Csor.00g215360 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g215360
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	RNA methyltransferase
Location	Csor_Chr10: 3067948 .. 3068685 (-)
RNA-Seq Expression	Csor.00g215360
Synteny	Csor.00g215360

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGGTTGACGATGCTTACTGGAATCTAGGAATGGCAAGAATAGATTTTGGGAAGCGGGATAATTGTTTGGAGCTTCAAAGATAGAGACAGAAGCTTCTACAAGCATTTCTCTTCCAAAGAACATGATCTTCATACCGTTGTTTCCTTCCAACAGCAGAATTTTATATTTAGTCAATATCCACCTGAGAAGCACTATGATACAATTCTTTGGTAAGTTAATCTTTTTATCTTAGGTGTTGCGTATTAATGCTATTTGTTTTCTTGACCCTTGATGCGTCTCCCTTCCCCTTCTGGGGTCACAATTTGCGTTTCTTTTTTTCTTTCTGTACTGCAGTTTGAGTGTAGCAAAGTGGATTCATCAAAACTGGGGAGATGTTGGTTGGAGACTGCTTCCTCCGGTACTTACCTTGTCATACACCCATGTACTTTGTGTTAAATTGTCCTAAGATCATGAATAAGAACCTTCTGTATTTACTTTAATGTTTGATTAAGAACCTTCTTAATTATGCATTCATTTCAAAAAGGCAGGGTGGCCGCCAGGGTGGCATTCTTGTTTTGGAGCCTCCTTGGAAGTCTTATGAAGCCAACTATAGTGTTTCCGAGGTAAAAAATTTCATGCATCCAAATTGTTAATCAACAATTACATTTAGTAAAACAAAAAAAATTTTGAGTACTCTTCTAGCATCTGCTATCATATTGCCTTAGATTGCTGATCTTTCTAATGTTGACAACTAA

mRNA sequence

ATGCAGCAGAATTTTATATTTAGTCAATATCCACCTGAGAAGCACTATGATACAATTCTTTGTTTGAGTGTAGCAAAGTGGATTCATCAAAACTGGGGAGATGTTGGTTGGAGACTGCTTCCTCCGGGTGGCCGCCAGGGTGGCATTCTTGTTTTGGAGCCTCCTTGGAAGTCTTATGAAGCCAACTATAGTGTTTCCGAGATTGCTGATCTTTCTAATGTTGACAACTAA

Coding sequence (CDS)

ATGCAGCAGAATTTTATATTTAGTCAATATCCACCTGAGAAGCACTATGATACAATTCTTTGTTTGAGTGTAGCAAAGTGGATTCATCAAAACTGGGGAGATGTTGGTTGGAGACTGCTTCCTCCGGGTGGCCGCCAGGGTGGCATTCTTGTTTTGGAGCCTCCTTGGAAGTCTTATGAAGCCAACTATAGTGTTTCCGAGATTGCTGATCTTTCTAATGTTGACAACTAA

Protein sequence

MQQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEPPWKSYEANYSVSEIADLSNVDN

Homology

[Close]

BLAST of Csor.00g215360 vs. ExPASy Swiss-Prot
Match: Q6NPC9 (Probable RNA methyltransferase At5g51130 OS=Arabidopsis thaliana OX=3702 GN=At5g51130 PE=2 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 1.2e-14
Identity = 40/75 (53.33%), Postives = 48/75 (64.00%), Query Frame = 0


Query: 2   QQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVG--------WRLLPPGGRQGGILVLE 61
           ++NF+ ++   +  YDTILCLSV KW+H NWGD G        WRLL P    GGI V+E
Sbjct: 196 KENFVQTRNLDDNRYDTILCLSVTKWVHLNWGDDGLITLFSKIWRLLQP----GGIFVME 255

Query: 62  P-PWKSYEANYSVSE 68
           P PWKSYE N  VSE
Sbjct: 256 PQPWKSYENNRRVSE 266

[Close]

BLAST of Csor.00g215360 vs. ExPASy Swiss-Prot
Match: Q7L2J0 (7SK snRNA methylphosphate capping enzyme OS=Homo sapiens OX=9606 GN=MEPCE PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 5.7e-09
Identity = 29/57 (50.88%), Postives = 37/57 (64.91%), Query Frame = 0


Query: 16  YDTILCLSVAKWIHQNWGDVG----WRLLPPGGRQGGILVLEP-PWKSYEANYSVSE 68
           YD +LCLS+ KW+H NWGD G    +R +    R GGILVLEP PW SY    +++E
Sbjct: 575 YDVVLCLSLTKWVHLNWGDEGLKRMFRRIYRHLRPGGILVLEPQPWSSYGKRKTLTE 631

[Close]

BLAST of Csor.00g215360 vs. ExPASy Swiss-Prot
Match: A3KQ55 (7SK snRNA methylphosphate capping enzyme OS=Danio rerio OX=7955 GN=mepce PE=3 SV=2)

HSP 1 Score: 60.1 bits (144), Expect = 1.3e-08
Identity = 27/59 (45.76%), Postives = 35/59 (59.32%), Query Frame = 0


Query: 14  KHYDTILCLSVAKWIHQNWGDVG----WRLLPPGGRQGGILVLEP-PWKSYEANYSVSE 68
           + YD ILCLSV KW+H NWGD G    +  +    R GG+ +LEP PW SY     ++E
Sbjct: 530 EEYDVILCLSVTKWVHLNWGDAGLKRFFHRVYKHLRPGGLFILEPQPWSSYNKRKKLTE 588

[Close]

BLAST of Csor.00g215360 vs. ExPASy Swiss-Prot
Match: Q8K3A9 (7SK snRNA methylphosphate capping enzyme OS=Mus musculus OX=10090 GN=Mepce PE=1 SV=2)

HSP 1 Score: 60.1 bits (144), Expect = 1.3e-08
Identity = 29/57 (50.88%), Postives = 36/57 (63.16%), Query Frame = 0


Query: 16  YDTILCLSVAKWIHQNWGDVG----WRLLPPGGRQGGILVLEP-PWKSYEANYSVSE 68
           YD +LC S+ KW+H NWGD G    +R +    R GGILVLEP PW SY    S++E
Sbjct: 552 YDVVLCFSLTKWVHLNWGDEGLKRMFRRIYRHLRPGGILVLEPQPWSSYCKRKSLTE 608

[Close]

BLAST of Csor.00g215360 vs. ExPASy Swiss-Prot
Match: Q9U2R0 (Probable RNA methyltransferase Y17G7B.18 OS=Caenorhabditis elegans OX=6239 GN=Y17G7B.18 PE=3 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 2.2e-05
Identity = 27/64 (42.19%), Postives = 35/64 (54.69%), Query Frame = 0


Query: 13  EKHYDTILCLSVAKWIHQNWGDVGWR--------LLPPGGRQGGILVLEP-PWKSYEANY 68
           +  +D IL LS+ KWIH NWGD G R         L PGGR    L++EP  + SY+   
Sbjct: 254 QPEFDVILALSITKWIHLNWGDDGMRRFFRRAYAQLHPGGR----LIIEPQAFDSYKKRA 313

[Close]

BLAST of Csor.00g215360 vs. NCBI nr
Match: KAG6589936.1 (putative RNA methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 170 bits (430), Expect = 2.03e-53
Identity = 76/76 (100.00%), Postives = 76/76 (100.00%), Query Frame = 0


Query: 1  MQQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEPPWKSYE 60
          MQQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEPPWKSYE
Sbjct: 1  MQQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEPPWKSYE 60

Query: 61 ANYSVSEIADLSNVDN 76
          ANYSVSEIADLSNVDN
Sbjct: 61 ANYSVSEIADLSNVDN 76

[Close]

BLAST of Csor.00g215360 vs. NCBI nr
Match: XP_022987834.1 (probable RNA methyltransferase At5g51130 isoform X3 [Cucurbita maxima])

HSP 1 Score: 148 bits (374), Expect = 8.73e-43
Identity = 67/69 (97.10%), Postives = 67/69 (97.10%), Query Frame = 0


Query: 2   QQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEP-PWKSYE 61
           QQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEP PWKSYE
Sbjct: 148 QQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEPQPWKSYE 207

Query: 62  ANYSVSEIA 69
           ANYSVSE A
Sbjct: 208 ANYSVSEFA 216

[Close]

BLAST of Csor.00g215360 vs. NCBI nr
Match: XP_022987835.1 (probable RNA methyltransferase At5g51130 isoform X4 [Cucurbita maxima])

HSP 1 Score: 147 bits (370), Expect = 2.25e-42
Identity = 66/67 (98.51%), Postives = 66/67 (98.51%), Query Frame = 0


Query: 2   QQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEP-PWKSYE 61
           QQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEP PWKSYE
Sbjct: 148 QQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEPQPWKSYE 207

Query: 62  ANYSVSE 67
           ANYSVSE
Sbjct: 208 ANYSVSE 214

[Close]

BLAST of Csor.00g215360 vs. NCBI nr
Match: XP_022987833.1 (probable RNA methyltransferase At5g51130 isoform X2 [Cucurbita maxima])

HSP 1 Score: 147 bits (370), Expect = 3.71e-42
Identity = 66/67 (98.51%), Postives = 66/67 (98.51%), Query Frame = 0


Query: 2   QQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEP-PWKSYE 61
           QQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEP PWKSYE
Sbjct: 148 QQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEPQPWKSYE 207

Query: 62  ANYSVSE 67
           ANYSVSE
Sbjct: 208 ANYSVSE 214

[Close]

BLAST of Csor.00g215360 vs. NCBI nr
Match: XP_023516350.1 (probable RNA methyltransferase At5g51130 isoform X5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 145 bits (367), Expect = 6.97e-42
Identity = 66/70 (94.29%), Postives = 66/70 (94.29%), Query Frame = 0


Query: 2   QQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGR----QGGILVLEPPWK 61
           QQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGR    QGGILVLEPPWK
Sbjct: 148 QQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRLGGRQGGILVLEPPWK 207

Query: 62  SYEANYSVSE 67
           SYEANYSVSE
Sbjct: 208 SYEANYSVSE 217

[Close]

BLAST of Csor.00g215360 vs. ExPASy TrEMBL
Match: A0A6J1JJY3 (RNA methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111485259 PE=3 SV=1)

HSP 1 Score: 148 bits (374), Expect = 4.23e-43
Identity = 67/69 (97.10%), Postives = 67/69 (97.10%), Query Frame = 0


Query: 2   QQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEP-PWKSYE 61
           QQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEP PWKSYE
Sbjct: 148 QQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEPQPWKSYE 207

Query: 62  ANYSVSEIA 69
           ANYSVSE A
Sbjct: 208 ANYSVSEFA 216

[Close]

BLAST of Csor.00g215360 vs. ExPASy TrEMBL
Match: A0A6J1JHX9 (RNA methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111485259 PE=3 SV=1)

HSP 1 Score: 147 bits (370), Expect = 1.09e-42
Identity = 66/67 (98.51%), Postives = 66/67 (98.51%), Query Frame = 0


Query: 2   QQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEP-PWKSYE 61
           QQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEP PWKSYE
Sbjct: 148 QQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEPQPWKSYE 207

Query: 62  ANYSVSE 67
           ANYSVSE
Sbjct: 208 ANYSVSE 214

[Close]

BLAST of Csor.00g215360 vs. ExPASy TrEMBL
Match: A0A6J1JKK2 (RNA methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111485259 PE=3 SV=1)

HSP 1 Score: 147 bits (370), Expect = 1.80e-42
Identity = 66/67 (98.51%), Postives = 66/67 (98.51%), Query Frame = 0


Query: 2   QQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEP-PWKSYE 61
           QQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEP PWKSYE
Sbjct: 148 QQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEPQPWKSYE 207

Query: 62  ANYSVSE 67
           ANYSVSE
Sbjct: 208 ANYSVSE 214

[Close]

BLAST of Csor.00g215360 vs. ExPASy TrEMBL
Match: A0A6J1JFN8 (RNA methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111485308 PE=3 SV=1)

HSP 1 Score: 140 bits (353), Expect = 2.66e-41
Identity = 63/66 (95.45%), Postives = 63/66 (95.45%), Query Frame = 0


Query: 3   QNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEP-PWKSYEA 62
           Q FIFS YPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEP PWKSYEA
Sbjct: 38  QKFIFSPYPPEKHYDTILCLSVAKWIHQNWGDVGWRLLPPGGRQGGILVLEPQPWKSYEA 97

Query: 63  NYSVSE 67
           NYSVSE
Sbjct: 98  NYSVSE 103

[Close]

BLAST of Csor.00g215360 vs. ExPASy TrEMBL
Match: A0A6J1JBF8 (RNA methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111485259 PE=3 SV=1)

HSP 1 Score: 116 bits (291), Expect = 2.98e-30
Identity = 59/75 (78.67%), Postives = 59/75 (78.67%), Query Frame = 0


Query: 2   QQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVG--------WRLLPPGGRQGGILVLE 61
           QQNFIFSQYPPEKHYDTILCLSVAKWIH NWGD G        WRLL PGG    ILVLE
Sbjct: 148 QQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGDDGLITLFYKIWRLLHPGG----ILVLE 207

Query: 62  P-PWKSYEANYSVSE 67
           P PWKSYEANYSVSE
Sbjct: 208 PQPWKSYEANYSVSE 218

[Close]

BLAST of Csor.00g215360 vs. TAIR 10
Match: AT5G51130.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 80.1 bits (196), Expect = 8.5e-16
Identity = 40/75 (53.33%), Postives = 48/75 (64.00%), Query Frame = 0


Query: 2   QQNFIFSQYPPEKHYDTILCLSVAKWIHQNWGDVG--------WRLLPPGGRQGGILVLE 61
           ++NF+ ++   +  YDTILCLSV KW+H NWGD G        WRLL P    GGI V+E
Sbjct: 196 KENFVQTRNLDDNRYDTILCLSVTKWVHLNWGDDGLITLFSKIWRLLQP----GGIFVME 255

Query: 62  P-PWKSYEANYSVSE 68
           P PWKSYE N  VSE
Sbjct: 256 PQPWKSYENNRRVSE 266

The following BLAST results are available for this feature:

BLAST of Csor.00g215360 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NPC9	1.2e-14	53.33	Probable RNA methyltransferase At5g51130 OS=Arabidopsis thaliana OX=3702 GN=At5g...	[more]
Q7L2J0	5.7e-09	50.88	7SK snRNA methylphosphate capping enzyme OS=Homo sapiens OX=9606 GN=MEPCE PE=1 S...	[more]
A3KQ55	1.3e-08	45.76	7SK snRNA methylphosphate capping enzyme OS=Danio rerio OX=7955 GN=mepce PE=3 SV...	[more]
Q8K3A9	1.3e-08	50.88	7SK snRNA methylphosphate capping enzyme OS=Mus musculus OX=10090 GN=Mepce PE=1 ...	[more]
Q9U2R0	2.2e-05	42.19	Probable RNA methyltransferase Y17G7B.18 OS=Caenorhabditis elegans OX=6239 GN=Y1...	[more]

BLAST of Csor.00g215360 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6589936.1	2.03e-53	100.00	putative RNA methyltransferase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022987834.1	8.73e-43	97.10	probable RNA methyltransferase At5g51130 isoform X3 [Cucurbita maxima]	[more]
XP_022987835.1	2.25e-42	98.51	probable RNA methyltransferase At5g51130 isoform X4 [Cucurbita maxima]	[more]
XP_022987833.1	3.71e-42	98.51	probable RNA methyltransferase At5g51130 isoform X2 [Cucurbita maxima]	[more]
XP_023516350.1	6.97e-42	94.29	probable RNA methyltransferase At5g51130 isoform X5 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Csor.00g215360 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JJY3	4.23e-43	97.10	RNA methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111485259 PE=3 SV=1	[more]
A0A6J1JHX9	1.09e-42	98.51	RNA methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111485259 PE=3 SV=1	[more]
A0A6J1JKK2	1.80e-42	98.51	RNA methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111485259 PE=3 SV=1	[more]
A0A6J1JFN8	2.66e-41	95.45	RNA methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111485308 PE=3 SV=1	[more]
A0A6J1JBF8	2.98e-30	78.67	RNA methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111485259 PE=3 SV=1	[more]

BLAST of Csor.00g215360 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G51130.1	8.5e-16	53.33	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 1..74 e-value: 1.1E-10 score: 43.4
None	No IPR available	PANTHER	PTHR12315:SF2	BNAA03G13260D PROTEIN	coord: 3..71
IPR010675	RNA methyltransferase bin3, C-terminal	PFAM	PF06859	Bin3	coord: 16..67 e-value: 4.9E-11 score: 43.2
IPR039772	RNA methyltransferase Bin3-like	PANTHER	PTHR12315	BICOID-INTERACTING PROTEIN RELATED	coord: 3..71

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g215360.m01	Csor.00g215360.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001510	RNA methylation
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0008173	RNA methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity