Csor.00g223810 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g223810
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionPyruvate kinase family protein
LocationCsor_Chr02: 7199789 .. 7202688 (-)
RNA-Seq ExpressionCsor.00g223810
SyntenyCsor.00g223810
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAACACCGGTATTCTCTGCGCCGTCATGCTTGACACCAAGGTTTCTCTCTACCTTCGTTCTCATAACTCATCTCACTTTACTTGATTTGATCGAACCCTCGATTTCTTGCTTTTCGTTTCTGTATGTCTACCTTTCCCCTTTCTGATCCATATATTATCTGGAATTCGATCTCATCTCTTCCGAACTTTCCATTTCATTTTATCTATTTTCGAATTTTCACCTTTCTTATTTCGTCCTTTGTCGCCTTGTTTTGGTTTTATTCTCGTCTGTCAGATATATTGGGCTTTTACTGGTTTTGATTTCGATGTTTCCAGCGAATCTAATTGGTTTCTTCTCCCTTTTGTATGATAAGTCTGTGTTTCCTTCTGCGAGTTTCCCAAAAAAATGCTTAATTTGTCTGTTACATACTTGGGAATAAGAAGATCAAATAGAATTTAGCGTACTTATGATCTGTGAATTTCACTCAGAAGTTAGGGTTTTTTTTTCCTCTTTCTGTCGTATTAAGCTAAATTACAAATCAGCACTTGTTTTACATATTGCAACACATTCTCAACAAGCCAATTACGGGGAGGGATTTTGTGCTTGCAGGATTCTGATCAGATGTGTTTTAATTATTCATTTATTTTCTACTAGTTGAATGCAGAAGTTGGTGTATTGTTTTTACTGAATGCGACTGATATTTGCCATTGTGCTCTGATTCTTACTAAATGTCCTCTGAAATTGTTCTTCCATTAATCCTCAGTTTTCACCTTCTGTTTAGCCATTATGATGATAGCTTCATTAACTTGGTACTGCTTGTTTATTTTGTCTGGATCTTTGTTACTTTCTAATTGCCTTTAATTTGAACACAATCAAGTTGATATTCTCGTATTTGTATTCCGTTGAGGGTAAATCATATTCTTGAATAGTTAGAGGCAGCACTTGAACTGCGAGTAATAATTTTGAGGTTTATTCCCAGTGATATTCACCATTGTTGCTCCTGTTTAGTTGTCTGAGCTTTCTTCAGAGCCAGGAACAAAGCCTTCCGGATTTCTGTTTCATCTCTTTTATTATTGATTTACAGTACGTAGGAAATTTGTTTTGTGACTTCATCCCACATTGGTTGGGGAGGAGAATGAAGCATTGTTTATAAAGGTGTGAAACCTATCCACATGCAGATGCATTTTAAAAACCTTGAGGGGAAACCCGAAAGGGAATGCCCAAAGAGGACAATATTTGCTAGCGGTAGAGGCTTGGGCCGTTACATCACGCATGCCATTCTTATCTTTATTTTGTTGAAAACAACTATGTTTGAAATTTACAACATACAGTTAATCATGTCTATGGAATCATGAAGATACAAATGCCACTTCCGTTACTGGAAGATGCCCCATCCATAACTTTCACCTTTCATTTTAAAGCAACTGTTTTGCACTAATGAAGCTTTGTCGATGCATGACTTTTCAATGAAAACAGGGCCCGGAGATTCGAACTGGATTTCTGAAAGACGGAAAACCCATCCAACTAACACAAGGACAGGAGATAACTATATCTACCGACTACAGCTTGAAGGGTGATGAGAATATGATATGTATGAGCTACAAAAAGTTAGCTGAGGATGTAAAGCCAGGGAGTGTAATACTTTGTTCAGATGGAACAATCTCATTTTCAGTTCTATCTTGTGATAAAGAATCAGGTTTGGTACGATGTCGCTGTGAGAACTCTGCAGTTCTTGGTGAAAGAAAAAATGTTAACCTGCCTGGAGTTATAGTGGATCTCCCTACCCTAACAGAGAAAGATAAGGAAGATATACTACAATGGGGGGTTCCTAATAACATTGATATGATTGCTCTATCTTTCGTTCGGAAAGGTTCTGATCTTGTGGAGGTCCGGAAGTTGCTTGGAAAGCATGCCAAGAGTATTCTCCTCATGTCTAAGGTACATTCAGTTACATCTGTCGAAATATGTTTCTAATTACTGAGAATTTAGCCTTTTCTGGTGATTCTCAGCACAAATGTTTTGAGATTGCTTCTTTTATGCACCATAAATCTCTTACAAAAAGCAATTTCAGCCCTGTGATCTTTCTGGAATTTGACTCACAGGTTGAGAATCAAGAAGGTGTGGCAAATTTTGACGACATACTAGCCAATTCAGATGCGTTTATGGTGGCACGTGGTGACTTGGGAATGGAAATTCCGATTGAGAAGATATTTCTGGCGCAGAAAGTGATGATATACAAGTGCAACATCCAGGGAAAACCTGTTGTCACAGCTACACAGATGTTGGAATCGATGATTAAATCTCCAAGACCAACTAGAGCCGAAGCCACCGATGTTGCGAATGCTGTTCTCGATGGAACCGATTGCGTTATGCTAAGTGGTGAAACAGCTGCCGGAGCATATCCTGAACTTGCTGTTAGAACAATGGCAAAAATATGTGTTGAAGCTGAAAGCACCCTTAATTATGGAGATGTCTTTAAAAGAATTATGGAACACTCCCCAGTTCCCATGAGCCCATTAGAAAGCCTTGCTTCTTCTGCTGTTAGGACTGCAAACTCTGCCAAGGCCGCTCTTATCCTTGTCCTAACCAGAGGAGGAAGCACAGCAAAATTGGTGGCCAAGTATAGACCAGGAACACCCATCTTGTCCGTGGTCGTCCCCGAGATTAAGACAGATTCATTCGATTGGTCGTGCAGCGATGAAGCTCCAGCAAGGCATAGCTTAATCTTTCGTGGCTTGGTGCCGGTTCTTAGCACAGCATCAGCAAGGTCGTCTCATGCCGAGACAACGGAAGAAGCAATAGAGTTCGCTATTCAGCATGCTAAGTTGAAGGGCCTCTGCAAGAATGGAGATTCTGTCGTGGCGCTTCATCGCGTCGGAACAGCATCTGTGATCAAGATTTTGACTGTGAAGTAG

mRNA sequence

ATGGAAAACACCGGTATTCTCTGCGCCGTCATGCTTGACACCAAGGGCCCGGAGATTCGAACTGGATTTCTGAAAGACGGAAAACCCATCCAACTAACACAAGGACAGGAGATAACTATATCTACCGACTACAGCTTGAAGGGTGATGAGAATATGATATGTATGAGCTACAAAAAGTTAGCTGAGGATGTAAAGCCAGGGAGTGTAATACTTTGTTCAGATGGAACAATCTCATTTTCAGTTCTATCTTGTGATAAAGAATCAGGTTTGGTACGATGTCGCTGTGAGAACTCTGCAGTTCTTGGTGAAAGAAAAAATGTTAACCTGCCTGGAGTTATAGTGGATCTCCCTACCCTAACAGAGAAAGATAAGGAAGATATACTACAATGGGGGGTTCCTAATAACATTGATATGATTGCTCTATCTTTCGTTCGGAAAGGTTCTGATCTTGTGGAGGTCCGGAAGTTGCTTGGAAAGCATGCCAAGAGTATTCTCCTCATGTCTAAGGTTGAGAATCAAGAAGGTGTGGCAAATTTTGACGACATACTAGCCAATTCAGATGCGTTTATGGTGGCACGTGGTGACTTGGGAATGGAAATTCCGATTGAGAAGATATTTCTGGCGCAGAAAGTGATGATATACAAGTGCAACATCCAGGGAAAACCTGTTGTCACAGCTACACAGATGTTGGAATCGATGATTAAATCTCCAAGACCAACTAGAGCCGAAGCCACCGATGTTGCGAATGCTGTTCTCGATGGAACCGATTGCGTTATGCTAAGTGGTGAAACAGCTGCCGGAGCATATCCTGAACTTGCTGTTAGAACAATGGCAAAAATATGTGTTGAAGCTGAAAGCACCCTTAATTATGGAGATGTCTTTAAAAGAATTATGGAACACTCCCCAGTTCCCATGAGCCCATTAGAAAGCCTTGCTTCTTCTGCTGTTAGGACTGCAAACTCTGCCAAGGCCGCTCTTATCCTTGTCCTAACCAGAGGAGGAAGCACAGCAAAATTGGTGGCCAAGTATAGACCAGGAACACCCATCTTGTCCGTGGTCGTCCCCGAGATTAAGACAGATTCATTCGATTGGTCGTGCAGCGATGAAGCTCCAGCAAGGCATAGCTTAATCTTTCGTGGCTTGGTGCCGGTTCTTAGCACAGCATCAGCAAGGTCGTCTCATGCCGAGACAACGGAAGAAGCAATAGAGTTCGCTATTCAGCATGCTAAGTTGAAGGGCCTCTGCAAGAATGGAGATTCTGTCGTGGCGCTTCATCGCGTCGGAACAGCATCTGTGATCAAGATTTTGACTGTGAAGTAG

Coding sequence (CDS)

ATGGAAAACACCGGTATTCTCTGCGCCGTCATGCTTGACACCAAGGGCCCGGAGATTCGAACTGGATTTCTGAAAGACGGAAAACCCATCCAACTAACACAAGGACAGGAGATAACTATATCTACCGACTACAGCTTGAAGGGTGATGAGAATATGATATGTATGAGCTACAAAAAGTTAGCTGAGGATGTAAAGCCAGGGAGTGTAATACTTTGTTCAGATGGAACAATCTCATTTTCAGTTCTATCTTGTGATAAAGAATCAGGTTTGGTACGATGTCGCTGTGAGAACTCTGCAGTTCTTGGTGAAAGAAAAAATGTTAACCTGCCTGGAGTTATAGTGGATCTCCCTACCCTAACAGAGAAAGATAAGGAAGATATACTACAATGGGGGGTTCCTAATAACATTGATATGATTGCTCTATCTTTCGTTCGGAAAGGTTCTGATCTTGTGGAGGTCCGGAAGTTGCTTGGAAAGCATGCCAAGAGTATTCTCCTCATGTCTAAGGTTGAGAATCAAGAAGGTGTGGCAAATTTTGACGACATACTAGCCAATTCAGATGCGTTTATGGTGGCACGTGGTGACTTGGGAATGGAAATTCCGATTGAGAAGATATTTCTGGCGCAGAAAGTGATGATATACAAGTGCAACATCCAGGGAAAACCTGTTGTCACAGCTACACAGATGTTGGAATCGATGATTAAATCTCCAAGACCAACTAGAGCCGAAGCCACCGATGTTGCGAATGCTGTTCTCGATGGAACCGATTGCGTTATGCTAAGTGGTGAAACAGCTGCCGGAGCATATCCTGAACTTGCTGTTAGAACAATGGCAAAAATATGTGTTGAAGCTGAAAGCACCCTTAATTATGGAGATGTCTTTAAAAGAATTATGGAACACTCCCCAGTTCCCATGAGCCCATTAGAAAGCCTTGCTTCTTCTGCTGTTAGGACTGCAAACTCTGCCAAGGCCGCTCTTATCCTTGTCCTAACCAGAGGAGGAAGCACAGCAAAATTGGTGGCCAAGTATAGACCAGGAACACCCATCTTGTCCGTGGTCGTCCCCGAGATTAAGACAGATTCATTCGATTGGTCGTGCAGCGATGAAGCTCCAGCAAGGCATAGCTTAATCTTTCGTGGCTTGGTGCCGGTTCTTAGCACAGCATCAGCAAGGTCGTCTCATGCCGAGACAACGGAAGAAGCAATAGAGTTCGCTATTCAGCATGCTAAGTTGAAGGGCCTCTGCAAGAATGGAGATTCTGTCGTGGCGCTTCATCGCGTCGGAACAGCATCTGTGATCAAGATTTTGACTGTGAAGTAG

Protein sequence

MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKLAEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLTEKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFDDILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEHSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDSVVALHRVGTASVIKILTVK
Homology
BLAST of Csor.00g223810 vs. ExPASy Swiss-Prot
Match: Q42954 (Pyruvate kinase, cytosolic isozyme OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 1.6e-225
Identity = 394/439 (89.75%), Postives = 422/439 (96.13%), Query Frame = 0

Query: 1   MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 60
           ME+TGILCAVMLDTKGPEIRTGFLKD KP+QL QGQEITISTDYS+KGDE+MICMSYKKL
Sbjct: 70  MESTGILCAVMLDTKGPEIRTGFLKDAKPVQLKQGQEITISTDYSIKGDESMICMSYKKL 129

Query: 61  AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 120
           AEDVKP SVILC+DG I+F+VLSCDKE+GL RCRCEN+AVLGERKNVNLPGVIVDLPTLT
Sbjct: 130 AEDVKPQSVILCADGQITFTVLSCDKENGLDRCRCENTAVLGERKNVNLPGVIVDLPTLT 189

Query: 121 EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 180
           +KDK+DIL WGVPN+IDMIALSFVRKGSDLVEVRKLLG+HAK+ILLMSKVENQEGVANFD
Sbjct: 190 DKDKDDILNWGVPNHIDMIALSFVRKGSDLVEVRKLLGEHAKNILLMSKVENQEGVANFD 249

Query: 181 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 240
           DIL NSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT
Sbjct: 250 DILLNSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 309

Query: 241 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 300
           RAEATDVANAVLDGTDCVMLSGETAAGAYP+LAV TMAKIC+EAEST++Y DVFKRIM +
Sbjct: 310 RAEATDVANAVLDGTDCVMLSGETAAGAYPDLAVGTMAKICIEAESTIDYPDVFKRIMSN 369

Query: 301 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 360
           +PVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPG PILSVVVPEIKTD
Sbjct: 370 APVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGMPILSVVVPEIKTD 429

Query: 361 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDS 420
           SFDW+CSDE+PARHSLIFRGLVPVL   SAR+SH E+TEEA++FA+QHAK KGLCK GDS
Sbjct: 430 SFDWTCSDESPARHSLIFRGLVPVLHAGSARASHEESTEEALDFALQHAKTKGLCKQGDS 489

Query: 421 VVALHRVGTASVIKILTVK 440
           VVALHRVGTASVIKI+TVK
Sbjct: 490 VVALHRVGTASVIKIVTVK 508

BLAST of Csor.00g223810 vs. ExPASy Swiss-Prot
Match: O65595 (Probable pyruvate kinase, cytosolic isozyme OS=Arabidopsis thaliana OX=3702 GN=At4g26390 PE=3 SV=1)

HSP 1 Score: 741.5 bits (1913), Expect = 5.5e-213
Identity = 373/439 (84.97%), Postives = 403/439 (91.80%), Query Frame = 0

Query: 1   MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 60
           M NTG+LCAVMLDTKGPEIRTGFLKDGKPIQL QGQEITISTDY LKGDE  ICMSYKKL
Sbjct: 59  MLNTGMLCAVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYDLKGDEKTICMSYKKL 118

Query: 61  AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 120
           A+DV PG VILC+DGTIS  VLSCDKE G VRCRCEN+++LGERKNVNLPGV+VDLPTLT
Sbjct: 119 AQDVNPGMVILCADGTISLKVLSCDKEKGTVRCRCENTSMLGERKNVNLPGVVVDLPTLT 178

Query: 121 EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 180
           EKDK+DIL+WGVPN IDMIALSFVRKGSDLV+VRKLLGKHAK+ILLMSKVENQEGVANFD
Sbjct: 179 EKDKQDILEWGVPNQIDMIALSFVRKGSDLVQVRKLLGKHAKTILLMSKVENQEGVANFD 238

Query: 181 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 240
           DIL NSDAFM+ARGDLGMEIPIEKIFLAQKVMIYKCN  GKPVVTATQMLESMIKSPRPT
Sbjct: 239 DILINSDAFMIARGDLGMEIPIEKIFLAQKVMIYKCNFMGKPVVTATQMLESMIKSPRPT 298

Query: 241 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 300
           RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTL+YGD+FKRIM H
Sbjct: 299 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDIFKRIMLH 358

Query: 301 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 360
           + VPMSP+ESLASSAVRTA S++A L++VLTRGGSTA+LVAKYRPG PILSVVVPEI +D
Sbjct: 359 AAVPMSPMESLASSAVRTATSSRATLMMVLTRGGSTARLVAKYRPGIPILSVVVPEITSD 418

Query: 361 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDS 420
           SFDW+CS+EAPARHSLI+RGLVPVL   SAR+S  E+TEE +EFA ++ K K LCK GDS
Sbjct: 419 SFDWACSNEAPARHSLIYRGLVPVLYAGSARASIDESTEETLEFASEYGKKKQLCKTGDS 478

Query: 421 VVALHRVGTASVIKILTVK 440
           VVAL R G A VIKILTVK
Sbjct: 479 VVALFRTGNAIVIKILTVK 497

BLAST of Csor.00g223810 vs. ExPASy Swiss-Prot
Match: Q42806 (Pyruvate kinase, cytosolic isozyme OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 2.2e-198
Identity = 343/439 (78.13%), Postives = 389/439 (88.61%), Query Frame = 0

Query: 1   MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 60
           M NTGILCAVMLDTKGPEIRTGFLKDGKPIQL +GQE+TI+TDY +KGD  MI MSYKKL
Sbjct: 73  MHNTGILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVTITTDYDIKGDPEMISMSYKKL 132

Query: 61  AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 120
              +KPG+ ILCSDGTI+ +VLSCD ++G VRCRCEN+A LGERKNVNLPGV+VDLPTLT
Sbjct: 133 PVHLKPGNTILCSDGTITLTVLSCDPDAGTVRCRCENTATLGERKNVNLPGVVVDLPTLT 192

Query: 121 EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 180
           EKDKEDIL WGVPN IDMIALSFVRKGSDLV VRK+LG HAK+I LMSKVENQEGV NFD
Sbjct: 193 EKDKEDILGWGVPNKIDMIALSFVRKGSDLVNVRKVLGPHAKNIQLMSKVENQEGVLNFD 252

Query: 181 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 240
           +IL  +DAFMVARGDLGMEIP+EKIFLAQK+MIYKCN+ GKPVVTATQMLESMIKSPRPT
Sbjct: 253 EILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLVGKPVVTATQMLESMIKSPRPT 312

Query: 241 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 300
           RAEATDVANAVLDGTDCVMLSGE+AAGAYPELAV+ MA+IC+EAES+L+YG +FK ++  
Sbjct: 313 RAEATDVANAVLDGTDCVMLSGESAAGAYPELAVKIMARICIEAESSLDYGAIFKEMIRS 372

Query: 301 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 360
           +P+PMSPLESLASSAVRTAN AKA LI+VLTRGGSTAKLVAKYRP  PILSVVVP + TD
Sbjct: 373 TPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGSTAKLVAKYRPAVPILSVVVPVLSTD 432

Query: 361 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDS 420
           SFDW+CSDE PARHSLI+RGL+P+L   SA+++ AE+TE  +E A++ A  + LCK GD+
Sbjct: 433 SFDWTCSDETPARHSLIYRGLIPILGEGSAKATDAESTEVILEAALKSATERALCKPGDA 492

Query: 421 VVALHRVGTASVIKILTVK 440
           VVALHR+G ASVIKI  VK
Sbjct: 493 VVALHRIGAASVIKICIVK 511

BLAST of Csor.00g223810 vs. ExPASy Swiss-Prot
Match: P22200 (Pyruvate kinase, cytosolic isozyme OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 677.6 bits (1747), Expect = 9.7e-194
Identity = 336/439 (76.54%), Postives = 388/439 (88.38%), Query Frame = 0

Query: 1   MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 60
           M+NT ILCAVMLDTKGPEIRTGFL DGKPIQL +GQEIT+STDY++KG+E MI MSYKKL
Sbjct: 72  MQNTQILCAVMLDTKGPEIRTGFLTDGKPIQLKEGQEITVSTDYTIKGNEEMISMSYKKL 131

Query: 61  AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 120
             D+KPG+ ILC+DGTI+ +VLSCD  SG VRCRCEN+A LGERKNVNLPGV+VDLPTLT
Sbjct: 132 VMDLKPGNTILCADGTITLTVLSCDPPSGTVRCRCENTATLGERKNVNLPGVVVDLPTLT 191

Query: 121 EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 180
           EKDKEDIL+WGVPNNIDMIALSFVRKGSDLV VRK+LG HAK I LMSKVENQEGV NFD
Sbjct: 192 EKDKEDILEWGVPNNIDMIALSFVRKGSDLVNVRKVLGPHAKRIQLMSKVENQEGVINFD 251

Query: 181 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 240
           +IL  +D+FMVARGDLGMEIP+EKIFLAQK+MIYKCN+ GK VVTATQMLESMIKSP PT
Sbjct: 252 EILRETDSFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKAVVTATQMLESMIKSPAPT 311

Query: 241 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 300
           RAEATDVANAVLDGTDCVMLSGE+AAGAYPELAV+ M++IC+EAES+L+   +FK ++  
Sbjct: 312 RAEATDVANAVLDGTDCVMLSGESAAGAYPELAVKIMSRICIEAESSLDNEAIFKEMIRC 371

Query: 301 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 360
           +P+PMSPLESLASSAVRTAN A+A LI+VLTRGGSTAKLVAKYRP  PILSVVVP + TD
Sbjct: 372 TPLPMSPLESLASSAVRTANKARAKLIVVLTRGGSTAKLVAKYRPAVPILSVVVPVLTTD 431

Query: 361 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDS 420
           SFDWS SDE PARHSL++RGL+P+L   SA+++ +E+TE  +E A++ A  +GLCK GD+
Sbjct: 432 SFDWSISDETPARHSLVYRGLIPLLGEGSAKATDSESTEVILEAALKSAVTRGLCKPGDA 491

Query: 421 VVALHRVGTASVIKILTVK 440
           VVALHR+G+ASVIKI  VK
Sbjct: 492 VVALHRIGSASVIKICVVK 510

BLAST of Csor.00g223810 vs. ExPASy Swiss-Prot
Match: O44006 (Pyruvate kinase OS=Eimeria tenella OX=5802 GN=PYK PE=2 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 2.1e-108
Identity = 213/443 (48.08%), Postives = 291/443 (65.69%), Query Frame = 0

Query: 2   ENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKLA 61
           +  G   A++LDTKGPEIRTG L+  KPI+L  G  + I TDYS  G+++ I  SY+KL 
Sbjct: 110 QRPGKRVALLLDTKGPEIRTGMLEGDKPIELHAGDMLKIVTDYSFVGNKSCIACSYEKLP 169

Query: 62  EDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLTE 121
             VKPG+ IL +DG++S  V+ C K+   V  R  N A++G +KN+NLPGV VDLP + E
Sbjct: 170 SSVKPGNTILIADGSLSVEVVECGKD--YVMTRVMNPAIIGNKKNMNLPGVKVDLPVIGE 229

Query: 122 KDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFDD 181
           KDK DIL +G+P   + IA SFV+   D+  +R +LG   ++I ++ K+EN EG+ NFD+
Sbjct: 230 KDKNDILNFGIPMGCNFIAASFVQSADDVRYIRSILGTKGRNIKIIPKIENVEGLLNFDE 289

Query: 182 ILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTR 241
           IL  +D  M+ARGDLGMEIP EK+FLAQK+MI KCN+ GKPV+TATQMLESM K+PRPTR
Sbjct: 290 ILQEADGIMIARGDLGMEIPPEKVFLAQKMMISKCNVAGKPVITATQMLESMTKNPRPTR 349

Query: 242 AEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEHS 301
           AEA DVANAVLDGTDCVMLSGETA G++P  AV  M+++C EAE  ++Y  VF+   + +
Sbjct: 350 AEAADVANAVLDGTDCVMLSGETANGSFPVQAVTVMSRVCFEAEGCIDYQQVFRATCQAT 409

Query: 302 PVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDS 361
             P+   E++A +AV TA S  A+LIL LT  G TA+L+AKYRP  PIL++         
Sbjct: 410 MTPIDTQEAVARAAVETAQSINASLILALTETGRTARLIAKYRPMQPILAL--------- 469

Query: 362 FDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDSV 421
              S S+E   +   + RG+   L          + T++ I  A+  AK   L   GDS+
Sbjct: 470 ---SASEET-IKQLQVIRGVTTFLVPT------FQGTDQLIRNALSAAKELQLVSEGDSI 529

Query: 422 VALHRV-----GTASVIKILTVK 440
           VA+H +     G ++++K+L V+
Sbjct: 530 VAVHGIKEEVAGWSNLLKVLVVE 531

BLAST of Csor.00g223810 vs. NCBI nr
Match: KAG6605827.1 (hypothetical protein SDJN03_03144, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 850 bits (2197), Expect = 9.29e-311
Identity = 439/439 (100.00%), Postives = 439/439 (100.00%), Query Frame = 0

Query: 1   MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 60
           MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL
Sbjct: 1   MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 60

Query: 61  AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 120
           AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT
Sbjct: 61  AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 120

Query: 121 EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 180
           EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD
Sbjct: 121 EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 180

Query: 181 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 240
           DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT
Sbjct: 181 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 240

Query: 241 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 300
           RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH
Sbjct: 241 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 300

Query: 301 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 360
           SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD
Sbjct: 301 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 360

Query: 361 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDS 420
           SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDS
Sbjct: 361 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDS 420

Query: 421 VVALHRVGTASVIKILTVK 439
           VVALHRVGTASVIKILTVK
Sbjct: 421 VVALHRVGTASVIKILTVK 439

BLAST of Csor.00g223810 vs. NCBI nr
Match: XP_022958623.1 (pyruvate kinase, cytosolic isozyme [Cucurbita moschata] >KAG7035785.1 hypothetical protein SDJN02_02583 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 850 bits (2197), Expect = 9.33e-310
Identity = 439/439 (100.00%), Postives = 439/439 (100.00%), Query Frame = 0

Query: 1   MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 60
           MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL
Sbjct: 62  MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 121

Query: 61  AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 120
           AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT
Sbjct: 122 AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 181

Query: 121 EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 180
           EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD
Sbjct: 182 EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 241

Query: 181 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 240
           DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT
Sbjct: 242 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 301

Query: 241 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 300
           RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH
Sbjct: 302 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 361

Query: 301 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 360
           SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD
Sbjct: 362 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 421

Query: 361 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDS 420
           SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDS
Sbjct: 422 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDS 481

Query: 421 VVALHRVGTASVIKILTVK 439
           VVALHRVGTASVIKILTVK
Sbjct: 482 VVALHRVGTASVIKILTVK 500

BLAST of Csor.00g223810 vs. NCBI nr
Match: XP_022996430.1 (pyruvate kinase, cytosolic isozyme [Cucurbita maxima])

HSP 1 Score: 848 bits (2191), Expect = 7.65e-309
Identity = 437/439 (99.54%), Postives = 439/439 (100.00%), Query Frame = 0

Query: 1   MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 60
           MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL
Sbjct: 62  MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 121

Query: 61  AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 120
           AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT
Sbjct: 122 AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 181

Query: 121 EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 180
           EKDKEDIL+WGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD
Sbjct: 182 EKDKEDILEWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 241

Query: 181 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 240
           DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT
Sbjct: 242 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 301

Query: 241 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 300
           RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH
Sbjct: 302 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 361

Query: 301 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 360
           SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD
Sbjct: 362 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 421

Query: 361 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDS 420
           SFDWSCSDEAPARHSLIFRGLVPVLSTASA+SSHAETTEEAIEFAIQHAKLKGLCKNGDS
Sbjct: 422 SFDWSCSDEAPARHSLIFRGLVPVLSTASAKSSHAETTEEAIEFAIQHAKLKGLCKNGDS 481

Query: 421 VVALHRVGTASVIKILTVK 439
           VVALHRVGTASVIKILTVK
Sbjct: 482 VVALHRVGTASVIKILTVK 500

BLAST of Csor.00g223810 vs. NCBI nr
Match: XP_023533624.1 (pyruvate kinase, cytosolic isozyme [Cucurbita pepo subsp. pepo])

HSP 1 Score: 848 bits (2190), Expect = 1.09e-308
Identity = 438/439 (99.77%), Postives = 439/439 (100.00%), Query Frame = 0

Query: 1   MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 60
           MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL
Sbjct: 62  MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 121

Query: 61  AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 120
           AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT
Sbjct: 122 AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 181

Query: 121 EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 180
           EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD
Sbjct: 182 EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 241

Query: 181 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 240
           DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT
Sbjct: 242 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 301

Query: 241 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 300
           RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH
Sbjct: 302 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 361

Query: 301 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 360
           SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD
Sbjct: 362 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 421

Query: 361 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDS 420
           SFDWSCSDEAPARHSLIFRGLVPVLSTASARSS+AETTEEAIEFAIQHAKLKGLCKNGDS
Sbjct: 422 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSNAETTEEAIEFAIQHAKLKGLCKNGDS 481

Query: 421 VVALHRVGTASVIKILTVK 439
           VVALHRVGTASVIKILTVK
Sbjct: 482 VVALHRVGTASVIKILTVK 500

BLAST of Csor.00g223810 vs. NCBI nr
Match: XP_008449903.1 (PREDICTED: pyruvate kinase, cytosolic isozyme [Cucumis melo] >KAA0040121.1 pyruvate kinase, cytosolic isozyme [Cucumis melo var. makuwa] >TYK21743.1 pyruvate kinase, cytosolic isozyme [Cucumis melo var. makuwa])

HSP 1 Score: 837 bits (2161), Expect = 2.85e-304
Identity = 432/439 (98.41%), Postives = 435/439 (99.09%), Query Frame = 0

Query: 1   MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 60
           MENTGILCAVMLDTKGPEIRTGFLKDGKPIQL QGQEITISTDYSLKGDENMICMSYKKL
Sbjct: 62  MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYSLKGDENMICMSYKKL 121

Query: 61  AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 120
           AEDVKPGSVILCSDGTISFSVLSCDK+ GLVRCRCENSAVLGERKNVNLPGVIVDLPTLT
Sbjct: 122 AEDVKPGSVILCSDGTISFSVLSCDKKLGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 181

Query: 121 EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 180
           EKDKEDIL+WGVPN IDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD
Sbjct: 182 EKDKEDILEWGVPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 241

Query: 181 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 240
           DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT
Sbjct: 242 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 301

Query: 241 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 300
           RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTL+YGDVFKRIMEH
Sbjct: 302 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLHYGDVFKRIMEH 361

Query: 301 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 360
           SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD
Sbjct: 362 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 421

Query: 361 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDS 420
           SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAK KGLCKNGDS
Sbjct: 422 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKSKGLCKNGDS 481

Query: 421 VVALHRVGTASVIKILTVK 439
           VVALHRVGTASVIKILTVK
Sbjct: 482 VVALHRVGTASVIKILTVK 500

BLAST of Csor.00g223810 vs. ExPASy TrEMBL
Match: A0A6J1H3K9 (Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111459794 PE=3 SV=1)

HSP 1 Score: 850 bits (2197), Expect = 4.52e-310
Identity = 439/439 (100.00%), Postives = 439/439 (100.00%), Query Frame = 0

Query: 1   MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 60
           MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL
Sbjct: 62  MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 121

Query: 61  AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 120
           AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT
Sbjct: 122 AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 181

Query: 121 EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 180
           EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD
Sbjct: 182 EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 241

Query: 181 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 240
           DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT
Sbjct: 242 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 301

Query: 241 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 300
           RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH
Sbjct: 302 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 361

Query: 301 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 360
           SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD
Sbjct: 362 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 421

Query: 361 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDS 420
           SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDS
Sbjct: 422 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDS 481

Query: 421 VVALHRVGTASVIKILTVK 439
           VVALHRVGTASVIKILTVK
Sbjct: 482 VVALHRVGTASVIKILTVK 500

BLAST of Csor.00g223810 vs. ExPASy TrEMBL
Match: A0A6J1K6R4 (Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111491658 PE=3 SV=1)

HSP 1 Score: 848 bits (2191), Expect = 3.71e-309
Identity = 437/439 (99.54%), Postives = 439/439 (100.00%), Query Frame = 0

Query: 1   MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 60
           MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL
Sbjct: 62  MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 121

Query: 61  AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 120
           AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT
Sbjct: 122 AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 181

Query: 121 EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 180
           EKDKEDIL+WGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD
Sbjct: 182 EKDKEDILEWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 241

Query: 181 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 240
           DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT
Sbjct: 242 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 301

Query: 241 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 300
           RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH
Sbjct: 302 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 361

Query: 301 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 360
           SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD
Sbjct: 362 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 421

Query: 361 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDS 420
           SFDWSCSDEAPARHSLIFRGLVPVLSTASA+SSHAETTEEAIEFAIQHAKLKGLCKNGDS
Sbjct: 422 SFDWSCSDEAPARHSLIFRGLVPVLSTASAKSSHAETTEEAIEFAIQHAKLKGLCKNGDS 481

Query: 421 VVALHRVGTASVIKILTVK 439
           VVALHRVGTASVIKILTVK
Sbjct: 482 VVALHRVGTASVIKILTVK 500

BLAST of Csor.00g223810 vs. ExPASy TrEMBL
Match: A0A5D3DE01 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G001150 PE=3 SV=1)

HSP 1 Score: 837 bits (2161), Expect = 1.38e-304
Identity = 432/439 (98.41%), Postives = 435/439 (99.09%), Query Frame = 0

Query: 1   MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 60
           MENTGILCAVMLDTKGPEIRTGFLKDGKPIQL QGQEITISTDYSLKGDENMICMSYKKL
Sbjct: 62  MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYSLKGDENMICMSYKKL 121

Query: 61  AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 120
           AEDVKPGSVILCSDGTISFSVLSCDK+ GLVRCRCENSAVLGERKNVNLPGVIVDLPTLT
Sbjct: 122 AEDVKPGSVILCSDGTISFSVLSCDKKLGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 181

Query: 121 EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 180
           EKDKEDIL+WGVPN IDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD
Sbjct: 182 EKDKEDILEWGVPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 241

Query: 181 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 240
           DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT
Sbjct: 242 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 301

Query: 241 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 300
           RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTL+YGDVFKRIMEH
Sbjct: 302 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLHYGDVFKRIMEH 361

Query: 301 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 360
           SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD
Sbjct: 362 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 421

Query: 361 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDS 420
           SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAK KGLCKNGDS
Sbjct: 422 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKSKGLCKNGDS 481

Query: 421 VVALHRVGTASVIKILTVK 439
           VVALHRVGTASVIKILTVK
Sbjct: 482 VVALHRVGTASVIKILTVK 500

BLAST of Csor.00g223810 vs. ExPASy TrEMBL
Match: A0A1S3BN43 (Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103491640 PE=3 SV=1)

HSP 1 Score: 837 bits (2161), Expect = 1.38e-304
Identity = 432/439 (98.41%), Postives = 435/439 (99.09%), Query Frame = 0

Query: 1   MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 60
           MENTGILCAVMLDTKGPEIRTGFLKDGKPIQL QGQEITISTDYSLKGDENMICMSYKKL
Sbjct: 62  MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYSLKGDENMICMSYKKL 121

Query: 61  AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 120
           AEDVKPGSVILCSDGTISFSVLSCDK+ GLVRCRCENSAVLGERKNVNLPGVIVDLPTLT
Sbjct: 122 AEDVKPGSVILCSDGTISFSVLSCDKKLGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 181

Query: 121 EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 180
           EKDKEDIL+WGVPN IDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD
Sbjct: 182 EKDKEDILEWGVPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 241

Query: 181 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 240
           DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT
Sbjct: 242 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 301

Query: 241 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 300
           RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTL+YGDVFKRIMEH
Sbjct: 302 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLHYGDVFKRIMEH 361

Query: 301 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 360
           SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD
Sbjct: 362 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 421

Query: 361 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDS 420
           SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAK KGLCKNGDS
Sbjct: 422 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKSKGLCKNGDS 481

Query: 421 VVALHRVGTASVIKILTVK 439
           VVALHRVGTASVIKILTVK
Sbjct: 482 VVALHRVGTASVIKILTVK 500

BLAST of Csor.00g223810 vs. ExPASy TrEMBL
Match: A0A0A0L1N0 (Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_4G268000 PE=3 SV=1)

HSP 1 Score: 835 bits (2157), Expect = 5.61e-304
Identity = 431/439 (98.18%), Postives = 435/439 (99.09%), Query Frame = 0

Query: 1   MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 60
           MENTGILCAVMLDTKGPEIRTGFLKDGKPIQL QGQEITISTDYSLKGDENMICMSYKKL
Sbjct: 62  MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYSLKGDENMICMSYKKL 121

Query: 61  AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 120
           AEDVKPGSVILCSDGTISFSVLSCDK+ GLV+CRCENSAVLGERKNVNLPGVIVDLPTLT
Sbjct: 122 AEDVKPGSVILCSDGTISFSVLSCDKKLGLVQCRCENSAVLGERKNVNLPGVIVDLPTLT 181

Query: 121 EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 180
           EKDKEDIL+WGVPN IDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD
Sbjct: 182 EKDKEDILEWGVPNKIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 241

Query: 181 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 240
           DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT
Sbjct: 242 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 301

Query: 241 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 300
           RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTL+YGDVFKRIMEH
Sbjct: 302 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMEH 361

Query: 301 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 360
           SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD
Sbjct: 362 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 421

Query: 361 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDS 420
           SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAK KGLCKNGDS
Sbjct: 422 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKSKGLCKNGDS 481

Query: 421 VVALHRVGTASVIKILTVK 439
           VVALHRVGTASVIKILTVK
Sbjct: 482 VVALHRVGTASVIKILTVK 500

BLAST of Csor.00g223810 vs. TAIR 10
Match: AT5G56350.1 (Pyruvate kinase family protein )

HSP 1 Score: 773.5 bits (1996), Expect = 9.3e-224
Identity = 394/439 (89.75%), Postives = 412/439 (93.85%), Query Frame = 0

Query: 1   MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 60
           M NTGILCAVMLDTKGPEIRTGFLKDGKPIQL QGQEITISTDY LKGDEN ICMSYKKL
Sbjct: 60  MLNTGILCAVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYDLKGDENTICMSYKKL 119

Query: 61  AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 120
           A DV PG VILC+DGTIS  VLSCDKE+G VRCRCENSA+LGERKNVNLPGV+VDLPTLT
Sbjct: 120 AVDVNPGMVILCADGTISLLVLSCDKENGTVRCRCENSAMLGERKNVNLPGVVVDLPTLT 179

Query: 121 EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 180
           EKDKEDI+QWGVPN IDMIALSFVRKGSDLV+VRKLLGKHAK+ILLMSKVENQEGVANFD
Sbjct: 180 EKDKEDIMQWGVPNQIDMIALSFVRKGSDLVQVRKLLGKHAKNILLMSKVENQEGVANFD 239

Query: 181 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 240
           DIL NSDAFM+ARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT
Sbjct: 240 DILVNSDAFMIARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 299

Query: 241 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 300
           RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTL+YGDVFKRIM +
Sbjct: 300 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMLY 359

Query: 301 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 360
           SPVPMSPLESLASSAVRTANSA+A LI+VLTRGGSTA+LVAKYRPG PILSVVVPEIKTD
Sbjct: 360 SPVPMSPLESLASSAVRTANSARATLIMVLTRGGSTARLVAKYRPGMPILSVVVPEIKTD 419

Query: 361 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDS 420
            FDWSCSDE+PARHSLIFRGL+PVL   SAR+SH E+TEEAIEFA Q+ K K LCK GDS
Sbjct: 420 FFDWSCSDESPARHSLIFRGLIPVLYAGSARASHDESTEEAIEFATQYGKEKELCKTGDS 479

Query: 421 VVALHRVGTASVIKILTVK 440
           VVAL RVG ASVIKILTVK
Sbjct: 480 VVALLRVGNASVIKILTVK 498

BLAST of Csor.00g223810 vs. TAIR 10
Match: AT4G26390.1 (Pyruvate kinase family protein )

HSP 1 Score: 741.5 bits (1913), Expect = 3.9e-214
Identity = 373/439 (84.97%), Postives = 403/439 (91.80%), Query Frame = 0

Query: 1   MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 60
           M NTG+LCAVMLDTKGPEIRTGFLKDGKPIQL QGQEITISTDY LKGDE  ICMSYKKL
Sbjct: 59  MLNTGMLCAVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYDLKGDEKTICMSYKKL 118

Query: 61  AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 120
           A+DV PG VILC+DGTIS  VLSCDKE G VRCRCEN+++LGERKNVNLPGV+VDLPTLT
Sbjct: 119 AQDVNPGMVILCADGTISLKVLSCDKEKGTVRCRCENTSMLGERKNVNLPGVVVDLPTLT 178

Query: 121 EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 180
           EKDK+DIL+WGVPN IDMIALSFVRKGSDLV+VRKLLGKHAK+ILLMSKVENQEGVANFD
Sbjct: 179 EKDKQDILEWGVPNQIDMIALSFVRKGSDLVQVRKLLGKHAKTILLMSKVENQEGVANFD 238

Query: 181 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 240
           DIL NSDAFM+ARGDLGMEIPIEKIFLAQKVMIYKCN  GKPVVTATQMLESMIKSPRPT
Sbjct: 239 DILINSDAFMIARGDLGMEIPIEKIFLAQKVMIYKCNFMGKPVVTATQMLESMIKSPRPT 298

Query: 241 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 300
           RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTL+YGD+FKRIM H
Sbjct: 299 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDIFKRIMLH 358

Query: 301 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 360
           + VPMSP+ESLASSAVRTA S++A L++VLTRGGSTA+LVAKYRPG PILSVVVPEI +D
Sbjct: 359 AAVPMSPMESLASSAVRTATSSRATLMMVLTRGGSTARLVAKYRPGIPILSVVVPEITSD 418

Query: 361 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDS 420
           SFDW+CS+EAPARHSLI+RGLVPVL   SAR+S  E+TEE +EFA ++ K K LCK GDS
Sbjct: 419 SFDWACSNEAPARHSLIYRGLVPVLYAGSARASIDESTEETLEFASEYGKKKQLCKTGDS 478

Query: 421 VVALHRVGTASVIKILTVK 440
           VVAL R G A VIKILTVK
Sbjct: 479 VVALFRTGNAIVIKILTVK 497

BLAST of Csor.00g223810 vs. TAIR 10
Match: AT5G08570.1 (Pyruvate kinase family protein )

HSP 1 Score: 689.5 bits (1778), Expect = 1.8e-198
Identity = 342/439 (77.90%), Postives = 390/439 (88.84%), Query Frame = 0

Query: 1   MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 60
           M NTGIL AVMLDTKGPEIRTGFLKDG PIQL +GQEITI+TDY ++GDE+ I MSYKKL
Sbjct: 72  MHNTGILAAVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITTDYDIQGDESTISMSYKKL 131

Query: 61  AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 120
             DVKPG+ ILC+DG+IS +VLSCD ESG VRCRCENSA+LGERKNVNLPGV+VDLPTLT
Sbjct: 132 PLDVKPGNTILCADGSISLAVLSCDPESGTVRCRCENSAMLGERKNVNLPGVVVDLPTLT 191

Query: 121 EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 180
           +KD EDIL WGVPN+IDMIALSFVRKGSDLV VRK+LG HAKSI+LMSKVENQEGV NFD
Sbjct: 192 DKDIEDILGWGVPNSIDMIALSFVRKGSDLVNVRKVLGSHAKSIMLMSKVENQEGVINFD 251

Query: 181 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 240
           +IL  +DAFMVARGDLGMEIPIEKIFLAQK+MIYKCN+ GKPVVTATQMLESMIKSPRPT
Sbjct: 252 EILRETDAFMVARGDLGMEIPIEKIFLAQKLMIYKCNLAGKPVVTATQMLESMIKSPRPT 311

Query: 241 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 300
           RAEATDVANAVLDGTDCVMLSGE+AAGAYPE+AV+ MAKIC+EAES+L+Y  +FK ++  
Sbjct: 312 RAEATDVANAVLDGTDCVMLSGESAAGAYPEIAVKVMAKICIEAESSLDYNTIFKEMIRA 371

Query: 301 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 360
           +P+PMSPLESLASSAVRTAN A+A LI+VLTRGGSTA LVAKYRP  PILSVVVP + TD
Sbjct: 372 TPLPMSPLESLASSAVRTANKARAKLIIVLTRGGSTANLVAKYRPAVPILSVVVPVMTTD 431

Query: 361 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDS 420
           SFDWSCSDE+PARHSLI+RGL+P+L+  SA+++ +E TE  IE A++ A  +GLC  GD+
Sbjct: 432 SFDWSCSDESPARHSLIYRGLIPMLAEGSAKATDSEATEVIIEAALKSATQRGLCNRGDA 491

Query: 421 VVALHRVGTASVIKILTVK 440
           +VALHR+G ASVIKI  VK
Sbjct: 492 IVALHRIGAASVIKICVVK 510

BLAST of Csor.00g223810 vs. TAIR 10
Match: AT5G63680.1 (Pyruvate kinase family protein )

HSP 1 Score: 686.8 bits (1771), Expect = 1.1e-197
Identity = 340/439 (77.45%), Postives = 391/439 (89.07%), Query Frame = 0

Query: 1   MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 60
           M+NTGIL AVMLDTKGPEIRTGFLKDG PIQL +GQEITI+TDY +KGDE  I MSYKKL
Sbjct: 72  MQNTGILAAVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITTDYDIKGDEKTISMSYKKL 131

Query: 61  AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 120
             DVKPG+ ILC+DG+IS +V+SCD  +G V CRCEN+A+LGERKNVNLPGV+VDLPTLT
Sbjct: 132 PVDVKPGNTILCADGSISLAVVSCDPNAGTVICRCENTAMLGERKNVNLPGVVVDLPTLT 191

Query: 121 EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 180
           +KD EDIL+WGVPNNIDMIALSFVRKGSDLV VRK+LG H+KSI+LMSKVENQEGV NFD
Sbjct: 192 DKDVEDILKWGVPNNIDMIALSFVRKGSDLVNVRKVLGSHSKSIMLMSKVENQEGVLNFD 251

Query: 181 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 240
           +IL  +DAFMVARGDLGMEIPIEKIFLAQK+MIYKCN+ GKPVVTATQMLESMIKSPRPT
Sbjct: 252 EILRETDAFMVARGDLGMEIPIEKIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPT 311

Query: 241 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 300
           RAEATDVANAVLDGTDCVMLSGE+AAGAYPE+AV+TMAKIC+EAES+L+Y  +FK ++  
Sbjct: 312 RAEATDVANAVLDGTDCVMLSGESAAGAYPEIAVKTMAKICIEAESSLDYNTIFKEMIRA 371

Query: 301 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTD 360
           +P+PMS LESLASSAVRTAN AKA LI+VLTRGG+TAKLVAKYRP  PILSVVVP   +D
Sbjct: 372 TPLPMSTLESLASSAVRTANKAKAKLIIVLTRGGTTAKLVAKYRPAVPILSVVVPVFTSD 431

Query: 361 SFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKLKGLCKNGDS 420
           +F+WSCSDE+PARHSLI+RGL+PVL   SA+++ +E+TEE IE A++ A  KGLC +GD+
Sbjct: 432 TFNWSCSDESPARHSLIYRGLIPVLGEGSAKATDSESTEEIIESALKSATEKGLCNHGDA 491

Query: 421 VVALHRVGTASVIKILTVK 440
           VVALHR+G ASVIKI  VK
Sbjct: 492 VVALHRIGAASVIKICVVK 510

BLAST of Csor.00g223810 vs. TAIR 10
Match: AT3G04050.1 (Pyruvate kinase family protein )

HSP 1 Score: 644.0 bits (1660), Expect = 8.5e-185
Identity = 330/443 (74.49%), Postives = 383/443 (86.46%), Query Frame = 0

Query: 1   MENTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKL 60
           MENT I CAVMLDTKGPEIRTGFLK+GKP++L QGQEITISTDY+++GD N I MSYKKL
Sbjct: 68  MENTCIPCAVMLDTKGPEIRTGFLKEGKPVELIQGQEITISTDYTMEGDSNTISMSYKKL 127

Query: 61  AEDVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLT 120
           AED+K G VILCSDGTIS +VLSCDK  GLVR RCENSAVLGERKNVNLPG++VDLPTLT
Sbjct: 128 AEDLKSGDVILCSDGTISLTVLSCDKNLGLVRARCENSAVLGERKNVNLPGIVVDLPTLT 187

Query: 121 EKDKEDILQWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFD 180
           EKD+EDILQWGVPN ID+IALSFVRKGSDLVEVRKLLG++AKSI+LMSKVENQEGV NFD
Sbjct: 188 EKDQEDILQWGVPNKIDIIALSFVRKGSDLVEVRKLLGENAKSIMLMSKVENQEGVMNFD 247

Query: 181 DILANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPT 240
            IL  SDAFMVARGDLGMEIPIEK+FLAQK+MI K N  GKP+VTATQMLESM KSPRPT
Sbjct: 248 KILEYSDAFMVARGDLGMEIPIEKMFLAQKMMIQKANALGKPIVTATQMLESMTKSPRPT 307

Query: 241 RAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEH 300
           RAEATDVANAVLDGTDCVMLSGETAAGA+PE AV TM++IC EAE  ++Y  + K+I + 
Sbjct: 308 RAEATDVANAVLDGTDCVMLSGETAAGAHPETAVLTMSRICKEAEDFIDYDTMHKKIQDI 367

Query: 301 SPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEI-KT 360
             +P+SP+ESLA+SAV TA S  AA I+VLT+GG T +LVAKYRP  PILSV+VPEI +T
Sbjct: 368 VSLPLSPIESLAASAVSTARSLCAAAIVVLTKGGYTVELVAKYRPSVPILSVIVPEITRT 427

Query: 361 DSFDWSCSDEAP--ARHSLIFRGLVPVLST-ASARSSHAETTEEAIEFAIQHAKLKGLCK 420
           D F+WSCS+ A   AR  LI+RG+VPV++T ASARSS+ ++TEE I+FAI+ AK KG+CK
Sbjct: 428 DDFEWSCSESAAHVARRGLIYRGIVPVMATGASARSSNKDSTEETIQFAIEFAKKKGICK 487

Query: 421 NGDSVVALHRVGTASVIKILTVK 440
            GDS+VALH++  +SV+KIL V+
Sbjct: 488 TGDSIVALHKIDGSSVVKILNVE 510

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q429541.6e-22589.75Pyruvate kinase, cytosolic isozyme OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
O655955.5e-21384.97Probable pyruvate kinase, cytosolic isozyme OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q428062.2e-19878.13Pyruvate kinase, cytosolic isozyme OS=Glycine max OX=3847 PE=2 SV=1[more]
P222009.7e-19476.54Pyruvate kinase, cytosolic isozyme OS=Solanum tuberosum OX=4113 PE=2 SV=1[more]
O440062.1e-10848.08Pyruvate kinase OS=Eimeria tenella OX=5802 GN=PYK PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6605827.19.29e-311100.00hypothetical protein SDJN03_03144, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022958623.19.33e-310100.00pyruvate kinase, cytosolic isozyme [Cucurbita moschata] >KAG7035785.1 hypothetic... [more]
XP_022996430.17.65e-30999.54pyruvate kinase, cytosolic isozyme [Cucurbita maxima][more]
XP_023533624.11.09e-30899.77pyruvate kinase, cytosolic isozyme [Cucurbita pepo subsp. pepo][more]
XP_008449903.12.85e-30498.41PREDICTED: pyruvate kinase, cytosolic isozyme [Cucumis melo] >KAA0040121.1 pyruv... [more]
Match NameE-valueIdentityDescription
A0A6J1H3K94.52e-310100.00Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111459794 PE=3 SV=1[more]
A0A6J1K6R43.71e-30999.54Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111491658 PE=3 SV=1[more]
A0A5D3DE011.38e-30498.41Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G0011... [more]
A0A1S3BN431.38e-30498.41Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103491640 PE=3 SV=1[more]
A0A0A0L1N05.61e-30498.18Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_4G268000 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G56350.19.3e-22489.75Pyruvate kinase family protein [more]
AT4G26390.13.9e-21484.97Pyruvate kinase family protein [more]
AT5G08570.11.8e-19877.90Pyruvate kinase family protein [more]
AT5G63680.11.1e-19777.45Pyruvate kinase family protein [more]
AT3G04050.18.5e-18574.49Pyruvate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001697Pyruvate kinasePRINTSPR01050PYRUVTKNASEcoord: 6..22
score: 62.9
coord: 243..261
score: 77.25
coord: 262..278
score: 58.37
coord: 136..150
score: 51.79
coord: 166..192
score: 51.68
coord: 218..242
score: 72.25
coord: 193..217
score: 63.51
IPR001697Pyruvate kinaseTIGRFAMTIGR01064TIGR01064coord: 4..428
e-value: 1.9E-130
score: 433.5
IPR001697Pyruvate kinasePANTHERPTHR11817PYRUVATE KINASEcoord: 1..439
IPR015795Pyruvate kinase, C-terminalPFAMPF02887PK_Ccoord: 309..435
e-value: 9.9E-22
score: 77.4
IPR015793Pyruvate kinase, barrelPFAMPF00224PKcoord: 2..292
e-value: 3.4E-129
score: 430.7
IPR040442Pyruvate kinase-like domain superfamilyGENE3D3.20.20.60coord: 3..284
e-value: 4.8E-167
score: 558.2
IPR015806Pyruvate kinase, insert domain superfamilyGENE3D2.40.33.10coord: 17..117
e-value: 4.8E-167
score: 558.2
IPR036918Pyruvate kinase, C-terminal domain superfamilyGENE3D3.40.1380.20coord: 285..426
e-value: 4.8E-167
score: 558.2
IPR036918Pyruvate kinase, C-terminal domain superfamilySUPERFAMILY52935PK C-terminal domain-likecoord: 273..438
NoneNo IPR availablePANTHERPTHR11817:SF90PYRUVATE KINASEcoord: 1..439
IPR018209Pyruvate kinase, active sitePROSITEPS00110PYRUVATE_KINASEcoord: 164..176
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILY51621Phosphoenolpyruvate/pyruvate domaincoord: 6..290
IPR011037Pyruvate kinase-like, insert domain superfamilySUPERFAMILY50800PK beta-barrel domain-likecoord: 17..116

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g223810.m01Csor.00g223810.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0016301 kinase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030955 potassium ion binding
molecular_function GO:0004743 pyruvate kinase activity
molecular_function GO:0003824 catalytic activity