDPGHSFQAIREQVFGSNDTSMDPPPMNGFVQQAY 120

Query: 121 SMDNTAAMSADVMNEFQPDLVVVYKSLVSVFAVFDRSGNCNYQSISLIVNENRN-VQTDF 180
           S D +  MSA VMN F+PD V VYKSLVS FAVFDR     + S+      NR  V +  
Sbjct: 121 SEDPSGNMSASVMNGFEPDKVPVYKSLVSEFAVFDRW----FASVPSSTQPNRMFVHSGT 180

Query: 181 IGGLHRHRPQRCRIGSTFTQEPPLEPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTL 240
             G   + P    +   + Q               + I     D   SFGIY+QN+P+ L
Sbjct: 181 SAGATSNNP--ISLAKGYPQ---------------RTIFDNLDDEEFSFGIYYQNIPAVL 240

Query: 241 AYGNLRKLKYLNKFHLFDLDFKRHANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQG 300
            Y +LRKLKY+ KFH +   FK HA QGKLP Y V+E +Y +  LE   DDHPSHDVYQG
Sbjct: 241 FYQSLRKLKYVFKFHSYGNSFKDHAKQGKLPAYTVIEQRYMDTLLEPASDDHPSHDVYQG 300

Query: 301 QMLIKEVYETLRSSPQWIETLFIITYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFD 360
           Q  IKEVYETLR+SPQW ETL IITYDEHGG++DHVP PV +VPSPDGIV P  FLF+F+
Sbjct: 301 QKFIKEVYETLRASPQWNETLLIITYDEHGGYFDHVPTPVRNVPSPDGIVGPDPFLFQFN 360

Query: 361 RLGIRVSTIMISPWIEKG------------------------------------------ 420
           RLGIRV TI +SPWIEKG                                          
Sbjct: 361 RLGIRVPTIAVSPWIEKGTVVHGPNGSPFPSSEYEHSSIPATVKKLFNLSSPFLTKRDEW 420

Query: 421 ------------------PKQLPAPVNIRNSPANKSAMLTEFQQELMQLAIALKGEYILS 434
                             P+ LP PV IR   AN+ A+LTEFQQEL+QLA  LKG+ +L+
Sbjct: 421 AGTFENILQIRKEPRTDCPETLPEPVKIRMGEANEKALLTEFQQELVQLAAVLKGDNMLT 475

BLAST of Csor.00g224620 vs. TAIR 10
Match: AT1G07230.1 (non-specific phospholipase C1 )

HSP 1 Score: 334.0 bits (855), Expect = 1.9e-91
Identity = 201/481 (41.79%), Postives = 265/481 (55.09%), Query Frame = 0

Query: 25  VKTIIVLIMENCFFDHMLGWMKKLNLKINGVDGTESNFLNASDQKLKQFLFKDQAHYVDP 84
           +KTI+V++MEN  FDH+LGW+K    +I+G+ G ESN LN SD   K+    D A +VD 
Sbjct: 38  IKTIVVVVMENRSFDHILGWLKSTRPEIDGLTGKESNPLNVSDPNSKKIFVSDDAVFVDM 97

Query: 85  DPVQSFQVIREKILGSDNTSANPPRMNRFAQKAFSMDNTAAMSADVMNEFQPDLVVVYKS 144
           DP  SFQ IRE+I GS++TS + P+MN FAQ++ SM+    M+ +VM+ F+P+++ VY  
Sbjct: 98  DPGHSFQAIREQIFGSNDTSGD-PKMNGFAQQSESME--PGMAKNVMSGFKPEVLPVYTE 157

Query: 145 LVSVFAVFDRSGNCNYQSISLIVNENR-------------NVQTDFIGGLHRHRPQRCRI 204
           L + F VFDR     + S+      NR             NV+ D + G     PQ    
Sbjct: 158 LANEFGVFDRW----FASVPTSTQPNRFYVHSATSHGCSSNVKKDLVKGF----PQ---- 217

Query: 205 GSTFTQEPPLEPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTLAYGNLRKLKYLNKF 264
                                K I     + G SFGIY+QN+P+T  + +LR+LK+L KF
Sbjct: 218 ---------------------KTIFDSLDENGLSFGIYYQNIPATFFFKSLRRLKHLVKF 277

Query: 265 HLFDLDFKRHANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQGQMLIKEVYETLRSS 324
           H + L FK  A  GKLPNY V+E +YF++ L   +DDHPSHDV  GQ  +KEVYETLRSS
Sbjct: 278 HSYALKFKLDAKLGKLPNYSVVEQRYFDIDLFPANDDHPSHDVAAGQRFVKEVYETLRSS 337

Query: 325 PQWIETLFIITYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFDRLGIRVSTIMISPW 384
           PQW E   +ITYDEHGGFYDHVP PV  VP+PDGI+ P  F F FDRLG+RV T +ISPW
Sbjct: 338 PQWKEMALLITYDEHGGFYDHVPTPVKGVPNPDGIIGPDPFYFGFDRLGVRVPTFLISPW 397

Query: 385 IEKGP----------------KQLPAPV-------------------------NIRNSP- 433
           IEKG                   +PA V                          IR+SP 
Sbjct: 398 IEKGTVIHEPEGPTPHSQFEHSSIPATVKKLFNLKSHFLTKRDAWAGTFEKYFRIRDSPR 457

BLAST of Csor.00g224620 vs. TAIR 10
Match: AT3G48610.1 (non-specific phospholipase C6 )

HSP 1 Score: 313.9 bits (803), Expect = 2.0e-85
Identity = 191/469 (40.72%), Postives = 250/469 (53.30%), Query Frame = 0

Query: 23  SSVKTIIVLIMENCFFDHMLGWMK-KLNLKINGVDGTESNFLNASDQKLKQFLFKDQAHY 82
           S +KT++VL++EN  FDH+LGWMK  +N  INGV G E N +  S Q +    F   A +
Sbjct: 37  SPIKTVVVLVLENRSFDHLLGWMKNSVNPTINGVTGQECNPVPNSTQTI---CFTSDAEF 96

Query: 83  VDPDPVQSFQVIREKILGSDNTSANPPRMNRFAQKAFSMDNTAAMSADVMNEFQPDLVVV 142
           VDPDP  SF+ + +++ GS       P M  F ++A SM     +S  VM  F+P+ V V
Sbjct: 97  VDPDPGHSFEAVEQQVFGSG--PGQIPSMMGFVEQALSMPGN--LSETVMKGFRPEAVPV 156

Query: 143 YKSLVSVFAVFDRSGNCNYQSISLIVNENRNVQTDFIGGLHRHRPQRCRIGSTFTQEPPL 202
           Y  LV  FAVFDR     + SI      NR     F+     H       GST   +  L
Sbjct: 157 YAELVKEFAVFDRW----FSSIPGPTQPNRL----FVYSATSH-------GSTSHVKKQL 216

Query: 203 EPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTLAYGNLRKLKYLNKFHLFDLDFKRH 262
                 + ++  ++HS   D    FGIYFQN+P+TL Y NLR+LKY+   H +DL FK+ 
Sbjct: 217 AQGYP-QKTIFDSLHSNDID----FGIYFQNIPTTLFYRNLRQLKYIFNLHQYDLKFKKD 276

Query: 263 ANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQGQMLIKEVYETLRSSPQWIETLFII 322
           A +GKLP+  V+EP+YF+L     +DDHPSHDV  GQ L+KEVYE LRSSPQW ETL +I
Sbjct: 277 AAKGKLPSLTVIEPRYFDLKGLPANDDHPSHDVANGQKLVKEVYEALRSSPQWNETLLVI 336

Query: 323 TYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFDRLGIRVSTIMISPWIEKG------ 382
           TYDEHGGFYDHV  P   +P+PDG   P    F+FDRLG+RV TIM+SPWI+KG      
Sbjct: 337 TYDEHGGFYDHVKTPYVGIPNPDGNTGPAPGFFKFDRLGVRVPTIMVSPWIQKGTVVSEA 396

Query: 383 -----------------------------------------------------PKQLPAP 432
                                                                P  LP  
Sbjct: 397 KGPTESSEYEHSSIPATIKKLFNLSSNFLTHRDAWAATFEDVVSHLTTPRTDCPMTLPEV 456

BLAST of Csor.00g224620 vs. TAIR 10
Match: AT3G03530.1 (non-specific phospholipase C4 )

HSP 1 Score: 311.6 bits (797), Expect = 9.9e-85
Identity = 186/455 (40.88%), Postives = 246/455 (54.07%), Query Frame = 0

Query: 25  VKTIIVLIMENCFFDHMLGWMKKLNLKINGVDGTE--SNFLNASDQKLKQFLFKDQAHYV 84
           +KTI+VL+ EN  FDH LGW K+LN +I+GV  ++  SN +++SD    + +F DQ+ YV
Sbjct: 14  IKTIVVLVQENRSFDHTLGWFKELNREIDGVTKSDPKSNTVSSSDTNSLRVVFGDQSQYV 73

Query: 85  DPDPVQSFQVIREKILGSDNTSANP------PRMNRFAQKAFSMDNTAAMSADVMNEFQP 144
           +PDP  S Q I E++ G    S  P      P M+ FAQ A    N   MS+ VMN F+P
Sbjct: 74  NPDPGHSIQDIYEQVFGKPWDSGKPDPNPGHPNMSGFAQNA--ERNKKGMSSAVMNGFKP 133

Query: 145 DLVVVYKSLVSVFAVFDRSGNCNYQSISLIVNENRNVQTDFIGGLHRHRPQRCRIGSTFT 204
           + + VYK LV  FA+ DR     + S+      NR         L+ H        +T  
Sbjct: 134 NALPVYKELVQNFAICDRW----FASVPASTQPNR---------LYVH-------SATSH 193

Query: 205 QEPPLEPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTLAYGNLRKLKYLNKFHLFDL 264
                + ++       K I     +AG SFGIY+Q  PSTL Y NLRKLKYL  FH + +
Sbjct: 194 GATSNDKKLLLEGFPQKTIFESLDEAGFSFGIYYQFPPSTLFYRNLRKLKYLTHFHQYGI 253

Query: 265 DFKRHANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQGQMLIKEVYETLRSSPQWIE 324
            FK+   +GKLPNYVV+E ++F+L     +DDHPSHDV +GQ L+KEVYE LRSSPQW E
Sbjct: 254 QFKKDCKEGKLPNYVVVEQRWFDLLSTPANDDHPSHDVSEGQKLVKEVYEALRSSPQWNE 313

Query: 325 TLFIITYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFDRLGIRVSTIMISPWIEKG- 384
            LFIITYDEHGGFYDHVP PV  VP+PDGI+ P  + F F+RLG+RV T  ISPWIE G 
Sbjct: 314 ILFIITYDEHGGFYDHVPTPVDGVPNPDGILGPPPYNFEFNRLGVRVPTFFISPWIEPGT 373

Query: 385 --------------------------------------------------------PKQL 415
                                                                   P+ L
Sbjct: 374 VIHGPNGPYPRSQYEHSSIPATVKTIFKLKDFLSKRDSWAGTFESVITRDSPRQDCPETL 433

BLAST of Csor.00g224620 vs. TAIR 10
Match: AT3G03520.1 (non-specific phospholipase C3 )

HSP 1 Score: 303.1 bits (775), Expect = 3.5e-82
Identity = 183/473 (38.69%), Postives = 249/473 (52.64%), Query Frame = 0

Query: 23  SSVKTIIVLIMENCFFDHMLGWMKKLNLKINGVDGTE--SNFLNASDQKLKQFLFKDQAH 82
           S +KTI+VL+ EN  FDHMLGW K+LN +I+GV  +E  SN L+ SD    Q  F  ++ 
Sbjct: 14  SPIKTIVVLVQENRSFDHMLGWFKELNPEIDGVSESEPRSNPLSTSDPNSAQIFFGKESQ 73

Query: 83  YVDPDPVQSFQVIREKILG---SDNTSANPPRMNRFAQKAFSMDNTAAMSAD-VMNEFQP 142
            +DPDP  SFQ I E++ G   SD +    P+MN F Q A ++  T  MS   VM  F P
Sbjct: 74  NIDPDPGHSFQAIYEQVFGKPFSDESPYPDPKMNGFVQNAEAI--TKGMSEKVVMQGFPP 133

Query: 143 DLVVVYKSLVSVFAVFDRSGNCNYQSISLIVNENRNVQTDFIGGLHRHRPQRCRIGSTFT 202
           + + V+K LV  FAV DR     + S+      NR         L+ H        +T  
Sbjct: 134 EKLPVFKELVQEFAVCDRW----FSSLPSSTQPNR---------LYVH-------AATSN 193

Query: 203 QEPPLEPRVTFRHSLAKAIHSERYDAGTSFGIYFQNLPSTLAYGNLRKLKYLNKFHLFDL 262
                +     R    + +     ++G +FGIY+Q+ P+ L Y N+RKLKY++ FH + L
Sbjct: 194 GAFSNDTNTLVRGFPQRTVFESLEESGFTFGIYYQSFPNCLFYRNMRKLKYVDNFHQYHL 253

Query: 263 DFKRHANQGKLPNYVVLEPQYFNLPLELGHDDHPSHDVYQGQMLIKEVYETLRSSPQWIE 322
            FKRH  +GKLPNYVV+EP+YF +     +DDHP +DV +GQ L+KE+YE LR+SPQW E
Sbjct: 254 SFKRHCKEGKLPNYVVIEPRYFKILSAPANDDHPKNDVVEGQNLVKEIYEALRASPQWNE 313

Query: 323 TLFIITYDEHGGFYDHVPMPVTDVPSPDGIVEPKLFLFRFDRLGIRVSTIMISPWIEKGP 382
            LF++ YDEHGG+YDHVP PV  VP+PDG+V P+ + F+FDRLG+RV  ++ISPWIE G 
Sbjct: 314 ILFVVVYDEHGGYYDHVPTPVIGVPNPDGLVGPEPYNFKFDRLGVRVPALLISPWIEPGT 373

Query: 383 ----------------KQLPA-----------------------------------PVNI 430
                             +PA                                   PV +
Sbjct: 374 VLHEPNGPEPTSQFEHSSIPATLKKIFNLKSFLTKRDEWAGTLDAVINRTSPRTDCPVTL 433

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O810201.6e-11146.77Non-specific phospholipase C2 OS=Arabidopsis thaliana OX=3702 GN=NPC2 PE=2 SV=1[more]
Q8L7Y92.6e-9041.79Non-specific phospholipase C1 OS=Arabidopsis thaliana OX=3702 GN=NPC1 PE=2 SV=1[more]
Q8H9652.8e-8440.72Non-specific phospholipase C6 OS=Arabidopsis thaliana OX=3702 GN=NPC6 PE=2 SV=1[more]
Q9SRQ71.4e-8340.88Non-specific phospholipase C4 OS=Arabidopsis thaliana OX=3702 GN=NPC4 PE=1 SV=1[more]
Q9SRQ64.9e-8138.69Non-specific phospholipase C3 OS=Arabidopsis thaliana OX=3702 GN=NPC3 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6590165.10.0100.00Non-specific phospholipase C2, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7023827.17.22e-27599.46Non-specific phospholipase C2, partial [Cucurbita argyrosperma subsp. argyrosper... [more]
XP_022988071.13.93e-19261.87non-specific phospholipase C2-like [Cucurbita maxima][more]
XP_023516593.12.20e-18960.73non-specific phospholipase C2-like [Cucurbita pepo subsp. pepo][more]
XP_023516504.14.42e-18960.73non-specific phospholipase C2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1JG631.90e-19261.87non-specific phospholipase C2-like OS=Cucurbita maxima OX=3661 GN=LOC111485424 P... [more]
A0A6J1HAN02.84e-18760.93non-specific phospholipase C2 OS=Cucurbita moschata OX=3662 GN=LOC111461642 PE=4... [more]
A0A0A0LXS46.75e-17455.38Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G629050 PE=4 SV=1[more]
A0A1S3BP251.80e-17154.77non-specific phospholipase C2 OS=Cucumis melo OX=3656 GN=LOC103491977 PE=4 SV=1[more]
A0A5D3DX368.06e-17054.56Non-specific phospholipase C2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
Match NameE-valueIdentityDescription
AT2G26870.11.1e-11246.77non-specific phospholipase C2 [more]
AT1G07230.11.9e-9141.79non-specific phospholipase C1 [more]
AT3G48610.12.0e-8540.72non-specific phospholipase C6 [more]
AT3G03530.19.9e-8540.88non-specific phospholipase C4 [more]
AT3G03520.13.5e-8238.69non-specific phospholipase C3 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017850Alkaline-phosphatase-like, core domain superfamilyGENE3D3.40.720.10Alkaline Phosphatase, subunit Acoord: 265..396
e-value: 2.6E-30
score: 108.1
coord: 21..174
e-value: 2.6E-13
score: 52.3
IPR007312PhosphoesterasePFAMPF04185Phosphoesterasecoord: 25..375
e-value: 8.8E-57
score: 192.9
IPR007312PhosphoesterasePANTHERPTHR31956NON-SPECIFIC PHOSPHOLIPASE C4-RELATEDcoord: 376..434
IPR007312PhosphoesterasePANTHERPTHR31956NON-SPECIFIC PHOSPHOLIPASE C4-RELATEDcoord: 6..375
NoneNo IPR availablePANTHERPTHR31956:SF26NON-SPECIFIC PHOSPHOLIPASE C2coord: 376..434
NoneNo IPR availablePANTHERPTHR31956:SF26NON-SPECIFIC PHOSPHOLIPASE C2coord: 6..375

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g224620.m01Csor.00g224620.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016788 hydrolase activity, acting on ester bonds
molecular_function GO:0003824 catalytic activity