Csor.00g209670 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g209670
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	nicotianamine synthase-like
Location	Csor_Chr05: 8843062 .. 8844404 (-)
RNA-Seq Expression	Csor.00g209670
Synteny	Csor.00g209670

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintronterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGGCCCTCTGTTGCGAGAAGTGTGCGCCCTGTACGACCAAATCTCGAGCCTTGCTAGCCTCAAGCCTTCCAAAAATGTCGACACTCTCTTCACCCAACTAGTCCTCACGTGCTCCCCGCCCGCCCCTGGCCTTGATATCGCATCTTTGCCTCAGCCACTTCAAGCCATGAGGGCTCATCTCATCCGTCTATGCGCCACCGCTGAGGCGCTTCTTGAAGTTCATTTCTCTTCCATTTTGGCTTCCTTTGAGAATCCCATTCATAACTTGTCTATCTTTCCTTATTACTCTAATTACTTGAAGCTCTCTCTTCTCGAGTTCGATATTCTTACCTCTCATTGTCGTCGGGTGCCCTCTAGCGTTGCGTTCGTTGGCTCTGGCCCCCTCCCGCTTTCCTCCATTGTCATGGCGTCCACTCATCTTAAGGGTAACTCACACGCTAGTATTACATAACAACATGAGATTATGTCACAAAATTAATCCTCGTCTTTTCGACGTGAGATTCTCAACAGCCTCCCTCGAGATGATCCCAATTTATTTTTGACCGAATACCGGATTCGGGTTCATCGGCTTAGATACCCTATTAGATTAATATCATGTTCATCTTATTATGATCGTGAGAGATTTTCAATTTTTTTTTTTTTTTTTTTTTGTTGTTTTTTTTAATAAGATTTTCGAGAGCTTGCTGGGTTGAGGTGTGATGTGATAGATTATGGTAGATTTGTTATCTAGCTCTAATACCATATTAAGAATAATTCATGAACTTTTTCGTCTTATATTTCTCAATAGGTACTATTTTCCACAACTTTGACATCGACCCAGTTGCAAACTCCATGGCGGGGCGGTTGGTTTGCTCGGACTCAGATTTATCGAAACGGATGGTGTTTCACACGGCAGACGTAATGGAGGTGACGGAGGCGTTTGGAGAGTACGAGGTGGTGTTTTTAGCAGCATTGGTAGGGTTGGAGGAGGAAGAGAAAGGGAGGGTTTTGAGGCATTTAGGGAAGCATATGGCACCCGGAGCTTATTTGATGCTAAGGAGTGCACATGGAGCTAGAGCATTTCTCTACCCTGTTGTTGACGTTCGTGACGTCGAAGCATCTGGGTTCGAGATTTTGTCCGTGTTTCACCCCACTGATGAAGTTATAAACTCCGTTGTTATTGCAAGAAAGGTATCTGATCTCATTCCTGACAACGATAATTATAACAACAACGACACAGATGATGAAAAACGAGGTGTTTTAGTTAACACCCCTATAATATTGGCCGAGAAGTGCTCGGGATTTAAAGACTTCGGTCCCATGATCGAGGAGCTTGCCATAGAAGAGCAACTTTCTTGA

mRNA sequence

ATGGAAGGCCCTCTGTTGCGAGAAGTGTGCGCCCTGTACGACCAAATCTCGAGCCTTGCTAGCCTCAAGCCTTCCAAAAATGTCGACACTCTCTTCACCCAACTAGTCCTCACGTGCTCCCCGCCCGCCCCTGGCCTTGATATCGCATCTTTGCCTCAGCCACTTCAAGCCATGAGGGCTCATCTCATCCGTCTATGCGCCACCGCTGAGGCGCTTCTTGAAGTTCATTTCTCTTCCATTTTGGCTTCCTTTGAGAATCCCATTCATAACTTGTCTATCTTTCCTTATTACTCTAATTACTTGAAGCTCTCTCTTCTCGAGTTCGATATTCTTACCTCTCATTGTCGTCGGGTGCCCTCTAGCGTTGCGTTCGTTGGCTCTGGCCCCCTCCCGCTTTCCTCCATTGTCATGGCGTCCACTCATCTTAAGGGTACTATTTTCCACAACTTTGACATCGACCCAGTTGCAAACTCCATGGCGGGGCGGTTGGTTTGCTCGGACTCAGATTTATCGAAACGGATGGTGTTTCACACGGCAGACGTAATGGAGGTGACGGAGGCGTTTGGAGAGTACGAGGTGGTGTTTTTAGCAGCATTGGTAGGGTTGGAGGAGGAAGAGAAAGGGAGGGTTTTGAGGCATTTAGGGAAGCATATGGCACCCGGAGCTTATTTGATGCTAAGGAGTGCACATGGAGCTAGAGCATTTCTCTACCCTGTTGTTGACGTTCGTGACGTCGAAGCATCTGGGTTCGAGATTTTGTCCGTGTTTCACCCCACTGATGAAGTTATAAACTCCGTTGTTATTGCAAGAAAGGTATCTGATCTCATTCCTGACAACGATAATTATAACAACAACGACACAGATGATGAAAAACGAGGTGTTTTAGTTAACACCCCTATAATATTGGCCGAGAAGTGCTCGGGATTTAAAGACTTCGGTCCCATGATCGAGGAGCTTGCCATAGAAGAGCAACTTTCTTGA

Coding sequence (CDS)

ATGGAAGGCCCTCTGTTGCGAGAAGTGTGCGCCCTGTACGACCAAATCTCGAGCCTTGCTAGCCTCAAGCCTTCCAAAAATGTCGACACTCTCTTCACCCAACTAGTCCTCACGTGCTCCCCGCCCGCCCCTGGCCTTGATATCGCATCTTTGCCTCAGCCACTTCAAGCCATGAGGGCTCATCTCATCCGTCTATGCGCCACCGCTGAGGCGCTTCTTGAAGTTCATTTCTCTTCCATTTTGGCTTCCTTTGAGAATCCCATTCATAACTTGTCTATCTTTCCTTATTACTCTAATTACTTGAAGCTCTCTCTTCTCGAGTTCGATATTCTTACCTCTCATTGTCGTCGGGTGCCCTCTAGCGTTGCGTTCGTTGGCTCTGGCCCCCTCCCGCTTTCCTCCATTGTCATGGCGTCCACTCATCTTAAGGGTACTATTTTCCACAACTTTGACATCGACCCAGTTGCAAACTCCATGGCGGGGCGGTTGGTTTGCTCGGACTCAGATTTATCGAAACGGATGGTGTTTCACACGGCAGACGTAATGGAGGTGACGGAGGCGTTTGGAGAGTACGAGGTGGTGTTTTTAGCAGCATTGGTAGGGTTGGAGGAGGAAGAGAAAGGGAGGGTTTTGAGGCATTTAGGGAAGCATATGGCACCCGGAGCTTATTTGATGCTAAGGAGTGCACATGGAGCTAGAGCATTTCTCTACCCTGTTGTTGACGTTCGTGACGTCGAAGCATCTGGGTTCGAGATTTTGTCCGTGTTTCACCCCACTGATGAAGTTATAAACTCCGTTGTTATTGCAAGAAAGGTATCTGATCTCATTCCTGACAACGATAATTATAACAACAACGACACAGATGATGAAAAACGAGGTGTTTTAGTTAACACCCCTATAATATTGGCCGAGAAGTGCTCGGGATTTAAAGACTTCGGTCCCATGATCGAGGAGCTTGCCATAGAAGAGCAACTTTCTTGA

Protein sequence

MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAHLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPSSVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTADVMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVDVRDVEASGFEILSVFHPTDEVINSVVIARKVSDLIPDNDNYNNNDTDDEKRGVLVNTPIILAEKCSGFKDFGPMIEELAIEEQLS

Homology

[Close]

BLAST of Csor.00g209670 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 1.8e-91
Identity = 167/269 (62.08%), Postives = 217/269 (80.67%), Query Frame = 0


Query: 4   PLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAHLI 63
           P++ +VC LY+QIS L +L PSK+V+ LFT LV TC PP P +D++ L Q +Q +R+HLI
Sbjct: 8   PVVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTCMPPNP-IDVSKLCQKIQEIRSHLI 67

Query: 64  RLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPSSVA 123
           +LC  AE LLE HFS IL+S+ENP+ +L IFPY+ NY+KLSLLE++ILT +   +P  +A
Sbjct: 68  KLCGQAEGLLESHFSKILSSYENPLQHLHIFPYFDNYIKLSLLEYNILTKNTTNIPKKIA 127

Query: 124 FVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTADVME 183
           F+GSGPLPL+S+V+A+ HLK T FHN+DID  AN MA  LV +D D+S RM FHTADVM+
Sbjct: 128 FIGSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSRMTFHTADVMD 187

Query: 184 VTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVDVR 243
           VT A  +Y+VVFLAALVG+++E+K +V+ HL K+M+PGA LMLRSAHGARAFLYPV+D R
Sbjct: 188 VTCALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGARAFLYPVLDPR 247

Query: 244 DVEASGFEILSVFHPTDEVINSVVIARKV 273
           D+   GFE+LSV+HPTDEVINSV+IARK+
Sbjct: 248 DLR--GFEVLSVYHPTDEVINSVIIARKL 273

[Close]

BLAST of Csor.00g209670 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 2.3e-91
Identity = 167/272 (61.40%), Postives = 221/272 (81.25%), Query Frame = 0


Query: 5   LLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAHLIR 64
           L+ ++C LY++IS L +LKP ++VDTLF QLV TC PP P +D+  + + +Q MR++LI+
Sbjct: 9   LVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPNIDVTKMSENIQEMRSNLIK 68

Query: 65  LCATAEALLEVHFSSILASFE-NPIHNLSIFPYYSNYLKLSLLEFDILTSHCRR-VPSSV 124
           +C  AE  LE HFSSIL SFE NP+H+L++FPYY+NYLKLS LEFD+L  +    VP +V
Sbjct: 69  ICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGFVPRTV 128

Query: 125 AFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTADVM 184
           AF+GSGPLPL+S+V+AS+HLK +IFHNFDIDP AN +A RLV SD DLS+RM FHT D+M
Sbjct: 129 AFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFHTVDIM 188

Query: 185 EVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVDV 244
           +VTE+   ++VVFLAALVG++++EK +V+ HL KHM+PGA LMLRSAHG RAFLYP+V+ 
Sbjct: 189 DVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYPIVEP 248

Query: 245 RDVEASGFEILSVFHPTDEVINSVVIARKVSD 275
            D+E  GFE+LSV+HPTDEVINS+VI+RK+ +
Sbjct: 249 CDLE--GFEVLSVYHPTDEVINSIVISRKLGE 278

[Close]

BLAST of Csor.00g209670 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 1.8e-88
Identity = 165/269 (61.34%), Postives = 214/269 (79.55%), Query Frame = 0


Query: 5   LLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAHLIR 64
           L++ +C LY++IS L SLKPS++V+ LF QLV TC PP P +D+  +   +Q +R +LI+
Sbjct: 8   LVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPNIDVTKMCDRVQEIRLNLIK 67

Query: 65  LCATAEALLEVHFSSILASF-ENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRR-VPSSV 124
           +C  AE  LE HFSSIL S+ +NP+H+L+IFPYY+NYLKL  LEFD+L  +    VP SV
Sbjct: 68  ICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLNGFVPKSV 127

Query: 125 AFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTADVM 184
           AF+GSGPLPL+SIV+AS HLK TIFHNFDIDP ANS+A  LV SD D+S+RM FHT D+M
Sbjct: 128 AFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFFHTVDIM 187

Query: 185 EVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVDV 244
           +VTE+   ++VVFLAALVG+ +EEK +V+ HL KHMAPGA LMLRSAHG RAFLYP+V+ 
Sbjct: 188 DVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAFLYPIVEP 247

Query: 245 RDVEASGFEILSVFHPTDEVINSVVIARK 272
            D++  GFE+LS++HPTD+VINSVVI++K
Sbjct: 248 CDLQ--GFEVLSIYHPTDDVINSVVISKK 274

[Close]

BLAST of Csor.00g209670 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 7.7e-87
Identity = 160/271 (59.04%), Postives = 207/271 (76.38%), Query Frame = 0


Query: 5   LLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAHLIR 64
           +++++  LYDQIS L SLKPSKNVDTLF QLV TC P    +D+ ++ + ++ MRA+LI+
Sbjct: 8   VVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTNIDVTNMCEEVKDMRANLIK 67

Query: 65  LCATAEALLEVHFSSILASF---ENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPSS 124
           LC  AE  LE HFS+IL S    +NP+ +L IFPYYSNYLKL  LEFD+L+ H   VP+ 
Sbjct: 68  LCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQHSSHVPTK 127

Query: 125 VAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTADV 184
           +AFVGSGP+PL+SIV+A  HL  T FHNFDID  AN++A  LV  D DLSKRM+FHT DV
Sbjct: 128 IAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIFHTTDV 187

Query: 185 MEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVD 244
           +  TE   +Y+VVFLAALVG+++E K + + HL KHMAPGA LMLRSAH  RAFLYP+VD
Sbjct: 188 LNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIVD 247

Query: 245 VRDVEASGFEILSVFHPTDEVINSVVIARKV 273
             D++  GF++L+++HPTD+V+NSVVIARK+
Sbjct: 248 SSDLK--GFQLLTIYHPTDDVVNSVVIARKL 276

[Close]

BLAST of Csor.00g209670 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 7.7e-87
Identity = 160/270 (59.26%), Postives = 210/270 (77.78%), Query Frame = 0


Query: 5   LLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASL-PQPLQAMRAHLI 64
           +++++  LY+QIS+L SLKPSKNVDTLF QLV TC P    +D+  +  + ++ MR+HLI
Sbjct: 8   VVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLPTDTNIDVTEIHDEKVKDMRSHLI 67

Query: 65  RLCATAEALLEVHFSSILASFE-NPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPSSV 124
           +LC  AE  LE HFS+IL SFE NP+++L IFPYY+NYLKL  LEFD+L+ H   VP+ V
Sbjct: 68  KLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHTTHVPTKV 127

Query: 125 AFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTADVM 184
           AF+GSGP+PL+SIV+A  HL  T FHNFDID  AN++A  LV  DSDLSKRM+FHT DV+
Sbjct: 128 AFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFHTTDVL 187

Query: 185 EVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVDV 244
              E   +Y+VVFLAALVG+++E K + + HL KHMAPGA +MLRSAHG RAFLYP+VD 
Sbjct: 188 NAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYPIVDS 247

Query: 245 RDVEASGFEILSVFHPTDEVINSVVIARKV 273
            D++  GFE+L+++HP+D+V+NSVVIARK+
Sbjct: 248 CDLK--GFEVLTIYHPSDDVVNSVVIARKL 275

[Close]

BLAST of Csor.00g209670 vs. NCBI nr
Match: KAG6599185.1 (Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 637 bits (1643), Expect = 3.79e-230
Identity = 326/326 (100.00%), Postives = 326/326 (100.00%), Query Frame = 0


Query: 1   MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 60
           MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA
Sbjct: 1   MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 60

Query: 61  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 120
           HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS
Sbjct: 61  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 120

Query: 121 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTAD 180
           SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTAD
Sbjct: 121 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTAD 180

Query: 181 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 240
           VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV
Sbjct: 181 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 240

Query: 241 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSDLIPDNDNYNNNDTDDEKRGVLVNTPI 300
           DVRDVEASGFEILSVFHPTDEVINSVVIARKVSDLIPDNDNYNNNDTDDEKRGVLVNTPI
Sbjct: 241 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSDLIPDNDNYNNNDTDDEKRGVLVNTPI 300

Query: 301 ILAEKCSGFKDFGPMIEELAIEEQLS 326
           ILAEKCSGFKDFGPMIEELAIEEQLS
Sbjct: 301 ILAEKCSGFKDFGPMIEELAIEEQLS 326

[Close]

BLAST of Csor.00g209670 vs. NCBI nr
Match: KAG7030119.1 (Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 637 bits (1642), Expect = 5.39e-230
Identity = 325/326 (99.69%), Postives = 326/326 (100.00%), Query Frame = 0


Query: 1   MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 60
           MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA
Sbjct: 1   MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 60

Query: 61  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 120
           HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS
Sbjct: 61  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 120

Query: 121 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTAD 180
           S+AFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTAD
Sbjct: 121 SIAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTAD 180

Query: 181 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 240
           VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV
Sbjct: 181 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 240

Query: 241 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSDLIPDNDNYNNNDTDDEKRGVLVNTPI 300
           DVRDVEASGFEILSVFHPTDEVINSVVIARKVSDLIPDNDNYNNNDTDDEKRGVLVNTPI
Sbjct: 241 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSDLIPDNDNYNNNDTDDEKRGVLVNTPI 300

Query: 301 ILAEKCSGFKDFGPMIEELAIEEQLS 326
           ILAEKCSGFKDFGPMIEELAIEEQLS
Sbjct: 301 ILAEKCSGFKDFGPMIEELAIEEQLS 326

[Close]

BLAST of Csor.00g209670 vs. NCBI nr
Match: XP_022946411.1 (nicotianamine synthase-like [Cucurbita moschata])

HSP 1 Score: 627 bits (1618), Expect = 2.45e-226
Identity = 320/326 (98.16%), Postives = 324/326 (99.39%), Query Frame = 0


Query: 1   MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 60
           MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA
Sbjct: 1   MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 60

Query: 61  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 120
           HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS
Sbjct: 61  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 120

Query: 121 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTAD 180
           SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAG+LVCSDSDLSKRMVFHT D
Sbjct: 121 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTD 180

Query: 181 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 240
           VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV
Sbjct: 181 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 240

Query: 241 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSDLIPDNDNYNNNDTDDEKRGVLVNTPI 300
           DVRDVEASGFEILSVFHPTDEVINSVVIARKVS+L+PDNDNYN+NDTDDEKR VLVNTPI
Sbjct: 241 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSNLVPDNDNYNDNDTDDEKRAVLVNTPI 300

Query: 301 ILAEKCSGFKDFGPMIEELAIEEQLS 326
           ILAEKCSGFKDFGPMIEELAIEEQLS
Sbjct: 301 ILAEKCSGFKDFGPMIEELAIEEQLS 326

[Close]

BLAST of Csor.00g209670 vs. NCBI nr
Match: XP_023545298.1 (nicotianamine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 625 bits (1612), Expect = 2.02e-225
Identity = 318/326 (97.55%), Postives = 323/326 (99.08%), Query Frame = 0


Query: 1   MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 60
           MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA
Sbjct: 1   MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 60

Query: 61  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 120
           HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS
Sbjct: 61  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 120

Query: 121 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTAD 180
           SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDP AN+MAG+LVCSDSDLSKRMVFHTAD
Sbjct: 121 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPAANTMAGQLVCSDSDLSKRMVFHTAD 180

Query: 181 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 240
           VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV
Sbjct: 181 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 240

Query: 241 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSDLIPDNDNYNNNDTDDEKRGVLVNTPI 300
           DVRDVEASGFEILSVFHPTDEVINSVVIARKV+DL+PDNDNYN+NDTDDEKR VLVN PI
Sbjct: 241 DVRDVEASGFEILSVFHPTDEVINSVVIARKVTDLVPDNDNYNDNDTDDEKRAVLVNAPI 300

Query: 301 ILAEKCSGFKDFGPMIEELAIEEQLS 326
           ILAEKCSGFKDFGPMIEELAIEEQLS
Sbjct: 301 ILAEKCSGFKDFGPMIEELAIEEQLS 326

[Close]

BLAST of Csor.00g209670 vs. NCBI nr
Match: XP_022998973.1 (nicotianamine synthase-like, partial [Cucurbita maxima])

HSP 1 Score: 608 bits (1567), Expect = 4.43e-218
Identity = 310/326 (95.09%), Postives = 319/326 (97.85%), Query Frame = 0


Query: 1   MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 60
           MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA
Sbjct: 31  MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 90

Query: 61  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 120
           HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDIL SHCRRVPS
Sbjct: 91  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILISHCRRVPS 150

Query: 121 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTAD 180
           S+AFVGSGPLPLSSIVMASTHLKGTIFHNFDIDP ANSMA RL+ SDSDLSKRMVFHTAD
Sbjct: 151 SIAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPAANSMARRLIRSDSDLSKRMVFHTAD 210

Query: 181 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 240
           VMEVT+AFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV
Sbjct: 211 VMEVTKAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 270

Query: 241 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSDLIPDNDNYNNNDTDDEKRGVLVNTPI 300
           DV D+EASGFEILSVFHPTDEVINSVVIARKVSDL+ DN+NYN+N+TDDEKRGVLVNTPI
Sbjct: 271 DVSDIEASGFEILSVFHPTDEVINSVVIARKVSDLVLDNNNYNDNNTDDEKRGVLVNTPI 330

Query: 301 ILAEKCSGFKDFGPMIEELAIEEQLS 326
           ILAEKCSGFKDFGPMI+EL IEEQLS
Sbjct: 331 ILAEKCSGFKDFGPMIDELGIEEQLS 356

[Close]

BLAST of Csor.00g209670 vs. ExPASy TrEMBL
Match: A0A6J1G3L4 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111450477 PE=3 SV=1)

HSP 1 Score: 627 bits (1618), Expect = 1.19e-226
Identity = 320/326 (98.16%), Postives = 324/326 (99.39%), Query Frame = 0


Query: 1   MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 60
           MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA
Sbjct: 1   MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 60

Query: 61  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 120
           HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS
Sbjct: 61  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 120

Query: 121 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTAD 180
           SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAG+LVCSDSDLSKRMVFHT D
Sbjct: 121 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTD 180

Query: 181 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 240
           VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV
Sbjct: 181 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 240

Query: 241 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSDLIPDNDNYNNNDTDDEKRGVLVNTPI 300
           DVRDVEASGFEILSVFHPTDEVINSVVIARKVS+L+PDNDNYN+NDTDDEKR VLVNTPI
Sbjct: 241 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSNLVPDNDNYNDNDTDDEKRAVLVNTPI 300

Query: 301 ILAEKCSGFKDFGPMIEELAIEEQLS 326
           ILAEKCSGFKDFGPMIEELAIEEQLS
Sbjct: 301 ILAEKCSGFKDFGPMIEELAIEEQLS 326

[Close]

BLAST of Csor.00g209670 vs. ExPASy TrEMBL
Match: A0A6J1KIA7 (nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111493502 PE=3 SV=1)

HSP 1 Score: 608 bits (1567), Expect = 2.14e-218
Identity = 310/326 (95.09%), Postives = 319/326 (97.85%), Query Frame = 0


Query: 1   MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 60
           MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA
Sbjct: 31  MEGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRA 90

Query: 61  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 120
           HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDIL SHCRRVPS
Sbjct: 91  HLIRLCATAEALLEVHFSSILASFENPIHNLSIFPYYSNYLKLSLLEFDILISHCRRVPS 150

Query: 121 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTAD 180
           S+AFVGSGPLPLSSIVMASTHLKGTIFHNFDIDP ANSMA RL+ SDSDLSKRMVFHTAD
Sbjct: 151 SIAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPAANSMARRLIRSDSDLSKRMVFHTAD 210

Query: 181 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 240
           VMEVT+AFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV
Sbjct: 211 VMEVTKAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 270

Query: 241 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSDLIPDNDNYNNNDTDDEKRGVLVNTPI 300
           DV D+EASGFEILSVFHPTDEVINSVVIARKVSDL+ DN+NYN+N+TDDEKRGVLVNTPI
Sbjct: 271 DVSDIEASGFEILSVFHPTDEVINSVVIARKVSDLVLDNNNYNDNNTDDEKRGVLVNTPI 330

Query: 301 ILAEKCSGFKDFGPMIEELAIEEQLS 326
           ILAEKCSGFKDFGPMI+EL IEEQLS
Sbjct: 331 ILAEKCSGFKDFGPMIDELGIEEQLS 356

[Close]

BLAST of Csor.00g209670 vs. ExPASy TrEMBL
Match: A0A0A0LGI4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034530 PE=3 SV=1)

HSP 1 Score: 464 bits (1194), Expect = 5.02e-162
Identity = 250/324 (77.16%), Postives = 275/324 (84.88%), Query Frame = 0


Query: 2   EGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCS---PPAPGLDIASLPQPLQAM 61
           E  LL +VCALY+QISSL+SLKP KNVDTLFTQLVLTCS   PP  G DIASL QPL+AM
Sbjct: 8   EALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASLSQPLRAM 67

Query: 62  RAHLIRLCATAEALLEVHFSSILAS-FENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRR 121
           RAHLI+LCA AEALLE+HFSS+LAS F +PI NLSIFPYYSNYLKLSLLEFDIL SH RR
Sbjct: 68  RAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRSHSRR 127

Query: 122 VPSSVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFH 181
           +P  VAFVGSGPLPLSSIV+AS HLKGTIFHNFDIDP AN+MA +LVCSD DLS+RM+FH
Sbjct: 128 IPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIFH 187

Query: 182 TADVMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLY 241
           T DVMEVT+   +YEVVFLAALVGL EEEKGRVL+HLGKHMA G+YLMLRSAHGARAFLY
Sbjct: 188 TKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFLY 247

Query: 242 PVVDVRDVEASGFEILSVFHPTDEVINSVVIARK--VSDLIPDNDNYNNNDTDDEKRGVL 301
           PVVD+  VEASGFEILSVFHPTDEVINSVVIARK  + D   D D     + +DEK+G+L
Sbjct: 248 PVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFDQSSDED-----EDEDEKQGIL 307

Query: 302 VNTP-IILAEKCSGFKDFGPMIEE 318
           VNT  I+LAEKCSGF  F PMIEE
Sbjct: 308 VNTSSIVLAEKCSGFNGFTPMIEE 326

[Close]

BLAST of Csor.00g209670 vs. ExPASy TrEMBL
Match: A0A5D3BBH0 (Putative nicotianamine synthase 4 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001110 PE=3 SV=1)

HSP 1 Score: 460 bits (1184), Expect = 2.30e-160
Identity = 249/326 (76.38%), Postives = 279/326 (85.58%), Query Frame = 0


Query: 2   EGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCS---PPAP-GLDIASLPQPLQA 61
           E  LL++VCALY+QISSL+SLKP KNVDTLFTQLVLTCS   P AP G D+ASL QPLQA
Sbjct: 12  EALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGFDVASLSQPLQA 71

Query: 62  MRAHLIRLCATAEALLEVHFSSILAS-FENPIHNLSIFPYYSNYLKLSLLEFDILTSHCR 121
           MRAHLI+LCA AEALLE+HFSS+LAS F +PI NLSIFPYYSNYLKLSLLEFDIL SH R
Sbjct: 72  MRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRSHSR 131

Query: 122 RVPSSVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVF 181
           R+P+ VAFVGSGPLPLSSIV+AS HLKGTIFHNFDIDP AN+MA +LVCSD DLS+RM+F
Sbjct: 132 RLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIF 191

Query: 182 HTADVMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFL 241
           HT DVMEVT+   +YEVVFLAALVGLEEEEKGRVL+HLGKHMA G+YLMLRSAHGARAFL
Sbjct: 192 HTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFL 251

Query: 242 YPVVDVRDVEASGFEILSVFHPTDEVINSVVIARKVSDLIPDNDNYNNNDTDDE---KRG 301
           YPVVD+  VEASGFEILSVFHPTDEVINSVVIARK   ++ D  +  + D D+E   K G
Sbjct: 252 YPVVDICTVEASGFEILSVFHPTDEVINSVVIARKK--VVFDQSSVEDEDEDEEEEEKPG 311

Query: 302 VLVNTP-IILAEKCSGFKDFGPMIEE 318
           +LV+T  I+LAEKCSGF  F P+IEE
Sbjct: 312 ILVDTSSIVLAEKCSGFNGFTPIIEE 335

[Close]

BLAST of Csor.00g209670 vs. ExPASy TrEMBL
Match: A0A1S3BXI8 (probable nicotianamine synthase 4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494174 PE=3 SV=1)

HSP 1 Score: 431 bits (1107), Expect = 6.01e-149
Identity = 226/275 (82.18%), Postives = 247/275 (89.82%), Query Frame = 0


Query: 2   EGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCS---PPAP-GLDIASLPQPLQA 61
           E  LL++VCALY+QISSL+SLKP KNVDTLFTQLVLTCS   P AP G D+ASL QPLQA
Sbjct: 12  EALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGFDVASLSQPLQA 71

Query: 62  MRAHLIRLCATAEALLEVHFSSILAS-FENPIHNLSIFPYYSNYLKLSLLEFDILTSHCR 121
           MRAHLI+LCA AEALLE+HFSS+LAS F +PI NLSIFPYYSNYLKLSLLEFDIL SH R
Sbjct: 72  MRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRSHSR 131

Query: 122 RVPSSVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVF 181
           R+P+ VAFVGSGPLPLSSIV+AS HLKGTIFHNFDIDP AN+MA +LVCSD DLS+RM+F
Sbjct: 132 RLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIF 191

Query: 182 HTADVMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFL 241
           HT DVMEVT+   +YEVVFLAALVGLEEEEKGRVL+HLGKHMA G+YLMLRSAHGARAFL
Sbjct: 192 HTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFL 251

Query: 242 YPVVDVRDVEASGFEILSVFHPTDEVINSVVIARK 271
           YPVVD+  VEASGFEILSVFHPTDEVINSVVIARK
Sbjct: 252 YPVVDICTVEASGFEILSVFHPTDEVINSVVIARK 286

[Close]

BLAST of Csor.00g209670 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 337.0 bits (863), Expect = 1.6e-92
Identity = 167/272 (61.40%), Postives = 221/272 (81.25%), Query Frame = 0


Query: 5   LLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAHLIR 64
           L+ ++C LY++IS L +LKP ++VDTLF QLV TC PP P +D+  + + +Q MR++LI+
Sbjct: 9   LVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPNIDVTKMSENIQEMRSNLIK 68

Query: 65  LCATAEALLEVHFSSILASFE-NPIHNLSIFPYYSNYLKLSLLEFDILTSHCRR-VPSSV 124
           +C  AE  LE HFSSIL SFE NP+H+L++FPYY+NYLKLS LEFD+L  +    VP +V
Sbjct: 69  ICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGFVPRTV 128

Query: 125 AFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTADVM 184
           AF+GSGPLPL+S+V+AS+HLK +IFHNFDIDP AN +A RLV SD DLS+RM FHT D+M
Sbjct: 129 AFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFHTVDIM 188

Query: 185 EVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVDV 244
           +VTE+   ++VVFLAALVG++++EK +V+ HL KHM+PGA LMLRSAHG RAFLYP+V+ 
Sbjct: 189 DVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYPIVEP 248

Query: 245 RDVEASGFEILSVFHPTDEVINSVVIARKVSD 275
            D+E  GFE+LSV+HPTDEVINS+VI+RK+ +
Sbjct: 249 CDLE--GFEVLSVYHPTDEVINSIVISRKLGE 278

[Close]

BLAST of Csor.00g209670 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 327.4 bits (838), Expect = 1.3e-89
Identity = 165/269 (61.34%), Postives = 214/269 (79.55%), Query Frame = 0


Query: 5   LLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAHLIR 64
           L++ +C LY++IS L SLKPS++V+ LF QLV TC PP P +D+  +   +Q +R +LI+
Sbjct: 8   LVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPNIDVTKMCDRVQEIRLNLIK 67

Query: 65  LCATAEALLEVHFSSILASF-ENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRR-VPSSV 124
           +C  AE  LE HFSSIL S+ +NP+H+L+IFPYY+NYLKL  LEFD+L  +    VP SV
Sbjct: 68  ICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLNGFVPKSV 127

Query: 125 AFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTADVM 184
           AF+GSGPLPL+SIV+AS HLK TIFHNFDIDP ANS+A  LV SD D+S+RM FHT D+M
Sbjct: 128 AFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFFHTVDIM 187

Query: 185 EVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVDV 244
           +VTE+   ++VVFLAALVG+ +EEK +V+ HL KHMAPGA LMLRSAHG RAFLYP+V+ 
Sbjct: 188 DVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAFLYPIVEP 247

Query: 245 RDVEASGFEILSVFHPTDEVINSVVIARK 272
            D++  GFE+LS++HPTD+VINSVVI++K
Sbjct: 248 CDLQ--GFEVLSIYHPTDDVINSVVISKK 274

[Close]

BLAST of Csor.00g209670 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 322.0 bits (824), Expect = 5.5e-88
Identity = 160/271 (59.04%), Postives = 207/271 (76.38%), Query Frame = 0


Query: 5   LLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAHLIR 64
           +++++  LYDQIS L SLKPSKNVDTLF QLV TC P    +D+ ++ + ++ MRA+LI+
Sbjct: 8   VVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTNIDVTNMCEEVKDMRANLIK 67

Query: 65  LCATAEALLEVHFSSILASF---ENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPSS 124
           LC  AE  LE HFS+IL S    +NP+ +L IFPYYSNYLKL  LEFD+L+ H   VP+ 
Sbjct: 68  LCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQHSSHVPTK 127

Query: 125 VAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTADV 184
           +AFVGSGP+PL+SIV+A  HL  T FHNFDID  AN++A  LV  D DLSKRM+FHT DV
Sbjct: 128 IAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIFHTTDV 187

Query: 185 MEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVD 244
           +  TE   +Y+VVFLAALVG+++E K + + HL KHMAPGA LMLRSAH  RAFLYP+VD
Sbjct: 188 LNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIVD 247

Query: 245 VRDVEASGFEILSVFHPTDEVINSVVIARKV 273
             D++  GF++L+++HPTD+V+NSVVIARK+
Sbjct: 248 SSDLK--GFQLLTIYHPTDDVVNSVVIARKL 276

[Close]

BLAST of Csor.00g209670 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 322.0 bits (824), Expect = 5.5e-88
Identity = 160/270 (59.26%), Postives = 210/270 (77.78%), Query Frame = 0


Query: 5   LLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASL-PQPLQAMRAHLI 64
           +++++  LY+QIS+L SLKPSKNVDTLF QLV TC P    +D+  +  + ++ MR+HLI
Sbjct: 8   VVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLPTDTNIDVTEIHDEKVKDMRSHLI 67

Query: 65  RLCATAEALLEVHFSSILASFE-NPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPSSV 124
           +LC  AE  LE HFS+IL SFE NP+++L IFPYY+NYLKL  LEFD+L+ H   VP+ V
Sbjct: 68  KLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHTTHVPTKV 127

Query: 125 AFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTADVM 184
           AF+GSGP+PL+SIV+A  HL  T FHNFDID  AN++A  LV  DSDLSKRM+FHT DV+
Sbjct: 128 AFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFHTTDVL 187

Query: 185 EVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVDV 244
              E   +Y+VVFLAALVG+++E K + + HL KHMAPGA +MLRSAHG RAFLYP+VD 
Sbjct: 188 NAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYPIVDS 247

Query: 245 RDVEASGFEILSVFHPTDEVINSVVIARKV 273
            D++  GFE+L+++HP+D+V+NSVVIARK+
Sbjct: 248 CDLK--GFEVLTIYHPSDDVVNSVVIARKL 275

[Close]

BLAST of Csor.00g209670 vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 62.0 bits (149), Expect = 1.0e-09
Identity = 32/59 (54.24%), Postives = 43/59 (72.88%), Query Frame = 0


Query: 218 MAPGAYLMLRSAHGARAFLYPVVDVRDVEASGFEILSVFHP--TDEVINSVVIARKVSD 275
           M PGA LMLRSA G R+FLY  VDV   +  GFE+L ++HP  +D  +NSV++ARK++D
Sbjct: 1   MDPGALLMLRSARGLRSFLY--VDVDPCDLKGFEVLEIYHPSMSDGFVNSVMVARKLTD 57

The following BLAST results are available for this feature:

BLAST of Csor.00g209670 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XGI7	1.8e-91	62.08	Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1	[more]
Q9C7X5	2.3e-91	61.40	Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S...	[more]
O80483	1.8e-88	61.34	Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1	[more]
Q9FF79	7.7e-87	59.04	Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1	[more]
Q9FKT9	7.7e-87	59.26	Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1	[more]

BLAST of Csor.00g209670 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6599185.1	3.79e-230	100.00	Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7030119.1	5.39e-230	99.69	Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022946411.1	2.45e-226	98.16	nicotianamine synthase-like [Cucurbita moschata]	[more]
XP_023545298.1	2.02e-225	97.55	nicotianamine synthase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022998973.1	4.43e-218	95.09	nicotianamine synthase-like, partial [Cucurbita maxima]	[more]

BLAST of Csor.00g209670 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G3L4	1.19e-226	98.16	nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111450477 PE=3 S...	[more]
A0A6J1KIA7	2.14e-218	95.09	nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111493502 PE=3 SV=...	[more]
A0A0A0LGI4	5.02e-162	77.16	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034530 PE=3 SV=1	[more]
A0A5D3BBH0	2.30e-160	76.38	Putative nicotianamine synthase 4 isoform X2 OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A1S3BXI8	6.01e-149	82.18	probable nicotianamine synthase 4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10349...	[more]

BLAST of Csor.00g209670 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56430.1	1.6e-92	61.40	nicotianamine synthase 4	[more]
AT1G09240.1	1.3e-89	61.34	nicotianamine synthase 3	[more]
AT5G04950.1	5.5e-88	59.04	nicotianamine synthase 1	[more]
AT5G56080.1	5.5e-88	59.26	nicotianamine synthase 2	[more]
AT4G26483.1	1.0e-09	54.24	nicotianamine synthases	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 12..273 e-value: 1.9E-61 score: 209.6
IPR004298	Nicotianamine synthase	PFAM	PF03059	NAS	coord: 5..273 e-value: 7.3E-117 score: 389.6
IPR004298	Nicotianamine synthase	PANTHER	PTHR32266	NICOTIANAMINE SYNTHASE 3	coord: 4..275
IPR004298	Nicotianamine synthase	PROSITE	PS51142	NAS	coord: 1..276 score: 55.921581

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g209670.m01	Csor.00g209670.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030418	nicotianamine biosynthetic process
molecular_function	GO:0030410	nicotianamine synthase activity