Csor.00g222420 (gene) Silver-seed gourd (wild; sororia) v1

BLAST of Csor.00g222420 vs. ExPASy Swiss-Prot
Match: Q0WPH8 (Serine/threonine-protein kinase Nek5 OS=Arabidopsis thaliana OX=3702 GN=NEK5 PE=1 SV=1)

HSP 1 Score: 780.8 bits (2015), Expect = 1.5e-224
Identity = 486/946 (51.37%), Postives = 582/946 (61.52%), Query Frame = 0

Query: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMD YE+MEQIGRGAFGAAILVHHK+E+KKYVLKKIRLARQTERCRRSAHQEM+LIA
Sbjct: 1   MESRMDQYELMEQIGRGAFGAAILVHHKAERKKYVLKKIRLARQTERCRRSAHQEMSLIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMA+LMKKSNG++FPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGVYFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHS++VLHRDLKCSNIFLTKD+DVRLGDFGLAKTLKADDL SSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNYVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLTSSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCC+YEMAA RPAFKAFDMAGLISK+NRSSIGPLP CYSPSLK LIK
Sbjct: 181 DIPYGFKSDIWSLGCCIYEMAAYRPAFKAFDMAGLISKVNRSSIGPLPPCYSPSLKALIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS 300
           GMLRKNPE+RPNASE+LKHPYLQPYVEQYRP+++     +P+K   +R+ RR MAESQNS
Sbjct: 241 GMLRKNPEYRPNASEILKHPYLQPYVEQYRPTLS-AASITPEKPLNSREGRRSMAESQNS 300

Query: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEE-QTHNTCSL 360
           NSS S++D+   SDKN   +  +  ++VT+ D   VDD D  + +  S E     +  + 
Sbjct: 301 NSS-SEKDNFYVSDKNIRYVVPSNGNKVTETDSGFVDDEDILDHVQQSAENGNLQSVSAT 360

Query: 361 KVNDPMHSKPAQADHQSNT-ESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNH 420
           K +     KP  +D + +  + R PKTIRNIM+ LKE KAREN SP+R N  +       
Sbjct: 361 KPDGHGILKPVHSDQRPDVIQPRHPKTIRNIMMVLKEEKARENGSPMRSNRSRPSSVPTQ 420

Query: 421 KAHAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSK 480
           K + E P K+ K       SK+NA TP  +P       S R      PKH +PV+D + K
Sbjct: 421 KNNVETPSKIPKLGDIAHSSKTNASTP--IPPSKLASDSARTPGSFPPKHHMPVIDSSPK 480

Query: 481 TKPRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPA-RARQAELNVPD-AVSST 540
            KPR D   P        ++ +S K RQRTPPT+ RR +  A ++RQ   ++ + A   T
Sbjct: 481 LKPRNDRISPSPAAKHEAEEAMSVKRRQRTPPTLPRRTSLIAHQSRQLGADISNMAAKET 540

Query: 541 EMIH-----QKETQ-----------HTTSEP------FSKGMQTDSSNSVSSSVSIQGFE 600
             +H     + ET             TT EP       +KGMQ++SSNS+SSS+S+Q FE
Sbjct: 541 AKLHPSVPSESETNSHQSRVHASPVSTTPEPKRTSVGSAKGMQSESSNSISSSLSMQAFE 600

Query: 601 ICDDAATPFTDIPELVL--DHQSIEHDELEETHPP------------------------- 660
           +CDDA+TP+ D+ E     DH+   H E   + P                          
Sbjct: 601 LCDDASTPYIDMTEHTTPDDHRRSCHSEYSYSFPDISSEMMIRRDEHSTSMRLTEIPDSV 660

Query: 661 -----------------SCSSSL----------------QYEKGN------------PGV 720
                            SC + L                Q++ G+            PG 
Sbjct: 661 SGVQNTIAHHQPEREQGSCPTVLKDDSPATLQSYEPNTSQHQHGDDKFTVKEFVSSVPGP 720

Query: 721 DDKPL-----------SDKVTSL---NNPSSEKLASSHLPAAFDDVIHVIRHSSFRVGSE 780
              PL           SD  TS+   N+   +  + SH     DDVIHVIRHSSFRVGS+
Sbjct: 721 APLPLHVEPSHQVNSHSDNKTSVMSQNSALEKNNSHSHPHPVVDDVIHVIRHSSFRVGSD 780

BLAST of Csor.00g222420 vs. ExPASy Swiss-Prot
Match: Q94CU5 (Serine/threonine-protein kinase Nek5 OS=Oryza sativa subsp. japonica OX=39947 GN=NEK5 PE=2 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 5.6e-184
Identity = 431/951 (45.32%), Postives = 543/951 (57.10%), Query Frame = 0

Query: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           M+SRMD YE+MEQIGRGAFGAAILV+HK+EKKKYVLKKIRLARQTERCR+SAHQEMALIA
Sbjct: 1   MDSRMDQYEVMEQIGRGAFGAAILVNHKTEKKKYVLKKIRLARQTERCRKSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120
           R+QHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMA+LMKK+NG +FPEEKL KWF QL LA
Sbjct: 61  RLQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKANGTYFPEEKLLKWFAQLALA 120

Query: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           V+YLHS+FVLHRDLKCSNIFLTKD+D+RLGDFGLAKTLKADDL SSVVGTPNYMCPELLA
Sbjct: 121 VDYLHSNFVLHRDLKCSNIFLTKDQDIRLGDFGLAKTLKADDLTSSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAA RPAFKAFDMAGLISKINRSSIGPLP CYSPS+K+LIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPPCYSPSMKSLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRP--SIAPTVDFSPDKRRPTRDSRRCMAESQ 300
            MLRK+PEHRP ASE+LK PYLQPYV QYRP   I+  +         +R S++ M+ SQ
Sbjct: 241 SMLRKSPEHRPTASEILKSPYLQPYVNQYRPFADISHPIHSLEKPITSSRSSQKSMSGSQ 300

Query: 301 NSNSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTH-NTC 360
            S+ S SD DS+ SS++N    +++ ++ + D + A   DH   +    S++ +++  T 
Sbjct: 301 CSSISGSDIDSIQSSERNTSGPSTSSNNTI-DTEGAEATDHVSVKNCSRSDDVKSNKETV 360

Query: 361 SLKVNDPMHSKPAQADHQSNTESRRPKTIRNIMLALK-EGKARENYSPVRGNCVKSGVAS 420
             ++     SK    D +   E ++PK I+ I+  L+ E K R+N SP+R + VK    S
Sbjct: 361 GPELERQDSSKSIHVDQRPRNEIKQPKIIKKILTTLREESKLRQNNSPIRASRVKLNSPS 420

Query: 421 NHKAHAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPT 480
           N +  ++      K    +  S  +++  +   AK   E  +R QA    KH  P+V+ +
Sbjct: 421 NREQLSDDS----KHSSDISSSSKSSEVTSRESAKVICEPVKRAQASPPLKHLSPIVEHS 480

Query: 481 SKTKPRYDNYPPLFHPVKPV---DDGLSSKSRQRTPPTVLRRPTFPAR---ARQAELNVP 540
            K K + D  P    P K      D    K + RTPP+  RR + P R     ++ L+  
Sbjct: 481 PKAKIKQDE-PLQPDPAKQAMEDVDAAVGKVKNRTPPSYSRRLSIPPRRPLGAESPLHAD 540

Query: 541 DAVSSTEMIHQK-------------------------------------ETQHTTSEPFS 600
              +  ++I ++                                       +  ++ P +
Sbjct: 541 TKRAHNKVIKERAKSPCRPVHGPDNDIIEPPGFPMAPPSPLGGVQMKVGNARAKSAPPRA 600

Query: 601 KGMQTDSSNSVSSSVS-------------------------------------------- 660
             ++ DSS+  SS+++                                            
Sbjct: 601 VSIKEDSSDCSSSTIAYAENTELSEPSKQDSSAQLVSSCKCSIPDAAIQKHDLTAMPSSE 660

Query: 661 ----------IQGFEICDD-AATPFTDIPELV---------------LDHQSIEHDELEE 720
                         ++C++ A  P +DI E V                 +  +++DE + 
Sbjct: 661 LNTTNFQKSMASNDDVCENLALEPSSDISEQVSIFKDNVPCSKISQSTANAIVQNDEDKF 720

Query: 721 THPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIRHSSF 780
           T     SS        P V  K  + +  S    S E+ +S HL    +DVIHVIRHSSF
Sbjct: 721 TVQELLSSVADIA---PFVSTKNFALEKGSPPIQSLERTSSPHLNPPIEDVIHVIRHSSF 780

Query: 781 RVGSEQAATENLE--DRSS---------CKEMDGGSPP---------------------- 785
           RV  EQA  EN E   +SS          +E+D  S P                      
Sbjct: 781 RVCGEQAVAENAEMGVQSSDVGKLLNVVREEVDSRSIPSNNLVPHRLPDCAAPKPNISET 840

BLAST of Csor.00g222420 vs. ExPASy Swiss-Prot
Match: Q60DG4 (Serine/threonine-protein kinase Nek4 OS=Oryza sativa subsp. japonica OX=39947 GN=NEK4 PE=2 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 2.1e-170
Identity = 424/947 (44.77%), Postives = 521/947 (55.02%), Query Frame = 0

Query: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMD YEIMEQ+GRGAFGAAILV+HK E+KKYVLKKIRLARQTERCR+SAHQEMALIA
Sbjct: 1   MESRMDQYEIMEQVGRGAFGAAILVNHKIERKKYVLKKIRLARQTERCRKSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120
           R+QHPYIVEFKEAWVEKGCYVCIVTGYCEGGDM +LMKK NG +FPEEKL KWF QL+LA
Sbjct: 61  RLQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMDELMKKLNGTYFPEEKLLKWFAQLVLA 120

Query: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           V+YLHS++VLHRDLKCSNIFLTKD+D+RLGDFGLAKTLK DDL SSVVGTPNYMCPELL 
Sbjct: 121 VDYLHSNYVLHRDLKCSNIFLTKDQDIRLGDFGLAKTLKEDDLTSSVVGTPNYMCPELLT 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAA RPAFKAFDMAGLISKINRSSIGPLP+CYS S+KTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPACYSSSMKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRP-SIAPTVDFSPDKRRPT-RDSRRCMAESQ 300
            MLRK+PEHRP ASE+LK+PYLQPYV Q RP S APT    P+K   T R ++RC +ESQ
Sbjct: 241 SMLRKSPEHRPTASEILKNPYLQPYVNQCRPLSDAPTPIRMPEKPLSTSRSNQRCTSESQ 300

Query: 301 NSNSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHD----GPELLVPSEEEQTH 360
           +S+ S SD DS  SSD++    A + D ++  ND+ S+ D D      + + P +     
Sbjct: 301 SSSISCSDIDSTQSSDRSTSGGAPSTDSKL--NDIRSIQDADRADSDEKCVTPEDLRGNK 360

Query: 361 NTCSLKVNDPMHSKPAQADHQSNTESRRPKTIRNIMLALK-EGKARENYSPVRGNCVKSG 420
           N    ++     SK     H+   ES++PK I  IM  L+ E + REN SPV  + VK  
Sbjct: 361 NISGAELKRQDSSKSVHQHHRG--ESKQPKIIEKIMTTLREESRLRENNSPVSSSGVKLT 420

Query: 421 VASNHKAHAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVV 480
            A ++K  AE   +  +P   V  S    D  +      + E    +Q     K   P V
Sbjct: 421 SAVSNKNQAEQSSESSRPHSGVSYSSKFGDISSNGWTNTSDECVDPVQVPLQLKQLSPTV 480

Query: 481 DPTSKTKPRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELN-VPDA 540
           +   K K    + P     +       S  S+ ++ P+  RRP+ P R   A +  VP  
Sbjct: 481 EHCPKLKNSGSSTPEPAKQIAENGSSASGMSKTKSSPSSSRRPS-PQRQTVAGIPIVPFT 540

Query: 541 VSSTEMIHQKETQ------HTTSE---------PFSKGMQTDS----------------- 600
           VS    I  +  +      H+ +          P S  +  ++                 
Sbjct: 541 VSKRAHIKAESEKTPPRPAHSPNNSLHNLPPLIPISTNLSEENIKLGNSQAMPAPLEFVT 600

Query: 601 ----------SNSVSSSVS----IQGFEICDDA--------------------ATPFTDI 660
                     SNSV   V      + FE    A                    A P ++I
Sbjct: 601 AASKEDISFYSNSVVDCVEKAEPSEVFESNSPAYLTPPWTGPVLDAKGENGLIAIPCSEI 660

Query: 661 PELVLDHQSIEHDELEETHPPS-----------CSSSLQYEK-GNPGV-----DDK---- 720
               L      +D+   + P             C    Q  K G+  V     +DK    
Sbjct: 661 HTGTLQKSMASNDDSSLSSPLDTFYLSFEQEFVCKDDSQSSKHGHSAVTLLSGEDKFTVQ 720

Query: 721 -------PLSDKVTSLNN---------PSSEKLASSHLPAAFDDVIHVIRHSSFRVGSEQ 780
                   +S  V+S +N          S +K + SH     D     IR +SF V  E 
Sbjct: 721 ELLASTPVISPFVSSTSNTLPEDKSSYQSFKKQSDSHSGPPVDVPAQTIRLNSFLVSDEW 780

Query: 781 AATENLEDR---------------------SSCK---EMDGGSP---------------- 785
             +E ++                       SSC    +  G +P                
Sbjct: 781 PTSETVQGEARDTAASKLLNVVREDFDVRSSSCSTSTQPSGQTPVRSKLNVPETNLASNI 840

BLAST of Csor.00g222420 vs. ExPASy Swiss-Prot
Match: Q2QMH1 (Serine/threonine-protein kinase Nek2 OS=Oryza sativa subsp. japonica OX=39947 GN=NEK2 PE=2 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 4.4e-120
Identity = 303/772 (39.25%), Postives = 402/772 (52.07%), Query Frame = 0

Query: 5   MDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIARVQH 64
           MD YE++EQIG+GAFG+A+LV HK EKKKYVLKKIRLARQT+R RRSAHQEM LIA V++
Sbjct: 1   MDQYEVLEQIGKGAFGSALLVRHKLEKKKYVLKKIRLARQTDRTRRSAHQEMQLIATVRN 60

Query: 65  PYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLAVEYL 124
           P+IVE+K++WVEKGCYVCIV GYCEGGDMA+ +K++NG +F EEKLCKW  QLL+A++YL
Sbjct: 61  PFIVEYKDSWVEKGCYVCIVIGYCEGGDMAEAIKRANGTYFSEEKLCKWLVQLLMALDYL 120

Query: 125 HSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLADIPY 184
           H++ +LHRD+KCSNIF+ +D+ +RLGDFGLAK L +DDLASSVVGTP+YMCPELLADIPY
Sbjct: 121 HANHILHRDVKCSNIFIARDQSIRLGDFGLAKILTSDDLASSVVGTPSYMCPELLADIPY 180

Query: 185 GFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIKGMLR 244
           G KSDIWSLGCC+YEM A RPAFKAFDM  LI+KI +S + PLP+ YS + + LIK MLR
Sbjct: 181 GTKSDIWSLGCCIYEMTALRPAFKAFDMQALINKITKSIVSPLPTKYSGAFRGLIKSMLR 240

Query: 245 KNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNSNSSN 304
           K+PEHRP+A++LLKHP LQPYV Q +   +PT                            
Sbjct: 241 KSPEHRPSAAQLLKHPQLQPYVLQVQLKSSPT---------------------------- 300

Query: 305 SDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLKVNDP 364
                     +N   +  +  D+V              ++  PS+           V D 
Sbjct: 301 ----------RNILPIHQSLTDKV-------------KKMTFPSD-----------VVDS 360

Query: 365 MHSKPAQADHQSNTES---RRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKAH 424
              + A+ +   N  +    +P   RN + + +  K       V+G  V S  A +    
Sbjct: 361 ARRRMARRNSLGNERTVTFSKPSPERNSVSSTRSIKEYTTTQSVKGLSVDSSEAGD---- 420

Query: 425 AEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTKP 484
            E+  K I     +  + S   TP ++PAK             T ++QL         +P
Sbjct: 421 -EVTSKAI-----ITKTSSILRTPKSLPAK-----------TYTARNQL---------EP 480

Query: 485 RYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIHQ 544
              +Y   +    P           +T P  + RP     AR+A L +            
Sbjct: 481 PKTSYNRTYRSELP----------SKTTPNKIARP-----ARRASLPL------------ 540

Query: 545 KETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDEL 604
                +T E  +K   +      S  VS+    I   A  P     + +L      H++L
Sbjct: 541 -----STYETPTKRSISILEQLDSPDVSVNAPRIDRIAEFPLASSEDPLLP----IHNKL 587

Query: 605 EETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIRHS 664
              H  SCS+        P ++     DK T                      I V+R  
Sbjct: 601 SPGH-GSCSTP-------PFINRSITKDKCT----------------------IQVLR-- 587

Query: 665 SFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKSF 724
                          D  +  +  G +   A ++ S+ S            ++  D  SF
Sbjct: 661 --------------TDGDNGSDSSGRNATAASSRGSNDSR-----------QQRFDTSSF 587

Query: 725 RQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLK 774
           +QRAEALE LLE SA+LLQ  R EELG++LKPFG +K SPRETAIWL KS K
Sbjct: 721 QQRAEALEGLLEFSAQLLQQERYEELGILLKPFGPEKASPRETAIWLTKSFK 587

BLAST of Csor.00g222420 vs. ExPASy Swiss-Prot
Match: A2ZMH2 (Serine/threonine-protein kinase Nek2 OS=Oryza sativa subsp. indica OX=39946 GN=NEK2 PE=3 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 7.5e-120
Identity = 303/772 (39.25%), Postives = 401/772 (51.94%), Query Frame = 0

Query: 5   MDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIARVQH 64
           MD YE++EQIG+GAFG+A+LV HK EKKKYVLKKIRLARQT+R RRSAHQEM LIA V++
Sbjct: 1   MDQYEVLEQIGKGAFGSALLVRHKLEKKKYVLKKIRLARQTDRTRRSAHQEMQLIATVRN 60

Query: 65  PYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLAVEYL 124
           P+IVE+K++WVEKGCYVCIV GYCEGGDMA+ +K++NG +F EEKLCKW  QLL+A++YL
Sbjct: 61  PFIVEYKDSWVEKGCYVCIVIGYCEGGDMAEAIKRANGTYFSEEKLCKWLVQLLMALDYL 120

Query: 125 HSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLADIPY 184
           H++ +LHRD+KCSNIF+ +D+ +RLGDFGLAK L +DDLASSVVGTP+YMCPELLADIPY
Sbjct: 121 HANHILHRDVKCSNIFIARDQSIRLGDFGLAKILTSDDLASSVVGTPSYMCPELLADIPY 180

Query: 185 GFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIKGMLR 244
           G KSDIWSLGCC+YEM A RPAFKAFDM  LI+KI +S + PLP+ YS + + LIK MLR
Sbjct: 181 GTKSDIWSLGCCIYEMTALRPAFKAFDMQALINKITKSIVSPLPTKYSGAFRGLIKSMLR 240

Query: 245 KNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNSNSSN 304
           K+PEHRP+A++LLKHP LQPYV Q +   +PT                            
Sbjct: 241 KSPEHRPSAAQLLKHPQLQPYVLQVQLKSSPT---------------------------- 300

Query: 305 SDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLKVNDP 364
                     +N   +  +  D+V              ++  PS+           V D 
Sbjct: 301 ----------RNILPIHQSLTDKV-------------KKMTFPSD-----------VVDS 360

Query: 365 MHSKPAQADHQSNTES---RRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKAH 424
              + A+ +   N  +    +P   RN + + +  K       V G  V S  A +    
Sbjct: 361 ARRRMARRNSLGNERTVTFSKPSPERNSVSSTRSIKEYTTTQSVEGLSVDSSEAGD---- 420

Query: 425 AEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTKP 484
            E+  K I     +  + S   TP ++PAK             T ++QL         +P
Sbjct: 421 -EVTSKAI-----ITKTSSILRTPKSLPAK-----------TYTARNQL---------EP 480

Query: 485 RYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIHQ 544
              +Y   +    P           +T P  + RP     AR+A L +            
Sbjct: 481 PKTSYNRTYRSELP----------SKTTPNKIARP-----ARRASLPL------------ 540

Query: 545 KETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDEL 604
                +T E  +K   +      S  VS+    I   A  P     + +L      H++L
Sbjct: 541 -----STYETPTKRSISILEQLDSPDVSVNAPRIDRIAEFPLASSEDPLLP----IHNKL 587

Query: 605 EETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIRHS 664
              H  SCS+        P ++     DK T                      I V+R  
Sbjct: 601 SPGH-GSCSTP-------PFINRSITKDKCT----------------------IQVLR-- 587

Query: 665 SFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKSF 724
                          D  +  +  G +   A ++ S+ S            ++  D  SF
Sbjct: 661 --------------TDGDNGSDSSGRNATAASSRGSNDSR-----------QQRFDTSSF 587

Query: 725 RQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLK 774
           +QRAEALE LLE SA+LLQ  R EELG++LKPFG +K SPRETAIWL KS K
Sbjct: 721 QQRAEALEGLLEFSAQLLQQERYEELGILLKPFGPEKASPRETAIWLTKSFK 587

BLAST of Csor.00g222420 vs. NCBI nr
Match: KAG6605965.1 (Serine/threonine-protein kinase Nek5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1545 bits (4001), Expect = 0.0
Identity = 784/784 (100.00%), Postives = 784/784 (100.00%), Query Frame = 0

Query: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS 300

Query: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360
           NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK
Sbjct: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360

Query: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA 420
           VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA
Sbjct: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA 420

Query: 421 HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480
           HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK
Sbjct: 421 HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480

Query: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH 540
           PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH
Sbjct: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH 540

Query: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE 600
           QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE
Sbjct: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE 600

Query: 601 LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIRH 660
           LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIRH
Sbjct: 601 LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIRH 660

Query: 661 SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS 720
           SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS
Sbjct: 661 SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS 720

Query: 721 FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN 780
           FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN
Sbjct: 721 FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN 780

Query: 781 ARSS 784
           ARSS
Sbjct: 781 ARSS 784

BLAST of Csor.00g222420 vs. NCBI nr
Match: XP_022957727.1 (serine/threonine-protein kinase Nek5-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1540 bits (3988), Expect = 0.0
Identity = 782/784 (99.74%), Postives = 783/784 (99.87%), Query Frame = 0

Query: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHS+FVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRR MAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRSMAESQNS 300

Query: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360
           NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK
Sbjct: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360

Query: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA 420
           VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA
Sbjct: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA 420

Query: 421 HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480
           HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK
Sbjct: 421 HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480

Query: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH 540
           PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH
Sbjct: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH 540

Query: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE 600
           QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE
Sbjct: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE 600

Query: 601 LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIRH 660
           LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIRH
Sbjct: 601 LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIRH 660

Query: 661 SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS 720
           SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS
Sbjct: 661 SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS 720

Query: 721 FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN 780
           FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN
Sbjct: 721 FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN 780

Query: 781 ARSS 784
           ARSS
Sbjct: 781 ARSS 784

BLAST of Csor.00g222420 vs. NCBI nr
Match: XP_023532893.1 (serine/threonine-protein kinase Nek5-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1531 bits (3963), Expect = 0.0
Identity = 777/784 (99.11%), Postives = 780/784 (99.49%), Query Frame = 0

Query: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHS+FVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRR MAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRSMAESQNS 300

Query: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360
           NSSNSDRDSLISSDKNAPAMASNCDD+VTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK
Sbjct: 301 NSSNSDRDSLISSDKNAPAMASNCDDKVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360

Query: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA 420
           VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASN KA
Sbjct: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNQKA 420

Query: 421 HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480
           HAE+PPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK
Sbjct: 421 HAEVPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480

Query: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH 540
           PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPAR RQAELNVPDAVSSTE IH
Sbjct: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARTRQAELNVPDAVSSTERIH 540

Query: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE 600
           QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE
Sbjct: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE 600

Query: 601 LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIRH 660
           LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIRH
Sbjct: 601 LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIRH 660

Query: 661 SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS 720
           SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS
Sbjct: 661 SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS 720

Query: 721 FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN 780
           FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN
Sbjct: 721 FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN 780

Query: 781 ARSS 784
           ARSS
Sbjct: 781 ARSS 784

BLAST of Csor.00g222420 vs. NCBI nr
Match: KAG7035916.1 (Serine/threonine-protein kinase Nek5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1520 bits (3936), Expect = 0.0
Identity = 775/784 (98.85%), Postives = 776/784 (98.98%), Query Frame = 0

Query: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS 300

Query: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360
           NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK
Sbjct: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360

Query: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA 420
           VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA
Sbjct: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA 420

Query: 421 HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480
           HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK
Sbjct: 421 HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480

Query: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH 540
           PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH
Sbjct: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH 540

Query: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE 600
           QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLD       +
Sbjct: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLD-------Q 600

Query: 601 LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIRH 660
           LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPS EKLASSHLPAAFDDVIHVIRH
Sbjct: 601 LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSFEKLASSHLPAAFDDVIHVIRH 660

Query: 661 SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS 720
           SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS
Sbjct: 661 SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS 720

Query: 721 FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN 780
           FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN
Sbjct: 721 FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN 777

Query: 781 ARSS 784
           ARSS
Sbjct: 781 ARSS 777

BLAST of Csor.00g222420 vs. NCBI nr
Match: XP_022995026.1 (serine/threonine-protein kinase Nek5-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1469 bits (3802), Expect = 0.0
Identity = 756/790 (95.70%), Postives = 765/790 (96.84%), Query Frame = 0

Query: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDHYEIMEQIGRGAFGAAILV+HKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDHYEIMEQIGRGAFGAAILVYHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMA+LMKKSNG+HFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGLHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHS+FVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSI P VD  PDKRR TRDSRR MAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSITP-VDSLPDKRRTTRDSRRSMAESQNS 300

Query: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360
           NSSNSDRDSLISSDKNAPAMASNCDDRVTDND ASVDDHDGPELLVPSEEEQTHNTCSLK
Sbjct: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDKASVDDHDGPELLVPSEEEQTHNTCSLK 360

Query: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA 420
           VNDPMHSKPAQADHQS+TESRRPKTIRNIMLALKEGKARENYSPV G CVKSGVASN KA
Sbjct: 361 VNDPMHSKPAQADHQSHTESRRPKTIRNIMLALKEGKARENYSPVPGICVKSGVASNQKA 420

Query: 421 HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480
           HAE+PPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLP+VDPT KTK
Sbjct: 421 HAEVPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPMVDPTPKTK 480

Query: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH 540
            RYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPAR RQAE+NVPDAVSSTE IH
Sbjct: 481 QRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARTRQAEINVPDAVSSTERIH 540

Query: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE 600
           QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE
Sbjct: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE 600

Query: 601 LEETHPPSCSSSLQYEKGNPG-VDDKP-----LSDKVTSLNNPSSEKLASSHLPAAFDDV 660
           LEETHPPSCSSSLQYEKGNPG VDDKP     LSD+VTSLNNP SEKLASSHLPAAFDDV
Sbjct: 601 LEETHPPSCSSSLQYEKGNPGGVDDKPTHECSLSDEVTSLNNPPSEKLASSHLPAAFDDV 660

Query: 661 IHVIRHSSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARE 720
           IHVIRHSSFRVGSEQA TENLED SS KEMDGGSPPT PTKNSDSSEHTKPVEEEVAARE
Sbjct: 661 IHVIRHSSFRVGSEQALTENLEDHSSDKEMDGGSPPTVPTKNSDSSEHTKPVEEEVAARE 720

Query: 721 TLDVKSFRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKG 780
           TLDVKSFRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKG
Sbjct: 721 TLDVKSFRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKG 780

Query: 781 MKMEDNARSS 784
           MKMEDNARSS
Sbjct: 781 MKMEDNARSS 789

BLAST of Csor.00g222420 vs. ExPASy TrEMBL
Match: A0A6J1GZX8 (serine/threonine-protein kinase Nek5-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459186 PE=4 SV=1)

HSP 1 Score: 1540 bits (3988), Expect = 0.0
Identity = 782/784 (99.74%), Postives = 783/784 (99.87%), Query Frame = 0

Query: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHS+FVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRR MAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRSMAESQNS 300

Query: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360
           NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK
Sbjct: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360

Query: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA 420
           VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA
Sbjct: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA 420

Query: 421 HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480
           HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK
Sbjct: 421 HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480

Query: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH 540
           PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH
Sbjct: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH 540

Query: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE 600
           QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE
Sbjct: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE 600

Query: 601 LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIRH 660
           LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIRH
Sbjct: 601 LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIRH 660

Query: 661 SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS 720
           SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS
Sbjct: 661 SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS 720

Query: 721 FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN 780
           FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN
Sbjct: 721 FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKGMKMEDN 780

Query: 781 ARSS 784
           ARSS
Sbjct: 781 ARSS 784

BLAST of Csor.00g222420 vs. ExPASy TrEMBL
Match: A0A6J1K0V2 (serine/threonine-protein kinase Nek5-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490704 PE=4 SV=1)

HSP 1 Score: 1469 bits (3802), Expect = 0.0
Identity = 756/790 (95.70%), Postives = 765/790 (96.84%), Query Frame = 0

Query: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDHYEIMEQIGRGAFGAAILV+HKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDHYEIMEQIGRGAFGAAILVYHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMA+LMKKSNG+HFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGLHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHS+FVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSI P VD  PDKRR TRDSRR MAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSITP-VDSLPDKRRTTRDSRRSMAESQNS 300

Query: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360
           NSSNSDRDSLISSDKNAPAMASNCDDRVTDND ASVDDHDGPELLVPSEEEQTHNTCSLK
Sbjct: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDKASVDDHDGPELLVPSEEEQTHNTCSLK 360

Query: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA 420
           VNDPMHSKPAQADHQS+TESRRPKTIRNIMLALKEGKARENYSPV G CVKSGVASN KA
Sbjct: 361 VNDPMHSKPAQADHQSHTESRRPKTIRNIMLALKEGKARENYSPVPGICVKSGVASNQKA 420

Query: 421 HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480
           HAE+PPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLP+VDPT KTK
Sbjct: 421 HAEVPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPMVDPTPKTK 480

Query: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH 540
            RYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPAR RQAE+NVPDAVSSTE IH
Sbjct: 481 QRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARTRQAEINVPDAVSSTERIH 540

Query: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE 600
           QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE
Sbjct: 541 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE 600

Query: 601 LEETHPPSCSSSLQYEKGNPG-VDDKP-----LSDKVTSLNNPSSEKLASSHLPAAFDDV 660
           LEETHPPSCSSSLQYEKGNPG VDDKP     LSD+VTSLNNP SEKLASSHLPAAFDDV
Sbjct: 601 LEETHPPSCSSSLQYEKGNPGGVDDKPTHECSLSDEVTSLNNPPSEKLASSHLPAAFDDV 660

Query: 661 IHVIRHSSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARE 720
           IHVIRHSSFRVGSEQA TENLED SS KEMDGGSPPT PTKNSDSSEHTKPVEEEVAARE
Sbjct: 661 IHVIRHSSFRVGSEQALTENLEDHSSDKEMDGGSPPTVPTKNSDSSEHTKPVEEEVAARE 720

Query: 721 TLDVKSFRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKG 780
           TLDVKSFRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKG
Sbjct: 721 TLDVKSFRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLKG 780

Query: 781 MKMEDNARSS 784
           MKMEDNARSS
Sbjct: 781 MKMEDNARSS 789

BLAST of Csor.00g222420 vs. ExPASy TrEMBL
Match: A0A6J1H131 (serine/threonine-protein kinase Nek5-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459186 PE=4 SV=1)

HSP 1 Score: 1348 bits (3488), Expect = 0.0
Identity = 686/688 (99.71%), Postives = 687/688 (99.85%), Query Frame = 0

Query: 97  MKKSNGMHFPEEKLCKWFTQLLLAVEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAK 156
           MKKSNGMHFPEEKLCKWFTQLLLAVEYLHS+FVLHRDLKCSNIFLTKDRDVRLGDFGLAK
Sbjct: 1   MKKSNGMHFPEEKLCKWFTQLLLAVEYLHSNFVLHRDLKCSNIFLTKDRDVRLGDFGLAK 60

Query: 157 TLKADDLASSVVGTPNYMCPELLADIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLI 216
           TLKADDLASSVVGTPNYMCPELLADIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLI
Sbjct: 61  TLKADDLASSVVGTPNYMCPELLADIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLI 120

Query: 217 SKINRSSIGPLPSCYSPSLKTLIKGMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPT 276
           SKINRSSIGPLPSCYSPSLKTLIKGMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPT
Sbjct: 121 SKINRSSIGPLPSCYSPSLKTLIKGMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPT 180

Query: 277 VDFSPDKRRPTRDSRRCMAESQNSNSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASV 336
           VDFSPDKRRPTRDSRR MAESQNSNSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASV
Sbjct: 181 VDFSPDKRRPTRDSRRSMAESQNSNSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASV 240

Query: 337 DDHDGPELLVPSEEEQTHNTCSLKVNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEG 396
           DDHDGPELLVPSEEEQTHNTCSLKVNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEG
Sbjct: 241 DDHDGPELLVPSEEEQTHNTCSLKVNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEG 300

Query: 397 KARENYSPVRGNCVKSGVASNHKAHAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIES 456
           KARENYSPVRGNCVKSGVASNHKAHAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIES
Sbjct: 301 KARENYSPVRGNCVKSGVASNHKAHAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIES 360

Query: 457 SRRIQAVATPKHQLPVVDPTSKTKPRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRP 516
           SRRIQAVATPKHQLPVVDPTSKTKPRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRP
Sbjct: 361 SRRIQAVATPKHQLPVVDPTSKTKPRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRP 420

Query: 517 TFPARARQAELNVPDAVSSTEMIHQKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEIC 576
           TFPARARQAELNVPDAVSSTEMIHQKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEIC
Sbjct: 421 TFPARARQAELNVPDAVSSTEMIHQKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEIC 480

Query: 577 DDAATPFTDIPELVLDHQSIEHDELEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNN 636
           DDAATPFTDIPELVLDHQSIEHDELEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNN
Sbjct: 481 DDAATPFTDIPELVLDHQSIEHDELEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNN 540

Query: 637 PSSEKLASSHLPAAFDDVIHVIRHSSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKN 696
           PSSEKLASSHLPAAFDDVIHVIRHSSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKN
Sbjct: 541 PSSEKLASSHLPAAFDDVIHVIRHSSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKN 600

Query: 697 SDSSEHTKPVEEEVAARETLDVKSFRQRAEALEELLELSAELLQHNRLEELGVVLKPFGK 756
           SDSSEHTKPVEEEVAARETLDVKSFRQRAEALEELLELSAELLQHNRLEELGVVLKPFGK
Sbjct: 601 SDSSEHTKPVEEEVAARETLDVKSFRQRAEALEELLELSAELLQHNRLEELGVVLKPFGK 660

Query: 757 DKVSPRETAIWLAKSLKGMKMEDNARSS 784
           DKVSPRETAIWLAKSLKGMKMEDNARSS
Sbjct: 661 DKVSPRETAIWLAKSLKGMKMEDNARSS 688

BLAST of Csor.00g222420 vs. ExPASy TrEMBL
Match: A0A6J1JXL4 (serine/threonine-protein kinase Nek5-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490704 PE=4 SV=1)

HSP 1 Score: 1280 bits (3312), Expect = 0.0
Identity = 662/694 (95.39%), Postives = 669/694 (96.40%), Query Frame = 0

Query: 97  MKKSNGMHFPEEKLCKWFTQLLLAVEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAK 156
           MKKSNG+HFPEEKLCKWFTQLLLAVEYLHS+FVLHRDLKCSNIFLTKDRDVRLGDFGLAK
Sbjct: 1   MKKSNGLHFPEEKLCKWFTQLLLAVEYLHSNFVLHRDLKCSNIFLTKDRDVRLGDFGLAK 60

Query: 157 TLKADDLASSVVGTPNYMCPELLADIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLI 216
           TLKADDLASSVVGTPNYMCPELLADIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLI
Sbjct: 61  TLKADDLASSVVGTPNYMCPELLADIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLI 120

Query: 217 SKINRSSIGPLPSCYSPSLKTLIKGMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPT 276
           SKINRSSIGPLPSCYSPSLKTLIKGMLRKNPEHRPNASELLKHPYLQPYVEQYRPSI P 
Sbjct: 121 SKINRSSIGPLPSCYSPSLKTLIKGMLRKNPEHRPNASELLKHPYLQPYVEQYRPSITP- 180

Query: 277 VDFSPDKRRPTRDSRRCMAESQNSNSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASV 336
           VD  PDKRR TRDSRR MAESQNSNSSNSDRDSLISSDKNAPAMASNCDDRVTDND ASV
Sbjct: 181 VDSLPDKRRTTRDSRRSMAESQNSNSSNSDRDSLISSDKNAPAMASNCDDRVTDNDKASV 240

Query: 337 DDHDGPELLVPSEEEQTHNTCSLKVNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEG 396
           DDHDGPELLVPSEEEQTHNTCSLKVNDPMHSKPAQADHQS+TESRRPKTIRNIMLALKEG
Sbjct: 241 DDHDGPELLVPSEEEQTHNTCSLKVNDPMHSKPAQADHQSHTESRRPKTIRNIMLALKEG 300

Query: 397 KARENYSPVRGNCVKSGVASNHKAHAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIES 456
           KARENYSPV G CVKSGVASN KAHAE+PPKVIKPPVAVPGSKSNADTPTTVPAKANIES
Sbjct: 301 KARENYSPVPGICVKSGVASNQKAHAEVPPKVIKPPVAVPGSKSNADTPTTVPAKANIES 360

Query: 457 SRRIQAVATPKHQLPVVDPTSKTKPRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRP 516
           SRRIQAVATPKHQLP+VDPT KTK RYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRP
Sbjct: 361 SRRIQAVATPKHQLPMVDPTPKTKQRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRP 420

Query: 517 TFPARARQAELNVPDAVSSTEMIHQKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEIC 576
           TFPAR RQAE+NVPDAVSSTE IHQKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEIC
Sbjct: 421 TFPARTRQAEINVPDAVSSTERIHQKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEIC 480

Query: 577 DDAATPFTDIPELVLDHQSIEHDELEETHPPSCSSSLQYEKGNPG-VDDKP-----LSDK 636
           DDAATPFTDIPELVLDHQSIEHDELEETHPPSCSSSLQYEKGNPG VDDKP     LSD+
Sbjct: 481 DDAATPFTDIPELVLDHQSIEHDELEETHPPSCSSSLQYEKGNPGGVDDKPTHECSLSDE 540

Query: 637 VTSLNNPSSEKLASSHLPAAFDDVIHVIRHSSFRVGSEQAATENLEDRSSCKEMDGGSPP 696
           VTSLNNP SEKLASSHLPAAFDDVIHVIRHSSFRVGSEQA TENLED SS KEMDGGSPP
Sbjct: 541 VTSLNNPPSEKLASSHLPAAFDDVIHVIRHSSFRVGSEQALTENLEDHSSDKEMDGGSPP 600

Query: 697 TAPTKNSDSSEHTKPVEEEVAARETLDVKSFRQRAEALEELLELSAELLQHNRLEELGVV 756
           T PTKNSDSSEHTKPVEEEVAARETLDVKSFRQRAEALEELLELSAELLQHNRLEELGVV
Sbjct: 601 TVPTKNSDSSEHTKPVEEEVAARETLDVKSFRQRAEALEELLELSAELLQHNRLEELGVV 660

Query: 757 LKPFGKDKVSPRETAIWLAKSLKGMKMEDNARSS 784
           LKPFGKDKVSPRETAIWLAKSLKGMKMEDNARSS
Sbjct: 661 LKPFGKDKVSPRETAIWLAKSLKGMKMEDNARSS 693

BLAST of Csor.00g222420 vs. ExPASy TrEMBL
Match: A0A6J1FSG2 (serine/threonine-protein kinase Nek5-like OS=Cucurbita moschata OX=3662 GN=LOC111448376 PE=4 SV=1)

HSP 1 Score: 1240 bits (3208), Expect = 0.0
Identity = 682/956 (71.34%), Postives = 716/956 (74.90%), Query Frame = 0

Query: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMD YEIMEQIGRGAFGAAILV+HKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMA+LMKKSNGMHFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHS+FVLHRDLKCSNIFLTKD+DVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNFVLHRDLKCSNIFLTKDKDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAA RPAFKAFDMAGLISKINRSS+GPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSMGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSI+P+VDF  DK R  RDSRR MAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSISPSVDFPSDKPRAARDSRRSMAESQNS 300

Query: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLK 360
           NSSNSDRDSL SSDKN PA ASNCDD+VT +DMASVDDHDG E L+P E+EQTHN CSLK
Sbjct: 301 NSSNSDRDSLFSSDKNTPATASNCDDKVTVSDMASVDDHDGSETLLPGEDEQTHNMCSLK 360

Query: 361 VNDPMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKA 420
           VNDPMHSK  Q DHQSNTESRRPKTIRNIMLALKEGKAREN+SPVRGN VKSGVASN KA
Sbjct: 361 VNDPMHSKTNQEDHQSNTESRRPKTIRNIMLALKEGKARENFSPVRGNRVKSGVASNQKA 420

Query: 421 HAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTK 480
           HAE+PPK IKPPVA PG KSN DT  TVP KANIESSRR+Q VATPKHQ+PVV PT KTK
Sbjct: 421 HAEVPPKFIKPPVAEPGFKSNPDTHITVPTKANIESSRRVQVVATPKHQVPVVVPTPKTK 480

Query: 481 PRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIH 540
            R+DN PPL  P+KPV+DGLSSKSRQRTPPT+LRRP+FPAR RQ    VPDAVSS E IH
Sbjct: 481 TRHDNSPPLCRPLKPVEDGLSSKSRQRTPPTLLRRPSFPARMRQVGQAVPDAVSSMERIH 540

Query: 541 QKET----------------------QHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDD 600
           QKET                      QH +SE  SKGMQTDSSNSVSSSVSIQGFEICDD
Sbjct: 541 QKETSPEEVQSSHQDHVVSMELTKETQHASSEHCSKGMQTDSSNSVSSSVSIQGFEICDD 600

Query: 601 AATPFTD-IPELVLDHQSIEHDELEETHPPSCSSSLQYE---------KGNPGVDDKP-- 660
           AATPF D + + V DHQSIEHDE+EETHPPSCS SLQYE         KGN GV DKP  
Sbjct: 601 AATPFADGVIDPVFDHQSIEHDEIEETHPPSCSPSLQYEMSEHNLLTDKGNHGVHDKPTE 660

Query: 661 ------------------------------------------------------------ 720
                                                                       
Sbjct: 661 CSLEAGVEKESPSVELDLPLKSEGRFVFKDDIPINIPSNRPNMVTEPNTISSHGDDKFTV 720

Query: 721 ---------------------------LSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIR 780
                                      L DKVT+L+NPS+EKLAS+HLPAAFDDVIHVIR
Sbjct: 721 RELLSSVAEPTATPLVATPSSSTHKCLLPDKVTTLHNPSAEKLASNHLPAAFDDVIHVIR 780

Query: 781 HSSFRVGSEQAATENLE------------------------------------------- 784
           HSSFRVGSEQ+ TENLE                                           
Sbjct: 781 HSSFRVGSEQSVTENLEVGVQNVDVGKLVTVVKDELELKNGTTTPLTLKSSSCSDAMSLK 840

BLAST of Csor.00g222420 vs. TAIR 10
Match: AT3G44200.1 (NIMA (never in mitosis, gene A)-related 6 )

HSP 1 Score: 780.8 bits (2015), Expect = 1.0e-225
Identity = 486/946 (51.37%), Postives = 582/946 (61.52%), Query Frame = 0

Query: 1   MESRMDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMD YE+MEQIGRGAFGAAILVHHK+E+KKYVLKKIRLARQTERCRRSAHQEM+LIA
Sbjct: 1   MESRMDQYELMEQIGRGAFGAAILVHHKAERKKYVLKKIRLARQTERCRRSAHQEMSLIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMA+LMKKSNG++FPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGVYFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHS++VLHRDLKCSNIFLTKD+DVRLGDFGLAKTLKADDL SSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNYVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLTSSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCC+YEMAA RPAFKAFDMAGLISK+NRSSIGPLP CYSPSLK LIK
Sbjct: 181 DIPYGFKSDIWSLGCCIYEMAAYRPAFKAFDMAGLISKVNRSSIGPLPPCYSPSLKALIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNS 300
           GMLRKNPE+RPNASE+LKHPYLQPYVEQYRP+++     +P+K   +R+ RR MAESQNS
Sbjct: 241 GMLRKNPEYRPNASEILKHPYLQPYVEQYRPTLS-AASITPEKPLNSREGRRSMAESQNS 300

Query: 301 NSSNSDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEE-QTHNTCSL 360
           NSS S++D+   SDKN   +  +  ++VT+ D   VDD D  + +  S E     +  + 
Sbjct: 301 NSS-SEKDNFYVSDKNIRYVVPSNGNKVTETDSGFVDDEDILDHVQQSAENGNLQSVSAT 360

Query: 361 KVNDPMHSKPAQADHQSNT-ESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNH 420
           K +     KP  +D + +  + R PKTIRNIM+ LKE KAREN SP+R N  +       
Sbjct: 361 KPDGHGILKPVHSDQRPDVIQPRHPKTIRNIMMVLKEEKARENGSPMRSNRSRPSSVPTQ 420

Query: 421 KAHAEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSK 480
           K + E P K+ K       SK+NA TP  +P       S R      PKH +PV+D + K
Sbjct: 421 KNNVETPSKIPKLGDIAHSSKTNASTP--IPPSKLASDSARTPGSFPPKHHMPVIDSSPK 480

Query: 481 TKPRYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPA-RARQAELNVPD-AVSST 540
            KPR D   P        ++ +S K RQRTPPT+ RR +  A ++RQ   ++ + A   T
Sbjct: 481 LKPRNDRISPSPAAKHEAEEAMSVKRRQRTPPTLPRRTSLIAHQSRQLGADISNMAAKET 540

Query: 541 EMIH-----QKETQ-----------HTTSEP------FSKGMQTDSSNSVSSSVSIQGFE 600
             +H     + ET             TT EP       +KGMQ++SSNS+SSS+S+Q FE
Sbjct: 541 AKLHPSVPSESETNSHQSRVHASPVSTTPEPKRTSVGSAKGMQSESSNSISSSLSMQAFE 600

Query: 601 ICDDAATPFTDIPELVL--DHQSIEHDELEETHPP------------------------- 660
           +CDDA+TP+ D+ E     DH+   H E   + P                          
Sbjct: 601 LCDDASTPYIDMTEHTTPDDHRRSCHSEYSYSFPDISSEMMIRRDEHSTSMRLTEIPDSV 660

Query: 661 -----------------SCSSSL----------------QYEKGN------------PGV 720
                            SC + L                Q++ G+            PG 
Sbjct: 661 SGVQNTIAHHQPEREQGSCPTVLKDDSPATLQSYEPNTSQHQHGDDKFTVKEFVSSVPGP 720

Query: 721 DDKPL-----------SDKVTSL---NNPSSEKLASSHLPAAFDDVIHVIRHSSFRVGSE 780
              PL           SD  TS+   N+   +  + SH     DDVIHVIRHSSFRVGS+
Sbjct: 721 APLPLHVEPSHQVNSHSDNKTSVMSQNSALEKNNSHSHPHPVVDDVIHVIRHSSFRVGSD 780

BLAST of Csor.00g222420 vs. TAIR 10
Match: AT3G04810.1 (NIMA-related kinase 2 )

HSP 1 Score: 427.9 bits (1099), Expect = 1.7e-119
Identity = 300/773 (38.81%), Postives = 396/773 (51.23%), Query Frame = 0

Query: 5   MDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIARVQH 64
           M++YE++EQIG+G+FG+A+LV HK EKK YVLKKIRLARQT R RRSAHQEM LI+++ +
Sbjct: 1   MENYEVLEQIGKGSFGSALLVRHKHEKKLYVLKKIRLARQTGRTRRSAHQEMELISKIHN 60

Query: 65  PYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLAVEYL 124
           P+IVE+K++WVEKGCYVCI+ GYC+GGDMA+ +KK+NG+HF EEKLCKW  Q+LLA+EYL
Sbjct: 61  PFIVEYKDSWVEKGCYVCIIIGYCKGGDMAEAIKKTNGVHFTEEKLCKWLVQILLALEYL 120

Query: 125 HSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLADIPY 184
           H++ +LHRD+KCSNIFLTKD+D+RLGDFGLAK L +DDLASSVVGTP+YMCPELLADIPY
Sbjct: 121 HANHILHRDVKCSNIFLTKDQDIRLGDFGLAKVLTSDDLASSVVGTPSYMCPELLADIPY 180

Query: 185 GFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIKGMLR 244
           G KSDIWSLGCCMYEM A +PAFKAFDM GLI++INRS + PLP+ YS + + L+K MLR
Sbjct: 181 GSKSDIWSLGCCMYEMTAMKPAFKAFDMQGLINRINRSIVPPLPAQYSAAFRGLVKSMLR 240

Query: 245 KNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNSNSSN 304
           KNPE RP+A+ELL+ P LQPY+++                                    
Sbjct: 241 KNPELRPSAAELLRQPLLQPYIQKIH---------------------------------- 300

Query: 305 SDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLKVNDP 364
                                                                 LKVNDP
Sbjct: 301 ------------------------------------------------------LKVNDP 360

Query: 365 -MHSKPAQ-ADHQSNTESRRPKTIRNIMLALKEGKARE-NYSPVRGNCVKSGVASNHKAH 424
             +  PAQ  + +S   +  P+  R        GK+     S  RGN ++  V+S     
Sbjct: 361 GSNVLPAQWPESESARRNSFPEQRRR-----PAGKSHSFGPSRFRGN-LEDSVSS----- 420

Query: 425 AEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTKP 484
                 + K   A    +   D  T       +   RR    +     +PV    S  +P
Sbjct: 421 ------IKKTVPAYLNRERQVDLSTDASGDGTV--VRRTSEASKSSRYVPVRASASPVRP 480

Query: 485 RYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQA-ELNVPDAVSSTEMIH 544
           R         PV       SS+ + R P  ++RR + P+  + A E+     +S T  +H
Sbjct: 481 RQPRSDLGQLPV-------SSQLKNRKPAALIRRASMPSSRKPAKEIKDSLYISKTSFLH 540

Query: 545 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE 604
           Q  +              D S +      I+ F +      PF  +   V   +      
Sbjct: 541 QINS-------------PDVSMNAPRIDKIE-FPLASYEEEPFVPV---VRGKKKKASSR 597

Query: 605 LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIRH 664
              + PP            P +D     DK T     + E    + + A +++  +    
Sbjct: 601 GSYSPPP-----------EPPLDCSITKDKFTLEPGQNRE---GAIMKAVYEEDAY---- 597

Query: 665 SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS 724
                         LEDRS   + +     TA   +  SS            R+  D  S
Sbjct: 661 --------------LEDRSESSDQNA----TAGASSRASSG---------VRRQRFDPSS 597

Query: 725 FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLK 774
           ++QRAEALE LLE SA LLQ  R +EL V+L+PFG  KVSPRETAIWL+KS K
Sbjct: 721 YQQRAEALEGLLEFSARLLQDERYDELNVLLRPFGPGKVSPRETAIWLSKSFK 597

BLAST of Csor.00g222420 vs. TAIR 10
Match: AT3G04810.2 (NIMA-related kinase 2 )

HSP 1 Score: 427.9 bits (1099), Expect = 1.7e-119
Identity = 300/773 (38.81%), Postives = 396/773 (51.23%), Query Frame = 0

Query: 5   MDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIARVQH 64
           M++YE++EQIG+G+FG+A+LV HK EKK YVLKKIRLARQT R RRSAHQEM LI+++ +
Sbjct: 1   MENYEVLEQIGKGSFGSALLVRHKHEKKLYVLKKIRLARQTGRTRRSAHQEMELISKIHN 60

Query: 65  PYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLAVEYL 124
           P+IVE+K++WVEKGCYVCI+ GYC+GGDMA+ +KK+NG+HF EEKLCKW  Q+LLA+EYL
Sbjct: 61  PFIVEYKDSWVEKGCYVCIIIGYCKGGDMAEAIKKTNGVHFTEEKLCKWLVQILLALEYL 120

Query: 125 HSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLADIPY 184
           H++ +LHRD+KCSNIFLTKD+D+RLGDFGLAK L +DDLASSVVGTP+YMCPELLADIPY
Sbjct: 121 HANHILHRDVKCSNIFLTKDQDIRLGDFGLAKVLTSDDLASSVVGTPSYMCPELLADIPY 180

Query: 185 GFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIKGMLR 244
           G KSDIWSLGCCMYEM A +PAFKAFDM GLI++INRS + PLP+ YS + + L+K MLR
Sbjct: 181 GSKSDIWSLGCCMYEMTAMKPAFKAFDMQGLINRINRSIVPPLPAQYSAAFRGLVKSMLR 240

Query: 245 KNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNSNSSN 304
           KNPE RP+A+ELL+ P LQPY+++                                    
Sbjct: 241 KNPELRPSAAELLRQPLLQPYIQKIH---------------------------------- 300

Query: 305 SDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLKVNDP 364
                                                                 LKVNDP
Sbjct: 301 ------------------------------------------------------LKVNDP 360

Query: 365 -MHSKPAQ-ADHQSNTESRRPKTIRNIMLALKEGKARE-NYSPVRGNCVKSGVASNHKAH 424
             +  PAQ  + +S   +  P+  R        GK+     S  RGN ++  V+S     
Sbjct: 361 GSNVLPAQWPESESARRNSFPEQRRR-----PAGKSHSFGPSRFRGN-LEDSVSS----- 420

Query: 425 AEMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTKP 484
                 + K   A    +   D  T       +   RR    +     +PV    S  +P
Sbjct: 421 ------IKKTVPAYLNRERQVDLSTDASGDGTV--VRRTSEASKSSRYVPVRASASPVRP 480

Query: 485 RYDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQA-ELNVPDAVSSTEMIH 544
           R         PV       SS+ + R P  ++RR + P+  + A E+     +S T  +H
Sbjct: 481 RQPRSDLGQLPV-------SSQLKNRKPAALIRRASMPSSRKPAKEIKDSLYISKTSFLH 540

Query: 545 QKETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDE 604
           Q  +              D S +      I+ F +      PF  +   V   +      
Sbjct: 541 QINS-------------PDVSMNAPRIDKIE-FPLASYEEEPFVPV---VRGKKKKASSR 597

Query: 605 LEETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIRH 664
              + PP            P +D     DK T     + E    + + A +++  +    
Sbjct: 601 GSYSPPP-----------EPPLDCSITKDKFTLEPGQNRE---GAIMKAVYEEDAY---- 597

Query: 665 SSFRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKS 724
                         LEDRS   + +     TA   +  SS            R+  D  S
Sbjct: 661 --------------LEDRSESSDQNA----TAGASSRASSG---------VRRQRFDPSS 597

Query: 725 FRQRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLK 774
           ++QRAEALE LLE SA LLQ  R +EL V+L+PFG  KVSPRETAIWL+KS K
Sbjct: 721 YQQRAEALEGLLEFSARLLQDERYDELNVLLRPFGPGKVSPRETAIWLSKSFK 597

BLAST of Csor.00g222420 vs. TAIR 10
Match: AT5G28290.1 (NIMA-related kinase 3 )

HSP 1 Score: 427.2 bits (1097), Expect = 2.9e-119
Identity = 305/770 (39.61%), Postives = 380/770 (49.35%), Query Frame = 0

Query: 5   MDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIARVQH 64
           M+HYE++EQIG+G+FG+A+LV HK EKK YVLKKIRLARQT R RRSAHQEM LI+++++
Sbjct: 1   MEHYEVLEQIGKGSFGSALLVRHKHEKKLYVLKKIRLARQTGRTRRSAHQEMELISKIRN 60

Query: 65  PYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLAVEYL 124
           P+IVE+K++WVEKGCYVCIV GYC+GGDMA+ +KK+NG+ F EEKLCKW  QLL+A+EYL
Sbjct: 61  PFIVEYKDSWVEKGCYVCIVIGYCKGGDMAEAIKKANGVEFSEEKLCKWLVQLLMALEYL 120

Query: 125 HSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLADIPY 184
           H+S +LHRD+KCSNIFLTKD+D+RLGDFGLAK L +DDLASSVVGTP+YMCPELLADIPY
Sbjct: 121 HASHILHRDVKCSNIFLTKDQDIRLGDFGLAKILTSDDLASSVVGTPSYMCPELLADIPY 180

Query: 185 GFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIKGMLR 244
           G KSDIWSLGCCMYEM A +PAFKAFDM GLI++INRS + PLP+ YS + ++L+K MLR
Sbjct: 181 GSKSDIWSLGCCMYEMTALKPAFKAFDMQGLINRINRSIVAPLPAQYSTAFRSLVKSMLR 240

Query: 245 KNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNSNSSN 304
           KNPE RP+AS+LL+ P LQPYV++    +   + F      P+   RR     Q   +S 
Sbjct: 241 KNPELRPSASDLLRQPLLQPYVQK----VLLKLSFREHDTLPSESERRSSYPQQRKRTSG 300

Query: 305 SDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEE-QTHNTCSLKVND 364
                   S    P+                VD  D    + P       H    L  ND
Sbjct: 301 -------KSVSFGPS-------------RFGVDQEDSVSSVKPVHTYLHRHRPVDLSAND 360

Query: 365 PMHSKPAQADHQSNTESRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKAHAE 424
                       S    RRP     +  + K       Y PVR N  KSG          
Sbjct: 361 -----------TSRVVVRRPAVSSVVSNSSK-------YVPVRSNQPKSG---------- 420

Query: 425 MPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTKPRY 484
               ++KP                              AV T +  LP+       KP  
Sbjct: 421 ---GLLKP------------------------------AVVTRRASLPI-----SQKPAK 480

Query: 485 DNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIHQKE 544
                L+HP   +   L+S       P +  R  FP              S  EM     
Sbjct: 481 GTKDSLYHPNIGILHQLNSPDVSVNSPRI-DRIKFP------------LASYEEM----- 540

Query: 545 TQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDELEE 604
                  PF   ++    +S  S                ++  PE  LD           
Sbjct: 541 -------PFIPVVRKKKGSSRGS----------------YSPPPEPPLD----------- 558

Query: 605 THPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIRHSSF 664
                CS +                DK T       E+   S L                
Sbjct: 601 -----CSIT---------------KDKFTL----EPERETKSDL---------------- 558

Query: 665 RVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKSFRQ 724
              S+Q AT     R+S                              + R+  D  S+RQ
Sbjct: 661 ---SDQNATAGASSRAS---------------------------SGASRRQRFDPSSYRQ 558

Query: 725 RAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLK 774
           RAEALE LLE SA LL   R +EL V+LKPFG  KVSPRETAIWL+KS K
Sbjct: 721 RAEALEGLLEFSARLLIDERYDELNVLLKPFGPGKVSPRETAIWLSKSFK 558

BLAST of Csor.00g222420 vs. TAIR 10
Match: AT1G54510.1 (NIMA-related serine/threonine kinase 1 )

HSP 1 Score: 424.5 bits (1090), Expect = 1.9e-118
Identity = 305/771 (39.56%), Postives = 412/771 (53.44%), Query Frame = 0

Query: 5   MDHYEIMEQIGRGAFGAAILVHHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIARVQH 64
           M+ YE +EQIG+G+FG+A+LV HK EKKKYVLKKIRLARQT+R RRSAHQEM LI++++H
Sbjct: 1   MEQYEFLEQIGKGSFGSALLVRHKHEKKKYVLKKIRLARQTQRTRRSAHQEMELISKMRH 60

Query: 65  PYIVEFKEAWVEKGCYVCIVTGYCEGGDMADLMKKSNGMHFPEEKLCKWFTQLLLAVEYL 124
           P+IVE+K++WVEK CYVCIV GYCEGGDMA  +KKSNG+HF EEKLCKW  QLL+ +EYL
Sbjct: 61  PFIVEYKDSWVEKACYVCIVIGYCEGGDMAQAIKKSNGVHFQEEKLCKWLVQLLMGLEYL 120

Query: 125 HSSFVLHRDLKCSNIFLTKDRDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLADIPY 184
           HS+ +LHRD+KCSNIFLTK++D+RLGDFGLAK L +DDL SSVVGTP+YMCPELLADIPY
Sbjct: 121 HSNHILHRDVKCSNIFLTKEQDIRLGDFGLAKILTSDDLTSSVVGTPSYMCPELLADIPY 180

Query: 185 GFKSDIWSLGCCMYEMAARRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIKGMLR 244
           G KSDIWSLGCC+YEMA  +PAFKAFDM  LI+KIN++ + PLP+ YS   + L+K MLR
Sbjct: 181 GSKSDIWSLGCCIYEMAYLKPAFKAFDMQALINKINKTIVSPLPAKYSGPFRGLVKSMLR 240

Query: 245 KNPEHRPNASELLKHPYLQPYVEQYRPSIAPTVDFSPDKRRPTRDSRRCMAESQNSNSSN 304
           KNPE RP+AS+LL+HP+LQPYV   +  +      +     P+  S+R M ++  S    
Sbjct: 241 KNPEVRPSASDLLRHPHLQPYVLDVKLRLNNLRRKTLPPELPS--SKRIMKKAHFSE--- 300

Query: 305 SDRDSLISSDKNAPAMASNCDDRVTDNDMASVDDHDGPELLVPSEEEQTHNTCSLKVNDP 364
                                                P +  P+  E+ H +     ND 
Sbjct: 301 -------------------------------------PAVTCPAFGERQHRSL---WNDR 360

Query: 365 MHSKPAQADHQSNTE--SRRPKTIRNIMLALKEGKARENYSPVRGNCVKSGVASNHKAHA 424
             +  A+ D  S+ +  SRR   I ++ +           S  +G  +   V+S+     
Sbjct: 361 ALNPEAEEDTASSIKCISRR---ISDLSIE----------SSSKGTLICKQVSSS----- 420

Query: 425 EMPPKVIKPPVAVPGSKSNADTPTTVPAKANIESSRRIQAVATPKHQLPVVDPTSKTKPR 484
               KV K P+                AK+++ +SRRI                 +T  R
Sbjct: 421 --ACKVSKYPL----------------AKSSV-TSRRIM----------------ETGRR 480

Query: 485 YDNYPPLFHPVKPVDDGLSSKSRQRTPPTVLRRPTFPARARQAELNVPDAVSSTEMIHQK 544
            D+     HPV     G +SK                         +P A          
Sbjct: 481 SDH----LHPVS--GGGTTSKI------------------------IPSA---------- 540

Query: 545 ETQHTTSEPFSKGMQTDSSNSVSSSVSIQGFEICDDAATPFTDIPELVLDHQSIEHDELE 604
                TS P +K     ++  V++   I G  I  +  +P   I E  +D  +I      
Sbjct: 541 ---RRTSLPLTK---RATNQEVAAYNPIVG--ILQNVKSPEYSINEPQVDKIAI------ 589

Query: 605 ETHPPSCSSSLQYEKGNPGVDDKPLSDKVTSLNNPSSEKLASSHLPAAFDDVIHVIRHSS 664
                       YE+    +   P+  K +S ++  S++  +        D   V  H++
Sbjct: 601 -------FPLAPYEQ---DIFFTPMQRKTSSKSSSVSDRSITK-------DKCTVQTHTT 589

Query: 665 FRVGSEQAATENLEDRSSCKEMDGGSPPTAPTKNSDSSEHTKPVEEEVAARETLDVKSFR 724
           ++ G +    +N+ D SS  + +  +  ++ T +S S             R   D  S+R
Sbjct: 661 WQ-GIQLNMVDNISDGSSSSDQNATAGASSHTTSSSS------------RRCRFDPSSYR 589

Query: 725 QRAEALEELLELSAELLQHNRLEELGVVLKPFGKDKVSPRETAIWLAKSLK 774
           QRA+ALE LLE SA LLQ  R +EL V+LKPFG  KVSPRETAIW+AKSLK
Sbjct: 721 QRADALEGLLEFSARLLQEGRYDELNVLLKPFGPGKVSPRETAIWIAKSLK 589