Csor.00g214540 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g214540
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionThreonine synthase
LocationCsor_Chr10: 3518471 .. 3520479 (-)
RNA-Seq ExpressionCsor.00g214540
SyntenyCsor.00g214540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintronterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTACGCGTAGCCATGATGATCGACACGAGGTCACCCATCATCTGTCTCATAGCCAACACATTGGCTGGCCTCGAGCCCATGATGAGCCATGGATCCCAAAACCTTGCCACCATCCAACCAATGGTGTCGAGGTCTCAGCTTGGGCTCGAACACAGTCGACTGGTTCGTTGGAGAGCAAAGCTCATGGACACAAGGTTCACTAATTTGATAACCATGGACGTTGTACGGTTCGAACGCGGACGGTGGAGGGATGTTGGGAGTGGGCCTTCAGCGGGCCTTTTTTTCTTTGTAATCGTGCTCGTTTTGCTAGGTTAAAAGGAAGGTCTGGAAGTGGAGTTATAATCAGAGAAGCCTTGTTTTAAGACATTATCGCCGCCGCCGCCTCCGCCTCCGCCTCAGAATGGCTGCCTCTTCGCTGTTAAACCCCGCCATTTCTTTCCCCTCTTACAAGCCCAAGCTCCAAGCCAACCCAAAGTTCCGAAATCCCATTCTCGGAATCGGACGAGTATCCGCCACTTCCGATTCGTCTTCCTCCACGCCGACTAATGGCTCTCCGTCATTGTCGACCACCAAGAACCGCCGCTCTGCCGACGAGAACATCAAAGACGAAGCACGGCGCCATCGTGCAGCCTCCGGAGAAGACCGTAGCAACTTCTCGGCCAAGTACGTGCCATTTAACGCCGGCGCCGACTCGTCTGAGTGGTACTCGCTTGACGAGGTCGTTTACCGGAGCCGATCTGGTGGATTGCTCGACGTTCAGCACGACATGGAGGCCTTGAAGAAATTCGACGGCGCGTACTGGCGCAACCTCTTCGATTCACGCGTGGGGAAAACCACATGGCCTTATGGGTCCGGTGTGTGGAGCAAGAAGGAGTGGGTGTTGCCGGAGATTGACCCGGACGACATCGTCAGCGCCTTCGAAGGTAACTCAAACCTGTTTTGGGCTGAGAGATTTGGCAAACAGTTTCTGGGTATGAATGATTTATGGGTAAAACACTGTGGGATTAGCCACACTGGGAGTTTCAAGGATTTGGGCATGACTGTTTTGGTTAGCCAAGTGAACCGGCTGAGGAAATTGAAACGCCCCGTCGTCGGAGTCGGTTGTGCATCGACCGGAGACACATCGGCCGCGCTCTCAGCCTACTGCGCATCTGCAGGGATCCCTTCAATCGTCTTCCTCCCTGCAAACAAGATTTCCATGGCACAGTTAGTTCAGCCCATTGCAAATGGAGCCTTTGTGTTGAGTATAGACACTGATTTTGATGGCTGCATGAAGCTAATTAGGGAAGTTACAGCAGAATTGCCCATTTACTTAGCCAATTCCTTGAATAGTTTGAGGCTTGAAGGGCAAAAGACGGCTGCCATTGAAATTCTACAGCAGTTTGATTGGGCAGTACCTGAATGGGTGATCATTCCAGGAGGCAATCTTGGGAACATATATGCATTTTACAAAGGATTCAAGATGTGCCAAGAGCTTGGCCTTGTTGACAGGATTCCTAGGCTTGTTTGTGCACAAGCAGCCAATGCTAACCCTCTGTATTTACACTACAAATCTGGGTGGAAAGATTTCAAGGCTGTGAAAGCAAGTTCGACGTTCGCCTCGGCCATTCAGATTGGGGATCCTGTTTCCATAGATAGAGCTGTTTGTGCTCTACAGAATTCGAATGGCATCGTCGAGGAAGCGACAGAGGAGGAACTAATGGATGCAATGGCACAAGCTGACTCTACTGGGATGTTCATTTGCCCTCACACAGGGGTGGCACTAACAGCTTTGATCAAGCTGAGAAACCAAGGTGTCATCAGCCCGACCGATAGGACGGTGGTGGTGAGCACTGCTCATGGCCTGAAGTTTACACAGTCGAAAATCGACTATCACTCCAAGGAAATCCCAGACATGGCTTGCCAATTTGCTAACCCACCTGTTCAAGTGAAGGCTGATTATGGGAGTGTCATGGATGTACTTAAAGATTATTTACTACAAAAGTCTCCAATGTCTAAAGTGTAG

mRNA sequence

ATGATTACGCGTAGCCATGATGATCGACACGAGGTCACCCATCATCTGTCTCATAGCCAACACATTGGCTGGCCTCGAGCCCATGATGAGCCATGGATCCCAAAACCTTGCCACCATCCAACCAATGGTGTCGAGGTCTCAGCTTGGGCTCGAACACAGTCGACTGGTTCGTTGGAGAGCAAAGCTCATGGACACAAGGTTAAAAGGAAGGTCTGGAAGTGGAGTTATAATCAGAGAAGCCTTGTTTTAAGACATTATCGCCGCCGCCGCCTCCGCCTCCGCCTCAGAATGGCTGCCTCTTCGCTGTTAAACCCCGCCATTTCTTTCCCCTCTTACAAGCCCAAGCTCCAAGCCAACCCAAAGTTCCGAAATCCCATTCTCGGAATCGGACGAGTATCCGCCACTTCCGATTCGTCTTCCTCCACGCCGACTAATGGCTCTCCGTCATTGTCGACCACCAAGAACCGCCGCTCTGCCGACGAGAACATCAAAGACGAAGCACGGCGCCATCGTGCAGCCTCCGGAGAAGACCGTAGCAACTTCTCGGCCAAGTACGTGCCATTTAACGCCGGCGCCGACTCGTCTGAGTGGTACTCGCTTGACGAGGTCGTTTACCGGAGCCGATCTGGTGGATTGCTCGACGTTCAGCACGACATGGAGGCCTTGAAGAAATTCGACGGCGCGTACTGGCGCAACCTCTTCGATTCACGCGTGGGGAAAACCACATGGCCTTATGGGTCCGGTGTGTGGAGCAAGAAGGAGTGGGTGTTGCCGGAGATTGACCCGGACGACATCGTCAGCGCCTTCGAAGGTAACTCAAACCTGTTTTGGGCTGAGAGATTTGGCAAACAGTTTCTGGGTATGAATGATTTATGGGTAAAACACTGTGGGATTAGCCACACTGGGAGTTTCAAGGATTTGGGCATGACTGTTTTGGTTAGCCAAGTGAACCGGCTGAGGAAATTGAAACGCCCCGTCGTCGGAGTCGGTTGTGCATCGACCGGAGACACATCGGCCGCGCTCTCAGCCTACTGCGCATCTGCAGGGATCCCTTCAATCGTCTTCCTCCCTGCAAACAAGATTTCCATGGCACAGTTAGTTCAGCCCATTGCAAATGGAGCCTTTGTGTTGAGTATAGACACTGATTTTGATGGCTGCATGAAGCTAATTAGGGAAGTTACAGCAGAATTGCCCATTTACTTAGCCAATTCCTTGAATAGTTTGAGGCTTGAAGGGCAAAAGACGGCTGCCATTGAAATTCTACAGCAGTTTGATTGGGCAGTACCTGAATGGGTGATCATTCCAGGAGGCAATCTTGGGAACATATATGCATTTTACAAAGGATTCAAGATGTGCCAAGAGCTTGGCCTTGTTGACAGGATTCCTAGGCTTGTTTGTGCACAAGCAGCCAATGCTAACCCTCTGTATTTACACTACAAATCTGGGTGGAAAGATTTCAAGGCTGTGAAAGCAAGTTCGACGTTCGCCTCGGCCATTCAGATTGGGGATCCTGTTTCCATAGATAGAGCTGTTTGTGCTCTACAGAATTCGAATGGCATCGTCGAGGAAGCGACAGAGGAGGAACTAATGGATGCAATGGCACAAGCTGACTCTACTGGGATGTTCATTTGCCCTCACACAGGGGTGGCACTAACAGCTTTGATCAAGCTGAGAAACCAAGGTGTCATCAGCCCGACCGATAGGACGGTGGTGGTGAGCACTGCTCATGGCCTGAAGTTTACACAGTCGAAAATCGACTATCACTCCAAGGAAATCCCAGACATGGCTTGCCAATTTGCTAACCCACCTGTTCAAGTGAAGGCTGATTATGGGAGTGTCATGGATGTACTTAAAGATTATTTACTACAAAAGTCTCCAATGTCTAAAGTGTAG

Coding sequence (CDS)

ATGATTACGCGTAGCCATGATGATCGACACGAGGTCACCCATCATCTGTCTCATAGCCAACACATTGGCTGGCCTCGAGCCCATGATGAGCCATGGATCCCAAAACCTTGCCACCATCCAACCAATGGTGTCGAGGTCTCAGCTTGGGCTCGAACACAGTCGACTGGTTCGTTGGAGAGCAAAGCTCATGGACACAAGGTTAAAAGGAAGGTCTGGAAGTGGAGTTATAATCAGAGAAGCCTTGTTTTAAGACATTATCGCCGCCGCCGCCTCCGCCTCCGCCTCAGAATGGCTGCCTCTTCGCTGTTAAACCCCGCCATTTCTTTCCCCTCTTACAAGCCCAAGCTCCAAGCCAACCCAAAGTTCCGAAATCCCATTCTCGGAATCGGACGAGTATCCGCCACTTCCGATTCGTCTTCCTCCACGCCGACTAATGGCTCTCCGTCATTGTCGACCACCAAGAACCGCCGCTCTGCCGACGAGAACATCAAAGACGAAGCACGGCGCCATCGTGCAGCCTCCGGAGAAGACCGTAGCAACTTCTCGGCCAAGTACGTGCCATTTAACGCCGGCGCCGACTCGTCTGAGTGGTACTCGCTTGACGAGGTCGTTTACCGGAGCCGATCTGGTGGATTGCTCGACGTTCAGCACGACATGGAGGCCTTGAAGAAATTCGACGGCGCGTACTGGCGCAACCTCTTCGATTCACGCGTGGGGAAAACCACATGGCCTTATGGGTCCGGTGTGTGGAGCAAGAAGGAGTGGGTGTTGCCGGAGATTGACCCGGACGACATCGTCAGCGCCTTCGAAGGTAACTCAAACCTGTTTTGGGCTGAGAGATTTGGCAAACAGTTTCTGGGTATGAATGATTTATGGGTAAAACACTGTGGGATTAGCCACACTGGGAGTTTCAAGGATTTGGGCATGACTGTTTTGGTTAGCCAAGTGAACCGGCTGAGGAAATTGAAACGCCCCGTCGTCGGAGTCGGTTGTGCATCGACCGGAGACACATCGGCCGCGCTCTCAGCCTACTGCGCATCTGCAGGGATCCCTTCAATCGTCTTCCTCCCTGCAAACAAGATTTCCATGGCACAGTTAGTTCAGCCCATTGCAAATGGAGCCTTTGTGTTGAGTATAGACACTGATTTTGATGGCTGCATGAAGCTAATTAGGGAAGTTACAGCAGAATTGCCCATTTACTTAGCCAATTCCTTGAATAGTTTGAGGCTTGAAGGGCAAAAGACGGCTGCCATTGAAATTCTACAGCAGTTTGATTGGGCAGTACCTGAATGGGTGATCATTCCAGGAGGCAATCTTGGGAACATATATGCATTTTACAAAGGATTCAAGATGTGCCAAGAGCTTGGCCTTGTTGACAGGATTCCTAGGCTTGTTTGTGCACAAGCAGCCAATGCTAACCCTCTGTATTTACACTACAAATCTGGGTGGAAAGATTTCAAGGCTGTGAAAGCAAGTTCGACGTTCGCCTCGGCCATTCAGATTGGGGATCCTGTTTCCATAGATAGAGCTGTTTGTGCTCTACAGAATTCGAATGGCATCGTCGAGGAAGCGACAGAGGAGGAACTAATGGATGCAATGGCACAAGCTGACTCTACTGGGATGTTCATTTGCCCTCACACAGGGGTGGCACTAACAGCTTTGATCAAGCTGAGAAACCAAGGTGTCATCAGCCCGACCGATAGGACGGTGGTGGTGAGCACTGCTCATGGCCTGAAGTTTACACAGTCGAAAATCGACTATCACTCCAAGGAAATCCCAGACATGGCTTGCCAATTTGCTAACCCACCTGTTCAAGTGAAGGCTGATTATGGGAGTGTCATGGATGTACTTAAAGATTATTTACTACAAAAGTCTCCAATGTCTAAAGTGTAG

Protein sequence

MITRSHDDRHEVTHHLSHSQHIGWPRAHDEPWIPKPCHHPTNGVEVSAWARTQSTGSLESKAHGHKVKRKVWKWSYNQRSLVLRHYRRRRLRLRLRMAASSLLNPAISFPSYKPKLQANPKFRNPILGIGRVSATSDSSSSTPTNGSPSLSTTKNRRSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQHDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELGLVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAHGLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV
Homology
BLAST of Csor.00g214540 vs. ExPASy Swiss-Prot
Match: Q9MT28 (Threonine synthase, chloroplastic OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 868.2 bits (2242), Expect = 5.6e-251
Identity = 438/531 (82.49%), Postives = 471/531 (88.70%), Query Frame = 0

Query: 97  MAASSLLNPAISFPSYKPKL--QANPKFRNPILGIGRVSATSDSSSSTPTNGSPSLSTTK 156
           MAAS +L  +   P   P+L  Q+  K  N I     + AT+ +          ++S  K
Sbjct: 1   MAASCMLRSSFISPGL-PQLHHQSTSKPNNGIHFFTPIKATATND---------AISQQK 60

Query: 157 NRRSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLD 216
           +RR ADENI++EARRH ++      NFSA+YVPFNAG +S EWYSLDE+VYRSRSGGLLD
Sbjct: 61  HRRPADENIREEARRHCSS-----HNFSARYVPFNAGPNSDEWYSLDEIVYRSRSGGLLD 120

Query: 217 VQHDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSN 276
           VQHDM+ALKKFDG YWR+LFDSRVGKTTWPYGSGVWSKKEWVLPEID DDIVSAFEGNSN
Sbjct: 121 VQHDMDALKKFDGQYWRSLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSN 180

Query: 277 LFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCAST 336
           LFWAERFGKQFLGM DLWVKHCGISHTGSFKDLGMTVLVSQVNRLRK+ +PVVGVGCAST
Sbjct: 181 LFWAERFGKQFLGMTDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCAST 240

Query: 337 GDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVT 396
           GDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCM+LIREVT
Sbjct: 241 GDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMQLIREVT 300

Query: 397 AELPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQE 456
           AELPIYLANSLNSLRLEGQKTAAIEILQQFDW VPEWVI+PGGNLGNIYAFYKGF+MC+E
Sbjct: 301 AELPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPEWVIVPGGNLGNIYAFYKGFQMCKE 360

Query: 457 LGLVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCAL 516
           LGLVDRIPRLVCAQAANANPLYLHYKSGWKDFK VKA++TFASAIQIGDPVSIDRAV AL
Sbjct: 361 LGLVDRIPRLVCAQAANANPLYLHYKSGWKDFKPVKANTTFASAIQIGDPVSIDRAVFAL 420

Query: 517 QNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVST 576
           Q  NGIVEEATEEELMDAMAQADSTGMFICPHTGVALTAL KLRN GVI+PTDRTVVVST
Sbjct: 421 QQCNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALFKLRNSGVIAPTDRTVVVST 480

Query: 577 AHGLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKS 626
           AHGLKFTQSKIDYHSKEI DM C+FANPPV+VKAD+GSVMDVLK YLL ++
Sbjct: 481 AHGLKFTQSKIDYHSKEIKDMECRFANPPVEVKADFGSVMDVLKSYLLSQN 516

BLAST of Csor.00g214540 vs. ExPASy Swiss-Prot
Match: Q9S7B5 (Threonine synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS1 PE=1 SV=1)

HSP 1 Score: 852.0 bits (2200), Expect = 4.1e-246
Identity = 431/528 (81.63%), Postives = 468/528 (88.64%), Query Frame = 0

Query: 97  MAASSLLNPAISFPSYKPKLQANPKFRNPILGIGR---VSATSDSSSSTPTNGSPSLSTT 156
           MA+S L N ++S  S  PK     + R+  L   R   +S T+D ++      +P  +  
Sbjct: 1   MASSCLFNASVS--SLNPKQDPIRRHRSTSLLRHRPVVISCTADGNNIK----APIETAV 60

Query: 157 KNRRSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLL 216
           K     ++NI+DEARR+R+ +    + FSAKYVPFNA   S+E YSLDE+VYRSRSGGLL
Sbjct: 61  KPPHRTEDNIRDEARRNRSNA---VNPFSAKYVPFNAAPGSTESYSLDEIVYRSRSGGLL 120

Query: 217 DVQHDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNS 276
           DV+HDMEALK+FDGAYWR+LFDSRVGK+TWPYGSGVWSKKEWVLPEID DDIVSAFEGNS
Sbjct: 121 DVEHDMEALKRFDGAYWRDLFDSRVGKSTWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNS 180

Query: 277 NLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCAS 336
           NLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRK+KRPVVGVGCAS
Sbjct: 181 NLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMKRPVVGVGCAS 240

Query: 337 TGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREV 396
           TGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIRE+
Sbjct: 241 TGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREI 300

Query: 397 TAELPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQ 456
           TAELPIYLANSLNSLRLEGQKTAAIEILQQFDW VP+WVI+PGGNLGNIYAFYKGFKMCQ
Sbjct: 301 TAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFKMCQ 360

Query: 457 ELGLVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCA 516
           ELGLVDRIPR+VCAQAANANPLYLHYKSGWKDFK + AS+TFASAIQIGDPVSIDRAV A
Sbjct: 361 ELGLVDRIPRMVCAQAANANPLYLHYKSGWKDFKPMTASTTFASAIQIGDPVSIDRAVYA 420

Query: 517 LQNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVS 576
           L+  NGIVEEATEEELMDAMAQADSTGMFICPHTGVALTAL KLRNQGVI+PTDRTVVVS
Sbjct: 421 LKKCNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALFKLRNQGVIAPTDRTVVVS 480

Query: 577 TAHGLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYL 622
           TAHGLKFTQSKIDYHS  IPDMAC+F+NPPV VKAD+G+VMDVLK YL
Sbjct: 481 TAHGLKFTQSKIDYHSNAIPDMACRFSNPPVDVKADFGAVMDVLKSYL 519

BLAST of Csor.00g214540 vs. ExPASy Swiss-Prot
Match: Q9SSP5 (Threonine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS2 PE=1 SV=1)

HSP 1 Score: 798.9 bits (2062), Expect = 4.2e-230
Identity = 409/526 (77.76%), Postives = 446/526 (84.79%), Query Frame = 0

Query: 97  MAASSLLNPAISFPSYKPKLQANPKFRNPILGIGRVSATSDSSSSTPTNGSPSLSTTKNR 156
           MA+ SL + A  FPS+             +      S T   +S++P    P  +  K R
Sbjct: 1   MASFSLPHSATYFPSHS---------ETSLKPHSAASFTVRCTSASP--AVPPQTPQKPR 60

Query: 157 RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 216
           RS DENI+DEARR          N SA+YVPFNA   S+E YSLDE+VYRS+SG LLDVQ
Sbjct: 61  RSPDENIRDEARRR----PHQLQNLSARYVPFNAPPSSTESYSLDEIVYRSQSGALLDVQ 120

Query: 217 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 276
           HD  ALK++DG +WRNLFDSRVGKT WPYGSGVWSKKEWVLPEID DDIVSAFEGNSNLF
Sbjct: 121 HDFAALKRYDGEFWRNLFDSRVGKTNWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNLF 180

Query: 277 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 336
           WAERFGKQ+L MNDLWVKHCGISHTGSFKDLGM+VLVSQVNRLRK+ +PV+GVGCASTGD
Sbjct: 181 WAERFGKQYLQMNDLWVKHCGISHTGSFKDLGMSVLVSQVNRLRKMNKPVIGVGCASTGD 240

Query: 337 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 396
           TSAALSAYCASAGIPSIVFLPA+KISMAQLVQPIANGAFVLSIDTDFDGCM LIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPADKISMAQLVQPIANGAFVLSIDTDFDGCMHLIREVTAE 300

Query: 397 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 456
           LPIYLANSLNSLRLEGQKTAAIEILQQF+W VP+WVI+PGGNLGNIYAFYKGF MC+ELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFNWQVPDWVIVPGGNLGNIYAFYKGFHMCKELG 360

Query: 457 LVDRIPRLVCAQAANANPLYLHYKSGWK-DFKAVKASSTFASAIQIGDPVSIDRAVCALQ 516
           LVDRIPRLVCAQAANANPLYLHYKSG+K DF  +KA++TFASAIQIGDPVSIDRAV AL+
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGFKEDFNPLKANTTFASAIQIGDPVSIDRAVYALK 420

Query: 517 NSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTA 576
            SNGIVEEATEEELMDA A ADSTGMFICPHTGVALTAL+KLR  GVI   DRTVVVSTA
Sbjct: 421 KSNGIVEEATEEELMDATALADSTGMFICPHTGVALTALMKLRKSGVIGANDRTVVVSTA 480

Query: 577 HGLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYL 622
           HGLKFTQSKIDYHSK I +MAC+ ANPPV+VKA +GSVMDVLK+YL
Sbjct: 481 HGLKFTQSKIDYHSKNIKEMACRLANPPVKVKAKFGSVMDVLKEYL 511

BLAST of Csor.00g214540 vs. ExPASy Swiss-Prot
Match: Q58860 (Threonine synthase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=thrC PE=3 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 6.1e-72
Identity = 157/423 (37.12%), Postives = 242/423 (57.21%), Query Frame = 0

Query: 198 YSLDEVVYRSRSGGLLDVQHDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVL 257
           Y +DE++Y    GGLL++ +D E +K         + + ++ K       GVW   E+ L
Sbjct: 12  YDVDEIIYTCECGGLLEIIYDYEEIKD-------KVSEEKLRKRE----IGVWRYLEY-L 71

Query: 258 PEIDPDDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVN 317
           P  D   IVS  EG + L+      K+ LG+ +L+VK+ G + TGSFKD GMTV V++ N
Sbjct: 72  PVKDESKIVSLCEGGTPLYRCNNLEKE-LGIKELYVKNEGANPTGSFKDRGMTVGVTRAN 131

Query: 318 RLRKLKRPVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVL 377
            L      V  VGCASTG+TSA+L+AY A +G   IV LP  K+++ +L Q +  GA V+
Sbjct: 132 EL-----GVEVVGCASTGNTSASLAAYSARSGKKCIVLLPEGKVALGKLAQAMFYGAKVI 191

Query: 378 SIDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGG 437
            +  +FD  + +++++  E  IYL NS+N  RLEGQKT A EI  Q +W VP+ VI+P G
Sbjct: 192 QVKGNFDDALDMVKQLAKEKLIYLLNSINPFRLEGQKTIAFEICDQLNWQVPDRVIVPVG 251

Query: 438 NLGNIYAFYKGFKMCQELGLVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFAS 497
           N GNI A +KGFK  +  G++D +P++   QA  A P+   ++   KD    K   T A+
Sbjct: 252 NAGNISAIWKGFKEFEITGIIDELPKMTGIQADGAKPIVEAFRKRAKDIIPYKNPETIAT 311

Query: 498 AIQIGDPVSIDRAVCALQNSNGIVEEATEEELMDAM-AQADSTGMFICPHTGVALTALIK 557
           AI+IG+PV+  +A+ A+ +S G  E  T+EE+++A    A   G+F+ P +  ++  L K
Sbjct: 312 AIRIGNPVNAPKALDAIYSSGGYAEAVTDEEIVEAQKLLARKEGIFVEPASASSIAGLKK 371

Query: 558 LRNQGVISPTDRTVVVSTAHGLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDV 617
           L  +G+I   +R V ++T HGLK             PD A + +  P++++ D   +  +
Sbjct: 372 LLEEGIIDRDERIVCITTGHGLK------------DPDAAIRASEEPIKIECDMNVLKRI 404

Query: 618 LKD 620
           LK+
Sbjct: 432 LKE 404

BLAST of Csor.00g214540 vs. ExPASy Swiss-Prot
Match: Q8TQD4 (Threonine synthase OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) OX=188937 GN=thrC PE=1 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 9.4e-65
Identity = 152/391 (38.87%), Postives = 227/391 (58.06%), Query Frame = 0

Query: 198 YSLDEVVYR-SRSGGLLDVQHDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWV 257
           YS DEV+Y  S+  GLLDV +D  ++K           D    KT  P    VW   + +
Sbjct: 13  YSRDEVIYTCSKCDGLLDVIYDYSSIK----------IDMEKLKTECP---SVWKYAKLL 72

Query: 258 LPEIDPDDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQV 317
             E +P   V+  EG + L+  +R  ++ +G+  L+VKH G++ TGSFKD GMTV V+  
Sbjct: 73  PVEREP---VTIQEGGTPLYKCDRLAEK-IGIKKLYVKHEGMNPTGSFKDRGMTVGVT-- 132

Query: 318 NRLRKLKRPVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFV 377
              + L+  +  V CASTG+TSAAL+ Y A AGIP +V LPA K+++ ++ Q + +GA V
Sbjct: 133 ---KALELGMNTVACASTGNTSAALAIYGAKAGIPVVVLLPAGKVALGKVAQALMHGAKV 192

Query: 378 LSIDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPG 437
           LSI  +FD  + L+R + ++  IYL NS+N  RLEGQKT   EI  Q D+ VP+ +++P 
Sbjct: 193 LSIRGNFDDALALVRTLCSQEKIYLLNSINPYRLEGQKTIGFEIADQLDFKVPDRIVLPV 252

Query: 438 GNLGNIYAFYKGFKMCQELGLVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFA 497
           GN GNI A YKGF+  + LG+ D +P++   QA  + P+    KSG       +   T A
Sbjct: 253 GNAGNITAIYKGFREFKILGITDSLPKMTGIQAEGSCPIVKAIKSGAPAITPEENPETVA 312

Query: 498 SAIQIGDPVSIDRAVCALQNSNGIVEEATEEELMDAMAQ-ADSTGMFICPHTGVALTALI 557
           +AI+IG+PV+  +A+ A++ S G  E  T+EE++ A    A   G+ + P +  ++  L 
Sbjct: 313 TAIRIGNPVNATKALSAIRESGGTAESVTDEEILAAQKDLARLEGIGVEPASAASVAGLR 372

Query: 558 KLRNQGVISPTDRTVVVSTAHGLKFTQSKID 587
           KL + GVI   +  V ++T H LK  Q+ ID
Sbjct: 373 KLVDMGVIGRDETVVCITTGHLLKDPQTVID 381

BLAST of Csor.00g214540 vs. NCBI nr
Match: KAG6590018.1 (hypothetical protein SDJN03_15441, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1273 bits (3294), Expect = 0.0
Identity = 630/630 (100.00%), Postives = 630/630 (100.00%), Query Frame = 0

Query: 1   MITRSHDDRHEVTHHLSHSQHIGWPRAHDEPWIPKPCHHPTNGVEVSAWARTQSTGSLES 60
           MITRSHDDRHEVTHHLSHSQHIGWPRAHDEPWIPKPCHHPTNGVEVSAWARTQSTGSLES
Sbjct: 1   MITRSHDDRHEVTHHLSHSQHIGWPRAHDEPWIPKPCHHPTNGVEVSAWARTQSTGSLES 60

Query: 61  KAHGHKVKRKVWKWSYNQRSLVLRHYRRRRLRLRLRMAASSLLNPAISFPSYKPKLQANP 120
           KAHGHKVKRKVWKWSYNQRSLVLRHYRRRRLRLRLRMAASSLLNPAISFPSYKPKLQANP
Sbjct: 61  KAHGHKVKRKVWKWSYNQRSLVLRHYRRRRLRLRLRMAASSLLNPAISFPSYKPKLQANP 120

Query: 121 KFRNPILGIGRVSATSDSSSSTPTNGSPSLSTTKNRRSADENIKDEARRHRAASGEDRSN 180
           KFRNPILGIGRVSATSDSSSSTPTNGSPSLSTTKNRRSADENIKDEARRHRAASGEDRSN
Sbjct: 121 KFRNPILGIGRVSATSDSSSSTPTNGSPSLSTTKNRRSADENIKDEARRHRAASGEDRSN 180

Query: 181 FSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQHDMEALKKFDGAYWRNLFDSRVGK 240
           FSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQHDMEALKKFDGAYWRNLFDSRVGK
Sbjct: 181 FSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQHDMEALKKFDGAYWRNLFDSRVGK 240

Query: 241 TTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISH 300
           TTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISH
Sbjct: 241 TTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISH 300

Query: 301 TGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANK 360
           TGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANK
Sbjct: 301 TGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANK 360

Query: 361 ISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEI 420
           ISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEI
Sbjct: 361 ISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEI 420

Query: 421 LQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELGLVDRIPRLVCAQAANANPLYLHYK 480
           LQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELGLVDRIPRLVCAQAANANPLYLHYK
Sbjct: 421 LQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELGLVDRIPRLVCAQAANANPLYLHYK 480

Query: 481 SGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQNSNGIVEEATEEELMDAMAQADSTG 540
           SGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQNSNGIVEEATEEELMDAMAQADSTG
Sbjct: 481 SGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQNSNGIVEEATEEELMDAMAQADSTG 540

Query: 541 MFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAHGLKFTQSKIDYHSKEIPDMACQFA 600
           MFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAHGLKFTQSKIDYHSKEIPDMACQFA
Sbjct: 541 MFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAHGLKFTQSKIDYHSKEIPDMACQFA 600

Query: 601 NPPVQVKADYGSVMDVLKDYLLQKSPMSKV 630
           NPPVQVKADYGSVMDVLKDYLLQKSPMSKV
Sbjct: 601 NPPVQVKADYGSVMDVLKDYLLQKSPMSKV 630

BLAST of Csor.00g214540 vs. NCBI nr
Match: XP_022960888.1 (threonine synthase, chloroplastic [Cucurbita moschata] >KAG7023681.1 hypothetical protein SDJN02_14707, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1066 bits (2756), Expect = 0.0
Identity = 533/534 (99.81%), Postives = 533/534 (99.81%), Query Frame = 0

Query: 97  MAASSLLNPAISFPSYKPKLQANPKFRNPILGIGRVSATSDSSSSTPTNGSPSLSTTKNR 156
           MAASSLLNPAISFPSYKPKLQANPKFRNPIL IGRVSATSDSSSSTPTNGSPSLSTTKNR
Sbjct: 1   MAASSLLNPAISFPSYKPKLQANPKFRNPILRIGRVSATSDSSSSTPTNGSPSLSTTKNR 60

Query: 157 RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 216
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120

Query: 217 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 276
           HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 277 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 336
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 337 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 396
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 397 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 456
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360

Query: 457 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 516
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420

Query: 517 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 576
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480

Query: 577 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 630
           GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV
Sbjct: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 534

BLAST of Csor.00g214540 vs. NCBI nr
Match: XP_022987673.1 (threonine synthase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1057 bits (2734), Expect = 0.0
Identity = 529/534 (99.06%), Postives = 530/534 (99.25%), Query Frame = 0

Query: 97  MAASSLLNPAISFPSYKPKLQANPKFRNPILGIGRVSATSDSSSSTPTNGSPSLSTTKNR 156
           MAASSLLNPAISFPSYKPKLQANP FRNPIL I R SATSDSSSSTPTNGSPSLSTTKNR
Sbjct: 1   MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSATSDSSSSTPTNGSPSLSTTKNR 60

Query: 157 RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 216
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120

Query: 217 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 276
           HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 277 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 336
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 337 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 396
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 397 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 456
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360

Query: 457 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 516
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420

Query: 517 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 576
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480

Query: 577 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 630
           GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQK+PMSKV
Sbjct: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKAPMSKV 534

BLAST of Csor.00g214540 vs. NCBI nr
Match: XP_023515876.1 (threonine synthase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1056 bits (2730), Expect = 0.0
Identity = 528/534 (98.88%), Postives = 530/534 (99.25%), Query Frame = 0

Query: 97  MAASSLLNPAISFPSYKPKLQANPKFRNPILGIGRVSATSDSSSSTPTNGSPSLSTTKNR 156
           MAASSLLNPAISFPSYKPKLQANP FRNPIL I R SA+SDSSSSTPTNGSPSLSTTKNR
Sbjct: 1   MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSASSDSSSSTPTNGSPSLSTTKNR 60

Query: 157 RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 216
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120

Query: 217 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 276
           HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 277 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 336
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 337 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 396
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 397 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 456
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360

Query: 457 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 516
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420

Query: 517 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 576
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480

Query: 577 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 630
           GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQK+PMSKV
Sbjct: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKAPMSKV 534

BLAST of Csor.00g214540 vs. NCBI nr
Match: XP_038878968.1 (threonine synthase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 1024 bits (2647), Expect = 0.0
Identity = 507/534 (94.94%), Postives = 519/534 (97.19%), Query Frame = 0

Query: 97  MAASSLLNPAISFPSYKPKLQANPKFRNPILGIGRVSATSDSSSSTPTNGSPSLSTTKNR 156
           MA SSLLNPAISFPS KPKLQ NPKFRNP+L I + S+TSDSSSS PTNGSP LST KNR
Sbjct: 1   MATSSLLNPAISFPSRKPKLQPNPKFRNPVLRIPKASSTSDSSSSPPTNGSPPLSTLKNR 60

Query: 157 RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 216
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADS EWYSLDE++YRSRSGGLLDVQ
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSPEWYSLDEIIYRSRSGGLLDVQ 120

Query: 217 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 276
           HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 277 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 336
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 337 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 396
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 397 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 456
           LPIYLANSLNSLRLEGQKTAAIEILQQFDW VPEWVI+PGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELG 360

Query: 457 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 516
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAV ALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQN 420

Query: 517 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 576
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTAL+KLRN+G+I PTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALVKLRNKGIIRPTDRTVVVSTAH 480

Query: 577 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 630
           GLKFTQSKIDYHSK+IPDMACQFANPPVQVKADYGSVMDVLKDYLL K+P S+V
Sbjct: 481 GLKFTQSKIDYHSKDIPDMACQFANPPVQVKADYGSVMDVLKDYLLNKAPKSQV 534

BLAST of Csor.00g214540 vs. ExPASy TrEMBL
Match: A0A6J1HAA4 (Threonine synthase OS=Cucurbita moschata OX=3662 GN=LOC111461551 PE=3 SV=1)

HSP 1 Score: 1066 bits (2756), Expect = 0.0
Identity = 533/534 (99.81%), Postives = 533/534 (99.81%), Query Frame = 0

Query: 97  MAASSLLNPAISFPSYKPKLQANPKFRNPILGIGRVSATSDSSSSTPTNGSPSLSTTKNR 156
           MAASSLLNPAISFPSYKPKLQANPKFRNPIL IGRVSATSDSSSSTPTNGSPSLSTTKNR
Sbjct: 1   MAASSLLNPAISFPSYKPKLQANPKFRNPILRIGRVSATSDSSSSTPTNGSPSLSTTKNR 60

Query: 157 RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 216
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120

Query: 217 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 276
           HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 277 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 336
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 337 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 396
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 397 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 456
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360

Query: 457 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 516
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420

Query: 517 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 576
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480

Query: 577 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 630
           GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV
Sbjct: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 534

BLAST of Csor.00g214540 vs. ExPASy TrEMBL
Match: A0A6J1JK49 (Threonine synthase OS=Cucurbita maxima OX=3661 GN=LOC111485156 PE=3 SV=1)

HSP 1 Score: 1057 bits (2734), Expect = 0.0
Identity = 529/534 (99.06%), Postives = 530/534 (99.25%), Query Frame = 0

Query: 97  MAASSLLNPAISFPSYKPKLQANPKFRNPILGIGRVSATSDSSSSTPTNGSPSLSTTKNR 156
           MAASSLLNPAISFPSYKPKLQANP FRNPIL I R SATSDSSSSTPTNGSPSLSTTKNR
Sbjct: 1   MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSATSDSSSSTPTNGSPSLSTTKNR 60

Query: 157 RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 216
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120

Query: 217 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 276
           HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 277 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 336
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 337 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 396
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 397 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 456
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360

Query: 457 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 516
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420

Query: 517 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 576
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480

Query: 577 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 630
           GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQK+PMSKV
Sbjct: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKAPMSKV 534

BLAST of Csor.00g214540 vs. ExPASy TrEMBL
Match: A0A5D3CDZ3 (Threonine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001840 PE=3 SV=1)

HSP 1 Score: 1003 bits (2592), Expect = 0.0
Identity = 500/534 (93.63%), Postives = 512/534 (95.88%), Query Frame = 0

Query: 97  MAASSLLNPAISFPSYKPKLQANPKFRNPILGIGRVSATSDSSSSTPTNGSPSLSTTKNR 156
           MA SSL NP ISFPS  PKLQ+ PKFRNPIL I +  ATSDSS S PTNGSP LST KNR
Sbjct: 1   MATSSLFNPTISFPSRNPKLQSTPKFRNPILRIPK--ATSDSSPSPPTNGSPPLSTLKNR 60

Query: 157 RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 216
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAG DS+E YSLDE+VYRSRSGGLLD+Q
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQ 120

Query: 217 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 276
           HDMEALKK+DGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 277 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 336
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 337 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 396
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 397 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 456
           LPIYLANSLNSLRLEGQKTAAIEILQQFDW VPEWVI+PGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELG 360

Query: 457 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 516
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRA+ ALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAIYALQN 420

Query: 517 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 576
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRN+G+I PTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAH 480

Query: 577 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 630
           GLKF QSKIDYHSKEIPDMACQFANPPVQVKAD+GSVMDVLKDYLL K+P SKV
Sbjct: 481 GLKFAQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNKAPKSKV 532

BLAST of Csor.00g214540 vs. ExPASy TrEMBL
Match: A0A5A7UKY2 (Threonine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001890 PE=3 SV=1)

HSP 1 Score: 1001 bits (2587), Expect = 0.0
Identity = 500/534 (93.63%), Postives = 511/534 (95.69%), Query Frame = 0

Query: 97  MAASSLLNPAISFPSYKPKLQANPKFRNPILGIGRVSATSDSSSSTPTNGSPSLSTTKNR 156
           MA  SL NP ISFPS  PKLQ+ PKFRNPIL I +  ATSDSS S PTNGSP LST KNR
Sbjct: 1   MATYSLFNPTISFPSRNPKLQSTPKFRNPILRIPK--ATSDSSPSPPTNGSPPLSTLKNR 60

Query: 157 RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 216
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAG DS+E YSLDE+VYRSRSGGLLD+Q
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQ 120

Query: 217 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 276
           HDMEALKK+DGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 277 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 336
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 337 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 396
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 397 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 456
           LPIYLANSLNSLRLEGQKTAAIEILQQFDW VPEWVI+PGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELG 360

Query: 457 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 516
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAV ALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQN 420

Query: 517 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 576
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRN+G+I PTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAH 480

Query: 577 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 630
           GLKF QSKIDYHSKEIPDMACQFANPPVQVKAD+GSVMDVLKDYLL K+P SKV
Sbjct: 481 GLKFAQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNKAPKSKV 532

BLAST of Csor.00g214540 vs. ExPASy TrEMBL
Match: A0A0A0M223 (Threonine synthase OS=Cucumis sativus OX=3659 GN=Csa_1G590270 PE=3 SV=1)

HSP 1 Score: 1001 bits (2587), Expect = 0.0
Identity = 500/534 (93.63%), Postives = 511/534 (95.69%), Query Frame = 0

Query: 97  MAASSLLNPAISFPSYKPKLQANPKFRNPILGIGRVSATSDSSSSTPTNGSPSLSTTKNR 156
           MA SSL NP IS PS  PKLQ+ PKFRNPIL I +  ATSDSS S PTNGSP LST KNR
Sbjct: 1   MATSSLFNPTISLPSRNPKLQSTPKFRNPILRIPK--ATSDSSPSPPTNGSPPLSTLKNR 60

Query: 157 RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 216
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAG DS+E YSLDE+VYRSRSGGLLD+Q
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQ 120

Query: 217 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 276
           HDMEALKK+DGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 277 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 336
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 337 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 396
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 397 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 456
           LPIYLANSLNSLRLEGQKTAAIEILQQFDW VPEWVI+PGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELG 360

Query: 457 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 516
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAV ALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQN 420

Query: 517 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 576
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRN+G+I PTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAH 480

Query: 577 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 630
           GLKFTQSKIDYHSKEIPDMACQFANPPVQVKAD+GSVMDVLKDYLL  +P SKV
Sbjct: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNSAPKSKV 532

BLAST of Csor.00g214540 vs. TAIR 10
Match: AT4G29840.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 852.0 bits (2200), Expect = 2.9e-247
Identity = 431/528 (81.63%), Postives = 468/528 (88.64%), Query Frame = 0

Query: 97  MAASSLLNPAISFPSYKPKLQANPKFRNPILGIGR---VSATSDSSSSTPTNGSPSLSTT 156
           MA+S L N ++S  S  PK     + R+  L   R   +S T+D ++      +P  +  
Sbjct: 1   MASSCLFNASVS--SLNPKQDPIRRHRSTSLLRHRPVVISCTADGNNIK----APIETAV 60

Query: 157 KNRRSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLL 216
           K     ++NI+DEARR+R+ +    + FSAKYVPFNA   S+E YSLDE+VYRSRSGGLL
Sbjct: 61  KPPHRTEDNIRDEARRNRSNA---VNPFSAKYVPFNAAPGSTESYSLDEIVYRSRSGGLL 120

Query: 217 DVQHDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNS 276
           DV+HDMEALK+FDGAYWR+LFDSRVGK+TWPYGSGVWSKKEWVLPEID DDIVSAFEGNS
Sbjct: 121 DVEHDMEALKRFDGAYWRDLFDSRVGKSTWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNS 180

Query: 277 NLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCAS 336
           NLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRK+KRPVVGVGCAS
Sbjct: 181 NLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMKRPVVGVGCAS 240

Query: 337 TGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREV 396
           TGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIRE+
Sbjct: 241 TGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREI 300

Query: 397 TAELPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQ 456
           TAELPIYLANSLNSLRLEGQKTAAIEILQQFDW VP+WVI+PGGNLGNIYAFYKGFKMCQ
Sbjct: 301 TAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFKMCQ 360

Query: 457 ELGLVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCA 516
           ELGLVDRIPR+VCAQAANANPLYLHYKSGWKDFK + AS+TFASAIQIGDPVSIDRAV A
Sbjct: 361 ELGLVDRIPRMVCAQAANANPLYLHYKSGWKDFKPMTASTTFASAIQIGDPVSIDRAVYA 420

Query: 517 LQNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVS 576
           L+  NGIVEEATEEELMDAMAQADSTGMFICPHTGVALTAL KLRNQGVI+PTDRTVVVS
Sbjct: 421 LKKCNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALFKLRNQGVIAPTDRTVVVS 480

Query: 577 TAHGLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYL 622
           TAHGLKFTQSKIDYHS  IPDMAC+F+NPPV VKAD+G+VMDVLK YL
Sbjct: 481 TAHGLKFTQSKIDYHSNAIPDMACRFSNPPVDVKADFGAVMDVLKSYL 519

BLAST of Csor.00g214540 vs. TAIR 10
Match: AT1G72810.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 798.9 bits (2062), Expect = 3.0e-231
Identity = 409/526 (77.76%), Postives = 446/526 (84.79%), Query Frame = 0

Query: 97  MAASSLLNPAISFPSYKPKLQANPKFRNPILGIGRVSATSDSSSSTPTNGSPSLSTTKNR 156
           MA+ SL + A  FPS+             +      S T   +S++P    P  +  K R
Sbjct: 1   MASFSLPHSATYFPSHS---------ETSLKPHSAASFTVRCTSASP--AVPPQTPQKPR 60

Query: 157 RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 216
           RS DENI+DEARR          N SA+YVPFNA   S+E YSLDE+VYRS+SG LLDVQ
Sbjct: 61  RSPDENIRDEARRR----PHQLQNLSARYVPFNAPPSSTESYSLDEIVYRSQSGALLDVQ 120

Query: 217 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 276
           HD  ALK++DG +WRNLFDSRVGKT WPYGSGVWSKKEWVLPEID DDIVSAFEGNSNLF
Sbjct: 121 HDFAALKRYDGEFWRNLFDSRVGKTNWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNLF 180

Query: 277 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 336
           WAERFGKQ+L MNDLWVKHCGISHTGSFKDLGM+VLVSQVNRLRK+ +PV+GVGCASTGD
Sbjct: 181 WAERFGKQYLQMNDLWVKHCGISHTGSFKDLGMSVLVSQVNRLRKMNKPVIGVGCASTGD 240

Query: 337 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 396
           TSAALSAYCASAGIPSIVFLPA+KISMAQLVQPIANGAFVLSIDTDFDGCM LIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPADKISMAQLVQPIANGAFVLSIDTDFDGCMHLIREVTAE 300

Query: 397 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 456
           LPIYLANSLNSLRLEGQKTAAIEILQQF+W VP+WVI+PGGNLGNIYAFYKGF MC+ELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFNWQVPDWVIVPGGNLGNIYAFYKGFHMCKELG 360

Query: 457 LVDRIPRLVCAQAANANPLYLHYKSGWK-DFKAVKASSTFASAIQIGDPVSIDRAVCALQ 516
           LVDRIPRLVCAQAANANPLYLHYKSG+K DF  +KA++TFASAIQIGDPVSIDRAV AL+
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGFKEDFNPLKANTTFASAIQIGDPVSIDRAVYALK 420

Query: 517 NSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTA 576
            SNGIVEEATEEELMDA A ADSTGMFICPHTGVALTAL+KLR  GVI   DRTVVVSTA
Sbjct: 421 KSNGIVEEATEEELMDATALADSTGMFICPHTGVALTALMKLRKSGVIGANDRTVVVSTA 480

Query: 577 HGLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYL 622
           HGLKFTQSKIDYHSK I +MAC+ ANPPV+VKA +GSVMDVLK+YL
Sbjct: 481 HGLKFTQSKIDYHSKNIKEMACRLANPPVKVKAKFGSVMDVLKEYL 511

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9MT285.6e-25182.49Threonine synthase, chloroplastic OS=Solanum tuberosum OX=4113 PE=2 SV=1[more]
Q9S7B54.1e-24681.63Threonine synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS1 PE=1 ... [more]
Q9SSP54.2e-23077.76Threonine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS2 PE=1 ... [more]
Q588606.1e-7237.12Threonine synthase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 266... [more]
Q8TQD49.4e-6538.87Threonine synthase OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 /... [more]
Match NameE-valueIdentityDescription
KAG6590018.10.0100.00hypothetical protein SDJN03_15441, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022960888.10.099.81threonine synthase, chloroplastic [Cucurbita moschata] >KAG7023681.1 hypothetica... [more]
XP_022987673.10.099.06threonine synthase, chloroplastic [Cucurbita maxima][more]
XP_023515876.10.098.88threonine synthase, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_038878968.10.094.94threonine synthase, chloroplastic-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1HAA40.099.81Threonine synthase OS=Cucurbita moschata OX=3662 GN=LOC111461551 PE=3 SV=1[more]
A0A6J1JK490.099.06Threonine synthase OS=Cucurbita maxima OX=3661 GN=LOC111485156 PE=3 SV=1[more]
A0A5D3CDZ30.093.63Threonine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G00... [more]
A0A5A7UKY20.093.63Threonine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G0... [more]
A0A0A0M2230.093.63Threonine synthase OS=Cucumis sativus OX=3659 GN=Csa_1G590270 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G29840.12.9e-24781.63Pyridoxal-5'-phosphate-dependent enzyme family protein [more]
AT1G72810.13.0e-23177.76Pyridoxal-5'-phosphate-dependent enzyme family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 380..627
e-value: 1.5E-70
score: 239.9
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 157..379
e-value: 7.3E-64
score: 218.0
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeSUPERFAMILY53686Tryptophan synthase beta subunit-like PLP-dependent enzymescoord: 180..620
IPR001926Pyridoxal-phosphate dependent enzymePFAMPF00291PALPcoord: 268..574
e-value: 1.7E-47
score: 162.2
IPR004450Threonine synthase-likeTIGRFAMTIGR00260TIGR00260coord: 256..579
e-value: 2.1E-60
score: 202.7
NoneNo IPR availablePIRSRPIRSR038945-2PIRSR038945-2coord: 248..562
e-value: 2.7E-70
score: 234.9
NoneNo IPR availablePIRSRPIRSR038945-1PIRSR038945-1coord: 248..562
e-value: 2.7E-70
score: 234.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 132..176
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 132..156
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 157..176
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availablePANTHERPTHR10314:SF176THREONINE SYNTHASE, CHLOROPLASTIC-LIKE ISOFORM X1coord: 127..624
NoneNo IPR availablePANTHERPTHR10314CYSTATHIONINE BETA-SYNTHASEcoord: 127..624
NoneNo IPR availableCDDcd01563Thr-synth_1coord: 250..580
e-value: 3.85863E-158
score: 455.129
IPR000634Serine/threonine dehydratase, pyridoxal-phosphate-binding sitePROSITEPS00165DEHYDRATASE_SER_THRcoord: 295..309

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g214540.m01Csor.00g214540.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019344 cysteine biosynthetic process
biological_process GO:0009088 threonine biosynthetic process
biological_process GO:0006520 cellular amino acid metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0004795 threonine synthase activity