Csor.00g223870 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g223870
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionL-ascorbate oxidase homolog
LocationCsor_Chr02: 7171478 .. 7173232 (+)
RNA-Seq ExpressionCsor.00g223870
SyntenyCsor.00g223870
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintronterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTCTGGGTTGTTCTTGTTTGCCCTCCTGCTCTGTCTCTCCGCTGCATCGCTCTGGACGGTCCGCGCCGAAGATCCTTACCTCTTCTTCACTTGGAAGGTCACCTACGGCACCATCTCTCCGCTTGGCGTCCCCCAGCAAGGCATACTCATCAACGGCGAGTTCCCCGGCCCTAACATCAACTCCACCACCAACAACAACGTCGTTGTCAACATCTTCAACCACCTGGACGAGCCCTTCCTCATTCATTGGAGCGGCATCCAACACAGGAAGAATTGCTGGCAAGACGGCCTTCCCGGGACCACCTGCCCCATCCCTGCTGGCACCAACTTCACCTACCATTTCCAAGTCAAGGACCAAATCGGAAGCTTCTTCTACTACCCTTCCACCGCCATGCACCGAGCCGCCGGTGGCTTCGGCGGCCTCCGCATCAACAGCCGTCTACTCATCCCCGTCCCTTATGCCGACCCAGAAGACGACTACACCGTCCTCATTGGCGATTGGTATACCAAGAGCCACACCGCTCTGAAGCAACTCTTGGACAGCGGCCGCACCCTGGCTAGACCCGACGGCATCCTCATCAACGGCAAGAACGCAAAGGGCGATGGCAGTGACGAGCCACTCTTCACCATGAAGCCCGAGAAGACCTACAAGTACAGAATTTGCAATGTGGGGCTTAGGACGTCGCTTAACTTCAGAATCCAAAACCATCCCATGAAGCTTGTTGAGATGGAGGGCTCCCACGTGGTCCAAAACGATTACCAATCCCTCGACGTCCACGTGGGGCAATGCTTCTCTGTTTTGGTCACTGCCAACCAAGAGCCCAGAGACTATTACATGGTGGCATCCACTCGGTTCATGAAGACCATTCACATGAGCAAAGCCATTATTCGATACACCAATGGCAAAGGTCCTGCTTCTTCTGATCTTCCAGAGGCACCCATGGGTTGGGCTTGGTCCCTCAATCAATTCCGCACCTTCCGATGGAACCTCACTGCCAGTGCAGCTAGGCCTAACCCCCAAGGCTCCTTCCGCTATGGTTCCATCAACATTACTCGCACCATCAAGCTCGTCAATTCCTTCGGTAACGTCGACGGTAAGCTACGATATGCCATCAACGGCGTCTCTCATGTCGAAACCGAAACTCCATTGAAGCTTGCGGAATACTTCAAGGTCCCTGAGAAGGTGTTCAAGTACGACATGATTACCGACGAGGGACTACCAGAAGGTTCGACCACCATCACTGTGGCTCCTAATGTTCTCAATGCAACCTACCGCAACTTCGTTGAGATCATTTTTGAGAACCACGAGAAGAGGCTCCAATCATGGCATTTGAATGGCTACTCCTTCTTTGCCGTTGCGTAAGTATCAGTAATACAATTGTTTCGCTCCCCCAATGAGTTGTAGCATATATGGTAATGAATTGTTGTGTTGTTTAATGTGGTTTACAGCATCGAGCCAGGGAAATGGTCTCCAGAAAAGCGAACCAACTACAATCTTCTTGATGCGGTGAGCAGGCACACCATCCAAGTTTTCCCCAAGTCGTGGGCGGCCATTCTTCTGACATTCGACAACTGTGGAATGTGGAACTTGAGGTCTGAGCTGACAGAAAACCGTTACTTGGGACAGCAACTGTATGTCAGTGTCCTGTCCCCAGCACTGTCGCTCCGTGATGAGTACAACATTCCAGACCGCACTTTGCTATGTGGAGTGGTGAAGGGCATGCCATTACCCAAACCATATACCATTTGA

mRNA sequence

ATGGGTTCTGGGTTGTTCTTGTTTGCCCTCCTGCTCTGTCTCTCCGCTGCATCGCTCTGGACGGTCCGCGCCGAAGATCCTTACCTCTTCTTCACTTGGAAGGTCACCTACGGCACCATCTCTCCGCTTGGCGTCCCCCAGCAAGGCATACTCATCAACGGCGAGTTCCCCGGCCCTAACATCAACTCCACCACCAACAACAACGTCGTTGTCAACATCTTCAACCACCTGGACGAGCCCTTCCTCATTCATTGGAGCGGCATCCAACACAGGAAGAATTGCTGGCAAGACGGCCTTCCCGGGACCACCTGCCCCATCCCTGCTGGCACCAACTTCACCTACCATTTCCAAGTCAAGGACCAAATCGGAAGCTTCTTCTACTACCCTTCCACCGCCATGCACCGAGCCGCCGGTGGCTTCGGCGGCCTCCGCATCAACAGCCGTCTACTCATCCCCGTCCCTTATGCCGACCCAGAAGACGACTACACCGTCCTCATTGGCGATTGGTATACCAAGAGCCACACCGCTCTGAAGCAACTCTTGGACAGCGGCCGCACCCTGGCTAGACCCGACGGCATCCTCATCAACGGCAAGAACGCAAAGGGCGATGGCAGTGACGAGCCACTCTTCACCATGAAGCCCGAGAAGACCTACAAGTACAGAATTTGCAATGTGGGGCTTAGGACGTCGCTTAACTTCAGAATCCAAAACCATCCCATGAAGCTTGTTGAGATGGAGGGCTCCCACGTGGTCCAAAACGATTACCAATCCCTCGACGTCCACGTGGGGCAATGCTTCTCTGTTTTGGTCACTGCCAACCAAGAGCCCAGAGACTATTACATGGTGGCATCCACTCGGTTCATGAAGACCATTCACATGAGCAAAGCCATTATTCGATACACCAATGGCAAAGGTCCTGCTTCTTCTGATCTTCCAGAGGCACCCATGGGTTGGGCTTGGTCCCTCAATCAATTCCGCACCTTCCGATGGAACCTCACTGCCAGTGCAGCTAGGCCTAACCCCCAAGGCTCCTTCCGCTATGGTTCCATCAACATTACTCGCACCATCAAGCTCGTCAATTCCTTCGGTAACGTCGACGGTAAGCTACGATATGCCATCAACGGCGTCTCTCATGTCGAAACCGAAACTCCATTGAAGCTTGCGGAATACTTCAAGGTCCCTGAGAAGGTGTTCAAGTACGACATGATTACCGACGAGGGACTACCAGAAGGTTCGACCACCATCACTGTGGCTCCTAATGTTCTCAATGCAACCTACCGCAACTTCGTTGAGATCATTTTTGAGAACCACGAGAAGAGGCTCCAATCATGGCATTTGAATGGCTACTCCTTCTTTGCCGTTGCCATCGAGCCAGGGAAATGGTCTCCAGAAAAGCGAACCAACTACAATCTTCTTGATGCGGTGAGCAGGCACACCATCCAAGTTTTCCCCAAGTCGTGGGCGGCCATTCTTCTGACATTCGACAACTGTGGAATGTGGAACTTGAGGTCTGAGCTGACAGAAAACCGTTACTTGGGACAGCAACTGTATGTCAGTGTCCTGTCCCCAGCACTGTCGCTCCGTGATGAGTACAACATTCCAGACCGCACTTTGCTATGTGGAGTGGTGAAGGGCATGCCATTACCCAAACCATATACCATTTGA

Coding sequence (CDS)

ATGGGTTCTGGGTTGTTCTTGTTTGCCCTCCTGCTCTGTCTCTCCGCTGCATCGCTCTGGACGGTCCGCGCCGAAGATCCTTACCTCTTCTTCACTTGGAAGGTCACCTACGGCACCATCTCTCCGCTTGGCGTCCCCCAGCAAGGCATACTCATCAACGGCGAGTTCCCCGGCCCTAACATCAACTCCACCACCAACAACAACGTCGTTGTCAACATCTTCAACCACCTGGACGAGCCCTTCCTCATTCATTGGAGCGGCATCCAACACAGGAAGAATTGCTGGCAAGACGGCCTTCCCGGGACCACCTGCCCCATCCCTGCTGGCACCAACTTCACCTACCATTTCCAAGTCAAGGACCAAATCGGAAGCTTCTTCTACTACCCTTCCACCGCCATGCACCGAGCCGCCGGTGGCTTCGGCGGCCTCCGCATCAACAGCCGTCTACTCATCCCCGTCCCTTATGCCGACCCAGAAGACGACTACACCGTCCTCATTGGCGATTGGTATACCAAGAGCCACACCGCTCTGAAGCAACTCTTGGACAGCGGCCGCACCCTGGCTAGACCCGACGGCATCCTCATCAACGGCAAGAACGCAAAGGGCGATGGCAGTGACGAGCCACTCTTCACCATGAAGCCCGAGAAGACCTACAAGTACAGAATTTGCAATGTGGGGCTTAGGACGTCGCTTAACTTCAGAATCCAAAACCATCCCATGAAGCTTGTTGAGATGGAGGGCTCCCACGTGGTCCAAAACGATTACCAATCCCTCGACGTCCACGTGGGGCAATGCTTCTCTGTTTTGGTCACTGCCAACCAAGAGCCCAGAGACTATTACATGGTGGCATCCACTCGGTTCATGAAGACCATTCACATGAGCAAAGCCATTATTCGATACACCAATGGCAAAGGTCCTGCTTCTTCTGATCTTCCAGAGGCACCCATGGGTTGGGCTTGGTCCCTCAATCAATTCCGCACCTTCCGATGGAACCTCACTGCCAGTGCAGCTAGGCCTAACCCCCAAGGCTCCTTCCGCTATGGTTCCATCAACATTACTCGCACCATCAAGCTCGTCAATTCCTTCGGTAACGTCGACGGTAAGCTACGATATGCCATCAACGGCGTCTCTCATGTCGAAACCGAAACTCCATTGAAGCTTGCGGAATACTTCAAGGTCCCTGAGAAGGTGTTCAAGTACGACATGATTACCGACGAGGGACTACCAGAAGGTTCGACCACCATCACTGTGGCTCCTAATGTTCTCAATGCAACCTACCGCAACTTCGTTGAGATCATTTTTGAGAACCACGAGAAGAGGCTCCAATCATGGCATTTGAATGGCTACTCCTTCTTTGCCGTTGCCATCGAGCCAGGGAAATGGTCTCCAGAAAAGCGAACCAACTACAATCTTCTTGATGCGGTGAGCAGGCACACCATCCAAGTTTTCCCCAAGTCGTGGGCGGCCATTCTTCTGACATTCGACAACTGTGGAATGTGGAACTTGAGGTCTGAGCTGACAGAAAACCGTTACTTGGGACAGCAACTGTATGTCAGTGTCCTGTCCCCAGCACTGTCGCTCCGTGATGAGTACAACATTCCAGACCGCACTTTGCTATGTGGAGTGGTGAAGGGCATGCCATTACCCAAACCATATACCATTTGA

Protein sequence

MGSGLFLFALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPNINSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDMITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI
Homology
BLAST of Csor.00g223870 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 905.6 bits (2339), Expect = 2.8e-262
Identity = 412/550 (74.91%), Postives = 471/550 (85.64%), Query Frame = 0

Query: 4   GLFLFALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPNINS 63
           G+ L A  L L+AA+   V AEDPY    W VTYGT SPLGVPQQ ILING+FPGPNINS
Sbjct: 3   GVKLLAACLYLAAAATVVVHAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINS 62

Query: 64  TTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIG 123
           T+NNNV++N+FN+LDEPFL+ W+GIQHRKNCWQDG PGT CPI  GTN+TYHFQ KDQIG
Sbjct: 63  TSNNNVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIG 122

Query: 124 SFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDS 183
           S+FYYP+T MHRAAGG+GGLR+NSRLLIPVPYADPEDDYTVLIGDWYTKSHT LK+ LD 
Sbjct: 123 SYFYYPTTGMHRAAGGYGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDG 182

Query: 184 GRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLV 243
           GRT+ RPDGI+INGK+ KGDGSD PLFT+KP KTY+ RICNVG++TS+NFRIQNH MKLV
Sbjct: 183 GRTIGRPDGIVINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMKLV 242

Query: 244 EMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNG 303
           EMEGSHV+QNDY SLDVHVGQCF  +VTANQEP+DYYMVAS+RF+KT+  +  ++RY  G
Sbjct: 243 EMEGSHVLQNDYDSLDVHVGQCFGTIVTANQEPKDYYMVASSRFLKTVITTTGLLRYEGG 302

Query: 304 KGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFG 363
           KGPASS LP  P+GWAWSLNQFR+FRWNLTASAARPNPQGS+ YG INITRTIKLVN+ G
Sbjct: 303 KGPASSQLPAGPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQG 362

Query: 364 NVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDMITDEGLPEGSTTITVAPNVLN 423
            VDGKLR+A+NGVSH E ETPLKLAEYF + +KVFKYD ITD+  PE    I + PNVLN
Sbjct: 363 KVDGKLRFALNGVSHTEPETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEPNVLN 422

Query: 424 ATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVF 483
            T+R FVE++FENHEK +QSWHL+GYSFF+VA+EPG W+PEKR NYNLLDAVSRHT+QV+
Sbjct: 423 ITHRTFVEVVFENHEKSVQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHTVQVY 482

Query: 484 PKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVV 543
           PK WAAILLTFDNCGMWN+RSE TE RYLGQQLY SVLSP  SLRDEYN+P+ +L CG+V
Sbjct: 483 PKCWAAILLTFDNCGMWNVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLV 542

Query: 544 KGMPLP-KPY 553
           K  P P  PY
Sbjct: 543 KNTPKPVNPY 552

BLAST of Csor.00g223870 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 9.9e-236
Identity = 378/557 (67.86%), Postives = 447/557 (80.25%), Query Frame = 0

Query: 1   MGSGLFLF-ALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGP 60
           MGSG   F ALLLCLS      V AEDPYL+F W VTYGTI+PLGVPQQGILING+FPGP
Sbjct: 1   MGSGKVTFVALLLCLSVG----VIAEDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGP 60

Query: 61  NINSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVK 120
            IN T+NNN+VVN+FN+LDEPFL  W+G+QHRKN WQDG PGT CPI  G NFTY FQVK
Sbjct: 61  RINCTSNNNIVVNVFNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVK 120

Query: 121 DQIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQ 180
           DQIGS+ Y+P+TA+HRAAGG+G L ++SR LIPVP+ +P D+Y V +GDWY K H  LK+
Sbjct: 121 DQIGSYSYFPTTALHRAAGGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKK 180

Query: 181 LLDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHP 240
           +LD GRT+ RPDGI+INGK+AK   + EPLFTM+  KTY+YR CN+G+R+S+N R Q HP
Sbjct: 181 ILDGGRTIGRPDGIIINGKSAKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHP 240

Query: 241 MKLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIR 300
           MKLVE+EGSH VQN Y SLD+HVGQC SVLVTA+QEP+DYY+V S+RF+K    S AIIR
Sbjct: 241 MKLVELEGSHTVQNIYDSLDLHVGQCLSVLVTADQEPKDYYLVVSSRFLKQALSSVAIIR 300

Query: 301 YTNGKGPASSDLPEAP----MGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRT 360
           Y NGKGPAS +LP  P     G AWS+NQFR+FRWNLTASAARPNPQGS+ YG INITRT
Sbjct: 301 YANGKGPASPELPTPPPENTEGIAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRT 360

Query: 361 IKLVNSFGNVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDMITDEGLPEGSTTI 420
           IK+ NS   V GKLRY +NG+SH   ETPLKL EYF    K FKYD++ DE  P   + +
Sbjct: 361 IKIFNSMSQVGGKLRYGLNGISHTNGETPLKLVEYFGATNKAFKYDLMADEA-PADPSKL 420

Query: 421 TVAPNVLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAV 480
           T+A NV NATYRNFVEIIFENHEK ++++HL+GYSFFAVA+EPG+WSPEKR NYNL+D +
Sbjct: 421 TIATNVKNATYRNFVEIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGL 480

Query: 481 SRHTIQVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPD 540
           SR+ IQV+P SWAAI+LTFDN GMWNLRSE+ E  YLG+QLY SVLSP+ SLRDEYNIPD
Sbjct: 481 SRNNIQVYPNSWAAIMLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIPD 540

Query: 541 RTLLCGVVKGMPLPKPY 553
              LCG+VKG+ +P PY
Sbjct: 541 NHPLCGIVKGLSMPAPY 552

BLAST of Csor.00g223870 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 1.6e-153
Identity = 269/562 (47.86%), Postives = 367/562 (65.30%), Query Frame = 0

Query: 7   LFALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPNINSTTN 66
           LF +LL +   ++    A DPY F+ ++V+Y T SPLGVPQQ I ING+FPGP IN TTN
Sbjct: 3   LFKILLLVFFVNISFCFAADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTN 62

Query: 67  NNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFF 126
            N+VVN+ N LDE  L+HW+GIQ R+  WQDG+ GT CPIP   N+TY FQVKDQIGSFF
Sbjct: 63  ENLVVNVRNKLDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFF 122

Query: 127 YYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRT 186
           Y+PS    RA+GGFG   +N R +IPVP++ P+ D TV IGDWY ++HTAL++ LD G+ 
Sbjct: 123 YFPSLHFQRASGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKD 182

Query: 187 LARPDGILINGK-------NAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHP 246
           L  PDG+LINGK           DG D    T+ P KTY+ R+ NVG+ TSLNFRIQ H 
Sbjct: 183 LGMPDGVLINGKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHN 242

Query: 247 MKLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQE-PRDYYMVASTRFMKTIHMSK--- 306
           + L E EGS+ VQ +Y SLD+HVGQ +S LVT +Q    DYY+VAS R +      +   
Sbjct: 243 LVLAESEGSYTVQQNYTSLDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTG 302

Query: 307 -AIIRYTNGKGPASSDLPEAP---MGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSIN 366
             I++YTN KG A   LP  P       +S+NQ R+ RWN++AS ARPNPQGSF+YGSIN
Sbjct: 303 VGILKYTNSKGKAKGQLPPGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSIN 362

Query: 367 ITRTIKLVNSFG-NVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDMITDEGLPE 426
           +T    L N     + GK R  +NG+S     TP++LA+  KV + V+K D       P+
Sbjct: 363 VTDVYVLRNMPPVTISGKRRTTLNGISFKNPSTPIRLADKLKVKD-VYKLD------FPK 422

Query: 427 GSTT--ITVAPNVLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTN 486
              T    VA +++N TYR F+E++ +N++ ++QS+H++GY+FF V ++ G+W+   R  
Sbjct: 423 RPLTGPAKVATSIINGTYRGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGT 482

Query: 487 YNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLR 546
           YN  D ++R TIQV+P +W+AIL++ DN G WNLR+E  ++ YLGQ+ YV V++P  + +
Sbjct: 483 YNKWDGIARSTIQVYPGAWSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNK 542

Query: 547 DEYNIPDRTLLCGVVKGMPLPK 551
            E+  PD  L CG +  +  P+
Sbjct: 543 TEFGHPDNVLYCGALSKLQKPQ 557

BLAST of Csor.00g223870 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 7.8e-148
Identity = 260/559 (46.51%), Postives = 372/559 (66.55%), Query Frame = 0

Query: 5   LFLFALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPNINST 64
           +F FAL+   S A        DPY+ + + ++Y T SPLGVPQQ I +NG+FPGP IN+T
Sbjct: 11  VFSFALIFGFSFAG-------DPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINAT 70

Query: 65  TNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGS 124
           TN NV VN+ NHLDEP L+ W G+Q R+N WQDG+ GT CPIP   NFTY FQ+KDQIGS
Sbjct: 71  TNYNVHVNVLNHLDEPLLLTWPGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGS 130

Query: 125 FFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSG 184
           +FY PS    RA+GGFG L IN+R L+P+P+ +P+ +   +IGDWYT++HTAL+++LDSG
Sbjct: 131 YFYSPSLNFQRASGGFGALIINNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRILDSG 190

Query: 185 RTLARPDGILINGK------NAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNH 244
           + L  PDG+LINGK      ++  DG +     + P KTY+ R+ NVG+ TSLNFRIQNH
Sbjct: 191 KELGMPDGVLINGKGPFKYNSSVPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRIQNH 250

Query: 245 PMKLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQE-PRDYYMVASTRFMKTIHMSK-- 304
            + L+E EG +  Q ++   DVHVGQ +S LVT +Q    DYY+VAS RF+      +  
Sbjct: 251 KLLLIETEGRYTSQMNFTDFDVHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQRVT 310

Query: 305 --AIIRYTNGKGPASSDLP--EAPMGWAWS-LNQFRTFRWNLTASAARPNPQGSFRYGSI 364
              I+ Y+N KGPAS  LP     +   WS +NQ R  + N +AS ARPNPQGSF YG I
Sbjct: 311 GVGILHYSNSKGPASGPLPVSATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFHYGQI 370

Query: 365 NITRTIKLVN-SFGNVDGKLRYAINGVSHVETETPLKLAEYFKVP-EKVFKY-DMITDEG 424
           NITRT  L +     ++GKLR  +NG+S V   TP++LA+  KV  + +  + D   DE 
Sbjct: 371 NITRTYILRSLPPTKINGKLRATLNGISFVNPSTPMRLADDHKVKGDYMLDFPDRPLDEK 430

Query: 425 LPEGSTTITVAPNVLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRT 484
           LP  S++I      +NATY+ F+++IF+N++ ++QS+H++GY+F+ VA++ G WS ++ +
Sbjct: 431 LPRLSSSI------INATYKGFIQVIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNS 490

Query: 485 NYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSL 544
           +YN  DAV+R T++V+P +W A+L++ DN G+WN+R E  +  YLGQ+ Y+ +++P  + 
Sbjct: 491 SYNNWDAVARSTVEVYPGAWTAVLISLDNVGVWNIRVENLDRWYLGQETYMRIINPEENG 550

Query: 545 RDEYNIPDRTLLCGVVKGM 547
             E + P+  + CG ++ M
Sbjct: 551 STEMDPPENVMYCGALQAM 556

BLAST of Csor.00g223870 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 1.0e-147
Identity = 264/563 (46.89%), Postives = 373/563 (66.25%), Query Frame = 0

Query: 1   MGSGLFLFALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN 60
           + S L  FALL  +S A+       DP++ + ++V+Y T SPLGVPQQ I +NG+FPGP 
Sbjct: 8   LASFLLCFALLSAVSFAA-------DPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPL 67

Query: 61  INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD 120
           +N+TTN NVVVN+FNHLDEP L+ W GIQ R+N WQDG+ GT CPIP   NFTY FQVKD
Sbjct: 68  LNATTNYNVVVNVFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKD 127

Query: 121 QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQL 180
           QIGSFFY PS    RA+GGFG + IN+R +IP+P+  P+ +   +IGDWYT+ H AL++ 
Sbjct: 128 QIGSFFYSPSLNFQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRA 187

Query: 181 LDSGRTLARPDGILINGK------NAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFR 240
           LDSG+ L  PDG+LINGK      ++  DG D   F ++P KTY+ R+ NVG+ TSLNFR
Sbjct: 188 LDSGKELGMPDGVLINGKGPYKYNSSVPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFR 247

Query: 241 IQNHPMKLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQE-PRDYYMVASTRFMKTIHM 300
           IQNH + LVE EG +  Q ++   DVHVGQ +S LVT +Q+   DYY+VAS RF+     
Sbjct: 248 IQNHSLLLVETEGHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVW 307

Query: 301 SK----AIIRYTNGKGPASSDL--PEAPMGWAWS-LNQFRTFRWNLTASAARPNPQGSFR 360
            +    AI+ Y+N KGP S  L  P+  +   WS ++Q +T R N +AS ARPNPQGSF 
Sbjct: 308 QRVTGVAILHYSNSKGPVSGPLPVPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFH 367

Query: 361 YGSINITRTIKLVNSFGN-VDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDMITD 420
           YG INIT T  L +     ++G LR  +NG+S V   TP++LA+  KV +  +K D    
Sbjct: 368 YGQINITNTYILRSLPPTIINGALRATLNGISFVNPSTPVRLADRNKV-KGAYKLD---- 427

Query: 421 EGLPEG--STTITVAPNVLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSP 480
              P+   +  + +  +++NATY+ F++++F+N++ ++QS+H++GYSFF V ++ G WS 
Sbjct: 428 --FPDRPFNRPLRLDRSMINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSE 487

Query: 481 EKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSP 540
           +K+ +YN  DA+SR TI+V+P  W A+L++ DN G+WN+R E  +  YLG++ Y+ + +P
Sbjct: 488 DKKGSYNNWDAISRSTIEVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNP 547

Query: 541 ALSLRDEYNIPDRTLLCGVVKGM 547
               + E + PD  L CG +K +
Sbjct: 548 EEDGKTEMDPPDNVLYCGALKNL 556

BLAST of Csor.00g223870 vs. NCBI nr
Match: KAG6605821.1 (L-ascorbate oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG6605822.1 L-ascorbate oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1159 bits (2997), Expect = 0.0
Identity = 554/554 (100.00%), Postives = 554/554 (100.00%), Query Frame = 0

Query: 1   MGSGLFLFALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN 60
           MGSGLFLFALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN
Sbjct: 1   MGSGLFLFALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN 60

Query: 61  INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD 120
           INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD
Sbjct: 61  INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD 120

Query: 121 QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQL 180
           QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQL
Sbjct: 121 QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQL 180

Query: 181 LDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPM 240
           LDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPM
Sbjct: 181 LDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPM 240

Query: 241 KLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRY 300
           KLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRY
Sbjct: 241 KLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRY 300

Query: 301 TNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVN 360
           TNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVN
Sbjct: 301 TNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVN 360

Query: 361 SFGNVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDMITDEGLPEGSTTITVAPN 420
           SFGNVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDMITDEGLPEGSTTITVAPN
Sbjct: 361 SFGNVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDMITDEGLPEGSTTITVAPN 420

Query: 421 VLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTI 480
           VLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTI
Sbjct: 421 VLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTI 480

Query: 481 QVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLC 540
           QVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLC
Sbjct: 481 QVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLC 540

Query: 541 GVVKGMPLPKPYTI 554
           GVVKGMPLPKPYTI
Sbjct: 541 GVVKGMPLPKPYTI 554

BLAST of Csor.00g223870 vs. NCBI nr
Match: XP_022957683.1 (L-ascorbate oxidase homolog [Cucurbita moschata] >XP_022958374.1 L-ascorbate oxidase homolog [Cucurbita moschata])

HSP 1 Score: 1153 bits (2983), Expect = 0.0
Identity = 552/554 (99.64%), Postives = 552/554 (99.64%), Query Frame = 0

Query: 1   MGSGLFLFALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN 60
           MGSGLFL ALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN
Sbjct: 1   MGSGLFLSALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN 60

Query: 61  INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD 120
           INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD
Sbjct: 61  INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD 120

Query: 121 QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQL 180
           QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQL
Sbjct: 121 QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQL 180

Query: 181 LDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPM 240
           LDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPM
Sbjct: 181 LDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPM 240

Query: 241 KLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRY 300
           KLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRY
Sbjct: 241 KLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRY 300

Query: 301 TNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVN 360
           TNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVN
Sbjct: 301 TNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVN 360

Query: 361 SFGNVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDMITDEGLPEGSTTITVAPN 420
           SFGNVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYD ITDEGLPEGSTTITVAPN
Sbjct: 361 SFGNVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPN 420

Query: 421 VLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTI 480
           VLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTI
Sbjct: 421 VLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTI 480

Query: 481 QVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLC 540
           QVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLC
Sbjct: 481 QVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLC 540

Query: 541 GVVKGMPLPKPYTI 554
           GVVKGMPLPKPYTI
Sbjct: 541 GVVKGMPLPKPYTI 554

BLAST of Csor.00g223870 vs. NCBI nr
Match: XP_022995355.1 (L-ascorbate oxidase homolog [Cucurbita maxima])

HSP 1 Score: 1151 bits (2978), Expect = 0.0
Identity = 549/554 (99.10%), Postives = 552/554 (99.64%), Query Frame = 0

Query: 1   MGSGLFLFALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN 60
           MGSGLFLFALLLCLS ASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN
Sbjct: 1   MGSGLFLFALLLCLSTASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN 60

Query: 61  INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD 120
           INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD
Sbjct: 61  INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD 120

Query: 121 QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQL 180
           QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQL
Sbjct: 121 QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQL 180

Query: 181 LDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPM 240
           LDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPM
Sbjct: 181 LDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPM 240

Query: 241 KLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRY 300
           KLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRY
Sbjct: 241 KLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRY 300

Query: 301 TNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVN 360
           TNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVN
Sbjct: 301 TNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVN 360

Query: 361 SFGNVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDMITDEGLPEGSTTITVAPN 420
           SFGNVDGKLRYAINGVSHVE ETPLKLAEYF+VPEKVFKYD+ITDEG+PEGSTTITVAPN
Sbjct: 361 SFGNVDGKLRYAINGVSHVEPETPLKLAEYFRVPEKVFKYDVITDEGIPEGSTTITVAPN 420

Query: 421 VLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTI 480
           VLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTI
Sbjct: 421 VLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTI 480

Query: 481 QVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLC 540
           QVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLC
Sbjct: 481 QVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLC 540

Query: 541 GVVKGMPLPKPYTI 554
           GVVKGMPLPKPYTI
Sbjct: 541 GVVKGMPLPKPYTI 554

BLAST of Csor.00g223870 vs. NCBI nr
Match: XP_023534711.1 (L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo] >XP_023534712.1 L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1149 bits (2971), Expect = 0.0
Identity = 546/554 (98.56%), Postives = 551/554 (99.46%), Query Frame = 0

Query: 1   MGSGLFLFALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN 60
           MGSGLFLFALLLCLS ASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN
Sbjct: 1   MGSGLFLFALLLCLSTASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN 60

Query: 61  INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD 120
           INSTTNNNVVVN+FNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIP GTNFTYHFQVKD
Sbjct: 61  INSTTNNNVVVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPVGTNFTYHFQVKD 120

Query: 121 QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQL 180
           QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTAL+QL
Sbjct: 121 QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALRQL 180

Query: 181 LDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPM 240
           LDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPM
Sbjct: 181 LDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPM 240

Query: 241 KLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRY 300
           KLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKA+IRY
Sbjct: 241 KLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAVIRY 300

Query: 301 TNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVN 360
           TNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVN
Sbjct: 301 TNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVN 360

Query: 361 SFGNVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDMITDEGLPEGSTTITVAPN 420
           SFGNVDGKLRYAINGVSHVETETPLKLAEYF+VPEKVFKYD ITDEGLPEGS+TITVAPN
Sbjct: 361 SFGNVDGKLRYAINGVSHVETETPLKLAEYFRVPEKVFKYDTITDEGLPEGSSTITVAPN 420

Query: 421 VLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTI 480
           VLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTI
Sbjct: 421 VLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTI 480

Query: 481 QVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLC 540
           QVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLC
Sbjct: 481 QVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLC 540

Query: 541 GVVKGMPLPKPYTI 554
           GVVKGMPLPKPYTI
Sbjct: 541 GVVKGMPLPKPYTI 554

BLAST of Csor.00g223870 vs. NCBI nr
Match: XP_022995360.1 (L-ascorbate oxidase homolog isoform X1 [Cucurbita maxima])

HSP 1 Score: 1138 bits (2944), Expect = 0.0
Identity = 549/564 (97.34%), Postives = 551/564 (97.70%), Query Frame = 0

Query: 1   MGSGLFLFALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN 60
           MGSGLFLFALLLCLS ASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN
Sbjct: 1   MGSGLFLFALLLCLSTASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN 60

Query: 61  INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD 120
           INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD
Sbjct: 61  INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD 120

Query: 121 QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYT----VLIGDWYTKSHTA 180
           QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYT    VLIGDWYTKSHTA
Sbjct: 121 QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTDDYTVLIGDWYTKSHTA 180

Query: 181 LKQLLDSGRTLARPDGILINGKNAKGDG------SDEPLFTMKPEKTYKYRICNVGLRTS 240
           LKQLLDSGRTLARPDGILINGKNAKGDG      SDEPLFTMKPEKTYKYRICNVGLRTS
Sbjct: 181 LKQLLDSGRTLARPDGILINGKNAKGDGRFEGDGSDEPLFTMKPEKTYKYRICNVGLRTS 240

Query: 241 LNFRIQNHPMKLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKT 300
           LNFRIQNHPMKLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKT
Sbjct: 241 LNFRIQNHPMKLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKT 300

Query: 301 IHMSKAIIRYTNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSI 360
           IHMSKAIIRYTNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSI
Sbjct: 301 IHMSKAIIRYTNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSI 360

Query: 361 NITRTIKLVNSFGNVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDMITDEGLPE 420
           NITRTIKLVNSFGNVDGKLRYAINGVSHVE ETPLKLAEYF+VPEKVFKYD ITDEG+PE
Sbjct: 361 NITRTIKLVNSFGNVDGKLRYAINGVSHVEPETPLKLAEYFRVPEKVFKYDAITDEGIPE 420

Query: 421 GSTTITVAPNVLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYN 480
           GSTTITVAPNVLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYN
Sbjct: 421 GSTTITVAPNVLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYN 480

Query: 481 LLDAVSRHTIQVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDE 540
           LLDAVSRHTIQVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDE
Sbjct: 481 LLDAVSRHTIQVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDE 540

Query: 541 YNIPDRTLLCGVVKGMPLPKPYTI 554
           YNIPDRTLLCGVVKGMPLPKPYTI
Sbjct: 541 YNIPDRTLLCGVVKGMPLPKPYTI 564

BLAST of Csor.00g223870 vs. ExPASy TrEMBL
Match: A0A6J1H0Y2 (L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111459149 PE=3 SV=1)

HSP 1 Score: 1153 bits (2983), Expect = 0.0
Identity = 552/554 (99.64%), Postives = 552/554 (99.64%), Query Frame = 0

Query: 1   MGSGLFLFALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN 60
           MGSGLFL ALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN
Sbjct: 1   MGSGLFLSALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN 60

Query: 61  INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD 120
           INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD
Sbjct: 61  INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD 120

Query: 121 QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQL 180
           QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQL
Sbjct: 121 QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQL 180

Query: 181 LDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPM 240
           LDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPM
Sbjct: 181 LDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPM 240

Query: 241 KLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRY 300
           KLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRY
Sbjct: 241 KLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRY 300

Query: 301 TNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVN 360
           TNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVN
Sbjct: 301 TNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVN 360

Query: 361 SFGNVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDMITDEGLPEGSTTITVAPN 420
           SFGNVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYD ITDEGLPEGSTTITVAPN
Sbjct: 361 SFGNVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPN 420

Query: 421 VLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTI 480
           VLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTI
Sbjct: 421 VLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTI 480

Query: 481 QVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLC 540
           QVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLC
Sbjct: 481 QVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLC 540

Query: 541 GVVKGMPLPKPYTI 554
           GVVKGMPLPKPYTI
Sbjct: 541 GVVKGMPLPKPYTI 554

BLAST of Csor.00g223870 vs. ExPASy TrEMBL
Match: A0A6J1K3U4 (L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111490929 PE=3 SV=1)

HSP 1 Score: 1151 bits (2978), Expect = 0.0
Identity = 549/554 (99.10%), Postives = 552/554 (99.64%), Query Frame = 0

Query: 1   MGSGLFLFALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN 60
           MGSGLFLFALLLCLS ASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN
Sbjct: 1   MGSGLFLFALLLCLSTASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN 60

Query: 61  INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD 120
           INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD
Sbjct: 61  INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD 120

Query: 121 QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQL 180
           QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQL
Sbjct: 121 QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQL 180

Query: 181 LDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPM 240
           LDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPM
Sbjct: 181 LDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPM 240

Query: 241 KLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRY 300
           KLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRY
Sbjct: 241 KLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRY 300

Query: 301 TNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVN 360
           TNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVN
Sbjct: 301 TNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVN 360

Query: 361 SFGNVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDMITDEGLPEGSTTITVAPN 420
           SFGNVDGKLRYAINGVSHVE ETPLKLAEYF+VPEKVFKYD+ITDEG+PEGSTTITVAPN
Sbjct: 361 SFGNVDGKLRYAINGVSHVEPETPLKLAEYFRVPEKVFKYDVITDEGIPEGSTTITVAPN 420

Query: 421 VLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTI 480
           VLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTI
Sbjct: 421 VLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTI 480

Query: 481 QVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLC 540
           QVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLC
Sbjct: 481 QVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLC 540

Query: 541 GVVKGMPLPKPYTI 554
           GVVKGMPLPKPYTI
Sbjct: 541 GVVKGMPLPKPYTI 554

BLAST of Csor.00g223870 vs. ExPASy TrEMBL
Match: A0A6J1K3U8 (L-ascorbate oxidase homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490934 PE=3 SV=1)

HSP 1 Score: 1138 bits (2944), Expect = 0.0
Identity = 549/564 (97.34%), Postives = 551/564 (97.70%), Query Frame = 0

Query: 1   MGSGLFLFALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN 60
           MGSGLFLFALLLCLS ASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN
Sbjct: 1   MGSGLFLFALLLCLSTASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN 60

Query: 61  INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD 120
           INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD
Sbjct: 61  INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD 120

Query: 121 QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYT----VLIGDWYTKSHTA 180
           QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYT    VLIGDWYTKSHTA
Sbjct: 121 QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTDDYTVLIGDWYTKSHTA 180

Query: 181 LKQLLDSGRTLARPDGILINGKNAKGDG------SDEPLFTMKPEKTYKYRICNVGLRTS 240
           LKQLLDSGRTLARPDGILINGKNAKGDG      SDEPLFTMKPEKTYKYRICNVGLRTS
Sbjct: 181 LKQLLDSGRTLARPDGILINGKNAKGDGRFEGDGSDEPLFTMKPEKTYKYRICNVGLRTS 240

Query: 241 LNFRIQNHPMKLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKT 300
           LNFRIQNHPMKLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKT
Sbjct: 241 LNFRIQNHPMKLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKT 300

Query: 301 IHMSKAIIRYTNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSI 360
           IHMSKAIIRYTNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSI
Sbjct: 301 IHMSKAIIRYTNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSI 360

Query: 361 NITRTIKLVNSFGNVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDMITDEGLPE 420
           NITRTIKLVNSFGNVDGKLRYAINGVSHVE ETPLKLAEYF+VPEKVFKYD ITDEG+PE
Sbjct: 361 NITRTIKLVNSFGNVDGKLRYAINGVSHVEPETPLKLAEYFRVPEKVFKYDAITDEGIPE 420

Query: 421 GSTTITVAPNVLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYN 480
           GSTTITVAPNVLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYN
Sbjct: 421 GSTTITVAPNVLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYN 480

Query: 481 LLDAVSRHTIQVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDE 540
           LLDAVSRHTIQVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDE
Sbjct: 481 LLDAVSRHTIQVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDE 540

Query: 541 YNIPDRTLLCGVVKGMPLPKPYTI 554
           YNIPDRTLLCGVVKGMPLPKPYTI
Sbjct: 541 YNIPDRTLLCGVVKGMPLPKPYTI 564

BLAST of Csor.00g223870 vs. ExPASy TrEMBL
Match: A0A6J1K1R2 (L-ascorbate oxidase homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490934 PE=3 SV=1)

HSP 1 Score: 1122 bits (2901), Expect = 0.0
Identity = 540/557 (96.95%), Postives = 545/557 (97.85%), Query Frame = 0

Query: 1   MGSGLFLFALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN 60
           MGSGLFLFALLLCLS ASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN
Sbjct: 1   MGSGLFLFALLLCLSTASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN 60

Query: 61  INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD 120
           INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD
Sbjct: 61  INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD 120

Query: 121 QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHT---AL 180
           QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHT   AL
Sbjct: 121 QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTSHTAL 180

Query: 181 KQLLDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQN 240
           KQLLDSGRTLARPDGILINGKNAKGDG+      ++PEKTYKYRICNVGLRTSLNFRIQN
Sbjct: 181 KQLLDSGRTLARPDGILINGKNAKGDGN------LQPEKTYKYRICNVGLRTSLNFRIQN 240

Query: 241 HPMKLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAI 300
           HPMKLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAI
Sbjct: 241 HPMKLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAI 300

Query: 301 IRYTNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIK 360
           IRYTNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIK
Sbjct: 301 IRYTNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIK 360

Query: 361 LVNSFGNVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDMITDEGLPEGSTTITV 420
           LVNSFGNVDGKLRYAINGVSHVE ETPLKLAEYF+VPEKVFKYD ITDEG+PEGSTTITV
Sbjct: 361 LVNSFGNVDGKLRYAINGVSHVEPETPLKLAEYFRVPEKVFKYDAITDEGIPEGSTTITV 420

Query: 421 APNVLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSR 480
           APNVLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSR
Sbjct: 421 APNVLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSR 480

Query: 481 HTIQVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRT 540
           HTIQVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRT
Sbjct: 481 HTIQVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRT 540

Query: 541 LLCGVVKGMPLPKPYTI 554
           LLCGVVKGMPLPKPYTI
Sbjct: 541 LLCGVVKGMPLPKPYTI 551

BLAST of Csor.00g223870 vs. ExPASy TrEMBL
Match: A0A6J1K1Q7 (L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111490931 PE=3 SV=1)

HSP 1 Score: 1101 bits (2848), Expect = 0.0
Identity = 520/548 (94.89%), Postives = 531/548 (96.90%), Query Frame = 0

Query: 7   LFALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPNINSTTN 66
           +F LLLC+ AASLW V AEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPNINSTTN
Sbjct: 6   IFTLLLCVCAASLWAVHAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPNINSTTN 65

Query: 67  NNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFF 126
           NNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFF
Sbjct: 66  NNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFF 125

Query: 127 YYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRT 186
           YYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHT LKQ LDSGRT
Sbjct: 126 YYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQFLDSGRT 185

Query: 187 LARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEME 246
           LARPDG+LINGKN KGDGSDEPLFTMKPEKTYKYRICNVGL+ SLNFRIQ H MKLVEME
Sbjct: 186 LARPDGVLINGKNGKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQGHSMKLVEME 245

Query: 247 GSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGP 306
           GSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRF+KTIHMSKAIIRYTNGKGP
Sbjct: 246 GSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKTIHMSKAIIRYTNGKGP 305

Query: 307 ASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVD 366
           ASSDLPEAP+GWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNS G VD
Sbjct: 306 ASSDLPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSVGKVD 365

Query: 367 GKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDMITDEGLPEGSTTITVAPNVLNATY 426
           GKLRYAINGVSHV+ ETPLKLAEYFKVPEKVFKYD ITDEG+PEGSTTITVAPNVLNATY
Sbjct: 366 GKLRYAINGVSHVDPETPLKLAEYFKVPEKVFKYDTITDEGIPEGSTTITVAPNVLNATY 425

Query: 427 RNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKS 486
           RNFVEIIFENHEK LQ+WHLNGYSFFAVAIEPGKWSPEKR NYNLLDAVSRHTIQV+PKS
Sbjct: 426 RNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQVYPKS 485

Query: 487 WAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGM 546
           W A+LLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGM
Sbjct: 486 WTAVLLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGM 545

Query: 547 PLPKPYTI 554
           PLPKPYTI
Sbjct: 546 PLPKPYTI 553

BLAST of Csor.00g223870 vs. TAIR 10
Match: AT3G13390.1 (SKU5 similar 11 )

HSP 1 Score: 915.6 bits (2365), Expect = 1.9e-266
Identity = 415/549 (75.59%), Postives = 475/549 (86.52%), Query Frame = 0

Query: 4   GLFLFALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPNINS 63
           G+ L A  L L+AA+   VRAEDPY    W VTYGT+SPLGVPQQ ILING+FPGPN+NS
Sbjct: 3   GVKLLAACLYLAAAATVVVRAEDPYFHHVWNVTYGTVSPLGVPQQVILINGQFPGPNVNS 62

Query: 64  TTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIG 123
           T+NNNV++N+FN+LDEPFL+ W+GIQHRKNCWQDG PGT CPI  GTN+TYHFQ KDQIG
Sbjct: 63  TSNNNVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIG 122

Query: 124 SFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDS 183
           S+FYYPSTAMHR+AGGFGGLR+NSRLLIPVPYADPEDDYTVLIGDWYTKSHT LK+ LDS
Sbjct: 123 SYFYYPSTAMHRSAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDS 182

Query: 184 GRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLV 243
           GRTL RPDGILINGK+ KGDGSD PLFT+KP KTY+ RICNVGL+TSLNFRIQNH +KLV
Sbjct: 183 GRTLGRPDGILINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGLKTSLNFRIQNHKLKLV 242

Query: 244 EMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNG 303
           EMEGSHV+QNDY SLDVHVGQC+  ++TANQE +DYYMVAS+RF+K++  +  ++RY  G
Sbjct: 243 EMEGSHVLQNDYDSLDVHVGQCYGTILTANQEAKDYYMVASSRFLKSVITTTGLLRYEGG 302

Query: 304 KGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFG 363
           KGPASS LP  P+GWAWSLNQFR+FRWNLTASAARPNPQGS+ YG INITRTIKLVN+ G
Sbjct: 303 KGPASSQLPPGPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQG 362

Query: 364 NVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDMITDEGLPEGSTTITVAPNVLN 423
            VDGKLRYA+NGVSH + ETPLKLAEYF V +KVFKYD ITD   PE   +I + PNVLN
Sbjct: 363 KVDGKLRYALNGVSHTDPETPLKLAEYFGVADKVFKYDSITDNPTPEQIKSIKIVPNVLN 422

Query: 424 ATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVF 483
            T+R F+E++FENHEK +QSWHL+GYSFFAVA+EPG W+PEKR NYNLLDAVSRHT+QV+
Sbjct: 423 ITHRTFIEVVFENHEKSVQSWHLDGYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQVY 482

Query: 484 PKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVV 543
           PK WAAILLTFDNCGMWN+RSE +E RYLGQQLY SVLSP  SLRDEYN+P+ +L CG+V
Sbjct: 483 PKCWAAILLTFDNCGMWNVRSENSERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLV 542

Query: 544 KGMPLPKPY 553
           KG P P PY
Sbjct: 543 KGTPKPNPY 551

BLAST of Csor.00g223870 vs. TAIR 10
Match: AT1G55570.1 (SKU5 similar 12 )

HSP 1 Score: 904.0 bits (2335), Expect = 5.7e-263
Identity = 411/552 (74.46%), Postives = 472/552 (85.51%), Query Frame = 0

Query: 1   MGSGLFLFALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN 60
           M  G+ L A+ LC++ A++  V+AEDPY    W VTYGT SPLGVPQQ ILING+FPGPN
Sbjct: 1   MKGGVKLLAVCLCVATATVMMVQAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPN 60

Query: 61  INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD 120
           INST+NNNV+VN+FN+LDEPFLI W+GIQHRKNCWQDG  GT CPIP G NFTYHFQ KD
Sbjct: 61  INSTSNNNVIVNVFNNLDEPFLITWAGIQHRKNCWQDGTAGTMCPIPPGQNFTYHFQPKD 120

Query: 121 QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQL 180
           QIGS+FYYP+TAMHRAAGGFGGLR+NSRLLIPVPYADPEDDYT+LI DWYTKSHT LK+ 
Sbjct: 121 QIGSYFYYPTTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTILINDWYTKSHTQLKKF 180

Query: 181 LDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPM 240
           LDSGRT+ RPDGILINGK+ K DGSD+PLFT+KP KTY+ RICNVGL+ SLNFRIQNH M
Sbjct: 181 LDSGRTIGRPDGILINGKSGKTDGSDKPLFTLKPGKTYRVRICNVGLKASLNFRIQNHKM 240

Query: 241 KLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRY 300
           KLVEMEGSHV+QNDY SLDVHVGQCF V+VTA+QEP+DYYM+ASTRF+K    +  ++RY
Sbjct: 241 KLVEMEGSHVLQNDYDSLDVHVGQCFGVIVTADQEPKDYYMIASTRFLKKPLTTTGLLRY 300

Query: 301 TNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVN 360
             GKGPASS LP AP+GWAWSLNQ+R+FRWNLTASAARPNPQGS+ YG INITRTIKLVN
Sbjct: 301 EGGKGPASSQLPAAPVGWAWSLNQYRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVN 360

Query: 361 SFGNVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDMITDEGLPEGSTTITVAPN 420
           + G VDGKLRYA++GVSH + ETPLKLAEYF V +KVFKYD I+D   P+    I + PN
Sbjct: 361 TQGKVDGKLRYALSGVSHTDPETPLKLAEYFGVADKVFKYDTISDNPNPDQIKNIKIEPN 420

Query: 421 VLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTI 480
           VLN T+R F+E++FENHE+ +QSWHL+GYSFFAVA+EPG W+PEKR NYNLLDAVSRHT+
Sbjct: 421 VLNITHRTFIEVVFENHERSVQSWHLDGYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTV 480

Query: 481 QVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLC 540
           QV+PK WAAILLTFDNCGMWN+RSE  E RYLGQQLY SVLSP  SLRDEYN+P+ +L C
Sbjct: 481 QVYPKCWAAILLTFDNCGMWNIRSENAERRYLGQQLYASVLSPEKSLRDEYNMPETSLQC 540

Query: 541 GVVKGMPLPKPY 553
           G+VKG P   PY
Sbjct: 541 GLVKGKPKVNPY 552

BLAST of Csor.00g223870 vs. TAIR 10
Match: AT1G55560.1 (SKU5 similar 14 )

HSP 1 Score: 873.2 bits (2255), Expect = 1.1e-253
Identity = 403/547 (73.67%), Postives = 469/547 (85.74%), Query Frame = 0

Query: 7   LFALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPNINSTTN 66
           L  +L+CL  +++  V A DPY F TW VTYGT SPLGVPQ+ ILING+FPGPN+NST+N
Sbjct: 5   LLTVLVCL-VSTVAIVNAGDPYFFHTWNVTYGTASPLGVPQKVILINGQFPGPNLNSTSN 64

Query: 67  NNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFF 126
           NNVV+N+FNHLDEPFL+ WSGIQHRKNCWQDG+ GT+CPIPAG NFTYHFQ KDQIGS+F
Sbjct: 65  NNVVINVFNHLDEPFLLTWSGIQHRKNCWQDGVAGTSCPIPAGQNFTYHFQPKDQIGSYF 124

Query: 127 YYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRT 186
           YYP+T++HR AGGFGGLR+NSRLLIPVPYADPEDDYTVL+GDWYT  HTALK  LDSGRT
Sbjct: 125 YYPTTSLHRFAGGFGGLRVNSRLLIPVPYADPEDDYTVLLGDWYTAGHTALKNFLDSGRT 184

Query: 187 LARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEME 246
           L  P+G+LINGK+ K  G +EPLFTMKP KTYKYR+CNVG +++LNFRIQNH MKLVEME
Sbjct: 185 LGLPNGVLINGKSGKVGGKNEPLFTMKPGKTYKYRLCNVGFKSTLNFRIQNHKMKLVEME 244

Query: 247 GSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGP 306
           GSHV+QNDY SLDVHVGQCFSVLVTANQ  +DYYMVASTRF+K    +  +IRY      
Sbjct: 245 GSHVIQNDYDSLDVHVGQCFSVLVTANQAAKDYYMVASTRFLKKELSTVGVIRYEGSNVQ 304

Query: 307 ASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVD 366
           AS++LP+AP+GWAWSLNQFR+FRWNLT++AARPNPQGS+ YG INITR+IKLVNS   VD
Sbjct: 305 ASTELPKAPVGWAWSLNQFRSFRWNLTSNAARPNPQGSYHYGKINITRSIKLVNSKSVVD 364

Query: 367 GKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDMITDEGLPEGSTTITVAPNVLNATY 426
           GK+R+  NGVSHV+TETPLKLAEYF++ EKVFKY++I DE   +  T +TV PNVLN T+
Sbjct: 365 GKVRFGFNGVSHVDTETPLKLAEYFQMSEKVFKYNVIKDEPAAK-ITALTVQPNVLNITF 424

Query: 427 RNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKS 486
           R FVEIIFENHEK +QS+HL+GYSFFAVA EPG+W+PEKR NYNLLDAVSRHT+QV+PKS
Sbjct: 425 RTFVEIIFENHEKTMQSFHLDGYSFFAVASEPGRWTPEKRENYNLLDAVSRHTVQVYPKS 484

Query: 487 WAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGM 546
           W+AILLTFDN GMWN+RSE  E +YLG+QLYVSVLSP  SLRDEYNIP  T LCG+VKG+
Sbjct: 485 WSAILLTFDNAGMWNIRSENLERKYLGEQLYVSVLSPEKSLRDEYNIPLNTNLCGIVKGL 544

Query: 547 PLPKPYT 554
           PLP  Y+
Sbjct: 545 PLPAHYS 549

BLAST of Csor.00g223870 vs. TAIR 10
Match: AT3G13400.1 (SKU5 similar 13 )

HSP 1 Score: 867.5 bits (2240), Expect = 5.9e-252
Identity = 401/548 (73.18%), Postives = 469/548 (85.58%), Query Frame = 0

Query: 7   LFALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPNINSTTN 66
           L  +L+CL A+++  V A DPY ++TW VTYGT +PLG+PQQ ILING+FPGPN+NST+N
Sbjct: 6   LLTVLVCL-ASTVALVSAGDPYFYYTWNVTYGTAAPLGIPQQVILINGQFPGPNLNSTSN 65

Query: 67  NNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFF 126
           NNVV+N+FN+LDEPFL+ WSG+QHRKN WQDG+ GT+CPIPAGTNFTYHFQ KDQIGS+F
Sbjct: 66  NNVVINVFNNLDEPFLLTWSGLQHRKNSWQDGVTGTSCPIPAGTNFTYHFQPKDQIGSYF 125

Query: 127 YYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRT 186
           YYPSTA+HR AGGFGGLR+NSRLLIPVPYADPEDD T+LI DWY KSHTALK  LDSGRT
Sbjct: 126 YYPSTALHRFAGGFGGLRVNSRLLIPVPYADPEDDRTILINDWYAKSHTALKNFLDSGRT 185

Query: 187 LARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEME 246
           L  PDG+LINGK+ K  G++ PLFTMKP KTYKYRICNVG +++LNFRIQ H MKLVEME
Sbjct: 186 LGSPDGVLINGKSGKLGGNNAPLFTMKPGKTYKYRICNVGFKSTLNFRIQGHKMKLVEME 245

Query: 247 GSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGP 306
           GSHV+QNDY SLDVHVGQCF+VLVTA+Q  ++YYMVASTRF+K    +  ++ Y      
Sbjct: 246 GSHVLQNDYDSLDVHVGQCFAVLVTADQVAKNYYMVASTRFLKKEVSTVGVMSYEGSNVQ 305

Query: 307 ASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVD 366
           ASSD+P+AP+GWAWSLNQFR+FRWNLTASAARPNPQGS+ YG INITRTIKL N+   V+
Sbjct: 306 ASSDIPKAPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLANTKNLVN 365

Query: 367 GKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDMITDEGLPEGSTTITVAPNVLNATY 426
           GK+R+  NGVSHV+TETPLKLAEYF + EKVFKY++I DE   +  TT+TV PNVLN T+
Sbjct: 366 GKVRFGFNGVSHVDTETPLKLAEYFGMSEKVFKYNVIKDEPAAK-ITTLTVEPNVLNITF 425

Query: 427 RNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKS 486
           R FVE++FENHEK +QS+HL+GYSFFAVA EPG+W+PEKR NYNLLDAVSRHT+QV+PKS
Sbjct: 426 RTFVEVVFENHEKSMQSFHLDGYSFFAVASEPGRWTPEKRNNYNLLDAVSRHTVQVYPKS 485

Query: 487 WAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGM 546
           W+AILLTFDN GMWN+RSE  E RYLGQQLYVSVLSP  SLRDEYNIP  T LCG+VKG+
Sbjct: 486 WSAILLTFDNAGMWNIRSENWERRYLGQQLYVSVLSPEKSLRDEYNIPLNTNLCGIVKGL 545

Query: 547 PLPKPYTI 555
           PLP PYTI
Sbjct: 546 PLPTPYTI 551

BLAST of Csor.00g223870 vs. TAIR 10
Match: AT1G21860.1 (SKU5 similar 7 )

HSP 1 Score: 632.9 bits (1631), Expect = 2.5e-181
Identity = 302/541 (55.82%), Postives = 385/541 (71.16%), Query Frame = 0

Query: 1   MGSGLFLFALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPN 60
           M +   +  LL  +S A      AEDPY FF W VTYG ISPLGV QQGILING+FPGP+
Sbjct: 6   MNTRAMITTLLFLISLAF-----AEDPYRFFEWHVTYGNISPLGVAQQGILINGKFPGPD 65

Query: 61  INSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKD 120
           I S TN+N+++N+FNHLDEPFL+ W+GI++ KN +QDG+ GT CPIP G N+TY  QVKD
Sbjct: 66  IISITNDNLIINVFNHLDEPFLLSWNGIRNWKNSFQDGVYGTMCPIPPGKNYTYALQVKD 125

Query: 121 QIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQL 180
           QIGSF+Y+PS   H+AAGGFGG+RI+SR LIPVP+  P DDYT+L+GDWY  +H  LK  
Sbjct: 126 QIGSFYYFPSLGFHKAAGGFGGIRISSRALIPVPFPTPADDYTLLVGDWYKTNHKDLKAQ 185

Query: 181 LDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPM 240
           LD+G  L  PDGILING+      S      ++P KTY+ RI NVGL+ SLNFRIQNH M
Sbjct: 186 LDNGGKLPLPDGILINGR------SSGATLNIEPGKTYRLRISNVGLQNSLNFRIQNHTM 245

Query: 241 KLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRY 300
           KLVE+EG + +QN + SLDVHVGQ +SVL+TA+Q  +DYY+V S+RF   I  +  ++ Y
Sbjct: 246 KLVEVEGRYTIQNLFSSLDVHVGQSYSVLITADQPAKDYYVVVSSRFTSKILTTTGVLHY 305

Query: 301 TNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVN 360
           +N   P S  +P+ P+  +WS NQ R  R NLTAS  RPNPQGS+RYG INITRTI+L N
Sbjct: 306 SNSVAPVSGPIPDGPIKLSWSFNQARAIRTNLTASGPRPNPQGSYRYGVINITRTIRLAN 365

Query: 361 SFGNVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDMITDEGLPEGSTTITVAPN 420
           + G+++GK RYA+N  S    +TPLKL +YFK+ + V+K   I+D+     +  I    +
Sbjct: 366 NLGHIEGKQRYAVNSASFYPADTPLKLVDYFKI-DGVYKPGSISDQ---PTNGAIFPTTS 425

Query: 421 VLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTI 480
           V+ A +R FVE+IFEN E  +QSWHL+GYSF+ V +E GKWSP  R  YNL DA+ R TI
Sbjct: 426 VMQADFRAFVEVIFENSEDIVQSWHLDGYSFYVVGMELGKWSPASRKVYNLNDAILRCTI 485

Query: 481 QVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLC 540
           QV+P+SW AI +  DN GMWN+RSE+ E +YLGQQ Y+ V + + SLRDEY IP   LLC
Sbjct: 486 QVYPRSWTAIYIALDNVGMWNMRSEIWERQYLGQQFYMRVYTTSTSLRDEYLIPKNALLC 531

Query: 541 G 542
           G
Sbjct: 546 G 531

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q006242.8e-26274.91L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1[more]
P291629.9e-23667.86L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9SU401.6e-15347.86Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=... [more]
Q9FHN67.8e-14846.51Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=... [more]
Q8VXX51.0e-14746.89Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=... [more]
Match NameE-valueIdentityDescription
KAG6605821.10.0100.00L-ascorbate oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022957683.10.099.64L-ascorbate oxidase homolog [Cucurbita moschata] >XP_022958374.1 L-ascorbate oxi... [more]
XP_022995355.10.099.10L-ascorbate oxidase homolog [Cucurbita maxima][more]
XP_023534711.10.098.56L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo] >XP_023534712.1 L-ascor... [more]
XP_022995360.10.097.34L-ascorbate oxidase homolog isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1H0Y20.099.64L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111459149 PE=3 S... [more]
A0A6J1K3U40.099.10L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111490929 PE=3 SV=... [more]
A0A6J1K3U80.097.34L-ascorbate oxidase homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114909... [more]
A0A6J1K1R20.096.95L-ascorbate oxidase homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1114909... [more]
A0A6J1K1Q70.094.89L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111490931 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT3G13390.11.9e-26675.59SKU5 similar 11 [more]
AT1G55570.15.7e-26374.46SKU5 similar 12 [more]
AT1G55560.11.1e-25373.67SKU5 similar 14 [more]
AT3G13400.15.9e-25273.18SKU5 similar 13 [more]
AT1G21860.12.5e-18155.82SKU5 similar 7 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008972CupredoxinGENE3D2.60.40.420coord: 158..338
e-value: 2.5E-36
score: 127.6
IPR008972CupredoxinGENE3D2.60.40.420coord: 29..149
e-value: 7.9E-33
score: 115.3
IPR008972CupredoxinGENE3D2.60.40.420coord: 348..545
e-value: 1.6E-32
score: 114.8
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 157..339
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 20..174
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 350..540
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 384..521
e-value: 1.4E-23
score: 83.1
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 35..148
e-value: 1.8E-39
score: 134.4
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 160..301
e-value: 1.3E-40
score: 139.2
NoneNo IPR availablePANTHERPTHR11709:SF246L-ASCORBATE OXIDASE-LIKE PROTEINcoord: 7..545
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 7..545
IPR034273Ascorbate oxidase homologue, first cupredoxin domainCDDcd13846CuRO_1_AAO_like_1coord: 29..146
e-value: 7.75552E-73
score: 226.134
IPR034271Ascorbate oxidase homologue, second cupredoxin domainCDDcd13872CuRO_2_AAO_like_1coord: 160..300
e-value: 2.54328E-85
score: 259.257

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g223870.m02Csor.00g223870.m02mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005507 copper ion binding
molecular_function GO:0016491 oxidoreductase activity