Csor.00g215520 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g215520
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionVacuolar-processing enzyme
LocationCsor_Chr10: 2989895 .. 2991836 (+)
RNA-Seq ExpressionCsor.00g215520
SyntenyCsor.00g215520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAACTTTTGCTACAACTCCAACCACACTCCTCCTCATAGCTCTTCTGTTTTTCAACAGCATTATTGCTAAGAAACAGTGGGTCAGTCATGCCAATACCGACCAACCCGGCAAGCGATGGGCACTCCTTGTTGCTGGCTCCAATGGTTATGACAATTACCGACATCAGGTGCTCTTTATATCAATACATTATTCAATAAAGTTGTCACAGTGGGACCTATGTATATAGCTAATGTATTAACTGACTATCTTCTTGACTTAGGCGGATGTATGTCATGCATATCAAATACTGAGGAAGGGTATCCCTGATGAGAACATAATCGTTTTCATGTACGACGATATTGCGTTACATCCAGACAATCCAAGGCCAGGAATCATCATCAACAAACCAGGTGGGGCTGATGTTTACCAAGGAGTTCCCAAGGACTATACAGGAAAGGATGTGAATTCAGTCAACTTTTATGCTGCCATTTTGGGGAACAAATCTGGTGTTACTGGAGGAAGTGGTAAGGTTATTGATAGTGGGCCTAATGATCATATTTTCATCTACTACACCGACCATGGTGCTGCTGGAATGCTCGGTAATAACTTACATTTTCAACTCGTTTTTGTGTCAACAAACACTAAACTCCCCTCATGAATTTGACCATAAATTGCTTCACATGATCATAGGAATGCCCATGGGTGACTATATTTACTCCAACGACCTAATGGACGTGCTGAAAAGGAAACATGAAGCCAAGAGTTACAAAAGCATGGTGATATATGTGGAGGCATGCGAGGCCGGGAGTATGTTCGAAGGATTGCTTCCAGAAAACATGAATATATATGCAACAACGGCATCAAATGCAACAGAGGATAGCTGGGCTGCATATTGTCCTGGACAGTCTCCTACTCCTCATACAGGCTATGATACTTGTCTCGGAGATTTGTACAGCGTCGCTTGGATGGAAGACAGGTAACCCGTCGGCTTAAACTTTTGAGTTCAATGGTGTTTTAACACGGTACTTAATGAATTTGTGTTGTGTCGTGTCGTGACATGCAGTGACAAGCATGATTCGAGCAAGGAAACTCTGAACCAACAATATCATACGGTAAGCTTATTGATTCTTAAGTAAGATCAGCTTAAAAAAGACAATATCTGCTAGCGGTGGGCTTGGACTGTTACACTACTGATAAACATTTTCTCGTGTATATTCCTAGGTTCGAAGAAGAACTTCGGTTGATAAAGTTGGGTACGGATCTCATGTAATGTTGTATGGAAACGAAAGCATTGGAAACAATTTCCTCAACACCTATTTTGGTAATACCTCTGATGACAACAACAATGAATCCTCTGTTAAGAGTACTCATACCATTTCTTCACTATCAAATGGGGTAAGCCAGCGTGATGCTAGCCTCCTTCATTACTGGTACAAGGTATGAGTTGTTAAACAATGGATATACATTCTAATTACGATGGCAATCAACGTAGCCTTAACTTATAGGGCTATGCTTTGATATCAGTTTCAAAATGCCCCATATGGGTCTAGAGAAAAGATGGAGGCTAGCGAGCAGCTAGAGGATGAAATTCTGAGCAGAAGGCATGCTGACAACAGTATAAATCAAATTGGTGAGCTTTTGTTTGGAGAAGCAAAGAGCTCACGAGTGCTTAACAGTATTAGGGGACATGGACAATCCTTCGTTGATGATTGGAACTGCTTTAAGAAATATGTAAGCCAATTTTCAAGTCCTGTGCTTAGCTACACATACTTTATGCTATCAAAAACTCTGCTGATGGAGTCTCTAAAACACAGGTGAAAATCTATGAGAAACATTGCAAAAGAATGTCAAGATATGGGATGAAATACACGCAAGCTCTAGCTAATATATGCAATGCTGGGATTACAATGGACCAAATGGATCAGGCTTGTCTCCAAACTTGCGTTGGTAACACCTGA

mRNA sequence

ATGACAACTTTTGCTACAACTCCAACCACACTCCTCCTCATAGCTCTTCTGTTTTTCAACAGCATTATTGCTAAGAAACAGTGGGTCAGTCATGCCAATACCGACCAACCCGGCAAGCGATGGGCACTCCTTGTTGCTGGCTCCAATGGTTATGACAATTACCGACATCAGGCGGATGTATGTCATGCATATCAAATACTGAGGAAGGGTATCCCTGATGAGAACATAATCGTTTTCATGTACGACGATATTGCGTTACATCCAGACAATCCAAGGCCAGGAATCATCATCAACAAACCAGGTGGGGCTGATGTTTACCAAGGAGTTCCCAAGGACTATACAGGAAAGGATGTGAATTCAGTCAACTTTTATGCTGCCATTTTGGGGAACAAATCTGGTGTTACTGGAGGAAGTGGTAAGGTTATTGATAGTGGGCCTAATGATCATATTTTCATCTACTACACCGACCATGGTGCTGCTGGAATGCTCGGAATGCCCATGGGTGACTATATTTACTCCAACGACCTAATGGACGTGCTGAAAAGGAAACATGAAGCCAAGAGTTACAAAAGCATGGTGATATATGTGGAGGCATGCGAGGCCGGGAGTATGTTCGAAGGATTGCTTCCAGAAAACATGAATATATATGCAACAACGGCATCAAATGCAACAGAGGATAGCTGGGCTGCATATTGTCCTGGACAGTCTCCTACTCCTCATACAGGCTATGATACTTGTCTCGGAGATTTGTACAGCGTCGCTTGGATGGAAGACAGCATTGGAAACAATTTCCTCAACACCTATTTTGGTAATACCTCTGATGACAACAACAATGAATCCTCTGTTAAGAGTACTCATACCATTTCTTCACTATCAAATGGGGTAAGCCAGCGTGATGCTAGCCTCCTTCATTACTGGTACAAGTTTCAAAATGCCCCATATGGGTCTAGAGAAAAGATGGAGGCTAGCGAGCAGCTAGAGGATGAAATTCTGAGCAGAAGGCATGCTGACAACAGTATAAATCAAATTGGTGAGCTTTTGTTTGGAGAAGCAAAGAGCTCACGAGTGCTTAACAGTATTAGGGGACATGGACAATCCTTCGTTGATGATTGGAACTGCTTTAAGAAATATGTGAAAATCTATGAGAAACATTGCAAAAGAATGTCAAGATATGGGATGAAATACACGCAAGCTCTAGCTAATATATGCAATGCTGGGATTACAATGGACCAAATGGATCAGGCTTGTCTCCAAACTTGCGTTGGTAACACCTGA

Coding sequence (CDS)

ATGACAACTTTTGCTACAACTCCAACCACACTCCTCCTCATAGCTCTTCTGTTTTTCAACAGCATTATTGCTAAGAAACAGTGGGTCAGTCATGCCAATACCGACCAACCCGGCAAGCGATGGGCACTCCTTGTTGCTGGCTCCAATGGTTATGACAATTACCGACATCAGGCGGATGTATGTCATGCATATCAAATACTGAGGAAGGGTATCCCTGATGAGAACATAATCGTTTTCATGTACGACGATATTGCGTTACATCCAGACAATCCAAGGCCAGGAATCATCATCAACAAACCAGGTGGGGCTGATGTTTACCAAGGAGTTCCCAAGGACTATACAGGAAAGGATGTGAATTCAGTCAACTTTTATGCTGCCATTTTGGGGAACAAATCTGGTGTTACTGGAGGAAGTGGTAAGGTTATTGATAGTGGGCCTAATGATCATATTTTCATCTACTACACCGACCATGGTGCTGCTGGAATGCTCGGAATGCCCATGGGTGACTATATTTACTCCAACGACCTAATGGACGTGCTGAAAAGGAAACATGAAGCCAAGAGTTACAAAAGCATGGTGATATATGTGGAGGCATGCGAGGCCGGGAGTATGTTCGAAGGATTGCTTCCAGAAAACATGAATATATATGCAACAACGGCATCAAATGCAACAGAGGATAGCTGGGCTGCATATTGTCCTGGACAGTCTCCTACTCCTCATACAGGCTATGATACTTGTCTCGGAGATTTGTACAGCGTCGCTTGGATGGAAGACAGCATTGGAAACAATTTCCTCAACACCTATTTTGGTAATACCTCTGATGACAACAACAATGAATCCTCTGTTAAGAGTACTCATACCATTTCTTCACTATCAAATGGGGTAAGCCAGCGTGATGCTAGCCTCCTTCATTACTGGTACAAGTTTCAAAATGCCCCATATGGGTCTAGAGAAAAGATGGAGGCTAGCGAGCAGCTAGAGGATGAAATTCTGAGCAGAAGGCATGCTGACAACAGTATAAATCAAATTGGTGAGCTTTTGTTTGGAGAAGCAAAGAGCTCACGAGTGCTTAACAGTATTAGGGGACATGGACAATCCTTCGTTGATGATTGGAACTGCTTTAAGAAATATGTGAAAATCTATGAGAAACATTGCAAAAGAATGTCAAGATATGGGATGAAATACACGCAAGCTCTAGCTAATATATGCAATGCTGGGATTACAATGGACCAAATGGATCAGGCTTGTCTCCAAACTTGCGTTGGTAACACCTGA

Protein sequence

MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADVCHAYQILRKGIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVLKRKHEAKSYKSMVIYVEACEAGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPHTGYDTCLGDLYSVAWMEDSIGNNFLNTYFGNTSDDNNNESSVKSTHTISSLSNGVSQRDASLLHYWYKFQNAPYGSREKMEASEQLEDEILSRRHADNSINQIGELLFGEAKSSRVLNSIRGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTCVGNT
Homology
BLAST of Csor.00g215520 vs. ExPASy Swiss-Prot
Match: P49044 (Vacuolar-processing enzyme OS=Vicia sativa OX=3908 PE=1 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 3.0e-131
Identity = 233/427 (54.57%), Postives = 294/427 (68.85%), Query Frame = 0

Query: 35  DQPGKRWALLVAGSNGYDNYRHQADVCHAYQILRK-GIPDENIIVFMYDDIALHPDNPRP 94
           D  G RWA+L+AGSNGY NYRHQ+DVCHAYQ+LRK G  +ENIIVFMYDDIA + +NPRP
Sbjct: 51  DFEGTRWAILLAGSNGYWNYRHQSDVCHAYQLLRKGGSKEENIIVFMYDDIASNEENPRP 110

Query: 95  GIIINKPGGADVYQGVPKDYTGKDVNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIY 154
           G+IINKP G DVY GVPKDYTG +V++ NFYAA+LGNKS +TGGSGKV+DSGPNDHIF+Y
Sbjct: 111 GVIINKPDGDDVYAGVPKDYTGAEVHADNFYAALLGNKSALTGGSGKVVDSGPNDHIFVY 170

Query: 155 YTDHGAAGMLGMPMGDYIYSNDLMDVLKRKHEAKSYKSMVIYVEACEAGSMFEGLLPENM 214
           YTDHG  G+LGMP+G Y+Y++DL +VLK+KH + +YKS+V Y+EACE+GS+FEGLLP+++
Sbjct: 171 YTDHGGPGVLGMPVGPYLYASDLNEVLKKKHASGTYKSLVFYLEACESGSIFEGLLPDDL 230

Query: 215 NIYATTASNATEDSWAAYCPGQSPTPHTGYDTCLGDLYSVAWMEDS-------------- 274
           NIYATTASNA E SW  YCPG  P P   Y TCLGDLYS+AWMEDS              
Sbjct: 231 NIYATTASNAEESSWGYYCPGDKPPPPPEYSTCLGDLYSIAWMEDSEVHNLQTESLQQQY 290

Query: 275 -----------------------IGNNFLNTYFGNTSDDNNNESSVKSTHTISSL---SN 334
                                  +  N L  Y G T+  N+N S V  T     L   S 
Sbjct: 291 KLVKNRTISEPYGSHVMEYGDIGLSKNDLYQYLG-TNPANDNNSFVDETENSLKLRTPSA 350

Query: 335 GVSQRDASLLHYWYKFQNAPYGSREKMEASEQLEDEILSRRHADNSINQIGELLFGEAKS 394
            V+QRDA L+H+W KF+ AP GS +K EA +Q+ + +  R+H DNS+  IG+LLFG  K 
Sbjct: 351 AVNQRDADLIHFWEKFRKAPEGSSQKNEAEKQVLEAMSHRKHIDNSVKLIGQLLFGIEKG 410

Query: 395 SRVLNSIRGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITMDQMD 421
           + +L+ +R  G   VD+W+C K  VK +E HC  +S+YGMK+ ++ ANICNAGI  + M 
Sbjct: 411 TELLDVVRPAGSPLVDNWDCLKTMVKTFETHCGSLSQYGMKHMRSFANICNAGIPNEPMA 470

BLAST of Csor.00g215520 vs. ExPASy Swiss-Prot
Match: Q39119 (Vacuolar-processing enzyme gamma-isozyme OS=Arabidopsis thaliana OX=3702 GN=At4g32940 PE=1 SV=2)

HSP 1 Score: 465.3 bits (1196), Expect = 7.3e-130
Identity = 229/424 (54.01%), Postives = 291/424 (68.63%), Query Frame = 0

Query: 38  GKRWALLVAGSNGYDNYRHQADVCHAYQILRK-GIPDENIIVFMYDDIALHPDNPRPGII 97
           G RWA+LVAGS+GY NYRHQAD+CHAYQ+LRK G+ +ENI+VFMYDDIA + +NPRPG I
Sbjct: 57  GTRWAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVFMYDDIANNYENPRPGTI 116

Query: 98  INKPGGADVYQGVPKDYTGKDVNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTD 157
           IN P G DVYQGVPKDYTG DVN  N +A ILG+K+ V GGSGKV+DSGPNDHIFI+Y+D
Sbjct: 117 INSPHGKDVYQGVPKDYTGDDVNVDNLFAVILGDKTAVKGGSGKVVDSGPNDHIFIFYSD 176

Query: 158 HGAAGMLGMPMGDYIYSNDLMDVLKRKHEAKSYKSMVIYVEACEAGSMFEGLLPENMNIY 217
           HG  G+LGMP   Y+Y+NDL DVLK+KH   +YKS+V Y+EACE+GS+FEGLLPE +NIY
Sbjct: 177 HGGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLVFYLEACESGSIFEGLLPEGLNIY 236

Query: 218 ATTASNATEDSWAAYCPGQSPTPHTGYDTCLGDLYSVAWMEDS----------------- 277
           ATTASNA E SW  YCPG+ P+P   Y+TCLGDLYSVAWMEDS                 
Sbjct: 237 ATTASNAEESSWGTYCPGEEPSPPPEYETCLGDLYSVAWMEDSGMHNLQTETLHQQYELV 296

Query: 278 ----------------------IGNNFLNTYFG-NTSDDNNNESSVKSTHTISSLSNGVS 337
                                 I  + L+ Y G N ++DN   +   S    S ++N   
Sbjct: 297 KRRTAPVGYSYGSHVMQYGDVGISKDNLDLYMGTNPANDNFTFADANSLKPPSRVTN--- 356

Query: 338 QRDASLLHYWYKFQNAPYGSREKMEASEQLEDEILSRRHADNSINQIGELLFGEAKSSRV 397
           QRDA L+H+W K++ AP GS  K EA +Q+ + +  R H DNS+  +G++LFG ++   V
Sbjct: 357 QRDADLVHFWEKYRKAPEGSARKTEAQKQVLEAMSHRLHIDNSVILVGKILFGISRGPEV 416

Query: 398 LNSIRGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITMDQMDQAC 421
           LN +R  GQ  VDDWNC K  V+ +E+HC  +S+YG+K+ ++ ANICNAGI M+QM++A 
Sbjct: 417 LNKVRSAGQPLVDDWNCLKNQVRAFERHCGSLSQYGIKHMRSFANICNAGIQMEQMEEAA 476

BLAST of Csor.00g215520 vs. ExPASy Swiss-Prot
Match: P49043 (Vacuolar-processing enzyme OS=Citrus sinensis OX=2711 PE=2 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 2.4e-128
Identity = 227/425 (53.41%), Postives = 290/425 (68.24%), Query Frame = 0

Query: 35  DQPGKRWALLVAGSNGYDNYRHQADVCHAYQILRK-GIPDENIIVFMYDDIALHPDNPRP 94
           D  G RWA+L+AGSNG+ NYRHQAD+CHAYQ+LRK G+ DENIIVFMYDDIA + +NPRP
Sbjct: 55  DSVGTRWAVLLAGSNGFWNYRHQADICHAYQLLRKGGLKDENIIVFMYDDIAFNEENPRP 114

Query: 95  GIIINKPGGADVYQGVPKDYTGKDVNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIY 154
           G+IIN P G DVY+GVPKDYTG+DV    F+A +LGNK+ +TGGSGKV+DSGPNDHIFI+
Sbjct: 115 GVIINHPHGDDVYKGVPKDYTGEDVTVEKFFAVVLGNKTALTGGSGKVVDSGPNDHIFIF 174

Query: 155 YTDHGAAGMLGMPMGDYIYSNDLMDVLKRKHEAKSYKSMVIYVEACEAGSMFEGLLPENM 214
           Y+DHG  G+LGMP   YIY+++L+DVLK+KH + +YKS+V Y+EACE+GS+FEGLL E +
Sbjct: 175 YSDHGGPGVLGMPTSRYIYADELIDVLKKKHASGNYKSLVFYLEACESGSIFEGLLLEGL 234

Query: 215 NIYATTASNATEDSWAAYCPGQSPTPHTGYDTCLGDLYSVAWMEDS-------------- 274
           NIYATTASNA E SW  YCPG+ P P   Y TCLGDLYS+AWMEDS              
Sbjct: 235 NIYATTASNAEESSWGTYCPGEIPGPPPEYSTCLGDLYSIAWMEDSDIHNLRTETLHQQY 294

Query: 275 ------------------------IGNNFLNTYFGNTSDDNNNESSVKSTHTISSLSNGV 334
                                   +  N L TY G T+  N+N + V   +++   S  V
Sbjct: 295 ELVKTRTASYNSYGSHVMQYGDIGLSKNNLFTYLG-TNPANDNYTFV-DENSLRPASKAV 354

Query: 335 SQRDASLLHYWYKFQNAPYGSREKMEASEQLEDEILSRRHADNSINQIGELLFGEAKSSR 394
           +QRDA LLH+W K++ AP G+  K EA +Q  + +  R H D+SI  IG+LLFG  K   
Sbjct: 355 NQRDADLLHFWDKYRKAPEGTPRKAEAQKQFFEAMSHRMHVDHSIKLIGKLLFGIEKGPE 414

Query: 395 VLNSIRGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITMDQMDQA 421
           +LN++R  GQ  VDDW C K  V+ +E HC  +S+YGMK+ ++LANICN GI  ++M +A
Sbjct: 415 ILNTVRPAGQPLVDDWGCLKSLVRTFESHCGALSQYGMKHMRSLANICNTGIGKEKMAEA 474

BLAST of Csor.00g215520 vs. ExPASy Swiss-Prot
Match: O24325 (Vacuolar-processing enzyme OS=Phaseolus vulgaris OX=3885 PE=1 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 2.6e-127
Identity = 229/464 (49.35%), Postives = 311/464 (67.03%), Query Frame = 0

Query: 6   TTPTTLLLIALLFFNSIIAKKQWV----------SHANTDQPGKRWALLVAGSNGYDNYR 65
           T  T+LL + LLF  ++++  + +          S    +  G RWA+L AGS+GY NYR
Sbjct: 5   TATTSLLALLLLFLVALVSAGRDLVGDFLRLPSDSGNGDNVHGTRWAILFAGSSGYWNYR 64

Query: 66  HQADVCHAYQILRK-GIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYT 125
           HQAD+CHAYQ+LRK G+ DENIIVFMYDDIA + +NPR G+IIN P G +VY+GVPKDYT
Sbjct: 65  HQADICHAYQLLRKGGLKDENIIVFMYDDIAFNSENPRRGVIINSPNGDEVYKGVPKDYT 124

Query: 126 GKDVNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSN 185
           G+DV + NFYAA+LG+KS +TGGSGKV++SGPNDHIFI+Y+DHG  G+LG P G YIY++
Sbjct: 125 GEDVTAHNFYAALLGDKSKLTGGSGKVVNSGPNDHIFIFYSDHGGPGVLGSPAGPYIYAS 184

Query: 186 DLMDVLKRKHEAKSYKSMVIYVEACEAGSMFEGLLPENMNIYATTASNATEDSWAAYCPG 245
           DL +VLK+KH + +YK++V Y+EACE+GS+FEGLLPE++N+YATTASNA E SW  YCPG
Sbjct: 185 DLNEVLKKKHASGTYKNLVFYLEACESGSIFEGLLPEDINVYATTASNADESSWGTYCPG 244

Query: 246 QSPTPHTGYDTCLGDLYSVAWMEDSIGNNFL------------------NTYFGN----- 305
           + P+P   Y TCLGDLYSVAWMEDS  +N                      Y+G+     
Sbjct: 245 EDPSPPPEYSTCLGDLYSVAWMEDSDRHNLRTETLHQQYKLVKERTISGGLYYGSHVMQY 304

Query: 306 --------------TSDDNNNESSVKSTHTISSLSNGVSQRDASLLHYWYKFQNAPYGSR 365
                          +D  N   +    +++ S S  V+QRDA L+H+W KF+ AP GS 
Sbjct: 305 GDVGLSKDILFHYLGTDPANENLTFVDENSLWSSSKAVNQRDADLVHFWDKFRKAPEGSP 364

Query: 366 EKMEASEQLEDEILSRRHADNSINQIGELLFGEAKSSRVLNSIRGHGQSFVDDWNCFKKY 422
           +K EA +Q+ + +  R H D+S+  +G+LLFG  K+  +LN++R  G + VDDW+C K  
Sbjct: 365 KKNEARKQVLEVMSHRMHIDDSVELVGKLLFGIEKAPELLNAVRPAGSALVDDWDCLKTM 424

BLAST of Csor.00g215520 vs. ExPASy Swiss-Prot
Match: P49047 (Vacuolar-processing enzyme alpha-isozyme OS=Arabidopsis thaliana OX=3702 GN=At2g25940 PE=2 SV=2)

HSP 1 Score: 452.2 bits (1162), Expect = 6.4e-126
Identity = 223/430 (51.86%), Postives = 291/430 (67.67%), Query Frame = 0

Query: 35  DQPGKRWALLVAGSNGYDNYRHQADVCHAYQILRK-GIPDENIIVFMYDDIALHPDNPRP 94
           D    +WA+LVAGS+GY NYRHQADVCHAYQ+L+K G+ +ENI+VFMYDDIA + +NPRP
Sbjct: 39  DDDSTKWAVLVAGSSGYWNYRHQADVCHAYQLLKKGGVKEENIVVFMYDDIAKNEENPRP 98

Query: 95  GIIINKPGGADVYQGVPKDYTGKDVNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIY 154
           G+IIN P G DVY GVPKDYTG +VN  N  A ILGNK+ + GGSGKV+DSGPNDHIFIY
Sbjct: 99  GVIINSPNGEDVYNGVPKDYTGDEVNVDNLLAVILGNKTALKGGSGKVVDSGPNDHIFIY 158

Query: 155 YTDHGAAGMLGMPMGDYIYSNDLMDVLKRKHEAKSYKSMVIYVEACEAGSMFEGLLPENM 214
           Y+DHG  G+LGMP    +Y+NDL DVLK+K+ + +YKS+V Y+EACE+GS+FEGLLPE +
Sbjct: 159 YSDHGGPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFYLEACESGSIFEGLLPEGL 218

Query: 215 NIYATTASNATEDSWAAYCPGQSPTPHTGYDTCLGDLYSVAWMEDSIGNNF--------- 274
           NIYATTASNA E SW  YCPG+ P+P + Y+TCLGDLYSVAW+EDS  +N          
Sbjct: 219 NIYATTASNAEESSWGTYCPGEDPSPPSEYETCLGDLYSVAWIEDSEKHNLQTETLHEQY 278

Query: 275 ------------------------------LNTYFGNTSDDNN----NESSVKSTHTISS 334
                                         L  + G    D N    NE+S++    ++ 
Sbjct: 279 ELVKKRTAGSGKSYGSHVMEFGDIGLSKEKLVLFMGTNPADENFTFVNENSIRPPSRVT- 338

Query: 335 LSNGVSQRDASLLHYWYKFQNAPYGSREKMEASEQLEDEILSRRHADNSINQIGELLFGE 394
                +QRDA L+H+W+K+Q AP GS  K+EA +Q+ + +  R H DNSI  IG LLFG 
Sbjct: 339 -----NQRDADLVHFWHKYQKAPEGSARKVEAQKQVLEAMSHRLHVDNSILLIGILLFG- 398

Query: 395 AKSSRVLNSIRGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITMD 421
            +   VLN +R  G+  VDDW+C K  V+ +E+HC  +S+YG+K+ +++AN+CNAGI M 
Sbjct: 399 LEGHAVLNKVRPSGEPLVDDWDCLKSLVRAFERHCGSLSQYGIKHMRSIANMCNAGIQMR 458

BLAST of Csor.00g215520 vs. NCBI nr
Match: KAG6589920.1 (hypothetical protein SDJN03_15343, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 871 bits (2250), Expect = 0.0
Identity = 424/424 (100.00%), Postives = 424/424 (100.00%), Query Frame = 0

Query: 1   MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADV 60
           MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADV
Sbjct: 1   MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADV 60

Query: 61  CHAYQILRKGIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 120
           CHAYQILRKGIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS
Sbjct: 61  CHAYQILRKGIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 120

Query: 121 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 180
           VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL
Sbjct: 121 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 180

Query: 181 KRKHEAKSYKSMVIYVEACEAGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 240
           KRKHEAKSYKSMVIYVEACEAGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH
Sbjct: 181 KRKHEAKSYKSMVIYVEACEAGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 240

Query: 241 TGYDTCLGDLYSVAWMEDSIGNNFLNTYFGNTSDDNNNESSVKSTHTISSLSNGVSQRDA 300
           TGYDTCLGDLYSVAWMEDSIGNNFLNTYFGNTSDDNNNESSVKSTHTISSLSNGVSQRDA
Sbjct: 241 TGYDTCLGDLYSVAWMEDSIGNNFLNTYFGNTSDDNNNESSVKSTHTISSLSNGVSQRDA 300

Query: 301 SLLHYWYKFQNAPYGSREKMEASEQLEDEILSRRHADNSINQIGELLFGEAKSSRVLNSI 360
           SLLHYWYKFQNAPYGSREKMEASEQLEDEILSRRHADNSINQIGELLFGEAKSSRVLNSI
Sbjct: 301 SLLHYWYKFQNAPYGSREKMEASEQLEDEILSRRHADNSINQIGELLFGEAKSSRVLNSI 360

Query: 361 RGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTC 420
           RGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTC
Sbjct: 361 RGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTC 420

Query: 421 VGNT 424
           VGNT
Sbjct: 421 VGNT 424

BLAST of Csor.00g215520 vs. NCBI nr
Match: XP_022961313.1 (vacuolar-processing enzyme gamma-isozyme-like [Cucurbita moschata])

HSP 1 Score: 841 bits (2173), Expect = 1.13e-306
Identity = 417/463 (90.06%), Postives = 422/463 (91.14%), Query Frame = 0

Query: 1   MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADV 60
           MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADV
Sbjct: 19  MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADV 78

Query: 61  CHAYQILRKGIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 120
           CHAYQILRKGIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS
Sbjct: 79  CHAYQILRKGIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 138

Query: 121 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 180
           VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL
Sbjct: 139 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 198

Query: 181 KRKHEAKSYKSMVIYVEACEAGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 240
           KRKHEAKSYKSMVIYVEACEAGS+FEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH
Sbjct: 199 KRKHEAKSYKSMVIYVEACEAGSLFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 258

Query: 241 TGYDTCLGDLYSVAWMEDS---------------------------------------IG 300
           TGYDTCLGDLYSVAWMEDS                                       IG
Sbjct: 259 TGYDTCLGDLYSVAWMEDSDKHDSSKETLNQQYHTVRRRTSVDKVGYGSHVMLYGNESIG 318

Query: 301 NNFLNTYFGNTSDDNNNESSVKSTHTISSLSNGVSQRDASLLHYWYKFQNAPYGSREKME 360
           NNFLNTYFGNTSDDNNNESSVKSTH ISSLSNGVSQRDASLLHYWYKFQNAPYGSREKME
Sbjct: 319 NNFLNTYFGNTSDDNNNESSVKSTHIISSLSNGVSQRDASLLHYWYKFQNAPYGSREKME 378

Query: 361 ASEQLEDEILSRRHADNSINQIGELLFGEAKSSRVLNSIRGHGQSFVDDWNCFKKYVKIY 420
           ASE+LEDEILSRRHADNS+NQIGELLFGEAKSSRVLN IRGHGQSFVDDWNCFKKYVKIY
Sbjct: 379 ASERLEDEILSRRHADNSVNQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKYVKIY 438

Query: 421 EKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTCVGNT 424
           EKHCKRMSRYGMKYTQALANICNAGIT+DQMD+ACLQTCVGNT
Sbjct: 439 EKHCKRMSRYGMKYTQALANICNAGITIDQMDKACLQTCVGNT 481

BLAST of Csor.00g215520 vs. NCBI nr
Match: XP_023516171.1 (vacuolar-processing enzyme gamma-isozyme-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 833 bits (2152), Expect = 9.08e-304
Identity = 414/463 (89.42%), Postives = 419/463 (90.50%), Query Frame = 0

Query: 1   MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADV 60
           MTTFATTPTTLLLIALLFFNSIIAKKQWVSHAN DQPGKRWALLVAGSNGYDNYRHQADV
Sbjct: 1   MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANADQPGKRWALLVAGSNGYDNYRHQADV 60

Query: 61  CHAYQILRKGIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 120
           CHAYQILRKGIPDENIIVFMYDDIA  PDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS
Sbjct: 61  CHAYQILRKGIPDENIIVFMYDDIAFDPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 120

Query: 121 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 180
           VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL
Sbjct: 121 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 180

Query: 181 KRKHEAKSYKSMVIYVEACEAGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 240
           KRKHEAKSYKSMVIYVEACE+GSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH
Sbjct: 181 KRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 240

Query: 241 TGYDTCLGDLYSVAWMEDS---------------------------------------IG 300
           TGYDTCLGDLYSVAWMEDS                                       IG
Sbjct: 241 TGYDTCLGDLYSVAWMEDSDKHDSSKETLNQQYHTVRRRTSVDKVGYGSHVMLYGNESIG 300

Query: 301 NNFLNTYFGNTSDDNNNESSVKSTHTISSLSNGVSQRDASLLHYWYKFQNAPYGSREKME 360
           NNFLNTYFGN+SDDNNNESSVKSTH ISSL+NGVSQRDASLLHYWYKFQNAPYGSREKME
Sbjct: 301 NNFLNTYFGNSSDDNNNESSVKSTHIISSLANGVSQRDASLLHYWYKFQNAPYGSREKME 360

Query: 361 ASEQLEDEILSRRHADNSINQIGELLFGEAKSSRVLNSIRGHGQSFVDDWNCFKKYVKIY 420
           ASE+LEDEILSRRHADNSINQIGELLFGEAKSSRVLN IRGHGQSFVDDWNCFKKYVKIY
Sbjct: 361 ASERLEDEILSRRHADNSINQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKYVKIY 420

Query: 421 EKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTCVGNT 424
           EKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTCVG+T
Sbjct: 421 EKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTCVGST 463

BLAST of Csor.00g215520 vs. NCBI nr
Match: XP_022987711.1 (vacuolar-processing enzyme gamma-isozyme-like [Cucurbita maxima])

HSP 1 Score: 825 bits (2132), Expect = 1.01e-300
Identity = 410/463 (88.55%), Postives = 413/463 (89.20%), Query Frame = 0

Query: 1   MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADV 60
           MTTFATTPTTLLLI LLFFNSIIAKK+WVSHANTDQPGKRWALLVAGSNGYDNYRHQADV
Sbjct: 1   MTTFATTPTTLLLIPLLFFNSIIAKKEWVSHANTDQPGKRWALLVAGSNGYDNYRHQADV 60

Query: 61  CHAYQILRKGIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 120
           CHAYQILRKGIPDENIIVFMYDDIA HPDNPRPGIIINKPGGADVY GVPKDYTGKDVNS
Sbjct: 61  CHAYQILRKGIPDENIIVFMYDDIAFHPDNPRPGIIINKPGGADVYHGVPKDYTGKDVNS 120

Query: 121 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 180
           VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL
Sbjct: 121 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 180

Query: 181 KRKHEAKSYKSMVIYVEACEAGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 240
           KRKHEAKSYKSMVIYVEACE+GSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH
Sbjct: 181 KRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 240

Query: 241 TGYDTCLGDLYSVAWMEDS---------------------------------------IG 300
           TGYDTCLGDLYSVAWMEDS                                        G
Sbjct: 241 TGYDTCLGDLYSVAWMEDSDKHDSSKETLNQQYHTVRRRTSVDKVGYGSHVMLYGNESFG 300

Query: 301 NNFLNTYFGNTSDDNNNESSVKSTHTISSLSNGVSQRDASLLHYWYKFQNAPYGSREKME 360
           NNFLNTYFGNTSDDNNNESSVKSTH ISSLSN VSQRDASLLHYWYKFQNAPYGSREKME
Sbjct: 301 NNFLNTYFGNTSDDNNNESSVKSTHIISSLSNAVSQRDASLLHYWYKFQNAPYGSREKME 360

Query: 361 ASEQLEDEILSRRHADNSINQIGELLFGEAKSSRVLNSIRGHGQSFVDDWNCFKKYVKIY 420
           ASEQLEDEILSRRHADNSINQIG+LLFGEAKSSRVL   RGHGQSFVDDWNCFK YVKIY
Sbjct: 361 ASEQLEDEILSRRHADNSINQIGDLLFGEAKSSRVLYRSRGHGQSFVDDWNCFKNYVKIY 420

Query: 421 EKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTCVGNT 424
           EKHCKRMSRYGMKYTQALAN CNAGITMDQMDQACLQTCVGNT
Sbjct: 421 EKHCKRMSRYGMKYTQALANTCNAGITMDQMDQACLQTCVGNT 463

BLAST of Csor.00g215520 vs. NCBI nr
Match: KAG7023589.1 (hypothetical protein SDJN02_14615, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 756 bits (1952), Expect = 2.58e-273
Identity = 378/417 (90.65%), Postives = 378/417 (90.65%), Query Frame = 0

Query: 1   MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADV 60
           MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADV
Sbjct: 19  MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADV 78

Query: 61  CHAYQILRKGIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 120
           CHAYQILRKGIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS
Sbjct: 79  CHAYQILRKGIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 138

Query: 121 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 180
           VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL
Sbjct: 139 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 198

Query: 181 KRKHEAKSYKSMVIYVEACEAGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 240
           KRKHEAKSYKSMVIYVEACEAGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH
Sbjct: 199 KRKHEAKSYKSMVIYVEACEAGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 258

Query: 241 TGYDTCLGDLYSVAWMEDS---------------------------------------IG 300
           TGYDTCLGDLYSVAWMEDS                                       IG
Sbjct: 259 TGYDTCLGDLYSVAWMEDSDKHDSSKETLNQQYHTVRRRTSVDKVGYGSHVMLYGNESIG 318

Query: 301 NNFLNTYFGNTSDDNNNESSVKSTHTISSLSNGVSQRDASLLHYWYKFQNAPYGSREKME 360
           NNFLNTYFGNTSDDNNNESSVKSTHTISSLSNGVSQRDASLLHYWYKFQNAPYGSREKME
Sbjct: 319 NNFLNTYFGNTSDDNNNESSVKSTHTISSLSNGVSQRDASLLHYWYKFQNAPYGSREKME 378

Query: 361 ASEQLEDEILSRRHADNSINQIGELLFGEAKSSRVLNSIRGHGQSFVDDWNCFKKYV 378
           ASEQLEDEILSRRHADNSINQIGELLFGEAKSSRVLNSIRGHGQSFVDDWNCFKKYV
Sbjct: 379 ASEQLEDEILSRRHADNSINQIGELLFGEAKSSRVLNSIRGHGQSFVDDWNCFKKYV 435

BLAST of Csor.00g215520 vs. ExPASy TrEMBL
Match: A0A6J1HBF5 (vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita moschata OX=3662 GN=LOC111461846 PE=3 SV=1)

HSP 1 Score: 841 bits (2173), Expect = 5.47e-307
Identity = 417/463 (90.06%), Postives = 422/463 (91.14%), Query Frame = 0

Query: 1   MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADV 60
           MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADV
Sbjct: 19  MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADV 78

Query: 61  CHAYQILRKGIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 120
           CHAYQILRKGIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS
Sbjct: 79  CHAYQILRKGIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 138

Query: 121 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 180
           VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL
Sbjct: 139 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 198

Query: 181 KRKHEAKSYKSMVIYVEACEAGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 240
           KRKHEAKSYKSMVIYVEACEAGS+FEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH
Sbjct: 199 KRKHEAKSYKSMVIYVEACEAGSLFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 258

Query: 241 TGYDTCLGDLYSVAWMEDS---------------------------------------IG 300
           TGYDTCLGDLYSVAWMEDS                                       IG
Sbjct: 259 TGYDTCLGDLYSVAWMEDSDKHDSSKETLNQQYHTVRRRTSVDKVGYGSHVMLYGNESIG 318

Query: 301 NNFLNTYFGNTSDDNNNESSVKSTHTISSLSNGVSQRDASLLHYWYKFQNAPYGSREKME 360
           NNFLNTYFGNTSDDNNNESSVKSTH ISSLSNGVSQRDASLLHYWYKFQNAPYGSREKME
Sbjct: 319 NNFLNTYFGNTSDDNNNESSVKSTHIISSLSNGVSQRDASLLHYWYKFQNAPYGSREKME 378

Query: 361 ASEQLEDEILSRRHADNSINQIGELLFGEAKSSRVLNSIRGHGQSFVDDWNCFKKYVKIY 420
           ASE+LEDEILSRRHADNS+NQIGELLFGEAKSSRVLN IRGHGQSFVDDWNCFKKYVKIY
Sbjct: 379 ASERLEDEILSRRHADNSVNQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKYVKIY 438

Query: 421 EKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTCVGNT 424
           EKHCKRMSRYGMKYTQALANICNAGIT+DQMD+ACLQTCVGNT
Sbjct: 439 EKHCKRMSRYGMKYTQALANICNAGITIDQMDKACLQTCVGNT 481

BLAST of Csor.00g215520 vs. ExPASy TrEMBL
Match: A0A6J1JF37 (vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita maxima OX=3661 GN=LOC111485183 PE=3 SV=1)

HSP 1 Score: 825 bits (2132), Expect = 4.91e-301
Identity = 410/463 (88.55%), Postives = 413/463 (89.20%), Query Frame = 0

Query: 1   MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADV 60
           MTTFATTPTTLLLI LLFFNSIIAKK+WVSHANTDQPGKRWALLVAGSNGYDNYRHQADV
Sbjct: 1   MTTFATTPTTLLLIPLLFFNSIIAKKEWVSHANTDQPGKRWALLVAGSNGYDNYRHQADV 60

Query: 61  CHAYQILRKGIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 120
           CHAYQILRKGIPDENIIVFMYDDIA HPDNPRPGIIINKPGGADVY GVPKDYTGKDVNS
Sbjct: 61  CHAYQILRKGIPDENIIVFMYDDIAFHPDNPRPGIIINKPGGADVYHGVPKDYTGKDVNS 120

Query: 121 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 180
           VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL
Sbjct: 121 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 180

Query: 181 KRKHEAKSYKSMVIYVEACEAGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 240
           KRKHEAKSYKSMVIYVEACE+GSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH
Sbjct: 181 KRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 240

Query: 241 TGYDTCLGDLYSVAWMEDS---------------------------------------IG 300
           TGYDTCLGDLYSVAWMEDS                                        G
Sbjct: 241 TGYDTCLGDLYSVAWMEDSDKHDSSKETLNQQYHTVRRRTSVDKVGYGSHVMLYGNESFG 300

Query: 301 NNFLNTYFGNTSDDNNNESSVKSTHTISSLSNGVSQRDASLLHYWYKFQNAPYGSREKME 360
           NNFLNTYFGNTSDDNNNESSVKSTH ISSLSN VSQRDASLLHYWYKFQNAPYGSREKME
Sbjct: 301 NNFLNTYFGNTSDDNNNESSVKSTHIISSLSNAVSQRDASLLHYWYKFQNAPYGSREKME 360

Query: 361 ASEQLEDEILSRRHADNSINQIGELLFGEAKSSRVLNSIRGHGQSFVDDWNCFKKYVKIY 420
           ASEQLEDEILSRRHADNSINQIG+LLFGEAKSSRVL   RGHGQSFVDDWNCFK YVKIY
Sbjct: 361 ASEQLEDEILSRRHADNSINQIGDLLFGEAKSSRVLYRSRGHGQSFVDDWNCFKNYVKIY 420

Query: 421 EKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTCVGNT 424
           EKHCKRMSRYGMKYTQALAN CNAGITMDQMDQACLQTCVGNT
Sbjct: 421 EKHCKRMSRYGMKYTQALANTCNAGITMDQMDQACLQTCVGNT 463

BLAST of Csor.00g215520 vs. ExPASy TrEMBL
Match: A0A6J1D475 (vacuolar-processing enzyme gamma-isozyme-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016825 PE=3 SV=1)

HSP 1 Score: 637 bits (1644), Expect = 1.28e-226
Identity = 317/468 (67.74%), Postives = 370/468 (79.06%), Query Frame = 0

Query: 2   TTFATTPTTLLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADVC 61
           T +AT+   +LLIALL F+ IIAK++ V  A+TDQPGKRWA+LVAGS+GY+NYRHQADVC
Sbjct: 3   TCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQADVC 62

Query: 62  HAYQILRKG-IPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 121
           HAYQILRKG +PDENIIVFMYDDIA +P NPRPG++INKP G DVYQGVPKDYTG+ VNS
Sbjct: 63  HAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHVNS 122

Query: 122 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 181
           +NFYA ILGN+S +TGGSGKV+DS  +DHIFIYYTDHG+AG+LGMP GDY+Y+ DLM+VL
Sbjct: 123 INFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLMEVL 182

Query: 182 KRKHEAKSYKSMVIYVEACEAGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 241
           K+KHEAKSYKSMVIYVEACE+GSM EGLLPEN+ IYATTASNATE+SWA YCPGQ P+P 
Sbjct: 183 KQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPSPP 242

Query: 242 TGYDTCLGDLYSVAWMEDS---------------------------------------IG 301
           T YDTCLGDLYS+AWMEDS                                       IG
Sbjct: 243 TDYDTCLGDLYSIAWMEDSDKHDLSKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIG 302

Query: 302 NNFLNTYFGNTSDDNNNESSVKSTHTISS-----LSNGVSQRDASLLHYWYKFQNAPYGS 361
           NN L+TY G   D+ N  SSV+ST TI+       SN VSQRDASL+HYW+KFQ AP+GS
Sbjct: 303 NNSLDTYIGANPDNYNYTSSVQSTDTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGS 362

Query: 362 REKMEASEQLEDEILSRRHADNSINQIGELLFGEAKSSRVLNSIRGHGQSFVDDWNCFKK 421
           REK EA +QLEDEIL+RRH D+SI  I +LLFG+AKSS VLN++R  GQS VDDW CFKK
Sbjct: 363 REKTEARKQLEDEILNRRHVDSSIYHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKK 422

Query: 422 YVKIYEKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTCVGNT 424
           +VK YEKHC+R+SRYGMKYT+ALANICNAGIT++QMDQACL+TC+  T
Sbjct: 423 FVKTYEKHCRRLSRYGMKYTRALANICNAGITINQMDQACLETCLVKT 470

BLAST of Csor.00g215520 vs. ExPASy TrEMBL
Match: A0A6J1D2Z4 (vacuolar-processing enzyme-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016825 PE=3 SV=1)

HSP 1 Score: 624 bits (1610), Expect = 6.46e-221
Identity = 317/502 (63.15%), Postives = 370/502 (73.71%), Query Frame = 0

Query: 2   TTFATTPTTLLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADVC 61
           T +AT+   +LLIALL F+ IIAK++ V  A+TDQPGKRWA+LVAGS+GY+NYRHQADVC
Sbjct: 3   TCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQADVC 62

Query: 62  HAYQILRKG-IPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 121
           HAYQILRKG +PDENIIVFMYDDIA +P NPRPG++INKP G DVYQGVPKDYTG+ VNS
Sbjct: 63  HAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHVNS 122

Query: 122 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 181
           +NFYA ILGN+S +TGGSGKV+DS  +DHIFIYYTDHG+AG+LGMP GDY+Y+ DLM+VL
Sbjct: 123 INFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLMEVL 182

Query: 182 KRKHEAKSYKSMVIYVEACEAGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 241
           K+KHEAKSYKSMVIYVEACE+GSM EGLLPEN+ IYATTASNATE+SWA YCPGQ P+P 
Sbjct: 183 KQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPSPP 242

Query: 242 TGYDTCLGDLYSVAWMED------------------------------------------ 301
           T YDTCLGDLYS+AWMED                                          
Sbjct: 243 TDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSDKHDLSK 302

Query: 302 -------------------------------SIGNNFLNTYFGNTSDDNNNESSVKSTHT 361
                                          SIGNN L+TY G   D+ N  SSV+ST T
Sbjct: 303 ETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQSTDT 362

Query: 362 ISS-----LSNGVSQRDASLLHYWYKFQNAPYGSREKMEASEQLEDEILSRRHADNSINQ 421
           I+       SN VSQRDASL+HYW+KFQ AP+GSREK EA +QLEDEIL+RRH D+SI  
Sbjct: 363 IAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDSSIYH 422

Query: 422 IGELLFGEAKSSRVLNSIRGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANI 424
           I +LLFG+AKSS VLN++R  GQS VDDW CFKK+VK YEKHC+R+SRYGMKYT+ALANI
Sbjct: 423 IAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRALANI 482

BLAST of Csor.00g215520 vs. ExPASy TrEMBL
Match: A0A5A7TXA7 (Vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold114G00680 PE=3 SV=1)

HSP 1 Score: 620 bits (1599), Expect = 5.81e-220
Identity = 318/469 (67.80%), Postives = 357/469 (76.12%), Query Frame = 0

Query: 1   MTTFATTPTTLLLIALLF--FNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQA 60
           MTT    PT LL+I + F  FN+IIAK +W+S A TDQ GK WALL+AGSNGYDNYRHQ 
Sbjct: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQG 60

Query: 61  DVCHAYQILRKGIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDV 120
                      GIPDENIIVFMYDDIA HP NPRPGIIINKP G DVY GVPKDYTGK V
Sbjct: 61  -----------GIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGNDVYHGVPKDYTGKHV 120

Query: 121 NSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMD 180
           N+VNFYAAILGNKS +TGGSGKVI SGP DHIFIYY+DHGAAGMLGMP GDYI+ NDL+D
Sbjct: 121 NAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLID 180

Query: 181 VLKRKHEAKSYKSMVIYVEACEAGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPT 240
           VLKRKH+AK+YKSMVIYVEACE+GSMFEG+LPEN+ IYATTASNATEDSWA YCP QS  
Sbjct: 181 VLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSHR 240

Query: 241 PHTGYDTCLGDLYSVAWMEDS--------------------------------------- 300
               Y+TCLGDL+SVAWMEDS                                       
Sbjct: 241 SPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNKR 300

Query: 301 IGNNFLNTYFGNTSDDNNNESSVKSTHTISSLS----NGVSQRDASLLHYWYKFQNAPYG 360
           IGN+FL+T+ GN+ D +N + SV+S  TISSLS    N VSQRDASL+HY YKFQ AP+G
Sbjct: 301 IGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPFG 360

Query: 361 SREKMEASEQLEDEILSRRHADNSINQIGELLFGEAKSSRVLNSIRGHGQSFVDDWNCFK 420
           SREKMEA EQLEDEI  RRHADNSIN IG+LLFG+A+SS +L+++R  GQS VDDW+CFK
Sbjct: 361 SREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCFK 420

Query: 421 KYVKIYEKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTCVGNT 424
            +VKIYEKHC RMS YGMKYT+A+ANICNAGITM QMDQACLQTC+G T
Sbjct: 421 TFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCLGKT 458

BLAST of Csor.00g215520 vs. TAIR 10
Match: AT4G32940.1 (gamma vacuolar processing enzyme )

HSP 1 Score: 465.3 bits (1196), Expect = 5.2e-131
Identity = 229/424 (54.01%), Postives = 291/424 (68.63%), Query Frame = 0

Query: 38  GKRWALLVAGSNGYDNYRHQADVCHAYQILRK-GIPDENIIVFMYDDIALHPDNPRPGII 97
           G RWA+LVAGS+GY NYRHQAD+CHAYQ+LRK G+ +ENI+VFMYDDIA + +NPRPG I
Sbjct: 57  GTRWAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVFMYDDIANNYENPRPGTI 116

Query: 98  INKPGGADVYQGVPKDYTGKDVNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTD 157
           IN P G DVYQGVPKDYTG DVN  N +A ILG+K+ V GGSGKV+DSGPNDHIFI+Y+D
Sbjct: 117 INSPHGKDVYQGVPKDYTGDDVNVDNLFAVILGDKTAVKGGSGKVVDSGPNDHIFIFYSD 176

Query: 158 HGAAGMLGMPMGDYIYSNDLMDVLKRKHEAKSYKSMVIYVEACEAGSMFEGLLPENMNIY 217
           HG  G+LGMP   Y+Y+NDL DVLK+KH   +YKS+V Y+EACE+GS+FEGLLPE +NIY
Sbjct: 177 HGGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLVFYLEACESGSIFEGLLPEGLNIY 236

Query: 218 ATTASNATEDSWAAYCPGQSPTPHTGYDTCLGDLYSVAWMEDS----------------- 277
           ATTASNA E SW  YCPG+ P+P   Y+TCLGDLYSVAWMEDS                 
Sbjct: 237 ATTASNAEESSWGTYCPGEEPSPPPEYETCLGDLYSVAWMEDSGMHNLQTETLHQQYELV 296

Query: 278 ----------------------IGNNFLNTYFG-NTSDDNNNESSVKSTHTISSLSNGVS 337
                                 I  + L+ Y G N ++DN   +   S    S ++N   
Sbjct: 297 KRRTAPVGYSYGSHVMQYGDVGISKDNLDLYMGTNPANDNFTFADANSLKPPSRVTN--- 356

Query: 338 QRDASLLHYWYKFQNAPYGSREKMEASEQLEDEILSRRHADNSINQIGELLFGEAKSSRV 397
           QRDA L+H+W K++ AP GS  K EA +Q+ + +  R H DNS+  +G++LFG ++   V
Sbjct: 357 QRDADLVHFWEKYRKAPEGSARKTEAQKQVLEAMSHRLHIDNSVILVGKILFGISRGPEV 416

Query: 398 LNSIRGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITMDQMDQAC 421
           LN +R  GQ  VDDWNC K  V+ +E+HC  +S+YG+K+ ++ ANICNAGI M+QM++A 
Sbjct: 417 LNKVRSAGQPLVDDWNCLKNQVRAFERHCGSLSQYGIKHMRSFANICNAGIQMEQMEEAA 476

BLAST of Csor.00g215520 vs. TAIR 10
Match: AT2G25940.1 (alpha-vacuolar processing enzyme )

HSP 1 Score: 452.2 bits (1162), Expect = 4.6e-127
Identity = 223/430 (51.86%), Postives = 291/430 (67.67%), Query Frame = 0

Query: 35  DQPGKRWALLVAGSNGYDNYRHQADVCHAYQILRK-GIPDENIIVFMYDDIALHPDNPRP 94
           D    +WA+LVAGS+GY NYRHQADVCHAYQ+L+K G+ +ENI+VFMYDDIA + +NPRP
Sbjct: 39  DDDSTKWAVLVAGSSGYWNYRHQADVCHAYQLLKKGGVKEENIVVFMYDDIAKNEENPRP 98

Query: 95  GIIINKPGGADVYQGVPKDYTGKDVNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIY 154
           G+IIN P G DVY GVPKDYTG +VN  N  A ILGNK+ + GGSGKV+DSGPNDHIFIY
Sbjct: 99  GVIINSPNGEDVYNGVPKDYTGDEVNVDNLLAVILGNKTALKGGSGKVVDSGPNDHIFIY 158

Query: 155 YTDHGAAGMLGMPMGDYIYSNDLMDVLKRKHEAKSYKSMVIYVEACEAGSMFEGLLPENM 214
           Y+DHG  G+LGMP    +Y+NDL DVLK+K+ + +YKS+V Y+EACE+GS+FEGLLPE +
Sbjct: 159 YSDHGGPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFYLEACESGSIFEGLLPEGL 218

Query: 215 NIYATTASNATEDSWAAYCPGQSPTPHTGYDTCLGDLYSVAWMEDSIGNNF--------- 274
           NIYATTASNA E SW  YCPG+ P+P + Y+TCLGDLYSVAW+EDS  +N          
Sbjct: 219 NIYATTASNAEESSWGTYCPGEDPSPPSEYETCLGDLYSVAWIEDSEKHNLQTETLHEQY 278

Query: 275 ------------------------------LNTYFGNTSDDNN----NESSVKSTHTISS 334
                                         L  + G    D N    NE+S++    ++ 
Sbjct: 279 ELVKKRTAGSGKSYGSHVMEFGDIGLSKEKLVLFMGTNPADENFTFVNENSIRPPSRVT- 338

Query: 335 LSNGVSQRDASLLHYWYKFQNAPYGSREKMEASEQLEDEILSRRHADNSINQIGELLFGE 394
                +QRDA L+H+W+K+Q AP GS  K+EA +Q+ + +  R H DNSI  IG LLFG 
Sbjct: 339 -----NQRDADLVHFWHKYQKAPEGSARKVEAQKQVLEAMSHRLHVDNSILLIGILLFG- 398

Query: 395 AKSSRVLNSIRGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITMD 421
            +   VLN +R  G+  VDDW+C K  V+ +E+HC  +S+YG+K+ +++AN+CNAGI M 
Sbjct: 399 LEGHAVLNKVRPSGEPLVDDWDCLKSLVRAFERHCGSLSQYGIKHMRSIANMCNAGIQMR 458

BLAST of Csor.00g215520 vs. TAIR 10
Match: AT1G62710.1 (beta vacuolar processing enzyme )

HSP 1 Score: 419.9 bits (1078), Expect = 2.5e-117
Identity = 219/463 (47.30%), Postives = 290/463 (62.63%), Query Frame = 0

Query: 8   PTTLLLIALL--------FFNSIIAKKQWVSHANTDQP--GKRWALLVAGSNGYDNYRHQ 67
           P  LLL+ LL        F   I+   +  + A+ D+   G RWA+LVAGS+GY NYRHQ
Sbjct: 9   PALLLLLVLLVHAESRGRFEPKILMPTEEANPADQDEDGVGTRWAVLVAGSSGYGNYRHQ 68

Query: 68  ADVCHAYQILRK-GIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGK 127
           ADVCHAYQILRK G+ +ENI+V MYDDIA HP NPRPG +IN P G DVY GVPKDYTG 
Sbjct: 69  ADVCHAYQILRKGGLKEENIVVLMYDDIANHPLNPRPGTLINHPDGDDVYAGVPKDYTGS 128

Query: 128 DVNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDL 187
            V + NFYA +LG++  V GGSGKVI S PNDHIF+YY DHG  G+LGMP   +IY+ D 
Sbjct: 129 SVTAANFYAVLLGDQKAVKGGSGKVIASKPNDHIFVYYADHGGPGVLGMPNTPHIYAADF 188

Query: 188 MDVLKRKHEAKSYKSMVIYVEACEAGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQS 247
           ++ LK+KH + +YK MVIYVEACE+GS+FEG++P+++NIY TTASNA E S+  YCPG +
Sbjct: 189 IETLKKKHASGTYKEMVIYVEACESGSIFEGIMPKDLNIYVTTASNAQESSYGTYCPGMN 248

Query: 248 PTPHTGYDTCLGDLYSVAWMEDSIGNNF------------------LNTYFGNTSDDNNN 307
           P+P + Y TCLGDLYSVAWMEDS  +N                    NTY G +      
Sbjct: 249 PSPPSEYITCLGDLYSVAWMEDSETHNLKKETIKQQYHTVKMRTSNYNTYSGGSHVMEYG 308

Query: 308 ESSVKSTH-------------------TISSLSNGVSQRDASLLHYWYKFQNAPYGSREK 367
            +S+KS                      + S    V+QRDA LL  W+ ++ +  GSR+K
Sbjct: 309 NNSIKSEKLYLYQGFDPATVNLPLNELPVKSKIGVVNQRDADLLFLWHMYRTSEDGSRKK 368

Query: 368 MEASEQLEDEILSRRHADNSINQIGELLFGEAKSSRVLNSIRGHGQSFVDDWNCFKKYVK 423
            +  ++L +    R+H D S+  I  +LFG   +  VLN +R  G   VDDW C K  V+
Sbjct: 369 DDTLKELTETTRHRKHLDASVELIATILFG--PTMNVLNLVREPGLPLVDDWECLKSMVR 428

BLAST of Csor.00g215520 vs. TAIR 10
Match: AT3G20210.1 (delta vacuolar processing enzyme )

HSP 1 Score: 405.6 bits (1041), Expect = 4.9e-113
Identity = 218/454 (48.02%), Postives = 279/454 (61.45%), Query Frame = 0

Query: 11  LLLIALLFFNSIIAKKQW-----VSHANTDQPGKRWALLVAGSNGYDNYRHQADVCHAYQ 70
           + L ALL F++   K Q      V  ++    G RWA+LVAGSN Y NYRHQAD+CHAYQ
Sbjct: 12  VFLHALLIFSAESRKTQLLNDNDVESSDKSAKGTRWAVLVAGSNEYYNYRHQADICHAYQ 71

Query: 71  ILRK-GIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNSVNFY 130
           ILRK G+ DENIIVFMYDDIA   +NPRPG+IINKP G DVY+GVPKDYT + VN  NFY
Sbjct: 72  ILRKGGLKDENIIVFMYDDIAFSSENPRPGVIINKPDGEDVYKGVPKDYTKEAVNVQNFY 131

Query: 131 AAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVLKRKH 190
             +LGN+SGVTGG+GKV+ SGPND+IFIYY DHGA G++ MP GD + + D  +VL++ H
Sbjct: 132 NVLLGNESGVTGGNGKVVKSGPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLEKMH 191

Query: 191 EAKSYKSMVIYVEACEAGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPHTGYD 250
           + K Y  MVIYVEACE+GSMFEG+L +N+NIYA TA+N+ E SW  YCP   P P +   
Sbjct: 192 KRKKYNKMVIYVEACESGSMFEGILKKNLNIYAVTAANSKESSWGVYCPESYPPPPSEIG 251

Query: 251 TCLGDLYSVAWMEDS--------------------IGN------------------NFLN 310
           TCLGD +S++W+EDS                    +G+                  ++L+
Sbjct: 252 TCLGDTFSISWLEDSDLHDMSKETLEQQYHVVKRRVGSDVPETSHVCRFGTEKMLKDYLS 311

Query: 311 TYFGNTSDDNNNESSVKSTHTISSLSNGVSQRDASLLHYWYKFQNAPYGSREKMEASEQL 370
           +Y G  + +N+N +  +S  +  S S  V+ RD  LL+   K Q AP GS E  EA ++L
Sbjct: 312 SYIGR-NPENDNFTFTESFSSPISNSGLVNPRDIPLLYLQRKIQKAPMGSLESKEAQKKL 371

Query: 371 EDEILSRRHADNSINQIGELLFGEAKSSRVLNSIRGHGQSFVDDWNCFKKYVKIYEKHCK 421
            DE   R+  D SI  I  L   +     +L S R  GQ  VDDW+CFK  V  ++ HC 
Sbjct: 372 LDEKNHRKQIDQSITDILRLSVKQTNVLNLLTSTRTTGQPLVDDWDCFKTLVNSFKNHCG 431

BLAST of Csor.00g215520 vs. TAIR 10
Match: AT3G20210.2 (delta vacuolar processing enzyme )

HSP 1 Score: 402.5 bits (1033), Expect = 4.1e-112
Identity = 217/452 (48.01%), Postives = 278/452 (61.50%), Query Frame = 0

Query: 11  LLLIALLFFNSIIAKKQW-----VSHANTDQPGKRWALLVAGSNGYDNYRHQADVCHAYQ 70
           + L ALL F++   K Q      V  ++    G RWA+LVAGSN Y NYRHQAD+CHAYQ
Sbjct: 12  VFLHALLIFSAESRKTQLLNDNDVESSDKSAKGTRWAVLVAGSNEYYNYRHQADICHAYQ 71

Query: 71  ILRK-GIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNSVNFY 130
           ILRK G+ DENIIVFMYDDIA   +NPRPG+IINKP G DVY+GVPKDYT + VN  NFY
Sbjct: 72  ILRKGGLKDENIIVFMYDDIAFSSENPRPGVIINKPDGEDVYKGVPKDYTKEAVNVQNFY 131

Query: 131 AAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVLKRKH 190
             +LGN+SGVTGG+GKV+ SGPND+IFIYY DHGA G++ MP GD + + D  +VL++ H
Sbjct: 132 NVLLGNESGVTGGNGKVVKSGPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLEKMH 191

Query: 191 EAKSYKSMVIYVEACEAGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPHTGYD 250
           + K Y  MVIYVEACE+GSMFEG+L +N+NIYA TA+N+ E SW  YCP   P P +   
Sbjct: 192 KRKKYNKMVIYVEACESGSMFEGILKKNLNIYAVTAANSKESSWGVYCPESYPPPPSEIG 251

Query: 251 TCLGDLYSVAWMEDS--------------------IGN------------------NFLN 310
           TCLGD +S++W+EDS                    +G+                  ++L+
Sbjct: 252 TCLGDTFSISWLEDSDLHDMSKETLEQQYHVVKRRVGSDVPETSHVCRFGTEKMLKDYLS 311

Query: 311 TYFGNTSDDNNNESSVKSTHTISSLSNGVSQRDASLLHYWYKFQNAPYGSREKMEASEQL 370
           +Y G  + +N+N +  +S  +  S S  V+ RD  LL+   K Q AP GS E  EA ++L
Sbjct: 312 SYIGR-NPENDNFTFTESFSSPISNSGLVNPRDIPLLYLQRKIQKAPMGSLESKEAQKKL 371

Query: 371 EDEILSRRHADNSINQIGELLFGEAKSSRVLNSIRGHGQSFVDDWNCFKKYVKIYEKHCK 419
            DE   R+  D SI  I  L   +     +L S R  GQ  VDDW+CFK  V  ++ HC 
Sbjct: 372 LDEKNHRKQIDQSITDILRLSVKQTNVLNLLTSTRTTGQPLVDDWDCFKTLVNSFKNHCG 431

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P490443.0e-13154.57Vacuolar-processing enzyme OS=Vicia sativa OX=3908 PE=1 SV=1[more]
Q391197.3e-13054.01Vacuolar-processing enzyme gamma-isozyme OS=Arabidopsis thaliana OX=3702 GN=At4g... [more]
P490432.4e-12853.41Vacuolar-processing enzyme OS=Citrus sinensis OX=2711 PE=2 SV=1[more]
O243252.6e-12749.35Vacuolar-processing enzyme OS=Phaseolus vulgaris OX=3885 PE=1 SV=1[more]
P490476.4e-12651.86Vacuolar-processing enzyme alpha-isozyme OS=Arabidopsis thaliana OX=3702 GN=At2g... [more]
Match NameE-valueIdentityDescription
KAG6589920.10.0100.00hypothetical protein SDJN03_15343, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022961313.11.13e-30690.06vacuolar-processing enzyme gamma-isozyme-like [Cucurbita moschata][more]
XP_023516171.19.08e-30489.42vacuolar-processing enzyme gamma-isozyme-like [Cucurbita pepo subsp. pepo][more]
XP_022987711.11.01e-30088.55vacuolar-processing enzyme gamma-isozyme-like [Cucurbita maxima][more]
KAG7023589.12.58e-27390.65hypothetical protein SDJN02_14615, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
A0A6J1HBF55.47e-30790.06vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1JF374.91e-30188.55vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1D4751.28e-22667.74vacuolar-processing enzyme gamma-isozyme-like isoform X2 OS=Momordica charantia ... [more]
A0A6J1D2Z46.46e-22163.15vacuolar-processing enzyme-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A5A7TXA75.81e-22067.80Vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo var. makuwa OX=119... [more]
Match NameE-valueIdentityDescription
AT4G32940.15.2e-13154.01gamma vacuolar processing enzyme [more]
AT2G25940.14.6e-12751.86alpha-vacuolar processing enzyme [more]
AT1G62710.12.5e-11747.30beta vacuolar processing enzyme [more]
AT3G20210.14.9e-11348.02delta vacuolar processing enzyme [more]
AT3G20210.24.1e-11248.01delta vacuolar processing enzyme [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001096Peptidase C13, legumainPRINTSPR00776HEMOGLOBNASEcoord: 112..127
score: 50.54
coord: 140..159
score: 64.57
coord: 41..65
score: 75.48
coord: 189..205
score: 63.17
IPR001096Peptidase C13, legumainPIRSFPIRSF019663Legumaincoord: 258..424
e-value: 2.2E-43
score: 146.0
coord: 2..265
e-value: 6.9E-135
score: 447.8
IPR001096Peptidase C13, legumainPFAMPF01650Peptidase_C13coord: 40..263
e-value: 3.2E-95
score: 318.5
IPR001096Peptidase C13, legumainPANTHERPTHR12000HEMOGLOBINASE FAMILY MEMBERcoord: 262..421
IPR001096Peptidase C13, legumainPANTHERPTHR12000HEMOGLOBINASE FAMILY MEMBERcoord: 21..260
NoneNo IPR availableGENE3D1.10.132.130coord: 302..424
e-value: 2.9E-34
score: 119.9
NoneNo IPR availableGENE3D3.40.50.1460coord: 37..301
e-value: 1.1E-100
score: 338.4
NoneNo IPR availablePIRSRPIRSR019663-1PIRSR019663-1coord: 38..260
e-value: 1.6E-128
score: 426.8
NoneNo IPR availablePANTHERPTHR12000:SF45VACUOLAR-PROCESSING ENZYME-LIKEcoord: 262..421
NoneNo IPR availablePANTHERPTHR12000:SF45VACUOLAR-PROCESSING ENZYME-LIKEcoord: 21..260
IPR043577Asparaginyl endopeptidasePIRSFPIRSF500139AEcoord: 259..424
e-value: 8.4E-43
score: 144.2
coord: 3..268
e-value: 1.1E-135
score: 450.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g215520.m01Csor.00g215520.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004197 cysteine-type endopeptidase activity
molecular_function GO:0008233 peptidase activity